scholarly journals Metabolic and functional cardiac changes at II-type pancreatic diabetes and abdominal adiposity in rats

2003 ◽  
Vol 2 (3) ◽  
pp. 30-36
Author(s):  
M. V. Kolbina ◽  
V. T. Dolgikh ◽  
V. I. Chesnokov
Keyword(s):  
Carbohydrate Metabolism ◽  
Contractile Function ◽  
Negative Inotropic Effect ◽  
Glycated Haemoglobin ◽  
Control Group ◽  
Metabolic Disturbances ◽  
Functional Abnormality ◽  
Heart Metabolism ◽  
Pancreatic Diabetes ◽  
Leading Role

Investigation of pathogenetic correlation of abdominal adisposity and II-type pancreatic diabetes (PD) has been made with the aim to reveal the importance of carbohydrate metabolism disturbances at above said pathology combination in cardiac abnormalities. 30 white alley rats at the age of 8-12 months have been included into the experimental group. Control group has been formed of 30 animals. Used methods of investigation: simulation of II-type PD in rats with streptozotocin and study of carbohydrate metabolism indices in entire organism as well as contractile function indices and indices of isolated and contracting heart metabolism. As a result it has been revealed that the weight of rats with II-type PD and abdominal adisposity, the level of glucose and glycated haemoglobin in blood, of lactate and pyruvate had been increased surely. The normal level of blood serum C-peptide has confirmed the absence of mass death of β-cells. The hearts taken from diabetic animals have responded to the increase of contraction frequency with the decrease of advanced pressure, i.e. the negative inotropic effect has been observed. Therefore the peripheral insulin resistance plays the leading role in the development of metabolic and functional abnormality complex at II-type PD and abdominal adisposity. Accumulation of lactate, metabolic acidosis, decrease of glucose efficiency and dysfunction of cardiac hystiocyte calcium pump with the development of diastolic myocardium dysfunction contribute to the development of metabolic disturbances.

scholarly journals VITAMIN D AND DIABETES MELLITUS TYPE 2

2017 ◽  
Author(s):  
N. V. Pasyechko ◽  
V. M. Kulchinska ◽  
L. V. Radetska ◽  
L. V. Naumova ◽  
I. V. Smachylo ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Vitamin D ◽  
Carbohydrate Metabolism ◽  
Diabetes Mellitus Type 2 ◽  
Glycated Haemoglobin ◽  
Diabetes Mellitus Type ◽  
Control Group ◽  
Metabolic Disturbances ◽  
Patients With Diabetes

Background. As it is established that diabetes mellitus causes metabolic disturbances of all types (first of all because of carbohydrate metabolism), affection of blood vessels, nerves, different organs and tissues. However, the influence of DM on the level of microelements is still not investigated enough. Despite the sufficient variety of medicaments, compensation of diabetes mellitus is achieved in less than 30% of patients. For this reason, the search of new ways of treatment which are aimed at the improvement of carbohydrate metabolism is an urgent issue.Objective. The research was aimed to reveal the deficiency of 25-hydroxyvitamin D3 [25(OH)D3] and its correlation with carbohydrate metabolism.Materials and methods. Thirty five patients with diabetes mellitus Type 2 aged 55-74 with illness duration 2-4 years were examined. The control group included 35 healthy people of the same age and sex. Levels of 25-hydroxyvitamin D3 [25(OH)D3] were tested by means of radioimmunoassay. The level of glycated haemoglobin was tested by means of liquid chromatography.Results. Correlation of the level of vitamin D with the degree of diabetes mellitus Type 2 compensation has been detected.Conclusions. A clear tendency to the improvement of diabetes mellitus Type 2 compensation after medication correction with vitamin D was evidenced. 

Nerve-sparing radical hysterectomy in patients with infiltrative cervical cancer

2016 ◽  
pp. 46-51
Author(s):  
T. Dermenzhy ◽  
◽  
V. Svintitskiy ◽  
S. Nespryadko ◽  
L. Legerda ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Radical Hysterectomy ◽  
Contractile Function ◽  
Major Elements ◽  
Control Group ◽  
Nerve Sparing ◽  
Group I ◽  
Hypogastric Plexus ◽  
Prognostic Indexes

The objective: to improve an effectiveness of therapy and quality of life of patients with infiltrative cervical cancer using radical hysterectomy accomplished with nerve-sparing methodology. Patients and Methods: Ninety patients with histologically verified infiltrative cervical cancer were cured with radical hysterectomy (RHE) in the Department of Oncogynecology of National Cancer Institute (Kyiv, Ukraine) in 2012-2016. The age of the patients was from 26 to 65 years (an average age of 42.61±1.06). The patients were distributed in 2 groups: group I treated with nerve-sparing radical hysterectomy (NSRHE), 45 patients, the main group; group II treated with radical hysterectomy (RHE III), the control group, 45 patients. The prognostic indexes in the groups were similar. Results. NSRHE that included the dissection of cardinal ligament, separation of dorsal and anterior layers of uterovesical ligament allowed separate uterine branch of inferior hypogastric plexus, preserve an innervation of urinary bladder and prevent the malfunction of its contractile function at postoperative period. Conclusion. The data of the urodynamic study using cystomanometry performed at pre- and early operative periods have shown that surgical treatment of patients with infiltrative cervical cancer with preservation of the major elements of pelvic autonomic plexuses allows significantly decrease the rate of postoperative urogenical malfunctions. Key words: nerve-sparing radical hysterectomy, cervical cancer, cystomanometry.

Effects ofS-nitroso-N-acetylcysteine on contractile function of reperfused skeletal muscle

1998 ◽  
Vol 274 (3) ◽  
pp. R822-R829 ◽  
Author(s):  
Long-En Chen ◽  
Anthony V. Seaber ◽  
Rima M. Nasser ◽  
Jonathan S. Stamler ◽  
James R. Urbaniak
Keyword(s):  
Skeletal Muscle ◽  
Reperfusion Injury ◽  
Contractile Function ◽  
Ischemia Reperfusion ◽  
Protective Role ◽  
Control Group ◽  
Group Vi ◽  
No Donor ◽  
Early Reperfusion ◽  
Reconstructive Operations

The ultimate goal of replantation and microsurgical reconstructive operations is to regain or improve impaired function of the tissue. However, the data related to the influence of NO on tissue function are limited. This study evaluated the effects of the NO donor S-nitroso- N-acetylcysteine (SNAC) on contractile function of skeletal muscle during reperfusion. Forty-nine rats were divided into six groups. The extensor digitorum longus (EDL) muscles in groups I and II were not subjected to ischemia-reperfusion but were treated with a low (100 nmol/min) or high (1 μmol/min) dose of SNAC. In groups III- V, the EDL underwent 3 h of ischemia and 3 h of reperfusion and was also treated with low (100 nmol/min) or high doses (1 or 5 μmol/min) of SNAC. Group VI was a phosphate-buffered saline (PBS)-treated control group. Twenty additional animals were used to document systemic effects of SNAC and PBS only. SNAC or PBS was infused for 6.5 h, beginning 30 min before ischemia and continuing throughout the duration of reperfusion. Contractile testing compared the maximal twitch force, isometric tetanic contractile forces, fatigue, and fatigue half time of the experimental EDL and the contralateral nontreated EDL. The findings indicate that 1) SNAC does not influence contractile function of EDL muscle not subjected to ischemia-reperfusion, 2) SNAC significantly protects the contractile function of ischemic skeletal muscle against reperfusion injury in the early reperfusion period, and 3) the protective role of SNAC is critically dosage dependent; protection is lost at higher doses. The conclusion from this study is that supplementation with exogenous NO exerts a protective effect on the tissue against reperfusion injury.

scholarly journals Selective TNF-α targeting with infliximab attenuates impaired oxygen metabolism and contractile function induced by an acute exposure to air particulate matter

2015 ◽  
Vol 309 (10) ◽  
pp. H1621-H1628 ◽  
Author(s):  
Timoteo Marchini ◽  
Verónica D'Annunzio ◽  
Mariela L. Paz ◽  
Lourdes Cáceres ◽  
Mariana Garcés ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Saline Solution ◽  
Contractile Function ◽  
Left Ventricular ◽  
Acute Exposure ◽  
Control Group ◽  
Contractile Reserve ◽  
Mice Model ◽  
Cardiovascular Morbidity And Mortality ◽  
Tnf Α

Inflammation plays a central role in the onset and progression of cardiovascular diseases associated with the exposure to air pollution particulate matter (PM). The aim of this work was to analyze the cardioprotective effect of selective TNF-α targeting with a blocking anti-TNF-α antibody (infliximab) in an in vivo mice model of acute exposure to residual oil fly ash (ROFA). Female Swiss mice received an intraperitoneal injection of infliximab (10 mg/kg body wt) or saline solution, and were intranasally instilled with a ROFA suspension (1 mg/kg body wt). Control animals were instilled with saline solution and handled in parallel. After 3 h, heart O2 consumption was assessed by high-resolution respirometry in left ventricle tissue cubes and isolated mitochondria, and ventricular contractile reserve and lusitropic reserve were evaluated according to the Langendorff technique. ROFA instillation induced a significant decrease in tissue O2 consumption and active mitochondrial respiration by 32 and 31%, respectively, compared with the control group. While ventricular contractile state and isovolumic relaxation were not altered in ROFA-exposed mice, impaired contractile reserve and lusitropic reserve were observed in this group. Infliximab pretreatment significantly attenuated the decrease in heart O2 consumption and prevented the decrease in ventricular contractile and lusitropic reserve in ROFA-exposed mice. Moreover, infliximab-pretreated ROFA-exposed mice showed conserved left ventricular developed pressure and cardiac O2 consumption in response to a β-adrenergic stimulus with isoproterenol. These results provides direct evidence linking systemic inflammation and altered cardiac function following an acute exposure to PM and contribute to the understanding of PM-associated cardiovascular morbidity and mortality.

scholarly journals The relationship of sportsmanship and components of the training load of students-athletes (for example, middle-distance running).

2019 ◽  
Vol 5 (1) ◽  
pp. 84
Author(s):  
N.І. Neporadna
Keyword(s):  
Physical Education ◽  
Physical Fitness ◽  
Respiratory System ◽  
Physical Development ◽  
Training Load ◽  
Movement Speed ◽  
Control Group ◽  
Preliminary Training ◽  
Leading Role ◽  
Physical Abilities

<p><em>The purpose of the work</em><strong> </strong>is to identify the influence of various components of the training load on the improvement of sportsmanship of athletes at the stages of preliminary training and initial specialization. <em>Research methods</em>. Students at the age of 17, who were engaged in the athletics section and performed regulatory tests at the level of the third sports category, were divided into 2 groups: experimental and control (10 people each). The students of the experimental group were trained in the author's 6-component training load program. The students of the control group were engaged in a program developed at the Research Institute of Physical Education and Sport. Investigated indicators of physical development (height, body weight, functional reserves of the cardio-respiratory system) and physical fitness (endurance, speed and power indicators) every 6 months for 5 years. <em>Results</em>. The developed program, which is implemented at the stages of preliminary training and initial specialization of athletes, provides for the development of specially oriented physical abilities and allows you to increase the level of sportsmanship. This is indicated by the dynamics of the obtained positive results of testing basic anthropometric data and indicators of physical training of students-athletes. It is also necessary to take into account the direction of development of physical abilities, based on the development of the main indicators of the cardio-respiratory system and the leading role of the mechanisms influencing the regulation of the level of general endurance, movement speed and strength characteristics in the whole organism. The program provides for a systematic, but gradual complication of physical activity, determines the appropriate conditions for the implementation of the basic principle of physical education in higher educational institutions and developmental training for students-athletes of various qualifications. <em>Conclusion</em>. The results of 5-year testing of students-athletes after the introduction of the author's program in the training process, indicate pronounced positive changes in physical development and physical fitness, expressed in increasing the level of their sportsmanship and achievement of sports results corresponding to the first sports category.</p>

scholarly journals Adiponutrin as a Marker of Metabolic Disorders in Patients with Hypertension with Overweight and Obesity

2021 ◽  
Vol 6 (2) ◽  
pp. 77-86
Author(s):  
P. G. Kravchun ◽  
◽  
O. I. Kadykova ◽  
U. S. Herasymchuk
Keyword(s):  
Enzyme Immunoassay ◽  
Carbohydrate Metabolism ◽  
Metabolic Disorders ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Body Mass Index ◽  
Vascular Cognitive Impairment ◽  
Arterial Disease ◽  
Overweight And Obesity ◽  
Control Group

Hypertension is a leading risk factor for coronary heart disease, stroke, peripheral arterial disease, kidney disease, vascular cognitive impairment, and mortality worldwide. Obesity in combination with hypertension continues to attract considerable attention from scientists. Despite the presence of experimental and clinical evidence of mutual burdening of hypertension and obesity, the mechanisms of formation of pathophysiological consequences of such a combination remain insufficiently defined. That is why in recent years an important role is given to the adiponutrin protein. The purpose of the study is to assess the nature of the relationship between adiponutrin levels and lipid and carbohydrate metabolism depending on the duration and degree of hypertension and the degree of obesity in patients with this comorbid pathology. Material and methods. The study included 107 patients with hypertension who were hospitalized in the cardiology department of Municipal noncommercial enterprise "Kharkiv City Clinical Hospital No.27" of Kharkiv municipal council. The age of patients ranged from 32 to 79 years (mean age was 58.6±9.88), who had not previously received regular antihypertensive therapy. The control group consisted of 20 age- and sex-appropriate healthy individuals. The level of adiponutrin was determined in 58 patients with hypertension of the 1st - 3rd degrees, aged from 32 to 79 years (mean age was 57.5±10.11 years), while hypertension of the 1st degree was diagnosed in 12 (20.7%) patients, hypertension of the 2nd degree - in 16 (27.6%), and hypertension of the 3rd degree - in 30 (51.7%) of the examined patients. Blood pressure was measured in patients in a sitting position after 5 minutes of rest, anthropometric measurements were performed, lipid profile and indicators of carbohydrate metabolism were evaluated, adiponutrin levels (pg/mL) were determined by enzyme immunoassay method using The RayBio® Adiponutrin Enzyme Immunoassay (EIA) Kit, (USA). Results and discussion. In all groups of patients with hypertension with overweight and obesity, adiponutrin levels were significantly elevated, and lipid and carbohydrate profiles tended to increase. Significant direct correlations were found between adiponutrine and triglycerides, with very low-density lipoprotein cholesterol of a moderate nature, and draws attention with a very high body mass index. In considering the differences in adiponutrin levels depending on gender, it was found that men have higher adiponutrin levels than women. Conclusion. Thus, we have the opportunity to consider adiponutrin as a marker of metabolic disorders

scholarly journals Dysmetabolic mechanisms of preeclampsia development

2021 ◽  
Vol 17 (4) ◽  
pp. 346-356
Author(s):  
I. S. Lipatov ◽  
Yu. V. Tezikov ◽  
A. R. Azamatov
Keyword(s):  
Insulin Resistance ◽  
Pregnant Women ◽  
Metabolic Disorders ◽  
Hematological Parameters ◽  
Control Group ◽  
Metabolic Disturbances ◽  
Group Iii ◽  
Inflammatory Status ◽  
Group I

Background: An in-depth study of dismetabolic mechanisms in the genesis of pre-eclampsia (PE) has been updated because pregnancy is considered as a natural model of metabolic syndrome (MS), as well as the metabolic disorders are important in development of essential hypertension.Aims: to reveal clinical and laboratory parallels in pregnancy complicated by PE without MS and pregnancy proceeding on the background of MS to assess the role of metabolic disturbances in the development of PE.Materials and methods: 82 women with MS were examined in the dynamics of pregnancy and were divided into 2 groups depending on the implementation of PE: group I consisted of 50 women with PE on the background of MS, group II 32 women with MS without PE. We formed group III consisting of 44 pregnant women with PE without accompanying diseases to assess the pathogenetic value of metabolic disorders in the development of PE. The IV (control) group consisted of 30 healthy women with physiological pregnancy. Metabolic, hematological parameters, hormones, markers of the proinflammatory state, endothelial hemostasiological dysfunction, decidualization and placental angiogenesis, accumulation dynamics and distribution loci of adipose tissue were determined in all pregnant women.Results: In the groups of pregnant women with PE, changes similar to MS were revealed: pronounced diabetic and atherogenic disorders with the development of pathological insulin resistance, hyperinsulinemia and leptinemia, endothelial-platelet link hyperactivation, thrombotic and inflammatory status, visceral type of fat deposition, hyperuricemia, hypersympathicotonia. It is proved that in the hierarchy of mechanisms of PE formation, placental dysfunction is a secondary alteration factor, which additionally potentiates the insulin resistance increase and the effects of structural and functional destabilization of the vascular endothelium.Conclusions: The direction of metabolic changes during pregnancy, the common development of PE and MS indicate the important role of dismetabolic mechanisms in the formation of PE.

scholarly journals The Diagnostic Value of the Glucose Curve in Individuals with and without Diabetes

2019 ◽  
pp. 1-7
Author(s):  
Mohamed S. El-Gareb ◽  
Mohamed N. El-Naggar
Keyword(s):  
Blood Sample ◽  
Glycated Haemoglobin ◽  
Diagnostic Value ◽  
Control Group ◽  
Diabetic Patients ◽  
Measured Concentration ◽  
Normal Individuals ◽  
Edta Tube ◽  
Time Of Administration ◽  
Glucose Curve

Aim: Of this study is to demonstrate the importance of glucose curve test in monitoring pre and post-meal variation in diabetic and normal individuals. Methodology: The individuals subjected to this study mainly grouped in two categories the (DM2 group) and the (Control group), they instructed to came fasting at which blood sample will be collected in EDTA and blank tube then after 30 min. the first post-prandial blood sample collected and then after every 1,2,3,4,5,6,7,8 hours blood sample collected subsequently, then serum separated from each sample (except the EDTA tube) analysed biochemically for glucose and glycated haemoglobin HbA1c (from EDTA tube). Result: We found that, the calculated glucose based on mean glycated haemoglobin HbA1c% results underestimate the real concentrations all over the glucose curve in control group but in DM2 group it underestimate the mean and some actually measured concentration in some points of the curve which adds more burden on the diabetic patient and the responsibility of adjusting the dose and time of administration. Conclusion: from our prospect we recommend the use of blood glucose curve as a monitoring and diagnostic tool generally for glucose metabolism in normal, pre-diabetic, diabetic and uncontrolled diabetic patients before and during therapeutic conditions.

scholarly journals SUPERFICIAL CANDIDOSIS COURSE IN PATIENTS WITH CARBOHYDRATE METABOLISM DISORDER AND DIABETES MELLITUS

2019 ◽  
Vol 72 (1) ◽  
pp. 68-71
Author(s):  
Оrysya О. Syzon ◽  
Solomiya A. Turkevych ◽  
Tetyana I. Rudnyk ◽  
Svitlana V. Volbyn ◽  
Marianna O. Dashko
Keyword(s):  
Diabetes Mellitus ◽  
Carbohydrate Metabolism ◽  
Glucose Tolerance Test ◽  
Glycosylated Hemoglobin ◽  
Tolerance Test ◽  
Control Group ◽  
Clinical Tests ◽  
Metabolism Disorder ◽  
Mucous Membranes ◽  
Reliable Detection

Introduction: One of the pressing issues of modern dermatology is the prevalence of diseases of skin and mucous membranes caused by yeast-like fungi. The aim: To study specific features of the course of skin and mucous membranes superficial candidosis against the background of initial carbohydrate metabolism disorders or diabetes mellitus. Materials and methods: We have examined 93 patients aged 18-75 suffering from superficial candidosis. Candidosis was diagnosed based on anamnesis data, results of clinical tests and laboratory studies. The study of carbohydrate metabolism included determining of the glucose level under fasting conditions to establish possible diabetes mellitus and glucose tolerance test (75 g glucose load), glycosylated hemoglobin (HbA1c) and fructosamine (FR). Results: In patients with mild and moderate candidosis we have detected an increased rates of НbA1с and fructosamine, as compared with the like indicators received in the control group, by 1.54 and 1.21 times respectively. In patients with severe candidosis and relapses the concentrations of НbA1с increased by 2.59 times, FR – by 2.26 times against the indicators received in the control group. Thus, increased levels of blood glycosylated protein indicate the pathogenetic importance of these processes in the development of candidosis. Conclusions: Consequently, increased levels of blood glycosylated protein indicates the pathogenetic importance of these processes in the development of candidal lesions. Tests for НbA1с and FR proved to be sensitive and allow reliable detection of individuals with diabetes mellitus and glucose intolerance among candidosis patients.

scholarly journals Proinflammatory Cytokines Are Soluble Mediators Linked with Ventricular Arrhythmias and Contractile Dysfunction in a Rat Model of Metabolic Syndrome

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Evaristo Fernández-Sada ◽  
Alejandro Torres-Quintanilla ◽  
Christian Silva-Platas ◽  
Noemí García ◽  
B. Cicero Willis ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Ventricular Arrhythmias ◽  
Mechanical Performance ◽  
Contractile Function ◽  
Ex Vivo ◽  
Heart Function ◽  
Control Group ◽  
Serum Triglycerides ◽  
Rat Cardiomyocytes ◽  
Proinflammatory Mediators

Metabolic syndrome (MS) increases cardiovascular risk and is associated with cardiac dysfunction and arrhythmias, although the precise mechanisms are still under study. Chronic inflammation in MS has emerged as a possible cause of adverse cardiac events. Male Wistar rats fed with 30% sucrose in drinking water and standard chow for 25–27 weeks were compared to a control group. The MS group showed increased weight, visceral fat, blood pressure, and serum triglycerides. The most important increases in serum cytokines included IL-1β(7-fold), TNF-α(84%), IL-6 (41%), and leptin (2-fold), the latter also showing increased gene expression in heart tissue (35-fold). Heart function ex vivo in MS group showed a decreased mechanical performance response to isoproterenol challenge (ISO). Importantly, MS hearts under ISO showed nearly twofold the incidence of ventricular fibrillation. Healthy rat cardiomyocytes exposed to MS group serum displayed impaired contractile function and Ca2+handling during ISO treatment, showing slightly decreased cell shortening and Ca2+transient amplitude (23%), slower cytosolic calcium removal (17%), and more frequent spontaneous Ca2+release events (7.5-fold). As spontaneous Ca2+releases provide a substrate for ventricular arrhythmias, our study highlights the possible role of serum proinflammatory mediators in the development of arrhythmic events during MS.

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