Chemical Mutagens Affecting in vitro Behavior of Gardenia jasminoides

The chemical mutagens, namely sodium azide and guanidine hydrochloride (30, 40 and 50 mM) for each were applied for the in vitro culture of Gardenia jasminoides Ellis. Eight morphological traits were evaluated to calculate the result of different concentrations of mutagens compared to control. In sodium azide, all morphological traits decreased compared to control, except the number of branches and leaves and leaf length, which increases compared to control. Unlike guanidine hydrochloride, all the morphological traits are enhanced in mutants rather than control. The polymorphism percentage resulted from genetic variation varied (57.71% in the case of sodium azide and 67.59% in guanidine hydrochloride). Hence, guanidine hydrochloride is a more powerful mutagen and causes more genetic variations and instability than sodium azide, as was reflected in morphological parameters (i.e., fresh weight, shoot length, root length, number of lateral roots, number of leaves, numbers of branches, leaf length, and leaf width) and physiological traits (i.e., chlorophyll pigmentation and carotenoids). Plant Tissue Cult. & Biotech. 30(2): 209-218, 2020 (December)

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Differentiation of morphological traits of common tansy (Tanacetum vulgare L.) originating from different stands

Acta Agrobotanica ◽

10.5586/aa.2008.047 ◽

2012 ◽

Vol 61 (2) ◽

pp. 189-194

Author(s):

Tadeusz Kęsik ◽

Anna E. Wojciechowska ◽

Karolina Pitura

Keyword(s):

Chemical Composition ◽

Soil Ph ◽

Morphological Traits ◽

Leaf Length ◽

Stem Length ◽

Growth Trend ◽

Tanacetum Vulgare ◽

Total Leaf ◽

Number Of Leaves ◽

Number Of Branches

The aim of the study was to determine the effect of pH and soil chemical composition on morphological traits of common tansy (<i>Tanacetum vulgare</i> L.). A two-year study was conducted based on observations, measurements and analyses of material collected from 24 stands in the following regions: Równina Włocławska (Włocławek Plain), Wyżyna Lubelska (Lublin Upland), Padół Zamojski (Zamość Depression), Działy Grabowieckie (Grabowiec Divide) Płaskowyż Suchedniowski (Suchedniów Plateau), Wysoczyzna Siedlecka (Siedlce High Plain) and Niecka Połaniecka (Połaniec Basin). Morphological traits of tansy shoots were determined: number of plants per clump, stem length, number of leaves per plant, total leaf length, petiole length, number of branches from the main stem, number of flower heads per corymb and flower head diameter. A soil analysis was performed determining the content of magnesium, potassium and phosphorus available for plants. The soil pH was also determined. The collected results indicate significant differentiation of the investigated samples, both in terms of morphological traits of the shoots and the chemical composition of the soils. A negative correlation was found between soil pH and number of leaves per shoot as well as number of branches per corymb. A positive correlation was noted between soil pH and total leaf length as well as between magnesium content and number of flower heads per corymb. The increased content of available phosphorus in the soil was associated with petiole shortening. A growth trend in the tansy shoot length was observed at larger amounts of available magnesium in the soil. On soils with low nutrient availability and a low pH, a reduced population density of the species in question was observed.

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GROWTH OF ‘PRATA-ANA’ BANANA’S MICROSHOOTS CLONE GORUTUBA FROM SYNTHETIC SEEDS:SUBSTRATES AND BAP CONCENTRATION

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452017892 ◽

2017 ◽

Vol 39 (5) ◽

Cited By ~ 3

Author(s):

JÚLIO CÉSAR GOMES PEREIRA ◽

SELMA SILVA ROCHA ◽

LUCIANA CARDOSO NOGUEIRA LONDE ◽

MARCELA CAROLINE BATISTA DA MOTA ◽

PABLO FERNANDO SANTOS ALVES ◽

...

Keyword(s):

Culture Medium ◽

Economic Importance ◽

Leaf Length ◽

Naphthalene Acetic Acid ◽

Synthetic Seed ◽

Ms Medium ◽

World Production ◽

Number Of Leaves ◽

Alginate Matrix

ABSTRACT The banana crop stands out as an activity of great social and economic importance in Brazil, which occupies the fifth place in world production. Synthetic seed production is becoming promising for a micropropagation and in vitro conservation. The aim of the study was to analyze the conversion and growth of ‘Prata-anã’ banana’s microshoots clone Gorutuba from synthetic seed in MS medium and vermiculite, different substrates and concentrations of BAP (6-benzylaminopurine) associated with ANA (acetic naphthalene acid) in the constitution of its capsule were tested. The microshoots were immersed in the sodium alginate matrix (3%) and dripped in a solution of CaCl2.2H2O (100 mM) for complexation and then in KNO3 solution (100 mM) to decomplex. The experimental design was completely randomized in a 2 x 5 factorial design (substrate x BAP concentrations), containing different substrates (MS culture medium and vermiculite) and BAP concentrations (2.22, 4.44, 6.66, 8.88 and 13.32 µmol L-1) associated with NAA (naphthalene acetic acid) 0.54 µmol L-1, totaling 10 treatments, with 4 replicates, and that each replicate containing 5 seeds. The evaluations of conversion, number of leaves, leaf length, leaf height, number of roots, root length and oxidation were performed at 30 and 60 days.The use of the MS medium provided better growth results in relation to vermiculite as substrate, in which the different BAP concentrations did not differ from each other. It was found that, in MS culture medium, BAP concentrations above 8.88 µmol L-1 in the capsule composition are not indicated for microshoots growth.

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In vitro development of Encyclea cordigera in different concentrations of honey

Ciência Rural ◽

10.1590/0103-8478cr20150046 ◽

2015 ◽

Vol 46 (4) ◽

pp. 590-592 ◽

Cited By ~ 2

Author(s):

Cibele Mantovani ◽

Kathia Fernandes Lopes Pivetta

Keyword(s):

Culture Media ◽

Leaf Length ◽

In Vitro Development ◽

Randomized Design ◽

Sucrose Medium ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Set Up ◽

Vitro Development

ABSTRACT: The objective of this paper was to evaluate the effects of different honey concentrations in culture media, in comparison to sucrose medium, for the in vitro development of the epiphytic Encyclea cordigera orchid, in order to improve the process of propagation of the species. The in vitro germination was prepared on a reduced Murashige & Skoog (MS) medium. After 90 days, the seedlings were divided into different treatments, where they remained for another 90 days. Six treatments were set up (30g L-1 of sucrose; 15, 30, 45, and 60g L-1 of honey; and absence of any carbohydrates) in a completely randomized design. Plants were removed from the vials 270 days after the start of the experiment, and the number of roots, length of the largest leaf, length of the longest root, number of leaves, and fresh and dry masses were evaluated. Data concerning the number of leaves and roots were (x+1)1/2 transformed and subjected to an analysis of variance (ANOVA); the means were compared by a Tukey's test set at 5% probability. Medium containing 60g L-1 of honey proved to be superior to the sucrose medium traditionally used, favoring the in vitro growth and development of Encyclea cordigera. This medium can therefore be recommended for the propagation of this species, which is usually cultivated as an ornamental plant.

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Pengaruh Dosis Pupuk KCl dan Persentase Defoliasi terhadap Pertumbuhan dan Hasil Tanaman Tin (Ficus carica L.)

Jurnal Ilmiah Mahasiswa Pertanian ◽

10.17969/jimfp.v5i2.14928 ◽

2020 ◽

Vol 5 (2) ◽

pp. 81-90

Author(s):

Raudatul Jannah ◽

Nurhayati Nurhayati ◽

Cut Nur Ichsan

Keyword(s):

Significant Interaction ◽

Leaf Length ◽

Ficus Carica ◽

Growth And Yield ◽

Leaf Width ◽

Harvest Time ◽

Branch Diameter ◽

Number Of Leaves ◽

Banda Aceh ◽

Number Of Branches

Abstrak. Penelitian ini bertujuan untuk mengetahui pengaruh dosis pupuk KCl dan persentase defoliasi yang tepat serta interaksi antara kedua faktor tersebut terhadap pertumbuhan dan hasil tanaman tin. Penelitian ini dilaksanakan di Rumah Kasa komplek Fakultas Pertanian, Universitas Syiah Kuala, Darussalam, Banda Aceh. Pelaksanaan penelitian dimulai dari bulan Juli sampai September 2019. Rancangan penelitian yang digunakan adalah Rancangan Petak Terpisah pola RAK faktorial 3 x 2 dengan 3 ulangan. Faktor yang diteliti yaitu pengaruh dosis pupuk KCl yang terdiri atas 3 taraf (0 g-1tan-1thn, 700 g-1tan-1thn, 800 g-1tan-1thn) dan persentase defoliasi yang terdiri atas 2 taraf (30% dan 60%). Hasil penelitian menunjukkan bahwa dosis pupuk KCl berpengaruh sangat nyata terhadap pertambahan jumlah daun 2, 4, 6, 8 dan 10 MSP (minggu setelah perlakuan) , pertambahan panjang daun 2 dan 10 MSP, pertambahan lebar daun 2 dan 10 MSP, pertambahan jumlah cabang 2 dan 10 MSP, pertambahan diameter cabang 2 dan 10 MSP, pertambahan jumlah buah 2, 4, 6, 8 dan 10 MSP dan jumlah buah total serta berat buah 2, 4, 6, 8 dan 10 MSP dan berat buah total. Pertumbuhan dan hasil tanaman tin yang terbaik dijumpai pada dosis pupuk KCl 800 g-1tan-1thn dengan pertambahan hasil rata-rata 15,4 t. ha-1untuk2 bulan masa panen. Hasil penelitian menunjukkan bahwa persentase defoliasi berpengaruh sangat nyata terhadap pertambahan jumlah daun 2, 4, 6, 8 dan 10 MSP, pertambahan panjang daun 2 dan 10 MSP, pertambahan lebar daun 2 dan 10 MSP, pertambahan jumlah cabang 2 dan 10 MSP, pertambahan diameter cabang 2 dan 10 MSP, pertambahan jumlah buah 2 dan 4 MSP dan berat buah 10 MSP total, berpengaruh nyata terhadap jumlah buah 6, 8 dan 10 MSP dan jumlah buah total serta berat buah 2, 4, 6, 8 MSP. Pertumbuhan dan hasil tanaman tin terbaik dijumpai pada defoliasi daun 60% dengan pertambahan hasil rata-rata 12,6 t. ha-1untuk2 bulan masa panen. Terdapat interaksi yang sangat nyata antara dosis pupuk KCl dan persentase defoliasi terhadap pertambahan jumlah daun 4, 6 dan 8 MSP, pertambahan panjang daun 2 dan 10 MSP, pertambahan lebar daun 2, jumlah cabang 2 dan 10 MSP, pertambahan diameter cabang 2 dan 10 MSP dan jumlah buah 2 MSP dan berpengaruh nyata terhadap pertambahan jumlah daun 2 MSP. Pertumbuhan dan hasil tanaman tin terbaik dijumpai pada perlakuan dosis pupuk KCl 800 g-1tan-1thn dengan defoliasi 60%.The Effect of Potassium Chloride (KCl) Fertilizer Doses and DefoliationPercentage on the Growth and the Yield of Fig (Ficus carica L.)Abstract. This research aims to examine the effect of potassium chloride (KCl) fertilizer doses and defoliation percentage on the growth and the production of Fig (Ficus carica L.) and the interaction between these factors on the growth and production of fig plants. This research was conducted at the Screen House of Faculty of Agriculture, Syiah Kuala University, Darussalam, Banda Aceh. The research was carried out from July to September 2019. The research used split-plot design of 3 x 2 factorial RCBD with 3 replications. KCl fertilizer doses consisting of 3 levels (0 g tree-1 yr-1,700 g tree-1yr-1, 800 g tree-1 yr-1) and defoliation percentage consisting of 2 levels (30 % and 60 %). The result shows that KCl fertilizer dose has a very significant effect on increasing the number of leaves to 2, 4, 6, 8 and 10 WAT (week after treatment), increasing leaf length at 2 and 10 WAT, leaf width at 2 and 10 WAT, the number of branches at 2 and 10 MSP, branch diameter at 2 and 10 WAT, the number of fruits at 2, 4, 6, 8 and 10 WAT, and fruit weight at 2, 4, 6, 8 and 10 WAT. The best growth and yield of fig plants were found at a dose of KCl 800 g tree-1 yr-1with 15,4 t. ha-1 increasing fot two mounths harvest time. Percentage of defoliation has a very significant effect on increasing the number of leaves at 2, 4, 6, 8 and 10 WAT, the number of branches at 2 and 10 MSP, the number of fruits at 6 WAT, significantly affected in increasing the number of leaves at 2 and 10 WAT, leaf width at 2 WAT, the number of branches at 2 MSP, branch diameter at 2 and 10 WAT, the number of fruits at 4, 8 and 10 WAT. The best growth and yield of fig plants were found at 60% defoliation treatment1with 12,6 t. ha-1 increasing fot two mounths harvest time. Thus, there was a very significant interaction between the dose of KCl fertilizer and the percentage of defoliation on the increasing the number of leaves at 4, 6 and 8 WAT), leaf length at 2 and 10 WAT, leaf width at 2 WAT, the number of branches at 2 and 10 MSP, branch diameter at 2 and 10 WAT, the number of fruits at 2 WAT and significant interaction on the number of leaves to 2 WAT. The best growth and yield of fig plants were found in a combination of a dose of 800 g tree-1 yr-1 of KCl fertilizer with 60% of defoliation.

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The Growth Improvement of Grammatophyllum scriptum (Lindl.) Bl. In Vitro Plantlet using Photoautotrophic Micropropagation System

Indonesian Journal of Biotechnology ◽

10.22146/ijbiotech.27167 ◽

2017 ◽

Vol 21 (2) ◽

pp. 109 ◽

Cited By ~ 1

Author(s):

Aries Bagus Sasongko ◽

Asruwaidah Fatumi ◽

Ari Indrianto

Keyword(s):

Carbon Dioxide ◽

Root Length ◽

Unit Area ◽

Leaf Length ◽

Growth Increase ◽

Photoautotrophic Micropropagation ◽

Average Quantity ◽

Chlorophyll A And B ◽

Number Of Leaves

To improve the growth of Grammatophyllum scriptum (Lindl.) Bl. in vitro plantlet, a photoautotrophic micropropagation system (PMS) was developed by growing in vitro plantlet on VW medium with varying concentration of sucrose (0, 5, 10, and 20 g/L) and additional carbon dioxide from the air (bottle covered with cap or filter). The result showed that the leaf length would increase up to 6.5 cm with PMS and it would keep growing by the adding of 5 g/L sucrose. Average number of leaves increased by 6.7 strands with PMS and the addition of sucrose increased the average quantity of leaves up to 7.7 strands. Average number and root length would increase with PMS and would even increase more with 5 g/L sucrose addition. PMS with 5 g/L sucrose can increase chlorophyll a and b concentration. The number of stomata per unit area in PMS was lower than closed culture. This shows that PMS can increase the growth of G. scriptum in vitro plantlet and the growth increase would be effective if it is combined with sucrose addition.

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Iron concentrations in the in vitro cultivation of native Brazilian orchid Schomburgkia crispa

Agronomy Science and Biotechnology ◽

10.33158/asb.2018v4i2p93 ◽

2018 ◽

Vol 4 (2) ◽

pp. 93

Author(s):

Douglas Bertoncelli ◽

Guilherme Alves ◽

Gustavo Freiria ◽

Felipe Furlan ◽

Helio Neto ◽

...

Keyword(s):

Root Length ◽

Iron Concentration ◽

Leaf Length ◽

In Vitro Cultivation ◽

Nutritional Requirement ◽

Initial Development ◽

Shoot Height ◽

Shoot Number ◽

Number Of Leaves

In vitro cultivation is a highly important biotechnological method widely used for the production of orchid seedlings, but it is necessary to study the suitability of the nutrients used in different kinds of formulation, as the nutritional requirement varies according to the species. The objective was to evaluate different concentrations of iron in the in vitro cultivation of Schomburgkia crispa Lindl seedlings. Seedlings were obtained from seeds germinated in vitro. Modified MS culture medium was used with half of the macronutrient concentration. The micronutrients were added according to the original formulation, except for the iron which was added from a stock solution of FeEDTA (FeSO4.7H2O: 5.6 g L-1 and EDTA: 7.48 g L-1) at 0.0; 2.5; 5.0; 7.5; 10.0 and 12.5mL L-1. At 200 days after seedling transplantation, shoot height, root length, number of leaves, shoot number, leaf length, leaf width, aerial and root dry mass, chlorophyll a, b and carotenoids content were evaluated. A completely randomized design was used, with six treatments and ten replicates of five seedlings. Regression analysis was performed at 5% of significance. The increase in iron concentration caused a reduction in root length and an increase in the number of leaves and shoots. The concentration of 4.13 mL L-1 of FeEDTA was the one that provided the best in vitro growth of S. crispa plants. High concentrations of iron caused a reduction of initial development, but stimulated an increase in the number of shoots.

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Pengendalian Penyakit Pustul Xanthomonas axonopodis pv. glycines Pada Kedelai Dengan Bacillus spp. Asal Filosfer Gulma di Pertanaman Kedelai

Jurnal Pengendalian Hayati ◽

10.19184/jph.v2i2.17141 ◽

2020 ◽

Vol 2 (2) ◽

pp. 53

Author(s):

Siti Rahayu ◽

Suhartiningsih Dwi Nurcahyanti

Keyword(s):

Incubation Period ◽

Infection Rate ◽

Soybean Plant ◽

Xanthomonas Axonopodis ◽

Severity Of Disease ◽

Bacillus Spp ◽

Number Of Leaves ◽

Treatment Application ◽

Number Of Branches

Soybean pustule disease by Xanthomonas axonopodis pv. glycines (Xag) is one of the important diseases of soybean plant. Application Bacillus spp. origin of weed phyllosphere has a great potential as an alternative to control because it is isolated to origin of same region as Xag is the phyllosphere. Research by Nurcahyanti and Ayu obtained the best five isolates of Bacillus spp. of weeds phyllosphere in soybean cropping in inhibiting Xag in vitro. This study used the complete random draft (RAL) 6 treatment, 4 repeats, each unit consists of 4 plants wich treatment application of 5 isolates of Bacillus spp. namely K = control; A = Bacillus JG 1.3; B = Bacillus JG 3.6; C = Bacillus JG 1.4.1; D = Bacillus BGd 1.1; E = Bacillus Bp 2.2. The results showed that application of Bacillus spp. could inhibit Xag in vitro with bacteriostatic mechanism and isolates Bacillus BGd 1.1 has the greatest inhibition of 14.25 mm. Fifth Bacillus spp. can suppress the development of disease and isolates Bacillus BGd 1.1 has best results with the incubation period during 13 HSI, the severity of disease 10.07%, infection rate 0.045 unit/day as well as the effectiveness of control 85.72%. The fifth isolates of Bacillus spp. can not increase the number of leaves but can increase number of branches and isolates Bacillus JG 1.3, Bacillus JG 1.4.1 and Bacillus BGd 1.1 can increase the height of soybean crop.

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Peran Chitosan sebagai Pemacu Pertumbuhan Kultur Anggrek Dendrobium lasianthera J.J.Sm. secara in vitro

JURNAL BIOLOGI PAPUA ◽

10.31957/jbp.1096 ◽

2020 ◽

Vol 12 (1) ◽

pp. 43-49

Author(s):

Verena Agustini ◽

Irma Rahayu ◽

Leonardo A. Numberi ◽

Ziyadatun Ni’mah

Keyword(s):

Positive Influence ◽

Leaf Length ◽

Maximum Effect ◽

Observation Data ◽

Chitosan Concentration ◽

Randomized Design ◽

The Media ◽

Number Of Leaves ◽

Completely Randomized Design

The effect of chitosan on micropropagation of Dendrobium lasianthera J.J.Sm was observed. The media used was Vacin and Went (VW) modified with the addition of coconut water and given chitosan with several concentrations. The study was conducted at the Plant Tissue Culture Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, Cenderawasih University for 6 months, from April to September 2019. Experiments using a Completely Randomized Design (CRD) of 8 treatments with 3 explants in each bottle for total 3 bottle as a replication. The total explants used in this experiment was 72 explants. The concentration of chitosan were 0% , 1%, 5%, 10%, 15%, 20%, 40% and 50% separately. Observation data were analyzed using ANOVA, if there were significant differences followed by DMRT at the 5% level. Observation variables included number and length of roots, number and length of leaves. The results showed that giving chitosan 10% had a maximum effect on the number of roots and number of leaves, 6.67 and 4.67. As for the highest leaf length of 17.43 mm and the longest root length 19.21 mm were in the media with the addition of chitosan concentration of 15%. The observations showed that chitosan had a positive influence on the growth and development of orchids D. lasianthera plantlet in vitro.Keywords : Chitosan, orchid, Vacin and Went, plantlet.

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PENGAPLIKASIAN RADIASI ELEKTROMAGNETIK TERHADAP PERTUMBUHAN VEGETATIF TANAMAN CABAI MERAH (Capsicum Annum L.)

J-PEN Borneo : Jurnal Ilmu Pertanian ◽

10.35334/jpen.v2i2.1509 ◽

2020 ◽

Vol 2 (1) ◽

Author(s):

Bagus Santoso ◽

Amarullah Amarullah ◽

Dwi Santoso

Keyword(s):

Electromagnetic Radiation ◽

Plant Height ◽

Block Design ◽

Leaf Length ◽

Stem Diameter ◽

Capsicum Annum ◽

Randomized Block Design ◽

Number Of Leaves ◽

Screen House ◽

Number Of Branches

ABSTRACT Chili Plants (Capsicum annum L.) originating from the tropics and subtropicsAmericas, especially Colombia, South America, and continue to spread to Latin America. Evidence of chili cultivation was first discovered in Peru's historical siteand leftover seeds that are more than 5000 years old BC in a cave in Tehuacan, Mexico. The spread of chili to the whole world including countries in Asia, like Indonesia carried out by Spanish and Portuguese traders. The purpose of this study was to determine the effect of electromagnetic radiation on the growth and yield of red chilies in the city of Tarakan.This research was conducted from September 2018 to January 2019, at the screen house of the Faculty of Agriculture, Borneo Tarakan University. This study was prepared using factorial Randomized Block Design (RBD) with 5 treatments repeated 5 times. The factors studied were large electromagnetic radiation 56 microtesla with exposure time (P0) 0 minutes, (P1) 20 minutes, (P2) 40 minutes, (P3) 60 minutes, (P4) 80 minutes. Observation parameters; plant height, number of leaves, leaf length, leaf width, stem diameter and number of branches. Analysis of variance using variance ANOVA with a confidence level of 95% and if it is significantly different it will be continued with DMRT further testing.The results of the study show that: 1) Observation parameters that showed statistically significant differences were parameters of plant height, number of branches and number of leaves while parameters that did not show different results were parameters of leaf length, leaf width and stem diameter., 2) The best treatment for each observation parameter is P2 treatment (exposure to electromagnetic radiation for 40 minutes), and the lowest treatment is treatment P0 (without exposure to electromagnetic radiation). Suggestions from this study should be carried out further research on the use of electromagnetic radiation in the vegetative phase until the results of red chili plants (Capsicum annum L.)Keywords: chili, electromagnetics radiation, Randomized Block Design ABSTRAK Tanaman cabai (Capsicum annum L.) berasal dari dunia tropika dan subtropika Benua Amerika, khususnya Colombia, Amerika Selatan, dan terus menyebar ke Amerika Latin. Bukti budidaya cabai pertama kali ditemukan dalam tapak galian sejarah Peru dan sisaan biji yang telah berumur lebih dari 5000 tahun SM di dalam gua di Tehuacan, Meksiko. Penyebaran cabai ke seluruh dunia termasuk negara-negara di Asia, seperti Indonesia dilakukan oleh pedagang Spanyol dan Portugis. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh radiasi elektromagnetik terhadap pertumbuhan dan hasil tanaman cabai merah di kota Tarakan. Penelitian ini dilaksanakan pada tahun 2018, di screen house Fakultas Pertanian Universitas Borneo Tarakan. Penelitian ini disusun dengan menggunakan Rancangan Acak Kelompok (RAK) faktorial dengan 5 perlakuan diulang sebanyak 5 kali. Faktor yang diteliti yaitu besar radiasi elektromagnetik 56 mikrotesla dengan lama pemaparan (P0) 0 menit, (P1) 20 menit, (P2) 40 menit, (P3) 60 menit, (P4) 80 menit. Parameter pengamatan; tinggi tanaman, jumlah daun, panjang daun, lebar daun, diameter batang dan jumlah cabang. Analisis ragam menggunakan sidik ragam Anova dengan tingkat kepercayaan 95% dan apabila berbeda nyata maka akan dilanjut dengan uji lanjut DMRT. Hasil penelitian menunjukkan bahwa: 1) Parameter pengamatan yang menunjukkan hasil berbeda nyata secara statistik adalah parameter tinggi tanaman, jumlah cabang dan jumlah daun sedangkan parameter yang tidak menunjukkan hasil yang berbeda nayata adalah parameter panjang daun, lebar daun dan diameter batang, 2) Perlakuan terbaik untuk setiap parameter pengamatan adalah perlakuan P2 (pemaparan radiasi elektromagnetik selama 40 menit), dan perlakuan terendah adalah perlakuan P0 (tanpa pemaparan radiasi elektromagnetik). Saran dari penelitian ini sebaiknya dilakukan penelitian lanjutan mengenai penggunaan radiasi elektromagnetik pada fase vegetatif sampai hasil tanaman cabai merah (Capsicum annum L.)Kata kunci : cabai merah, radiasi elektromagnetik, Rancangan Acak Kelompok

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Genetic divergence of Nigerian and Indian pearl millet accessions based on agronomical and morphological traits

Journal of Agricultural Sciences Belgrade ◽

10.2298/jas1702115a ◽

2017 ◽

Vol 62 (2) ◽

pp. 115-131

Author(s):

David Animasaun ◽

Joseph Morakinyo ◽

Rama Krishnamurthy ◽

Oba Mustapha

Keyword(s):

Plant Height ◽

Pearl Millet ◽

Morphological Traits ◽

Genetic Relatedness ◽

Agronomic Traits ◽

Crop Improvement ◽

Principal Component ◽

Leaf Length ◽

Leaf Width ◽

Number Of Leaves

The study assessed the genetic diversity of pearl millet accessions grown in Nigeria and India based on morpho-agronomic traits in order to identify genotypes with superior characters which could be utilized in breeding programmes. Twenty-four pearl millet accessions were grown and evaluated for agronomic and morphological traits during the dry and wet seasons of 2015-2016. Data collected on the accessions using standard descriptors were analysed statistically. IP22281 had the highest mean plant height (108.90 cm) while NGB00531 recorded the lowest (61.02 cm). Significant intra-specific variation existed in number of leaves per plant, leaf length, leaf width, number of nodes and internode length, however, stem girth was similar for the accessions. Tillering was generally poor with the highest value (1.60 tillers per plant) found in NGB00531. A significant positive correlation occurred between plant height, number of leaves, leaf length and leaf width. Panicles emerged between 44 and 56 days and NGB00548 had the shortest maturity time. Also, panicle length and peduncle diameter varied significantly for the accessions. The highest grain yield and 1000-grain weight were recorded in NGB00616 and the lowest yield and weight were recorded in IP22269. The principal component analysis grouped the accessions into four clusters, comprising mixtures of Nigerian and Indian members. Similarly, the dendrogram grouped the accessions into two main groups which were sub-divided into smaller clusters with accessions from Nigeria and India in the same cluster. The study concludes that variations in morpho-agronomic and yield characters among the accessions studied could be harnessed for crop improvement. The clustering pattern of these accessions indicated their genetic relatedness, possibly from the same progenitor, but separation by geographical or ecological isolation mechanisms.

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