scholarly journals Toleransi logam berat timbal (Pb) pada bakteri indigenous dari air laut Pelabuhan Paotere, Makassar (Heavy metal lead [Pb] tolerance of indigenous bacteria from Seawater in Paotere Port, Makassar)

2020 ◽  
Vol 8 (1) ◽  
pp. 8
Author(s):  
Fahruddin Fahruddin ◽  
Slamet Santosa ◽  
Sareda .
Keyword(s):  
Heavy Metals ◽  
Nutrient Broth ◽  
Nutrient Agar ◽  
Resistant Bacteria ◽  
Bacterial Isolation ◽  
Sediment Samples ◽  
Marine Life ◽  
Agar Media ◽  
Seawater Samples ◽  
Polluted Waters

Marine water which has been polluted by heavy metals such as lead (Pb) from port activities can affect marine life, however several microorganisms are able to adapt in polluted waters due to their resistant capabilities. The research aimed to obtain isolates bacterial which are resistant to Pb and to test their resistant ability. Bacterial isolation was obtained from sediment and seawater taken in Paotere Port, Makassar. The isolation of the resistant bacteria was done using nutrient agar media which contained 10 ppm of Pb. The growth of the bacteria was initially observed macroscopically and microscopically to determine type of the colony. Resistant test for each type of isolate was done by inoculating them on nutrient broth media which contained different concentration of Pb: 5 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, and a control. The test resulted in eight different isolates, five isolates from sediment samples and three from seawater samples. The results showed that each isolate has different degree of resistant toward Pb. Isolate IA1 has the higher resistant ability, and it was followed by isolate IS5.---Perairan laut tercemar logam berat timbal (Pb), yang bersumber dari aktifitas pelabuhan, dapat mengganggu kehidupan biota laut, namun sejumlah mikrorganisme mampu beradaptasi karena memiliki kemampuan resistan.  Penelitian ini bertujuan untuk memperoleh isolat bakteri yang resistan terhadap Pb dan menguji kemampuan resistansinya. Isolasi bakteri diperoleh dari sampel sedimen dan air laut dari Pelabuhan Paotere, Makassar.Untuk isolasi bakteri resistan menggunakan media nutrient agar yang megandung 10 ppm Pb. Bakteri yang tumbuh diamati secara makropskopis dan mikroskopis sebagai pendekatan awal untuk mengetahui jenis isolat. Uji resistansi dilakukan untuk setiap jenis isolat, yang diinokulasikan pada media nutrient broth pada konsentrasi Pb sebesar 5 ppm, 10 ppm, 15 ppm. 20 ppm, 25 ppm dan 0 ppm sebagai kontrol.  Hasilnya, diperoleh delapan jenis isolat yang berbeda, yaitu lima jenis isolat dari sampel sedimen dan tiga dari sampel air laut. Dari hasil uji memperlihatkan, bahwa setiap isolat memiliki kemampuan resistansi berbeda-beda terhadap Pb. Isolat IA1 kemampuan resistansinya lebih tinggi, kemudian isolat IS5.

scholarly journals Heavy metal (As, Cd, Cr, Hg, Pb) analysis and identification of heavy metal resistant bacteria in sediments from Manado Bay

2021 ◽  
Vol 926 (1) ◽  
pp. 012096
Author(s):  
B J Kepel ◽  
W Bodhi ◽  
Fatimawali ◽  
T E Tallei
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Average Concentration ◽  
Resistant Bacteria ◽  
Icp Oes ◽  
Sediment Samples ◽  
Heavy Metal Concentrations ◽  
The World ◽  
Heavy Metal Resistant ◽  
16Srrna Gene

Abstract Environmental pollution from heavy metals is becoming a growing concern due to the adverse effects it is causing throughout the world. This study aims to analyze heavy metal concentrations and identify heavy metal resistant bacteria in the bay of Manado. Sediment samples were collected from five bays in Manado. The concentrations of heavy metals As, Cd, Cr, Hg and Pb were analyzed using ICP-OES, and Hg using CV-AFS. Bacteria from the sediment were grown in nutrient broth media containing heavy metals As, Cd, Cr, Hg and Pb respectively. Microbiology and 16SrRNA gene analysis were used to identify the bacteria that grown on media containing varying concentrations of heavy metals. The results showed that the sediments from the five bays in Manado contained heavy metals with an average concentration of As <1mg/kg, Cd 1.8mg/kg), Cr 6.2mg/kg, Hg <0.07mg/kg). and Pb 11.2mg/kg. The results of microbiological and molecular analysis showed that 5 species of heavy metal resistant bacteria were Pseudomonas aeruginosa, Bacillus cereus, Staphylococcus arlettae, Acinobacter sp., and Brevibacterium sp. The five bacteria found to be resistant to heavy metals can be used to detoxify As, Cd, Cr, Hg, and Pb.

scholarly journals Heavy metals concentration in sediment of Makassar Strait

2021 ◽  
Vol 925 (1) ◽  
pp. 012019
Author(s):  
Edward ◽  
Helfinalis ◽  
F Budiyanto ◽  
Y Witasari
Keyword(s):  
Heavy Metals ◽  
Atomic Absorption ◽  
Human Activities ◽  
Atomic Absorption Spectrophotometer ◽  
Sediment Samples ◽  
Marine Life

Abstract The concentration of heavy metals (HMs) camium (Cd), cuprum (Cu), chromium (Cr), nickel (Ni), lead (Pb), and zinc (Zn) in sediments of Makassar Strait was investigated. We collected sediment samples from 28 research stations. Atomic Absorption Spectrophotometer (AAS) was used to measure HMs in all samples. This research aims to know the levels of HMs’ contamination and pollution in sediments. The analysis revealed that the concentration of all HMs is still low and inlined with the sediment standard guidelines, exception of Cu and Ni. In regards to the value of I_geo and PLI, sediment in the Makassar Strait is still normal for marine life, and sediment is included in unpolluted categories (PLI<1). The sources of HMs in this strait come from human activities in the land of Kalimantan and Sulawesi Island.

Ekplorasi dan Karakterisasi Biokimia Bakteri Resisten Timbal (Pb) dari Sungai Tallo Makassar

2020 ◽  
Vol 5 (3) ◽  
Author(s):  
Fahruddin Fahruddin ◽  
Nur Haedar ◽  
Slamet Santosa ◽  
Sri Wahyuni
Keyword(s):  
Heavy Metal ◽  
Water Samples ◽  
Bacterial Isolate ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Sediment Samples ◽  
Agar Media ◽  
Biological Methods ◽  
Selection Of

Industrial progress and waste of urban domestic activities have an impact on the Pb heavy metal pollution on the environment, this will have an impact on human health. Pb metal can be overcome with biological methods by utilizing bacteria in reducing Pb metal. The study aimed to obtain the species  of resistant bacterial isolates of Pb metal and determine its characteristics. Sediment and water samples were obtained from the Tallo River. Isolation and selection of Pb metal resistant bacteria were carried out on nutrient agar media is 10 ppm added of PbAgNO3, afterwards the colonies that grew and differed were characterized by morphological morphology and several biochemical tests. The characterization results obtained 8 isolates of Pb metal resistant bacteria consisted of 8 isolates from sediment samples and 3 isolates from water samples. The characteristics of each bacterial isolate on cell morphology are the same and biochemical tests show different results.

scholarly journals Distribusi Logam Berat Dalam Air Laut Dan Sedimen Di Perairan Cimanuk, Jawa Barat, Indonesia

2020 ◽  
Vol 5 (1) ◽  
pp. 19 ◽  
Author(s):  
Harmesa Harmesa ◽  
Lestari Lestari ◽  
Fitri Budiyanto
Keyword(s):  
Heavy Metals ◽  
Spatial Distribution ◽  
Anthropogenic Activities ◽  
Economic Activities ◽  
Distribution Models ◽  
Sediment Samples ◽  
The North ◽  
Seawater Samples ◽  
High Level

<p><strong>Distribution of Heavy Metals in Seawater and Sediments in Cimanuk Estuary, West Java, Indonesia.</strong><strong> </strong>Increasing economic activities in the north coastal of Java have the potential to contribute to anthropogenic contaminants that threaten the water quality of Cimanuk coastal and estuary in Indramayu. Heavy metal which is one of the wastes from these activities has not been studied in detail. The aim of this study was to determine the distribution of Cu, Pb, Cd, Zn, and Ni in seawaters and sediments of the Cimanuk Estuary. Seawater and sediment samples were collected at 18 stations in May 2017. Seawater samples were extracted using the back extraction method while sediment samples were extracted using acids according to USEPA 3050B. Measurement of heavy metals from seawater extracts or sediments was carried out using Flame Absorption Spectrophotometry according to the USEPA 3050B method. The spatial distribution of heavy metals in seawater and sediments is modeled using ArcGIS® version 10.6.1. The results showed that ranges of metals in seawater : 0,0004 – 0,0038 mg/L (Cu), &lt;0,0001 – 0,0044 mg/L (Pb), 0,0002 – 0,0003 mg/L (Cd), 0,0005 – 0,0119 mg/L (Zn), and 0,0020 – 0,0052 mg/L (Ni). While the metal content in sediments are 12,36 – 54,08 mg/kg (Cu), 6,43 – 15,72 mg/kg (Pb), 0,07 – 0,37 mg/kg (Cd), 64,53 – 85,16 mg/kg (Zn), and 19,66 – 62,85 mg/kg (Ni). Spatial distribution models show that heavy metals in seawater and sediments show identical patterns. High level of metals are generally detected at the stations located closed to the mainland, indicating that heavy metals are enrichment from terrestrial anthropogenic activities.</p>

scholarly journals Resistance of bacteria isolated from leachate to heavy metals and the removal of Hg by Pseudomonas aeruginosa strain FZ-2 at different salinity levels in a batch biosorption system

2021 ◽  
Vol 31 (1) ◽  
Author(s):  
Muhammad Fauzul Imron ◽  
Setyo Budi Kurniawan ◽  
Siti Rozaimah Sheikh Abdullah
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Pseudomonas Aeruginosa ◽  
Fresh Water ◽  
Landfill Leachate ◽  
Saline Water ◽  
Resistant Bacteria ◽  
Sanitary Landfill ◽  
Salinity Levels ◽  
Sanitary Landfill Leachate

AbstractLeachate is produced from sanitary landfills containing various pollutants, including heavy metals. This study aimed to determine the resistance of bacteria isolated from non-active sanitary landfill leachate to various heavy metals and the effect of salinity levels on the removal of Hg by the isolated bacterium. Four dominant bacteria from approximately 33 × 1017 colony-forming units per mL identified as Vibrio damsela, Pseudomonas aeruginosa, Pseudomonas stutzeri, and Pseudomonas fluorescens were isolated from non-active sanitary landfill leachate. Heavy metal resistance test was conducted for Hg, Cd, Pb, Mg, Zn, Fe, Mn, and Cu (0–20 mg L− 1). The removal of the most toxic heavy metals by the most resistant bacteria was also determined at different salinity levels, i.e., fresh water (0‰), marginal water (10‰), brackish water (20‰), and saline water (30‰). Results showed that the growth of these bacteria is promoted by Fe, Mn, and Cu, but inhibited by Hg, Cd, Pb, Mg, and Zn. The minimum inhibitory concentration (MIC) of all the bacteria in Fe, Mn, and Cu was > 20 mg L− 1. The MIC of V. damsela was 5 mg L− 1 for Hg and >  20 mg L− 1 for Cd, Pb, Mg, and Zn. For P. aeruginosa, MIC was > 20 mg L− 1 for Cd, Pb, Mg, and Zn and 10 mg L− 1 for Hg. Meanwhile, the MIC of P. stutzeri was > 20 mg L− 1 for Pb, Mg, and Zn and 5 mg L− 1 for Hg and Cd. The MIC of P. fluorescens for Hg, Pb, Mg, and Zn was 5, 5, 15, and 20 mg L− 1, respectively, and that for Cd was > 20 mg L− 1. From the MIC results, Hg is the most toxic heavy metal. In marginal water (10‰), P. aeruginosa FZ-2 removed up to 99.7% Hg compared with that in fresh water (0‰), where it removed only 54% for 72 h. Hence, P. aeruginosa FZ-2 is the most resistant to heavy metals, and saline condition exerts a positive effect on bacteria in removing Hg.

scholarly journals Association of Antibiotic and Heavy Metal Resistant Bacteria Screened from Wastewater

2018 ◽  
Vol 7 (1) ◽  
pp. 28-40
Author(s):  
Bikram Gautam ◽  
Rameshwar Adhikari
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Antibiotic Resistance ◽  
Treatment Plant ◽  
Resistance Pattern ◽  
Resistant Bacteria ◽  
Grab Sampling ◽  
Antibiotic Resistance Pattern ◽  
Antibiotic Susceptibility Test ◽  
Heavy Metal Resistant

Wastewater treatment plant is a potential reservoir contributing to the evolution and spread of heavy metal and antibiotic resistant bacteria. The pollutants such as biocides, antibiotics, heavy metals are to be feared for as they have been known to evoke resistance in microorganisms in such polluted environment. The aim of this study was to the isolate bacteria from the treated wastewater and assess the resistance pattern of the isolates against antibiotics and heavy metals. Grab sampling was performed from April to June 2017, from the treated effluent from the secondary treatment plant. To assess the resistance pattern for antibiotic(s) and heavy metal(s), antibiotic susceptibility test and minimum inhibitory concentration by cup well method were performed respectively. Staphylococcus aureus, Enterococcus faecalis, Citrobacter freundii, Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, P. vulgaris, Salmonella Typhi, Pseudomonas aeruginosa were isolated. Multi drug and heavy metal resistant isolates were screened. Fisher’s exact test revealed that there is a significant association (p< 0.001) between antibiotic resistance pattern and resistance patterns at dilution of 2500 g/L (25%). Cramer’s V test revealed that the effect size of antibiotic resistance pattern and heavy metal resistance pattern at dilution 2500 g/L is medium. P. aeruginosa was able to resist the metal concentration up to 10000 g/L (100%) dilution of Fe++. Heavy metal resistant bacteria can be safely used to lower chemical concentration in the environment once their harmful genes are edited, knocked etc. so that risks of evoking antibiotic resistance could be minimized. 

scholarly journals CHARACTERIZATION OF BACTERIA ASSOCIATED WITH PNEUMONIA IN BLACK BENGAL GOATS

2012 ◽  
Vol 9 (1) ◽  
pp. 67-71 ◽  
Author(s):  
MA Momin ◽  
MA Islam ◽  
MM Khatun ◽  
MM Rahman ◽  
MA Islam
Keyword(s):  
Antimicrobial Agents ◽  
Research Work ◽  
Nutrient Agar ◽  
Blood Agar ◽  
Biochemical Tests ◽  
Highly Sensitive ◽  
Golden Yellow ◽  
Agar Media ◽  
Staphylococcus Spp

The present research work was undertaken for the characterization of the bacterial pathogens responsible for pneumonia in black Bengal goats. Nasal swab samples (n = 50) were collected from the pneumonic black Bengal goats in Mymensingh and Sirajgonj districts. Samples were inoculated onto nutrient agar, eosin methylene blue (EMB) agar, MacConkey agar, and blood agar media for isolation of bacteria. Identification of bacteria was performed by the Gram's staining method, cultural properties and biochemical tests. Antibiotic sensitivity of bacterial isolates was performed against 11 antimicrobial agents. Pasteurella spp were isolated from 25 cases, and Staphylococcus spp from 13 cases. Mixed infection caused by the Pasteurella spp and Staphylococcus spp. were recorded in 12 cases. Pasteurella spp produced whitish, opaque circular and translucent colonies on nutrient agar, smooth, convex, glistening colonies on EMB agar and no hemolysis on blood agar. Staphylococcus spp have shown gray white or golden yellowish colonies on  nutrient agar. Golden yellow colonies without hemolysis or whitish colonies with hemolysis were also produced by Staphylococcus spp. on the blood agar media. Pasteurella spp were indole positive, MR-VP negative and ferment dextrose, sucrose and mannitol with the production of acid. The Staphylococcus spp were positive to MR-VP, coagulase and catalase reactions, negative to indole test and fermented five basic sugars with acid production. Results of cultural and biochemical tests supported that these two isolates belonged to P. multocida and S. aureus. P. multocida were highly sensitive to ciprofloxacin and resistant to penicillin. S. aureus found to be highly sensitive to erythromycin, tetracycline, enrofloxacin, and norfloxacin and less sensitive to amoxicillin. DOI = http://dx.doi.org/10.3329/bjvm.v9i1.11215Bangl. J. Vet. Med. (2011). 9(1): 67-71 

scholarly journals Coal extraction causes sediment toxicity in aquatic environments in Santa Catarina, Brazil

2017 ◽  
Vol 12 (4) ◽  
pp. 591 ◽  
Author(s):  
Lucimaira Amaral de Freitas ◽  
Cassiano Lazarotto Rambo (in memoriam) ◽  
Francini Franscescon ◽  
Antônio Felipe Primon de Barros ◽  
Guilherme Dos Santos De Lucca ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Dissolved Oxygen ◽  
Allium Cepa ◽  
Chromosomal Aberrations ◽  
Synergistic Effects ◽  
Sediment Toxicity ◽  
Negative Control ◽  
Coal Extraction ◽  
Santa Catarina ◽  
Sediment Samples

This study evaluated water parameters in ponds affected by coal extraction. Allium cepa assay was used to measure genotoxicity/mutagenicity of the sediment. Samples were collected from four ponds in the southern state of Santa Catarina. Water temperature, pH, dissolved oxygen, conductivity and turbidity were measured. Sediments were analyzed for heavy metals. Elutriate samples were prepared at a ratio of 1:4 sediment:water. Allium cepa bulbs were placed in samples prepared from each pond, with ultrapure water used as negative control and methyl methane sulfonate as positive control. Root length, mitotic index, chromosomal aberrations, micronuclei, and nuclear abnormalities were measured. The pH of two ponds, as well as electrical conductivity and dissolved oxygen of all ponds were below the minimum limits set by Brazilian regulation. All heavy metals analyzed were found in all sediment samples, but only Cd concentration was above the legal limit set by Brazilian law. Allium cepa root growth for samples from Ponds 1, 2, and 4 was significantly lower than the negative control. Meristematic cells exposed to elutriate samples showed no significant changes in cell division. There was a significant increase in total chromosomal aberrations in all treated samples in comparison with the negative control. This study demonstrates that even low concentrations of heavy metals can damage exposed biota, possibly due to synergistic effects. We also found the A. cepa bioassay to be a simple and useful tool for genotoxicity/mutagenicity analyses, and recommend its use for environmental monitoring and management in areas influenced by mining activities.

scholarly journals Cadmium (Cd) Resistance of Isolate Bacteria from Poboya Gold Mining in Palu, Central Sulawesi

2020 ◽  
Vol 20 (2) ◽  
pp. 298
Author(s):  
Fahruddin Fahruddin ◽  
Syahruddin Kasim ◽  
Eka Ummi Rahayu
Keyword(s):  
Heavy Metals ◽  
Mining Industry ◽  
Plate Count ◽  
Resistance Testing ◽  
Bacterial Isolates ◽  
Bacterial Isolation ◽  
Cadmium Resistance ◽  
Cadmium Metal ◽  
Plate Count Method ◽  
Central Sulawesi

The mining industry in Indonesia is growing rapidly, giving an environmental impact from the waste produced because it contains dangerous heavy metals, one of which is cadmium metal (Cd). The purpose to know the ability of cadmium resistance in bacterial isolates. Bacterial isolates were obtained from soil samples from the Poboya Gold Mine field, Palu. Bacterial isolation and selection were carried out by the plate count method, resistance testing using nutrient broth media containing heavy metals Cd, analysis of Cd concentrations using atomic absorption spectrophotometers. From the results of the study, four types of Cd resistant isolates were obtained based on macroscopic and microscopic characterization results, namely isolates EK1, EK2, EK3 and EK4. The two best isolates of Cd resistance, EK2 and EK4 isolates were tested on media containing 20 ppm Cd, showing that both of these isolates were able to significantly reduce Cd concentrations in the supernatant and pellets.

scholarly journals Isolation and Characterization of Pb Resistant Bacteria from Cilalay Lake, Indonesia

2015 ◽  
Vol 4 (3) ◽  
Author(s):  
Kesi Kurnia ◽  
Nina Hermayani Sadi ◽  
Syafitri Jumianto
Keyword(s):  
Heterotrophic Bacteria ◽  
Water Environment ◽  
Resistant Bacteria ◽  
Bacterial Isolates ◽  
Gram Negative ◽  
Isolation And Characterization ◽  
Agar Media ◽  
Physical And Chemical ◽  
Standard Disk

<span>Pollution of water environment with heavy metals is becoming one of the most severe environmental and human health hazards. Lead (Pb) is a major pollutant and highly toxic to human, animals, plants, and microbes. </span><span lang="IN">Toxic metals are difficult to remove from the environment, since they cannot be chemically or biologically degraded and are ultimately indestructible. Biological approaches based on metal-resistant microorganisms have received a great deal of attention as alternative remediation processes. </span><span>This study aim to isolat</span><span lang="IN">e</span><span> and characterize Pb resistant of heterotrophic bacteria in Cilalay Lake, </span><span lang="IN">West Java, </span><span>Indonesia. The water samples were collected </span><span lang="IN">along</span><span> three points around Cilalay Lake. </span><span lang="IN">Water physical and chemical </span><span>determination was performed using the Water Quality Checker</span><span lang="IN">. </span><span>The bacterial isolates were screened on T</span><span lang="IN">r</span><span>ipton</span><span lang="IN">e</span><span> Glucose Yeast (TGY) agar plates. </span><span lang="IN">Afterwards s</span><span>elected isolates were grown on Nutrient Agar media 50% </span><span lang="IN">with </span><span>supplemented Pb 100 ppm by the standard disk. Population of resistant bacteria was counted. The result from metal resistant bacteria indicated that all isolates w</span><span lang="IN">ere</span><span> resistant. The most abundant type of resistant </span><span lang="IN">bacteria </span><span>to lead was Gram negative more than Gram positive. Identified have metal resistant bacteria could be useful for the bioremediation of heavy metal contaminated sewage and waste water</span>

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