scholarly journals THE EFFECT OF Streptococcus sp AND Lactobacillus sp ATTACHMENT ON THE LEVEL OF FLUORIDE ION SOLUBILITY IN GIC RESTORATION

2020 ◽  
Vol 5 (1) ◽  
pp. 66
Author(s):  
Rizki Fajar Fauzan ◽  
Muhammad Yanuar Ichrom Nahzi ◽  
Buyung Maglenda

Background: Glass ionomer cement (GIC) is a restoration material that has the ability to release Fluoride ion. The Extrinsic factor that affect the solubility of fluoride is low or acidic pH. Streptococcus sp and Lactobacillus sp are asiduric and acidogenic bacteria which are resistant to acidic conditions and may produce acid. A lower pH (acid) causes higher level of fluoride to be released. Objective: This study was to analyze the effect of Streptococcus sp and Lactobacillus sp attachment on solubility of fluoride level on GIC restoration Method: Twenty-one specimens (5mm diameter x 2mm thickness; n = 7 / group) were fabricated with GIC. The treatment group (Streptococcus sp and Lactobacillus sp) while the control group (Artificial Saliva) was immersion for 7 days in an incubator 37oC. Solubility of fluoride was measured using a pH meter Lutron (208) TL Lutron (Taiwan). Result: One Way Anova and Post Hoc Bonferonni showed that there were significant differences between the treatment groups of Streptococcus sp (5.13 ± 0.86ppm) and Lactobacillus sp (6, 02 ± 0.79ppm) with artificial Saliva group controls (3.03±0,56ppm). Conclusion: There was an effect of Streptococcus sp and Lactobacillus sp bacteria attachment on the solubility of fluoride from the GIC restoration.

e-GIGI ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Fransiska M. Pardosi ◽  
Darmawati A. Indraswari ◽  
Lusiana Batubara ◽  
Nadia Hardini

Abstract: Nanofiller composite resin has small filler size which enhances its properties. However, these properties could decrease due to several factors. Acidic liquid such as coffee could reduce the hardness of composite resin. This study was aimed to analyze the effect of robusta and arabica coffee immersion on the hardness of nanofiller composite resin. A total of 27 composite resin samples were used and then were polished by using a soflex disc for 30 seconds on each roughness level. Samples were then divided into three groups, as follows: the control group with artificial saliva and the treatment groups with robusta coffee and arabica coffee. The composite resin molds were immersed in the three groups for 5 days. After five days of immersion, the hardness of the sample was tested by using a Vickers hardness tester. Artificial saliva as the control group had the highest mean hardness value of 112.98±8.67 VHN, followed by robusta coffee, and then by arabica coffee. The One Way Anova and post hoc LSD test showed that there were significant differences in all groups (p<0.05) except for the two treatment groups, namely robusta and arabica coffee groups which did not show any significant difference in the resin hardness (p>0.05). In conclusion, robusta and arabica coffee affect the hardness of the nanofiller composite resin, but there is no significant difference in the hardness between the robusta and arabica coffee groups.Keywords: composite resin hardness, nanofiller, robusta coffee, arabica coffee Abstrak: Resin komposit nanofiller memiliki ukuran filler kecil yang dapat meningkatkan fungsinya namun fungsi tersebut dapat menurun oleh beberapa hal. Cairan asam yang dikonsumsi seperti kopi dapat menurunkan kekerasan resin komposit. Penelitian ini bertujuan untuk mengetahui pengaruh perendaman kopi robusta dan arabika terhadap kekerasan resin komposit nanofiller. Sampel resin komposit yang digunakan sebanyak 27 buah dan dilanjutkan dengan pemolesan menggunakan soflex disc selama 30 detik setiap tingkat kekasarannya. Sampel kemudian dibagi dalam tiga kelompok, yaitu kelompok kontrol dengan saliva buatan dan dua kelompok perlakuan dengan kopi robusta dan arabika. Sebanyak 27 buah resin komposit direndam ke dalam tiga kelompok tersebut selama lima hari kemudian diuji kekerasannya dengan vickers hardness tester. Saliva buatan sebagai kelompok kontrol memiliki nilai rerata kekerasan tertinggi sebesar 112,98±8,67 VHN, diikuti kopi robusta, dan nilai terendah yaitu kopi arabika. Hasil uji One Way Anova dan post hoc LSD menunjukkan terdapat perbedaan bermakna pada semua kelompok (p<0,05) kecuali pada kedua kelompok perlakuan yaitu antara kopi robusta dan arabika tidak menunjukkan adanya perbedaan kekerasan yang bermakna (p>0,05). Simpulan penelitian ini ialah kopi robusta dan arabika memengaruhi kekerasan resin komposit nanofiller namun tidak terdapat perbedaan kekerasan antara keduanya.Kata kunci: kekerasan resin komposit, nanofiller, kopi robusta, kopi arabika


2019 ◽  
Vol 56 (3) ◽  
pp. 548-553
Author(s):  
Zsigmond Lorand Makkai ◽  
Melinda Szekely ◽  
Zita Fazakas ◽  
Levente Mester Nagy ◽  
Kinga Matyas Barta ◽  
...  

Dental caries and secondary caries are the most common problems in dentistry. Many studies provided evidence for the anticariogenic effect of fluorides. The incidence of secondary caries is decreased in teeth restored with fluoride releasing materials. The purpose of the present in vitro study is to evaluate the fluoride release and uptake capability of two glass-ionomer cements and two compomers. A total number of 60 specimens were prepared according to the manufacturer�s instructions using silicone molds. The specimens were transferred in plastic containers containing artificial saliva (pH=7), incubated at 37�C for 24 h and divided in three subgroups: control group, varnish treated group and dentifrice treated group. The fluoride ion content was measured on five times during a period of 30 days using an Orion 720A fluoride meter with 9609 BN fluoride ion electrode. The self-curing glass-ionomer cement released the highest amounts of fluoride ions at the beginning and after the one month study period. The samples of the four materials tested in the varnish treated groups showed significant differences compared to their control groups on the second day. Dentifrice treated groups did not show significant differences compared to their control groups, except values measured after 30 days. Application of fluoride varnish produced an increase of fluoride release for the first 24 h in case of all materials. However, results suggest that the use of fluoridated dentifrice would be more effective in caries prevention due to their fluoride uptake capability.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Philipp Körner ◽  
Luca Georgis ◽  
Daniel B. Wiedemeier ◽  
Thomas Attin ◽  
Florian J. Wegehaupt

Abstract Background This in-vitro-study aimed to evaluate the potential of different fluoride gels to prevent gastroesophageal reflux induced erosive tooth wear. Methods Surface baseline profiles of a total of 50 bovine enamel specimens [randomly assigned to five groups (G1–5)] were recorded. All specimens were positioned in a custom made artificial oral cavity and perfused with artificial saliva (0.5 ml/min). Reflux was simulated 11 times a day during 12 h by adding HCl (pH 3.0) for 30 s (flow rate 2 ml/min). During the remaining 12 h (overnight), specimens were stored in artificial saliva and brushed twice a day (morning and evening) with a toothbrush and toothpaste slurry (15 brushing strokes). While specimens in the control group (G1) did not receive any further treatment, specimens in G2–5 were coated with different fluoride gels [Elmex Gelée (G2); Paro Amin Fluor Gelée (G3); Paro Fluor Gelée Natriumfluorid (G4); Sensodyne ProSchmelz Fluorid Gelée (G5)] in the evening for 30 s. After 20 days, surface profiles were recorded again and enamel loss was determined by comparing them with the baseline profiles. The results were statistically analysed using one-way analysis of variance (ANOVA) followed by Tukey`s HSD post-hoc test. Results The overall highest mean wear of enamel (9.88 ± 1.73 µm) was observed in the control group (G1), where no fluoride gel was applied. It was significantly higher (p < 0.001) compared to all other groups. G2 (5.03 ± 1.43 µm), G3 (5.47 ± 0.63 µm, p = 0.918) and G4 (5.14 ± 0.82 µm, p > 0.999) showed the overall best protection from hydrochloric acid induced erosion. Enamel wear in G5 (6.64 ± 0.86 µm) was significantly higher compared to G2 (p = 0.028) and G4 (p = 0.047). Conclusions After 20 days of daily application, all investigated fluoride gels are able to significantly reduce gastroesophageal reflux induced loss of enamel.


2018 ◽  
Vol 47 ◽  
pp. 07006
Author(s):  
Saryono Saryono ◽  
Hesti Devinta ◽  
Abdul Haris Budi Widodo ◽  
Arif Imam Hidayat

Contaminated water often affects the occurrence of periodontitis in the coastal area. The diabetic hypercholesterolemia-induced periodontitis in Indonesia is very high. The use of coenzyme Q10 to treat this disease has never been investigated yet. Therefore, this study aimedto analyze the effect of coenzyme Q10 on the lipid profile of diabetic hypercholesterolemia-induced periodontitis.Twenty four rats were randomized into 6 groups (G1-G6). The groups (G1-G3) are healthy, negative and positive control group respectively. The treatment groups (G4-G6) are diabetic hypercholesterolemia-induced rats given coenzyme q10 dose of 13,5; 27 and 54 mg/kg respectively. Rats were induced by periodontitis, hypercholesterolemia and diabetes mellitus. Coenzyme Q10 was administered orally using 2 mL gastric tube once a day for 14 days. Lipid profile including triglycerides, HDL, and atherogenic index (IA) was measured enzymatically by the CHOD-PAP method. Data were analyzed by one-way ANOVA test and followed by the Least Significant Difference (LSD) post hoc test.Coenzyme Q10 with a dose of 54 mg/kgis effective in lowering triglyceride, and atherogenic index and increasing HDL level in diabetic hyperlipidemia-induced periodontitis rats model. This research supports the potential effects of coenzyme Q10 supplementation to improve lipid profile in diabetic hypercholesterolemia-induced periodontitis in the coastal area.


2018 ◽  
Vol 27 (4) ◽  
pp. 372-377 ◽  
Author(s):  
Muhammad Ali Faridi ◽  
Abdul Khabeer ◽  
Saad Haroon

Objectives: Glass ionomer cement (GIC) is routinely placed as a restorative material in dentistry. However, due to its poor physical properties, its use is limited to cases where the level of stress on restoration is minimal. Improved formulations of GIC have been developed to overcome these drawbacks. The purpose of this study was to evaluate flexural strength of a conventional GIC (Fuji IX) against a newly developed glass carbomer cement (GCP). Materials and Methods: For Fuji IX and GCP, a total of 80 blocks were prepared and divided into 16 groups (n = 5). These groups were further categorized according to the storage medium (artificial saliva and Vaseline) and time intervals (24 h and 1, 2, and 4 weeks). A 3-point bending test was carried out, and statistical analysis was done using ANOVA and Tukey post hoc tests. Results: Fuji IX showed a mean flexural strength of 25.14 ± 13.02 versus 24.27 ± 12.57 MPa for GCP. There was no significant statistical difference between both materials when compared under storage media. Both materials showed the highest value for flexural strength at 2 weeks of storage and lowest at 4 weeks. Conclusion: The storage media do not affect the flexural strength of the specimens with reference to time. Time is the unique factor with relative influence on mean resistance to fracture. Further testing is required to evaluate the true potential of the newly developed GCP.


2005 ◽  
Vol 16 (2) ◽  
pp. 103-106 ◽  
Author(s):  
Rogério de Oliveira ◽  
Adriana Franco Paes Leme ◽  
Marcelo Giannini

This in vitro study evaluated the surface microhardness of human enamel submitted to bleaching with 10% carbamide peroxide (CP) containing calcium or fluoride. Ninety-eight dental blocks (5 x 5 mm²) with polished enamel surfaces were randomly assigned to 7 treatment groups (n=14), as follows: without bleaching and storage in artificial saliva (control); 10% CP; 10% CP + 0.05% calcium; 10% CP + 0.1% calcium; 10% CP + 0.2% calcium; 10% CP + 0.2% fluoride; and 10% CP + 0.5% fluoride. During 14 days, enamel surfaces were daily exposed to a 6-h bleaching regimen followed by storage in artificial saliva. Surface microhardness was measured before (baseline), during (7th day), immediately after bleaching (14th day) and 1 week post bleaching. Data were analyzed by two-way ANOVA and Tukey's test (p<0.05). All treatments reduced SM significantly during the bleaching cycle (7th day), immediately after bleaching (14th day) and 1 week post bleaching, compared to baseline and to the unbleached control group. In conclusion, in spite of the addition of calcium and fluoride, all bleaching treatments affected the enamel surface microhardness.


2017 ◽  
Vol 1 ◽  
Author(s):  
Christandi Prana Yuwana ◽  
Christnawati Christnawati ◽  
Cenderawasih Farmasyanti

<p class="AbstractContent"><strong>Objective</strong>: To study the effects of immersion time in three kinds of carbonated beverages on orthodontic elastic latex’s tensile strength</p><p class="AbstractContent"><strong>Methods</strong>: Subjects consist of 32 specimens orthodontic elastic latex ¼" and 4.5 oz of power, divided into 8 groups (IA, IB, IC, ID, IIA, IIB, IIC, and IID). Each group are immersed in artificial saliva (pH7) and stored in incubator (37 °C) for 24hrs for group I and 48hrs for group II. Each treatment groups will be immersed in three different carbonated beverages for 90s per day. Elastics being measured its tensile strength for group I at 24th hour, and 48th hour for group II using tension gauge. The data was tested with two way anova and post hoc Tukey.</p><p class="AbstractContent"><strong>Results:</strong> The result shows immersion time decreases elastic latex’s tensile strength (<em>p</em> &lt; 0.05), and carbonated beverages affect elastic latex’s tensile strength (<em>p</em> &lt; 0.05).</p><p class="AbstractContent"><strong>Conclusion:</strong> (1) There is a difference of orthodontic elastic latex tensile strength in the immersion in carbonated beverages for 24 and 48 hours, (2) there is a difference of elastic latex tensile strength orthodontic in the immersion in three kinds of carbonated beverages, and (3) there is interaction between immersion time in carbonated beverages and carbonated beverage brands</p>


2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract hassubstantial properties such as albumin, Zn,Cu, and Feto accelerate wound healing. The purpose of this study is to assessthe effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. Thisstudy was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatmentgroups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, eachtreatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of eachgroup was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant differencebetween negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increasedneocapillaries count in wound healing process.


Author(s):  
Warobi Warobi ◽  
Delima Engga Maretha ◽  
Asnilawati Asnilawati ◽  
Mashuri Masri

Air fresheners are products that contain chemicals aimed at reducing unpleasant odours in confined spaces. The use of synthetic air fresheners turns out to harm health because some of their leased Volatile Organic Compounds are classified as toxic compounds and are carcinogens. Air freshener enters the body through the inhalation process in the respiratory system. Modern air fresheners are available in liquid (aerosol) and gel forms. Air fresheners contain addictive substances and solvents such as 1,4-dichlorobenzene which can affect pulmonary function. The purpose of this study is to know the effect of exposure to liquid air freshener on the histology of bronchi of mice (Mus musculus). This study used 20 male mice (Mus musculus) consisting of 4 treatments with 5 replications. The research design is true experimental in the form of a post-test only control group with Completely Randomized Design (CRD). The post-test was done by observing the histological picture of the mice's bronchi after exposure to liquid air freshener given 3x/day for a period of P1 = 2 weeks, P2 = 4 weeks and P3 = 6 weeks. Quantitative data on bronchial histology was tested using the One Way ANOVA statistical test followed by the Post Hoc Tukey test. The results of the study found changes in the histology of the bronchi, thickening of the epithelial tissue of mice. Analysis of comparative data between the control and treatment groups statistically obtained p = 0.010 <0.05, meaning that there was a significant effect and change in the bronchial tubes exposed to liquid air freshener. Histologically there were differences in histology between the control and treatment groups. It was concluded that liquid air freshener had a significant effect on the histological picture of the mice's bronchi.


2016 ◽  
Vol 2 (2) ◽  
pp. 60
Author(s):  
Devi Puspita Handayani ◽  
Dewi Puspitasari ◽  
Nurdiana Dewi

The effect of immersion of decoction water of Red Betel Leaf (Piper crocatum) on the surface hardness of composite resin. The purpose of this study was to determine the effect of immersion of decoction water of red betel leaf on the surface hardness of composite resin compared to alcoholic mouth rinse. It was a pure experimental study with post-test only with control group design. The total samples were 27 samples divided into 2 treatment groups and 1 control group, each of which consisted of 9 samples. The treatment groups were immersed in the decoction water of red betel leaf and alcoholic mouth rinse. The control group was immersed in aquadest. After the immersion, the samples were measured using Vickers Micro hardness Tester. Analysis with one way anova and post hoc Bonferroni showed a significant difference (p<0.05) on the surface hardness of composite resin after being immersed in decoction water of red betel leaf(79,81±3,76) kg/mm2 and alcoholic mouth rinse (67,11±2,51) kg/mm2. Based on this research, it can be concluded that there was an effect of immersion of decoction water of red betel leaf if compared with alcoholic mouth rinse. The value of surface hardness of composite resin immersed in alcoholic mouth rinse was lower than the decoction water of red betel leaf.ABSTRAKPenelitian ini bertujuan untuk mengetahui efek perendaman dalam rebusan daun sirih merah terhadap kekerasan permukaan resin komposit bila dibandingkan dengan obat kumur beralkohol. Penelitian ini merupakan penelitian eksperimental murni dengan post-test only with control group design. Jumlah sampel sebanyak 27 yang dibagi dalam 2 kelompok perlakuan dan 1 kelompok kontrol dengan masing-masing kelompok sebanyak 9 sampel. Kelompok perlakuan direndam dengan air rebusan daun sirih merah dan obat kumur beralkohol. Kelompok kontrol direndam dengan akuades steril. Setelah itu dilakukan pengukuran menggunakan Vickers Microhardness Tester. Hasil uji One Way Anova dan Post Hoc Bonferroni menunjukkan bahwa terdapat perbedaan bermakna nilai kekerasan permukaan resin komposit yang direndam dengan air rebusan daun sirih merah (79,81±3,76) kg/mm2 dan obat kumur beralkohol (67,11±2,51) kg/mm2 dengan nilai kemaknaan (p<0,05). Berdasarkan penelitian yang dilakukan dapat diambil kesimpulan bahwa terdapat efek lebih rendah pada perendaman dalam rebusan daun sirih merah terhadap kekerasan permukaan resin komposit bila dibandingkan dengan obat kumur beralkohol. Nilai kekerasan permukaan resin komposit yang direndam obat kumur beralkohol lebih rendah dibandingkan air rebusan daun sirih merah.


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