scholarly journals The Microbiota of a Mite Prey-predator System on Different Host Plants Are Characterized by Functional Redundancy and Dysbiosis

2021 ◽  
Author(s):  
Bruna Laís Merlin ◽  
Gilberto J Moraes ◽  
Fernando L Cônsoli
Keyword(s):  
Host Plant ◽  
Host Plants ◽  
Illumina Miseq ◽  
Predatory Mite ◽  
Life Cycles ◽  
Adult Females ◽  
Pinto Bean ◽  
Gene 16S Rrna ◽  
Miseq Platform ◽  
Predator System

Abstract Microbiota have diverse roles in the life cycles of their hosts, affecting their growth, development, behavior, and reproduction. Changes in physiological conditions of the host can also impact the assemblage of host-associated microorganisms. However, little is known of the effects of host plant–prey–predatory mite interactions on mite microbiota. We compared the microbial communities of eggs and adult females of the two˗spotted spider mite Tetranychus urticae Koch (Acari: Tetranychidae) and of adult females of the predatory mite Neoseiulus californicus (McGregor) (Acari: Phytoseiidae) on four different host plants (cotton, maize, pinto bean, and tomato) by metabarcoding sequencing of the V3–V4 region of the 16S ribosomal RNA gene (16S rRNA), using the Illumina MiSeq platform. Only the egg microbiota of T. urticae was affected by the host plant. The microbiota of the predatory mite N. californicus was very different from that of its prey, and the predator microbiota was unaffected by the different host plant–prey systems tested. Only the microbiota of the eggs of T. urticae carried Serratia as a high fidelity-biomarker. Biomarker bacteria were also detected in the microbiota of adult females of T. urticae and N. californicus, with different biomarkers in each host-plant species. The microbiota associated with eggs and adult females of T. urticae and adult females of N. californicus differed in their potential contributions to the host mite.

Influence of Dietary Lipids on Survival of Phyciodes phaon Butterflies (Lepidoptera: Nymphalidae)

2004 ◽  
Vol 39 (4) ◽  
pp. 537-544 ◽  
Author(s):  
Hanife Genc ◽  
James L. Nation
Keyword(s):  
Fatty Acids ◽  
Host Plant ◽  
Linolenic Acid ◽  
Artificial Diet ◽  
Dietary Lipids ◽  
Adult Survival ◽  
Plant Leaves ◽  
Adult Females ◽  
Pinto Bean ◽  
Freeze Dried

The Phaon crescent butterfly, Phyciodes phaon (Edwards), can be reared by placing newly-eclosed larvae on a pinto bean-based artificial diet; however, the adults fail to reproduce. Addition of 10% (w/w) of freeze-dried leaves of the host plant, Phyla nodiflora (L.) Greene, to the artificial diet approximately doubled the number of adults produced, and females oviposited viable eggs. Addition of wheat germ oil, linseed oil, or olive oil to the artificial diet without host plant tissue also increased survival to the adult stage, but adult females failed to oviposit. Larvae and adults reared from the artificial diet with addition of any one of the oils contained a higher quantity of linolenic acid in their body lipids than those reared on the artificial diet without additional oil. Leaves of the larval host plant are especially rich in the polyunsaturated fatty acids, linoleic acid and linolenic acid; whereas, the pinto bean diet is relatively low in these fatty acids, especially linolenic acid. Addition of synthetic β-sitosterol, stigmasterol, and campesterol, the sterols identified in the host plant leaves, to the artificial diet improved neither larval nor adult survival, and adult females did not oviposit. The factor (or factors) present in host plant leaves and acquired during larval feeding that enables reproduction of adults remains to be identified.

A TAXONOMIC MONOGRAPH OF THE NEARCTIC GALERUCINE GENUS OPHRAELLA WILCOX (COLEOPTERA: CHRYSOMELIDAE)

1986 ◽  
Vol 118 (S133) ◽  
pp. 3-75 ◽  
Author(s):  
Laurent LeSage
Keyword(s):  
United States ◽  
North America ◽  
Host Plant ◽  
Host Plants ◽  
The United States ◽  
Life Cycles ◽  
Ambrosia Artemisiifolia ◽  
Canadian Prairies ◽  
Young Leaves ◽  
Gulf States

AbstractThe taxonomy of the Nearctic genus Ophraella Wilcox is revised. Data for all known immature stages are included. Ophraella integra (LeConte) is synonymized with O. notulata (Fabricius). Ophraella dilatipennis (Jacoby) is transferred to the genus Neolochmaea Laboissière.Thirteen species are recognized, of which 6 are new : O. arctica, californiana, communa, macrovittata, nuda, and pilosa. The distribution and host plants of species are as follows : O. americana, eastern North America, on Solidago spp.; O. pilosa, transcontinental along the Canadian border, on Aster, primarily A. macrophyllus; O. cribrata, coast to coast in the United States, on Solidago of the subgenus Virgaurea; O. conferta in northeastern states, on Solidago with preference for S. canadensis and S. rugosa; O. sexvittata in southeastern states, on Solidago spp.; O. notulata in eastern US and Gulf states to Mexico, on Iva oraria; O. notata in eastern portion of the United States, on Eupatorium perfoliatum; O. macrovittata in the Gulf states, host plant unknown; O. communa in North America and Mexico, on Ambrosia artemisiifolia; O. bilineata in the Canadian Prairies and the bordering states, on Chrysopsis villosa; O. californiana in California and Mexico, on Artemisia Douglasiana; O. nuda in Alberta, host plant unknown; and O. arctica in tundra zone, on Solidago multiradiata scopulorum.The life cycles of most species are still unknown but most species probably have only 1 generation per year. The eggs are laid in clusters on the under surface of young leaves. The larvae skeletonize young leaves and live exposed. Before pupation, the larva spins a loose cocoon and attaches it to a leaf tip. Pupation lasts 1–2 weeks. The newly hatched adults are active on host plants until the early fall, when they enter the leaf litter for overwintering.

scholarly journals PSIX-6 Gastrointestinal microbiome of calves fed solid diets containing different levels and sources of NDF

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 418-419
Author(s):  
Gercino F Virgínio Júnior ◽  
Milaine Poczynek ◽  
Ana Paula Silva ◽  
Ariany Toledo ◽  
Amanda Cezar ◽  
...  
Keyword(s):  
Illumina Miseq ◽  
Diversity Indices ◽  
Dairy Calves ◽  
Rrna Gene ◽  
Gastrointestinal Microbiome ◽  
Fecal Microbiome ◽  
Miseq Platform ◽  
Holstein Calves ◽  
Ad Libitum ◽  
Different Levels

Abstract Different levels and sources of NDF can modify the gastrointestinal microbiome. This study evaluated 18 Holstein calves housed in not-bedded suspended individual cages and fed one of three treatments: 22NDF - conventional starter containing 22% NDF (n = 7); 31NDF - starter with 31% NDF, replacing part of the corn by soybean hull (n = 6); and 22NDF+H - conventional starter with 22% NDF plus coast-cross hay ad libitum (n = 5). All animals received 4 L of milk replacer daily (24% CP; 18.5% fat; diluted to 12.5% solids), divided into two meals, being weaned at 8th week of age. After weaning, animals were housed in tropical shelters, fed with the respective solid diet and coast-cross hay ad libitum for all treatments. To evaluate the microbiome, ruminal fluid samples were collected using a modified Geishauser oral probe at weeks 2, 4, 6, 8 and 10, two hours after the morning feeding, and fecal samples were collected at birth (0) and at weeks 1, 2, 4, 8 and 10. The microbial community was determined by sequencing V3 and V4 region amplicons of the 16S rRNA gene that was amplified by PCR and sequenced by the Illumina MiSeq platform. Ruminal microbiome had no differences in diversity for the effects of weeks, treatments or interaction of both factors (Table 1). In feces, the diversity indices and evenness were higher for 22NDF+H when compared to 22NDF, with no difference for 31NDF. All indices were significantly affected by calves age. At birth, calves had the greatest diversity and richness. Week 1 and 2 had less evenness and diversity. Bacteroidota, Firmicutes_A and Firmicutes_C were the most abundant phylum in rumen and feces. The supply of hay was only effective in modifying the fecal microbiome of dairy calves, suggesting a resilience in the ruminal microbiome.

scholarly journals Effect of host plant on the life history of the carnation tortrix moth Cacoecimorpha pronubana (Lepidoptera: Tortricidae)

2021 ◽  
pp. 1-7
Author(s):  
Marcin W. Zielonka ◽  
Tom W. Pope ◽  
Simon R. Leather
Keyword(s):  
Life History ◽  
Host Plant ◽  
Plant Species ◽  
Host Plants ◽  
Crop Protection ◽  
Plant Production ◽  
Pupal Weight ◽  
Production Environment ◽  
Host Plant Species ◽  
The Uk

Abstract The carnation tortrix moth, Cacoecimorpha pronubana (Hübner, [1799]) (Lepidoptera: Tortricidae), is one of the most economically important insect species affecting the horticultural industry in the UK. The larvae consume foliage, flowers or fruits, and/or rolls leaves together with silken threads, negatively affecting the growth and/or aesthetics of the crop. In order to understand the polyphagous behaviour of this species within an ornamental crop habitat, we hypothesized that different host plant species affect its life history traits differently. This study investigated the effects of the host plant species on larval and pupal durations and sizes, and fecundity (the number of eggs and the number and size of egg clutches). At 20°C, 60% RH and a 16L:8D photoperiod larvae developed 10, 14, 20 and 36 days faster when reared on Christmas berry, Photinia (Rosaceae), than on cherry laurel, Prunus laurocerasus (Rosaceae), New Zealand broadleaf, Griselinia littoralis (Griseliniaceae), Mexican orange, Choisya ternata (Rutaceae), and firethorn, Pyracantha angustifolia (Rosaceae), respectively. Female pupae were 23.8 mg heavier than male pupae, and pupal weight was significantly correlated with the duration of larval development. The lowest and the highest mean numbers of eggs were produced by females reared on Pyracantha (41) and Photinia (202), respectively. Clutch size differed significantly among moths reared on different host plants, although the total number of eggs did not differ. This study showed that different ornamental host plants affect the development of C. pronubana differently. Improved understanding of the influence of host plant on the moth's life history parameters measured here will help in determining the economic impact that this species may have within the ornamental plant production environment, and may be used in developing more accurate crop protection methodologies within integrated pest management of this insect.

scholarly journals Root-associated entomopathogenic fungi manipulate host plants to attract herbivorous insects

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Belén Cotes ◽  
Gunda Thöming ◽  
Carol V. Amaya-Gómez ◽  
Ondřej Novák ◽  
Christian Nansen
Keyword(s):  
Host Plant ◽  
Entomopathogenic Fungi ◽  
Host Plants ◽  
Near Infrared ◽  
Delia Radicum ◽  
Host Plant Selection ◽  
Herbivorous Insects ◽  
Herbivorous Insect ◽  
Insect Performance ◽  
Leaf Reflectance

AbstractRoot-associated entomopathogenic fungi (R-AEF) indirectly influence herbivorous insect performance. However, host plant-R-AEF interactions and R-AEF as biological control agents have been studied independently and without much attention to the potential synergy between these functional traits. In this study, we evaluated behavioral responses of cabbage root flies [Delia radicum L. (Diptera: Anthomyiidae)] to a host plant (white cabbage cabbage Brassica oleracea var. capitata f. alba cv. Castello L.) with and without the R-AEF Metarhizium brunneum (Petch). We performed experiments on leaf reflectance, phytohormonal composition and host plant location behavior (behavioral processes that contribute to locating and selecting an adequate host plant in the environment). Compared to control host plants, R-AEF inoculation caused, on one hand, a decrease in reflectance of host plant leaves in the near-infrared portion of the radiometric spectrum and, on the other, an increase in the production of jasmonic, (+)-7-iso-jasmonoyl-l-isoleucine and salicylic acid in certain parts of the host plant. Under both greenhouse and field settings, landing and oviposition by cabbage root fly females were positively affected by R-AEF inoculation of host plants. The fungal-induced change in leaf reflectance may have altered visual cues used by the cabbage root flies in their host plant selection. This is the first study providing evidence for the hypothesis that R-AEF manipulate the suitability of their host plant to attract herbivorous insects.

scholarly journals A Rapid Bacteriophage DNA Extraction Method

2018 ◽  
Vol 1 (3) ◽  
pp. 27 ◽  
Author(s):  
Džiuginta Jakočiūnė ◽  
Arshnee Moodley
Keyword(s):  
Dna Extraction ◽  
Illumina Miseq ◽  
Extraction Methods ◽  
Resistant Bacteria ◽  
Simple Method ◽  
Antibiotic Resistant ◽  
Phage Dna ◽  
Specialized Equipment ◽  
Miseq Platform ◽  
Dna Extraction Methods

Bacteriophages (phages) are intensely investigated as non-antibiotic alternatives to circumvent antibiotic resistance development as well as last resort therapeutic options against antibiotic resistant bacteria. As part of gaining a better understanding of phages and to determine if phages harbor putative virulence factors, whole genome sequencing is used, for which good quality phage DNA is needed. Traditional phage DNA extraction methods are tedious and time consuming, requiring specialized equipment e.g., an ultra-centrifuge. Here, we describe a quick and simple method (under four hours) to extract DNA from double stranded DNA (dsDNA) phages at titers above 1.0 × 1010 plaque-forming units (PFU)/mL. This DNA was suitable for library preparation using the Nextera XT kit and sequencing on the Illumina MiSeq platform.

The ability of rhizosphere bacteria isolated from nematode host and non-host plants to influence the hatch in vitro of the two potato cyst nematode species, Globodera rostochiensis and G. pallida

Nematology ◽  
2004 ◽  
Vol 6 (3) ◽  
pp. 375-387 ◽  
Author(s):  
N. Aileen Ryan ◽  
Peter Jones
Keyword(s):  
Sugar Beet ◽  
Host Plant ◽  
Host Plants ◽  
Nematode Species ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Globodera Pallida ◽  
Bacterial Isolates ◽  
Bacillus Spp

AbstractSeventy bacteria, isolated from the rhizosphere of the potato cyst nematode (PCN) host plant, potato, were cultured in the presence and absence of potato root leachate (PRL) and the resultant culture filtrates were analysed for their ability to affect the hatch in vitro of the two PCN species. Of the isolates tested, nine had a significant effect on PCN hatch. Six affected Globodera pallida hatch and three affected G. rostochiensis hatch. Five of the isolates significantly increased hatch only when cultured in the presence of PRL. Three of the isolates decreased PCN hatch significantly in PRL. Only one isolate increased hatch significantly in the absence of PRL. No isolate affected the hatch of both species. Six of the nine isolates that significantly affected PCN hatch had been pre-selected by culturing on PRL. Bacterial isolates from PCN non-hosts (14 from wheat, 17 from sugar beet) were also tested for hatching activity. The principal effect of the hatch-active isolates from the PCN non-host plants was to increase PCN hatch in the presence of PRL. In contrast to the host bacteria results, the isolates from non-host plants affected only G. rostochiensis hatch (three wheat isolates and four sugar beet isolates significantly increased G. rostochiensis hatch); no such isolate affected G. pallida hatch significantly in the presence of PRL. Ten isolates (32%) from non-host plants had the ability to increase significantly the hatch of PCN in the absence of PRL (eight of these affected G. rostochiensis hatch and four affected G. pallida hatch), compared to only one bacterial isolate (1%) from a host plant. The majority of the isolates from non-hosts produced PCN species-specific effects, as with the bacteria isolated from potatoes, although two wheat isolates increased the hatch of both species significantly in the absence of PRL. Of 20 hatch-active bacterial isolates (from all three plants) identified, 70% were Bacillus spp. Other genera identified were Arthrobacter , Acinetobacter and Staphylococcus .

Effects of short-term heat stress on the development and reproduction of predatory mite Neoseiulus barkeri (Acari, Phytoseiidae)

2021 ◽  
Vol 26 (4) ◽  
pp. 713-723
Author(s):  
Wei Zhen Li ◽  
Hao Long Li ◽  
Zi Kun Guo ◽  
Su Qin Shang
Keyword(s):  
Heat Stress ◽  
Life Span ◽  
Treatment Time ◽  
Predatory Mite ◽  
Immature Stage ◽  
Oviposition Period ◽  
Hatching Rate ◽  
Short Term ◽  
Developmental Duration ◽  
Adult Females

The predatory mite Neoseiulus barkeri (Acari: Phytoseiidae), which is one of the best natural enemies, has been used as a biological control agent against multiple insect pests, such as spider mite, Tetranychus urticae and thrips. Its growth and development were affected by the environmental temperature changes. This study was conducted to evaluate the hatching rate of the eggs and the immature developmental times of N. barkeri after incubation of the eggs under heat stress at 38, 40 and 42℃, 85% ± 5% RH and a 16h:8h light: dark (L:D) photoperiod for 2, 4 and 6h. After adult females emerged, they were treated under the same conditions again, and the parameters such as oviposition period, fecundity and female longevity were observed. The results showed that with the increase of the temperature and the extension of the duration of the heat stress, the hatching rate was lower. The eggs treated at 42℃ for 2h or more could not hatch, and the developmental duration of each immature stage showed a trend of decreasing at first and then increasing. The fastest development was observed after incubation of eggs at 40℃ for 2h, and the shortest developmental duration was 4.60d. Under the condition of 38℃, the oviposition period and life span of adult females were shortened with the extension of treatment time, and the fecundity decreased at first and then increased. At 40℃, the fecundity, oviposition period and longevity of adult females showed a trend of gradual increase with the extension of treatment time. The shortest oviposition period, the lowest fecundity and the shortest life span of adult females were 12.14d, 18.92 eggs and 16.65d, respectively after incubation of eggs at 40℃, for 2h. Therefore, the short-term heat stress had a significant effect on the hatching rate and the duration of the immature stage, and also had a negative effect on the fecundity, oviposition period and longevity of adult females. In rearing and field release, heat stress should be avoided as much as possible to achieve the best predation and control effect of N. barkeri.

scholarly journals A novel proof of concept for capturing the diversity of endophytic fungi preserved in herbarium specimens

2018 ◽  
Vol 374 (1763) ◽  
pp. 20170395 ◽  
Author(s):  
Barnabas H. Daru ◽  
Elizabeth A. Bowman ◽  
Donald H. Pfister ◽  
A. Elizabeth Arnold
Keyword(s):  
Species Interactions ◽  
Fungal Endophytes ◽  
Illumina Miseq ◽  
Amplicon Sequencing ◽  
Herbarium Specimens ◽  
Proof Of Concept ◽  
Ledum Palustre ◽  
Surface Contaminants ◽  
Symptomless Plant ◽  
Miseq Platform

Herbarium specimens represent important records of morphological and genetic diversity of plants that inform questions relevant to global change, including species distributions, phenology and functional traits. It is increasingly appreciated that plant microbiomes can influence these aspects of plant biology, but little is known regarding the historic distribution of microbes associated with plants collected in the pre-molecular age. If microbiomes can be observed reliably in herbarium specimens, researchers will gain a new lens with which to examine microbial ecology, evolution, species interactions. Here, we describe a method for accessing historical plant microbiomes from preserved herbarium specimens, providing a proof of concept using two plant taxa from the imperiled boreal biome ( Andromeda polifolia and Ledum palustre subsp . groenlandicum, Ericaceae). We focus on fungal endophytes, which occur within symptomless plant tissues such as leaves. Through a three-part approach (i.e. culturing, cloning and next-generation amplicon sequencing via the Illumina MiSeq platform, with extensive controls), we examined endophyte communities in dried, pressed leaves that had been processed as regular herbarium specimens and stored at room temperature in a herbarium for four years . We retrieved only one endophyte in culture, but cloning and especially the MiSeq analysis revealed a rich community of foliar endophytes. The phylogenetic distribution and diversity of endophyte assemblages, especially among the Ascomycota, resemble endophyte communities from fresh plants collected in the boreal biome. We could distinguish communities of endophytes in each plant species and differentiate likely endophytes from fungi that could be surface contaminants. Taxa found by cloning were observed in the larger MiSeq dataset, but species richness was greater when subsets of the same tissues were evaluated with the MiSeq approach. Our findings provide a proof of concept for capturing endophyte DNA from herbarium specimens, supporting the importance of herbarium records as roadmaps for understanding the dynamics of plant-associated microbial biodiversity in the Anthropocene. This article is part of the theme issue ‘Biological collections for understanding biodiversity in the Anthropocene’.

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