scholarly journals The addition of calcium oxide with different doses in the recirculation system to improve the abalone Haliotis squamata seed production

2020 ◽  
Vol 19 (2) ◽  
pp. 199-206
Author(s):  
Eddy Supriyono ◽  
Debora Victoria Liubana ◽  
Tatag Budiardi ◽  
Irzal Effendi
Keyword(s):  
Seed Production ◽  
Calcium Oxide ◽  
Feed Efficiency ◽  
Specific Growth ◽  
Quality Parameters ◽  
Gracilaria Verrucosa ◽  
Shell Width ◽  
Recirculation System ◽  
Treatment Medium ◽  
Different Doses

Information about abalone growth is necessary to overcome the abalone culture sustainability. Water quality parameters are aspects that need to be reviewed, one of which is the calcium level in the water. The aim of this study was to determine the optimal calcium dose in recirculation system for abalone (Haliotis squamata) growth. The study was conducted with an experimental method with four treatments (calcium oxide with doses of 0, 15, 30, 45 mg/L) and three times replications. Abalone with 2.3 g body weight, 2.3 cm shell length, and 1.2 cm shell width was reared for 60 days in recirculation system and fed with Gracilaria verrucosa. The results showed that the best abalone treatment medium with the addition of calcium oxide to increase the abalone seed production was 15 mg/L CaO with 100% survival rate, 31.57±2.82% feed efficiency, and 0.56±0.06%/day specific growth rate.   Keywords: Abalone, calcium, organ composition, production, recirculation   ABSTRAK Informasi tentang pertumbuhan abalon diperlukan untuk mengatasi keberlanjutan budidaya abalon. Parameter kualitas air merupakan aspek yang perlu ditinjau. Kadar kalsium dalam air menjadi salah satu aspek yang dapat ditinjau.Tujuan dari penelitian ini adalah menentukan dosis kalsium optimal melalui sistem resirkulasi untuk pertumbuhan abalon Haliotis squamata. Penelitian ini dilakukan dengan metode eksperimental dengan empat perlakuan 3 ulangan penambahan CaO dengan dosis 0, 15, 30, dan 45 mg/L. Secara singkat, abalon dengan bobot badan 2,3 g, panjang cangkang 2,3 cm dan lebar cangkang 1,2 cm dipelihara selama 60 hari dengan sistem resirkulasi dan diberi makan dengan Gracilaria verrucosa. Hasil penelitian menunjukkan bahwa media perlakuan abalon dengan penambahan kalsium oksida terbaik untuk meningkatkan produksi abalon adalah CaO 15 mg/L dengan tingkat kelangsungan hidup 100%, efisiensi pakan 31,57 ± 2,82 %, dan laju pertumbuhan spesifik harian 0.56 ± 0.06%/hari. Kata kunci: Abalon, kalsium, komposisi organ, produksi, resirkulasi

scholarly journals Effect of Dietary Probiotics Isolated from the Gut of Tilapia, Oreochromis mossambicus (Peters, 1852) on its Growth Performance and Digestive Enzyme Activities

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Mariyam K.H ◽  
Greeshma K.P ◽  
Ramesh P.R ◽  
Pushpalatha E
Keyword(s):  
Enzyme Activities ◽  
Feed Efficiency ◽  
Specific Growth ◽  
Growth Parameters ◽  
Digestive Enzyme ◽  
Bacillus Coagulans ◽  
Oreochromis Mossambicus ◽  
Enzyme Assays ◽  
Digestive Enzyme Activities ◽  
Different Doses

A 60 days experiment was conducted to determine the effect of different doses of two probiotics on growth parameters and digestive enzyme activities of tilapia, Oreochromis mossambicus. Tilapia were fed with probiotics Lactobacillus plantarum and Bacillus coagulans isolated from the gut of tilapia, at concentrations of 1x10 2 CFU g ?1, 1 ×10 4 CFU g ?1, 1 ×10 6 CFU g ?1, 1 ×10 8 CFU g ?1 and the probiotic bacteria fed separately. The growth parameters such as %weight gain, specific growth rate (SGR), feed conv ersion ratio and feed efficiency and the digestive enzyme assays such as amylase, protease and lipase were measured. During the 60 days experiment all the parameters were measured at 15 days intervals. The results show significant improvement in digestive enzyme activities and growth parameters.

scholarly journals Cellulase hydrolyzed Indigofera zolingeriana leaf utilization as a feed ingredient for gourami fingerling

2021 ◽  
Vol 20 (2) ◽  
pp. 139-147
Author(s):  
Jefry Jefry ◽  
Mia Setiawati ◽  
Dedi Jusadi ◽  
Ichsan Achmad Fauzi
Keyword(s):  
Growth Performance ◽  
Feed Efficiency ◽  
Specific Growth ◽  
Second Step ◽  
Feed Ingredient ◽  
Protein Retention ◽  
Cellulase Enzyme ◽  
Randomized Design ◽  
Best Value ◽  
Completely Randomized Design

This study was aimed to evaluate the utilization of hydrolyzed Indigofera zolingeriana by celullase enzyme as the feed ingredient of gourami fish. This study used a completely randomized design which contained three steps, whereas each step contained four treatments and four replications. The first step performed by evaluating the Indigofera leaf meal (ILM) added with cellulase enzyme of 0 g/kg (control), 0.4 g/kg, 0.8 g/kg, and 1.2 g/kg. The second step was the digestibility test of ILM on gourami seeds. The third step was feed evaluation added with ILM as much as 0% (control), 15%, 30%, and 45% against the growth performance on gourami seeds. The gourami seeds used in the second and third steps with a weight of 13.65 ± 0.39 g/seed and 5.95 ± 0.15 g/seed, respectively. The addition of 0.8 g/kg and 1.2 g/kg cellulase enzyme could significantly decrease the crude fiber of ILM with 43.33%, besides having the best value of total, ingredient, protein, lipid, and energy digestibility. The growth performance of gourami seeds given 15% ILM added feed had the best value and insignificantly different from the control feed without ILM addition based on the specific growth rate (SGR), protein retention (PR), and feed efficiency (FE).          Keywords: Cellulase, feed, hydrolyze, Indigofera zolingeriana, Osphronemus gouramy.   ABSTRAK   Penelitian ini dilakukan untuk mengevaluasi pemanfaatan daun Indigofera zolingeriana yang dihidrolisis enzim selulase sebagai bahan baku pada pakan benih ikan gurami. Penelitian ini menggunakan rancangan acak lengkap yang terdiri atas tiga tahap, dimana masing-masing tahap terdiri dari empat perlakuan dan empat ulangan. Pada tahap pertama dilakukan evaluasi tepung daun Indigofera (TDI) yang ditambahkan enzim selulase sebesar 0 g/kg (kontrol), 0.4 g/kg, 0.8 g/kg, dan 1.2 g/kg. Pada tahap kedua dilakukan uji kecernaan bahan TDI pada benih ikan gurami. Pada tahap ketiga dilakukan evaluasi pakan yang ditambahkan TDI sebesar 0% (kontrol), 15%, 30% dan 45%  terhadap kinerja pertumbuhan pada benih ikan gurami. Benih ikan gurami yang digunakan pada penilitian tahap kedua dengan bobot 13.65 ± 0.39 g/ekor dan 5.95 ± 0.15 g/ekor. Penambahan enzim selulase pada dosis 0.8 g/kg dan 1.2 g/kg secara signifikan mampu menurunkan serat kasar TDI sebesar 43.33 % dan memberikan nilai terbaik terhadap nilai kecernaan total, kecernaan bahan, kecernaan protein, kecernaan lemak dan kecernaan energi. Kinerja pertumbuhan benih ikan gurami yang diberikan pakan yang ditambahkan TDI sebesar 15% memilki nilai terbaik dan tidak berbeda nyata terhadap pakan kontrol tanpa TDI dari aspek laju pertumbuhan spesifik (LPS), retensi protein (RP) dan efisiensi pakan (EP).   Kata kunci: Hidrolisis, Indigofera zolingeriana, Osphronemus gouramy, pakan, selulase.

scholarly journals The Affection of Fine Bubbles (FBs) Application on Growth Siamese Catfish (Pangasianodon hypophthalmus) in Aquaponic System

2020 ◽  
pp. 30-36
Author(s):  
Rahmad Afdillah ◽  
Yuli Andriani ◽  
Zahidah Hasan ◽  
. Rosidah ◽  
. Iskandar
Keyword(s):  
Water Quality ◽  
Specific Growth ◽  
Good Condition ◽  
Quality Parameters ◽  
Control Treatment ◽  
Effective Pressure ◽  
Growth And Survival ◽  
Pangasianodon Hypophthalmus ◽  
Randomized Design ◽  
Completely Randomized Design

This research aims was to determine the effective pressure on fine bubbles technology on the growth of Siamese catfish fry in the aquaponic system. This study uses a completely randomized design with four treatments and three replications. Treatment A (Control), Treatment B (FBs at a pressure of 4,5 atm), Treatment C (FBs at a pressure of 5 atm and treatment D (FBs at a pressure of 5,5 atm). The parameters observed were Specific Growth Rate, Survival Rate and water quality (Temperature, pH, DO, Ammonia) Data were analyzed using variance with the F test at a 95% confidence level. The best treatment for catfish growth was treatment D (5,5 atm pressure), which gave the highest SGR value of 7,24% and the highest SR value of 100%. The value of water quality parameters were in good condition for the growth and survival of catfish.

scholarly journals Survival and growth responses of snakehead fish Channa striata Bloch. juvenile in aerated and unaerated acid sulfate water

2017 ◽  
Vol 16 (1) ◽  
pp. 60
Author(s):  
, Purnamawati ◽  
Daniel Djokosetiyanto ◽  
Kukuh Nirmala ◽  
Enang Harris ◽  
Ridwan Affandi
Keyword(s):  
Growth Rate ◽  
Blood Glucose ◽  
Specific Growth Rate ◽  
Feed Efficiency ◽  
Specific Growth ◽  
Stocking Density ◽  
Water Volume ◽  
Growth Responses ◽  
Acid Sulfate ◽  
Channa Striata

<p><strong>ABSTRACT </strong></p><p> </p><p>The aim of the research was to analyze survival rate, specific growth rate, albumin, and feed efficiency and physiological (blood glucose, cortisol, dan haemoglobin) responses of snake head fish juvenil that reared at aerated and unaerated of tidal land water have been conducted in the laboratory. Experiments using completely randomized design with aerated and unaerated as a treatment, and each treatment has twelve replications. The snakehead fish juvenil wich length 2.4±0.2 cm and weight of 0.21±0.05 g reared in the aquarium that are size 30×25×35 cm (water volume 25 L) with a stocking density 2 juvenile/L, for 40 days. The fishes were fed with commercial feed with protein content about 40%, feeding two times a day (morning and afternoon) were at satiation. Replacement of water done every two days about 10% of the total water volume in the aquarium. The results showed that unaerated median significantly affected to biometric and physiological response of juvenile of snake head fish. The media un-aerated gives the best results shown by the higher value of survival (92%), specific growth rate (6.73%/ day), feed efficiency (78.22%), protein retention (41.91%), energy retention (30.81%) value of albumin (6.60 g/100 mL) and the haemoglobin (5.58 g/dL), and have the lowest value of cortisol (21.49 ng/L) and blood glucose (43.36 mg/100 mL).</p><p> </p><p>Keywords: acid sulfate water, growth rate, aeration, <em>Channa striata </em></p><p><em> </em></p><p> </p><p><strong>ABSTRAK </strong></p><p> </p><p>Penelitian ini bertujuan untuk menganalisis respons kelangsungan hidup, pertumbuhan spesifik, albumin, dan efisiensi pakan dan fisiologis (kortisol, glukosa darah, dan hemoglobin) juvenil ikan gabus yang dipelihara dengan dan tanpa aerasi pada media air rawa pasang surut. Penelitian menggunakan rancangan acak lengkap dengan aerasi dan tanpa aerasi sebagai perlakuan, dan masing-masing perlakuan memiliki 12 ulangan. Juvenil ikan gabus berukuran panjang 2,4±0,3 cm dan bobot 0,21±0,03 g dipelihara dalam akuarium 30×25×35 cm (volume 25 L) dengan padat tebar 2 ekor/L, selama 40 hari. Ikan diberi pakan berupa pakan komersial dengan kadar protein ±40%, pemberian pakan dua kali sehari (pagi dan sore) <em>at satiation</em>. Penggantian air dan penyiponan dilakukan dua hari sekali sebanyak 10% dari volume total dalam akuarium. Hasil penelitian menunjukkan bahwa perlakuan tanpa aerasi berpengaruh nyata terhadap respons biometrik dan fisiologis juvenil ikan gabus. Media tanpa aerasi memberikan hasil yang lebih baik ditunjukkan oleh kelangsungan hidup (92%), laju pertumbuhan spesifik (6,73%/ hari), efisiensi pakan (78,22%), retensi protein (41,91%), retensi energi (30,81%), kadar albumin (6,60 g/100 mL), dan hemoglobin (5,85 g/dL) yang lebih tinggi, sedangkan kadar kortisol (219 ng/L) dan glukosa darah (43,36 mg/100 mL) yang terendah.</p><p> </p><p>Kata kunci: media sulfat masam, pertumbuhan, aerasi, <em>Channa striata</em></p>

scholarly journals PENGARUH PEMBERIAN PROBIOTIK Bacillus spp. MELALUI PAKAN TERHADAP KELANGSUNGAN HIDUP DAN LAJU PERTUMBUHAN IKAN NILA (Oreochromis niloticus)

2016 ◽  
Vol 16 (1) ◽  
Author(s):  
Muhammad Masyarul Rusdani, Sadikin Amir Saptono Waspodo dan Zaenal Abidin
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Oreochromis Niloticus ◽  
Specific Growth ◽  
Good Combination ◽  
Randomized Design ◽  
Bacillus Spp ◽  
Completely Randomized Design ◽  
Total Bacteria ◽  
Different Doses

ABSTRAKPenelitian ini bertujuan untuk menguji kinerja bakteri probiotik Bacillus spp. dan prebiotik molase dengan dosis berbeda terhadap tingkat kelangsungan hidup dan kinerja pertumbuhan ikan nila (Oreochromis niloticus). Penelitian ini menggunakan motede eksperimen dengan rancangan acak lengkap (RAL) dan melibatkan 5 perlakuan, yaitu kontrol (K), Bacillus spp. 10 ml/kg pakan (A); Bacillus spp. 10 ml/Kg pakan + molase 10 v/v (B); Bacillus spp. 20 ml/Kg pakan (C); dan Bacillus spp. 20 ml/Kg pakan + molase 10 v/v(D). Hasil yang diperoleh menunjukkan variasi untuk total bakteri, yaitu dari 2,24x108 hingga 3,78x108 CFU/ml. Demikian juga dengan total Bacillus spp. dari 1,07x106 hingga 2,02x107 CFU/ml. Adapun untuk pengamatan tingkat kelangsungan hidup dan laju pertumbuhan tidak menunjukkan hasil yang signifikan. Meski demikian, perlakuan D memiliki hasil yang relatif lebih baik dengan nilai SR dan LPH berturut-turut 96,67% dan 1,01 %/hari. Kata kunci : probiotik Bacillus spp., prebiotik molase,  total bakteri dan Bacillus spp., tingkat kelangsungan hidup, laju pertumbuhan harian  ABSTRACTThis study was aimed to determine the good combination of probiotic bacillus spp. and prebiotic molasses with different doses to survival rate and growth performance of tilapia (Oreochromis niloticus). this study has used experimental design with completely randomized design (CRD) and five treatment, i.e. Control (K), without probiotic and prebiotic; Bacillus spp. 10 ml/kg feed (A); Bacillus spp. 10 ml/Kg feed + molasses 10 v/v (B); Bacillus spp. 20 ml/Kg feed (C); dan Bacillus spp. 20 ml/Kg feed + molasses 10 v/v (D). The results showed variation for total bacteria and total Bacillus spp. The average of total bacteria obtained in this study ranged from 2,24x108 to 3.78x108 CFU/ml. Likewise, the total Bacillus spp. were obtained from 1.07x106 to 2.02x107 CFU/ml. While for survival rate (SR) and the growth rate (DGR) did not showed significant results. However, treatment D has a relatively better then treatment else with SR and DGR respectively 96.67% and 1.01%.day-1. Keywords : probiotic Bacillus spp., prebiotic molasses, total bacteria and Bacillus spp., survival rate, specific growth rate.

scholarly journals The affection of fine bubbles (FBs) aplication on growth, feed efficiency of striped catfish, Pangasianodon hypophthalmus (Sauvage, 1878) and water quality in aquaponic system

2021 ◽  
Vol 21 (3) ◽  
pp. 277-290
Author(s):  
Yuli Andriani ◽  
Zahidah Zahidah ◽  
Rosidah Rosidah ◽  
Iskandar Iskandar
Keyword(s):  
Water Quality ◽  
Feed Efficiency ◽  
Suitable Condition ◽  
Plant Productivity ◽  
Quality Parameters ◽  
Control Treatment ◽  
Average Weight ◽  
Feed Conversion ◽  
Pangasianodon Hypophthalmus ◽  
Randomized Design

This research aims to determine the effect of fine bubble technology with different pressures on Siamese catfish seed's growth and feed efficiency in an aquaponic system. This research used a completely randomized design with four treatments and three replications. Treatment A (Control), Treatment B (FBs with a pressure of 4.5 atm), Treatment C (FBs with a pressure of 5 atm and treatment D (FBs with a pressure of 5.5 atm). The parameters observed were Specific Growth Rate, Feed Conversion Ratio, water quality (temperature, pH, DO, and ammonia), and plant productivity.  The seeds used were catfish seeds aged 16-36 days, 1-2 inches long, and weighed 0.26-0.29 grams. The container used is a fiber tub of 16 units with a size of 70 cm x 70cm x 70 cm. The feed used is commercial feed PF 1000 content crude protein 35%. The data were analyzed using variance with the F test at the 95% confidence level. The best treatment for catfish growth was treatment D (pressure 5.5 atm), which gave the highest SGR value of 7.24 ± 0.5 and FCR value of 1.19 ± 0.09. The value of water quality parameters is in suitable condition for the growth of catfish seed. The best plant productivity was found in treatment D with an average weight of 60.75 g.

scholarly journals Administration of Probiotic sanolife Mic-S on Biological Performance of Vanamei Shrimp (Litopenaeus vannamei) PL 10

2021 ◽  
Vol 3 (8) ◽  
pp. 42-57
Author(s):  
Hasim Hasim ◽  
Nadia Baidi ◽  
Syamsuddin Syamsuddin ◽  
Rully Tuiyo
Keyword(s):  
Feed Efficiency ◽  
Economic Value ◽  
Feed Conversion ◽  
Shrimp Culture ◽  
Growth And Survival ◽  
Crude Fiber Content ◽  
Weight Growth ◽  
Randomized Design ◽  
Biological Performance ◽  
Different Doses

Vannamei shrimp is a fishery commodity that has important economic value for Indonesia. This is indicated by the increasing number and value of exports of this commodity. One of the efforts to encourage the production of vaname shrimp is through the use of probiotics. sanolife-MIC is a probiotic widely used in shrimp culture. The aim of the study was to analyze the feed profile and biological performance of Vaname PL 10 shrimp fed pellets with the Sanolife MIC-S probiotic capsule at different doses. The research method used is an experimental method using a completely randomized design (CRD) with four treatments (A: probiotic 0 gr/kg feed; B: probiotic 10 gr/kg feed; C: probiotic 12 gr/kg feed; D: probiotic 14 g/kg feed) and three replicates. What was tested in the study were probiotic profile tests, growth, feed efficiency and feed conversion. The data obtained were analyzed using ANOVA. The results showed that the feed profile that was encapsulated using sanolife MIC-S, the protein value, moisture content and ash content for the feed were shown by treatment B. The best fat content was treatment C. The best crude fiber content was treatment D. Weight growth rate, ratio The best feed conversion and feed efficiency was shown by treatment B, while the length growth and survival was indicated by treatment C.

scholarly journals Production Performance of Gracilaria verrucosa using Verticulture Method with Various Wide Planting Area in Karimunjawa

Omni-Akuatika ◽  
2019 ◽  
Vol 15 (1) ◽  
pp. 47
Author(s):  
Titik Susilowati ◽  
Agus Nadlir ◽  
Alfabetian Harjuno Condro Haditomo ◽  
Seto Windarto ◽  
Dicky Harwanto ◽  
...  
Keyword(s):  
Growth Rate ◽  
Specific Growth ◽  
Mesh Size ◽  
Gracilaria Verrucosa ◽  
Production Performance ◽  
Market Demand ◽  
Seaweed Cultivation ◽  
Relative Growth ◽  
Absolute Growth ◽  
Potential Area

Gracilaria verrucosa is one type of seaweed that can be developed into a high-value product. Market demand for agar reaches 21.8% per year, but only 13.1% can be met. This is due to the low level of production of G. verrucosa in Indonesia. Utilization of G. verrucosa is still relying on aquaculture from the farm, which causes low production. Cultivation of seaweed with verticulture methods can increase the production of G. verrucosa. Karimunjawa is a potential area for seaweed cultivation. The aim of this study is to determine the effect of different density areas on the growth and production of G. Verrucosa and to determine the best density area for its production in Karimunjawa. This research used G. verrucosa wrapped in a mesh size of 0.5cm with a weight of 50g per pack. Each verticulture strap contains 4 packs and each treatment consists of 9 straps. The treatment was in the form of differences in the planting area of 25x25cm2 (A), 50x50cm2 (B) and 75x75cm2 (C). The results showed significant differences (P <0.01) between each treatment. The 75x75cm2 area has the best yield on absolute growth, relative growth rate (RGR) and specific growth rate (SGR) of 83.10 ± 4.12g; 3.96 ± 0.20% / day and 2.33 ± 0.07% / day. The best results of G. verrucosa production obtained at an area of 50x50cm2 (5.32 ± 0.26 kg / m2).

Evaluation of spring soft wheat varieties and zonal features of seed production in the Altai Territory

2021 ◽  
pp. 54-71
Author(s):  
S. V. Zharkova ◽  
E. I. Dvornikova
Keyword(s):  
Seed Production ◽  
Field Studies ◽  
Quality Parameters ◽  
Scientific Data ◽  
Soft Wheat ◽  
Forage Crops ◽  
High Quality ◽  
Wheat Varieties ◽  
Spring Soft Wheat ◽  
High Quality Seed

One of the leading grain crops in Russia is spring wheat. This is a strategic food crop of our country; it is also an important component in the structure of forage crops for monogastric animals, such as pigs and poultry. The purpose of the research was to evaluate spring soft wheat varieties of different maturity groups in order to identify genotypes as the starting material for obtaining varieties adapted to the cultivation zones, and to determine the areas optimal for the production of grain for seed purposes with high quality indicators. Field studies have been carried out in three ecologically different zones: the Priobskaya zone, the Prialtaiskaya zone, and the Prisalairskaya zone. Under the conditions of the Altai Territory, genetic sources of spring soft wheat have been identifi ed for different soil and climatic parameters of the study zones, the use of which will allow obtaining high-yielding varieties with high quality grain for specific cultivation conditions. The variability of the indicators of the characteristics of varieties in three ecologically different zones has been determined. The indicators of adaptability and stability of spring soft wheat varieties in different zones of cultivation have been determined. The optimal zones for conducting breeding work and seed production of varieties have been identifi ed. New scientific data on the quality parameters of seed grain in various agro-climatic zones of the Altai Territory have been obtained. The share of the contribution of the factors “variety”, “year”, “environment” to the variability of grain quality characteristics has been established. The economic efficiency of cultivating varieties of spring soft wheat for the production of high-quality seed grain has been determined.

scholarly journals Evaluation of Dietary Organic and Inorganic Mercury Threshold Levels on Induced Mercury Toxicity in a Marine Fish Model

Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 405 ◽  
Author(s):  
Said Majdood Raihan ◽  
Mohammad Moniruzzaman ◽  
Youngjin Park ◽  
Seunghan Lee ◽  
Sungchul C. Bai
Keyword(s):  
Marine Fish ◽  
Feed Efficiency ◽  
Specific Growth ◽  
Paralichthys Olivaceus ◽  
Inorganic Mercury ◽  
Olive Flounder ◽  
Mercury Toxicity ◽  
Potential Threat ◽  
Threshold Levels ◽  
Fish Model

Mercury as one of the most toxic elements can be present in organic or inorganic form in marine fishes, which may cause a potential threat to public health. In this study, we investigated to determine the dietary organic (O-Hg) and inorganic (I-Hg) mercury threshold levels on induced mercury toxicity in juvenile olive flounder, Paralichthys olivaceus as a marine fish model. Twenty-eight fish averaging 3.1 ± 0.05 g (mean ± SD) were arbitrarily assigned to each of 27 tanks. Each tank was arbitrarily restricted to triplicates of nine experimental diets for eight weeks. The experimental diets were manufactured to contain 0 (Control), 10 (I-Hg10, O-Hg10), 20 (I-Hg20, O-Hg20), 40 (I-Hg40, O-Hg40) and 160 (I-Hg160, O-Hg160) mg/kg diet in organic form as methylmercury (MeHg) or in inorganic form as mercuric chloride (HgCl2). At the termination of the experimental trial, weight gains (WGs) of fish fed the control and 10 (I-Hg10, O-Hg10) diets were remarkably higher than those of fish fed the 20 (I-Hg20, O-Hg20), 40 (I-Hg40, O-Hg40) and 160 (I-Hg160, O-Hg160) (p < 0.05). Specific growth rate and feed efficiency of fish fed control and 10 (I-Hg10, O-Hg10) diets were significantly higher than those of fish fed 40 (I-Hg40, O-Hg40) and 160 (I-Hg160, O-Hg160) diets. In comparison to the dietary inorganic mercury, dietary MeHg bioaccumulation rates were significantly higher in the tissue levels according to the dietary inclusion levels. MeHg accumulated mostly in kidney, followed by liver and gill tissues. HgCl2 accumulated in tissues, in decreasing order, liver > kidney > gills. A broken-line regression model for percentage of WG indicated that the threshold toxicity level for an Hg-incorporated diet of juvenile olive flounder could be 13.5 mg Hg/kg in the form of HgCl2 and 8.7 mg Hg/kg in the form of MeHg.

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