scholarly journals Effect of supplementation of inulin in dietary on lactation performance, rumen fermentation, ruminal microbial profile and metabolites in dairy cows

Author(s):  
Yue Wang ◽  
Xuemei Nan ◽  
Yiguang Zhao ◽  
Linshu Jiang ◽  
Hui Wang ◽  
...  

Abstract Background Inulin is a kind of fructo-oligosaccharide (FOS) derived mainly from Jerusalem artichoke and chicory tubers, which is also a soluble dietary fiber. Inulin has become a scientifically proven prebiotic product with satisfactory effects in improving the structure of intestinal flora, regulating blood lipid and glycemia, etc., in humans and monogastric. However, unlike monogastric animals, ruminal microbes are the largest microbiota in ruminants. The microflora profile and metabolism activity in the rumen are closely related to the health of dairy cows. This study investigated the effects of inulin on rumen fermentation parameters, ruminal microbiome and metabolites, as well as lactation performance and serum indexes in dairy cows. A total of 16 Holstein dairy cows with similar body condition were randomly divided into two groups (n = 8 per group), with inulin addition at 0 and 200 g/d per cow, respectively. Experiment was lasted for 6 weeks including 1 week of adaptation period and 5 weeks of treatment period. At the end of the experimental period, the milk, serum and rumen fluid were sampled and analyzed. The rumen microbiota and metabolites were analyzed via 16S rRNA sequencing and untargeted metabolomics, respectively. Results The supplementation of inulin (200 g/d per cow) increased the milk yield (P = 0.001), milk protein (P = 0.032), lactose rate (P = 0.004) and the proportion of saturated fatty acids (SFA) in milk (P < 0.001), while decreased the proportion of unsaturated fatty acids (USFA) (P = 0.041). Rumen pH (P = 0.040) and concentration of NH3-N (P = 0.024) were decreased, however, acetate (P < 0.001), propionate (P = 0.003), butyrate (P < 0.001) and lactic acid (LA) (P = 0.043) were increased. The total cholesterol (TC) (P = 0.008) and triglycerides (TG) (P = 0.01) in serum were also reduced. Additionally, the inulin addition elevated the relative abundance of several beneficial symbiotic and short-chain fatty acids (SCFA)-producing bacteria, such as Muribaculaceae (FDR-adjusted P < 0.01), Acetitomaculum (FDR-adjusted P = 0.043), and Butyrivibrio (FDR-adjusted P = 0.036) etc., meanwhile elevated the levels of L-Lysine (FDR-adjusted P = 4.24 × 10− 3), L-Proline (FDR-adjusted P = 0.0158), L-Phenylalanine (FDR-adjusted P = 0.027), etc. By contrast, several pathogens and ruminal bacteria abundant in high-fat diets, such as Escherichia-Shigella (FDR-adjusted P = 0.022), Erysipelotrichaceae__UCG-004 (FDR-adjusted P < 0.01) and RF39 (FDR-adjusted P = 0.042) etc., were decreased along with the reduction of LysoPC (18:1(9Z)) (FDR-adjusted P = 1.03 × 10− 3), LysoPC (16:0) (FDR-adjusted P = 0.0108), LysoPC (18:2(9Z, 12Z)) (FDR-adjusted P = 1.65 × 10− 3) and 8-Methylnonenoate etc. Conclusion The results indicated the supplementation of inulin in dietary can increase the relative abundance of commensal microbiota and SCFAs-producing bacteria, meanwhile, upregulate amino acids (AAs) metabolism and downregulate lipid metabolism in rumen of dairy cows, which might further improve lactation performance and the level of serum lipids.

2021 ◽  
Vol 44 (2) ◽  
pp. 152-159
Author(s):  
N. C. Tiven ◽  
L. Hartati ◽  
T. M. Simanjorang

This study was conducted to determine the effect of liquid smoke as a fat protector on unsaturated fatty acids (UFAs) and its effect on rumen fermentation characteristics and microbial activity. Crude palm oil (CPO) was mixed with Prosteo skim milk (1:2), then divided into three treatments i.e., crude palm oil without protection by liquid smoke as a control (P0), crude palm oil protected by 2.5% of liquid smoke (P1), and crude falm oil protected by 5.0% of liquid smoke (P2). For in vitro testing, 300 mg of the feed substrate (elephant grass and bran with the ratio of 60:40) was added with 5% of each crude palm oil preparation of P0, P1, and P2 and put in a fermentor syringe. Then, 30 mL of the mixture of rumen fluid and buffer-minerals solution (1:2) was added into each syringe fermentor and flushed with CO2. The fermentor syringes were incubated in a water bath at 39ᵒC for 48 hours. Variables measured were fatty acid composition, fermentation characteristics, and rumen microbial activity. The data were analyzed by the analysis of variance with a completely randomized design. The results showed that the protection of CPO with liquid smoke in P1 and P2 groups decreased saturated fatty acids (SFAs), but increased (p<0.01) monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), and CMCase activity. Protection of CPO with 2.5% of liquid smoke (P1) significantly increased (p<0.01) fermentation characteristics (NH3 content and pH). It can be concluded that the use of 2.5% of liquid smoke has a better effect on feed fats protection, because it can reduce hydrogenation, increase UFAs, and has no negative effects on fermentation characteristics and microbial activity.


2011 ◽  
Vol 79 (1) ◽  
pp. 16-25 ◽  
Author(s):  
Qendrim Zebeli ◽  
Sarah J Terrill ◽  
Alberto Mazzolari ◽  
Suzanna M Dunn ◽  
Wen Z Yang ◽  
...  

This study evaluated the effects of intraruminal administration ofMegasphaera elsdeniion ruminal fermentation patterns, the profile of plasma metabolites, and milk yield and composition of mid-lactation dairy cows. Eight primiparous, ruminally cannulated Holstein cows were arranged in a paired 2×2 crossover design. Cows were randomly assigned to one of two treatments: 1) intraruminal inoculation of 35 ml suspension per day ofM. elsdeniiATCC 25940 (MEGA), containing 108cfu/ml of bacteria, dissolved in 35 ml of saline (0·15m), or 2) carrier alone (35 ml saline; CTR). Both postprandial and preprandial rumen volatile fatty acids (VFA) and plasma metabolite measurements were analysed. Postprandial VFA patterns were affected the most, with butyrate (P<0·01) and valerate (P<0·01) proportions increasing, and acetate (P<0·01), isobutyrate (P=0·05) and isovalerate (P<0·01) decreasing in MEGA cows. Preprandial data measured at various days showed that MEGA dosage tended to increase the molar proportion of propionate (P=0·09) and lower the acetate to propionate ratio (P=0·07) in the rumen fluid. There was no effect of treatment on rumen pH and on the concentration of lactate in the rumen as well as on selected preprandial plasma metabolites. Postprandial plasma concentrations of cholesterol tended to increase (P=0·07) in MEGA cows compared with CTR. Concentrations of non-esterified fatty acids (NEFA) in the plasma were lower in MEGA cows after the morning feeding (P<0·01). Sampling hour also affected plasma NEFA in this study. Plasma β-hydroxybutyrate (BHBA) were not affected by the treatment (P>0·05); however, after the morning feeding BHBA concentration was increased in both groups of cows. Dry matter intake and milk yield and composition were not affected by treatment. In conclusion, results indicate thatM. elsdeniihas the potential to modulate the rumen fermentation profile in mid-lactation Holstein cows, but these effects were only slightly reflected in changes in plasma metabolites and milk composition.


Animals ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 436
Author(s):  
Ahmed Dawod ◽  
Hamada Ahmed ◽  
Reham Abou-Elkhair ◽  
Hamed T. Elbaz ◽  
Ayman E. Taha ◽  
...  

This study quantifies the effects of extruded linseed and soybean (ELS) dietary supplementation on milk yield, composition, and fatty acid profiles, as well as first-service conception rate in Holstein dairy cows. Seventy-eight open Holstein dairy cows were divided into two groups: (1) a control, which received a basal diet; and (2) a test group, which received a basal diet supplemented with the ELS (650 g/kg of extruded linseed and 150 g/kg of extruded soybean) at a rate of 100 g/kg. In the ELS group, milk yield per day and solid not fat (SNF) yield increased by 3.26% and 0.88%, respectively, in relation to the control. Percentage milk fat decreased significantly by 1.4% in the ELS group when compared with the control. The ELS supplement resulted in a decrease in saturated fatty acids (SFAs) and an increase in monounsaturated (MUFAs) and polyunsaturated fatty acids (PUFAs) in milk. In conclusion, the supplementation of dairy cow feed with 100 g/kg of ELS increases milk yield and milk unsaturated fatty acids (especially MUFAs and PUFAs). ELS supplementation also causes a decrease in percentage fat and SFA levels but does not affect the first-service conception rate or the incidence rate of mastitis.


2011 ◽  
Vol 26 (3) ◽  
pp. 224-229 ◽  
Author(s):  
E. Morales-Almaráz ◽  
B. de la Roza-Delgado ◽  
A. González ◽  
A. Soldado ◽  
M.L. Rodríguez ◽  
...  

AbstractIn recent years, consumer attitudes toward fat of animal origin have changed owing to findings that some milk fatty acids (FAs) are positive for human health, especially conjugated linolenic acid and n-3 FAs. Accordingly, the manipulation of the fat content and FA composition of cows’ milk via nutritional strategies has been an important target for the dairy industry in many countries. Twenty commercial Holstein–Friesian dairy herds of Asturias (northern Spain) with 1106 dairy cows were examined in order to evaluate milk FA profiles under different management systems. These herds were divided into three groups according to management: (1) indoor herds: cows feeding indoors, (2) mixed herds: indoor management system but with at least 6 h of grazing outdoors and (3) outdoor herds: cows allowed 6–18 h of grazing per day. Milk from the indoor herds exhibited the highest concentration of fat (3.57%; P⩽0.01), protein (3.14%; P⩽0.001), lactose (4.76%; P⩽0.01) and urea (29.4 mg dl−1; P⩽0.01). The milk of outdoor herds had a lower (P⩽0.05) content of short-chain FAs than that of the indoor and mixed herds (10.89 versus 11.52 and 11.35 g 100 g−1 FA). The milk of the indoor herds had higher concentrations of saturated fatty acids (SFA) (67.56 g 100 g−1 FA; P⩽0.001) and palmitic and palmitoleic acids (30.16 and 1.82 g 100 g−1 FA, respectively), while that of the mixed and outdoors herds had higher concentrations of unsaturated fatty acids (UFA) (34.58 g 100 g−1 FA; P⩽0.001) and long-chain FAs, especially stearic (13.89 g 100 g−1 FA; P⩽0.01), vaccenic (2.77 g 100 g−1 FA; P⩽0.001), conjugated linoleic (0.92 g 100 g−1 FA; P⩽0.001) and linolenic (0.42 g 100 g−1 FA; P⩽0.001) acids. Results from this study suggest that the incorporation of forage and pasture in the diet of dairy cows can improve the FA profile of milk.


2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Kacper Libera ◽  
Jan Włodarek ◽  
Ewelina Warzych ◽  
Adam Cieślak ◽  
Małgorzata Szumacher-Strabel ◽  
...  

AbstractThe aim of this review is to summarize the current state of knowledge of reproductive performance of high-yielding dairy cows fed a diet supplemented with n-3 fatty acids (FAs), and to recommend a feeding schedule that can increase the success rate in reproduction. Dietary supplementation with fat, especially FA, has become an accepted strategy for supporting the fertility of high-yielding dairy cows. The two main categories of FAs, unsaturated fatty acids (UFAs) and saturated fatty acids (SFAs), exert distinct effects on reproductive functions, with UFA having a conclusively beneficial impact. Polyunsaturated fatty acids (PUFAs) are of particular importance on account of their biological properties. Standard feedstuffs (such as soybean) are rich in n-6 FAs, whereas few feedstuffs contain n-3 FAs. Neither the n-3 nor n-6 PUFAs are synthetized by the animal. Several field experiments have indicated that the recommended schedule of n-3 supplementation should last 160–170 days, from the eighth week before calving to 100 or 120 day postpartum. Supplementation of the diet thus covers the period from the late preantral stage of the follicle, the entire development of the antrum, oocyte ovulation, and fertilization, as well as the development of the preattachment embryo and its implantation. The following effects are among the important beneficial results of supplementation with n-3 FAs: a higher number of antral follicles suitable for ovum pick-up (OPU), larger dominant follicles and corresponding CL, better quality oocytes and embryos, and higher implantation rates resulting from improved uterine environment (e.g., reduced synthesis of luteolytic PGF2α). We conclude that dietary supplementation for high-yielding dairy cows with n-3 FAs supports ovarian functions and helps the embryo to survive in the adverse environment of the reproductive tract of the postpartum cow.


Dairy ◽  
2021 ◽  
Vol 2 (4) ◽  
pp. 716-728
Author(s):  
Senén De La Torre-Santos ◽  
Luis J. Royo ◽  
Adela Martínez-Fernández ◽  
Mario Menéndez-Miranda ◽  
Rocío Rosa-García ◽  
...  

Dairy systems based on grass and forages are widely spread throughout the European Atlantic Arc and they have an influence on milk quality. Likewise, legumes are a key element in the farms to improve cows’ diet and farm feed self-sufficiency. The aim of this study was to evaluate the effect of the legumes in the diet and the feeding system (pasture-based vs. confined) on milk production and composition. An assay was performed with 18 Friesian cows randomized into two management groups (grazing or confined). Three total mixed rations based on Italian ryegrass, faba bean or field pea silages were offered ad libitum for nine continuously housed cows or during two hours after each milking for another nine grazing cows. Regardless of type of silage, grazing cows had higher dry matter intake and milk production than confined cows. Likewise, grazing cows produced milk with a lower concentration of protein and urea than confined cows. The dairy cows fed total mixed rations based on both legume silages had a milk fat with a higher proportion of unsaturated fatty acids, especially with the inclusion of faba bean silage in the diet. The results demonstrate that the profile of fatty acids and antioxidants is related to the feeding system in dairy cows. Grazing directly influenced the composition of milk, decreasing the proportion of saturated fatty acids and increasing the content of unsaturated fatty acids, as CLA, and the antioxidants, as lutein and β-cryptoxanthin.


2002 ◽  
Vol 2002 ◽  
pp. 181-181
Author(s):  
A.L. Lock ◽  
P.C. Garnsworthy

The Δ9 -desaturase system (steroyl-CoA desaturase) involves the addition of a cis double bond between carbons 9 and 10 of fatty acids. The conversion of stearic acid (C18:0) to oleic acid (cis-9 C18:1) is the predominant precursor:product of this enzyme system; conversion of saturated fatty acids (SFA) to mono-unsaturated fatty acids (MUFA) is important in determining the fluidity of milk. In previous studies (Lock & Garnsworthy 2001), we have shown that more than 50% of the oleic acid occurring in milk is produced in the mammary gland via Δ9 -desaturase. We also found that the conversion of trans-11 C18:1 to cis-9, trans-11 conjugated linoleic acid (CLA) accounted for ~80% of milk fat CLA. Increasing the activity of Δ9 -desaturase offers the opportunity of increasing the MUFA content of milk, especially oleic acid, while decreasing its SFA content, as well as increasing the CLA content of milk. Lock & Garnsworthy, (2001) also reported that there were significant differences between individual cows with regard to milk fat CLA content. In an earlier study (Lock & Garnsworthy, 2000) we found that the CLA content of milk varied throughout the year, with highest values occurring when cows received fresh pasture. In view of the significant contribution of Δ9 -desaturase to the CLA and MUFA content of milk, the objective of the work reported here was to investigate individual animal and dietary variation in Δ9 -desaturase activity in the mammary gland of lactating dairy cows.


2018 ◽  
Vol 58 (3) ◽  
pp. 499 ◽  
Author(s):  
H. Benhissi ◽  
I. Beltrán de Heredia ◽  
A. García-Rodríguez

The present study aimed to evaluate the effect of substituting high-linoleic cold-pressed rapeseed or sunflower cakes for palm fat on fatty acids biohydrogenation in an artificial rumen. Three isoproteic and isolipidic diets (forage : concentrate ratio 10 : 90) were evaluated. The three diets consisted of barley straw plus a concentrate mixture supplemented with (1) prilled palm fat (CTR, Control), (2) cold-pressed rapeseed cake (CPRC treatment) or (3) cold-pressed sunflower cake (CPSC treatment) as a lipid source. The assay was conducted using a Rusitec unit consisting of six vessels (two vessels per treatment). After 7-day adaptation period, nutrients disappearance, rumen fermentation parameters and fatty acid profile of rumen digesta were determined for 3 days. CPRC treatment had no effect on nutrients disappearances and rumen fermentation. In contrast, CPSC reduced neutral detergent fibre (P = 0.04), acid detergent fibre (P = 0.01), protein (P = 0.01), organic matter (P < 0.01) and dry matter (P = 0.01) disappearances, compared with CTR and CPRC. CPSC also decreased total volatile fatty acids (P = 0.01) production and shifted rumen fermentation pattern towards lower acetate (P = 0.03) and higher propionate proportion (P = 0.01), in comparison to CTR and CPRC. Both CPRC and CPSC altered the fatty acids composition of ruminal digesta by decreasing the total saturated fatty acids (P < 0.01) and increasing the accumulation of C18:0 (P < 0.01), total C18:1 cis (P < 0.01) and total C18:1 trans (P < 0.01). Vaccenic acid was increased (P < 0.01) 2.18-fold by CPRC and 4.09-fold by CPSC. C18:1 trans-10 :  trans-11 ratio remained constant among treatments (P = 0.31). Rumenic acid was not affected by CPRC but was increased (P = 0.04) 4.25- and 2.83-fold by CPSC compared with CTR and CPRC, respectively. Overall, feeding CPRC or CPSC to ruminants might improve the ruminal fatty acid profile mainly by reducing saturated fatty acids and promoting cis-monounsaturated fatty acids and vaccenic acid accumulation without altering the trans-10 : trans-11 ratio. These changes in rumen fatty acid composition could occur without detrimental effects on ruminal fermentation for CPRC but they might be associated with impaired rumen function for CPSC.


2020 ◽  
Vol 20 (2) ◽  
pp. 38-40
Author(s):  
A. Levitsky ◽  
A. Lapinska ◽  
I. Selivanskaya

The article analyzes the role of essential polyunsaturated fatty acids (PUFA), especially omega-3 series in humans and animals. The biosynthesis of essential PUFA in humans and animals is very limited, so they must be consumed with food (feed). Тhe ratio of omega-3 and omega-6 PUFA is very important. Biomembranes of animal cells contain about 30% PUFA with a ratio of ω-6/ ω-3 1-2. As this ratio increases, the physicochemical properties of biomembranes and the functional activity of their receptors change. The regulatory function of essential PUFA is that in the body under the action of oxygenase enzymes (cyclooxygenase, lipoxygenase) are formed extremely active hormone-like substances (eicosanoids and docosanoids), which affect a number of physiological processes: inflammation, immunity, metabolism. Moreover, ω-6 PUFA form eicosanoids, which have pro-inflammatory, immunosuppressive properties, and ω-3 PUFAs form eicosanoids and docosanoids, which have anti-inflammatory and immunostimulatory properties. Deficiency of essential PUFA, and especially ω-3 PUFA, leads to impaired development of the body and its state of health, which are manifestations of avitaminosis F. Prevention and treatment of avitaminosis F is carried out with drugs that contain PUFA. To create new, more effective vitamin F preparations, it is necessary to reproduce the model of vitamin F deficiency. An experimental model of vitamin F deficiency in white rats kept on a fat –free diet with the addition of coconut oil, which is almost completely free of unsaturated fatty acids, and saturated fatty acids make up almost 99 % of all fatty acids was developed. The total content of ω-6 PUFA (sum of linoleic and arachidonic acids), the content of ω-3 PUFA (α-linolenic, eicosapentaenoic and docosahexaenoic acids) in neutral lipids (triglycerides and cholesterol esters) defined. Тhe content of ω-6 PUFA under the influence of coconut oil decreased by 3.3 times, and the content of ω-3 PUFA - by 7.5 times. Тhe influence of coconut oil, the content of ω-6 PUFA decreased by 2.1 times, and the content of ω-3 PUFA - by 2.8 times. The most strongly reduces the content of ω-3 PUFA, namely eicosapentaenoic, coconut oil, starting from 5 %. Consumption of FFD with a content of 15 % coconut oil reduces the content of eicosapentaenoic acid to zero, ie we have an absolute deficiency of one of the most important essential PUFAs, which determined the presence of vitamin F deficiency.


2014 ◽  
Vol 4 (1) ◽  
pp. 31-39
Author(s):  
Siwitri Kadarsih

The objective was to get beef that contain unsaturated fatty acids (especially omega 3 and 6), so as to improve intelligence, physical health for those who consume. The study design using CRD with 3 treatments, each treatment used 4 Bali cattle aged approximately 1.5 years. Observations were made 8 weeks. Pasta mixed with ginger provided konsentrat. P1 (control); P2 (6% saponification lemuru fish oil, olive oil 1%; rice bran: 37.30%; corn: 62.70%; KLK: 7%, ginger paste: 100 g); P3 (lemuru fish oil saponification 8%, 2% olive oil; rice bran; 37.30; corn: 62.70%; KLK: 7%, ginger paste: 200 g). Konsentrat given in the morning as much as 1% of the weight of the cattle based on dry matter, while the grass given a minimum of 10% of the weight of livestock observation variables include: fatty acid composition of meat. Data the analyzies qualitative. The results of the study showed that the composition of saturated fatty acids in meat decreased and an increase in unsaturated fatty acids, namely linoleic acid (omega 6) and linolenic acid (omega 3), and deikosapenta deikosaheksa acid.Keywords : 


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