ROOT INDUCTION OF PATCHOULI PLANT STEM CUTTINGS USING IBA GROWTH REGULATOR ON SEVERAL PLANT MEDIA

Patchouli (Pogostemon cablin Benth.) plant is one of the essential oil producing plants, that is useful in the chemical industry as a raw material for fragrance products and in the pharmaceutical field. The success rate of patchouli early growth, is largely determined by the cultivation techniques used, including the use of growth regulators, and growing media. This study aims to determine the effect of IBA growth regulators and the composition of plant media on the growth of patchouli plants. This research is carried out in the Greenhouse of the Forestry Study Program of the University of Mataram, using a Factorial Completely Randomized Design (RALF), the first factor is IBA growth regulator with 3 levels, namely 0, 20, 25 ppm and the second factor is composition of plant media with 3 levels, namely forest soil media, soil media:sand:compost (1:1:1), and soil media:sand:compost (2:1:3). The results show that the effect of IBA significantly affect the success percentage of cuttings and the number of roots of patchouli plants. The effect of plant media composition and the interaction between treatment of IBA with plant media composition did not have a significant effect on the growth parameters of patchouli plants.

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PENGARUH BAHAN ASAL STEK DAN MEDIA TANAM TERHADAP PERTUMBUHAN NILAM (Pogostemon cablin Benth)

Jurnal Silva Samalas ◽

10.33394/jss.v3i1.3675 ◽

2020 ◽

Vol 3 (1) ◽

pp. 1

Author(s):

River Wedilen Bona Simatupang ◽

Irwan Mahakam Lesmono Aji ◽

Dwi Sukma Rini

Keyword(s):

Growth Parameters ◽

Stem Cutting ◽

Growth Effect ◽

Stem Cuttings ◽

Study Program ◽

Randomized Design ◽

Pogostemon Cablin ◽

Soil Media ◽

Four Levels ◽

Cultivation Techniques

Patchouli plant (Pogostemon cablin Benth.) is one of the essential oil producing plants that is useful in the fields of pharmaceutical, cosmetics and perfume as a mixture or fixation (binding of fragrances). The success rate of patchouli early growth, is largely determined by the cultivation techniques used, one of which includes vegetative cultivation of various selected stem cutting, and planting media. This study aims to determine the effect of material from cuttings and planting media on the growth of patchouli plants. The research was conducted at the Greenhouse of the University of Mataram Forestry Study Program, using a Factorial Completely Randomized Design (FCRD). The first factor is various stem cutting with three levels, namely tip stem cuttings, middle stem cuttings and base stem cuttings and the second factor is planting media with four levels, namely forest soil media, soil:cocopeat (1:1), soil:cocopeat (1:2) and soil:cocopeat (2:1). The results showed that the effect of material from stem significantly affected the percentage of life, number of shoots, number of leaves, shoot length. For the planting media, it had a significant effect on the length of the shoot, shoot diameter, leaf area, dry trimming weight. The interaction effect of various stem cutting and plan media did not have a significant effect on the growth parameters of patchouli. The treatment of material from cuttings in patchouli plants had the best growth effect on the treatment of S2 (middle stem cuttings) and the treatment of planting media had the best growth effect on the M0 (soil media) treatment.

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PENGARUH BERBAGAI ZAT PENGATUR TUMBUH (ZPT) TERHADAP PERTUMBUHAN STEK BATANG PULAI RAWA (Alstonia spatulata)

Jurnal Sylva Scienteae ◽

10.20527/jss.v4i2.3329 ◽

2021 ◽

Vol 4 (2) ◽

pp. 198

Author(s):

Winda Ningsih Pardede ◽

Gusti Muhammad Hatta ◽

Damaris Payung

Keyword(s):

Root Growth ◽

Growth Regulators ◽

Growth Regulator ◽

Growth Response ◽

Stem Cuttings ◽

The Third ◽

Randomized Design ◽

Plant Stem ◽

Completely Randomized Design ◽

Bean Sprouts

Some of the goals that exist in this research are to analyze how the effect of giving Rootone F, giving Rootmost and giving Bean Sprouts Extract to the growth response of stem cuttings in Pulai Rawa (Alstonia spatulata. Growth Regulatory Substances used in this study there are 3 types of treatments such as giving Rootone F as the first treatment, the second treatment is by giving rootmost and by giving Bean Sprouts Extract as the third treatment and in this study there is control as one of the comparison between the differences between Stem cuttings that use Growth Regulatory Substances with no provision of ZPT. In November 2019 until January 2020 this study took place in the shade house of the forestry faculty at Lambung Mangkurat University. RAL (Completely Randomized Design) The method used in this study is to use with a set of 4 treatments that were repeated 20 times in each treatment, then there were 80 experimental units in this study. The results obtained in this study showed the effect of various growth regulators which differed to the response of the growth of Pulai Rawa plant stem cuttings. The provision of rootone F did not affect the growth response while the administration of Growth Regulator Substance Rootmost gave an effect on the response of root growth of Pulai Rawa cuttings (Alstonia spatulata) and by giving Bean Sprout Extract which had an influence on the growth in number of cuttings.Keywords : Growth regulators; Stem cuttings; Pulai Rawa

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PERTUMBUHAN STEK HORIZONTAL BATANG JARAK PAGAR (Jatropha curcas L.) YANG DIINTRODUKSI DENGAN PSEUDOMONAD FLUORESEN

EKSAKTA Berkala Ilmiah Bidang MIPA ◽

10.24036/eksakta/vol19-iss1/129 ◽

2018 ◽

Vol 19 (1) ◽

pp. 68-75

Author(s):

Linda Advinda

Keyword(s):

Jatropha Curcas ◽

Indole Acetic Acid ◽

Shoot Length ◽

Raw Material ◽

Fluorescent Pseudomonad ◽

Stem Cuttings ◽

Jatropha Curcas L ◽

Randomized Design ◽

Shoot Number ◽

Number Of Shoots

Jatropha curcas L. is one of the plants that can be developed as raw material of biodiesel. One of the efforts to increase the production of J. curcas is the propagation of stem cuttings horizontally. Efforts to stimulate root growth can be accomplished by adding Plant Growth Regulators (PGRs). PGRs can be obtained naturally or synthetically. Indole acetic acid (IAA) is one of PGRs, which is not only produced by plants but can also be produced by rhizobacteria. One such rhizobacteria is fluorescent pseudomonad. The objectives of the study were to know the response of growth of horizontal stem cuttings after being introduced with fluorescent pseudomonad. This research is an experimental research. Complete Randomized Design with 6 treatments and 4 replications. The treatments were soaking of J. curcas stem cuttings with fluorescent pseudomonad isolate suspension PfPj3 suspension concentration of 0% (without suspension), 5%, 10%, 15%, 20%, and 25%. Observation of the research is the beginning of the shoots, the number of shoots, and the length of the J. curcas stem shoot. The data obtained were analyzed using Anova and DNMRT significant level of 5%. The results showed that horizontal stem cuttings of J. curcas with fluorescent pseudomonad suspension influenced early shoot, shoot number, and shoot length. A 10% fluorescent pseudomonad concentration can cause shoots to appear early, while a concentration of 20% causes the largest number of shoots.

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Growth Response of Melati Plant Plant (Jasminum Sambac L.) Against Types of Media Plants and Types of Growing Agents

Budapest International Research in Exact Sciences (BirEx) Journal ◽

10.33258/birex.v2i2.893 ◽

2020 ◽

Vol 2 (2) ◽

pp. 188-200

Author(s):

Yuliatul Muslimah ◽

Sumeinika Fitria Lizmah ◽

Nur Fayanti

Keyword(s):

Growth Regulators ◽

Growth Regulator ◽

Root Length ◽

Alluvial Soil ◽

Randomized Design ◽

Number Of Leaves ◽

Media Types ◽

The Difference ◽

Jasminum Sambac ◽

Number Of Shoots

Propagation of jasmine plants by cuttings often experience obstacles, especially to grow roots. Proper planting media and application of growth regulators (PGR) have an important role in the growth and development of plant cuttings roots. The purpose of this study was to determine the effect of planting media types and PGR on the growth of jasmine cuttings. This research was conducted in the experimental garden of the Faculty of Agriculture, Teuku Umar University, in March - May 2019. This study used a completely randomized design (CRD) with a factorial pattern of 3x3 with three replications. The treatment factors studied were (1) the type of planting media consisted of 3 levels namely alluvial soil, sand and husk charcoal, and (2) the type of growth regulator (ZPT) consisted of 3 levels namely control (without zpt), chemical (growtone) and organic (shallot extract). F test results on the analysis of variance showed that the planting medium had a very significant effect on the number of shoots 35 and 45 HST, number of leaves 15, 35 and 45 HST number of roots, root length, percentage of live cuttings. But the planting media had no significant effect on the number of shoots of 15 DAP. Growth regulator substances have a very significant effect on the number of shoots 35 HST, number of leaves 15, 35 and 45 HST, number of roots and root length. Significantly affect the number of shoots 45 HST and percentage of live cuttings, but no significant effect on the number of shoots 15 HST. This shows that the difference in growth of jasmine plant cuttings due to different planting media does not depend on growth regulators, and vice versa.

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Multiplikasi Tunas dan Induksi Perakaran Pada Perbanyakan Rhododendron radians J.J.Sm (Ericaceae) Secara In Vitro [Shoot Multiplication and Root Induction on In Vitro Propagation of Rhododendron radians J.J.Sm (Ericaceae)]

Jurnal Hortikultura ◽

10.21082/jhort.v28n1.2018.p51-58 ◽

2019 ◽

Vol 28 (1) ◽

pp. 51

Author(s):

Tri Warseno ◽

Dyan Meiningsasi Siswoyo Putri

Keyword(s):

Growth Regulators ◽

Shoot Proliferation ◽

Shoot Multiplication ◽

Root Induction ◽

Plant Materials ◽

Shoot Height ◽

Randomized Design ◽

Starting Point ◽

Four Levels

Rhododendron radians merupakan tanaman hias yang hanya ditemukan di Sulawesi Tengah dan Utara. Perbanyakan secara massal diperlukan untuk komersialisasi tanaman tersebut. Penelitian bertujuan mendapatkan teknik mikropropagasi secara in vitro yang tepat untuk R. radians melalui percobaan pemberian zat pengatur tumbuh (ZPT) pada media kultur. Penelitian terdiri atas dua tahap percobaan. Percobaan 1 perlakuan kombinasi ZPT untuk proliferasi tunas disusun menggunakan rancangan acak lengkap dengan satu faktor, yaitu media (delapan kombinasi media) dengan menggunakan dua konsentrasi IAA (0,1 mg/l dan 1,0 mg/l) dan empat konsentrasi 2iP (0, 6, 7, dan 8 mg/l). Percobaan 2 adalah perlakuan IBA untuk pertumbuhan tunas dan pengakaran menggunakan lima variasi media (M1 = media WPM (kontrol), M2 = media WPM + 0,25 mg/l IBA, M3 = media WPM + 0,5 mg/l IBA, M4 = media WPM + 1 mg/l IBA, M5 = media WPM + 5 mg/l IBA). Hasil penelitian menunjukan bahwa media WPM dengan penambahan 1,0 mg/l IAA dan 7,0 mg/l 2iP merupakan media terbaik untuk induksi tunas pada R. radians, dengan jumlah tunas rata-rata 15,80 + 3,45 cm dan tinggi tunas rata-rata 2,36 + 0,25 cm, sedangkan media terbaik untuk induksi akar adalah media M4 (WPM + 1,0 mg/l IBA), dengan persentase eksplan berakar 63,33% dan panjang akar rata-rata 4,7 mm. Hasil penelitian diharapkan dapat digunakan sebagai acuan awal untuk melakukan perbanyakan secara massal R. radians dan diharapkan penelitian lebih lanjut dapat dilakukan sampai dengan tahap aklimatisasi.KeywordsAuksin; Media; Mikropropagasi; Rhododendron radiansAbstractRhododendron radians is an ornamental plant that found only in Sulawesi (Central and North). Commercialization of this species requires mass propagated plant materials. This study aimed to determine the proper technique for R. radians micropropagation in vitro. This study used two stages of treatment i.e (1) treatment of IAA on shoot proliferation R. radians arranged in completely randomized design with one factor (eight medium combination), the concentration of plant growth regulators IAA which consisted of two levels: 0.1 mg/l, and 1 mg/l and concentration of growth regulators 2iP consisting of four levels (0,6, 7, and 8 mg / l); (2) IBA treatment on shoot growth and rooting using five variations of the medium (M1 = WPM (control), M2 = WPM + 0.25 mg/l IBA, M3 = WPM + 0.5 mg/l IBA, M4 = WPM + 1 mg/l IBA; M5 = WPM + 5 mg/l IBA). The results showed that the WPM added with 1 mg/l IAA and 7 mg/l 2iP was the best medium for shoot induction initiated from seed culture, with the average number of shoots 15. 80 ± 3.45 cm and an average shoot height of 2.36 ± 0.25 cm. While the best medium for root induction was M4 (WPM + 1 mg / l IBA), with a percentage of 63.33% rooted explants and the average root length of 4.7 mm. The results of this study can be used as a starting point to conduct mass propagation R. radians.

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Pengaruh Beberapa Dosis Pupuk Dan Beberapa Media Tumbuh Terhadap Pertumbuhan Bibit Jabon (Anthocephalus Cadamba)

Jurnal Sylva Lestari ◽

10.23960/jsl1231-40 ◽

2014 ◽

Vol 2 (1) ◽

pp. 31

Author(s):

Sondri Kurniawan ◽

Afif Bintoro ◽

Melya Riniarti

Keyword(s):

Rice Husk ◽

Root Length ◽

Growth Parameters ◽

Dry Weight ◽

Media Composition ◽

Soil Medium ◽

Randomized Design ◽

Number Of Leaves ◽

Total Dry Weight ◽

Anthocephalus Cadamba

Jabon wood (Anthocephalus cadamba) is a fast growing species.Currently, jabon wood is play an important rile for timber industry, included plywood, lamina, and the other timber industry.To improve the quality of wood jabon, necessary for the addition of fertilizer and improved media.Application of multiple doses of fertilizers with several media compositions was expected could increase the growth of jabon wood seedling.This observation aimed to know the effect and interaction of media composition and fertilizer on the growth of jabon wood seedling.The observation was conducted in greenhouse of Agriculture Faculty, Lampung University for 4 months.The observation was compiled factorially (3x4) in a completely randomized design (CRD) with 3 replications. The first factor was the composition of the soil medium consisting of red-yellow podzolic (100%); red-yellow podzolic + sand (1:1); red-yellow podzolic + rice husk (1:1), while the second factor was the dose of fertilizer consists of 0 g, 0.5 g , 1 g, and 1.5 g. The observed variables are added height, diameter, number of leaves, root length, and total dry weight.The results showed that media composition significantly affected, diameter, number of leaves and total dry weight.Fertilizer did not significantly affected height increment, diameter, number of leaves, root length, and total dry weight, there was no interaction between several media compositions with fertilizer. The best treatment to all growth parameters was red-yellow podzolic soil + sand (1:1) with all fertilizer doses. Keywords: fertilizer, jabon wood, podzolic,red-yellow, rice husk

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Induction of Barangan Banana shoot (Musa acuminata L.) from North Nias through Tissue Culture by giving 2,4-D and Kinetin

JURNAL BIOSAINS ◽

10.24114/jbio.v1i2.2780 ◽

2016 ◽

Vol 1 (2) ◽

pp. 1

Author(s):

Destarius Zebua ◽

Suci Rahayu ◽

Saleha Hanum

Keyword(s):

Tissue Culture ◽

Growth Regulators ◽

Growth Regulator ◽

Musa Acuminata ◽

Basal Part ◽

Banana Weevil ◽

Shoot Induction ◽

Randomized Design ◽

Completely Randomized Design ◽

Basal Position

The research on induction of banana barangan shoot (Musa acuminata L.) from North Nias through tissue culture by giving 2,4-D and kinetin was conducted in the Laboratory of Tissue Culture University of North Sumatera from May until October 2014. The main objective of this research was to know the explants of banana weevil in apical and basal position with growth regulator 2,4-D with kinetin which are able to induce shoots from callus initials. On this research, the treatment tested for induction of shoots was growth regulators 2,4-D in the concentration 0 mg/L, 1 mg/L, 1,5 mg/L, 2 mg/L and 2,5 mg/L with growth regulators kinetin in the concentration 0 mg/L, 5 mg/L, 6 mg/L, 7 mg/L and 8 mg/L. The research was designed using Completely Randomized Design (CRD) two factorial with repetition of experiment. The results of research showed that the interaction between concentration of 2,5 mg/L 2,4-D with 5 mg/L kinetin was fastest (79 days) forming the shoot derived from explant basal part. The concentration of 2,5 mg/L 2,4-D with 5 mg/L kinetin produced the average number of shoots formed for 3.00 and average number of highest shoots for 1.50 cm. Keywords: Musa acuminata, weevil, initiation, callus, shoot, induction, barangan banana, 2,4-D, kinetin.

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Penggunaan Air Kelapa Untuk Setek Batang Jati (Tectona Grandis)

Jurnal Sylva Lestari ◽

10.23960/jsl1461-68 ◽

2016 ◽

Vol 4 (1) ◽

pp. 61

Author(s):

Rega Renvillia ◽

Afif Bintoro ◽

Melya Riniarti

Keyword(s):

Growth Regulator ◽

Water Immersion ◽

Tectona Grandis ◽

Stem Cutting ◽

Coconut Water ◽

Stem Cuttings ◽

Randomized Design ◽

Root Cutting ◽

Water Cutting ◽

Completely Randomized Design

Teak plant wasa treewhich timber has durability classI and strong class I. It hasmany benefits, from roots to leaves. Variousbenefits of teak causing to do cultivation, one of propagation activities that often carried out was vegetative. Vegetative propagation of plants can be done bystem cutting, root cutting and shoot cutting. Using of growth regulator with coconut water immersion is expected to spur the growth rootofteak stem cutting. This study aimed to determine the effect of using coconut water to increase the success of teak stem cuttinggrowth, andto determine the best dose of using coconut water forteak stem cutting growth. This treatment used Completely Randomized Design(CRD). This research consisted of five treatments that usedcoconut water treatment 0%, 25%, 50%, 75%, and 100%. Each treatmentconsisted of five teak stem cuttings with repetition as much as 5 times. Amount ofmaterial plants required 5 x 5 x 5 = 125 teak stem cuttings. The resultshowed by using coconut wateras a growth regulator could increasethe growth of teak (Tectona grandis) stem cuttingin 50%--100% concentration and the best dose of coconut water asa growth regulator was 100%. Keywords : coconut water, cutting, dose, growth regulator, teak

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The Effect of Plant Growth Regulator on Duku (Lansium domesticum Corr.) Flower for Fruit Formation

Ilmu Pertanian (Agricultural Science) ◽

10.22146/ipas.28293 ◽

2018 ◽

Vol 2 (3) ◽

pp. 100

Author(s):

Susilawati Susilawati ◽

Lidwina Ninik S. ◽

Mery Hasmeda ◽

Irmawati Irmawati

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Growth Regulator ◽

Plant Growth Regulator ◽

Fruit Weight ◽

Randomized Design ◽

Fruit Maturity ◽

Lansium Domesticum ◽

Fruit Formation

Plant growth regulators have a significant impact on increasing both quality and quantity of plant yield for either mixed or separated application. The research was aimed to identify the effect of spraying plant growth regulators on duku flowers for fruit formation. The research was conducted from December 2014 to May 2015 in Sijacarana Local Technical Implementation Unit (UPTD) of Propagation, South Sumatra Province in Ogan Komering Ulu (OKU) Timur Regency. Duku tree used was 15 years old with the height around 10 meter. Complete Randomized Design was used with 3 replications where duku trees as the replicates. The spraying consisted of 3 single plant growth regulator treatments and 6 mixed treatments which were P1 (BAP 300 mg.L-1), P2 (NAA 300 mg.L-1), P3 (GA3 300 mg.L-1), P4 (BAP 100 mg. L-1 + GA3 200 mg.L-1), P5 (BAP 200 mg.L-1 + GA3 100 mg.L-1), P6 (BAP 150 mg.L-1 + GA3 150 mg.L-1), P7 (NAA 100 mg.L-1 + GA3 200 mg.L-1), P8 (NAA 200 mg.L-1 + GA3 100 mg.L-1), P9 (NAA 150 mg.L-1 + GA3 150 mg.L-1). Spraying was applied twice with a half dosage for each application. First application was performed about one month after flowering, and the second was one and a half month after flowering. Data were analyzed using analysis of variance and least significance difference at α 5%. The measurement of parameters were carried after harvest or 3 months after treatment. Results showed that plant growth regulator positively affected duku fruit formation. GA3 treatment then was considered as the best treatment as it had a unison in fruit maturity, the highest number of fruit per bunch, weight per fruit and total fruit weight per bunch, and had the lowest number of green fruit and seed per fruit.

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IN VITRO CALLUS INDUCTION OF ‘SHIRAZ’ GRAPE (Vitis vinifera L.) USING DIFFERENT MEDIUM AND GROWTH REGULATOR COMBINATION

International Journal of Biosciences and Biotechnology ◽

10.24843/ijbb.2018.v06.i01.p04 ◽

2018 ◽

Vol 6 (1) ◽

pp. 25

Author(s):

Ni Nyoman Nila Arieswari ◽

Ida Ayu Astarini ◽

Ni Putu Adriani Astiti ◽

Jeremy Pramana

Keyword(s):

Growth Regulator ◽

Callus Induction ◽

Basal Medium ◽

Raw Material ◽

Vitis Vinifera L ◽

Study Program ◽

Wine Production ◽

Propagation Technique ◽

Planting Materials

One of grape cultivars widely used as raw material for wine production is ‘Shiraz’ cultivar. Propagation of this cultivar is necessary for the provision of grape as a wine making material in Bali. In vitro culture is an alternative propagation technique than can be employed to produce planting materials in a shorter time. This research aims to determine the most suitable medium and growth regulator combination in inducing grape cv. ‘Shiraz’ callus in vitro. The study was conducted from November 2017 until February 2018 at Plant Tissue Culture Laboratory, Biology Study Program, Udayana University. The explants used were young stem of grape cv. ‘Shiraz’ and the experiment was conducted using factorial design with two factors. The first factor was basal medium used (MS and WPM) and the second factor was IBA concentration (0; 0.5 and 1 mgL-1) and BAP (0, 1 and 2 mgL-1). The results showed that the highest percentage of callus induction (60%) was obtained on WPM medium without growth regulator combination (control). However, the fastest time of callus appear was on MS medium + 2 mgL-1 BAP without IBA, which was 17 days after planting. The texture and color of callus resulted on this research were friable with white, greenish white, greenish yellow and green in color.

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