scholarly journals Could the Three Be Edible and Natural Sources of Levodopa? Morphological Characterization of Three Taxa of Mucuna (Fabaceae) in Ebonyi State, Southeastern Nigeria

Author(s):  
O.E. Nwankwo ◽  
S.A. Odewo ◽  
B.A. Ajani ◽  
L.T. Soyewo ◽  
M.S. Nwefuru

Background: There were reports that M. pruriens was edible and natural source of Levodopa used in the treatment of Parkinson’s disease, but the identity of the variety used for the treatment is uncertain. Hence, the present study is important to provide their diagnostic characters for further studies by prospective researchers. Methods: The qualitative study was carried out by observing and recording the features of the taxa while the quantitative was obtained as the leaflet length and width were measured using a metre rule. From each specimen, leaves were randomly selected and measured using metre rule. Standard methods were used in this study. Result: The three taxa possess trifoliate leaves in common and the flower colours are as follows: Yellow in M. flagellipes, purple in M. puriens var. pruriens and white in M. pruriens var. utilis. Testa colours are black, black and white in the M. flagellipes, M. pruriens var. pruriens and M. pruriens var. utilis, respectively. The highest average leaflet length was 17.5 recorded in M. pruriens var. pruriens while the lowest average leaflet length of 8.4 was recorded in M. pruriens var. utilis. The three taxa could be eaten based on review. Petal and testa colour could be used to distinguish the taxa studied.

Author(s):  
Ahmed Seid ◽  
Kefelegn Kebede ◽  
Kefena Effa

SummaryAn exploratory field survey was conducted in Horro Guduru Wollega Zone, Ethiopia, to phenotypically characterize indigenous goats. Eight qualitative and fifteen quantitative traits from 612 goats were considered. All data were analysed using SAS 9.2, version 2008. The dominant coat colour types in Guduru district were black (35.29 percent), whereas in Amuru district, the dominant coat colour types were white and brown with brown dominant (18.63 percent) and brown (17.65 percent). In Horro district, the dominant coat colour types were grey (21.57 percent) and black and white with black dominant (15.69 percent). Morphometric measurements (body weight (BW), heart girth and body length) indicated that the Amuru and Horro goats were significantly (P< 0.05) higher than the Guduru goats. Male goats were consistently higher than female goats in all variables except pelvic width (PW). BW could be predicted from the regression equationy= −45.22 + 1.04xfor does andy= −59.71 + 1.25xfor bucks, whereyandxare the BW and the heart girth, respectively. This phenotypic information serves as a basis for designing appropriate conservation and breeding strategies for goats in the study area. However, it should be substantiated with genetic characterization to guide the overall goat breeding and conservation programmes.


Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.


Planta Medica ◽  
2010 ◽  
Vol 76 (05) ◽  
Author(s):  
APPR Amarasinghe ◽  
RP Karunagoda ◽  
DSA Wijesundara

2020 ◽  
Vol 13 (11) ◽  
pp. 1
Author(s):  
A. R. B. Zanco ◽  
A. Ferreira ◽  
G. C. M. Berber ◽  
E. N. Gonzaga ◽  
D. C. C. Sabino

The different integrated production systems can directly interfere with its bacterial community. The present study aimed to assess density, bacterial diversity and the influence of dry and rainy season in different integrated and an exclusive production system. The fallow and a native forest area was assessed to. Samples were collected in 2012 March and September. The isolation were carried out into Petri dishes containing DYGS medium. The number of colony forming units (CFU) was counted after 48 hours and. The bacterial density ranged between 106 and 107 CFU g-1 soil. The crop system affected the dynamics of the bacterial community only in the rainy season. The rainy season showed greater density of total bacteria when compared to the dry period regardless of the cropping system. The dendrograms with 80 % similarity showed thirteen and fourteen groups in the rainy and dry seasons. Isolates with the capacity to solubilize phosphate in vitro were obtained from all areas in the two seasons, but this feature has been prevalent in bacteria isolated during the rainy season


2020 ◽  
Vol 20 (4) ◽  
pp. 448-454
Author(s):  
Rahmita Burhamzah ◽  
Gemini Alam ◽  
Herlina Rante

Background: Endophytic fungi live in plants’ tissue and can produce the same bioactive compounds as its host plant produces. Syzygiumpolyanthum leaves have known to be one of the antibacterial compound producers. Aim and Objective: This study aimed to characterize morphologically, microscopically, and molecularly the antibacterial-producing endophytic fungi of Syzygiumpolyanthum leaves. Methods: The isolation of endophytic fungi was done by fragment planting method on PDA medium. The antibacterial screening was performed using the antagonistic test as the first screening followed by the disc diffusion test method. The morphological characterization was based on isolate’s mycelia color, growth pattern, margin, and surface texture of the colony, while the microscopic characterization was based on its hyphae characteristics. The molecular characterization of the isolate was done by nitrogen base sequence analysis method on nucleotide constituent of ITS rDNA genes of the isolate. Results: The results found that isolate DF1 has antibacterial activity against E.coli, S.aureus, P.acne, and P.aeruginosa, with the greatest inhibition at 10% concentration of broth fermentation extract on S.aureus with a diameter of inhibition of 13.77 mm. Conclusion: Based on macroscopic, microscopic, and molecular characterization, DF1 isolate is similar to Ceriporialacerate.


Membranes ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 303
Author(s):  
Rokayya Sami ◽  
Schahrazad Soltane ◽  
Mahmoud Helal

In the current work, the characterization of novel chitosan/silica nanoparticle/nisin films with the addition of nisin as an antimicrobial technique for blueberry preservation during storage is investigated. Chitosan/Silica Nanoparticle/N (CH-SN-N) films presented a stable suspension as the surface loads (45.9 mV) and the distribution was considered broad (0.62). The result shows that the pH value was increased gradually with the addition of nisin to 4.12, while the turbidity was the highest at 0.39. The content of the insoluble matter and contact angle were the highest for the Chitosan/Silica Nanoparticle (CH-SN) film at 5.68%. The use of nano-materials in chitosan films decreased the material ductility, reduced the tensile strength and elongation-at-break of the membrane. The coated blueberries with Chitosan/Silica Nanoparticle/N films reported the lowest microbial contamination counts at 2.82 log CFU/g followed by Chitosan/Silica Nanoparticle at 3.73 and 3.58 log CFU/g for the aerobic bacteria, molds, and yeasts population, respectively. It was observed that (CH) film extracted 94 regions with an average size of 449.10, at the same time (CH-SN) film extracted 169 regions with an average size of 130.53. The (CH-SN-N) film presented the best result at 5.19%. It could be observed that the size of the total region of the fruit for the (CH) case was the smallest (1663 pixels), which implied that the fruit lost moisture content. As a conclusion, (CH-SN-N) film is recommended for blueberry preservation to prolong the shelf-life during storage.


2021 ◽  
Author(s):  
Herlina Damayanti ◽  
Kristanto Wahyudi ◽  
Karlina Noordiningsih ◽  
Ayu Ratnasari ◽  
Devi Rianti

Author(s):  
N R Gazizova ◽  
A G Mannapov ◽  
V N Sattarov ◽  
V G Semenov ◽  
A I Skvortsov ◽  
...  

2021 ◽  
Vol 69 (7) ◽  
pp. 2226-2235
Author(s):  
Greta Canelli ◽  
Patricia Murciano Martínez ◽  
Sean Austin ◽  
Mark E. Ambühl ◽  
Fabiola Dionisi ◽  
...  

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