Plasmon-Activated Water Decreases Vasculopathy in Orthotopic Allograft Transplantation Rats

Abstract Patients undergoing orthotopic allograft transplantation (OAT) will certainly suffer from vasculopathy. Although there are many immunosuppressive and immunomodulatory agents that are administered to patients, chronic rejection- induced vasculopathy cannot be entirely managed. Moreover, the implanted graft might become dysfunctional. In the past, we have used deionized reverse osmosis water (ROW) to stream via gold nanoparticles (AuNPs) at room temperature under powerful illumination, in order to prepare plasmon-activated water (PAW) with fewer hydrogen bonds. Compared to ROW, stable PAW can successfully remove free hydroxyl and 2,2-diphenyl-1-picrylhydrazyl radicals, and efficiently reduce lipopolysaccharide (LPS)-induced monocytes to release nitric oxide. Moreover, PAW can considerably induce the expression of the antioxidant gene Nrf2 in human gingival fibroblasts. Moreover, it might lower amyloid burden in mice with Alzheimer's disease. Furthermore, PAW decreased metastasis in mice grafted with Lewis lung carcinoma cells and boosted the overall survival in combination with cisplatin. Because of this possibility that PAW could prevent systemic disease, we aimed to evaluate the influence of PAW on OAT-induced vasculopathy. Here, we demonstrated that daily intake of PAW lowered the progression of vasculopathy in OAT-recipient ACI/NKyo rats by inhibiting collagen accumulation, proliferation of smooth muscle cells and fibroblasts, and T lymphocyte infiltration in the vessel wall. Moreover, the results showed reduced T and B lymphocytes, plasma cells, and macrophage activation in the spleen of the OAT-recipient ACI/NKyo rats that were administered PAW. Finally, in contrast to the control group, the OAT-recipient ACI/NKyo rats that were administered PAW exhibited higher mobilization and levels of circulating endothelial progenitor cells associated with vessel repair. Therefore, this study highlights the therapeutic roles of PAW in vasculopathy.

Download Full-text

Transferring Plasmon Effect on a Biological System: Expression of Biological Polymers in Chronic Rejection and Inflammatory Rat Model

Polymers ◽

10.3390/polym13111827 ◽

2021 ◽

Vol 13 (11) ◽

pp. 1827

Author(s):

Chien-Sung Tsai ◽

Feng-Yen Lin ◽

Yu-Chuan Liu ◽

Yi-Wen Lin ◽

Yi-Ting Tsai ◽

...

Keyword(s):

Hydrogen Bonds ◽

Biological System ◽

Plasma Cells ◽

Physiological Activity ◽

Daily Intake ◽

Biochemical Properties ◽

Control Group ◽

Lung Carcinoma Cells ◽

Circulating Endothelial Progenitor Cells ◽

Plasmon Effect

The plasmon-activated water (PAW) that reduces hydrogen bonds is made of deionized reverse osmosis water (ROW). However, compared with ROW, PAW has a significantly higher diffusion coefficient and electron transfer rate constant in electrochemical reactions. PAW has a boiling point of97 °C and specific heat of0.94; the energy of PAW is also 1121 J/mol higher than ordinary water. The greater the force of hydrogen bonds between H2O, the larger the volume of the H2O cluster, and the easier it is to lose the original characteristics. The hydrogen bonding force of PAW is weak, so the volume of its cluster is small, and it exists in a state very close to a single H2O. PAW has a high permeability and diffusion rate, which can improve the needs of biological applications and meet the dependence of biological organisms on H2O when performing physiological functions. PAW can successfully remove free radicals, and efficiently reduce lipopolysaccharide (LPS)-induced monocytes to release nitric oxide. PAW can induce expression of the antioxidant gene Nrf2 in human gingival fibroblasts, lower amyloid burden in mice with Alzheimer’s disease, and decrease metastasis in mice grafted with Lewis lung carcinoma cells. Because the transferring plasmon effect may improve the abnormality of physiological activity in a biological system, we aimed to evaluate the influence of PAW on orthotopic allograft transplantation (OAT)-induced vasculopathy in this study. Here, we demonstrated that daily intake of PAW lowered the progression of vasculopathy in OAT-recipient ACI/NKyo rats by inhibiting collagen accumulation, proliferation of smooth muscle cells and fibroblasts, and T lymphocyte infiltration in the vessel wall. The results showed reduced T and B lymphocytes, plasma cells, and macrophage activation in the spleen of the OAT-recipient ACI/NKyo rats that were administered PAW. In contrast to the control group, the OAT-recipient ACI/NKyo rats that were administered PAW exhibited higher mobilization and levels of circulating endothelial progenitor cells associated with vessel repair. We use the transferring plasmon effect to adjust and maintain the biochemical properties of water, and to meet the biochemical demand of organisms. Therefore, this study highlights the therapeutic roles of PAW and provides more biomedical applicability for the transferring plasmon effect.

Download Full-text

Ruxolitinib Regulates the Autophagy Machinery in Multiple Myeloma Cells

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520620666200218105159 ◽

2020 ◽

Vol 20 (18) ◽

pp. 2316-2323 ◽

Cited By ~ 3

Author(s):

Alican Kusoglu ◽

Bakiye G. Bagca ◽

Neslihan P.O. Ay ◽

Guray Saydam ◽

Cigir B. Avci

Keyword(s):

Multiple Myeloma ◽

Cell Line ◽

B Lymphocyte ◽

Plasma Cells ◽

Annexin V ◽

Myeloma Cell ◽

Control Group ◽

Multiple Myeloma Cell ◽

Ic50 Values ◽

Stat Pathway

Background: Ruxolitinib is a selective JAK1/2 inhibitor approved by the FDA for myelofibrosis in 2014 and nowadays, comprehensive investigations on the potential of the agent as a targeted therapy for haematological malignancies are on the rise. In multiple myeloma which is a cancer of plasma cells, the Interleukin- 6/JAK/STAT pathway is emerging as a therapeutic target since the overactivation of the pathway is associated with poor prognosis. Objective: In this study, our purpose was to discover the potential anticancer effects of ruxolitinib in ARH-77 multiple myeloma cell line compared to NCI-BL 2171 human healthy B lymphocyte cell line. Methods: Cytotoxic effects of ruxolitinib in ARH-77 and NCI-BL 2171 cells were determined via WST-1 assay. The autophagy mechanism induced by ruxolitinib measured by detecting autophagosome formation was investigated. Apoptotic effects of ruxolitinib were analyzed with Annexin V-FITC Detection Kit and flow cytometry. We performed RT-qPCR to demonstrate the expression changes of the genes in the IL-6/JAK/STAT pathway in ARH-77 and NCI-BL 2171 cells treated with ruxolitinib. Results: We identified the IC50 values of ruxolitinib for ARH-77 and NCI-BL 2171 as 20.03 and 33.9μM at the 72nd hour, respectively. We showed that ruxolitinib induced autophagosome accumulation by 3.45 and 1.70 folds in ARH-77 and NCI-BL 2171 cells compared to the control group, respectively. Treatment with ruxolitinib decreased the expressions of IL-6, IL-18, JAK2, TYK2, and AKT genes, which play significant roles in MM pathogenesis. Conclusion: All in all, ruxolitinib is a promising agent for the regulation of the IL-6/JAK/STAT pathway and interferes with the autophagy mechanism in MM.

Download Full-text

Epidemiology, Risk Factors, and Outcomes of Opportunistic Infections after Kidney Allograft Transplantation in the Era of Modern Immunosuppression: A Monocentric Cohort Study

Journal of Clinical Medicine ◽

10.3390/jcm8050594 ◽

2019 ◽

Vol 8 (5) ◽

pp. 594 ◽

Cited By ~ 4

Author(s):

Philippe Attias ◽

Giovanna Melica ◽

David Boutboul ◽

Nathalie De Castro ◽

Vincent Audard ◽

...

Keyword(s):

Risk Factors ◽

Protective Factor ◽

Opportunistic Infections ◽

Control Group ◽

Kidney Allograft ◽

Extended Criteria Donor ◽

Allograft Transplantation ◽

Kidney Recipients ◽

Independent Risk Factors ◽

Kidney Transplantations

Epidemiology of opportunistic infections (OI) after kidney allograft transplantation in the modern era of immunosuppression and the use of OI prevention strategies are poorly described. We retrospectively analyzed a single-center cohort on kidney allograft adult recipients transplanted between January 2008 and December 2013. The control group included all kidney recipients transplanted in the same period, but with no OI. We analyzed 538 kidney transplantations (538 patients). The proportion of OI was 15% (80 and 72 patients). OI occurred 12.8 (6.0–31.2) months after transplantation. Viruses were the leading cause (n = 54, (10%)), followed by fungal (n = 15 (3%)), parasitic (n = 6 (1%)), and bacterial (n = 5 (0.9%)) infections. Independent risk factors for OI were extended criteria donor (2.53 (1.48–4.31), p = 0.0007) and BK viremia (6.38 (3.62–11.23), p < 0.0001). High blood lymphocyte count at the time of transplantation was an independent protective factor (0.60 (0.38–0.94), p = 0.026). OI was an independent risk factor for allograft loss (2.53 (1.29–4.95), p = 0.007) but not for patient survival. Post-kidney transplantation OIs were mostly viral and occurred beyond one year after transplantation. Pre-transplantation lymphopenia and extended criteria donor are independent risk factors for OI, unlike induction therapy, hence the need to adjust immunosuppressive regimens to such transplant candidates.

Download Full-text

Clinical and Immunological Efficacy of Mangosteen and Propolis Extracted Complex in Patients with Gingivitis: A Multi-Centered Randomized Controlled Clinical Trial

Nutrients ◽

10.3390/nu13082604 ◽

2021 ◽

Vol 13 (8) ◽

pp. 2604

Author(s):

Jin-Young Park ◽

Kyung-A Ko ◽

Ji-Yeong Lee ◽

Jae-Woon Oh ◽

Hyun-Chang Lim ◽

...

Keyword(s):

Patient Reported Outcomes ◽

Daily Intake ◽

Test Group ◽

Control Group ◽

Controlled Clinical Trial ◽

Control Groups ◽

Immunological Parameters ◽

Significant Difference ◽

Patient Reported ◽

And Control

Background: Mangosteen and propolis extracts (MAEC) have been potential therapeutic agents known to exhibit powerful antioxidant and anti-inflammatory properties. The aim of the current study was to evaluate the clinical and immunological efficacy of MAEC as well as safety and patient-reported outcomes (PROMs) on gingivitis and incipient periodontitis. Methods: This study was performed on 104 patients diagnosed with gingivitis or incipient periodontitis. At baseline, the participants were randomly allocated to either the test group, with daily intake of a single capsule containing 194 mg of MAEC for eight weeks, or control group, with placebo. Clinical periodontal evaluation and immunological parameters from saliva and gingival sulcular fluid were assessed at baseline, four, and eight weeks. Individual PROMs were assessed by OHIP-14 questionnaires. Results: There was a significant difference of modified gingival index at four and eight weeks between the test and control groups. In the test group, crevicular interleukin (IL)-6 was reduced, and the salivary matrix metalloproteinase (MMP)-9 was increased after eight weeks. PROMs were improved up to four weeks compared to placebo. Conclusion: Oral administration of MAEC would have a potential to reduce gingival inflammation clinically and immunologically in the patients with gingivitis and incipient periodontitis.

Download Full-text

THE EPIDEMIOLOGY OF PEDIATRIC MENISCAL ALLOGRAFT TRANSPLANTATION IN THE UNITED STATES

Orthopaedic Journal of Sports Medicine ◽

10.1177/2325967121s00040 ◽

2021 ◽

Vol 9 (7_suppl3) ◽

pp. 2325967121S0004

Author(s):

Haley E. Smith ◽

Madeline M. Lyons ◽

Neeraj M. Patel

Keyword(s):

United States ◽

Pediatric Population ◽

The United States ◽

National Database ◽

Control Group ◽

Meniscal Allograft Transplantation ◽

Children's Hospitals ◽

Allograft Transplantation ◽

Meniscal Allograft ◽

Children’S Hospitals

Background: Meniscal allograft transplantation (MAT) is an option to slow the progression of degenerative disease in the setting of substantial meniscal deficiency. This may be especially important in children and adolescents, but there is little literature on MAT in this population. Hypothesis/Purpose: The purpose of this study was to evaluate the epidemiology of MAT in the pediatric population, with specific attention to regional and demographic trends. Methods: The Pediatric Health Information System, a national database consisting of 49 children’s hospitals, was queried for all patients undergoing MAT between 2011 and 2018. Demographic information was collected for each subject as well as data regarding previous and subsequent surgeries. The database was also queried for all meniscus surgeries (including repairs and meniscectomies) performed during the study period. Demographic and geographic data from this control group were compared to that of children undergoing MAT. Univariate analysis was followed by purposeful entry multivariate regression to adjust for confounding factors. Results: A total of 27,168 meniscus surgeries were performed in 47 hospitals, with MAT performed 67 times in 17 hospitals. Twelve patients (18%) underwent a subsequent procedure after transplantation. In multivariate analysis, each year of increasing age resulted in 1.1 times higher odds of having undergone MAT rather than repair or meniscectomy (95% CI 1.03-1.1, p=0.002). Patients that underwent transplantation had 2.0 times higher odds of being female (95% CI 1.2-3.3, p=0.01) and 2.0 times higher odds of being commercially insured (95% CI 1.1-3.6, p=0.02). MAT was performed most frequently in the Northeast (4.9/1000 meniscus surgeries) and least often in the South (1.1/1000 meniscus surgeries, p<0.001). Furthermore, transplantation was more likely to be performed in larger cities. The median pediatric population of cities in which MAT was performed was 983,268 (range 157,253-3,138,870) compared to 662,290 (range 4,420-4,311,500) in cities where it was not (p=0.04). Conclusion: In the United States, patients that underwent MAT were older, more likely to be female, and have commercial insurance than those undergoing meniscus repair or meniscectomy. MAT was only done in 17/47 children’s hospitals that perform meniscus surgery and was most frequently performed in the Northeast and in larger cities. These trends highlight the need for further research, especially regarding differences along the lines of sex and insurance status.

Download Full-text

Magneto-reactance based detection of MnO nanoparticle-embedded Lewis lung carcinoma cells

Journal of Applied Physics ◽

10.1063/1.4914950 ◽

2015 ◽

Vol 117 (17) ◽

pp. 17D123 ◽

Cited By ~ 9

Author(s):

J. Devkota ◽

M. Howell ◽

P. Mukherjee ◽

H. Srikanth ◽

S. Mohapatra ◽

...

Keyword(s):

Lung Carcinoma ◽

Lewis Lung Carcinoma ◽

Carcinoma Cells ◽

Lewis Lung ◽

Lung Carcinoma Cells ◽

Lewis Lung Carcinoma Cells

Download Full-text

Phyllanthus urinaria triggers the apoptosis and Bcl-2 down-regulation in Lewis lung carcinoma cells

Life Sciences ◽

10.1016/s0024-3205(03)00016-x ◽

2003 ◽

Vol 72 (15) ◽

pp. 1705-1716 ◽

Cited By ~ 55

Author(s):

Sheng-Teng Huang ◽

Rong-Chi Yang ◽

Li-Jiun Yang ◽

Pei-Nir Lee ◽

Jong-Hwei S. Pang

Keyword(s):

Lung Carcinoma ◽

Lewis Lung Carcinoma ◽

Carcinoma Cells ◽

Lewis Lung ◽

Down Regulation ◽

Lung Carcinoma Cells ◽

Phyllanthus Urinaria ◽

Lewis Lung Carcinoma Cells

Download Full-text

Expression of HIF-2αand VEGF in Cervical Squamous Cell Carcinoma and Its Clinical Significance

BioMed Research International ◽

10.1155/2016/5631935 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Lixia Zhang ◽

Qiang Chen ◽

Jing Hu ◽

Yue Chen ◽

Chenglong Liu ◽

...

Keyword(s):

Lymph Node ◽

Lymph Node Metastasis ◽

Clinical Significance ◽

Systemic Disease ◽

Cervical Squamous Cell Carcinoma ◽

Control Group ◽

Vegf Mrna ◽

Node Metastasis ◽

Figo Staging ◽

Vegf Mrna Expression

CSCC is a systemic disease involving polygenic alteration and multiple steps, and HIF and VEGF are closely associated with tumorigenesis. Specimens surgically resected from 64 cases of CSCC and 22 cases of normal cervical tissue were selected randomly to detect the expression of HIF-2αand VEGF in CSCC for exploring their clinical significance; information regarding the age, lymph node metastasis, and FIGO staging were collected as well; expression of HIF-2αand VEGF was detected by qPCR and immunohistochemistry. We found that the expression of HIF-2αand VEGF mRNA in CSCC was significantly higher than that of normal cervical tissues and showed a positive correlation between them. The positive rates of HIF-2αand VEGF protein expression in CSCC and normal cervical tissues were 93.8% and 18.2%, respectively, with correlation between them. The expression of both HIF-2αand VEGF mRNA did not relate closely to age but the FIGO staging and lymph node metastasis. Compared with the counterpart control group, CSCC tissues with high FIGO staging and lymph node metastasis had a higher level of HIF-2αand VEGF mRNA expression. So, HIF-2αand VEGF were overexpressed in CSCC, which has a great clinical significance for its diagnosis.

Download Full-text

Correlation of microelements like plasma copper and zinc concentrations with female infertility

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20172311 ◽

2017 ◽

Vol 6 (6) ◽

pp. 2351

Author(s):

Ritu Bawa ◽

Smita Tyagi

Keyword(s):

Systemic Disease ◽

Venous Blood ◽

Unexplained Infertility ◽

Female Infertility ◽

Reproductive Age ◽

Control Group ◽

Copper And Zinc ◽

Plasma Copper ◽

Zinc Concentrations ◽

The Difference

Background: The aim of present study was to determine the role of trace elements copper and zinc and impairment of infertility.Methods: The study was a randomized, comparative, clinical trial where study group included 74 patients with primary or secondary infertility and control group included 20 patients who were fertile females of reproductive age group having no gynaecological or systemic disease. Venous blood samples were taken and plasma copper and zinc concentrations were measured.Results: In the normal fertile non-pregnant healthy female’s plasma copper ranged from 98.78 - 169.2 mcg% (mean 124.72 mcg%). In patients of unexplained infertility plasma copper was found to be low. It ranged from 63.0 - 145.14 mcg% (mean 95.5 mcg%) difference being statistically significant, (P<0.001). The difference in plasma zinc concentration in both group was not statically significant (P>0.05).Conclusions: Our results show that copper deficiency might have a role to play in the etiogenesis of otherwise unexplained infertility. We can also conclude that zinc deficiency may not play a significant role in female infertility.

Download Full-text