scholarly journals Identification of Raspberry Cultivars by Nonradioactive DNA Fingerprinting

HortScience ◽  
1992 ◽  
Vol 27 (10) ◽  
pp. 1108-1110 ◽  
Author(s):  
Jean-Guy Parent ◽  
Danièl Pagé
Keyword(s):  
Alkaline Phosphatase ◽  
Developmental Stage ◽  
Dna Fingerprinting ◽  
Restriction Enzymes ◽  
Growing Season ◽  
Rubus Idaeus ◽  
Band Pattern ◽  
Red Raspberry ◽  
Genetic Fingerprinting ◽  
Unique Band

Characterization and identification of 13 red raspberry (Rubus idaeus L.) and two purple raspberry (R. × neglectus Peck) cultivars were obtained by nonradioactive genetic fingerprinting. DNA from leaves was digested with Hae III and Hin f I restriction enzymes and probed with alkaline phosphatase-labeled oligonucleotide. All tested cultivars could be identified by a unique band pattern. No differences were noted within cultivars when the reproducibility of the fingerprints was evaluated by analyzing the effects of age of the raspberry plantation, developmental stage during the growing season, or position of the sampled leaf on stem. These results suggest that simple nonradioactive DNA fingerprinting can be routinely used to identify raspberry cultivars.

scholarly journals Alternate-year Production Is Not Profitable in Washington Floricane Red Raspberry Systems

2021 ◽  
pp. 1-4
Author(s):  
Lisa Wasko DeVetter ◽  
Suzette Galinato ◽  
Troy Kortus ◽  
Jonathan Maberry
Keyword(s):  
Growing Season ◽  
Rubus Idaeus ◽  
Selective Removal ◽  
Economic Viability ◽  
Red Raspberry ◽  
And Training

Floricane red raspberry (Rubus idaeus) produces biennial canes that are traditionally managed by annual selective removal of previously fruited floricanes and training of primocanes that will bear fruit in the next growing season. This process of pruning and training is labor intensive and costly, and growers would benefit from more economical methods of pruning and training. This 6-year project evaluated the economic viability of alternate-year (AY) production in a commercial floricane red raspberry field in northwest Washington and compared it to traditional, every-year (EY) production to assess whether the former could save costs. Despite savings from reduced chemicals, fertilizers, labor, general farm supplies, and other variable costs, the overall benefits of AY production were not enough to offset losses in revenue resulting from reduced yields under the conditions of this experiment in northwest Washington.

scholarly journals DIURNAL PHOTOSYNTHESIS AND STARCH DEPOSITION IN LEAVES OF FRUITING AND DEFLOWERED RED RASPBERRY CANES

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 654b-654
Author(s):  
Stephen F. Klauer ◽  
Chuhe Chen ◽  
Paul W. Foote ◽  
J. Scott Cameron
Keyword(s):  
Cross Sections ◽  
Fruit Development ◽  
Dry Weight ◽  
Growing Season ◽  
Rubus Idaeus ◽  
Total Soluble Protein ◽  
Starch Accumulation ◽  
Red Raspberry ◽  
Fruit Maturity ◽  
Diurnal Photosynthesis

On four dates during the 1991 growing season, gas exchange rates were measured on the same middle leaflets every 3 h from 7am-10pm from deflowered (DF) and fruiting (F) red raspberry (Rubus idaeus L. cv. “Meeker”) canes. Concurrently, the adjacent side leaflets were sampled for anatomical starch determination. The dates corresponded to the late anthesis/early green fruit, early red fruit, late red fruit, and post fruit maturity stages of the growing season. For all dates, CO2 assimilation (A) was highest from 7-10am, lowest at 4pm, and increased at 7pm. Overall A peaked during fruit development. Leaves of F canes had greater A than leaves of DF canes during fruit development, but rates were similar after fruit maturity. Starch accumulation in leaf cross-sections generally followed the diurnal pattern observed for A. Starch appeared heaviest from 7am-lpm and often showed an increase from 7-10pm. Leaves from DF canes generally had a greater accumulation of starch. Seasonally, leaf starch from F canes appeared greatest at late anthesis, decreased during fruit development and was very low post fruit maturity. Leaf starch in DF canes appeared greatest at the late anthesis and late red fruit stages. DF leaves had greater dry weight accumulation than F leaves during the red fruit stages. A Western blot showed that Rubisco levels as a percentage of total soluble protein were higher during fruit development and decreased after fruit maturity.

scholarly journals 741 PB 067 14CO2 MOVEMENT BETWEEN FLORICANES AND PRIMOCANES IN RED RASPBERRY DURING THE FRUITING CYCLE

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 539c-539
Author(s):  
Stenhen F. Klauer ◽  
J. Scott Cameron ◽  
Paul W. Foote
Keyword(s):  
Liquid Scintillation ◽  
Developmental Stages ◽  
Growing Season ◽  
Rubus Idaeus ◽  
Red Raspberry ◽  
Green Fruit ◽  
Maturity Stages ◽  
Fruit Maturity ◽  
Physiological Studies

Results from previous cultural and physiological studies of red raspberry suggest that primocanes compete with floricanes for light, nutrients and/or photoassimilates. This study was undertaken to determine whether this competition might be reflected in the actual translocation of photoassimilates between the two types of canes. In 1993, pairs of greenhouse grown, potted red raspberry (Rubus idaeus L.) plants contaming one or two floricanes and numerous primocanes were labeled with 14CO2 on four dates corresponding with early anthesis, green fruit, red fruit and post fruit maturity stages of the growing season. For each experiment, either a floricane or a primocane was exposed to 92.5μCi 14CO2 within a sealed bag. After 24 hours, the bag was removed and the presence of label was monitored for up to 11 days. Activity was determined using liquid scintillation. At all developmental stages 14C moved from the labeled floricane to primocanes that were from 2.5 cm to 1.5 m tall and to the roots. Movement was quickest and relatively greatest at early anthesis, dccreascd during fruiting, and was still occuring at 2 months after fruit maturity. Small amounts of label were detected in roots of labeled primocanes at all stages, but trace amounts were present in fruit and other primocanes only at post fruit maturity.

Impact of an Open Trellis on Canopy Growth, Light Interception, Yield, and Leaf Physiology of Red Raspberry (Rubus idaeus)

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 468b-468
Author(s):  
Stephen F. Klauer ◽  
J. Scott Cameron ◽  
Chuhe Chen
Keyword(s):  
Leaf Area ◽  
Total Nitrogen ◽  
Dry Weight ◽  
Light Interception ◽  
Rubus Idaeus ◽  
Above Ground Biomass ◽  
Red Raspberry ◽  
Leaf Physiology ◽  
Total Dry Weight ◽  
Upper Third

After promising results were obtained with an open-style split trellis (two top wires) in its initial year, two new trials were established in 1997 in northwest (Lynden) and southwest (Woodland) Washington. For the split trellis, actual yields were 33% (machine-picked 1/2 season) and 17% (hand-picked) greater, respectively, for the two locations compared to the conventional trellis (one top wire). In Woodland, canes from the split trellis had 33% more berries, 55% more laterals, 69% more leaves, and 25% greater leaf area compared with the conventional trellis. Greatest enhancement of these components was in the upper third of the canopy. Laterals were also shorter in this area of the split canopy, but there was no difference in average total length of lateral/cane between trellis types. Total dry weight/cane was 22% greater in the split trellis, but component partitioning/cane was consistent between the two systems with fruit + laterals (43%) having the greatest above-ground biomass, followed by the stem (30% to 33%) and the leaves (21% to 22%). Measurement of canopy width, circumference, and light interception showed that the split-trellis canopy filled in more quickly, and was larger from preanthesis through postharvest. Light interception near the top of the split canopy was 30% greater 1 month before harvest with 98% interception near the top and middle of that canopy. There was no difference between the trellis types in leaf CO2 assimilation, spectra, or fluorescence through the fruiting season, or in total nitrogen of postharvest primocane leaves.

scholarly journals Genome-Wide Association Study (GWAS) for Examining the Genomics Controlling Prickle Production in Red Raspberry (Rubus idaeus L.)

Agronomy ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 27
Author(s):  
Archana Khadgi ◽  
Courtney A. Weber
Keyword(s):  
Association Study ◽  
Genome Wide Association Study ◽  
Genomic Region ◽  
Rubus Idaeus ◽  
Genome Wide Association ◽  
Nucleotide Polymorphisms ◽  
Red Raspberry ◽  
Genome Wide ◽  
A Genome ◽  
Genotype By Sequencing

Red raspberry (Rubus idaeus L.) is an expanding high-value berry crop worldwide. The presence of prickles, outgrowths of epidermal tissues lacking vasculature, on the canes, petioles, and undersides of leaves complicates both field management and harvest. The utilization of cultivars with fewer prickles or prickle-free canes simplifies production. A previously generated population segregating for prickles utilizing the s locus between the prickle-free cultivar Joan J (ss) and the prickled cultivar Caroline (Ss) was analyzed to identify the genomic region associated with prickle development in red raspberry. Genotype by sequencing (GBS) was combined with a genome-wide association study (GWAS) using fixed and random model circulating probability unification (FarmCPU) to analyze 8474 single nucleotide polymorphisms (SNPs) and identify significant markers associated with the prickle-free trait. A total of four SNPs were identified on chromosome 4 that were associated with the phenotype and were located near or in annotated genes. This study demonstrates how association genetics can be used to decipher the genetic control of important horticultural traits in Rubus, and provides valuable information about the genomic region and potential genes underlying the prickle-free trait.

scholarly journals Combining QTL Mapping and Gene Expression Analysis to Elucidate the Genetic Control of ‘Crumbly’ Fruit in Red Raspberry (Rubus idaeus L.)

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 794
Author(s):  
Luca M. Scolari ◽  
Robert D. Hancock ◽  
Pete E. Hedley ◽  
Jenny Morris ◽  
Kay Smith ◽  
...  
Keyword(s):  
Genetic Control ◽  
Developmental Disorder ◽  
Molecular Mechanisms ◽  
Developmental Stages ◽  
Microarray Experiment ◽  
Rubus Idaeus ◽  
Red Raspberry ◽  
Genotype By Sequencing ◽  
First Time ◽  
Selection Of

‘Crumbly’ fruit is a developmental disorder in raspberry that results in malformed and unsaleable fruits. For the first time, we define two distinct crumbly phenotypes as part of this work. A consistent crumbly fruit phenotype affecting the majority of fruits every season, which we refer to as crumbly fruit disorder (CFD) and a second phenotype where symptoms vary across seasons as malformed fruit disorder (MFD). Here, segregation of crumbly fruit of the MFD phenotype was examined in a full-sib family and three QTL (Quantitative Trait Loci) were identified on a high density GbS (Genotype by Sequencing) linkage map. This included a new QTL and more accurate location of two previously identified QTLs. A microarray experiment using normal and crumbly fruit at three different developmental stages identified several genes that were differentially expressed between the crumbly and non-crumbly phenotypes within the three QTL. Analysis of gene function highlighted the importance of processes that compromise ovule fertilization as triggers of crumbly fruit. These candidate genes provided insights regarding the molecular mechanisms involved in the genetic control of crumbly fruit in red raspberry. This study will contribute to new breeding strategies and diagnostics through the selection of molecular markers associated with the crumbly trait.

scholarly journals The Effect of Irradiance, Defoliation, and Bulb Size on Flowering of Nerine bowdenii W. Watson (Amaryllidaceae)

1996 ◽  
Vol 121 (1) ◽  
pp. 115-122 ◽  
Author(s):  
K.I. Theron ◽  
G. Jacobs
Keyword(s):  
Developmental Stage ◽  
Dry Weight ◽  
Growing Season ◽  
Growth Unit ◽  
Growing Seasons ◽  
Number Of Leaves

Large Nerine bowdenii bulbs (>14 cm in circumference) were exposed to low ligbt intensities for different periods during two successive growing seasons. The flowering percentage and number of florets in the current season's inflorescence were recorded at anthesis. Small and large bulbs were subjected to continual defoliation starting at different times during the growing season. Bulbs were dissected at planting (26 Sept. 1992) and on 12 Jan. 1993 (nondefoliated control bulbs) to determine growth and developmental stage. At anthesis, inflorescences were harvested and the florets per inflorescence were counted. After anthesis in the fall, all bulbs were dissected and the following variables recorded: 1) percentage flowering, quiescence, or abortion of the current season's inflorescence; 2) developmental stage of quiescent inflorescences; 3) number of florets in the outermost inflorescence; 4) developmental stage of the innermost inflorescence; 5) number of leaves or leaf bases in each growth unit; 6) number of daughter bulbs; and 7) dry weight of new leaf bases. There were three reasons for nonflowering of the bulbs, viz., failure to initiate an inflorescence, inflorescences remaining quiescent, and inflorescence abortion. Individual florets that had not reached stage “Late G” (gynoecium elongated, carpels fused) at the start of rapid inflorescence elongation aborted. The more florets that aborted, the greater the probability that the entire inflorescence aborted. The inflorescence was more vulnerable to stress during the first half of the growing season due to its relatively weak position in the hierarchy of sinks within the bulb.

scholarly journals Performance of Three Primocane-fruiting Red Raspberry Cultivars in Ontario and Québec

1993 ◽  
Vol 118 (3) ◽  
pp. 388-392 ◽  
Author(s):  
Jean-Pierre Privé ◽  
J.A. Sullivan ◽  
J.T.A. Proctor ◽  
O.B. Allen
Keyword(s):  
Maximum Yield ◽  
Rubus Idaeus ◽  
Yield Potential ◽  
Red Raspberry ◽  
Genotype X Environment ◽  
Genotype X Environment Interactions ◽  
Consistent Performance

The influence of genotype x environment interactions on the performance of `Autumn Bliss' `Heritage' and `Redwing' primocane-fruiting (PF) red raspberry (Rubus idaeus L.) cultivars was studied at six sites across Ontario and Quebec during 1989 and 1990. Cultivar × location × year interactions were found for most vegetative and reproductive components analyzed. `Autumn Bliss' had the most consistent performance of the three cultivars in all location/year combinations, while `Redwing' varied greatly between environments. `Heritage' was always the latest-bearing of the three cultivars and failed to achieve its maximum yield potential in many of the northern locations.

scholarly journals Breaking Dormancy in Red Raspberry with Hot Water Treatment and its Effects on Cold Hardiness

2006 ◽  
Vol 131 (2) ◽  
pp. 209-213 ◽  
Author(s):  
Pauliina Palonen ◽  
Leena Lindén
Keyword(s):  
Water Treatment ◽  
Cold Hardiness ◽  
Treatment Temperature ◽  
Rubus Idaeus ◽  
Hot Water ◽  
Bud Dormancy ◽  
Hot Water Treatment ◽  
Red Raspberry ◽  
Breaking Dormancy ◽  
Water Treatments

`Maurin Makea', `Muskoka', ` Ottawa', and `Preussen' red raspberry (Rubus idaeus L.) canes were collected from the field and subjected to different hot water treatments (20, 35, 40, 45, and 50 °C) to determine if endodormancy could be removed by a near lethal stress. Estimation of days for 50% budbreak (DD50) was found useful for describing the state of bud dormancy in the samples. Bud dormancy was broken in `Ottawa' by immersing the canes in 45 °C water for 2 hours, in `Maurin Makea' by treating the canes in 40 °C water, and in `Preussen' by both 40 and 45 °C treatments. The influence of this treatment on dormancy and cold hardiness at different times of the winter was further examined using `Ottawa' raspberry. The treatment removed bud dormancy most effectively in October, when the samples were in deepest dormancy. A slight effect was observed in November, but no effect in January. During ecodormancy in February the treatment delayed budbreak. Hot water treatment reduced cold hardiness of `Ottawa' canes by 8 to 15 °C, and that of buds by 9 to 13 °C during both endo- and ecodormancy. Based on the capacity of buds and canes to reacclimate, recovery from the stress treatment was possible at temperatures ≥4 °C. Loss of cold hardiness was caused by high treatment temperature itself and was not related to breaking of dormancy in samples. This finding suggests that dormancy and cold hardiness are physiologically unconnected in raspberry.

Sign in / Sign up

Export Citation Format

Share Document