scholarly journals Maternal Phylogenetic Relationships and Genetic Variation among Rare, Phenotypically Similar Donkey Breeds

Genes ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 1109
Author(s):  
Andrea Mazzatenta ◽  
Massimo Vignoli ◽  
Maurizio Caputo ◽  
Giorgio Vignola ◽  
Roberto Tamburro ◽  
...  
Keyword(s):  
Nucleotide Diversity ◽  
Total Population ◽  
Maternal Inheritance ◽  
Genetic Material ◽  
Breeding Programs ◽  
Phylogenetic Relations ◽  
Bioinformatics Software ◽  
D Loop ◽  
Components Analysis ◽  
Relationship Of

The mitochondrial DNA (mtDNA) D-loop of endangered and critically endangered breeds has been studied to identify maternal lineages, characterize genetic inheritance, reconstruct phylogenetic relations among breeds, and develop biodiversity conservation and breeding programs. The aim of the study was to determine the variability remaining and the phylogenetic relationship of Martina Franca (MF, with total population of 160 females and 36 males), Ragusano (RG, 344 females and 30 males), Pantesco (PT, 47 females and 15 males), and Catalonian (CT) donkeys by collecting genetic data from maternal lineages. Genetic material was collected from saliva, and a 350 bp fragment of D-loop mtDNA was amplified and sequenced. Sequences were aligned and evaluated using standard bioinformatics software. A total of 56 haplotypes including 33 polymorphic sites were found in 77 samples (27 MF, 22 RG, 8 PT, 19 CT, 1 crossbred). The breed nucleotide diversity value (π) for all the breeds was 0.128 (MF: 0.162, RG: 0.132, PT: 0.025, CT: 0.038). Principal components analysis grouped most of the haplogroups into two different clusters, I (including all haplotypes from PT and CT, together with haplotypes from MF and RG) and II (including haplotypes from MF and RG only). In conclusion, we found that the primeval haplotypes, haplogroup variability, and a large number of maternal lineages were preserved in MF and RG; thus, these breeds play putative pivotal roles in the phyletic relationships of donkey breeds. Maternal inheritance is indispensable genetic information required to evaluate inheritance, variability, and breeding programs.

scholarly journals Maternal ancestry analyses of red tilapia strains based on D-loop sequences of seven tilapia populations

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7007
Author(s):  
Bingjie Jiang ◽  
Jianjun Fu ◽  
Zaijie Dong ◽  
Min Fang ◽  
Wenbin Zhu ◽  
...  
Keyword(s):  
Genetic Relationship ◽  
Nucleotide Diversity ◽  
Haplotype Diversity ◽  
Important Food ◽  
Red Tilapia ◽  
The Gift ◽  
D Loop ◽  
Food Fish ◽  
Genetic Structures ◽  
Relationship Of

Background Many tilapia species or varieties have been widely introduced and have become an economically important food fish in China. Information on the genetic backgrounds of these populations is deficient and requires more research, especially for red tilapia strains. Methods In the present study, displacement loop (D-loop) sequences were used to evaluate the genetic relationship and diversity of seven tilapia populations that are widely cultured in China; this was done specifically to speculate on the maternal ancestry of red tilapia strains. Three red tilapia varieties of Oreochromis ssp., Taiwan (TW), Israel (IL), and Malaysia (MY) strains and other populations, including O. aureus (AR), O. niloticus (NL), O. mossambicus (MS), and the GIFT strain of O. niloticus, were collected and analyzed in this study. Results A total of 146 polymorphic sites and 32 haplotypes of D-loop sequences were detected among 332 fish and four major haplotypes were shared among the populations. The TW and NL populations had a greater number of haplotypes (20 and 8, respectively). The haplotype diversity (Hd) and nucleotide diversity (π) of each population ranged from 0.234 to 0.826, and 0 to 0.060, respectively. The significant positive Tajima’s D value of neutral test were detected in the NL, IL, and MY populations (P < 0.05), which indicated these populations might have not experienced historical expansion. According to the pairwise F-statistics, highly significant genetic differentiations were detected among populations (P < 0.01), with the exception of the IL and MY populations (P > 0.05). The nearest K2P genetic distance (D = 0.014) was detected between the MS and TW populations, whereas, the farthest (D = 0.101) was found between the GIFT and AR populations. The results from the molecular variance analysis (AMOVA) showed that there was an extremely significant genetic variation observed among the populations (P < 0.01), which contained 63.57% of the total variation. In view of the genetic relationship of red tilapia strains with other populations, TW and IL were detected with more similar genetic structures related to MS, and MY was more genetically similar to GIFT (or NL), which could provide more genetic evidence for the red tilapia strains maternal ancestry.

scholarly journals Maternal phylogenetic relationships and genetic variation among rare similar phenotype donkey breeds

2020 ◽  
Author(s):  
Andrea Mazzatenta ◽  
Maurizio Caputo ◽  
Francesco De Sanctis ◽  
Jordi Mirò Roig ◽  
Domenico Robbe ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Phylogenetic Relationships ◽  
Maternal Inheritance ◽  
Historical Records ◽  
Breeding Programs ◽  
Phylogenetic Relations ◽  
Genetic Status ◽  
Intraspecific Genetic Diversity ◽  
Similar Phenotype

AbstractMaternal inheritance is an indispensable aspect in donkey rare breed population biodiversity management and breeding programs. It is a challenge to characterize breeds genetic inheritance using morphology and historical records, we study mtDNA, to overcome those limitations. The mitochondrial DNA (mtDNA) sequencing is a highly informative system to investigate maternal lineages and breed linkage such as molecular evolution and phylogenetic relationships. Martina Franca, Ragusano, Pantesco and Catalonian donkey mtDNA sequencing analyses were used to study intraspecific genetic diversity and population structure, and to reconstruct phylogenetic relations among these geographically isolated breeds.A wide lost in variability among all breeds emerged. In this scenario, the primeval haplotypes, higher haplogroups variability and larger number of maternal lineages are preserved in Martina Franca and Ragusano. Accordingly, a putative pivotal role in the phyletic relationship is likely for such breeds.Given the level of endangerment undergone by these breeds, some actions are necessary to ensure their longtime survival and conservation. Improving the reproduction and management of existing populations, clarifying their historic interactions by studying the genetic status of their populations, extending and improving monitoring maternal lineages represent valid options.

Relationship of Religiosity to Death Anxiety of Middle-Aged Males

1984 ◽  
Vol 54 (3) ◽  
pp. 811-822 ◽  
Author(s):  
Ann M. Downey
Keyword(s):  
Principal Components Analysis ◽  
Principal Components ◽  
Death Anxiety ◽  
Control Variable ◽  
Fear Of Death ◽  
Curvilinear Relationship ◽  
Middle Aged ◽  
Probability Sample ◽  
Components Analysis ◽  
Relationship Of

The purpose of this study was to determine how religiosity was related to death anxiety among a group of middle-aged men. A non-probability sample of 237 male volunteers between the ages of 40 and 59 yr. of whom 88.7% were engaged in professional occupations completed a questionnaire composed of various research instruments. A single composite score for religiosity was obtained through a principal-components analysis of 13 items selected, adjusted, and compiled by Downey (1980) from various religiosity scales. Boyar's Fear of Death Scale assessed death anxiety, while “experience of death” was measured by three items and was utilized as a control variable in determining the association between religiosity and death anxiety. Analysis indicated that “experience of death” or the amount of contact the male has had with death was not related to death anxiety. Data did not support the prediction that those males who were less religious would exhibit higher scores on death anxiety than would those men who were more religious. Further analyses demonstrated a curvilinear relationship between religiosity and death anxiety. The middle-aged men who were moderately religious evidenced a significantly higher fear of death than the men who were either low or high in religiosity.

scholarly journals Hubungan Kekerabatan Sapi Aceh dengan Menggunakan Daerah Displacement-loop

2008 ◽  
Vol 8 (2) ◽  
pp. 9-14 ◽  
Author(s):  
Mohd. Agus Nashri Abdullah
Keyword(s):  
Whole Blood ◽  
Bos Indicus ◽  
Loop Analysis ◽  
Blood Samples ◽  
Bos Javanicus ◽  
Pcr Product ◽  
Two Samples ◽  
D Loop ◽  
Whole Blood Samples ◽  
Relationship Of

Relationship of aceh cattle using displacement-loop regionABSTRACT. The aims of this study were to describe relationship of D-loop of mtDNA Aceh cattle which is useful database for conducting conservation programme. The whole blood samples were collected (8 samples for D-loop analysis) from four locations which were Aceh Besar, Pidie, North Aceh regencies and Banda Aceh city. Out group whole blood samples were collected from two samples from Bali cattles (Bali Island), Madura cattle (Madura Island), Pesisir cattle (West Sumatera) respectively and one sample from PO cattle (West Java). Amplification of D-loop sequences of mtDNA with BIDLF and BIDLR primary have PCR product 980 bp. The Data were analyzed using Squint 1.02 and MEGA 4.0 programme. Result of analysis indicate that Aceh cattle have nearer relationship with zebu and there is items inset of genetik Bali cattle (Bos javanicus) at the end sequences start ke-354 situs up to 483, so that the origin Aceh cattle was from Bos indicus which have hybridization with Bos javanicus.

scholarly journals HUBUNGAN POLA ASUH ORANG TUA TERHADAP PERILAKU BULLYING DI SMA N 1 BOLANGITANG

2019 ◽  
Author(s):  
fendi ntobuo
Keyword(s):  
Parenting Style ◽  
Significant Relationship ◽  
Total Population ◽  
P Value ◽  
Chi Square ◽  
Study Approach ◽  
A Value ◽  
Bullying Behavior ◽  
Relationship Of ◽  
The Relationship

One of negative behavior that is mostly done by adolescents is bullying, this behavior is prevalent in the school enviroment. Generally, the factor that influence bullying behavior are family factors, shool factor, peer factor. The focus was on the influence of parenting style because it has a correlation between parenting on an aggressive behavior. The purpose of this study was to determine the relationship of parenting to bullying behavior in Bolangitan 1 N Senior High study approach. The total population was 48 respondents. The result of study by using the Chi Square statistical tes tah there is a significant relationship between parenting of bullying behavior in Bolangitan N 1 School with a value (p value: 0,000). It can concluded there is a significant relationship between parenting style towards bullying behavior in Bolangitan 1 Senior High School. It suggest that counseling can be held about bullying behavior and its response to adolescents.

scholarly journals HUBUNGAN KECERDASAN EMOSIONAL DENGAN PRESTASI MAHASISWA PRODI D III KEBIDANAN CURUP POLTEKKES KEMENKES BENGKULU

2018 ◽  
Vol 9 (2) ◽  
pp. 200-203
Author(s):  
Lydia Febrina Sipahutar
Keyword(s):  
Experimental Data ◽  
Academic Achievement ◽  
Student Achievement ◽  
Emotional Intelligence ◽  
Data Collection ◽  
Learning Process ◽  
Significant Relationship ◽  
Total Population ◽  
Surrounding Environment ◽  
Relationship Of

During this time many people were found to achieve the required high learningachievement intellectual intelligence (IQ) is also high. However, EQ good can determine thesuccess of individuals in learning achievement in building a successful career, and can reducethe aggressiveness, especially among teenagers purpose of this study was to determinewhether there is a relationship of emotional intelligence toward student learning achievementProdi DIII Midwifery Curup Semester II and IV. This research was conducted in the ProdiDIII Midwifery Curup from July to August 2016, with the number of respondents, thisresearch is descriptive analytic, data collection using a scale based on the theory of emotionalintelligence Daniel Goleman; to measure student achievement used methods of examinationof documents by the second half saw the value of IP, IV and population II and IV semesterstudent, taken by total population, the data was analyzed by univariate and bivariate. Theresults of the analysis of experimental data showed correlation coefficient of 0.635 with p0.005 (<0.05), the Ha accepted. This means that there is a significant relationship betweenemotional intelligence and academic achievement of students Prodi DIII Midwifery Curup IIand IV semester of 2016. To develop and optimize the emotional intelligence plays a role instudent success both in school and in the surrounding environment, it is recommended to thecampus, especially the lecturer-dosenagar incorporate elements emosioal intelligence inpresenting the material as well as the emotions involved in the learning process

scholarly journals Genetic diversity study in Hungarian coldblooded horses

2017 ◽  
pp. 29-34
Author(s):  
Nikolett Csizmár ◽  
Sándor Mihók ◽  
András Jávor ◽  
Szilvia Kusza
Keyword(s):  
High Performance ◽  
Nucleotide Diversity ◽  
Base Pairs ◽  
Loop Region ◽  
Horse Breeding ◽  
D Loop ◽  
Hungarian Population ◽  
Genetic Diversity Study ◽  
Feeding Technology ◽  
Diversity Study

Because of the feeding technology innovation, accelerated transport and communication facilities breeds of high performance breeds replaced local autochone breeds worldwide. These latter species however have an important role in gene conservation. Hungarian cold-blooded horse breeding stock are lacking pedigree, the actual founder breed mares are not known. For this reason, it is an major priority defining the genetic backround of the existing flock, for that breeding could operate with purposeful using of origin maternal founders. In the present study 195 cold-blooded Hungarian mares tail and mane sample were analized. Our analysis was carried out between 15531–15752 base pairs in mithocrondial DNA D-loop region, which reported a total of 222 base pairs. Fourtyone polymorphic sites were determined, which resulted in 39 haplotypes (h=39). The average pairwise differences were k=6.825. High haplotype and nucleotide diversity values were observed (Hd=0.968±0.003, π=0.026±0.003). Based on the previously defined variable positions of haplotypes defined by Jansen et al (2002), we groupped our haplotypes into haplogroups. 23 percent of the studied population (45 mares) belonged to haplogroup F1. Nearly 97% of the analyzed population was classified into one of eight  haplogroups defined by Jansen.et al. (2002). This study gives genetic information nearly 25% of the Hungarian population. Another possibility would be patterning more mares or involving more genetic marker in the study which will assuming the possibility of a more comprehensive analysis.

scholarly journals Analysis and selection of parental forms for crossbreeding of early potatoes for the arid conditions of the Middle Volga region

2020 ◽  
Vol 17 ◽  
pp. 00125
Author(s):  
Vladimir Molianov ◽  
Oleg Vinogradov ◽  
Natalya Ivanayskaya
Keyword(s):  
Selection Process ◽  
Genetic Material ◽  
Mechanical Damage ◽  
Intermediate Stage ◽  
Volga Region ◽  
Middle Volga Region ◽  
Breeding Programs ◽  
Middle Volga ◽  
High Yielding Varieties ◽  
Selection Of

In the modern changing climate, selective breeding has been essential for increasing production and ensuring stable yields. Potato varieties with different ripening periods are suitable for the conditions of the Middle Volga region. High-yielding varieties resistant to mechanical damage and diseases, varieties with increased heat resistance and a complex of other important features are being created. The emergence of new directions has complicated the solution of breeding programs and required the orgaization of an intermediate stage in this work: the identification and use of special parent forms - carriers of useful qualities. This is a basic, but necessary task when involving a variety of genetic material in the selection process. Research on the topic was carried out in 2017–2019 in the Samara region (RF) in a special shielded area of AGROSTAR LLC in cooperation with experts of the potato farm of VNIIKH FGBNU, MAG LLC (Kinel), Agrocenter Korenevo LLC, and with the participation of experts of Bavaria-Saat GmbH, Germany.

Genotype×environment interactions and some considerations of their implications for wheat breeding in Australia This review is one of a series commissioned by the Advisory Committee of the Journal.

1998 ◽  
Vol 49 (2) ◽  
pp. 153 ◽  
Author(s):  
K. E. Basford ◽  
M. Cooper
Keyword(s):  
Genetic Material ◽  
Wheat Breeding ◽  
Breeding Programs ◽  
Specific Adaptation ◽  
New Concepts ◽  
Statistical Studies ◽  
Definition Of ◽  
Level Of Understanding ◽  
The Impact

Genotype×environment (G×E) interactions complicate selection forbroad adaptation, while their nature and causes need to be understood toutilise and exploit them in selection for specific adaptation. This invitedreview combines an assessment of the literature with the experience we havegained from involvement in wheat breeding and associated research programs toassess (1) the implications of G×E interactions for wheat breeding inAustralia, (2) the impact that research into G E interactions has had onbreeding strategy, and (3) the evidence for impact from this research efforton genetic improvement of crop adaptation. The role of analytical methodologyin this process is considered and some important issues are discussed.There are sufficient examples drawn from wheat breeding in Australia tosuggest that progress in dealing with G×E interactions can be made andseveral of these are presented. They show that impact in plant breedingfollows from achieving an appropriate level of understanding of theenvironmental and genetic factors causing the interactions as well as anassessment of their importance in the target genotype-environment system. Anaccurate definition of the environmental factor(s) contributing to theG×E interactions has been particularly important in determining therelevance of observed differences in plant adaptation to the target populationof environments. From the combination of biological and statistical studies, amore comprehensive understanding of G×E interactions has emerged andcontributed to new concepts and procedures for dealing with them.Distinguishing between what are repeatable and non-repeatable interactions isa key step. Genuine cases of positive specific adaptation observed inmulti-environment trials (METs) can be exploited by appropriately targetedselection strategies, while non-repeatable interactions are accommodated byselection for broad adaptation.The investigation of G×E interactions for grain yield of wheat inAustralia has matured to the point where an understanding of some of theircauses has enabled wheat breeders to exploit positive components of specificadaptation. The experience that has been gained in achieving these advancesindicates the importance of establishing a MET system that is relevant to thetarget population of environments of the breeding program. The investment ofadequate resources into effective design, conduct, analysis, andinterpretation of METs remains critical to continued progress from selectionin complex genotype-environment systems that present large G× Einteractions. Wheat breeders who understand their genetic material and thetarget population of environments can then use the generated information baseto achieve impact from their breeding programs.

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