Stress responses of transportation on red tilapia which given feed containing chromium

ABSTRACTThis study was conducted to evaluate stress responses of transportation on red tilapia Oreochromis sp. which given feed containing chromium. Three isonitrogenous and isocaloric experimental feeds were prepared, these diets were control (without chromium), CrPic 1 mg/kg, and CrYst 2 mg/kg supplementation in feed, all group were arranged triplicate. Satiation feeding was done three times a day. After a 60-day feeding experiment, the experimental fishes were fasted and distributed in polyethylene bags (N=60 fish/bag) containing 3 L of water, subjected to condition of transport simulation for 13 hours. Survival rate, levels of plasma cortisol, blood glucose, superoxide dismutase (SOD), and malondialdehyde (MDA) enzyme were observed at before transportation, after transportation, one day, and two days after transportation. The result showed that chromium supplementation reduced the levels of plasma cortisol before and after transportation, one day, and two days after transportation. Also, it decreased blood glucose compared with control significantly before transportation and one day after transportation. The SOD enzyme concentration increased significantly after fish was fed with feed containing chromium for 30 days, while the MDA enzyme concentration increased significantly after two days of transportation. However, there was no significant difference in the survival of red tilapia between treatments. The best result was obtained in the treatment of fish which fed with feed containing chromium. CrPic supplementation 1 mg/kg and CrYst 2 mg/kg increased the body resistance in red tilapia by decreasing the negative effect of stress while transportation. Keywords: stress, transportation, red tilapia, chromium ABSTRAKPenelitian dilakukan untuk mengevaluasi respons stres transportasi ikan nila merah Oreochromis sp. yang diberikan pakan yang mengandung kromium. Pada penelitian ini digunakan tiga jenis pakan, terdiri atas pakan tanpa suplementasi kromium (kontrol), pakan bersuplementasi kromium pikolinat (CrPic 1 mg/kg), dan kromium yeast (CrYst 2 mg/kg), semua perlakuan diulang sebanyak tiga ulangan. Pemberian pakan sebanyak tiga kali sehari dan dilakukan secara at satiation. Setelah 30 hari pemeliharaan, ikan uji dipuasakan dan didistribusikan dalam plastik polietilen (N=60 ekor ikan/kantong plastik) yang berisi 3 L air, dilakukan dengan simulasi transportasi selama 13 jam. Parameter yang diamati pada penelitian ini adalah kelangsungan hidup, kortisol, glukosa darah, enzim superoksida dismustase (SOD), dan malondialdehida (MDA) saat sebelum transportasi, sesaat setelah transportasi, sehari, dan dua hari setelah transportasi. Hasil yang didapatkan adalah suplementasi kromium menurunkan konsentrasi kortisol secara signifikan sebelum transportasi, sesaat, sehari, dan dua hari setelah transportasi. Suplementasi kromium menurunkan glukosa darah secara signifikan pada saat sebelum transportasi dan sehari setelah transportasi. Konsentrasi enzim SOD meningkat secara signifikan setelah pemberian pakan bersuplementasi kromium selama 30 hari, sedangkan konsentrasi enzim MDA meningkat secara signifikan setelah dua hari transportasi pada ikan yang diberi pakan bersuplementasi kromium. Namun, tidak ada perbedaan yang signifikan pada kelangsungan hidup ikan nila merah antarperlakuan. Hasil terbaik diperoleh pada perlakuan ikan dengan suplementasi kromium. Suplementasi 1 mg/kg CrPic dan 2 mg/kg CrYst dapat meningkatkan daya tahan tubuh pada budidaya ikan nila merah dengan menurunkan pengaruh negatif stres akibat transportasi. Kata kunci: stres, transportasi, nila merah, kromium

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Konseling Gizi, Kualitas Diit Berbasis Pangan Lokal Dengan Kadar Glukosa Darah Penderita Diabetes Mellitus Tipe Ii Di Wilayah Kerja Puskesmas Bahu

Jurnal GIZIDO ◽

10.47718/gizi.v10i2.744 ◽

2018 ◽

Vol 10 (2) ◽

pp. 108-112

Author(s):

Rivolta G,M, Walalangi ◽

Muksin Pasambuna ◽

Rudolf B. Purba ◽

Agung Suryohadi

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Local Food ◽

T Test ◽

The Body ◽

Significance Level ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Significant Difference ◽

Before And After

Diabetes Mellitus is a group of chronic disease which is characterized by increased levels of glucose in the blood due to disorders of the body's metabolism system, in which the pancreas organ incapable of producing the hormone insulin according to needs of the body. Nutritional counselling with quality local food based diit can help control blood glucose levels. This research aims to identify the difference in blood glucose levels and the value of quality diit type II diabetes mellitus patients before and after given nutritional counseling with quality local food based diit. This type of research is Pre Experimental research using one group pretest-posttest design. The number of samples is determined by purposive sampling counted 31 people. Data analysis using Wilcoxon and T-test analysis. The Wilcoxon analysis with significance level of 95% can be seen to produce significant difference in pre-post glycemic index component with p=0,000 (p<0,05) and diversity of pre-post food with p=0,002 (p<0,05). In the result of T-test for pre-post blood glucose variable, there was no significant difference p=0,105 (p>0,05). However, there was a range of decreased glucose levels before and after in 22 respondents with an average decrease of 11.42 mg/dL. Conclusion there was no difference of blood glucose level before and after nutrition counseling with quality of diet based on local food.

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The influence of osteopathic correction on the rate of various rhythms of the body

Russian Osteopathic Journal ◽

10.32885/2220-0975-2019-1-2-64-71 ◽

2019 ◽

pp. 64-71 ◽

Cited By ~ 1

Author(s):

A. E. Chernikova ◽

Yu. P. Potekhina

Keyword(s):

Heart Rate ◽

Respiratory Rate ◽

Age Groups ◽

Biomechanical Properties ◽

Dispersion Analysis ◽

The Body ◽

Body Tissues ◽

Age Related ◽

Significant Difference ◽

Before And After

Introduction. An osteopathic examination determines the rate, the amplitude and the strength of the main rhythms (cardiac, respiratory and cranial). However, there are relatively few studies in the available literature dedicated to the influence of osteopathic correction (OC) on the characteristics of these rhythms.Goal of research — to study the influence of OC on the rate characteristics of various rhythms of the human body.Materials and methods. 88 adult osteopathic patients aged from 18 to 81 years were examined, among them 30 men and 58 women. All patients received general osteopathic examination. The rate of the cranial rhythm (RCR), respiratory rate (RR) heart rate (HR), the mobility of the nervous processes (MNP) and the connective tissue mobility (CTM) were assessed before and after the OC session.Results. Since age varied greatly in the examined group, a correlation analysis of age-related changes of the assessed rhythms was carried out. Only the CTM correlated with age (r=–0,28; p<0,05) in a statistically significant way. The rank dispersion analysis of Kruskal–Wallis also showed statistically significant difference in this indicator in different age groups (p=0,043). With the increase of years, the CTM decreases gradually. After the OC, the CTM, increased in a statistically significant way (p<0,0001). The RCR varied from 5 to 12 cycles/min in the examined group, which corresponded to the norm. After the OC, the RCR has increased in a statistically significant way (p<0,0001), the MNP has also increased (p<0,0001). The initial heart rate in the subjects varied from 56 to 94 beats/min, and in 15 % it exceeded the norm. After the OC the heart rate corresponded to the norm in all patients. The heart rate and the respiratory rate significantly decreased after the OC (р<0,0001).Conclusion. The described biorhythm changes after the OC session may be indicative of the improvement of the nervous regulation, of the normalization of the autonomic balance, of the improvement of the biomechanical properties of body tissues and of the increase of their mobility. The assessed parameters can be measured quickly without any additional equipment and can be used in order to study the results of the OC.

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Differences in triglyceride levels before and after whey protein intervention in field workers exposed and unexposed to arsenic

JURNAL GIZI INDONESIA ◽

10.14710/jgi.9.2.166-171 ◽

2021 ◽

Vol 9 (2) ◽

pp. 166-171

Author(s):

Eka Putri Rahmadhani ◽

Ani Margawati ◽

Apoina Kartini

Keyword(s):

Whey Protein ◽

Dairy Products ◽

Metabolic Disorders ◽

Fat Metabolism ◽

Exposed Group ◽

The Body ◽

Unexposed Group ◽

Significant Difference ◽

Before And After ◽

Field Workers

Background: A work environment that is exposed to heavy metals, such as a coal mining environment, can change fat metabolism in the body. Changes in fat metabolism will lead to cardiovascular disease. Consumption of dairy products, e.g. whey protein, can reduce the risk of metabolic disorders and cardiovascular disease.Objectives: To test and analyze the differences in triglyceride levels before and after whey protein intervention to field workers with different working conditions.Materials and Methods: This experimental research with pretest and posttest was conducted on field workers at PT Bukit Asam Tbk. Tarahan Port Unit, Lampung as an Arsenic exposed group and PTPN VII Way Berulu Business Unit as an Arsenic unexposed group. Both groups received 24 grams of whey protein daily for 28 days. The triglyceride levels before and after the intervention were measured by laboratory analysis using the enzymatic calorimetry method. The data were analyzed using independent t-test, Mann Whitney test, and Wilcoxon test.Results: The average triglyceride levels increased by 50.48 ± 98.09 mg/dL in the exposed group and 16.78 ± 67.67 mg/dL in the unexposed group. There was a significant difference in triglyceride levels before and after the whey protein intervention in the two groups.Conclusions: The whey protein intervention increased the triglyceride level in the exposed group and decreased it in the unexposed group.

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Aberrant Lighting Causes Anxiety-like Behavior in Mice but Curcumin Ameliorates the Symptoms

Animals ◽

10.3390/ani11092590 ◽

2021 ◽

Vol 11 (9) ◽

pp. 2590

Author(s):

Dhondup Namgyal ◽

Kumari Chandan ◽

Sher Ali ◽

Ajaz Ahmad ◽

Maha J. Hashim ◽

...

Keyword(s):

Body Weight ◽

Research Field ◽

The Body ◽

Laboratory Animals ◽

Artificial Lighting ◽

Lighting Conditions ◽

Daily Food ◽

Significant Difference ◽

Negative Effect ◽

Lighting Systems

In the modern research field, laboratory animals are constantly kept under artificial lighting conditions. However, recent studies have shown the effect of artificial light on animal behavior and metabolism. In the present study on mice, following three weeks of housing in dim light at night (dLAN; 5lux) and complete darkness (DD; 0lux), we monitored the effect on body weight, daily food intake, anxiety-like behavior by employing the open field test, and expression of the period (PER1) gene. We also studied the effect of oral administration of different concentrations of curcumin (50, 100, and 150 mg/kg) for three weeks in the same mice and monitored these parameters. The exposure to dLAN had significantly increased the anxiety-like behavior and body weight possibly through the altered metabolism in mice, whereas exposure to DD caused increased anxiety but no significant difference in weight gain. Moreover, the expression of the PER1 gene involved in sleep was also found to be decreased in the aberrant light conditions (dLAN and DD). Although the treatment of curcumin had no effect on body weight, it ameliorated the anxiety-like behavior possibly by modulating the expression of the PER1 gene. Thus, alteration in the light/dark cycle had a negative effect on laboratory animals on the body weight and emotions of animals. The present study identifies the risk factors associated with artificial lighting systems on the behavior of laboratory animals and the ameliorative effects of curcumin, with a focus on anxiety-like behavior.

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PRE AND POSTPRANDIAL THERMOGRAPHIC PROFILE OF GREEN IGUANAS (IGUANA IGUANA)

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Veterinary Medicine ◽

10.15835/buasvmcn-vm:12345 ◽

2016 ◽

Vol 73 (2) ◽

pp. 425

Author(s):

Simona Rusu ◽

Zdenek Knotek ◽

Radu Lacatus ◽

Ionel Papuc

Keyword(s):

Heat Transfer ◽

Body Temperature ◽

Temperature Difference ◽

The Body ◽

Significant Difference ◽

Before And After ◽

Significant Temperature ◽

Green Iguanas ◽

The Right ◽

Head Temperature

Abstract The body temperature of 10 clinically healthy green iguanas (Iguana iguana) was measured using a thermographic camera (FLIR E6, Flir Systems Sweden) before and after the food was offered. For each animal there were performed a total of 6 measurements (3 before feeding and 3 after the food was offered). The purpose of this experiment was to observe the thermographic pattern of the body before and after the feeding, since herbivore reptiles tend to bask after the feeding to increase the body temperature that will help them afterwards digest the food. The animals were housed in individual vivariums with every animal having a basking spot available. The pictures were taken outside the vivarium in an adjacent room. The animals were handled with gloves and transported in a cardboard box in order to avoid heat transfer between the handler and the iguana that would have produced thermal artefacts. Each individual was placed on a table on a styrofoam slate, again, to avoid the heat transfer between the table and the animal`s body. For each animal a total of 4 pictures were taken (up, front, left and right). The pictures were analysed with the FLIR Tools program that is provided by the manufacturer and 3 temperatures were taken into consideration (the head temperature, body temperature on the right side and body temperature on the left side). The temperatures were compared between them and with the temperature of the vivariums that consisted of the average between the temperature in 3 different spots (basking spot, the feeding bowl site and the coldest spot) measured with an infrared thermometer GM300 (Benetech, China). The temperature of the body was dependent on the vivarium temperature and it was a significant temperature difference between the measurements before the feeding and after the feeding. Also we discovered a significant difference between the head temperature and the body temperature on the left side before the feeding that disappeared after the animals ate. There was also a significant difference between the temperature on right side and on left side of the animals both before and after the feeding. No significant temperature difference was observed between the head and the right side of the body neither before nor after the feeding.

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Assessment of Blood Glucose and Electrolytes during Cardiopulmonary Bypass in Diabetic and Non-Diabetic Patients of Pakistan

Global Journal of Health Science ◽

10.5539/gjhs.v8n9p159 ◽

2016 ◽

Vol 8 (9) ◽

pp. 159

Author(s):

Muhammad Bilal ◽

Abdul Haseeb ◽

Mohammad Hassaan Khan ◽

Akash Khetpal ◽

Muhammad Saad ◽

...

Keyword(s):

Blood Glucose ◽

Cardiopulmonary Bypass ◽

Repeated Measures ◽

Artery Bypass ◽

The Body ◽

Diabetic Patients ◽

Blood Electrolytes ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Significant Difference

INTRODUCTION: Perioperative hyperglycemia has been shown to be related to higher levels of morbidity and mortality in patients on cardiopulmonary bypass (CPB) undergoing coronary artery bypass grafting (CABG), both diabetic and non-diabetic. Blood electrolytes, like sodium, potassium, calcium, and chloride play a very important role in the normal functioning of the body and can lead to a variety of clinical disorders if they become deficient. A minimal number of studies have been conducted on the simultaneous perioperative changes in both blood glucose and electrolyte levels during CPB in Pakistan. Therefore, our aim is to record and compare the changes in blood glucose and electrolyte levels during CPB in diabetic and non-diabetic patients.MATERIALS & METHODS: This was a prospective, observational study conducted on 200 patients who underwent CABG with CPB, from October 2014 to March 2015. The patients were recruited from the Cardiac Surgery Ward, Civil Hospital Karachi after they complied with the inclusion criteria. Repeated-measures analysis of variance (ANOVA) was used to compare the trend of the changes perioperatively for the two groups.RESULTS: There was no significant difference in changes in blood glucose between the two groups (P = 0.62). The only significant difference detected between the two groups was for PaCO2 (P = 0.001). Besides, further analysis revealed insignificant group differences for the trend changes in other blood electrolytes (P > 0.05).CONCLUSION: Our findings highlighted that there is no significant difference in blood electrolytes changes and the increase in blood glucose levels between diabetic and non-diabetic patients.

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KADAR KLORIDA SERUM PADA LATIHAN FISIK INTENSITAS RINGAN MAHASISWA FAKULTAS KEDOKTERAN UNIVERSITAS SAM RATULANGI

Jurnal e-Biomedik ◽

10.35790/ebm.2.1.2014.3709 ◽

2014 ◽

Vol 2 (1) ◽

Author(s):

Gina Irene Ishak

Keyword(s):

Light Intensity ◽

Physical Exercise ◽

Chloride Ion ◽

The Body ◽

Wilcoxon Test ◽

Purposive Sampling ◽

Serum Chloride ◽

Significant Difference ◽

Before And After ◽

Rate Maximum

Abstract: Physical exercise stimulates sweat from inside the body to transfer the heat out of it. Sweat consists of both water and electrolytes, including chloride ion, so if the excretion of water and electrolytes is not corrected can disturb the body’s homeostasis and health. This study aimed to determine the differencesof serum chloride levels before and after light intensity physical exercise. This study was a pre - experimental with one group pretest – posttest design. There were 30 respondents obtained by using purposive sampling method. The respondents did a brisk walk on a treadmill at 50-63 % heart rate maximum intensity for 30 minutes. Blood was taken before and after the exercise in order to examine the levels of serum chloride. The results of this study were processed by using Wilcoxon test. Averagely, the level of serum chloride before exercise was 106,23 ± 2,208 mmol/L, and 106,90 ± 1,9 mmol/L after exercise. Statistical test result showed p = 0,007. It is concluded that there is a significant difference on the levels of serum chloride before and after light intensity exercise in the students of Medical Faculty Sam Ratulangi University. Keywords: Serum Chloride, Exercise , Light Intensity. Abstrak: Latihan fisik memicu pengeluaran keringat untuk memindahkan panas dari dalam tubuh ke lingkungan. Keringat terdiri dari air dan elektrolit, termasuk ion klorida, sehingga jika elektrolit dan air yang keluar dari tubuh tidak dikoreksi dapat menganggu homeostasis tubuh dan kesehatan. Penelitian ini bertujuan untuk mengetahui perbedaan kadar klorida serum sebelum dan sesudah latihan fisik intensitas ringan. Penelitian ini merupakan penelitian pre-eksperimental dengan pendekatan one group pretest-posttest design. Sebanyak 30 responden yang diambil menggunakan metode purposive sampling, melakukan jalan cepat diatas treadmill dengan intensitas 50-63% denyut jantung maksimum selama 30 menit. Pengambilan darah dilakukan sebelum dan sesudah latihan guna pemeriksaan kadar klorida serum. Hasil penelitian diolah menggunakan uji Wilcoxon. Hasil menunjukkan rerata kadar klorida sebelum latihan 106,23±2,208 mmol/L dan 106,90 ± 1,9 mmol/L setelah latihan. Hasil uji statistik menunjukkan p=0,007. Disimpulkan bahwa terdapat perbedaan yang signifikan kadar klorida serum sebelum dan sesudah latihan fisik intensitas ringan pada mahasiswa Fakultas Kedokteran Universitas Sam Ratulangi. Kata kunci: Klorida Serum, Latihan Fisik, Intensitas Ringan.

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Pengaruh Pemberian Konseling Gizi Terhadap Asupan Zat Gizi Dan Kadar Glukosa Darah Pada Pasien Diabetes Mellitus Tipe 2 Di Wilayah Kerja Puskesmas Motoling

Jurnal GIZIDO ◽

10.47718/gizi.v10i1.726 ◽

2018 ◽

Vol 10 (1) ◽

pp. 7-16

Author(s):

Ana B Montol ◽

Jufri Sineke ◽

Terema M.E. Kolompoy

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Carbohydrate Intake ◽

Fat Intake ◽

Nutritional Counseling ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Significant Difference ◽

Before And After

Diabetes Mellitus is a group of heterogeneous disorders characterized by elevated blood glucose levels or hyperglycemia. Counseling to people with Diabetes Mellitus about nutrition and healthy lifestyle is a method to raise awareness of Diabetes Mellitus patients to change food intake so as to improve blood glucose levels. The purpose of this study is to determine the effect of nutrition counseling on nutrient intake and blood glucose levels in patients with type 2 Diabetes Mellitus in the work area of Motoling Puskesmas. The research type is pre experiment design with one group pretest and posttest approach. The population in this study were 55 outpatient Diabetes Mellitus patients at Motoling Puskesmas, a sample of 23 outpatient Diabetes Mellitus Type 2 patients at Motoling Puskesmas. Nutrition counseling using media leaflets is given four times a month. Data Food intake was taken by using food recall 24 hours, data of glucose level of patient was taken by doing autocheck blood sugar test and for characteristic data of respondent using questioner. To determine the difference of glucose level with carbohydrate and fat intake before and after nutritional counseling was analyzed by using paired test of T test and Wilcoxon test. The results showed that there was a significant difference between carbohydrate intake before and after nutritional counseling (p = 0.002 <α 0.05). Average carbohydrate intake before counseling 359.52 g and after counseling 290.09 g a decrease of 69.43 g. For fat intake showed no significant difference in carbohydrate intake before and after nutritional counseling (p = 0.009 <α 0.05). Average fat intake before counseling 128.61 g and after counseling 75.04 g decreased 53.56 g. While blood glucose levels showed no significant difference in carbohydrate intake before and after nutritional counseling (p = 0.000 <α 0.05). Mean blood glucose levels before counseling 255 mg / dl to 202.39 mg / dl decreased 52.60 mg / dl. Conclusion, there is a significant difference between nutrient intake and blood glucose levels in patients with Diabetes Mellitus type 2 in the work area of Motoling Puskesmas.

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The Effect of Moringa Leaf Capsule on the Hemoglobin Levels in Young Women at Smp Sabbihisma Padang

KnE Life Sciences ◽

10.18502/kls.v4i15.5753 ◽

2019 ◽

Author(s):

Wiwi Sartika ◽

Herwati . ◽

Yossi Suryarinilsih

Keyword(s):

Young Women ◽

Junior High School ◽

The Body ◽

Control Group ◽

Case Group ◽

Control Groups ◽

Control Group Design ◽

Learning Abilities ◽

Significant Difference ◽

Before And After

In adolescents, the iron not only functions as energy metabolism in the body but also helps to improve learning achievement. Iron deficiency can lead to fatigue, and the concentration, memory and learning abilities can be disrupted. It can also lead to anemia, especially in young women. The purpose of this study was to determine the effect of leaf moringa capsules on the hemoglobin levels in adolescent girls in Sabbihisma Junior High School. This research used a quasi method of pretest and posttest experiment with control group design. The sample of the study were 32 people, 16 cases (moringa leaf capsule) and 16 control groups. Data collection was done through observation and laboratory examination of hemoglobin levels before and after treatment. The data were tested with paired sample t-test. The results showed that there was a significant difference in female hemoglobin levels in the case group with p = 0,000, while the control group was not significant with p = 0,091.

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The Knowledge and The Hydration Status of Junior Martial Art Athletes

COMPETITOR: Jurnal Pendidikan Kepelatihan Olahraga ◽

10.26858/cjpko.v13i2.20246 ◽

2021 ◽

Vol 13 (2) ◽

pp. 164

Author(s):

Ajeng Sindi Tirtasari ◽

Kunjung Ashadi

Keyword(s):

Female Athletes ◽

The Body ◽

Hydration Status ◽

Multiple Choice Questions ◽

Male Athletes ◽

Youth Athletes ◽

Martial Art ◽

Significant Difference ◽

Before And After ◽

The Difference

The purpose of this study was to determine the adequacy of fluids in the body during training for youth athletes of martial arts. The research method uses descriptive quantitative. Data collection techniques were obtained from urine tests, interviews, and multiple choice questions on 10 athletes consisting of five male athletes and five female athletes of martial art with a maximum age criteria of 18 years. Data analysis techniques used mean, standard deviation, percentage, graph, normality, and different test Paired Sample T Test to find out the difference in the mean. Athletes' knowledge of hydration was in the moderate category and there was a significant difference (Sig. <0.05) in the average hydration before and after training by the Madiun Regency martial art athletes.

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