scholarly journals Perspectives for introgression of genetic material of Mexican potato species into potato pre-breeding programs

2020 ◽  
Vol 17 ◽  
pp. 00125
Author(s):  
Vladimir Molianov ◽  
Oleg Vinogradov ◽  
Natalya Ivanayskaya

In the modern changing climate, selective breeding has been essential for increasing production and ensuring stable yields. Potato varieties with different ripening periods are suitable for the conditions of the Middle Volga region. High-yielding varieties resistant to mechanical damage and diseases, varieties with increased heat resistance and a complex of other important features are being created. The emergence of new directions has complicated the solution of breeding programs and required the orgaization of an intermediate stage in this work: the identification and use of special parent forms - carriers of useful qualities. This is a basic, but necessary task when involving a variety of genetic material in the selection process. Research on the topic was carried out in 2017–2019 in the Samara region (RF) in a special shielded area of AGROSTAR LLC in cooperation with experts of the potato farm of VNIIKH FGBNU, MAG LLC (Kinel), Agrocenter Korenevo LLC, and with the participation of experts of Bavaria-Saat GmbH, Germany.


1998 ◽  
Vol 49 (2) ◽  
pp. 153 ◽  
Author(s):  
K. E. Basford ◽  
M. Cooper

Genotype×environment (G×E) interactions complicate selection forbroad adaptation, while their nature and causes need to be understood toutilise and exploit them in selection for specific adaptation. This invitedreview combines an assessment of the literature with the experience we havegained from involvement in wheat breeding and associated research programs toassess (1) the implications of G×E interactions for wheat breeding inAustralia, (2) the impact that research into G E interactions has had onbreeding strategy, and (3) the evidence for impact from this research efforton genetic improvement of crop adaptation. The role of analytical methodologyin this process is considered and some important issues are discussed.There are sufficient examples drawn from wheat breeding in Australia tosuggest that progress in dealing with G×E interactions can be made andseveral of these are presented. They show that impact in plant breedingfollows from achieving an appropriate level of understanding of theenvironmental and genetic factors causing the interactions as well as anassessment of their importance in the target genotype-environment system. Anaccurate definition of the environmental factor(s) contributing to theG×E interactions has been particularly important in determining therelevance of observed differences in plant adaptation to the target populationof environments. From the combination of biological and statistical studies, amore comprehensive understanding of G×E interactions has emerged andcontributed to new concepts and procedures for dealing with them.Distinguishing between what are repeatable and non-repeatable interactions isa key step. Genuine cases of positive specific adaptation observed inmulti-environment trials (METs) can be exploited by appropriately targetedselection strategies, while non-repeatable interactions are accommodated byselection for broad adaptation.The investigation of G×E interactions for grain yield of wheat inAustralia has matured to the point where an understanding of some of theircauses has enabled wheat breeders to exploit positive components of specificadaptation. The experience that has been gained in achieving these advancesindicates the importance of establishing a MET system that is relevant to thetarget population of environments of the breeding program. The investment ofadequate resources into effective design, conduct, analysis, andinterpretation of METs remains critical to continued progress from selectionin complex genotype-environment systems that present large G× Einteractions. Wheat breeders who understand their genetic material and thetarget population of environments can then use the generated information baseto achieve impact from their breeding programs.


2011 ◽  
Vol 40 (No. 1) ◽  
pp. 17-24 ◽  
Author(s):  
Y. Atsmon S ◽  
E. Schwarzbach

The sown wheat acreage in Israel increased from less than 40 000 ha in 1948 to more than 100 000 ha in the seventies and has declined slightly since that time. About 3/4 of the crop are grown in the central part of the country, mostly rain fed, with precipitation of 250–500 mm. The cultivation of durum wheat, originally dominant, has declined since the sixties to a small percentage of the wheat acreage and durum wheat breeding is thus negligible. Local bread wheat breeding started around 1960 independently at three breeding organisations, one of which extensively used genetic material from international breeding programs in Mexico. Since the fifties about 25 cultivars have been released, but only a few are of economic importance now. Breeding considerably increased the yielding potential of cultivars. The recent top cultivar Galil yields about twice as much as cv. Florence Aurore, the leading cultivar in 1970. The national average grain yield rose from 900 kg/ha in 1948 to recently 2.3 t/ha. The highest on-farm yields under favourable conditions are about 9 t/ha. An increasing proportion of the wheat acreage is used for silage, reaching now more than 25%. Yearly fluctuations of yield are considerable and are closely related to rainfall. Data also show an improvement in water utilisation by the crop during the analysed period. The domestic production covered originally only about 12% of the consumption, reaching 47% in the decade 1970–1979, but declined again due to the population growth from 1.2 to 6.5 millions.  


2021 ◽  
Vol 10 (15) ◽  
Author(s):  
Nádia Elígia Nunes Pinto Paracampo ◽  
Luiz Ricardo Santos de Souza ◽  
Jéssica Caporal Almeida ◽  
Osmar Alves Lameira

The active germplasm bank (AGB) of Carapichea ipecacuanha (Brot.) L. Andersson at Embrapa Eastern Amazon, in the city of Belém, PA, was the first of its kind to be opened in Brazil for this endangered medicinal species and holds important accessions for agricultural and reproductive research, including the production of active principles. This study aimed to chemically characterize 42 accessions from that AGB by simultaneously quantifying cephaeline and emetine contents in roots using high-performance liquid chromatography with a diode-array detector (HPLC-DAD). Cephaeline concentrations ranged from ‘undetected’ to 1.76%, whereas emetine concentrations were found between 0.64% and 2.49%. The overall emetine/cephaeline ratio varied from 0.43 to 3.52. The differences among mean concentrations of alkaloids observed by comparing the Scott-Knott test at 5% probability suggest the chemical variation among the samples assessed. Therefore, it is concluded such chemical differences may favor the selection of genetic material for commercial purposes based on the production of emetine and/or cephaeline, likewise may contribute to breeding programs.


2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Eko Hary Pudjiwati, M.P ◽  
Danang Kisowo Jenar

ABSTRACTCorn plants are one of the most important food crops after rice and wheat. To get high corn production factors that can affect one of them is the use of high yielding varieties. In superior varieties there are genes that can make plant growth and production be higher than other varieties. In plant breeding programs to obtain a superior variety, it is necessary to know important information about the influence of the female parent. In general, some characters are controlled by genes in the nucleus, but there are some characters that are controlled by genes in cytoplasmic organelles. The characters controlled by genes in cytoplasmic organelles can be identified by reciprocal crossing. Faculty of Agriculture, University of Borneo Tarakan has a collection of S4 corn (selfing 4) that can be used as genetic material in plant breeding programs. Therefore, research needs to be done to find out the important characters that are controlled by genes outside the nucleus or the influence of female elders. the second results of crossing of maize plants (G2G1 and G4G3 with each reciprocal) showed that in vegetative and generative parameters there were female elders. In the same vegetative character in the two crossing of maize plants which showed the presence of female elders namely plant height, number of leaves and stem diameter. Whereas the same generative characters are the age of flowering females, the number of cob, the location of the cobs, the length of the cobs, the weight of the cobs, the weight of 100 seeds and the weight of the seeds per cob. In addition, it is known that corn plant genotypes show higher F1 mean results as female parents in maize crop crossing on several yield characters (generative), namely genotypes G2 and G4.Keywords: Cytoplasmic Genes, Corn, Effect of Female Elders, Reciprocal Crosses ABSTRAK Tanaman jagung merupakan salah satu tanaman pangan yang terpenting setelah padi dan gandum.. Untuk  mendapatkan produksi jagung yang tinggi faktor yang dapat mempengaruhi salah satunya adalah penggunaan varietas unggul. Pada varietas unggul terdapat gen yang dapat membuat pertumbuhan dan produksi tanaman menjadi lebih tinggi dari varietas yang lain. Dalam program pemuliaan tanaman untuk mendapatkan suatu varietas yang unggul perlu mengeketahui informasi penting tentang pengaruh tetua betina. Pada umumnya beberapa karakter dikendalikan oleh gen dalam kromosan inti, akan tetapi terdapat beberapa karakter yang dikendalikan oleh gen yang ada  di organel sitoplasma. Karakter yang dikendalikan oleh gen di organel sitoplasma dapat diketahui dengan melakukan persilangan resiprokal. Fakultas Pertanian Universitas Borneo Tarakan memiliki koleksi jagung S4 (selfing ke 4) yang dapat digunakan sebagai bahan genetik dalam upaya program pemuliaan tanaman. Maka dari itu, perlu dilakukan penelitian  untuk mengetahui karakter-karakter penting yang dikendalikan oleh gen di luar inti atau pengaruh tetua betina. hasil kedua persilangan tanaman jagung (G2G1 dan G4G3 dengan masing – masing resiproknya) menunjukkan pada parameter vegetatif dan generatif terdapat adanya tetua betina. Pada karakter vegetatif yang sama pada kedua persilangan tanaman jagungyang menunjukkan adanya tetua betina yaitu tinggi tanaman, jumlah daun dan diameter batang. Sedangkan pada karakter generatif yang sama yaitu umur berbunga betina, jumlah tongkol, letak tongkol, panjang tongkol, berat tongkol, berat 100 biji dan berat biji per tongkol. Selain itu, diketahui genotipe tanaman jagung yang menunjukkan hasil nilai rerata F1 yang lebih tinggi sebagai tetua betina pada persilangan tanaman jagung pada beberapa karakter hasil (generatif) yaitu genotipe G2 dan G4. Kata kunci : Gen Sitoplasma, Jagung, Pengaruh Tetua Betina, Persilangan Resiprok


2017 ◽  
Vol 47 (6) ◽  
Author(s):  
Aline Meneguzzi ◽  
Mayra Juline Gonçalves ◽  
Samila Silva Camargo ◽  
Fernanda Grimaldi ◽  
Gabriela Candido Weber ◽  
...  

ABSTRACT: International breeding programs launched new genetic material of apple rootstocks that in addition to precocity and great yield are resistant to major diseases and soil pests encountered in the largest apple producing regions in Brazil. Given this, there is a necessity for vegetative propagation of these materials for study and possible replacement of existing rootstocks. The objective was to adapt a micropropagation protocol for new apple rootstock ‘G. 814’. In the multiplication phase were evaluated BAP concentrations: 0; 0.5; 1; 2 and 4mg L-1 and in the rooting phase were evaluated IBA concentrations: 0; 0.25; 0.50; 1; 1.5 and 2.5mg L-1. These new results demonstrated that this new rootstock selection can be propagated with this tissue culture adapted protocol. For the successful in vitro propagation of apple rootstock ‘G. 814’ it is indicated the use of 1mg L-1 BAP at multiplication phase and 1.5mg L-1 IBA at rooting phase.


2014 ◽  
Vol 6 (3) ◽  
pp. 331-334
Author(s):  
Sali Ali ALIU ◽  
Imer RUSINOVCI ◽  
Shukri FETAHU ◽  
Bekim GASHI

Genetic diversity analysis of common bean populations is useful for breeding programs, as it helps to select genetic material to be used for further crossings. Twenty (20) common bean populations were analyzed using qualitative traits, chlorophyll “a” (Chl ‘a’), chlorophyll “b” (Chl ‘b’), total chlorophyll “a+b” (Total Chl) and carotenoides. The design of the experiment was conducted with leaves of common bean collected from different regions of Kosovo. The experiment was completely randomly with four repetitions. Pigments were extracted by grinding 80-100 mg freshly sampled leaves in 80% (v/v) acetone/water containing MgCO3, at room temperature, preserved in the dark for 24 hours. Concentration of chlorophyll and carotenoid content was measured by spectrophotometer using absorbance recorded at 663 nm, 644 nm and 452.3 nm for maximum absorption of Chl ‘a’, Chl ‘b’, and carotenoids respectively. According to our data the differences between populations for Chl ‘a’, and Chl ‘b’ was significantly higher at level of probability LSDp=0.01. The average values for Chl ‘a’, was 1.67 mg.g-1, while for Chl‘b’was 0.74 mg.g-1. In addition, the results for carotenoids content between populations were with high differences.


2020 ◽  
Vol 10 (18) ◽  
pp. 6414
Author(s):  
Nuno Rodrigues ◽  
Teresa Pinho ◽  
Susana Casal ◽  
António M. Peres ◽  
Paula Baptista ◽  
...  

Oleaster (Olea europaea var. sylvestris), or the wild olive tree, has great interest as a source of genetic material for olive breeding programs. Nevertheless, information about its oil composition is scarce. In the present work, the characterization of oleaster fruit morphology and oil chemical composition from three different tree populations in Northeastern Portugal (Moncorvo, Alijó and Vila Nova de Foz Côa) was performed. The three studied populations presented some morphological differences in the fruits, but similar oil chemical composition. Oleic acid (68.9–70.6%) was the most abundant fatty acid. High variability was observed in total tocopherol content, ranging between 263 and 503 mg/kg. Additionally, high amounts of total sterols were found, from 1742 to 2198 mg/kg of oil. A rich composition in phenols was found with 14 phenolic compounds identified. The evaluated parameters for oleander oils allowed discriminating the oils according to the geographical origin and were consistent with those commonly found in olive oil, showing that they are particularly rich in antioxidants and can be exploited in breeding programs to increase the amounts of bioactive compounds in cultivated oils.


Genes ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 1109
Author(s):  
Andrea Mazzatenta ◽  
Massimo Vignoli ◽  
Maurizio Caputo ◽  
Giorgio Vignola ◽  
Roberto Tamburro ◽  
...  

The mitochondrial DNA (mtDNA) D-loop of endangered and critically endangered breeds has been studied to identify maternal lineages, characterize genetic inheritance, reconstruct phylogenetic relations among breeds, and develop biodiversity conservation and breeding programs. The aim of the study was to determine the variability remaining and the phylogenetic relationship of Martina Franca (MF, with total population of 160 females and 36 males), Ragusano (RG, 344 females and 30 males), Pantesco (PT, 47 females and 15 males), and Catalonian (CT) donkeys by collecting genetic data from maternal lineages. Genetic material was collected from saliva, and a 350 bp fragment of D-loop mtDNA was amplified and sequenced. Sequences were aligned and evaluated using standard bioinformatics software. A total of 56 haplotypes including 33 polymorphic sites were found in 77 samples (27 MF, 22 RG, 8 PT, 19 CT, 1 crossbred). The breed nucleotide diversity value (π) for all the breeds was 0.128 (MF: 0.162, RG: 0.132, PT: 0.025, CT: 0.038). Principal components analysis grouped most of the haplogroups into two different clusters, I (including all haplotypes from PT and CT, together with haplotypes from MF and RG) and II (including haplotypes from MF and RG only). In conclusion, we found that the primeval haplotypes, haplogroup variability, and a large number of maternal lineages were preserved in MF and RG; thus, these breeds play putative pivotal roles in the phyletic relationships of donkey breeds. Maternal inheritance is indispensable genetic information required to evaluate inheritance, variability, and breeding programs.


2021 ◽  
Vol 22 (10) ◽  
Author(s):  
Zulfahmi Zulfahmi ◽  
Parjanto Parjanto ◽  
Edi Purwanto ◽  
Ahmad Yunus

Abstract. Zulfahmi, Parjanto, Purwanto E, Yunus A. 2021. Genetic diversity and population structure of Eurycoma apiculata in Eastern Sumatra, Indonesia. Biodiversitas 22: 4431-4439. Information on genetic variation within and among populations of Eurycoma apiculata plants is important to develop strategies for their conservation, sustainable use, and genetic improvement. To date, no information on genetic variation within and among populations of the E. apiculata has been reported. This study aims to assess genetic diversity within and among populations of E. apiculata based on RAPD markers, and to determine populations to collect E. apiculata genetic material for conservation and breeding programs. Young leaves of E. apiculata were collected from six natural populations. Fifteen RAPD primers were used to assess the genetic diversity of each population. The data obtained were analyzed with POPGEN and Arlequin software. The amplification results of 15 selected primers produced 3-16 loci with all primers 100% polymorphic. At the species level, the mean allele per locus (Na), number of effective alleles (Ne), percentage of polymorphic loci (PPL), Nei’s gene diversity index (He) and Shannon information index (I) were 2.000, 1.244, 100%, 0.167, and 0.286, respectively. At the population level, the mean values for Na, Ne, PPL, He and I were 1.393, 1.312, 39.27%, 0.119, and 0.186, respectively. The highest value of gene diversity within population (He) was found in the Lingga-1 population and the lowest value was found in the Rumbio population. The value of genetic differentiation among populations (GST) of E. apiculata is 0.284, consistent with the results of the AMOVA analysis which found that genetic variation among populations was 23.14%, indicates that the genetic variation of E. apiculata was more stored within populations than among populations. The gene flow (Nm) value of E. apiculata was 1.259 migrants per generation among populations. The Nm value of this species was high category, and could inhibit genetic differentiation among populations. The clustering of E. apiculata population based on the UPGMA dendrogram and PCA was inconsistent with its geographic distribution, reflecting the possibility that genes migration occurred between islands in the past. The main finding of this study was the genetic variation of the E. apiculata mostly stored within the population. Therefore, the population with the highest genetic diversity is a priority for in-situ conservation, and collection of E. apiculata genetic material for ex-situ conservation and breeding programs should be carried out minimum from Lingga-1 and Pokomo populations.


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