scholarly journals Circulating miRNAs and Cytokines Uncover Novel Physiopathological Mechanisms of COVID-19

2021 ◽  
Author(s):  
Pedro Martínez-Fleta ◽  
Paula Vera-Tomé ◽  
María Jiménez-Fernández ◽  
Silvia Requena ◽  
Emilia Roy-Vallejo ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Multivariate Analysis ◽  
Elderly Patients ◽  
Plasma Protein ◽  
Plasma Levels ◽  
Circulating Mirnas ◽  
Life Threatening ◽  
Plasma Mirnas ◽  
Independent Cohort

Abstract Coronavirus Disease 2019 (COVID-19) pneumonia is a life-threatening infectious disease, especially for elderly patients with multiple comorbidities. Despite enormous efforts to understand its underlying etiopathogenic mechanisms, most of them remain elusive. In this study, we compared differential plasma miRNAs and cytokines profiles between COVID-19 and other community-acquired pneumonias (CAP). A first screening and subsequent validation assays in an independent cohort of patients revealed a signature of 15 dysregulated miRNAs between COVID-19 and CAP patients. Additionally, multivariate analysis displayed a combination of 4 miRNAs (miR-106b-5p, miR-221-3p, miR-25-3p and miR-30a-5p) that significantly discriminated between both pathologies. Search for targets of these miRNAs, combined with plasma protein measurements, identified a differential cytokine signature between COVID-19 and CAP that included EGFR, CXCL12 and IL-10. Significant differences were also detected in plasma levels of CXCL12, IL-17, TIMP-2 and IL-21R between mild and severe COVID-19 patients. These findings provide new insights into the etiopathological mechanisms underlying COVID-19.

scholarly journals A Differential Signature of Circulating miRNAs and Cytokines Between COVID-19 and Community-Acquired Pneumonia Uncovers Novel Physiopathological Mechanisms of COVID-19

2022 ◽  
Vol 12 ◽  
Author(s):  
Pedro Martínez-Fleta ◽  
Paula Vera-Tomé ◽  
María Jiménez-Fernández ◽  
Silvia Requena ◽  
Emilia Roy-Vallejo ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Multivariate Analysis ◽  
Elderly Patients ◽  
Plasma Protein ◽  
Plasma Levels ◽  
Community Acquired Pneumonia ◽  
Circulating Mirnas ◽  
Life Threatening ◽  
Plasma Mirnas ◽  
Independent Cohort

Coronavirus Disease 2019 (COVID-19) pneumonia is a life-threatening infectious disease, especially for elderly patients with multiple comorbidities. Despite enormous efforts to understand its underlying etiopathogenic mechanisms, most of them remain elusive. In this study, we compared differential plasma miRNAs and cytokines profiles between COVID-19 and other community-acquired pneumonias (CAP). A first screening and subsequent validation assays in an independent cohort of patients revealed a signature of 15 dysregulated miRNAs between COVID-19 and CAP patients. Additionally, multivariate analysis displayed a combination of 4 miRNAs (miR-106b-5p, miR-221-3p, miR-25-3p and miR-30a-5p) that significantly discriminated between both pathologies. Search for targets of these miRNAs, combined with plasma protein measurements, identified a differential cytokine signature between COVID-19 and CAP that included EGFR, CXCL12 and IL-10. Significant differences were also detected in plasma levels of CXCL12, IL-17, TIMP-2 and IL-21R between mild and severe COVID-19 patients. These findings provide new insights into the etiopathological mechanisms underlying COVID-19.

Monitoring of tricyclic antidepressant plasma levels and clinical response: a review of the literature. Part II

1989 ◽  
Vol 4 (2) ◽  
pp. 81-90 ◽  
Author(s):  
I.R. De Oliveira ◽  
P.A.S. Do Prado-Lima ◽  
B. Samuel-Lajeunesse
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Elderly Patients ◽  
Plasma Levels ◽  
Tricyclic Antidepressant ◽  
Review Of The Literature ◽  
Central Nervous System Toxicity ◽  
Routine Measurement ◽  
System Toxicity

SummaryPart II of this paper contains some general considerations on tricyclic antidepressant (TCA) monitoring. Long-term assessment of TCA plasma levels is advised by the few existent studies, although each of these focusses on different aspects. Cardiovascular and central nervous system toxicity is reviewed as well as pharmacokinetics and the importance of protein binding. Some consideration is also given to their use in elderly patients. The authors conclude that although available data support its usefulness in many situations, routine measurement of TCA levels is not warranted.

scholarly journals Clinical relevance of glomerular IgM deposition in patients with lupus nephritis

2021 ◽  
Author(s):  
Fengmei Wang ◽  
Jirong Yu ◽  
Lei zhang ◽  
Yan Zhang ◽  
Jie Zhang ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Lupus Nephritis ◽  
Serum Creatinine ◽  
Plasma Levels ◽  
Clinical Relevance ◽  
Alternative Pathway ◽  
Clinicopathological Parameters ◽  
Low Density ◽  
Lower Plasma ◽  
Low Levels

Abstract Background The aim of the study was to investigate the clinical relevance of IgM deposition in patients with LN in a large cohort. Results 217 patients with renal biopsy–proven lupus nephritis were enrolled. The associations between glomerular IgM deposition and clinicopathological parameters were further analyzed. IgM deposition was positively correlated with glomerular C1q and C3 deposition moderately (r = 0.436, P < 0.001; r = 0.408, P < 0.001, respectively), and inversely correlated with plasma levels of C3 and CFH mildly (r=-0.138, P = 0.043; r=-0.147, P = 0.037, respectively). By multivariate analysis, we found that glomerular IgM deposition independently contributes to glomerular C3 deposition in patients with lupus nephritis (OR = 2.002, 95% CI: 1.295–3.094, P = 0.002). In addition, we also found that patients with IgM 0+-2 + had similar plasma CFH levels, but in patients with IgM3+-4+, plasma CFH levels were significantly lower (300.4 ± 155.8µg/ml vs. 429.9 ± 187.5µg/ml, P < 0.001). Furthermore, patients with high density of glomerular IgM and low levels of CFH had heavier proteinuria, higher serum creatinine and lower plasma C3 levels (5.7 ± 3.1g/d vs. 4.7 ± 3.5g/d, P = 0.037;150.1 ± 121.0µmol/L vs. 105.6 ± 97.1µmol/L, P = 0.005; 0.3 ± 0.2µg/L vs. 0.4 ± 0.2µg/L, P = 0.04, respectively), comparing with those with low density of glomerular IgM and low levels of CFH. Conclusions Our results suggested IgM might bind to injury-associated epitopes and be involved in disease progression and provided a possible relevance of CFH and IgM in the process of alternative pathway (AP) activation.

scholarly journals Health, Food Consumption, Social Economy, and Stunting Incidency in Timor Leste

2017 ◽  
Vol 13 (2) ◽  
Author(s):  
Cipriano Do Rosario Pacheco ◽  
Intje Picauly ◽  
Mindo Sinaga
Keyword(s):  
Infectious Disease ◽  
Multivariate Analysis ◽  
Social Economy ◽  
Health Sector ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Bivariate Analysis ◽  
Health Food ◽  
Cross Sectional ◽  
Timor Leste

Stunting can affect the health of toddlers. The prevalence of stunting in Timor Leste in 2014 is 50.2%, whereas in Aileu District is 52.3%. Stunting can interfere intellectual growth and development. The purpose of this study was to analyze the relationship between stunting determinants. The research design was cross sectional. The population of this study was toddlers 24-59 months old, with a sample size of 102 people. Sampling technique used was simple random sampling. Data were analyzed bivariately using simple linear regression, while multivariate analysis was performed using multiple logistic regressions. Bivariate analysis revealed variables that have significant relationship with stunting: immunization (p=0.026), infectious disease (p=0.049), income (p=0.003), total family member (p=0.045). Multivariate analysis obtained two variables as determinant factor of stunting: infectious disease (p=0.025; OR=0.355; 95% CI=0.144-0.877), income (p=0.028; OR=0.332; 95% CI=0.124-0.887). The incidence of stunting in toddlers in Aileu District is very serious (54.9%). Toddlers with stunting demonstrated the adverse effects of stunting on growth, development, and the quality of human resources. Stunting is not only a health sector issue. It is necessary to have appropriate interventions with early and liable management through various related sectors.

scholarly journals PENGALAMAN CAREGIVER DALAM MERAWAT PASIEN PALIATIF DI PANTI WERDHA TULUS KASIH

2021 ◽  
Vol 5 (1) ◽  
pp. 19
Author(s):  
Putri Raissa Hamidah ◽  
Nurhayati Siagian
Keyword(s):  
Elderly Patients ◽  
Old Age ◽  
Sampling Technique ◽  
Development Stage ◽  
Daily Activities ◽  
Final Phase ◽  
Interview Methods ◽  
Life Threatening ◽  
Caregiver Experiences ◽  
Palliative Patients

Late adulthood or known as old age, is the final phase of the life development stage. Old age is often identified with a period of decline in various bodily functions and an impact on helplessness. Because of this, many elderly suffer from incurable and life-threatening diseases known as palliative diseases. Many families of elderly patients who have palliative diseases feel unable to care for their patients at home, and finally, choose to leave elderly palliative patients at the Wredha Home because patients depend on the help of others for care to fulfill their needs and to carry out their daily activities accompanied by a caregiver at the Wredha Home. This reasearch was to ascertain an expertises felt by caregivers in caring for palliative patients at the Tulus Kasih Home. This research uses qualitative methods, data collection using interview methods with structured guides, and a purposive sampling technique that includes four participants. The result of this study covered six themes: the caregiver's understanding of palliative patients, what the palliative patients needs, what difficulties the caregiver experiences when giving action to elderly palliative patients, the caregiver's response when having difficulty caring for palliative patients, what efforts are made by the caregiver when having difficulty caring for palliative patients, and the efforts made by the caregiver in increasing the action for palliative patients.

scholarly journals Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance

2021 ◽  
Vol 12 ◽  
Author(s):  
Haixiong Liu ◽  
Wenjin Xu ◽  
Jiying Feng ◽  
Hong Ma ◽  
Jianbin Zhang ◽  
...  
Keyword(s):  
Roc Curves ◽  
Brain Diseases ◽  
Circulating Mirnas ◽  
Heroin Use ◽  
Non Invasive ◽  
Heroin Use Disorder ◽  
Subjective Reports ◽  
The Brain ◽  
Potential Use ◽  
Plasma Mirnas

Heroin use disorder is a chronic and relapsing disease that induces persistent changes in the brain. The diagnoses of heroin use disorders are mainly based on subjective reports and no valid biomarkers available. Recent researches have revealed that circulating miRNAs are useful non-invasive biomarkers for diagnosing brain diseases such as Alzheimer's disease, multiple sclerosis, schizophrenia, and bipolar disorder. However, studies on circulating miRNAs for the diagnosis of heroin use disorders are rarely reported. In this study, we investigated the differential expression of plasma miRNAs in 57 heroin-dependent patients. Based on literature research and microarray analysis, two candidate miRNAs, miR-320a and let-7b-5p, were selected and analyzed by quantitative real-time RT-PCR. The results showed miR-320a and let-7b were significantly upregulated in plasma of the heroin-dependent patients compared to that in healthy controls. The area under curves (AUCs) of receiver operating characteristic (ROC) curves of miR-320a and let-7b-5p were 0.748 and 0.758, respectively. The sensitivities of miR-320a and let-7b-5p were 71.9 and 70.2%, while the specificities of miR-320a and let-7b-5p were 76.1 and 78.3%, respectively. The combination of these two miRNAs predicted heron dependence with an AUC of 0.782 (95% CI 0.687–0.876), with 73.7% sensitivity and 82.6% specificity. Our findings suggest a potential use for circulating miRNAs as biomarkers for the diagnosis of heroin abuse.

scholarly journals Clinical Role of Lung Ultrasound for the Diagnosis and Prognosis of Coronavirus Disease Pneumonia in Elderly Patients: A Pivotal Study

Gerontology ◽  
2020 ◽  
pp. 1-9
Author(s):  
Guerino Recinella ◽  
Giovanni Marasco ◽  
Manuel Tufoni ◽  
Mara Brizi ◽  
Eleonora Evangelisti ◽  
...  
Keyword(s):  
Multivariate Analysis ◽  
Elderly Patients ◽  
Lung Ultrasound ◽  
Univariate Analysis ◽  
Hospital Death ◽  
Clinical Role ◽  
Diagnosis And Prognosis ◽  
Hospitalized Elderly ◽  
Novel Coronavirus

<b><i>Background:</i></b> Lung ultrasound (LUS) showed a promising role in the diagnosis and monitoring of patients hospitalized for novel coronavirus disease (COVID-19). However, no data are available on its role in elderly patients. <b><i>Aims:</i></b> The aim of this study was to evaluate the diagnostic and prognostic role of LUS in elderly patients hospitalized for severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) pneumonia. <b><i>Methods:</i></b> Consecutive elderly patients (age &#x3e;65 years) hospitalized for COVID-19 were enrolled. Demographics, laboratory, comorbidity, and the clinical features of the patients were collected. All patients underwent LUS on admission to the ward. LUS characteristics have been analyzed. Uni- and multivariate analyses to evaluate predictors for in-hospital death were performed. <b><i>Results:</i></b> Thirty-seven hospitalized elderly patients (19 men) with a diagnosis of SARS-CoV-2 infection were consecutively enrolled. The median age was 82 years (interquartile range 74.5–93.5). Ultrasound alterations were found in all patients enrolled; inhomogeneous interstitial syndrome with spared areas (91.9%) and pleural alterations (100%) were the most frequent findings. At univariate analysis, LUS score (hazard ratio [HR] 1.168, 95% CI 1.049–1.301) and pleural effusions (HR 3.995, 95% CI 1.056–15.110) were associated with in-hospital death. At multivariate analysis, only LUS score (HR 1.168, 95% CI 1.049–1.301) was independelty associated with in-hospital death. The LUS score’s best cutoff for distinguishing patients experiencing in-hospital death was 17 (at multivariate analysis LUS score ≥17, HR 4.827, 95% CI 1.452–16.040). In-hospital death was significantly different according to the LUS score cutoff of 17 (<i>p</i> = 0.0046). <b><i>Conclusion:</i></b> LUS could play a role in the diagnosis and prognosis in elderly patients hospitalized for SARS-CoV-2 infection.

Rescue carotid puncture for ischemic stroke treated by endovascular therapy: a multicentric analysis and systematic review

2020 ◽  
pp. neurintsurg-2020-016725
Author(s):  
Julien Allard ◽  
Sam Ghazanfari ◽  
Mehdi Mahmoudi ◽  
Julien Labreuche ◽  
Simon Escalard ◽  
...  
Keyword(s):  
Systematic Review ◽  
Ischemic Stroke ◽  
Elderly Patients ◽  
Functional Outcome ◽  
Endovascular Therapy ◽  
Older Patients ◽  
Intravenous Thrombolysis ◽  
Review Of The Literature ◽  
Femoral Access ◽  
Life Threatening

BackgroundEndovascular therapy (EVT) for acute ischemic stroke (AIS) can be challenging in older patients with supra-aortic tortuosity. Rescue carotid puncture (RCP) can be an alternative in case of supra-aortic catheterization failure by femoral access, but data regarding RCP are scarce. We sought to investigate the feasibility, effectiveness and safety of RCP for AIS treated by EVT.MethodsPatients treated by EVT with RCP were included from January 2012 to December 2019 in the Endovascular Treatment in Ischemic Stroke (ETIS) multicentric registry. Main outcomes included reperfusion rates (≥TICI2B), 3 month functional outcome (modified Rankin Scale) and 3 month mortality. We also performed an additional systematic review of the literature according to the PRISMA checklist to summarize previous studies on RCP.Results25 patients treated by EVT with RCP were included from the ETIS registry. RCP mainly concerned elderly patients (median age 85 years, range 73–92) with supra-aortic tortuosity (n=16 (64%)). Intravenous thrombolysis (IVT) was used for nine patients (36%). Successful reperfusion was achieved in 64%, 87.5% of patients were dependent at 3 months, and 3 month mortality was 45.8%. The systematic review yielded comparable results. In pooled individual data, there was a shift toward better functional outcome in patients with successful reperfusion (median (IQR) 4 (2–6) vs 6 (4–6), p=0.011).ConclusionRCP mainly concerned elderly patients admitted for AIS with anterior LVO with supra-aortic tortuosity. The procedure seemed feasible, notably for patients treated with IVT, and led to significant reperfusion rates at the end of procedure, but with pronounced unfavorable outcomes at 3 months. RCP should be performed under general anesthesia to avoid life-threatening complications and ensure airways safety. Finally, RCP led to low rates of closure complications, emphasizing that this concern should not withhold RCP, if indicated.

scholarly journals The Eye as a Window to Systemic Infectious Diseases: Old Enemies, New Imaging

2019 ◽  
Vol 8 (9) ◽  
pp. 1392 ◽  
Author(s):  
Pirani ◽  
Pelliccioni ◽  
De Turris ◽  
Rosati ◽  
Franceschi ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Infectious Diseases ◽  
Clinical Evaluation ◽  
Multimodal Imaging ◽  
Ocular Involvement ◽  
Tertiary Referral Center ◽  
Imaging Examination ◽  
Previous Diagnosis ◽  
Life Threatening ◽  
Examination Methods

Background: Syphilis, tuberculosis and toxoplasmosis are major infectious diseases worldwide; all of them are multisystem pathologies and share a possible ocular involvement. In this context, a fundamental help for the definitive diagnosis is provided by the ophthalmologist, through clinical evaluation and with the aid of a multimodal imaging examination. Methods: We hereby describe selected cases who came to our attention and were visited in our eye clinic. In all clinics, the use of retinal and optic disc multimodal imaging during ophthalmological evaluation allowed to make a diagnosis of an infectious disease. Results: In our tertiary referral center more than 60 patients with syphilis, tuberculosis and toxoplasmosis have been evaluated in the last two years: In 60% of cases the ophthalmological evaluation was secondary to a previous diagnosis of an infectious disease, while in the remaining cases the ophthalmologist, with the help of a multimodal imaging examination and clinical evaluation, represented the physician who leads to the diagnosis. Conclusion: Our results confirm how in these life-threatening pathologies a prompt diagnosis is mandatory and may benefit from a multidisciplinary and multimodal imaging approach, especially during ophthalmological evaluation.

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