Effect of mannan-oligosaccharides (MOS) supplementation on performance, immunity and HSP70 gene expression in broiler chicken during hot-dry summer

Author(s):  
J. J. Rokade ◽  
Mukesh Kagate ◽  
S. K. Bhanja ◽  
M. Mehra ◽  
A. Goel ◽  
...  

An experiment was conducted on broiler chicks (n=120), reared in cages on a standard diet up to 14th day of age and thereafter up to 42nd day (14-42d) on test diets with or without MOS. The 14th day old chicks were randomly distributed into three dietary treatment groups viz., T1 (control diet), T2 (control diet with MOS @ 0.3%) and T3 (control diet with MOS @ 0.5%). Each dietary treatment comprised of four replicates of eight birds each. Experiment was carried out during hot-dry summer. It was found that production indices improved (P>0.001) significantly during all phases. Cellular and humoral immunity at 28th day of age improved significantly (P>0.05) due to MOS supplementation. The percentage of hemoglobin, protein, AST and ALT increased significantly (P>0.001) in MOS supplemented group at 28th as well as 42nd day of age. While H:L ratio, serum corticosterone and cholesterol levels decreased significantly (P>0.001) in MOS supplemented groups. Supplementation of MOS at the both levels caused significant (P>0.001) down regulation of HSP70 expression in jejunum tissues during 28th and 42nd day. Based on this study it was concluded that supplementation of 0.3% MOS in broiler diets significantly improved their performance and welfare during heat stressed conditions.

Author(s):  
J.J. Rokade ◽  
S.K. Bhanja ◽  
A.S. Shinde ◽  
Sajjad, Darshana ◽  
B. Bhaisare ◽  
...  

The present study was undertaken to evaluate the efficacy of aspirin as anti-heat stressor. Broiler chicks were reared on a standard diet up to 14 days of age. Thereafter, the chicks were randomly distributed into three dietary treatment groups viz., T1 (Control group: Standard diet), T2 (Standard diet with aspirin@250 mg/kg) and T3 (Standard diet with aspirin@500 mg/kg). Each treatment was having five replicates of eight birds per replicate. Experiment was carried out during hot-dry (April-May, 30.0±0.70 to 37.0±1.40C, Rh, %: 58.05±1.32 to 70.11±0.82) summer. Feed intake and live weight gain was comparable among the treatments but FCR, protein and energy efficiency improved significantly (P less than 0.001) on aspirin supplementation. The relative yield of thymus and bursa at 4th and 6th week of age improved significantly (P less than 0.05). The percentage of protein, aspartate transaminase (AST) and alanine transaminase (ALT) increased significantly (P less than 0.001) due to aspirin supplementation at 4th as well as 6th week of age. While H:L ratio, serum corticosteron and serum cholesterol (only at 42nd day of age) decreased significantly (P less than 0.001) in aspirin supplemented groups at 28th as well as 42nd day of age. Supplementation of aspirin at 500 mg/kg reduced relative expression of HSP70 in jejunum tissues during 28th or 42nd day of age. It can be concluded that aspirin supplementation 500 mg/kg in diet of heat stressed broilers improved performance as well as welfare..


2021 ◽  
Author(s):  
Olugbenga David Oloruntola

Abstract This study looked at the effects of processed kola nut husks meal (PKHM) utilization as a feed ingredient on broiler chicken in a 42-day feeding trial. Kola nut pod husks were processed into a PKHM using ash treatment and rumen liquor fermentation. Three experimental diets were developed at both the starter and finisher phases, with PKHM included at 0, 4, and 8%, and dubbed diets 1, 2, and 3, respectively. In a fully randomised design, 240 Arbor Acres broiler chicks were randomly assigned to three treatments (10 birds per replicate). Except for the significantly improved (P<0.05) feed conversion ratio of broiler chickens fed diets 2 and 3 at the grower phase (22-42 days) and overall (0-42 days), the performance indices were not significantly (P>0.05) affected by PKHM dietary inclusion. Broiler chicken carcass characteristics and relative internal organ weights remained constant (P>0.05) through diets. The serum glutathione concentration in broiler chickens fed an 8 percent PKHM inclusive diet increased significantly (P<0.05) than those on the control diet and 4 percent PKHM inclusive diet. When broiler chickens fed an 8 percent PKHM inclusive diet were compared to those fed a control diet, the serum catalase concentration was significantly higher (P<0.05). The total serum protein, creatinine, alanine aminotransferase, aspartate transferase, and cholesterol levels remained constant (P>0.05) regardless of dietary treatment. Dietary PKHM inclusion of up to 8% enhanced improved feed efficiency and increased antioxidant enzyme concentration and did not affect the serum biochemical indices concentration.Statement of NoveltyKola nut husk is one of the agro-wastes that contributes to environmental deterioration due to its underutilization. The kola nut husk is underutilised in broiler production due to its chemical constitution, minimal nutritional value, and poor digestion. In this research, kola nut husk meal was ash treated and rumen liquor fermented before being integrated at varying levels in the experimental diets and the performance characteristics, antioxidant status, serum protein, enzymes and biochemical were evaluated. The findings of this study could aid in the development of an agro-waste-based, low-cost functional feed for broiler chicks that incorporates the underutilised kola nut husk and other abundant agrowastes as macro-ingredients.


Author(s):  
A. W. Lakkawar ◽  
M. L. Sathyanarayana ◽  
H. D. Narayanaswamy ◽  
SPACE Sugunarao ◽  
S. Yathiraj ◽  
...  

The ability of Diatomacious earth (DAE) in reducing the toxic effects of aflatoxin (AF) in broiler diet was evaluated. DAE was supplemented @ 400 and 800 mg kg-1 of feed along with 0.5 and 1 ppm of AF kg-1 of feed. Healthy unsexed day old broiler chicks (n=360) were assigned to 9 groups comprising of control and treatment groups. Feeding of AF resulted in significantly lower feed intake as well as body weight gain and increase in feed conversion ratio in comparison to the control groups. Supplementation of DAE in aflatoxin mixed diet significantly reduced the deleterious effects of AF on growth parameters. Feeding of AF alone caused significant decrease in serum levels of total proteins, albumin, triglycerides and cholesterol. However, significant increase was found in serum levels of AST, ALT, ALP, BUN and creatinine in AF fed broilers. Supplementation of DAE to the AF mixed feed ameliorated the adverse effect of AF on the serum biochemical values by causing increase in serum total proteins, albumin, triglycerides and cholesterol levels. In addition, a significant increase was recorded in AST, ALT, ALP, BUN and creatinine levels in DAE supplemented broilers. The study concluded that diatomaceous earth is an effective adsorbent to decrease the negative effects of aflatoxin in broiler chicken.


2021 ◽  
Vol 38 (2) ◽  
pp. 97-106
Author(s):  
A. O. Lala ◽  
A. O. Oso ◽  
A. O. Fafiolu ◽  
A. M. Bamigbose ◽  
D. Eruvbetine ◽  
...  

The effects of instant noodles waste (INW) as a replacement for maize was investigated using 144 day-old, unsexed broiler chicks of Anak strain which were randomly assigned into four dietary treatment groups of 36 birds each. Each dietary treatment group had 3 replicates of 12 broilers per replicate. These four different diets were fed, in which INW replaced maize at 0% (control), 10, 20 and 30% from day old to 8 weeks of age. The growth performance, nutrient digestibility, haematological parameters and carcass traits of the chickens were evaluated. Average final live from 1559.44 g to 1730.91g with broilers fed control diet and 30% INW diet recording the highest and least values respectively among the treatments. Broilers fed the 30 % INW had lower (P< 0.05) live weight. Broilers fed INW based diets had higher blood profile comparable to the control. The results indicated that INW can replace up to 20% of broiler starter diet and 30% replacement of maize at the finisher phase without adverse effects on growth performance of the broilers.  


2020 ◽  
Vol 36 (3) ◽  
pp. 317-327
Author(s):  
Haruna Abimiku ◽  
Comfort Tuleun ◽  
Oluwabiyi Oluremi ◽  
Elijah Faith

A feeding trial was conducted with two hundred (200) Arbor Acre strain of broiler chicks to determine the effect of soybean residue (SBR) on carcass characteristics and visceral organs. Birds were fed for 28 days (starter phase) with formulated diet containing 22% crude protein and 3000Kcal/kg (ME). Five iso-nitrogenous experimental diets were formulated which contain SBR. In the control diet (T1) groundnut cake was served as the only protein source. Four (4) test diets designated as: T2, T3, T4 and T5 were formulated with SBR replacing 25, 50, 75 and 100% of groundnut cake respectively in finisher phase. The birds were randomly grouped into five (5) experimental treatment groups in four (4) replicates of 40 birds per treatment in a completely randomized design. The finisher phase lasted for five (5) weeks and the birds were fed and given drinking water adlibitum. All carcass characteristics evaluated differ significantly (P<0.05) among treatment groups except breast and wings. No significant (P>0.05) difference were shown for organs, while shanks was only the residue that was significantly affected by dietary treatment. Soybean residue can be used up to 100% to replace groundnut cake in the diet of broiler chickens thus providing a productive use for this hither to neglected agro allied waste.


2021 ◽  
Vol 4 (1) ◽  
pp. 47-53
Author(s):  
Nwe Nwe Htin ◽  
May Phyo Chit

A completely randomized design (CRD) was used with a total of 160 day-old commercial broiler chicks which were randomly assigned to four treatment groups with five replicates into 20 pens and brooded. Feed and water were provided ad libitum. From day old chick to 21 day, the broiler chicks were fed Diet 1 (5% fish meal and 25% soybean meal, 1: 5), Diet 2 (3% fish meal and 30% soybean meal, 1: 10) was served as control diet, Diet 3 (2% fish meal and 30% soybean meal, 1: 15) and Diet 4 (1.5% fish meal and 30% soybean meal, 1: 20). From day 22 onwards, the broiler chicks were fed finisher diet, Diet 1 (5% fish meal and 25% soybean meal, 1: 5), Diet 2 (2.6% fish meal and 26% soybean meal, 1: 10, serve as control diet, Diet 3 (1.8% fish meal and 27% soybean meal, 1: 15) and Diet 4 (1.3% fish meal and 26% soybean meal, 1: 20). Weekly performances and mortality are measured. By feeding FS5 diet (5% fish meal and 25% soybean meal), the heavier body weight, increased feed consumption and feed conversion ratio of broiler chicks was observed when compared to that of other treatment groups. Conclusively, it is obvious that the ratio of dietary fish meal to soybean meal 1: 5 (FS5) improved body weight but the lower feed efficiency of this diet should be considered in economical point of view.


2021 ◽  
Vol 8 ◽  
Author(s):  
Lily P. Hernandez ◽  
James L. Dunn ◽  
Joel Wenninghoff ◽  
Amanda Hesse ◽  
Crystal L. Levesque

Times of high metabolic activity in gestation and lactation, as well as periods of stress at weaning, can lead to greater incidences of oxidative stress in the dam and offspring during the suckling and postweaning period. Oxidative stress is an imbalance between prooxidant molecules and the antioxidant defense system that can negatively impact growth and/or reproductive performance. The objective of this research was to evaluate the effectiveness of whole yeast cell, peppermint oil, and ɤ-tocopherol in gestation and lactation on maternal oxidative status and offspring growth from birth to market. In study 1, 45 sows and gilts were assigned to one of four diets [control diet (CON), control + whole yeast cell (YC), control + mint oil top dress (MO), and control + yeast cell and mint oil top dress (YCMO)] provided from d110 of gestation through to weaning. A total of 481 weaned offspring were randomly allotted to pens balanced by weight and litter within maternal treatment and received the same dietary treatment as the sow for 35 days postwean in a four-phase feeding regimen. In study 2, 53 sows and gilts were allotted to four diet regimens similar to study 1 [CON, YC, MO, and control + ɤ-tocopherol (GT)] from d5 postbreeding to weaning. At weaning, 605 piglets were randomly allotted to pens, balanced by weight and litter within maternal treatment and fed a common diet for 126 days postwean in a nine-phase feeding regimen. Maternal dietary treatment did not impact sow body weight, piglet birth weight, and litter size in either study. In study 1, piglets from YC sows were heavier (p &lt; 0.05) at weaning than CON animals. In the postwean period, overall daily gain was greater (p &lt; 0.05) for CON-fed pigs than YCMO pigs, with overall feed intake greater (p &lt; 0.05) for YCMO- than MO-fed pigs, resulting in lower (p &lt; 0.05) Gain to Feed (G:F) in YCMO-fed pigs. In study 1, glutathione content in milk tended to be lower (p &lt; 0.10) in MO than in YCMO sows. In study 2, piglets from GT-fed sows tended to be heavier (p &lt; 0.10) at weaning than YC piglets. Lightweight pigs from CON sows tended to be lighter (p &lt; 0.10) than pigs from all other treatment groups at weaning and day (d) 29 postwean. Lightweight MO and GT pigs were heavier at d42 (p &lt; 0.05) than CON and YC pigs. At d70 postwean, GT pigs tended to be heavier than CON pigs. Lightweight MO pigs had greater gain (p &lt; 0.05) during the finishing period than all other treatment groups. With respect to sow oxidative status in study 2, glutathione content in colostrum and d4 and 14 milk samples did not differ by maternal treatment. Superoxide dismutase activity in sow sera, colostrum, and milk did not differ between diets in either study. Whole yeast cell and ɤ-tocopherol supplementation in sow lactation diets resulted in heavier offspring. However, pre- and postnatal exposure to mint oil benefited lightweight pigs up to market weight.


2021 ◽  
Vol 30 (2) ◽  
pp. 169-178
Author(s):  
B. M. Oruwari ◽  
A. O. Anibo ◽  
D. M. Nkanta

A feeding trial using 240, day old Anak broiler chicks was conducted in which cassava meal (CM) completely replaced maize in the control diet (A) to formulate five isocaloric and isonitrogenous diets. Dried CM blended with brewers dried yeast (BDY) formed cassayeast with 2.704 parts of CM to 1 part of BDY. The (Cassayeast) CM and BDY blend in the dietary treatments, (B,C,D and E) were 30 CM + 11.1% BDY, 35 CM +12.94% BDY, 40 CM + 14.79% BDY, and 45 CM + 16.64% BDY, respectively, and these treatments had no maize. Dietary treatment A, containing maize, which served as the control had no cassayeast inclusion, thus demonstrating the total replacement of maize by cassayeast in the other dietary treatments. Results showed that significant of maize difference (P<0.5) were observed in the control and at in one cassayeast treatment in feed intake (4.43 as against 4.22kg), body weight (1.94 as against 1.65kg), efficiency of feed utilization (feed: gain ratio 2.19 as against 2.51; gain: feed ratio 0.49 as against 0.43), respectively. However, gizzard weights were not significant. Economic analysis showed that the cost of producing a bird with the maize based diet (A) was significantly (P<0.05) higher than that with the cassayeast diets but there was not difference among the cassayeast diets (B-E). The maize based diet was most expensive (₦ 29.68 per kg diet) while diet C was the cheapest (₦ 25.57 per kg). The study demonstrated that the use of cassaveast has a place in the broiler industry, thus indicating that with proper protein balancing, cassava can completely replace maize in broiler diets.


1997 ◽  
Vol 48 (2) ◽  
pp. 147 ◽  
Author(s):  
G. M. Smith ◽  
C. L. White

We determined the effects of increased dietary concentrations of molybdenum and sulfur on the accumulation and tissue concentrations of cadmium in sheep, and compared them with effects on copper. Forty sheep, each weighing approximately 40 kg, were adjusted for 3 weeks to a basal diet of 80% wheaten chaff and 20% lupin seed containing (per kg dry weight) 0·016 mg Cd, 0·45 mg Mo, 3·4 mg Cu, and 1·9 g S. On Day 0 of treatment, 8 sheep were killed and the tissues analysed for trace minerals to provide a baseline value. The remaining sheep were divided into 4 dietary treatment groups: control (basal diet plus 4 mg Cd/kg), +Mo (control diet plus 15 mg Mo/kg), +S (control diet plus 4 g S/kg), +Mo+S (control diet+15 mg Mo+4 g S/kg). The treatment period lasted 80 days, after which sheep were killed for tissue samples. Sulfur alone reduced the accumulation of Cd in liver, kidney, and muscle by 60% compared with control sheep (P < 0·05). Molybdenum alone reduced Cd accumulation by 40% in liver and muscle (P < 0·05) and 30% in kidney (P = 0·09). When provided together (+Mo+S), the effect was equivalent to feeding with Mo alone, showing that Mo blocked the effect of S. Cadmium concentrations in whole kidneys for the 4 respective treatments were 6·40 ± 0· 7, 2·8 ± 0·3, 4·5 ± 0·8, and 5·0 ± 0·5 mg/kg fresh weight. The pre-treatment concentration was 0·7 ± 0·2 mg/kg. For Cu in blood and tissues, the effects of Mo and S treatment were consistent with the thiomolybdate hypothesis, and were quite different from those seen for Cd. Copper concentrations in whole kidney for the 4 treatments were 4·1 ± 0·1, 3·5 ± 0·2, 4·7 ± 0·3, and 22·4 ± 3·9 mg/kg fresh weight. The pre-treatment concentration was 4·1 ± 0·3 mg/kg. The results show that increased dietary levels of Mo and S reduce the accumulation of Cd in tissues, and the mechanisms of action differ from those involving Cu.


Foods ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 320 ◽  
Author(s):  
Besma Omri ◽  
Nadir Alloui ◽  
Alessandra Durazzo ◽  
Massimo Lucarini ◽  
Alessandra Aiello ◽  
...  

This study evaluated the effect of dietary incorporation of linseed alone or along with dried tomato paste-pepper powder mix on egg physical characteristics, antioxidant profiles, lipid oxidative status, and yolk coloration before and after storage at 4 °C for one month. Sixty Novogen White laying hens, 27 weeks-old, were divided into three groups and given 100 g/hen/day of a standard diet (C), standard diet containing 4.5% of ground linseed (L), linseed diet containing 1% of dried tomato paste and 1% of sweet red pepper (LTP). Linseeds increased (p < 0.05) egg yolk antioxidant capacity but not lipid oxidative stability (p > 0.05). However, dietary inclusion of LTP did not improve fresh egg yolk antioxidant activity and lipid oxidation stability (p > 0.05). With reference to the stored eggs, only antioxidant activity measured by phosphomolybdenum reduction and lipid oxidative stability were influenced (p < 0.05) by the dietary treatment. Fresh egg yolk of hens fed on linseeds tended to have a slightly more yellow, redder, and less light color than the eggs of hens fed with the control diet. Dietary supplementation of LTP increased (p < 0.05) the Roche yolk color fan (RYCF) score and redness (a*) and decreased (p < 0.05) lightness (L*) without affecting (p > 0.05) saturation (C*). Storage of hens’ eggs fed on the control diet did not influence (p > 0.05) yolk color.


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