PENGARUH BERBAGAI ZAT PENGATUR TUMBUH (ZPT) TERHADAP PERTUMBUHAN STEK BATANG PULAI RAWA (Alstonia spatulata)

2021 ◽  
Vol 4 (2) ◽  
pp. 198
Author(s):  
Winda Ningsih Pardede ◽  
Gusti Muhammad Hatta ◽  
Damaris Payung
Keyword(s):  
Root Growth ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Growth Response ◽  
Stem Cuttings ◽  
The Third ◽  
Randomized Design ◽  
Plant Stem ◽  
Completely Randomized Design ◽  
Bean Sprouts

Some of the goals that exist in this research are to analyze how the effect of giving Rootone F, giving Rootmost and giving Bean Sprouts Extract to the growth response of stem cuttings in Pulai Rawa (Alstonia spatulata. Growth Regulatory Substances used in this study there are 3 types of treatments such as giving Rootone F as the first treatment, the second treatment is by giving rootmost and by giving Bean Sprouts Extract as the third treatment and in this study there is control as one of the comparison between the differences between Stem cuttings that use Growth Regulatory Substances with no provision of ZPT. In November 2019 until January 2020 this study took place in the shade house of the forestry faculty at Lambung Mangkurat University.  RAL (Completely Randomized Design) The method used in this study is to use with a set of 4 treatments that were repeated 20 times in each treatment, then there were 80 experimental units in this study.  The results obtained in this study showed the effect of various growth regulators which differed to the response of the growth of Pulai Rawa plant stem cuttings.  The provision of rootone F did not affect the growth response while the administration of Growth Regulator Substance Rootmost gave an effect on the response of root growth of Pulai Rawa cuttings (Alstonia spatulata) and by giving Bean Sprout Extract which had an influence on the growth in number of cuttings.Keywords : Growth regulators; Stem cuttings; Pulai Rawa

scholarly journals ROOT INDUCTION OF PATCHOULI PLANT STEM CUTTINGS USING IBA GROWTH REGULATOR ON SEVERAL PLANT MEDIA

2021 ◽  
Vol 1 (1) ◽  
pp. 59-65
Author(s):  
Rahadian Yamin ◽  
Irwan Mahakam Lesmono Aji ◽  
Muhamad Husni Idris
Keyword(s):  
Growth Regulators ◽  
Growth Regulator ◽  
Growth Parameters ◽  
Raw Material ◽  
Root Induction ◽  
Stem Cuttings ◽  
Media Composition ◽  
Study Program ◽  
Randomized Design ◽  
Cultivation Techniques

Patchouli (Pogostemon cablin Benth.) plant is one of the essential oil producing plants, that is useful in the chemical industry as a raw material for fragrance products and in the pharmaceutical field. The success rate of patchouli early growth, is largely determined by the cultivation techniques used, including the use of growth regulators, and growing media. This study aims to determine the effect of IBA growth regulators and the composition of plant media on the growth of patchouli plants. This research is carried out in the Greenhouse of the Forestry Study Program of the University of Mataram, using a Factorial Completely Randomized Design (RALF), the first factor is IBA growth regulator with 3 levels, namely 0, 20, 25 ppm and the second factor is composition of plant media with 3 levels, namely forest soil media, soil media:sand:compost (1:1:1), and soil media:sand:compost (2:1:3). The results show that the effect of IBA significantly affect the success percentage of cuttings and the number of roots of patchouli plants. The effect of plant media composition and the interaction between treatment of IBA with plant media composition did not have a significant effect on the growth parameters of patchouli plants.

scholarly journals Induction of Barangan Banana shoot (Musa acuminata L.) from North Nias through Tissue Culture by giving 2,4-D and Kinetin

2016 ◽  
Vol 1 (2) ◽  
pp. 1
Author(s):  
Destarius Zebua ◽  
Suci Rahayu ◽  
Saleha Hanum
Keyword(s):  
Tissue Culture ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Musa Acuminata ◽  
Basal Part ◽  
Banana Weevil ◽  
Shoot Induction ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Basal Position

The research on induction of banana barangan shoot (Musa acuminata L.) from North Nias through tissue culture by giving 2,4-D and kinetin was conducted in the Laboratory of Tissue Culture  University of North Sumatera from May until October 2014. The main objective of this research was to know the explants of banana weevil in apical and basal position with growth regulator 2,4-D with kinetin which are able to induce shoots from callus initials. On this research, the treatment tested for induction of shoots was growth regulators 2,4-D in the concentration 0 mg/L, 1 mg/L, 1,5 mg/L, 2 mg/L and 2,5 mg/L with growth regulators kinetin in the concentration 0 mg/L, 5 mg/L, 6 mg/L, 7 mg/L and 8 mg/L. The research was designed using Completely Randomized Design (CRD) two factorial with repetition of experiment. The results of research showed that the interaction between concentration of 2,5 mg/L 2,4-D with 5 mg/L kinetin was fastest (79 days) forming the shoot derived from explant basal part. The concentration of 2,5 mg/L 2,4-D with 5 mg/L kinetin produced the average number of shoots formed for 3.00 and average number of highest shoots for 1.50 cm. Keywords: Musa acuminata, weevil, initiation, callus, shoot, induction, barangan banana, 2,4-D, kinetin.

scholarly journals Penggunaan Air Kelapa Untuk Setek Batang Jati (Tectona Grandis)

2016 ◽  
Vol 4 (1) ◽  
pp. 61
Author(s):  
Rega Renvillia ◽  
Afif Bintoro ◽  
Melya Riniarti
Keyword(s):  
Growth Regulator ◽  
Water Immersion ◽  
Tectona Grandis ◽  
Stem Cutting ◽  
Coconut Water ◽  
Stem Cuttings ◽  
Randomized Design ◽  
Root Cutting ◽  
Water Cutting ◽  
Completely Randomized Design

Teak plant wasa treewhich timber has durability classI and strong class I.  It hasmany benefits, from roots to leaves. Variousbenefits of teak causing to do cultivation, one of propagation activities that often carried out was vegetative. Vegetative propagation of plants can be done bystem cutting, root cutting and shoot cutting.  Using of growth regulator with coconut water immersion is expected to spur the growth rootofteak stem cutting. This study aimed to determine the effect of using coconut water to increase the success of teak stem cuttinggrowth, andto determine the best dose of using coconut water forteak stem cutting growth. This treatment used Completely Randomized Design(CRD).  This research consisted of five treatments that usedcoconut water treatment 0%, 25%, 50%, 75%, and 100%. Each treatmentconsisted of five teak stem cuttings with repetition as much as 5 times.  Amount ofmaterial plants required 5 x 5 x 5 = 125 teak stem cuttings.  The resultshowed by using coconut wateras a growth regulator could increasethe growth of teak (Tectona grandis) stem cuttingin 50%--100% concentration and the best dose of coconut water asa growth regulator was 100%.  Keywords : coconut water, cutting, dose, growth regulator, teak

scholarly journals RESPON PERTUMBUHAN STEK BATANG VANILI (Vanilla planifolia) TERHADAP LAMA PERENDAMAN ZAT PENGATUR TUMBUH ROOT MOST

AGRICA ◽  
2019 ◽  
Vol 12 (1) ◽  
pp. 43-58
Author(s):  
Wilhelmus Haman ◽  
Kristono Yohanes Fowo
Keyword(s):  
Root Growth ◽  
Root Length ◽  
Shoot Growth ◽  
Dry Weight ◽  
Shoot Length ◽  
Stem Cuttings ◽  
Soaking Time ◽  
Randomized Design ◽  
Wet Weight ◽  
Completely Randomized Design

This study aims to determine the response of the immersion time of vanilla stem cuttings in the most root growth regulating agent and to know the length of soaking time of the vanilla stem cuttings in the most optimum root growth regulator. The design used in this study was a Completely Randomized Design (CRD) with six treatments soaking in minutes. Repeated four times, namely P1 (10 minutes), P2 (20 minutes), P3 (30 minutes), P4 (40 minutes), P5 (50 minutes), P6 (60 minutes). Variables observed including the number of days of first shoot growth, the number of roots, root length, shoot diameter, shoot length, wet weight of cuttings, and dry weight of cuttings.The results showed that the treatment of P6 gave a very significant effect. On the variable growth in the number of roots (55.59%), root length (58.86%), number of days of first shoot growth (28 HST), shoot diameter (70.15%) , shoot length (71.43%), wet weight of cuttings (37.14%) and dry weight of cuttings (65.04%) at each age of observation.

Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus
Keyword(s):  
Data Analysis ◽  
Analysis Of Variance ◽  
Research Method ◽  
Moringa Oleifera ◽  
The Third ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design ◽  
Second Period

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

THE SOURCES OF AUXIN HORMONE TO GROWTH OF SHOOT PINEAPPLE STEM CUTTING (Ananascomosus.L. Merr.)

2016 ◽  
Vol 1 (1) ◽  
pp. 45-52
Author(s):  
Palupi Puspitorini
Keyword(s):  
Water Concentration ◽  
Urine Concentration ◽  
Experimental Results ◽  
Stem Cuttings ◽  
Randomized Design ◽  
Natural Hormone ◽  
Cow Urine ◽  
Urine Concentrations ◽  
Completely Randomized Design ◽  
Better Than

The aim of this study was to select the best sources of auxin of which it can stimulate the growth of shoots Pineapple plant cuttings. This research is compiled in a completely randomized design (CRD) with 4 treatments and 6 replications. The Data were statistically Analyzed by the DMRT. Level of treatment given proves that no treatment 0%, cow urine concentration of 25%, young coconut water concentration of 25% and Rootone F 100 mg / cuttings. The results showed that cow urine concentrations of 25% and Rootone F 100 mg give the best results in stimulating the growth of shoots pineapple stem cuttings. Experimental results concluded that the effect of this natural hormone were better than the shoots without given hormone.           

scholarly journals Effect of Storage Temperature on Beauveria bassiana (Bals.) Vuill. Viability on Several Carriers

2006 ◽  
Vol 22 (1) ◽  
Author(s):  
Sri Sukamto
Keyword(s):  
Beauveria Bassiana ◽  
Storage Temperature ◽  
Ultra Violet ◽  
Rice Flour ◽  
Plant Diseases ◽  
Maize Flour ◽  
The Third ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tapioca Flour

One of the entomopatogenous fungus types commonly observed and showed potency as myco-insectiside is Beauveria bassiana(Bals.) Vuill.In order to support effectiveness and patogenous activity of B. bassiana, it is necessary to add a carrying agent that protects its spores from ultra violet ray. This study aims to investigate the effect of storage temperature on viability of B. Bassianaspores on the carrier material. The observation was carried out in the Laboratory of Plant Diseases, Indonesian Coffee and Cocoa Research Institute. The research was arranged in completely randomized design by three factors. The first factor was carrier (C), that consists of C1 = rice flour, C2 = maize flour and C3 = tapioca flour. The second factor was dosage (D), that consists of D1 = 1 g B. bassiana+ 1 g carrier; D2 = 1 g B. bassiana+ 5 g carrier and D3 = 1 g B. bassiana+ 10 g carrier. The third factor was temperature of the storage (T),that consists of T1 = 5oC; T2 = 23oC and T3 = 29oC. Viability of B. Bassiana spores was examined by observing development of 100 blastopores randomly and determined under light microscope with 400 times magnification. Observation was conducted in two replicates after the spores of B. bassiana were kept in the storage for 2, 4, 8 and 16 weeks. The result showed that by adding 1 g tapioca flour and temperature of storage of 5oC was potentiall in keeping viability of B. bassianaspores at least for 2 months. It was due to that tapioca flour gave better effect than rice and maize flours in keeping the storage and appropriate low temperature. Viability of B. bassianaspores decreased with increasing carrier dosage, temperature and duration of the storage. Whereas, storage at 5oC was found to be a better condition in keeping viability of dry pure B. bassianaspores longer than conditions of 23o and 29oC. Key word:Beauveria bassiana, temperature, viability,carrier.

scholarly journals Effects of the growth regulators for the induction of somatic embryos from explants in an endemic and threatened Echinocactus parryi Engelm.

2021 ◽  
Vol 27 (3) ◽  
pp. 431-447
Author(s):  
Dolores Adilene García-González ◽  
◽  
María del Socorro Santos-Díaz ◽  
Juan Pedro Flores-Margez ◽  
Pedro Osuna-Ávila ◽  
...  
Keyword(s):  
Growth Regulators ◽  
Somatic Embryos ◽  
Histological Analysis ◽  
Globular Stage ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Green Callus ◽  
Dichlorophenoxy Acetic Acid ◽  
Mature Seeds

Introduction: The list of threatened species is enhancing and needs to be revised by integrating plant tissue culture tools with conventional techniques that support the appropriate management of these species. Objective: To assess the effects of the growth regulators for the induction of somatic embryos from mature seeds, shoots, and compact green callus of Echinocactus parryi Engelm. and the histological analysis of the embryogenic structures. Materials and methods: A completely randomized design was utilized to evaluate three types of explants (apical, medium, and basal) cultured on basal Murashige & Skoog media (MS) with different growth regulators concentrations (2, 4-D [dichlorophenoxy acetic acid], BAP [6-benzylaminopurine] and kinetin, at four levels: 0.5, 1, 1.5, and 2 mg∙L -1 ). Histological analysis of the embryogenic structures was performed. Results and discussion: The 2, 4-D induced both embryogenic and organogenic callus from seeds and shoot explants. The globular stage did not evolve to their maturity, presumably because of 2, 4-D accumulation. The compact callus explants were the more efficient to induce 19.2 somatic embryos per explant when they were cultured in the medium with 0.5 mg∙L -1 kinetin. However, the latest phases did not germinate, probably due to abnormalities generated by genetic and epigenetic changes in the DNA that can cause abnormal somatic embryos. The histology image demonstrated that the globular and torpedo structures were visible under a microscope showing stained nucleus and numerous starch grains. Conclusions: E. parryi is a species that can produce a high number of embryogenic structures, which represents a great potential to grow massive plants.

scholarly journals Respon Pertumbuhan Indigofera (Indigofera zollingeriana) yang Diberikan Pupuk Kotoran Kelinci dan Biochar

2021 ◽  
Vol 2 (1) ◽  
pp. 19-26
Author(s):  
Gresy Eva Tresia ◽  
A. Saenab
Keyword(s):  
Growth Response ◽  
Chicken Manure ◽  
Marginal Land ◽  
Stem Height ◽  
Randomized Design ◽  
Increase Productivity ◽  
Number Of Leaves ◽  
Indigofera Zollingeriana ◽  
Completely Randomized Design ◽  
Rabbit Manure

Application of organic manures for improvement soil fertility is expected to increase productivity of Indigofera zollingeriana in marginal land agro-ecosystems.The aim of this study was to evaluate the growth response of Indigofera zollingeriana on application of rabbit and biochar fertilizers. This study used a completely randomized design with 3 treatments and 10 replications. The treatments consisted of T0 (chicken manure biofertilizer as control), T1 (biochar), and T2 (biochar + rabbit manure biofertilizer). The variables observed were stem height (cm), number of stalks, and number of leaves. Application of rabbit manure biofertilizer and biochar (T2) gave the highest plant height, number of stalks and leaves Indigofera of 45.68±4.18 cm, 35.80±1.03 stalk, and 128.5±11.65 leaves, respectively. Also, application T2 enhanced growth vegetative Indigofera started at 7 weeks of age (35th days after planting). It could be concluded that the combination of biochar and rabbit manure biofertilizer gives the optimal result in the growth of Indigofera.

scholarly journals Soil characteristic and shallot growth with gypsum and zeolite amendments in irrigated saline Alfisol and Inceptisol

2021 ◽  
Vol 8 (3) ◽  
pp. 2801-2808
Author(s):  
Rahayu Rahayu ◽  
Jauhari Syamsiyah ◽  
Livia Dewi
Keyword(s):  
Electric Conductivity ◽  
N Uptake ◽  
Sodium Adsorption Ratio ◽  
Pot Experiment ◽  
Soil Types ◽  
Soil Characteristic ◽  
Saline Irrigation ◽  
The Third ◽  
Randomized Design ◽  
Completely Randomized Design

Salinity of soil and irrigation is a factor that may cause a decrease in shallot productivity, so it requires efforts with amendments. This research aimed to determine the effect of gypsum and zeolite amendments on soil and shallot growth with saline irrigation. A pot experiment was conducted in the field using a completely randomized design with three factors. The first factor was two soil types (Alfisol and Inceptisol); the second factor was three shallot cultivars (Brebes, Purbalingga, and Pemalang); and the third factor was two types of soil amendments. The results showed that gypsum and zeolite reduced pH, sodium adsorption ratio (SAR), electric conductivity paste (ECp) and Na of the soils studied. Gypsum and zeolite increased the uptake of N, P and K by shallot plants. The increase of N uptake by applying gypsum on Inceptisol was more effective to Brebes and Purbalingga cultivars than Pemalang cultivar. Gypsum increased the diameter and number of bulbs in Inceptisol. Zeolite and gypsum increased bulb weight of Purbalingga cultivar in Alfisol.

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