scholarly journals Selection of Rhizosphere Bacterial Isolates and Development of Antagonistic Bacterial-Based Formulation to Control Fusarium Wilt on Shallot

2020 ◽  
Vol 26 (2) ◽  
pp. 89
Author(s):  
Yadi Suryadi ◽  
Dwiningsih Susilowati ◽  
I Made Samudra

<p>Wilt disease caused by Fusarium spp. is a major disease in shallot-producing areas particularly in the North Coast of West Java. The research was aimed to select rhizosphere bacteria and develop an antagonistic bacterial-based formulation for its effectiveness to suppress wilt disease caused by Fusarium spp. on shallot plant under greenhouse and field trials. The field trials were carried out using randomized complete block design with four replications at the shallot farmer condition in Subang and Indramayu during the 2019 planting season. Antagonistic test of bacterial isolates from the rhizosphere showed that most of the bacterial isolates can be used as a biocontrol to Fusarium spp. pathogen. The result revealed that bacterial suspension formulation (E-76 + DBS-2 isolates) in combination with phytohormone (1:1 [v/v]) at Subang and Indramayu tended to suppress Fusarium wilt disease intensity with the disease inhibition ranging from 73.54–93.39% and 66.3–95.65%, respectively. The spraying application of formulation 2.5 ml/l was obtained as the best formula to suppress the disease. The growth and production of shallot were also affected by the application of antagonistic-bacterial formula.</p>

AgriPeat ◽  
2019 ◽  
Vol 20 (01) ◽  
pp. 19-26
Author(s):  
Admin Journal

ABSTRACTThe role of Trichocompost and KCl fertilizer to control Fusarium wilt disease on onion in sandy soil. Fusarium wilt on onion is an interesting disease it is can loss the onion yield. The purpose of research to study trichocompost and KCl fertilizer role to control Fusarium wilt disease on ann onion. The research design used a Factorial Randomized Block Design with 2 factors. The first factor is 4 levels trichocompost, it is: without trichocompost (T0), trichocompost 10 t.ha-1 dosage (T1), trichocompost 20 t.ha-1 dosage (T2), trichocompost 30 t.ha-1 dosage (T3). The second factor is 3 levels KCl fertilizer, it is: without KCl (K0), KCl 100 kg.ha-1 dosage (K1), KCl 200 kg.ha-1 dosage (K2). Result of this research showed the application of trichocompost 10 t.ha-1 dosage and KCl 100 kg.ha-1 dosage can inhibit Fusarium wilt incubation time, can inhibit the patogen development with effective value 89,23%, the single factor it is aplication trichocompost 10 t.ha-1 dosage and trichocompost 30 t.ha-1 dosage not significant to dried onion bulb weight per clump of onion plant.Key words: Trichocompost, KCl fertilizer, Fusarium wilt disease, onion, sandy soil.ABSTRAKPenyakit layu Fusarium merupakan salah satu penyakit penting dapat menurunkan produksi bawang merah hingga 50%. Tujuan penelitian untuk mengetahui peranan trichokompos dan pupuk KCl dalam mengendalikan penyakit layu fusarium pada tanaman bawang merah. Penelitian menggunakan Rancangan Acak Kelompok faktorial dua faktor perlakuan. Faktor pertama 4 taraf dosis trichokompos yaitu: tanpa trichokompos (T0), trichokompos dosis 10 t.ha-1 (T1), trichokompos dosis 20 t.ha-1 (T2), trichokompos dosis 30 t.ha-1 (T3). Faktor kedua 3 taraf dosis pupuk KCl yaitu: tanpa pupuk KCl (K0), pupuk KCl dosis 100 KCl kg.ha-1 (K1), pupuk KCl dosis 200 KCl kg.ha-1 (K2). Hasil penelitian menunjukkan pemberian trichokompos 10 t.ha-1 dan pupuk KCl 100 kg.ha-1 dapat memperpanjang masa inkubasi penyakit, menekan serangan penyakit layu Fusarium dengan nilai efektivitas sangat baik (89,23%), perlakuan tunggal trichokompos dosis 10 t.ha-1 tidak berbeda nyata dengan dosis 30 t.ha-1 terhadap bobot umbi kering per rumpun tanaman bawang merah.Kata kunci: penyakit layu Fusarium, pupuk KCl, tanah berpasir, tanaman bawang merah, trichokompos.


2018 ◽  
Vol 14 (2) ◽  
pp. 14-28
Author(s):  
Bukhari Bukhari ◽  
Nuryulsen Safridar

This research was conducted in Pante Cermin, Padang Tiji Subdistrict, Pidie District. in March to August 2015, with the aim to know the use efficienci of Trichoderma sp on the growth of several types of Banana seedlings (Musa Paracica L.) This research using Randomized Block Design (RAK) factorial pattern with the factors studied is the type of banana and trichoderma dose . Both factors consist of 4 levels: Banana Barangan (J1), Ambon banana (J2), Banana King (J3) and Geupok banana (J4). While the dose of Tricoderma sp is: TO = 0 g / seeds T1 = 15 g / seeds T2 = 30 g / seedlings and T3 = 45 g / seedlings. So there are 16 treatment combinations and repeated 3 times, which resulted in 48 experimental units.To determine the influence of each treatment and its interaction on the growth of banana seedlings, the analysis of variance (Test F) and continued with the test of Beda Nyata Jujur (BNJ) at 5% level.The results showed that the growth of good banana seedlings among 4 species studied was shown by Barangan bananas, but not unlike bananas. However, trichoderma administration until the age of 4 months has not shown the growth and intensity of different attacks. So it should be extended the study period to 6 months. After 6 months of research there has been a difference in growth and intensity of attack, where the intensity of the greatest attack is shown by T0 (without trichoderma). Medium intensity of smallest attack is shown by T3 (Dose trichoderma sp 45 gr / banana seedlings). The type of banana does not affect the growth and intensity of fusarium wilt attack. and the interaction of these two factors had no significant effect on the growth of banana seedlings and the intensity of Fusarium wilt disease.


2013 ◽  
Vol 22 (3) ◽  
pp. 276 ◽  
Author(s):  
Ika Djatnika

<p>ABSTRAK. Layu Fusarium merupakan penyakit penting yang menjadi kendala dalam memproduksi tanaman anggrek. Untuk mengendalikannya,  petani masih menggunakan fungsida. Tanaman anggrek kerap ditampilkan sebagai hiasan yang dekat dengan lingkungan manusia, maka penggunaan pestisida perlu diperhatikan. Oleh karena itu sangat penting dicari cara pengendalian lainnya yang aman terhadap lingkungan, antara lain dengan  pengendalian hayati. Tujuan penelitian ialah mendapatkan isolat bakteri antagonis yang dapat mengendalikan layu Fusarium pada tanaman Phalaenopsis. Percobaan dilakukan di Laboratorium dan Rumah Kasa Balai Penelitian Tanaman Hias Segunung (1100 m dpl.) serta untuk  mikrob diisolasi dari lokasi tanaman hortikultura di Jawa Barat dan DKI Jakarta, mulai Bulan Januari sampai dengan Desember 2010. Penelitian meliputi isolasi Fusarium spp. sebagai patogen pada tanaman anggrek di beberapa lokasi, isolasi bakteri antagonis, uji kemangkusan bakteri terhadap pertumbuhan Fusarium spp. di laboratorium, dan uji kemangkusan bakteri antagonis terhadap layu Fusarium di rumah kasa. Hasil penelitian menunjukkan bahwa penyebab layu Fusarium pada tanaman Phalaenopsis ialah Fusarium oxysporum. Dari 154 isolat bakteri yang diisolasi dari lapangan, hanya ada tiga  isolat yaitu nomor B23, B 26, dan B37 yang dapat menekan pertumbuhan F. oxysporum pada media PDA. Sampai dengan pengamatan minggu ke-10 setelah inokulasi, ketiga bakteri tersebut masing-masing menekan jumlah tanaman yang terserang layu Fusarium, yaitu sebesar 46,9; 48,9; dan 65,3%, dan masing-masing menekan intensitas penyakit layu 50,5; 43,9; dan 55,1%.</p><p>ABSTRACT. Djatnika, I 2012. Selection of Antagonistic Bacteria for Controlling of Fusarium Wilt on Phalaenopsis Plants. Fusarium wilt is an important disease as constraint on production of orchid plants. The control of Fusarium wilt of orchids with fungicides often use by farmers. Orchid plants are often displayed as a decoration which is close to the human environment, so the application of pesticides have to get attention. It is therefore necessary to find another method that is safe for environments, such as using of biological control. The purpose of the study was to get isolates of  bacterial antagonists for controlling of Fusarium wilt of Phalaenopsis plants. The experiment was conducted at Laboratory and Screenhouse of Indonesian Ornamental Plant Research Institute, Segunung (1100 m asl.) and the microbes were isolated from horticultural area in West Java and DKI Jakarta since January until December 2010. The research comprised of isolation of Fusarium spp. from orchid plants in some location, isolation of bacterial antagonists, the effectiveness of the bacteria to suppress Fusarium spp. growth in laboratory, and the effectiveness of the bacteria to control Fusarium wilt on Phalaenopsis plants in the screenhouse. The results showed that the causal Fusarium wilt of Phalaenopsis plants was identified as Fusarium oxysporum. Three of 154 isolates of bacteria, i.e. isolates number of B23, B26, and B37, could suppress of F. oxysporum growth on PDA media. Observation up to 10 weeks after inoculation, the three bacteria could reduce the number of  plants attacked by Fusarium wilt , which were 46.9; 48.9; and  65.3% respectively, and each of them suppress wilt disease intensity 50.5, 43.9, and 55.1% respectively. <br /><br /></p>


Jurnal Agro ◽  
10.15575/5397 ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 144-152
Author(s):  
Endang Mugiastuti ◽  
Abdul Manan ◽  
Ruth Feti Rahayuniati ◽  
Loekas Soesanto

Fusarium oxysporum merupakan penyebab penyakit layu fusarium yang dapat menurunkan produksi tomat. Pengendalian hayati dengan menggunakan bakteri antagonis seperti Bacillus sp. merupakan alternatif pengendalian yang potensial dan ramah lingkungan.  Penelitian bertujuan untuk mengetahui kemampuan Bacillus sp. dalam mengendalikan penyakit layu fusarium pada tanaman tomat di lapangan. Percobaan menggunakan rancangan acak kelompok dengan 5 perlakuan dan 5 ulangan, meliputi: kontrol, Bacillus sp. B42, Bacillus sp. B64, gabungan Bacillus sp. B42 dan B64, serta fungisida. Hasil pengujian menunjukkan bahwa Bacillus sp. B.64 merupakan bakteri antagonis terbaik dalam menekan intensitas penyakit  layu fusarium, karena dapat menunda masa inkubasi 15,76%, menekan intensitas penyakit 38,77%, meningkatkan kandungan fenol tanaman (tanin, saponin dan glikosida), serta meningkatkan pertumbuhan dan hasil tanaman dengan meningkatkan bobot kering tajuk 32,05%, bobot kering akar 15,23%, dan bobot buah per tanaman 46,48%.ABSTRACTFusarium oxysporum is the causal agent of fusarium wilt disease which decreases the production of tomatoes. Biological control managenement using bacterial antagonists is a potential alternative to prevent the infection of the disease. The aim of this research was to determine the ability of Bacillus sp. to control tomato fusarium wilt in the field. Randomized block design (RBD) experiment was used consisting of 5 treatments and 5 replications i.e. control, Bacillus sp. B42, Bacillus sp. B64, combination of Bacillus sp B42 + B64, and fungicide. The results showed that Bacillus sp. B64 was the best bacterial antagonist agent to control tomato wilt disease by delaying incubation period (15.76%), decreasing disease intensity (38.77%), increasing phenol compounds (tannin, saponin, glycosides) and improving plant growth and yield. Furthermore, the results showed the increasement of shoot dry weight to 32.05%, root dry weight to 15.23%, and yield to 46.48% as well.


2021 ◽  
Vol 913 (1) ◽  
pp. 012014
Author(s):  
I M Sudantha ◽  
Sudirman ◽  
N M L Ernawati

Abstract Legundi weed (Vitex trifolia) has the potential to be used as a biofungicide to control Fusarium wilt disease on shallots. For the manufacture of biofungoicides, legundi leaf extract was first fermented using the fungus Trichoderma harzianum. The aim of this study was to determine the effect of the method and dose of fermented Legundi leaf extract biofungicide on the Fusarium fungus that causes shallots wilt. The research was carried out in Senteluk Village, Batu Layar District, West Lombok Regency using an experimental method with a factorial randomized block design with two factors. As the main effect, namely the application of biofungicide fermented Legundi leaf extract consisting of two levels, namely: seed treatment before planting, and spraying treatment for plants aged 7 days after planting. Meanwhile, the simple effect is the application dose of fermented Legundi leaf extract biofungicide consisting of 5 levels, namely: 0.00 mL, 2.50 mL; 5.00 mL; 7.50 mL and 10.00 mL. The combination method and dose of fermented Legundi leaf extract biofungicide was repeated three times each, so there were 30 treatment combinations. The results obtained were the application of Legundi leaf extract biofungicides by soaking the seeds before planting at a starting dose of 2.50 mL to effectively control onion wilt disease. The applied biofungicide can increase plant height and increase the dry weight of the harvest.


Agrologia ◽  
2021 ◽  
Vol 10 (2) ◽  
Author(s):  
Rafit Syarifudin ◽  
A.Marthin Kalay ◽  
Costanza Uruilal

Onion (Allium Ascalonicum L) are one of the leading horticultural commodities that have economic value. In cultivation, Fusarium wilt disease is often damaged caused by the fungus Fusarium oxysporum. This study aimed to examine the effect of biofertilizer application on fusarium wilt disease, growth and yield of onion. The treatments tested were Bion-up biofertilizer, KIBRT, Biostimulant, Azoto-Tricho, anthracol fungicide, and without biological fertilizer as a control. Using a randomized block design with three replications. Observation variables were the incidence of fusarium wilt disease, plant height, number and weight of tubers. The results showed that the application of biofertilizers (Bion up, KIBRT, Biostimulants and Azoto-Tricho) was effective in increasing plant height, number of bulbs, and bulb weight of onion bulbs, while controlling the development of fusarium wilt disease was more effective when using Bion up, KIBRT, and Azoto-Tricho.Keywords: Biofertilizer, Antracol, Shallots, Fusarium oxysporum


Plant Disease ◽  
1997 ◽  
Vol 81 (1) ◽  
pp. 66-70 ◽  
Author(s):  
P. D. Colyer ◽  
T. L. Kirkpatrick ◽  
W. D. Caldwell ◽  
P. R. Vernon

Eight cotton cultivars, with and without aldicarb treatment, were evaluated for their effect on the severity of the root-knot nematode-Fusarium wilt disease complex in cotton. A randomized complete block design with a split-plot arrangement of treatments was used with cotton cultivars as the main plots and nematicide treatments as the subplots. Results in 1994 and 1995 were similar. Yield of lint, boll weight, and wilt and root-gall ratings were different among cultivars and between nematicide treatments both years. Increased lint yield and boll weight, and reduced root-gall and wilt ratings were associated with application of aldicarb. Increased lint percentage was associated with application of aldicarb in 1994, but not in 1995. Fiber micronaire, elongation, uniformity, and strength were different among cultivars, but not between nematicide treatments. Fiber length (UHM; Upper-Half Mean) was different among cultivars and was higher in the aldicarb-treated plots in both years. Numbers of Meloidogyne incognita second stage juveniles and eggs extracted from soil samples were lower in the aldicarb-treated plots in July, but not at harvest, both years. Population densities of M. incognita were not different among cultivars, except the mid-season sample in 1995.


2017 ◽  
Vol 29 (1) ◽  
pp. 57
Author(s):  
Awais Murtza ◽  
Syed A. A. Bokhari ◽  
Iqra Kanwal ◽  
Yasir Ali ◽  
Tanvir Ahmad ◽  
...  

Fusarium wilt caused by F. oxysporum f.sp. capsici is a potential risk to successful production of chilli in Pakistan and causes huge yield loss. In present study, fifteen varieties/ lines were assessed against Fusarium wilt disease under Randomized Complete Block Design (RCBD) in the trial area of Department of Pla­nt Pathology, University of Agriculture Faisalabad for a long time amid 2012-2013 and 2013-2014. Results showed that none of variety showed highly resistant response towards disease. Gola peshawari showed resistant response with 5.1% infection frequency while six varieties i.e. Talhari (10.30), Harmal (13.26), Neelum (14.46), Burewala (20.13), Sanam (15.10) and FSD-1 (18.43) showed moderately resistant response. Four varieties i.e. FSD-2 (28.10), Ghotki (25.167%), KA-2 (23.133%), Tatapuri (V%), expressed moderately susceptible response with rating three and four varieties/lines i.e. Arunalu (40.33), NARC-4 (35.66), Sabazperi (32.13) and Skyline (30.50) expressed vulnerable response with score 4 to Fusarium wilt.


2020 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Mustaqim Mustaqim ◽  
Muhammad Fahrurrizal A. ◽  
Dasriani Dasriani ◽  
Hanifah Rahmawati ◽  
Putri Dhamira

Efforts in overcoming the problem of pathogenic mold attack can be done with the use of bacteria. This study aims to isolate the bacteria potentially controlling pathogenic molds in tomato plants (Solanum lycoperscium), as well as to identify potential bacteria controlling pathogenic fungi in tomato plants (Solanum lycoperscium) in Malang Regency. This research is an experimental laboratory study with a randomized block design (RCBD). This study used a Randomized Block Design because the experimental unit used was heterogeneous originating from 3 different locations. Of the 20 types of bacterial isolates, there are 6 bacteria that have the potential to inhibit the growth of Fusarium solani fungi that cause wilt disease in tomato plants, namely labeled bacteria EK, IK, BK, KK, CK and OK. There are six colonies that can act as antagonists of the fungus F. solani. It can be estimated that the six bacteria have the power to remodel and use carbon compounds. The carbon compounds such as arabinosa, xylosa, dextrose, cellobinosa, maltose, mannitol, sorbitol, and the use of citrate.


2013 ◽  
Vol 13 (2) ◽  
pp. 213
Author(s):  
Yasmín Socorro Cajas-Girón ◽  
Wilson Andrés Barragán Hernández ◽  
Wilson Andrés Barragán Hernández ◽  
Carlos Arreaza-Tavera ◽  
Carlos Arreaza-Tavera ◽  
...  

<p>Uno de los factores que limitan de manera importante la competitividad y sostenibilidad del sector ganadero de la región Caribe es la degradación de suelos y praderas. El objetivo del estudio fue evaluar en fincas del departamento del Atlántico y dedicadas a la de producción de carne, el efecto de las tecnologías de renovación de praderas como la intervención mecánica con labranza vertical, establecimiento de una nueva gramínea, aplicación de un plan de fertilización y pastoreo rotacional, sobre la producción de carne. Se seleccionaron tres fincas en las cuales se estableció una línea base en el año 2007 y posteriormente en los años 2008 y 2009 se evaluó el efecto de la renovación sobre la producción de forraje y carne. Se determinó la disponibilidad de forraje tanto en la pradera renovada como en la pradera sin renovación y cada mes se registró el peso de los animales. Los datos se analizaron empleando un diseño de bloques completos al azar con arreglo factorial. Los resultados mostraron un efecto significativo de la renovación de praderas en la producción de forraje, tanto en época seca como lluviosa (P &lt; 0.05). El efecto fue mayor en la época lluviosa que en la época seca (P &lt; 0.05). Las praderas renovadas tuvieron en promedio 1,5 veces más forraje que la pradera testigo (21,9 vs. 8,9 t de MS ha-1). Durante el período seco, la tecnología de renovación disminuyó el efecto negativo del estrés hídrico, manteniendo una producción promedio de forraje que duplicó la registrada durante el mismo período en la pradera sin intervención (5,2 vs. 2,5 t de MS ha-1). En la producción total de carne también se observó un efecto de renovación de praderas tanto en época seca como lluviosa. El efecto de la tecnología en producción de carne fue mayor en la época de lluvias que en la época seca (353 vs. 163 kg ha-1). El promedio general indicó que la producción de carne por unidad de área fue 4 veces mayor en la pradera renovada que en la pradera testigo (859 vs. 171 kg ha-1).Los resultados mostraron que la aplicación de tecnologías de renovación de praderas a nivel de finca en el departamento del Atlántico se logra aumentos significativos en producción de forraje y producción de carne por unidad de área lo cual tendría repercusiones en los ingresos de los productores. </p><p> </p><p><strong>Effect of the renewal technology pasture of <em>Bothriochloa pertusa </em>(L.) A. Camus on meat production in the North Coast of Colombia </strong></p><p>One of the factors that significantly limits the competitiveness and sustainability of the livestock sector in the Caribbean region is the degradation of soils and pastures. The objective of this study was to assess, on farms engaged in the production of meat in the Atlántico department, the effect of pasture renewal technologies, such as mechanical intervention with vertical tillage, establishment of a new grass, application of a fertilization plan and grazing rotation, on meat production.Three farms were selected in which a baseline was established in 2007 and subsequently, in 2008 and 2009, the effect of the renovation on the production of forage and meat was evaluated. Forage availability was determined in both the renewed and non-renewed pastures; and each month, the weight of the animals was recorded. The data were analyzed using a randomized block design with factorial arrangement. There was a significant effect of the applied technology on pasture production in both the wet season and the dry season (P &lt; 0.05). The effect was greater in the wet season than in the dry season (P &lt; 0.05). The recovered pasture had 1.5 times more forage in comparison with the control pasture (21.9 vs. 8.9 t DM ha-1). In the dry season, the applied technology decreased the detrimental effect of water stress, producing twice the amount of forage than the control pasture in the same period (5.2 vs. 2.5 t DM ha-1). The recovered pasture in the dry and wet seasons also positively affected total beef production. Meat production was higher in the rainy season than during the dry season (353 kg vs. 171 kg ha-1). The overall average indicated that meat production per unit area was 4 times higher in the recovered pasture than in the control pasture (859 kg vs. 171 kg ha-1). The results showed that pasture renewal technologies on farms in the Atlántico department provide significant improvements for forage and meat production per unit area that could have effects on the farmers incomes. </p>


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