scholarly journals Latest trends in L. infantum infection in dogs in Spain (Part II): current clinical management and control according to a national survey in practitioners

2020 ◽  
Author(s):  
Ana Montoya ◽  
Rosa Gálvez ◽  
Rocio Checa ◽  
Juliana Sarquis ◽  
Alexandra Plaza ◽  
...  
Keyword(s):  
Clinical Management ◽  
Complete Blood Count ◽  
Clinical Signs ◽  
Diagnostic Techniques ◽  
Control Measures ◽  
Canine Leishmaniosis ◽  
Cutaneous Lesions ◽  
Treatment Protocols ◽  
And Control ◽  
Monitoring Treatment

Abstract Background Canine leishmaniosis (CanL) is a parasitic zoonotic disease, endemic in the Mediterranean basin including Spain. While knowledge about CanL, its management, treatment, prevention and control mounts, it remains unclear whether all clinical veterinarians follow the same international recommendations, such as those of the LeishVet group. This study was thus designed to assess recent trends in the clinical management of CanL in veterinary clinics across Spain through a questionnaire-based survey. Results were compared with those of our prior national multicenter questionnaire administered by our research team in 2005. Methods A questionnaire consisting of 28 questions about CanL was developed using Google Forms and distributed by email to 1428 veterinary clinics in Spain. Questions were designed to obtain data on common clinical signs, techniques and complementary exams used to diagnose the disease, and on its monitoring, treatment and control measures. Data were collected in a database for statistical analysis. Results Completed questionnaires were returned by 295 clinics. Compared to the situation in 2005, replies indicate that clinical signs of CanL have not changed significantly, cutaneous lesions being still the most prevalent sign observed by practitioners. Quantitative serological techniques are considered an adequate approach to diagnosis, provided their results are supported by the findings of a thorough physical exam, as well as complementary tests (complete blood count (CBC), biochemical profile, plasma protein electrophoretogram and complete urinalysis). Treatment protocols and check-ups follow international recommendations. Finally, a multimodal approach is being endorsed to adequately control CanL including preventive measures such as: annual serological check-ups and the combination of repellents and vaccines. Besides, owners are being better informed about CanL by veterinarians, which translates to the improved control of this zoonosis. Conclusions The clinical management of CanL has recently undergone significant changes owing to improvements in clinical knowledge of the disease, more unified international criteria, improved diagnostic techniques and their adequate interpretation, as well as a greater awareness of the disease transmitted to owners.

scholarly journals An afebrile heartwater-like syndrome in goats

2013 ◽  
Vol 84 (1) ◽  
Author(s):  
Rhoda Leask ◽  
Kenneth P. Pettey ◽  
Gareth F. Bath
Keyword(s):  
Clinical Signs ◽  
Control Measures ◽  
Sub Saharan Africa ◽  
Limiting Factor ◽  
Febrile Reaction ◽  
Presumptive Diagnosis ◽  
Appropriate Treatment ◽  
Sub Saharan ◽  
Characteristic Features ◽  
And Control

Heartwater is a serious limiting factor for sheep and goat production in the major endemic area of sub-Saharan Africa and therefore most knowledge, research and control methods originate from this region. Whilst the usual or common clinical presentations can be used to make a presumptive diagnosis of heartwater with a good measure of confidence, this is not always the case, and animals suffering from heartwater may be misdiagnosed because their cases do not conform to the expected syndrome, signs and lesions. One aberrant form found occasionally in the Channel Island breeds of cattle and some goats is an afebrile heartwaterlike syndrome. The most constant and characteristic features of this heartwater-like syndrome comprise normal temperature, clinical signs associated with generalised oedema, and nervous signs, especially hypersensitivity. The presumption that the disease under investigation is the afebrile heartwater-like syndrome entails a tentative diagnosis based on history and clinical signs and the response to presumed appropriate treatment (metadiagnosis). The afebrile heartwater-like syndrome presents similarly to peracute heartwater but without the febrile reaction. Peracute cases of heartwater have a high mortality rate, enabling confirmation of the disease on post-mortem examination. Recognition of the afebrile heartwater-like syndrome is important to prevent deaths and identify the need for appropriate control measures.

scholarly journals The Diagnostic Challenges of Ovine Pulmonary Adenocarcinoma

Ruminants ◽  
2021 ◽  
Vol 1 (1) ◽  
pp. 58-71
Author(s):  
Hélder Quintas ◽  
Isabel Pires ◽  
Andreia Garcês ◽  
Justina Prada ◽  
Filipe Silva ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Pulmonary Adenocarcinoma ◽  
Diagnostic Methods ◽  
Control Measures ◽  
Economic Losses ◽  
Ovine Pulmonary Adenocarcinoma ◽  
X Ray ◽  
Club Cells ◽  
Pulmonary Adenomatosis ◽  
And Control

Ovine pulmonary adenocarcinoma (OPA), also known as sheep pulmonary adenomatosis and jaagsiekte, is a contagious pulmonary tumor of sheep, characterized by neoplastic proliferation of type II pneumocyte and club cells. OPA is induced by the oncogenic activity of the envelope glycoprotein (Env) of exogenous jaagsiekte sheep retrovirus (JSRV). This disease is associated with significant economic losses in numerous sheep raising countries. The onset of suggestive clinical signs is often late, making difficult the early diagnosis of the disease and timely implementation of control measures on the affected farms. Further, the lack of diagnostic tests that can be performed routinely by veterinary clinicians to accurately assess infected animals (e.g., serological or others) means that the true prevalence at flock level is not known. Imaging diagnostic methods (e.g., ultrasound, X-ray and computed tomography) can be used to support the clinical diagnosis, even in pre-clinical stages in affected flocks. The diagnosis must be confirmed by PCR of nasal excretions or immunohistochemistry and PCR of tumor lesions. No vaccine for OPA has yet been developed. Thus, in this work, we review the main methods of diagnosis of OPA in order to support the clinician in the identification of the disease, avoid underdiagnosis and allow the implementation of suitable measures to prevent and control its spread.

scholarly journals Genitourethral infections caused by D–K serotypes ofChlamydia trachomatis

2015 ◽  
Vol 156 (1) ◽  
pp. 19-23
Author(s):  
Péter Holló ◽  
Hajnalka Jókai ◽  
Krisztina Herszényi ◽  
Sarolta Kárpáti
Keyword(s):  
Chlamydia Trachomatis ◽  
Current Knowledge ◽  
Treatment Options ◽  
Clinical Signs ◽  
Diagnostic Techniques ◽  
Treatment Protocols ◽  
Sexually Transmitted ◽  
Screening Programs ◽  
Leading Role ◽  
Special Circumstances

Sexually transmitted infections of the urogenital tract are most commonly caused by the intracellular bacteria Chlamydia trachomatis worldwide, resulting the clinical picture of acute urethritis in men as well as urethritis and endocervicitis in women. As women often present with few symptoms only or a completely symptom-free disease course, one of the most important long-term complications is chronic pelvic inflammatory disease often followed by the development of infertility caused by chronic scar formation. Well-organized screening programs are considered to have a leading role in the prevention of disease spreading and long lasting unwanted complications. Antibiotic treatment options are often influenced by special circumstances, such as pregnancy and several complicated clinical forms. The aims of the authors are to give a concise review on the current knowledge regarding Chlamydia trachomatis infections and summarize typical clinical signs, modern diagnostic techniques as well as accepted treatment protocols and basic aspects of screening. Orv. Hetil., 2015, 156(1), 19–23.

scholarly journals Outbreak investigation and control case report of brucellosis: Experience from livestock research centre, Mpwapwa, Tanzania

2014 ◽  
Vol 81 (1) ◽  
Author(s):  
Gabriel M. Shirima ◽  
Seleman N. Masola ◽  
Obeid N. Malangu ◽  
Brant A. Schumaker
Keyword(s):  
Clinical Signs ◽  
Dairy Farms ◽  
Control Measures ◽  
Research Centre ◽  
Confirmatory Test ◽  
Medical Attention ◽  
Enzyme Linked Immunosorbent Assay ◽  
And Control ◽  
The Rose ◽  
S19 Vaccine

Brucellosis screening was conducted between 2005 and 2010 at the National Livestock Research Institute headquarters, Mpwapwa, Tanzania, following an abortion storm in cattle. The initial screening targeted breeding herds; 483 cattle were screened using the Rose Bengal Plate Test (RBPT) followed by the Competitive Enzyme-linked Immunosorbent Assay (c-ELISA) as a confirmatory test. The seropositivity on c-ELISA was 28.95% in 2005; it subsequently declined to 6.72%, 1.17%, 0.16% and 0.00% in 2006, 2007, 2009 and 2010, respectively. Brucella seropositivity was not detected in goats. Seropositivity declined following institution of stringent control measures that included: gradual culling of seropositive animals through slaughter; isolation and confinement of pregnant cows close to calving; proper disposal of placentas and aborted foetuses; the use of the S19 vaccine; and restricted introduction of new animals. It was thought that the source of this outbreak was likely to have been from the introduction of infected animals from another farm. Furthermore, humans were found with brucellosis antibodies. Out of 120 people screened, 12 (10%) were confirmed seropositive to brucella antigen exposure by c-ELISA analysis. The majority of the seropositive individuals (80%) were milkers and animal handlers from the farm. Nine individuals had clinical signs suggestive of brucellosis. All cases received medical attention from the district hospital. This achievement in livestock and human health showed that it is possible to control brucellosis in dairy farms, compared to pastoral and agro-pastoral farms, thus providing evidence to adopt these strategies in dairy farms thought to be at risk.

scholarly journals A U.S. isolate of Theileria orientalis, Ikeda genotype, is transmitted to cattle by the invasive Asian longhorned tick, Haemaphysalis longicornis

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Kelcey D. Dinkel ◽  
David R. Herndon ◽  
Susan M. Noh ◽  
Kevin K. Lahmers ◽  
S. Michelle Todd ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Clinical Signs ◽  
Adult Tick ◽  
Control Measures ◽  
Asia Pacific ◽  
Haemaphysalis Longicornis ◽  
Cattle Industry ◽  
Theileria Orientalis ◽  
And Control ◽  
The U.S

Abstract Background Theileria orientalis is a tick-borne hemoparasite that causes anemia, ill thrift, and death in cattle globally. The Ikeda strain of T.orientalis is more virulent than other strains, leading to severe clinical signs and death of up to 5% of affected animals. Within the Asia–Pacific region, where it affects 25% of Australian cattle, T.orientalis Ikeda has a significant economic impact on the cattle industry. In 2017, T.orientalis Ikeda was detected in a cattle herd in Albermarle County, Virginia, United States. Months earlier, the U.S. was alerted to the invasion of the Asian longhorned tick, Haemaphysalis longicornis, throughout the eastern U.S. Abundant H.longicornis ticks were identified on cattle in the T.orientalis-affected herd in VA, and a subset of ticks from the environment were PCR-positive for T.orientalis Ikeda. A strain of T.orientalis from a previous U.S. outbreak was not transmissible by H.longicornis; however, H.longicornis is the primary tick vector of T.orientalis Ikeda in other regions of the world. Thus, the objective of this study was to determine whether invasive H.longicornis ticks in the U.S. are competent vectors of T.orientalis Ikeda. Methods Nymphal H.longicornis ticks were fed on a splenectomized calf infected with the VA-U.S.-T.orientalis Ikeda strain. After molting, a subset of adult ticks from this cohort were dissected, and salivary glands assayed for T.orientalis Ikeda via qPCR. The remaining adult ticks from the group were allowed to feed on three calves. Calves were subsequently monitored for T.orientalis Ikeda infection via blood smear cytology and PCR. Results After acquisition feeding on a VA-U.S.-T.orientalis Ikeda-infected calf as nymphs, a subset of molted adult tick salivary glands tested positive by qPCR for T.orientalis Ikeda. Adult ticks from the same cohort successfully transmitted T.orientalis Ikeda to 3/3 naïve calves, each of which developed parasitemia reaching 0.4–0.9%. Conclusions Our findings demonstrate that U.S. H.longicornis ticks are competent vectors of the VA-U.S.-T.orientalis Ikeda strain. This data provides important information for the U.S. cattle industry regarding the potential spread of this parasite and the necessity of enhanced surveillance and control measures.

Salmonella spp. in pigs: an update on diagnostics and control

Livestock ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 38-43
Author(s):  
Christina Gale ◽  
Eduardo Velazquez
Keyword(s):  
Animal Health ◽  
Clinical Signs ◽  
Diagnostic Techniques ◽  
Antimicrobial Treatment ◽  
Diagnostics And Control ◽  
Salmonella Spp ◽  
Pig Farms ◽  
And Control ◽  
On Farm

Salmonella spp. is an important topic for both human and animal health, particularly Salmonella enterica ser. Typhimurium including its monophasic strains. Salmonella is an increasing problem on pig farms and the bacteria are transmitted through breeding pyramids, and can spread rapidly due to the high number of subclinical cases. Therefore, diagnosis on farm is extremely important, ensuring sampling from all areas of the unit to gain the overall infection picture. Modern diagnostic techniques allow differentiation between field and vaccine strains which is extremely important when implementing a vaccination programme on the farm. Vaccination accompanied by hygiene and biosecurity measures make up the three pillars of Salmonella spp. control, each of which should be given equal attention. Vaccination has been shown to be very effective in reducing environmental pressure and therefore prevalence and clinical signs due to S. enterica ser. Typhimurium. Vaccination forms part of successful Salmonella spp. control, although it is important to remember that Salmonella spp. control requires a long-term approach using a combination of interventions and avoiding the long-term use of antimicrobial treatment.

scholarly journals Autres orbivirus : Mise à jour des informations sur la peste équine africaine et la maladie hémorragique épizootique en Europe et dans le bassin méditerranéen

2009 ◽  
Vol 62 (2-4) ◽  
pp. 92
Author(s):  
M. Martinez ◽  
B. Rodriguez ◽  
J. M. Sanchez-Vizcaino
Keyword(s):  
Animal Health ◽  
Diagnostic Techniques ◽  
Social Consequences ◽  
Control Measures ◽  
Effective Vaccine ◽  
African Countries ◽  
Bassin Méditerranéen ◽  
Haemorrhagic Disease ◽  
Educational Campaigns ◽  
And Control

Orbiviruses are vector-borne pathogens that can cause notifi­able diseases in animals, such as bluetongue (BT) and epizootic haemorrhagic disease of deer (EHD) in ruminants, or African horse sickness (AHS) in equines. The relatively recent expansion of BT in Europe to higher latitudes than expected has evidenced the need to explore the ways of introduction and exposure of other orbiviruses in Europe and in the Mediterranean Basin. AHS was successfully eradicated from Europe since the 1990s but continues to be endemic in many African countries. Of the nine AHS serotypes, two have been present in Mediterranean coun­tries: AHS-9 (1966) and AHS-4 (1987-1990). The last outbreaks (up to 2008) of AHS in Africa classified by serotype occurred in Senegal (AHS-9), Kenya (AHS-4), and Nigeria, Senegal and Ethiopia (AHS-2). EHD is caused by 10 serotypes and is notifi­able to the World Organisation for Animal Health (OIE) since 2008. It is present in America, Australia, Asia and Africa and is known to affect wild ruminants as well as cattle. EHD has been present in cattle in North Africa (EHD-9) and the Middle East (EHD-7) since 2006. Transport of infected Culicoides from Northern Africa to Southern Europe by wind is a proved way of orbivirus introduction. Import of infected asymptomatic animals from an endemic country also happened the first time AHS was introduced in Spain. Then, certain environmental conditions such as warm temperatures can favour perpetuation of the dis­ease in animals exposed to infected vectors. The frequent con­sideration of horses as expensive leisure animals can worsen the economic and social consequences of a possible outbreak. However, nowadays there are good diagnostic techniques for AHS. Eradication can be achieved with the available polyvalent live vaccines and control measures. This is not the case for EHD, because an effective vaccine is urgently needed and there have been cross-reactions in the diagnoses between BT and EHD. European countries can prepare against other orbivirus outbreaks by prevention through educational campaigns and inactivated vaccine banks for AHS, and by further research on the possible vectors, the overwintering capacity of certain orbiviruses, the infectivity in all affected species, the identification of other pos­sible reservoirs, and the development of risk assessments and modelling.

scholarly journals A U.S. Isolate of Theileria Orientalis, Ikeda Genotype, is Transmitted to Cattle by the Invasive Asian Longhorned Tick, Haemaphysalis Longicornis

2020 ◽  
Author(s):  
Kelcey D Dinkel ◽  
David Herndon ◽  
Susan M Noh ◽  
Kevin K Lahmers ◽  
S. Michelle Todd ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Clinical Signs ◽  
Adult Tick ◽  
Control Measures ◽  
Asia Pacific ◽  
Haemaphysalis Longicornis ◽  
Cattle Industry ◽  
Theileria Orientalis ◽  
And Control ◽  
The U.S

Abstract BACKGROUNDTheileria orientalis is a tick-borne hemoparasite that causes anemia, ill thrift, and death in cattle globally. The Ikeda strain of T. orientalis is more virulent than other strains, leading to severe clinical signs and death in less than 5% of affected animals. Within the Asia-Pacific region, where it affects 25% of Australian cattle, T. orientalis Ikeda has a significant economic impact on the cattle industry. In 2017, T. orientalis Ikeda was detected in a cattle herd in Albermarle County, Virginia, United States. Months earlier, the U.S. was alerted to the invasion of the Asian longhorned tick, Haemaphysalis longicornis, throughout the eastern U.S. Abundant H. longicornis ticks were identified on cattle in the T. orientalis-affected herd in VA, and a subset of ticks from the environment were PCR-positive for T. orientalis Ikeda. A strain of T. orientalis from a previous U.S. outbreak was not transmissible by H. longicornis; however, H. longicornis is the primary tick vector of T. orientalis Ikeda in other regions of the world. Thus, the objective of this study was to determine whether invasive H. longicornis ticks in the U.S. are competent vectors of T. orientalis Ikeda. METHODSNymphal H. longicornis ticks were fed on a splenectomized calf infected with the VA-U.S.-T. orientalis Ikeda strain. After molting, a subset of adult ticks from this cohort were dissected, and salivary glands assayed for T. orientalis Ikeda via qPCR. The remaining adult ticks from the group were allowed to feed on three calves. Calves were subsequently monitored for T. orientalis Ikeda infection via blood smear cytology and PCR.RESULTSAfter acquisition feeding on a VA-U.S.-T. orientalis Ikeda-infected calf as nymphs, a subset of molted adult tick salivary glands tested positive by qPCR for T. orientalis Ikeda. Adult ticks from the same cohort successfully transmitted T. orientalis Ikeda to 3/3 naïve calves, each of which developed parasitemia reaching 0.4-0.9%.CONCLUSIONSOur findings demonstrate that U.S. H. longicornis ticks are competent vectors of the VA-U.S.-T. orientalis Ikeda strain. This data provides important information for the U.S. cattle industry regarding the potential spread of this parasite and the necessity of enhanced surveillance and control measures.

scholarly journals Early onset of clinical leishmaniosis in a litter of pups with evidence of in utero transmission

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Harold Salant ◽  
Yaarit Nachum‑Biala ◽  
Barbara Feinmesser ◽  
Maya Perelmutter ◽  
Gad Baneth
Keyword(s):  
Lymph Node ◽  
Vertical Transmission ◽  
Early Onset ◽  
Clinical Signs ◽  
Sand Fly ◽  
Enzyme Linked Immunosorbent Assay ◽  
Canine Leishmaniosis ◽  
Sand Flies ◽  
Cutaneous Lesions ◽  
Pcr Targeting

Abstract Background Canine leishmaniosis (CanL) is a zoonotic disease caused by Leishmania infantum. Although usually transmitted by phlebotomine sand flies, infection by vertical transmission and by blood transfusion have also been reported. Methods We describe the very early onset of clinical leishmaniosis, starting from 2 months of age, in a litter of pups born to an infected dam and sire. Seven pups from the litter of nine living in different households showed alopecic, exfoliative dermatitis and ulcerative cutaneous lesions. All pups and both parents were tested on at least one occasion both serologically, by enzyme-linked immunosorbent assay (ELISA), and by polymerase chain reaction (PCR) targeting the Leishmania ribosomal operon internal transcribed spacer 1 region and a short fragment of the kinetoplast minicircle; positive amplicons were sequenced. Results All nine pups were PCR positive for L. infantum verified by DNA sequencing, seven were positive by conjunctival, five by blood, four by lymph node, and one by skin PCR from an ulcerative lesion. Both pups with no clinical signs were seronegative, while five of the seven pups with dermatologic abnormalities were seropositive by ELISA. The sire had typical clinical dermatologic and visceral findings of CanL, was seropositive and PCR positive for L. infantum in the lymph node and fluid from the vas deferens tested after the testes were removed by castration. The dam was sub-clinically infected and seronegative, but positive by blood, lymph node and conjunctival PCR for L. infantum. Allopurinol administered to all clinically affected dogs resulted in clinical recovery. Conclusions Infection with L. infantum in both parents, the very early age of clinical onset among most of the pups, and the fact that the puppies were born and detected with signs of leishmaniosis in the winter, which is a season without sand fly activity in Israel, strongly suggest vertical transmission. Awareness of the possibility of vertical transmission of L. infantum and infection in littermates should be increased. It is recommended that littermates of young dogs with clinical leishmaniosis should be tested for sub-clinical infection as they may also be infectious to sand flies and thus to other dogs and to humans. Restricting the mating of infected bitches should also be considered to prevent the vertical transmission of the infection. Graphic abstract

scholarly journals Rabies Virus Infection in Livestock

2021 ◽  
Author(s):  
Abdelmalik I. Khalafalla ◽  
Yahia H. Ali
Keyword(s):  
Animal Health ◽  
Clinical Signs ◽  
Small Ruminants ◽  
Control Measures ◽  
Farm Animals ◽  
Modes Of Transmission ◽  
Dead End ◽  
Rabies Virus Infection ◽  
Prevention And Control Measures ◽  
And Control

Rabies is a lethal zoonotic encephalomyelitis and a major challenge to public and animal health. Livestock are affected by rabies mostly through bites of rapid dogs or wildlife carnivore\'s species. They are considered as ‘dead-end’ hosts that do not transmit the virus. Rabies in livestock has been endemic in many developing countries for many years and diagnosed through clinical signs and dog-biting history. An introduction on rabies situation in farm animals will be given then subchapters including `rabies in bovines, rabies in small ruminants, rabies in swine and rabies in camelids. In each subchapter we shall discuss, epidemiology, modes of transmission, diagnosis and prevention and control measures.

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