scholarly journals Soil characteristic and shallot growth with gypsum and zeolite amendments in irrigated saline Alfisol and Inceptisol

2021 ◽  
Vol 8 (3) ◽  
pp. 2801-2808
Author(s):  
Rahayu Rahayu ◽  
Jauhari Syamsiyah ◽  
Livia Dewi
Keyword(s):  
Electric Conductivity ◽  
N Uptake ◽  
Sodium Adsorption Ratio ◽  
Pot Experiment ◽  
Soil Types ◽  
Soil Characteristic ◽  
Saline Irrigation ◽  
The Third ◽  
Randomized Design ◽  
Completely Randomized Design

Salinity of soil and irrigation is a factor that may cause a decrease in shallot productivity, so it requires efforts with amendments. This research aimed to determine the effect of gypsum and zeolite amendments on soil and shallot growth with saline irrigation. A pot experiment was conducted in the field using a completely randomized design with three factors. The first factor was two soil types (Alfisol and Inceptisol); the second factor was three shallot cultivars (Brebes, Purbalingga, and Pemalang); and the third factor was two types of soil amendments. The results showed that gypsum and zeolite reduced pH, sodium adsorption ratio (SAR), electric conductivity paste (ECp) and Na of the soils studied. Gypsum and zeolite increased the uptake of N, P and K by shallot plants. The increase of N uptake by applying gypsum on Inceptisol was more effective to Brebes and Purbalingga cultivars than Pemalang cultivar. Gypsum increased the diameter and number of bulbs in Inceptisol. Zeolite and gypsum increased bulb weight of Purbalingga cultivar in Alfisol.

Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus
Keyword(s):  
Data Analysis ◽  
Analysis Of Variance ◽  
Research Method ◽  
Moringa Oleifera ◽  
The Third ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design ◽  
Second Period

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

scholarly journals Effect of Storage Temperature on Beauveria bassiana (Bals.) Vuill. Viability on Several Carriers

2006 ◽  
Vol 22 (1) ◽  
Author(s):  
Sri Sukamto
Keyword(s):  
Beauveria Bassiana ◽  
Storage Temperature ◽  
Ultra Violet ◽  
Rice Flour ◽  
Plant Diseases ◽  
Maize Flour ◽  
The Third ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tapioca Flour

One of the entomopatogenous fungus types commonly observed and showed potency as myco-insectiside is Beauveria bassiana(Bals.) Vuill.In order to support effectiveness and patogenous activity of B. bassiana, it is necessary to add a carrying agent that protects its spores from ultra violet ray. This study aims to investigate the effect of storage temperature on viability of B. Bassianaspores on the carrier material. The observation was carried out in the Laboratory of Plant Diseases, Indonesian Coffee and Cocoa Research Institute. The research was arranged in completely randomized design by three factors. The first factor was carrier (C), that consists of C1 = rice flour, C2 = maize flour and C3 = tapioca flour. The second factor was dosage (D), that consists of D1 = 1 g B. bassiana+ 1 g carrier; D2 = 1 g B. bassiana+ 5 g carrier and D3 = 1 g B. bassiana+ 10 g carrier. The third factor was temperature of the storage (T),that consists of T1 = 5oC; T2 = 23oC and T3 = 29oC. Viability of B. Bassiana spores was examined by observing development of 100 blastopores randomly and determined under light microscope with 400 times magnification. Observation was conducted in two replicates after the spores of B. bassiana were kept in the storage for 2, 4, 8 and 16 weeks. The result showed that by adding 1 g tapioca flour and temperature of storage of 5oC was potentiall in keeping viability of B. bassianaspores at least for 2 months. It was due to that tapioca flour gave better effect than rice and maize flours in keeping the storage and appropriate low temperature. Viability of B. bassianaspores decreased with increasing carrier dosage, temperature and duration of the storage. Whereas, storage at 5oC was found to be a better condition in keeping viability of dry pure B. bassianaspores longer than conditions of 23o and 29oC. Key word:Beauveria bassiana, temperature, viability,carrier.

scholarly journals Efek Dolomit Dan Sp-36 Terhadap Bintil Akar, Serapan N Dan Hasil Kacang Tanah (Arachis hypogaea L.) Pada Tanah Kambisol

Agrologia ◽  
2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Charles Silahooy
Keyword(s):  
Root Nodules ◽  
N Uptake ◽  
Phosphate Fertilizer ◽  
Randomized Design ◽  
Arachis Hypogaea L ◽  
Two Factors ◽  
National Production ◽  
Peanut Production ◽  
Completely Randomized Design ◽  
Fertilizer Combination

Maluku in general and Ambon municipality in particular is a region with the lowest peanut production as compared to its national production. Cambisol is one of soil type that has good soil physical properties, but it is low in N and P contents and has an acidic reaction that inhibit the growth of peanut plants.  The research was conducted in a plastic house in Halong Village, Teluk Ambon Baguala Subdistrict, from April to Juli 2012. This research used a Completely Randomized Design with two factors, i.e. factor 1 with three levels of dolomite fertilization, consisting of D0 (without dolomite), D1 (0.3g dolomite per pot), D2 (0.6 g dolomite per pot), and factor 2 with three levels of phosphate fertilization consisting of P0 (without phosphate fertilizer), P1 (0.7 g SP-36 per pot) and P2 (1.4 SP-36 per pot). The results showed that the application of SP-36 increased the number of root nodules and yield of peanut. Response was even better if accompanied by the application of dolomite. Leaf N uptake could be enhanced by application SP-36, but it was independ from the application of dolomite. The highest peanut yield of 41.83 g per pot was achieved with the fertilizer combination of D1P2  (0.3 g dolomite per pot and  1.4 g SP36 per pot)

scholarly journals Physiological potential of Dyckia spp. bromeliad seeds under different temperatures

2021 ◽  
Vol 42 (5) ◽  
pp. 2639-2650
Author(s):  
Verônica Pellizzaro Moresco ◽  
◽  
Mônica Satie Omura ◽  
Jean Carlo Baudraz de Paula ◽  
Felipe Favoretto Furlan ◽  
...  
Keyword(s):  
Growth And Development ◽  
Ornamental Plants ◽  
Dry Weight ◽  
Natural Environments ◽  
The Third ◽  
Tukey Test ◽  
Seed Physiology ◽  
Randomized Design ◽  
Different Temperatures ◽  
Completely Randomized Design

Bromeliads have been gaining ground in the economic scenario due mainly to their use as ornamental plants. Bromeliads of the genus Dyckia exhibit different morphologies, and therefore, have relevance in this market. However, some species are threatened due to the extraction of plants from their natural environments. Many of these species are endemic and native to Brazil and there are few studies on their seed physiology; hence, expansion of research into this topic is essential. An alternative for the preservation of such plants is the production of seedlings using seeds. To this end, the objective of this work was to evaluate the physiological potential of seeds of Dyckia brevifolia, Dyckia beateae, and Dyckia excelsa at different temperatures, and determine the number of days to carry out the tests. A completely randomized design was adopted, with three species of Dyckia, analyzed separately, and four temperatures (20, 25, 30, and 35 °C). The following were evaluated: first germination count, germination, germination speed index, length of seedling, and seedling dry weight. The data were analyzed using the Sisvar software for normality and homogeneity, and when the assumptions were met, they were subjected to analysis of variance using the F test (p < 0.05) and the means were compared using the Tukey test at 5% probability. Seeds of D. excelsa emit the radicle more quickly at 20 and 35 °C; however, temperatures of 25 and 30 °C provide better conditions for seedling growth and development. The greatest vigor of D. brevifolia seeds is observed at temperatures of 30 and 35 °C. Seeds of D. beateae emit the radicle more quickly at 20 °C, and the largest seedlings are observed at 35 °C. The first germination count of D. brevifolia, D. beateae, and D. excelsa should be evaluated on the third day after sowing. The germination evaluations for D. excelsa and D. beateae should be carried out on the ninth day after sowing, and those for D. brevifolia on the sixth day after sowing.

scholarly journals EFFECT OF SUPPLEMENTED SUCROSE AND INJECTED INSULIN UNDER DIFFERENT RECOVERY TIME ON GLUCOSE, FRUCTOSE AND RIBOSE MEAT LAMB

2020 ◽  
Vol 1 (1) ◽  
pp. 60-64
Author(s):  
Elis Dihansih ◽  
Eddie Gurnadi ◽  
C. Hanny Wijaya ◽  
Rudy Priyanto
Keyword(s):  
Body Weight ◽  
Recovery Time ◽  
Sugar Content ◽  
Insulin Injection ◽  
Flavor Quality ◽  
Raw Meat ◽  
The Third ◽  
Randomized Design ◽  
Recovery Times ◽  
Completely Randomized Design

An experiment was conducted in order to study the influence of supplemented sucrose and  injected insulin under  different recovery  times following  transportation on lamb flavor quality. For the purpose the study used fifty four female local lamb (10 to 12 months of  age)  with  weight  ranging  from  14  to  17  kg.  The  experimental lamb  were assigned into a completely randomized design with 2x3x3 factorial arrangement with 3 replications. The first factor was sucrose supplementation with 2 levels (0 and  6 g/kg body weight).  The second factor was insulin injection  after  transportation with 3 levels (0, 0,3 and 0,6 IU). The third factor was the duration of recovery  times with 3 levels (2, 4 and 6 h prior to slaughtering). Parameters measured were glucose, fructose, and ribose. Results showed that   feeding  sugar and  injecting  insulin to  lamb  following  transportation increased glucose, fructose, and  ribose content of raw  meat while recovery  time  was not  affect meat sugar content. Interaction was not occur  between treatment of Sugar, insulin, and recovery  time.

scholarly journals PENGARUH MACAM PUPUK ORGANIK PADAT DAN INTERVAL PEMBERIAN PUPUK ORGANIK CAIR PADA PERTUMBUHAN DAN HASIL CABAI RAWIT

2020 ◽  
Vol 20 (1) ◽  
pp. 36-46
Author(s):  
Daryanti ◽  
Tyas Soemarah K.D ◽  
Muharram Indrawan ◽  
Teguh Supriyadi
Keyword(s):  
Organic Fertilizer ◽  
Growth And Yield ◽  
Soil Types ◽  
Basic Design ◽  
Daily Consumption ◽  
Factorial Method ◽  
Randomized Design ◽  
Completely Randomized Design

Cayenne pepper was a vegetable needed in the daily consumption of people in Indonesia. Efforts to increase the production of cayenne pepper include fertilization. The purpose of this study was to determine the effect of various kinds of organic fertilizer and the interval of liquid organic fertilizer on the growth and yield of cayenne pepper. This study used polybags in plastic houses, from January to May 2019 in Bangsri Village, Karangpandan District, Karanganyar Regency with a height of 450 MASL with Grumosol soil types. This research used a factorial method with the basic design of a Completely Randomized Design (CRD) consisting of 2 treatment factors and is repeated 3 times. The first factor was the type of organic fertilizer (M) consisting of 3 levels, M0 = Soil without organic fertilizer (control), M1 = Soil and Blotong Fertilizer (ratio 1: 1), M2 = Petroganic Soil and Fertilizer (ratio 1: 1 ). The second factor was the interval of adduction liquid organic fertilizer (P) consisting of 3 levels, P0 = Without the adduction of POC (liquid organic fertilizer) (control), P1 = adduction POC every 7 days, P2 = adduction POC every 14 days. The use of organic blotong or Petroganik fertilizer mixed with soil with a ratio of 1: 1 accompanied by the administration of POC Nasa every 14 days produces the highest number and weight of fruit.

scholarly journals Herbicide selectivity in alfalfa crops

2013 ◽  
Vol 31 (4) ◽  
pp. 903-918 ◽  
Author(s):  
N. Hijano ◽  
P.A. Monquero ◽  
W.S. Munhoz ◽  
M.R. Gusmão
Keyword(s):  
Plant Height ◽  
Soil Texture ◽  
Untreated Control ◽  
Clay Soil ◽  
Recommended Dose ◽  
Herbicide Application ◽  
The Third ◽  
Randomized Design ◽  
Chlorimuron Ethyl ◽  
Completely Randomized Design

This study aimed to determine the selectivity of herbicides applied in pre- and post-emergence for alfalfa crops. Three separate experiments were carried out under greenhouse conditions. The first experiment was arranged in a completely randomized design with three replications in a 4 x 11 + 1 factorial scheme , with eleven herbicides (bentazon, chlorimuron-ethyl, fomesafen, fluazifop-p-butyl, saflufenacil, imazethapyr, clethodim, nicosulfuron, imazaquin, haloxyfop-methyl and MSMA), four doses of each herbicide (0.5 D, 0.75 D, 1.0 D and 1.25 D, where D = recommended dose), plus an untreated control. The products were applied to alfalfa plants at the stage of 4 to 5 leaf pairs. In the second experiment, the effect of pre-emergent herbicides on early alfalfa development was observed through a completely randomized design with five replications in a 3 x 4 x 2 factorial scheme, with three herbicides (hexazinone, atrazine + simazine, S-metolachlor), four doses (0.5 D, 0.75 D, 1.0 D and 1.25 D), and two types of soil texture (loamy and clay soil), plus an untreated control. The third experiment evaluated the action of atrazine, 2,550 g ha-1; clomazone - 600 g ha-1; diclosulam - 25 g ha-1; diuron+hexazinone - 936 + 264 g ha-1 and diuron+hexazinone +sulfometuron - 1,386 + 391 + 33.35 g ha-1 on alfalfa sown at different times after herbicide application. The effects of the treatments on alfalfa were evaluated according to visual phytotoxicity symptoms, plant height, and biomass of roots and shoots. Among the herbicides applied at post-emergence, imazethapyr, clethodim, haloxyfop-p-methyl and MSMA were selective for alfalfa, while among those applied at pre-emergence, none were selective, regardless of soil texture. The results of the third experiment showed that the herbicides diclosulam, hexazinone + diuron and atrazine caused less toxicity in alfalfa plants.

scholarly journals Mutasi Induksi Dendrobium sylvanum var. flava Menggunakan Kolkisin secara In Vitro

2018 ◽  
Vol 9 (1) ◽  
pp. 54-62
Author(s):  
Musalamah , ◽  
Ni Made Armini Wiendi ◽  
Sri Rianawati
Keyword(s):  
Analysis Of Variance ◽  
Colchicine Treatment ◽  
Chloroplast Number ◽  
The Third ◽  
Proliferation Medium ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRACTIn Vitro mutation using colchicine on 2 month of self-pollinated protocorm like bodies of Dendrobium sylvanum var. flava was conducted to determine the effects of concentration and immersion duration in colchicine on proliferation of PLBs, and to identify of ploidy variants based on stomatal variable. Research was arranged using factorial completely randomized design with three factors in three replications. The first factor was concentration of colchicine, consisted of five concentrations (0.02; 0.04, 0.06; 0.08; dan 0.1%). The second factor was duration of immersion in the colchicine, consisted of four durations (1; 24; 48; 72 hours). The third factor was proliferation medium consisted of two concentrations of BAP (1; 0.5 mg L-1). Analysis of variance showed the significant effect of colchicine treatment on percentage of survived explants. LD50 in media 1 mg L-1 BAP was obtained at a colchicine concentration of 0.069% with duration immersion of 58.19 hours. On Media 0.5 mg L-1 BAP, LD50 was obtained at colchicine concentration of 0.054% with duration immersion of 47.63 hours. Percentage of solid polyploid mutant of Dendrobium sylvanum var. flava can not be determined on MV2 generation because the stomata leaf showed chimeras based on the chloroplast number in cell guard and stomata size.Keywords: colchicines, Dendrobium sylvanum, mutation, number of chloroplast stomatal density.ABSTRAKMutasi dengan kolkisin pada PLBs hasil selfing Dendrobium sylvanum var. flava umur 2 bulan dilakukan secara In Vitro dengan tujuan mempelajari pengaruh konsentrasi kolkisin, durasi perendaman dalam kolkisin, media proliferasi terhadap pertumbuhan PLBs Dendrobium sylvanum var. flava serta mengidentifikasi variasi ploidi berdasarkan variabel stomata. Penelitian disusun dalam Rancangan Acak Lengkap Faktorial 3 Faktor dengan 3 ulangan. Faktor pertama ialah konsentrasi kolkisin yang terdiri atas 5 taraf (0.02; 0.04, 0.06; 0.08; dan 0.1%). Faktor kedua ialah durasi perendaman yang terdiri atas 4 taraf (1; 24; 48; 72 jam). Faktor ketiga ialah media proliferasi media V&W yang ditambah BAP terdiri atas 2 taraf (1; 0.5 mg L-1). Hasil analisis ragam menunjukkan pengaruh kolkisin yang nyata terhadap variabel persentase hidup. LD50 pada media 1 mg L-1 BAP diperoleh pada konsentrasi 0.069 % dengan durasi perendaman 58.19 jam. Pada media 0.5 mg L-1 BAP, LD50 diperoleh pada konsentrasi 0.054 % dengan durasi perendaman 47.63 jam. Persentase mutan poliploid pada MV2 Dendrobium sylvanum var. flava ini belum dapat ditentukan karena stomata daunnya masih kimera berdasarkan karakter jumlah kloroplas sel penjaga dan ukuran stomata.Kata kunci: Dendrobium sylvanum, jumlah kloroplas, kerapatan stomata, kolkisin, mutasi.

scholarly journals Efektivitas Antioksidan Likopen Pada Buah Tomat (Lycopercisum esculentum) Terhadap Normalitas dan Abnormalitas Spermatozoa Sapi Bali dengan Metode Swim up.

bionature ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Syuhriatin Syuhriatin
Keyword(s):  
Storage Time ◽  
Lower Layer ◽  
Tomato Fruit ◽  
Treatment Combination ◽  
The Third ◽  
Randomized Design ◽  
Negative Effect ◽  
Completely Randomized Design ◽  
The Difference ◽  
Artificial Vagina

Abstract. This research is a type of experimental research carried out in a laboratory with the aim of knowing a symptom or effect that arises as a result of certain treatments. The research was conducted at the Immunobiology Laboratory, Faculty of Mathematics and Natural Sciences, Mataram University. The material used in the study was 1 male Bali cow, about 3.5 years old with a cow weight of 460 kg, which the semen was collected using an artificial vagina. The experimental design used in this study was a completely randomized design which was arranged in a factorial design consisting of 3 (three) factors. The first factor is the difference in layer (L) which consists of 2 (two) levels, namely the Upper Layer (LA) and the Lower Layer (LB). The second factor is the concentration of tomato lycopene (P) consisting of 5 (five) levels, namely: 0% (P0), 5% (P1), 10% (P2), 15% (P3) and 20% (P4). The third factor is storage time (H) which consists of 6 (six factors), namely 1 day (H1), 2 days (H2), 3 days (H3), 4 days (H4), 5 days (H5) and 6 days. (H6). The three factors combined to produce 60 treatment combinations, each treatment combination was repeated 6 (six) times so that there were 360 experimental units. The results of the analysis of variance showed that the addition of the tomato fruit lycopene antioxidant and the separation by the swim up method had no significant effect (P> 0.05) on the normality and abnormality of spermatozoa. The addition of tomato fruit lycopene antioxidants and the separation using the swim up method did not have an effect on increasing spermatozoa abnormalities, but did not have a negative effect on the spermatozoa of bali cattle.Keywords: antioxidant, tomato fruit, spermatozoa, bali cow

PENGARUH BERBAGAI ZAT PENGATUR TUMBUH (ZPT) TERHADAP PERTUMBUHAN STEK BATANG PULAI RAWA (Alstonia spatulata)

2021 ◽  
Vol 4 (2) ◽  
pp. 198
Author(s):  
Winda Ningsih Pardede ◽  
Gusti Muhammad Hatta ◽  
Damaris Payung
Keyword(s):  
Root Growth ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Growth Response ◽  
Stem Cuttings ◽  
The Third ◽  
Randomized Design ◽  
Plant Stem ◽  
Completely Randomized Design ◽  
Bean Sprouts

Some of the goals that exist in this research are to analyze how the effect of giving Rootone F, giving Rootmost and giving Bean Sprouts Extract to the growth response of stem cuttings in Pulai Rawa (Alstonia spatulata. Growth Regulatory Substances used in this study there are 3 types of treatments such as giving Rootone F as the first treatment, the second treatment is by giving rootmost and by giving Bean Sprouts Extract as the third treatment and in this study there is control as one of the comparison between the differences between Stem cuttings that use Growth Regulatory Substances with no provision of ZPT. In November 2019 until January 2020 this study took place in the shade house of the forestry faculty at Lambung Mangkurat University.  RAL (Completely Randomized Design) The method used in this study is to use with a set of 4 treatments that were repeated 20 times in each treatment, then there were 80 experimental units in this study.  The results obtained in this study showed the effect of various growth regulators which differed to the response of the growth of Pulai Rawa plant stem cuttings.  The provision of rootone F did not affect the growth response while the administration of Growth Regulator Substance Rootmost gave an effect on the response of root growth of Pulai Rawa cuttings (Alstonia spatulata) and by giving Bean Sprout Extract which had an influence on the growth in number of cuttings.Keywords : Growth regulators; Stem cuttings; Pulai Rawa

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