scholarly journals Carriage of extended spectrum beta-lactamase-producing Enterobacteriaceae by healthy school children from two remote villages in western Cameroun

2020 ◽  
Author(s):  
Simon Ngamli Fewou ◽  
Wlliam Leorel Nguekap Nankam ◽  
Thérèse Ingrid Lontsi ◽  
Ingrid Cécile Djuikoue
Keyword(s):  
School Children ◽  
Pathogenic Bacteria ◽  
Antibiotic Consumption ◽  
Primary School Children ◽  
Carriage Rate ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Exposed Population ◽  
Comparison Results ◽  
Health Care Structure

Abstract Background: Higher carriage rate of extended spectrum beta-lactamases (ESBL)-producing Enterobacteriaceae have already been reported among healthy community children, thus can increases the risk of developing pathological infection. Since children are the most exposed population due to lack of hygiene knowledge, determining their carriage prevalence will limit the progression or development of those pathologies. The objective of this study was to determine the prevalence of ESBL-producing Enterobacteriaceae carriage among children in remote villages of western Cameroon where healthcare structures are absent and the use of antibiotic consumption rare. Methods: A total of 110 fresh stool samples were collected from 110 healthy primary school children between ages 2 to 5 years old from two remote villages. Upon screening using selective agar media for ESBL, Enterobacteriaceae were identified using the Api 20E gallery. Antibiotic susceptibility was investigated using the disc diffusion technique and the ESBL production was determined using the double-disc synergy test. Chi-square test was used for comparison. Results: Children had no history of hospitalization and had not been subjected to antibiotic treatment three months prior to this study. Data analysis indicated a 22% carriage rate for ESBL-producing Enterobacteriaceae among school children. Overall, 24 (67%) out of 36 isolates were ESBL producers and 15 (61%) out of 24 being Escherichia coli . Other ESBL-producing bacteria were Klebsiella pneumoniae (3%) and Kluyvera spp (3%). We also isolated small proportion of bacteria showing resistance to high level cepholosporinase, which overall represented 33% of the total bacteria isolate. Conclusions: The higher carriage of ESBL-producing Enterobacteriaceae in children from some isolated villages devoid of health care structure highlights the risk for resistance transmission between pathogenic and non-pathogenic bacteria. This study also indicates that farming conditions can induces resistance. The current result may contribute to design a therapeutic policy to curtail the emergence of ESBL-producing Enterobacteriaceae in remote villages in western Cameroon.

scholarly journals Carriage of extended-spectrum beta-lactamase-producing Enterobacteriaceae by healthy school children from two remote villages in western Cameroon

2021 ◽  
Vol 14 (9) ◽  
pp. 3018-3030
Author(s):  
William Lelorel Nankam Nguekap ◽  
Thérèse Ingrid Lontsi ◽  
Anicette Chafa Betbeui ◽  
Simon Ngamli Fewou
Keyword(s):  
Risk Factors ◽  
School Children ◽  
Primary School Children ◽  
Healthy Children ◽  
Extended Spectrum Beta Lactamase ◽  
Factors Associated ◽  
Development Of Resistance ◽  
Extended Spectrum ◽  
Fresh Stool ◽  
High Level

Carriage of extended-spectrum beta-lactamases (ESBL)-producing Enterobacteriaceae by healthy children can increase the risk of developing a lethal pathological infection. The objective of this study was to determine the rate of ESBL-producing Enterobacteriaceae carriage among children in remote villages in western Cameroon. We collected fresh stool samples from 110 healthy primary school children between 2 to 5 years old in two remote villages. The bacteria isolates were characterized using the Api 20E gallery, disc diffusion, and double-disc synergy test. Logistic regression analysis was used to determine the risk factors associated with the carriage of ESBL-producing Enterobacteriaceae. Data analysis indicated that a total of 24 children in 110 (22%) investigated were positive to ESBL-producing Enterobacteriaceae. Moreover, 24 (67%) out of 36 bacteria isolates were ESBL producers and 15 (61%) out of 24 being Escherichia coli. Other ESBL-producing bacteria were Klebsiella pneumoniae (3%) and Kluyvera spp (3%). We also isolated a small proportion of bacteria showing resistance to high-level cephalosporins, which overall represented 33% of the total bacteria isolates. Furthermore, risk factors associated with the carriage of ESBL-producing Enterobacteriaceae were the use of pesticides in agriculture and farming practice. The current result suggests that frequent contact to antibiotics is not the only reason for the development of resistance and confirm that resistance can be induced by chemicals from pesticide origin.

scholarly journals Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon

2016 ◽  
Vol 10 (10) ◽  
pp. 1135-1139 ◽  
Author(s):  
Ingrid Cécile Djuikoue ◽  
Paul-Louis Woerther ◽  
Michel Toukam ◽  
Charles Burdet ◽  
Etienne Ruppé ◽  
...  
Keyword(s):  
Urinary Tract ◽  
Urinary Tract Infections ◽  
Relative Abundance ◽  
Carriage Rate ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Fecal Carriage ◽  
Extended Spectrum ◽  
Associated Risk Factors ◽  
Tract Infections

Introduction: During the last decade, the prevalence of the intestinal carriage of extended spectrum beta-lactamases – producing Escherichia coli (ESBL-E. coli) has continued to increase worldwide in the community, especially in developing countries. Hence, we undertook a study to determine the ESBL-E. coli fecal carriage rate and the associated risk factors in Cameroonian women. Methodology: A total of 86 women suspected of community-acquired urinary tract infections (UTI) were included in 10 health structures from May 2011 to April 2012. After filling a questionnaire, they provided a stool sample that was plated on selective media for ESBL producing bacteria. The identification of strains was obtained with mass spectrometry and the antibiotic susceptibility by disk diffusion in agar media. The ESBL type was determined by PCR. The relative abundance of ESBL-E. coli was measured for positive samples. Eventually, the presence of antibiotics in stool was assessed. Results: The carriage rate of ESBL-E. coli was 57/86 (66.3%). Phenotypic and molecular characterization showed that all ESBL-E. coli strains contained group 1 CTX-M enzymes. Multivariate analysis showed that ESBL-E. coli fecal carriage was associated with the presence of antibiotics in stools (p < 0.05). Although not significant, mean ESBL relative abundance tended to be higher in patients with antibiotic exposure. Conclusions: Our results show that the carriage of ESBL-E. coli fecal carriage in women with UTI suspicion from the Cameroonian community is extremely high and associated with recent antibiotic intake.

scholarly journals Extended-spectrum beta-lactamase (ESBL)-positive Escherichia coli presence in urban aquatic environments in Kanpur, India

2020 ◽  
Vol 18 (5) ◽  
pp. 849-854
Author(s):  
Ann Johnson ◽  
Olivia Ginn ◽  
Aaron Bivins ◽  
Lucas Rocha-Melogno ◽  
Sachchida Nand Tripathi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Aquatic Environment ◽  
Significant Risk ◽  
Uttar Pradesh ◽  
Environmental Water ◽  
Antibiotic Consumption ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum

Abstract In India, high rates of antibiotic consumption and poor sanitation infrastructure combine to pose a significant risk to the public through the environmental transmission of antimicrobial resistance (AMR). The WHO has declared extended-spectrum beta-lactamase (ESBL)-positive Escherichia coli a key indicator for the surveillance of AMR worldwide. In the current study, we measured the prevalence of AMR bacteria in an urban aquatic environment in India by detecting metabolically active ESBL-positive E. coli. Water samples were collected in duplicate from 16 representative environmental water sources including open canals, drains, and rivers around Kanpur, Uttar Pradesh. We detected culturable E. coli in environmental water at 11 (69%) of the sites. Out of the 11 sites that were positive for culturable E. coli, ESBL-producing E. coli was observed at 7 (64%). The prevalence of ESBL-producing E. coli detected in the urban aquatic environment suggests a threat of AMR bacteria to this region.

scholarly journals Carriage Rate of Neisseria meningitidis, Antibiotic Susceptibility Pattern and Associated Risk Factors among Primary School Children in Gondar town, Northwest Ethiopia

2019 ◽  
Author(s):  
Zelalem Tefera ◽  
Feleke Mekonnen ◽  
Moges Tiruneh ◽  
Teshome Belachew
Keyword(s):  
Risk Factors ◽  
Neisseria Meningitidis ◽  
School Children ◽  
Northwest Ethiopia ◽  
Primary School Children ◽  
P Value ◽  
Susceptibility Pattern ◽  
Carriage Rate ◽  
Gondar Town ◽  
Associated Risk Factors

Abstract Background: Globally, in 2012, 1.2 million estimated cases were reported with ~135,000 deaths annually. In Ethiopia, specifically in our study area, limited information is found on the oropharyngeal carriage, antimicrobial resistance pattern, and associated risk factors for N. meningitidis among school children. So, the aim of this study was to assess oropharyngeal carriage rate of N. meningitidis, antibiotic susceptibility pattern and determinants among primary school children in Gondar town, Northwest Ethiopia. Methods: A cross sectional study was conducted from January to April, 2019 in Gondar town. Multi stage simple random sampling technique was used. A total of 524 oropharyngeal swabs were collected using sterile plastic cotton swabs. Modified Thayer Martin media was used for primary isolation. Antimicrobial susceptibility pattern was done based on Kirby-Bauer method on Muller-Hinton agar supplemented with 5% sheep blood. Multidrug resistance was defined as resistance of an isolate to two or more antimicrobial classes tested. Logistic regression model was used to see the association between dependent variables (Carriage rate of Neisseria meningitidis, Serogroups of Neisseria meningitidis and Antimicrobial susceptibility patterns) and independent variables (Socio-demographic data and risk factors). Variables with a P- value ≤ 0.2 during bivariable analysis was taken to multivariable analysis to check significant association of meningococcal carriage with risk factors. Finally, a P-value < 0.05 was considered as statistically significant. Data was summarized using numbers, percentages and tables. Results: A total of 53(10.1%) (CI: 7.6-12.8) N. meningitidis isolates were identified. Serogroup A 13 (24.5%) was the most prevalent followed by Y/W135 11(20.7%) whereas serogroup B 4(7.6%) was the least identified serotype. Meningococcal isolates were resistant to ciprofloxacin (45.3%) and trimethoprim-sulfamethoxazole (73.6%). Overall, most of meningococcal isolates showed about 32(60.4%) multidrug resistance. Meningococcal carriage rate was significantly associated with family size, tonsillectomy, passive smoking, number of students per class, sharing utensils, history of visiting healthcare institutions, and indoor kitchen. Conclusion: This study highlights the need for reinforcement of case-based, laboratory confirmed surveillance of N. meningitidis carriage in Ethiopian elementary school students to enable mapping of distribution of serotypes of the causative organisms across the country and determine the current potential necessity of vaccination.

scholarly journals Speech therapy aspect of improving the quality of education of schoolchildren in a capital megapolis

2021 ◽  
Vol 98 ◽  
pp. 01016
Author(s):  
Yuliia Vyatleva ◽  
Natalia Grigorenko ◽  
Yuliia Pokrovskaya ◽  
Natalia Bal
Keyword(s):  
Primary School ◽  
School Children ◽  
The State ◽  
Russian Language ◽  
Primary School Children ◽  
Theoretical Research ◽  
Comparison Results ◽  
The Impact ◽  
The Russian Language

The Russian language, as the state language, is given an honorable and important role, uniting all nationalities together. For its mission to serve the unity, solidarity, and mutual understanding of all the peoples of Russia, a mandatory national educational program has been approved, operating throughout the territory of the Russian Federation. The present article is dealing with the problem of mastering the Russian language. The goal of the research is to study the etiology and specifics of writing disorders in primary school children studying at general education school and to develop differentiated strategies for teaching pupils with various manifestations of dysgraphia. Methods used during the preparation of the article included theoretical research, such as learning, generalization, analysis, synthesis, axiomatics, as well as empirical techniques, namely, observation and comparison. Results and novelty of the research consist of clarifying information about the state of the problem of writing disorders in contemporary schoolchildren, updating scientific ideas about the contingent of primary school children in need of correctional assistance from specialists; applying an interdisciplinary approach to the study of the etiology, mechanisms, causes, and specifics of various manifestations of dysgraphia in primary general school children; supplementing the scientific data on the impact of didactogenia on the quality of learning writing and the formation of dysgraphia in pupils with difficulties in the assimilation of the program learning material on the Russian language; as well developing high-performance speech technologies for the early detection and elimination of violations of written language and difficulties in learning academic courses of the Russian language.

scholarly journals Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae in hospital and community settings in Chad

2019 ◽  
Vol 8 (1) ◽  
Author(s):  
Oumar Ouchar Mahamat ◽  
Abdelsalam Tidjani ◽  
Manon Lounnas ◽  
Mallorie Hide ◽  
Julio Benavides ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pathogenic Bacteria ◽  
Hospitalized Patients ◽  
Diffusion Method ◽  
Community Settings ◽  
Carriage Rate ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Fecal Carriage ◽  
Extended Spectrum

Abstract Background Fecal carriage of extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-PE) remains poorly documented in Africa. The objective of this study was to determine the prevalence of ESBL-PE fecal carriage in Chad. Methods In total, 200 fresh stool samples were collected from 100 healthy community volunteers and 100 hospitalized patients from January to March 2017. After screening using ESBL-selective agar plates and species identification by MALDI-TOF mass spectrometry, antibiotic susceptibility was tested using the disk diffusion method, and ESBL production confirmed with the double-disc synergy test. The different ESBL genes in potential ESBL-producing isolates were detected by PCR and double stranded DNA sequencing. Escherichia coli phylogenetic groups were determined using a PCR-based method. Results ESBL-PE fecal carriage prevalence was 44.5% (51% among hospitalized patients vs 38% among healthy volunteers; p < 0.05). ESBL-producing isolates were mostly Escherichia coli (64/89) and Klebsiella pneumoniae (16/89). PCR and sequencing showed that 98.8% (87/89) of ESBL-PE harbored blaCTX-M genes: blaCTX-M-15 in 94.25% (82/87) and blaCTX-M-14 in 5.75% (5/87). Phylogroup determination by quadruplex PCR indicated that ESBL-producing E. coli isolates belonged to group A (n = 17; 27%), C (n = 17; 27%), B2 (n = 9; 14%), B1 (n = 8; 13%), D (n = 8; 13%), E (n = 1; 1.6%), and F (n = 1; 1.6%). The ST131 clone was identified in 100% (9/9) of E. coli B2 strains. Conclusions The high fecal carriage rate of ESBL-PE associated with CTX-M-15 in hospital and community settings of Chad highlights the risk for resistance transmission between non-pathogenic and pathogenic bacteria.

scholarly journals Prevalence of Extended-Spectrum Beta-Lactamases-Producing Microorganisms in Patients Admitted at KRRH, Southwestern Uganda

2017 ◽  
Vol 2017 ◽  
pp. 1-5 ◽  
Author(s):  
Baguma Andrew ◽  
Atek Kagirita ◽  
Joel Bazira
Keyword(s):  
Healthcare Workers ◽  
Pathogenic Bacteria ◽  
Regional Referral Hospital ◽  
Beta Lactamases ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Diffusion Technique ◽  
Extended Spectrum Beta Lactamases ◽  
Southwestern Uganda

The emergence of extended-spectrum beta-lactamase- (ESBL-) producing pathogenic bacteria at Kabale Regional Referral Hospital (KRRH), located in southwestern Uganda, is of great concern: a phenomenon that worries clinicians and other healthcare workers due to the serious threat they pose to patients. This current study aimed at determining the phenotypic detection of ESBL-producing strains ofE. coli, Klebsiellasp., andProteussp. isolated from clinical specimens and their prevalence in patients admitted at KRRH. We used combined disc diffusion technique to detect and establish the presence of ESBLs-producing bacteria. Of the 100 tested bacterial isolates, 89 (89%) were identified as ESBL-producing bacteria.Klebsiellasp. predominated in the samples (46 (52%)), presenting the highest frequency of ESBLs producing followed byE. coli(39 (44%)) andProteus mirabilis(4 (4.5%)) from the combined disk diffusion.

scholarly journals High Carrying Rate of Extended-Spectrum Beta-Lactamase (ESBL) Producing Enterobacteriaceae by Slaughterhouse Workers in Lomé, Togo in 2019

2020 ◽  
pp. 30-41
Author(s):  
Amivi Mawussi Godonou ◽  
Fifonsi Adjidossi Gbeasor-Komlanvi ◽  
Novissi Tsogbale ◽  
Fiali Lack ◽  
Sika Dossim ◽  
...  
Keyword(s):  
Clavulanic Acid ◽  
Health Concern ◽  
Diffusion Method ◽  
Food Chains ◽  
Beta Lactamase ◽  
Bacterial Strains ◽  
Carriage Rate ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum

Introduction: Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL) represent a real public health concern because of their spread. The role of agri-food chains in transmitting of digestive ESBL-producing bacterial strains in the community, was demonstrated but little work was done in our settings (Togo, west Africa). The aim of this study was to estimate the rate of digestive carrying ESBL producing enterobacteriacae in slaughterhouse workers in Lomé, Togo. Material and Methods: This was a cross-sectional study carried out in three slaughterhouses in Lomé. Fresh stools of 60 slaughterhouse workers and socio-demographic data were collected during the period of September to October 2019 after obtaining the consent of each participant. The bacterial strains of interest were isolated on the selective medium Purple Bromocresol + Ceftazidime at 6µg/l. UriselectÒ and API 20E media were used for identification. Antibiotic susceptibility test was performed in Mueller-Hinton agar plate diffusion method (Kirby Bauer technic) and according to CASFM-EUCAST recommendations. Results: The digestive carriage rate of ESBL producing enterobacteriacae among professionals of three slaughterhouses of Lomé was 80% (n=48/60). Escherichia coli was the main bacteria 78.2% (n = 43/55) followed by Klebsiella pneumoniae 16.4% (n = 9/55) and Enterobacter cloacae 5.4% (n = 3/55). The antibiotic profile of ESBL producing enterobacteriacae showed resistance to Amoxycillin + Clavulanic Acid (26%), Ticarcillin + Clavulanic Acid (86%), Piperacillin + Tazobactam (14%), Cefoxitin (7%) Ciprofloxacin (63%), Levofloxacin (49%), Nalidixic Acid (42%), Chloramphenicol (33%), Gentamicin (21%), Sulfamethoxazole-Trimetoprim (93%). These bacteria were 100% sensitive to Imipenem, Ertapenem, Amikacin and Fosfomycin. Conclusion: This study revealed a very high carriage rate of ESBL producing Enterobacteriacae among Slaughterhouse Workers in Lomé. It confirmed the major potential role of the agri-food chains in the spread of ESBL producing bacteria in the Community.

scholarly journals Extended-Spectrum Beta-Lactamase Producing-Escherichia coli Isolated From Irrigation Waters and Produce in Ecuador

2021 ◽  
Vol 12 ◽  
Author(s):  
Lorena Montero ◽  
Jorge Irazabal ◽  
Paul Cardenas ◽  
Jay P. Graham ◽  
Gabriel Trueba
Keyword(s):  
Escherichia Coli ◽  
Irrigation Water ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Resistant Bacteria ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Fruit Samples ◽  
Contaminated Streams

In cities across the globe, the majority of wastewater – that includes drug resistant and pathogenic bacteria among other contaminants – is released into streams untreated. This water is often subsequently used for irrigation of pastures and produce. This use of wastewater-contaminated streams allows antibiotic-resistant bacteria to potentially cycle back to humans through agricultural products. In this study, we investigated the prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolated from produce and irrigation water across 17 provinces of Ecuador. A total of 117 vegetable samples, 119 fruit samples, and 38 irrigation water samples were analyzed. Results showed that 11% of the samples were positive for E. coli including 11 irrigation water samples (29%), and samples of 13 vegetables (11%), and 11 fruits (9%). Among the 165 E. coli isolates cultured, 96 (58%) had the ESBL phenotype, and 58% of ESBL producing E. coli came from irrigation water samples, 11% from vegetables, and 30% from fruits. The blaCTX–M–55, blaCTX–M 65, and blaCTX–M 15 genes were the most frequently found gene associated with the ESBL phenotype and coincided with the blaCTX–M alleles associated with human infections in Ecuador. Three isolates had the mcr-1 gene which is responsible for colistin resistance. This report provides evidence of the potential role of irrigation water in the growing antimicrobial resistance crisis in Ecuador.

Sign in / Sign up

Export Citation Format

Share Document