Study of beet bagasse effect on biochemical characteristics, photosynthetic pigments and antioxidant activity of leaf extracts of peppermint (Mentha piperita L.) under drought stress conditions

Abstract Background Abiotic and biotic stresses induce oxidative processes in plant cells that this process starts with the production of ROSs which cause damage to the proteins. Therefore, plants have increased their antioxidant activity to defend against this oxidative stress to be able to handle stress better. In this research, 14 different tea accessions in a randomized complete block design with two replications were evaluated in two normal and drought stress conditions, and their antioxidant activity was measured by DPPH-free radicals’ assay and gene expression analysis. Results The results of gene expression analysis showed that the 100 and 399 accessions and Bazri cultivar had high values for most of the antioxidant enzymes, ascorbate peroxidase, superoxide dismutase, catalase, and peroxidase under drought stress conditions while the 278 and 276 accessions had the lowest amount of antioxidant enzymes in the same situation. Results showed that the IC50 of the BHT combination was 90.12 μg/ ml. Also, The IC50 of accessions ranged from 218 to 261 μg/ml and 201–264 μg/ml at normal and drought stress conditions, respectively. The 100 and 399 accessions showed the lowest IC50 under normal and drought stress conditions, while 278 and 276 accessions had the highest value for IC50. The antioxidant activity of tea accession extracts under normal conditions was ranged from 25 to 69% for accessions 278 and 100, respectively. While, the antioxidant activities of extracts under drought stress condition was 12 to 83% for accessions 276 and 100, respectively. So, according to the results, 100 and 399 accessions exhibited the least IC50 and more antioxidant activity under drought stress conditions and were identified as stress-tolerant accessions. However, 278 and 276 accessions did not show much antioxidant activity and were recognized as sensitive accessions under drought stress conditions. Conclusions These results demonstrate that total phenol content, antioxidant activity, and the oxygen-scavenging system can be used as a descriptor for identifying drought-tolerant accessions.

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COMPARATIVE EVALUATION OF ANTIOXIDANT ACTIVITY IN SEVERAL PERENNIAL ONION

VEGETABLE CROPS OF RUSSIA ◽

10.18619/2072-9146-2018-5-73-76 ◽

2018 ◽

pp. 73-76

Author(s):

N. A. Golubkina ◽

T. M. Seredin ◽

A. V. Molchaniva ◽

O. V. Kosheleva

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Vitamin C ◽

Photosynthetic Pigments ◽

Comparative Evaluation ◽

Water Soluble ◽

Soluble Solids ◽

Ascorbic Acid Concentration ◽

Allium Species ◽

Biochemical Characteristics

Evaluation of biochemical characteristics of 8 perennial Allium species is achieved (A. ramosum, A. caeruleum, A. erubescens, A. obliquum, A. moly, A. aflatunense, A. oreophilum and A.ursinum). High nutritional significance of separate decorative species is demonstrated. A. caeruleum is shown to be a leader in the accumulation of ascorbic acid (more than 9000 mg/100 g d.w.) and polyphenols (more than 8000 mg GAE/kg d.v.). Detected ascorbic acid concentration range for 8 studies Allium species was 568 (A. ramosum) – 9980 (A. caerulum) mg/100 g d.w.; polyphenols 1392 (A. obliquum) – 8582 (A. caerulum) mg GAE/kg d.w.; water-soluble compounds 28 (A. aflatunense, A. obliguum) – 69 (A. ramosum) mg/kg d.w.; chlorophyll 0.54 (A.oreophilum) – 1.69 (A. ursinum) mg/100 g d.w.; carotene 0.07 (A. oreophilum) – 0.25 (A.ursinum) mg/100 g d.w.; selenium 72 (A. ursinum) – 245 μg/kg d.w., (A. ramosum). Adequate consumption level of vitamin C may be provides by 6.2 g of A.caerulum leaves. Antioxidant activity of Allium species alcoholic extracts composed a range from 1.5 (A. obliquum) to 6.4 (A. ursinum) mg GAE/g d.w. A. ramosum and A. oreophilum demonstrated the highest content of total soluble solids. The highest content of photosynthetic pigments happened to be typical for A. ursinum. The ratio between antioxidant activity of alcoholic and water extracts of Allium leaves was in the range between 0.89 and 2.21 depending on plant species: the highest value was registered in leaves of A. ursinum, the lowest – in leaves of A. ramosum. In a whole the highest content of antioxidants was demonstrated for leaves of A. caerulum. The highest levels of photosynthetic pigments and antioxidant activity of alcoholic extracts were indicated in leaves of A. ursinum.

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Ameliorative effect of melatonin improves drought tolerance by regulating growth, photosynthetic traits and leaf ultrastructure of maize seedlings

BMC Plant Biology ◽

10.1186/s12870-021-03160-w ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Shakeel Ahmad ◽

Ihsan Muhammad ◽

Guo Yun Wang ◽

Muhammad Zeeshan ◽

Li Yang ◽

...

Keyword(s):

Drought Stress ◽

Plant Growth ◽

Photosynthetic Pigments ◽

Field Capacity ◽

Maize Seedling ◽

Stress Conditions ◽

Antioxidant Enzyme Activities ◽

Plant Tolerance ◽

Maize Seedlings ◽

The Impact

Abstract Background Melatonin is considered a potential plant growth regulator to enhance the growth of plants and increase tolerance to various abiotic stresses. Nevertheless, melatonin’s role in mediating stress response in different plant species and growth cycles still needs to be explored. This study was conducted to understand the impact of different melatonin concentrations (0, 50, 100, and 150 μM) applied as a soil drench to maize seedling under drought stress conditions. A decreased irrigation approach based on watering was exposed to maize seedling after drought stress was applied at 40–45% of field capacity. Results The results showed that drought stress negatively affected the growth behavior of maize seedlings, such as reduced biomass accumulation, decreased photosynthetic pigments, and enhanced the malondialdehyde and reactive oxygen species (ROS). However, melatonin application enhanced plant growth; alleviated ROS-induced oxidative damages by increasing the photosynthetic pigments, antioxidant enzyme activities, relative water content, and osmo-protectants of maize seedlings. Conclusions Melatonin treatment also enhanced the stomatal traits, such as stomatal length, width, area, and the number of pores under drought stress conditions. Our data suggested that 100 μM melatonin application as soil drenching could provide a valuable foundation for improving plant tolerance to drought stress conditions.

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Photosynthetic pigments and photosynthetic products of endophyte-infected and endophyte-free Lolium perenne L. under drought stress conditions

Frontiers of Biology in China ◽

10.1007/s11515-006-0025-8 ◽

2006 ◽

Vol 1 (2) ◽

pp. 168-173 ◽

Cited By ~ 5

Author(s):

Anzhi Ren ◽

Yubao Gao ◽

Wei Wang ◽

Jinlong Wang

Keyword(s):

Drought Stress ◽

Lolium Perenne ◽

Photosynthetic Pigments ◽

Stress Conditions ◽

Lolium Perenne L ◽

Photosynthetic Products

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Co-application of bio-fertilizer and salicylic acid improves growth, photosynthetic pigments and stress tolerance in wheat under drought stress

PeerJ ◽

10.7717/peerj.9960 ◽

2020 ◽

Vol 8 ◽

pp. e9960

Author(s):

Ammar Azmat ◽

Humaira Yasmin ◽

Muhammad Nadeem Hassan ◽

Asia Nosheen ◽

Rabia Naz ◽

...

Keyword(s):

Salicylic Acid ◽

Drought Stress ◽

Drought Tolerance ◽

Photosynthetic Pigments ◽

Foliar Application ◽

Combined Treatment ◽

Foliar Spray ◽

Stress Conditions ◽

Wheat Crop ◽

Combined Application

Drought stress hampers the growth and productivity of wheat crop worldwide. Thus far, different strategies have been proposed to improve drought tolerance in wheat but the combined application of plant growth-promoting rhizobacteria formulated bio-fertilizer (BF) and salicylic acid (SA) has not been thoroughly explored yet. Therefore, a pot experiment was conducted to observe the effect of SA, BF, and their combination on wheat plants under optimal and drought stress conditions. Seeds priming was done with BF (107 CFU mL−1). After 2 weeks of germination, SA (one mM) was applied as a foliar spray. Drought stress was applied by withholding water supply at three-leaf stage (30 d old plants) for the next 15 d until soil moisture dropped to 10%. Foliar application of SA increased the bacterial population of BF significantly compared to the sole application of BF under irrigated as well as drought stress conditions. Co-application of BF and foliar spray of SA induced drought tolerance in wheat plants by enhancing plant biomass, photosynthetic pigments, relative water content and osmolytes, and activities of the defense-related system. Plants treated with SA and BF together under drought stress had significantly increased leaf water status, Chl a, Chl b, and carotenoids synthesis by 238%, 125%, 167%, and 122%, respectively. Moreover, the co-application of SA and BF showed maximum SOD, POD, APX, and CAT activities by 165%, 85%, 156%, and 169% in the leaves while 153%, 86%, 116% and 200% in roots under drought stress. Similarly, the combined treatment exhibited a pronounced decrease in MDA content by 54% while increased production of proteins and proline by 145% and 149%, respectively. Our results showed that the co-application of SA and BF induced better drought tolerance as compared with the sole application of SA or BF. The results obtained herein suggest that combined application of BF and SA can be applied to the wheat crop to greatly improve drought tolerance in field conditions.

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Evaluation of Some Biochemical Characteristics of Some Red Bean Ecotypes under Drought Stress Conditions

Journal of Crop Breeding ◽

10.29252/jcb.11.29.55 ◽

2019 ◽

Vol 11 (29) ◽

pp. 55-64

Author(s):

Amin Baghizadeh ◽

Somayeh Mohammadinejad ◽

Mehdi Rahimi ◽

◽

...

Keyword(s):

Drought Stress ◽

Stress Conditions ◽

Biochemical Characteristics ◽

Red Bean

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Phytochemical and antioxidant analysis of medicinal and food plants towards bioactive food and pharmaceutical resources

Scientific Reports ◽

10.1038/s41598-021-89437-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Manyou Yu ◽

Irene Gouvinhas ◽

João Rocha ◽

Ana I. R. N. A. Barros

Keyword(s):

Antioxidant Activity ◽

Gallic Acid ◽

Dry Weight ◽

The Other ◽

Bioactive Molecules ◽

Food Plants ◽

Mentha Piperita ◽

Petroselinum Crispum ◽

Leaf Extracts ◽

Pharmaceutical Industries

AbstractPlants with medicinal properties play an increasingly important role in food and pharmaceutical industries for their functions on disease prevention and treatment. This study characterizes the phenolic composition and antioxidant activity of seven medicinal and food plants, including the leaves of Salvia officinalis L., Rosmarinus officinalis L., Olea europaea L., and Punica granatum L., as well as the leaves and young stems of Ruta graveolens L., Mentha piperita L., and Petroselinum crispum, Mill., by using colorimetric, chromatographic, and spectrophotometric assays. Results revealed that the hydro-methanolic leaf extracts of P. granatum (pomegranate) displayed the highest content of total phenols (199.26 mg gallic acid per gram of plant dry weight), ortho-diphenols (391.76 mg gallic acid per gram of plant dry weight), and tannins (99.20 mg epicatechin per gram of plant dry weight), besides a higher content of flavonoids (24 mg catechin per gram of plant dry weight). The highest antioxidant capacity measured by ABTS, DPPH, and FRAP (2.14, 2.27, and 2.33 mM Trolox per gram of plant dry weight, respectively) methods was also obtained in pomegranate leaf extracts, being 4–200 times higher than the other species. Such potent antioxidant activity of pomegranate leaves can be ascribed to the presence of different types of phenolic compounds and the high content in tannins, whilst phenolic acids and flavonoids were found to be the dominant phenolic classes of the other six plants. Consequently, despite the well-known antioxidant properties of these plant species, our study suggests pomegranate leaf can stand out as a relatively more valuable plant source of natural bioactive molecules for developing novel functional food-pharma ingredients, with potential for not only promoting human health but also improving bio-valorization and environment.

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