scholarly journals Pengendalian Penyakit Pustul Xanthomonas axonopodis pv. glycines Pada Kedelai Dengan Bacillus spp. Asal Filosfer Gulma di Pertanaman Kedelai

2020 ◽  
Vol 2 (2) ◽  
pp. 53
Author(s):  
Siti Rahayu ◽  
Suhartiningsih Dwi Nurcahyanti
Keyword(s):  
Incubation Period ◽  
Infection Rate ◽  
Soybean Plant ◽  
Xanthomonas Axonopodis ◽  
Severity Of Disease ◽  
Bacillus Spp ◽  
Number Of Leaves ◽  
Treatment Application ◽  
Number Of Branches

Soybean pustule disease by Xanthomonas axonopodis pv. glycines (Xag) is one of the important diseases of soybean plant. Application Bacillus spp. origin of weed phyllosphere has a great potential as an alternative to control because it is isolated to origin of same region as Xag is the phyllosphere. Research by Nurcahyanti and Ayu obtained the best five isolates of Bacillus spp. of weeds phyllosphere in soybean cropping in inhibiting Xag in vitro. This study used the complete random draft (RAL) 6 treatment, 4 repeats, each unit consists of 4 plants wich treatment application of 5 isolates of Bacillus spp. namely K = control; A = Bacillus JG 1.3; B = Bacillus JG 3.6; C = Bacillus JG 1.4.1; D = Bacillus BGd 1.1; E = Bacillus Bp 2.2. The results showed that application of Bacillus spp. could inhibit Xag in vitro with bacteriostatic mechanism and isolates Bacillus BGd 1.1 has the greatest inhibition of 14.25 mm. Fifth Bacillus spp. can suppress the development of disease and isolates Bacillus BGd 1.1 has best results with the incubation period during 13 HSI, the severity of disease 10.07%, infection rate 0.045 unit/day as well as the effectiveness of control 85.72%. The fifth isolates of Bacillus spp. can not increase the number of leaves but can increase number of branches and isolates Bacillus JG 1.3, Bacillus JG 1.4.1 and Bacillus BGd 1.1 can increase the height of soybean crop.

scholarly journals POTENSI AGENSIA HAYATI DAN NABATI DALAM MENGENDALIKAN PENYAKIT BUSUK RIMPANG JAHE

2005 ◽  
Vol 5 (1) ◽  
pp. 50-57
Author(s):  
Loekas Soesanto ◽  
Sudarmono Sudarmono ◽  
Nur Prihatiningsih ◽  
Abdul Manan ◽  
Endang Iriani ◽  
...  
Keyword(s):  
Incubation Period ◽  
Infection Rate ◽  
Trichoderma Harzianum ◽  
Block Design ◽  
Central Java ◽  
Randomized Block Design ◽  
Ginger Rhizome ◽  
Number Of Leaves ◽  
Central Java Province ◽  
Rhizome Rot

Potency of biological and botanical agents in controlling ginger rhizome rot. Research aimed at knowing integrity of various control agents in controlling causal agent of ginger rhizome rot. The research was carried out at Gowak Village, Pringsurat District, Temanggung Regency, Central Java Province, from November up to May 2004. Randomized Block Design was used with treatments of biological (Bacillus sp. with density of x  1010 cfu ml-1 suspension and Trichoderma harzianum 20 g crop-1), of botanical (clove leaves powder of 20 g crop-1 and neem seeds extract of 25 ml crop-1), and synthetical agents (benomil with concentration of 2 g l-1) either alone or in combination, with three replicates. Variables observed were incubation period, disease intensity, infection rate, number of leaves per crop, crop height, and ginger production per hectare. Result of the research showed that the location was endemic of the disease. The treatments could generally retard the incubation period and the infection rate, decrease the disease intensity, and increase the ginger production. The highest ginger yield was obtained from the plot given Trichoderma harzianum (Trikonis), either alone or in combination with the dry clove leaves of 10 g crop-1 as many as four times with interval of seven days started from seedling planted.

scholarly journals PENGARUH KOMPOSISI BIOFERTILIZER CAIR TERHADAP PERTUMBUHAN TANAMAN KEDELAI DI LAHAN GAMBUT (The Effect of Liquid Biofertilizer Composition on Soybean Plant Growth in Peat Land)

AgriPeat ◽  
2019 ◽  
Vol 19 (01) ◽  
pp. 30-36
Author(s):  
Administrator Journal
Keyword(s):  
Plant Height ◽  
Peat Soil ◽  
Marginal Land ◽  
Soybean Plant ◽  
Randomized Design ◽  
Soil Media ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Soybean Plants ◽  
Number Of Branches

ABSTRACThis study aims to find the most potent combination of local microorganisms as a liquidbiofertilizer on marginal land, such as peatland. The study used Completely Randomized Design onpeat soil media in polybags, with 8 (eight) treatments and 4 (four) replicates, namely liquidbiofertilizer composition comprising a group of local microorganisms: 1) KHY, 2) IBT, 3) KHY +IBT, 4) KHY + IGT, 5) IBT + IGT, 6) KHY + IBT + IGT, 7) EM4 (Control +), 8) Aquadest(Controls -). The variables observed were plant height, number of leaves, number of branches,number of flowers of soybean crop. The results showed that the composition of the liquidbiofertilizer EM4 gave the best results on the parameters of plant height, the number of leaves, thenumber of branches, and the amount of flowers on soybean plants tested in peat soil media. Thecombined composition of local microorganisms KHY + IBT + IGT containing bacterial populationsof phosphate solvent, nitrogen-fixing bacteria, and cellulitic fungi, are still in the same group asEM4 in the treatment of the number of leaves and the number of plant flowers, therefore thecomposition of these potential local microorganisms is developed to become a liquid biofertilizerKeywords: Liquid Biofertilizer, Soybean Growth, Peat Land

scholarly journals Chemical Mutagens Affecting in vitro Behavior of Gardenia jasminoides

2020 ◽  
Vol 30 (2) ◽  
pp. 209-218
Author(s):  
Eman Tawfik ◽  
Mohamed Fathy
Keyword(s):  
Sodium Azide ◽  
Morphological Traits ◽  
Lateral Roots ◽  
Guanidine Hydrochloride ◽  
Leaf Length ◽  
Chemical Mutagens ◽  
Gardenia Jasminoides ◽  
Number Of Leaves ◽  
Number Of Branches

The chemical mutagens, namely sodium azide and guanidine hydrochloride (30, 40 and 50 mM) for each were applied for the in vitro culture of Gardenia jasminoides Ellis. Eight morphological traits were evaluated to calculate the result of different concentrations of mutagens compared to control. In sodium azide, all morphological traits decreased compared to control, except the number of branches and leaves and leaf length, which increases compared to control. Unlike guanidine hydrochloride, all the morphological traits are enhanced in mutants rather than control. The polymorphism percentage resulted from genetic variation varied (57.71% in the case of sodium azide and 67.59% in guanidine hydrochloride). Hence, guanidine hydrochloride is a more powerful mutagen and causes more genetic variations and instability than sodium azide, as was reflected in morphological parameters (i.e., fresh weight, shoot length, root length, number of lateral roots, number of leaves, numbers of branches, leaf length, and leaf width) and physiological traits (i.e., chlorophyll pigmentation and carotenoids). Plant Tissue Cult. & Biotech. 30(2): 209-218, 2020 (December)

scholarly journals تأثير تراكيز مختلفة من السكر في بيئة زراعة الأنسجه على نمو نبات الورد صنف ساره (Sara)

2020 ◽  
Vol 16 (1) ◽  
pp. 49-62
Author(s):  
محي الدين جمعة عبد الله
Keyword(s):  
Growth Parameters ◽  
Growth Media ◽  
Fresh Weight ◽  
Negative Results ◽  
Randomized Design ◽  
Significant Difference ◽  
Number Of Leaves ◽  
Completely Randomized Design ◽  
Number Of Branches

The effect of adding different sugar concentrations of five treatments (T1, T2, T3, T4, T5) on rose growth CV Sarah in vitro was carried out in the Sudan University Laboratories. Data were collected for plant height, number of branches, number of leaves, and fresh weight. Statistical analysis was conducted using a completely randomized design, to find the least significant difference to compare between treatment means for MS (Murashige and Skoog) medium. Data analysis showed that the application of sugar at concentrations of 15, 30, and 45gms gave the highest means for the different growth parameters and fresh weight at a significant level, whereas the addition of sugar at 60gms concentration and sugar-free gave no significant increases. This showed clearly that addition of sugar was essential for plant growth, greater concentrations gaviz 60gm negative results, Addition of sugar at the above-mentioned concentration from 15mg,45gm to parameters studied the growth media in vitro gave a positive increase in rose growth.

Epidemiological factors influencing the success of roguing for the control of bunchy top disease of bananas in New South Wales

1978 ◽  
Vol 29 (3) ◽  
pp. 535 ◽  
Author(s):  
RN Allen
Keyword(s):  
Incubation Period ◽  
Infection Rate ◽  
Detection Efficiency ◽  
Late Summer ◽  
Disease Symptoms ◽  
Factors Influencing ◽  
South Wales ◽  
Number Of Leaves ◽  
Time Of Year ◽  
Banana Leaves

An infection chain was reconstructed from historical data of a typical outbreak of bunchy top disease of bananas to determine factors influencing the seasonal occurrence and eradication of disease under roguing. The results were described by a mathematical model involving four random variables (incubation period, relative infection rate, detection efficiency and eradication efficiency) and one fixed variable (inspection interval). The model successfully predicted the course of roguing in an independent disease outbreak. The incubation period of the disease depended on the time taken for two banana leaves to emerge after inoculation. At Alstonville, N.S.W., the incubation period varied from 19 days in summer to 125 days in winter. The relative infection rate varied on average from 0.027 to 0.001 new infections per old infection per day in summer and winter respectively. Efficiency of disease detection by experienced inspectors depended on the number of leaves displaying disease symptoms and, equally, on time of year. Under the normal 3-weekly inspection routine, detection efficiency varied from 0.94 in summer to 0.19 in winter, plants being found on average at the two-leaf stage of disease. The efficiency with which banana growers eradicated diseased plants following their detection was found to be a characteristic of the grower concerned but was, in general, poorest in late summer.

scholarly journals The effect of organic fertilizers on growth several varieties of soybeans

2021 ◽  
Vol 883 (1) ◽  
pp. 012051
Author(s):  
M Rizwan ◽  
M Dalimunthe ◽  
I A Pasaribu ◽  
H Satriawan
Keyword(s):  
Leaf Area ◽  
Plant Height ◽  
Block Design ◽  
Organic Fertilizer ◽  
Organic Fertilizers ◽  
Chicken Manure ◽  
Soybean Plant ◽  
Randomized Block Design ◽  
Number Of Leaves ◽  
Number Of Branches

Abstract This study aims to analyse the effect of three types of organic fertilizers on the vegetative growth several varieties of soybeans. This study used a factorial randomized block design with two treatment factors, namely organic fertilizers and varieties. The first factor is: organic fertilizer from 3 levels, namely: K1 (1.5 kg of compost), K2 (1.5 kg of goat manure), and K3 (1.5 kg of chicken manure). The second factor is: soybean plant varieties consist of 4 levels, namely: V1 (Dena 1), V2 (Devon 1), V3 (Anjasmoro) and V4 (Derap 1). The parameters observed were plant height, number of branches, number of leaves, amount of chlorophyll, leaf area, and flowering age. The results of our research is organic fertilizers have a significant effect and are able to increase the growth of soybean plants except for leaf area and flowering age. Types of varieties had a significant effect and were able to increase plant height, number of branches, leaf area, and flowering age, but had no significant effect on the parameters of the number of leaves and the amount of chlorophyll. Moreover, there isn't interaction effect between the two treatments for all parameters.

scholarly journals In vitro morphogenetic response of apple (Malus domestica Borkh.) and pear (Pyrus communis L.) to the elevated levels of copper and myo-inositol

2012 ◽  
Vol 65 (3) ◽  
pp. 43-48 ◽  
Author(s):  
Rafail S. Toma ◽  
Gharbia N.Y. Danial ◽  
Atheel H. Habash
Keyword(s):  
Root Formation ◽  
Control Treatment ◽  
Medium Components ◽  
Pyrus Communis L ◽  
Number Of Leaves ◽  
Morphogenetic Potential ◽  
The Mean ◽  
Culture Growth ◽  
Number Of Branches

The elevated levels of copper and <em>myo</em>-inositol in the MS medium were demonstrated to enhance culture growth and morphogenetic response of apple and pear explants. The results revealed that the highest number of branches per explant (2.80) for apple was obtained from the levels of 0.0+ 100 and 0.050+400 mg/l of both copper and <em>myo</em>-inositol, respectively (C1M2 and C4M4), while for pear 3.40 branches per explant were achieved from the same treatment. The mean length of branches was significantly lower in the case of the control treatment (the absence of copper and inositol). The highest number of leaves per explant (29.73 and 29.80) for both apple and pear, respectively, was recorded for treatment C4M4 (0.050+ 400 mg/l of both copper and <em>myo</em>-inositol, respectively. At the rooting stage, the elevated levels of copper and <em>myo</em>-inositol were very effective in stimulating root formation in both apple and pear shoots. The highest number of roots in apple (2.00 roots/ explant) was achieved while using 0.100+ 800 (C5M5) of both copper and <em>myo</em>-inositol, whereas the highest number of roots for pear (3.17 roots/ explant) was recorded for C6M6 (0.200+ 1600). The highest mean length of roots for apple reached 1.23 cm in treatment C3M3 and 1.10 cm for pear in treatment C6M6. These data suggest that the higher levels of copper and <em>myo</em>-inositol enabled shoot and root formation in the explants, and it might be necessary to use higher levels of these two medium components in order to enhance morphogenetic potential of explants.

scholarly journals Diversity of Bacillus spp. from soybean phyllosphere as potential antagonist agents for Xanthomonas axonopodis pv. glycines causal of pustule disease

2021 ◽  
Vol 22 (11) ◽  
Author(s):  
Suhartiningsih Dwi Nurcahyanti ◽  
Wiwiek Sri Wahyuni ◽  
Rachmi Masnilah ◽  
Anggi Anwar Hendra Nurdika
Keyword(s):  
Biological Agents ◽  
Morphological Observation ◽  
Rrna Gene ◽  
Xanthomonas Axonopodis ◽  
Bacillus Spp ◽  
Biochemical Testing ◽  
The 16S Rrna Gene ◽  
Potential Antagonist ◽  
Important Disease

Abstract. Nurcahyanti SD, Wahyuni WS, Masnilah R, Nurdika AAH. 2021. Diversity of Bacillus spp. from soybean phyllosphere as potential antagonist agents for Xanthomonas axonopodis pv. glycines causal of pustule disease. Biodiversitas 22: 5003-5011. Pustule disease caused by Xanthomonas axonopodis pv. glycines (Xag) is an important disease in soybean. Bacteria from soybean phyllosphere is one of the potential biological agents against this disease. This is because the microorganisms have similarity. This study aimed to determine the diversity and species of bacteria from the soybean phyllosphere that have potential as biological agents. The research was carried out by morphological observation, physiological - biochemical testing,  and molecularly with BOX AIR primer. Molecular identification was carried out by amplifying the 16S-rRNA gene with 27F and 1492R primers. The results showed that 11 isolates could inhibit Xag in vitro which showed morphological, biochemical, and molecular diversity. These bacteria were identified as Bacillus spp. which consisted of 4 groups, namely Bacillus siamensis, B. subtilis. B. amyloliquifaction and B. velezensis. The diversity of phyllosphere bacteria allows them to be used as biological agents because they do not inhibit each other and have diverse living abilities in various environmental conditions.

scholarly journals PENGENDALIAN PENYAKIT LAYU BAKTERI (RALSTONIA SOLANACEARUM) PADA TANAMAN TOMAT DENGAN PENYAMBUNGAN BATANG BAWAH TAHAN

2019 ◽  
Vol 2 (1) ◽  
pp. 295-306
Author(s):  
Annisatul Choiriyah ◽  
Suhartiningsih Dwi Nurcahyanti
Keyword(s):  
Incubation Period ◽  
Infection Rate ◽  
Bacterial Wilt ◽  
Wilt Disease ◽  
Natural Resistance ◽  
Observation Data ◽  
Tomato Plants ◽  
Bacterial Wilt Disease ◽  
Severity Of Disease ◽  
Randomized Design

Tomato plant is one of horticultural commodities that plays an important role in agricultural of Indonesia. The production of tomato is constrained by bacterial wilt (R. solanacearum). The development of the disease will increase rapidly in rainy season. This causes the production of tomato decreases for about 30-60%. The alternative effort to control the bacterial wilt (R. solanacearum) done by grafting the commercial tomato plants that are susceptible to noncommercial tomato that have natural resistance to the infection of this pathogen. Suseptible tomato Betavila F1 variety is used for scion. As resistant rootstocks are Rewako F1 and Mawar variety. The grafting done through splice grafting. The experimental design used was Complete Randomized Design with 5 treatments of 4 repetitions with each unit consisted of 5 plants. The observed parameters were incubation period, incidence of disease, severity of disease, infection rate and plant growt. The results of observation data were analyzed by using variance. The grafting treatment between Rewako F1 + Betavia F1 varieties became the best result to inhibit the bacterial wilt of R. solanacearum incubation period 14 Day After Inculation (DAI), incidence of disease 28,00%, infection rate 0,00500 unit/day and necrosis in stem 5,50%. The grafting treatment was not able to increase the component of plant growth. Keywords: Grafting, Tomato, Bacterial wilt disease

Effect of Temperature on ADP-Induced Platelet Aggregation

1973 ◽  
Vol 29 (01) ◽  
pp. 183-189
Author(s):  
C. A Praga ◽  
E. M Pogliani
Keyword(s):  
Platelet Aggregation ◽  
Incubation Period ◽  
Room Temperature ◽  
Important Variable ◽  
Practical Significance ◽  
Effect Of Temperature ◽  
Induce Platelet Aggregation ◽  
Cuvette Holder ◽  
Exact Temperature

SummaryTemperature represents a very important variable in ADP-induced platelet aggregation.When low doses of ADP ( < 1 (μM) are used to induce platelet aggregation, the length of the incubation period of PRP in the cuvette holder of the aggregometer, thermostatted at 37° C, is very critical. Samples of the same PRP previously kept at room temperature, were incubated for increasing periods of time in the cuvette of the aggregometer before adding ADP, and a significant decrease of aggregation, proportional to the length of incubation, was observed. Stirring of the PRP during the incubation period made these changes more evident.To measure the exact temperature of the PRP during incubation in the aggre- gometer, a thermocouple device was used. While the temperature of the cuvette holder was stable at 37° C, the PRP temperature itself increased exponentially, taking about ten minutes from the beginning of the incubation to reach the value of 37° C. The above results have a practical significance in the reproducibility of the platelet aggregation test in vitro and acquire particular value when the effect of inhibitors of ADP induced platelet aggregation is studied.Experiments carried out with three anti-aggregating agents (acetyl salicyclic acid, dipyridamole and metergoline) have shown that the incubation conditions which influence both the effect of the drugs on platelets and the ADP breakdown in plasma must be strictly controlled.

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