scholarly journals Effect of Lead on the Development and Nutrient Accumulation Ability of Musca Domestica

2021 ◽  
Author(s):  
Wanqiang Wang ◽  
Wenzheng Tang ◽  
Jie Zhang ◽  
Yiting Wen ◽  
Xiaoping Wang ◽  
...  
Keyword(s):  
Musca Domestica ◽  
Lead Exposure ◽  
Digestive Enzyme ◽  
Total Lipid Content ◽  
The Body ◽  
Lead Contamination ◽  
Total Protein Content ◽  
Lead Pollution ◽  
Nutrient Accumulation ◽  
Adult Growth

Abstract Lead pollution poses a threat to the survival of animals, plants and humans. Environmental pollution conversion with insects is a new type of environmental management. Housefly (Musca domestica) may be an efficient, economical, and safe choice for lead pollution control. However, this needs to make sure that houseflies are strongly resistant to lead contamination. In this study, housefly larvae, pupae and adult growth parameters and adult fecundity under lead treatment were counted, and changes in digestive enzyme activities and gene expression levels were detected to comprehensively analyze the tolerance of housefly to lead exposure. As a result, lead accumulated in the larvae under 5000 mg/kg lead treatment and caused a decrease in the survival rate of the larvae, extension of the developmental duration, decrease of the body weight, and dynamic changes in oviposition dynamics. The total sugar and total protein content of the larvae decreased, while the amylase and trypsin activities decreased, the total lipid content increased, and the lipase activity increased, the expression of all digestive enzyme genes changed. 20 mg/kg lead treatment has no significant effect on the growth and development of housefly. In conclusion, lead exposure mainly affected the growth rate of larvae and adult fecundity by affecting the efficiency of housefly nutrient accumulation.

scholarly journals Fatty acid composition and parasitism of European sardine (Sardina pilchardus) and European anchovy (Engraulis encrasicolus) populations in the northern Catalan Sea in the context of changing environmental conditions

2020 ◽  
Author(s):  
Sebastian Biton-Porsmoguer ◽  
Ricard Bou ◽  
Elsa Lloret ◽  
Manuel Alcaide ◽  
Josep Lloret
Keyword(s):  
Fatty Acid ◽  
Environmental Conditions ◽  
Total Lipid Content ◽  
Taxonomic Composition ◽  
The Body ◽  
Adjacent Area ◽  
Poor Condition ◽  
Lipid Dynamics ◽  
Phytoplankton Communities ◽  
The Status

AbstractThe status of sardine and anchovy populations in the northern Mediterranean Sea has been declining in recent decades. In this study, fatty acids and parasitism at different reproductive and feeding stages in these two species were assessed using specimens caught along the northern Catalan coast, in order to assess the links between lipid dynamics, reproduction and feeding in these two species, and to contribute towards an explanation of the potential causes of the current situation of the stocks. The results support the use of fatty acid levels as indicators of the body condition of sardine and anchovy at different reproductive and feeding stages, as well as that of the pelagic environmental conditions. In particular, the relatively low n-3 PUFA levels (which are crucial for reproductive success) found in spawning sardines compared to spawning anchovies indicate a poorer reproductive health status of sardine. By comparing the current total lipid content values with those recorded in other Mediterranean and North Atlantic areas, and, others from more than ten years ago, in the adjacent area of the Gulf of Lion, our study reveals the persistent poor condition of sardine and anchovy in the northern Catalan Sea. Furthermore, the low levels of diatom fatty acid markers observed throughout the spawning and non-spawning seasons in both sardine and anchovy, indicate a diet poor in diatoms. Moreover, the results indicate that it is very unlikely that parasitism is a significant factor in the decline in condition of sardine and anchovy in the northern Catalan Sea. In fact, the results suggest that the current poor condition of sardine and anchovy in the northern Catalan Sea has been exacerbated by a decrease in plankton productivity and/or a shift in the taxonomic composition of phytoplankton communities, adding to the ongoing effects of overfishing.

scholarly journals Monitoring the effects of exposure to lead and cadmium in working and living environment through standard biochemical blood parameters and liver endonucleases activity

2011 ◽  
Vol 65 (4) ◽  
pp. 403-409 ◽  
Author(s):  
Ruzica Nikolic ◽  
Jasmina Jovanovic ◽  
Gordana Kocic ◽  
Tatjana Cvetkovic ◽  
Svetlana Stojanovic ◽  
...  
Keyword(s):  
Liver Enzymes ◽  
Harmful Effect ◽  
Toxic Metal ◽  
Combustion Products ◽  
Blood Parameters ◽  
Living Environment ◽  
The Body ◽  
Lead Contamination ◽  
Lead And Cadmium ◽  
High Level

Heavy metals as pollutants in the working and living environment are a serious health and environmental problem because they are toxic, non-biodegradable, accumulate in living systems and have a long half-life in soil. Sources of lead contamination are combustion products in the chemical industry and metallurgy, industrial waste water, landfills, traffic etc. Lead enters into the body via the food chain and drinking water. In the body lead is deposited in the liver, kidneys, brain and mineral tissues. Excretion of lead causes damage to the epithelial cells of certain organs. High level exposure to cadmium is usually the result of environmental pollution by human activities. Exposure to cadmium can lead to acute and chronic tissue damage of various organs, including liver and kidneys in humans and in animals. In this paper we analyzed the effects of lead and cadmium exposure, in working and living environment, on the model system of experimental animals, particularly the activity of certain liver enzymes, acid and alkaline DNase, and standard biochemical blood parameters. The study showed that lead and cadmium significantly affect the protein content, red blood cells, hemoglobin and hematocrit, and the activity of liver enzymes. This harmful effect of this toxic metal can be reduced by the supplements.

scholarly journals Dynamics of individual indicators of protein metabolism in the body of broiler chickens on the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”

2020 ◽  
Vol 3 (2) ◽  
pp. 42-46
Author(s):  
V. G. Stoyanovskyy ◽  
M. O. Shevchuk ◽  
I. A. Kolomiiets ◽  
V. A. Kolotnytskyy
Keyword(s):  
Total Protein ◽  
Protein Metabolism ◽  
Broiler Chickens ◽  
Stress Factor ◽  
The Body ◽  
Control Group ◽  
Total Protein Content ◽  
Combined Stress ◽  
Stages Of Development ◽  
Γ Globulins

The body of  broiler chickens is characterized by a high metabolism, which promotes rapid growth and development, but their performance indicators are largely related to housing conditions, which are known to include a number of technological stressors. With the development of stress in the body of poultry changes the course of metabolic processes, which causes changes in all types of metabolism, including protein. The aim of the study was to determine the changes in individual indicators of protein metabolism in the body of broiler chickens against the background of combined stress when included in the diet “Reasil Humic Vet” + “Laktin” and “Reasil Humic Health”. At 13th day of life, all clinically healthy poultry were exposed to combined stress – revaccination (intranasal Newcastle disease) plus cold stress (for 60 minutes by air conditioning and 5 °C in the vivarium). Material for research was selected for 3 days after the action of the stress factor (stage of anxiety), 13, 20 and 26 days after the action of the stress factor (the resistance stage). The material for the study was blood plasma, which determined the concentration of total protein, as well as the content of albumin, globulin fraction (α1, α2, β, γ). It was found that 3 days after the action of stress in chickens of Control group the development of adaptive reactions is manifested by the stability of the total protein content and redistribution of the fractional composition of plasma globulins in the form of increased albumin and α2-globulins. At different stages of development of the stage of resistance in birds of Control group, the content of total protein decreases by an average of 14.6 % due to the content of albumin by 6.9 % and α2-globulins – by 15.9 %, against the background of increasing α1- and γ-globulins by 23.1 and 33.5 % with the stabilization of individual studied indicators at the final stages of development of the resistance stage. The use in the diet of broilers feed “Reasil Humic Vet”, probiotic feed supplement “Laktin”, feed “Reasil Humic Health” under the influence of complex stress helps to increase the intensity of protein metabolism in poultry with the development of adaptation syndrome, as evidenced by the growth of total protein on average by 37.8 % (P < 0.05) and albumin – by 17.0 % (P < 0.05), which indicates an increase in the intensity of protein-synthesizing properties of the organism. In different periods of stress in the blood of broiler chickens Research groups there is an increase in the ratio of individual protein fractions, especially the content of γ-globulins - an average of 21.3 % (P < 0.05), which indicates an increase in the immune status of their body with a predominance of numerical values in the Research 1 group of poultry.

Lead induced oxidative DNA damage in battery-recycling child workers from Bangladesh

2018 ◽  
Vol 34 (4) ◽  
pp. 213-218 ◽  
Author(s):  
Mohammad Arif ◽  
MM Towhidul Islam ◽  
Hossain Uddin Shekhar
Keyword(s):  
Dna Damage ◽  
Lead Exposure ◽  
Oxidative Dna Damage ◽  
Lead Concentration ◽  
Threshold Level ◽  
Protein Carbonyl ◽  
The Body ◽  
Lipid Peroxidation Product ◽  
Protein Carbonyl Content ◽  
Battery Recycling

Lead exposure can damage cells directly by effecting DNA or indirectly by modifying proteins and enzymes. In Bangladesh, many working children are exposed to a very high level of lead during their early life due to their involvement with lead-oriented professions. This imposes a severe threat to the growth and development of the children. Therefore to study the effect of lead, we enrolled 60 age-matched male children, from an area of old Dhaka city, where battery-recycling shops are located, depending on their blood lead concentration. If the children had a plasma lead concentration above the WHO recommended threshold level of 10 µg/dl, we grouped them as test subjects and others as control subjects to determine the effect of lead on different biochemical parameters of the body. Compared to the controls, acculumlation of the lipid peroxidation product, malondialdehyde, increased significantly in test subjects ( p < 0.01). Lead exposure also increased the protein carbonyl content ( p < 0.05) and significantly decreased the plasma glutathione levels of test subjects compared to the controls ( p < 0.05). While comparing the lead-exposed group against controls, it was found that the percentage of damaged DNA, as measured using the Comet assay, significantly increased in tail ( p < 0.01) and decreased in head regions. All of these results suggest that high-plasma lead content may induce an oxidative stress to the study population, which may lead to DNA damage.

scholarly journals The body composition and enzymes of carbohydrate metabolism of Varroa destructor

2013 ◽  
Vol 57 (2) ◽  
pp. 93-100 ◽  
Author(s):  
Elżbieta Łopieńska–Biernat ◽  
Małgorzata Dmitryjuk ◽  
Ewa Zaobidna ◽  
Zbigniew Lipiński ◽  
Krystyna Żółtowska
Keyword(s):  
Varroa Destructor ◽  
Glycogen Synthase ◽  
Glycogen Metabolism ◽  
Cholesterol Content ◽  
The Body ◽  
Total Protein Content ◽  
Basic Composition ◽  
Metabolism Enzymes ◽  
Parasitic Mite ◽  
A Minor

Abstract The aim of the present research was to determine the basic composition of the organic compounds present in extracts from Varroa destructor, a parasitic mite of the honeybee. The total protein content was 9.16 ± 0.82 mg/100 mg of body weight, lipid content was 9.81 ± 1.99 mg/100 mg, and carbohydrate content was 26.67 ± 4.52 mg/100 mg. The triacylglycerol content was 2.40 ± 0.86 mg/100 mg and the cholesterol content was 0.14 ± 0.02 mg/100 mg. Thin layer chromatography indicated that phospholipids comprised the major part of the lipid component; cephalins (78%), cerebrosides (16%), and lecithins (6%) were identified in the phospholipid pool. Glucose (23.6 ± 4.52 mg/100 mg) was the main carbohydrate, followed by glycogen (5.43 ± 1.23 mg/100 mg) and trehalose (0.35 ± 0.07 mg/100 mg). Enzyme-linked immunosorbent assays detected two major glycogen metabolism enzymes, glycogen phosphorylase and glycogen synthase. Among the enzymes metabolising disaccharides, maltase (24.7 ± 2.38 μmol/mg protein) and trehalase (14.81 ± 5.21 μmol/mg protein) presented the highest activity. Saccharose and lactose were hydrolysed to a minor extent. These are the first measurements of the basic composition of the mite body. Although these data are not exhaustive, they may serve as the basis for further research on the metabolism of V. destructor, particularly concerning lipid metabolism.

In Vivo X-Ray Fluorescence Analysis for Medical Diagnosis

1979 ◽  
Vol 23 ◽  
pp. 185-191 ◽  
Author(s):  
L. Ahlgren ◽  
T. Grönberg ◽  
S. Mattsson
Keyword(s):  
Body Burden ◽  
Fluorescence Analysis ◽  
Industrial Applications ◽  
The Body ◽  
Lead Contamination ◽  
X Ray ◽  
Sensitive Function ◽  
Total Body ◽  
Medical Interest

Occupational exposure to lead is common in many industrial applications and hence it is of considerable medical interest to control the body-burden of lead in living man. More than 90 % of the lead in the body is concentrated in bone and hence in vivo measurements of the lead in the skeleton should give the most satisfactory way for estimating the body-burden. The routine method used today for checking on lead contamination is that of measurements on blood samples. However, since the concentration of lead in the blood is a sensitive function of the actual exposure conditions, this method provides only a poor indication of the total body-burden and the integrated lead exposure.

scholarly journals Physiological Changes of Parachlorella Kessleri TY02 in Lipid Accumulation under Nitrogen Stress

2019 ◽  
Vol 16 (7) ◽  
pp. 1188 ◽  
Author(s):  
Yifan Gao ◽  
Jia Feng ◽  
Junping Lv ◽  
Qi Liu ◽  
Fangru Nan ◽  
...  
Keyword(s):  
Total Lipid ◽  
Total Protein ◽  
Lipid Accumulation ◽  
Lipid Synthesis ◽  
Total Lipid Content ◽  
Nitrogen Stress ◽  
Total Protein Content ◽  
Total Carbohydrate ◽  
Chlorophyll Fluorescence Parameters ◽  
Parachlorella Kessleri

In order to study the effects of nitrogen stress on the lipid synthesis of Parachlorella kessleri TY02 and to understand the changes in growth, photosynthetic pigments, total protein and total carbohydrate contents during lipid accumulation, the cells of the strain were cultured in nitrogen-deficient (N−) and nitrogen-rich (N+) media for one week. Changes in cell growth, chlorophyll content, chlorophyll fluorescence parameters, neutral lipid and total lipid content, total protein content and total carbohydrate content were measured and analyzed. The results showed that, under nitrogen stress, the algal strain grew slowly, and chlorophyll and total protein contents decreased, while total carbohydrate and total lipid contents increased. This indicated that, under nitrogen stress, most of the carbon flowed to the synthesis of lipids and carbohydrates. Meanwhile, reducing the nitrogen content was a relatively economical and easy to operate method of promoting lipid accumulation.

EFFECTS OF DIET ON THE COMPOSITION OF BODY FAT IN MUSCA DOMESTICA L.

1966 ◽  
Vol 44 (5) ◽  
pp. 775-779 ◽  
Author(s):  
J. S. Barlow
Keyword(s):  
Fatty Acids ◽  
Oleic Acid ◽  
Linoleic Acid ◽  
Musca Domestica ◽  
Growth And Development ◽  
Palmitoleic Acid ◽  
Larval Growth ◽  
Dietary Fatty Acids ◽  
The Body ◽  
High Concentrations

Larvae of Musca domestica L. were reared on chemically defined diets to which various fatty acids were added. The concentration of lipids in the body was directly related to the amount of fatty acid in the diet. Lack of oleic acid was tantamount in the following respects to lack of all fatty acids: high concentrations of palmitic, stearic, and particularly palmitoleic acid; low concentration of oleic acid. Linoleic acid could not be detected in the body fats unless it was fed. Fats had little or no effect on rate of larval growth and development. These results are compared with the results of a previous study of the effects of dietary fatty acids on the composition of the body fats of the parasitic dipteron, Pseudosarcophaga affinis Auct. nee Fallén.

Psychrobacter sp. 17-1 enhances growth and survival in early postlarvae of white shrimp, Penaeus vannamei Boone, 1931 (Decapoda, Penaeidae)

Crustaceana ◽  
2016 ◽  
Vol 89 (13) ◽  
pp. 1467-1484 ◽  
Author(s):  
R. Franco ◽  
A. Arenal ◽  
L. Martín ◽  
Y. Martínez ◽  
D. Santiesteban ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Digestive Enzyme ◽  
Enzymatic Activities ◽  
White Shrimp ◽  
Control Group ◽  
Total Protein Content ◽  
Penaeus Vannamei ◽  
Untreated Control Group ◽  
Growth And Survival ◽  
Digestive Enzyme Activities

The effects of Psychrobacter sp., isolated from the gut of shrimp Penaeus vannamei Boone, 1931, on growth, digestive enzyme activities, total protein content, osmotic stress resistance, immunity and disease resistance of postlarvae were investigated. For enzymatic activities assays whole postlarvae were homogenized. Shrimp subjected to Psychrobacter sp. treatment showed higher weight (40%) and length (5.6%) than that observed in untreated animals. Compared to the untreated control group, levels of protease and amylase activities in postlarvae, were higher in the probiotic group. Postlarvae treated with Psychrobacter sp. showed significantly increase in the survival compared to the untreated ones after osmotic stress. Nitric oxide metabolite levels and the enzymatic activities of phenoloxidase, superoxide dismutase and peroxidase from treated postlarvae were significantly higher than untreated postlarvae. Increased survival was also found after challenge with Aeromonas sp. This is the first study that demonstrates the probiotic effect of Psychrobacter sp. in P. vannamei postlarvae.

scholarly journals Molecular dynamics of FMRP and other RNA-binding proteins in MEG-01 differentiation: the role of mRNP complexes in non-neuronal development

2016 ◽  
Vol 94 (6) ◽  
pp. 597-608 ◽  
Author(s):  
M. McCoy ◽  
D. Poliquin-Duchesneau ◽  
F. Corbin
Keyword(s):  
Protein Content ◽  
Binding Proteins ◽  
Rna Binding ◽  
De Novo ◽  
Rna Binding Proteins ◽  
Morphological Changes ◽  
Fragile X ◽  
Cellular Protein ◽  
The Body ◽  
Total Protein Content

Asymmetrically differentiating cells are formed with the aid of RNA-binding proteins (RBPs), which can bind, stabilize, regulate, and transport target mRNAs. The loss of RBPs in neurons may lead to severe neurodevelopmental diseases such as the Fragile X Syndrome with the absence of the Fragile X Mental Retardation Protein (FMRP). Because the latter is ubiquitous and shares many similarities with other RBPs involved in the development of peripheral cells, we suggest that FMRP would have a role in the differentiation of all tissues where it is expressed. A MEG-01 differentiation model was, therefore, established to study the global developmental functions of FMRP. PMA induction of MEG-01 cells causes important morphological changes driven by cytoskeletal dynamics. Cytoskeleton change and colocalization analyses were performed by confocal microscopy and sucrose gradient fractionation. Total cellular protein content and de novo synthesis were also analyzed. Microtubular transport mediates the displacement of FMRP and other RBP-containing mRNP complexes towards regions of the cell in development. De novo protein synthesis decreases significantly upon differentiation and total protein content composition is altered. Because those results are comparable with those obtained in neurons, the absence of FMRP would have significant consequences in cells everywhere in the body. The latter should be further investigated to give a better understanding of the systemic implications of imbalances of FMRP and other functionally similar RBPs.

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