scholarly journals Influence of Phostrade Ca on Color Development and Anthocyanin Content of ‘Braeburn’ Apple (Malus domestica Borkh.)

HortScience ◽  
2013 ◽  
Vol 48 (2) ◽  
pp. 193-199 ◽  
Author(s):  
Jan Bizjak ◽  
Nika Weber ◽  
Maja Mikulic-Petkovsek ◽  
Ana Slatnar ◽  
Franci Stampar ◽  
...  
Keyword(s):  
Hydroxycinnamic Acids ◽  
Anthocyanin Synthesis ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Total Sugars ◽  
Primary And Secondary Metabolites ◽  
Color Development ◽  
Commercial Harvest ◽  
Significant Difference ◽  
High Concentrations

The influence of two foliar applications of Phostrade Ca, which contains high concentrations of phosphorus and minor amounts of calcium and nitrogen, on color development and selected primary and secondary metabolites was investigated during advanced maturation of ‘Braeburn’ apple. Changes of hydroxycinnamic acids, flavanols, dihydrochalcones, flavonols, and anthocyanins were monitored six times during the advanced ripening until technological maturity of the fruits. Additionally, the changes in the chromatic values a*, h°, and the lightness coefficient L* were recorded weekly. The colorimetric parameters showed a significant difference in the intensity of red coloration between the treated and untreated apples. Spraying with Phostrade Ca also resulted in a significant increase in most individual sugars, total sugars, and concentration of anthocyanins and flavonols. Moreover, the amount of phosphorus (P) in the treated leaves was increased. However, the total phenolic content and accumulation of other classes of flavonoids such as hydroxycinnamic acids, flavonols, and dihydrochalcones were not influenced. Phostrade Ca treatment significantly increased dihydroflavonol 4-reductase (DFR) and slightly flavanone-3-hydroxylase (FHT) activity, which were correlated with anthocyanin synthesis but had no effect on phenylalanine ammonia lyase (PAL) and chalcone synthase/chalcone isomerase (CHS/CHI) activity. The results indicate that two foliar applications of Phostrade Ca late in the growing season represent an effective way to improve the color of ‘Braeburn’ apples at commercial harvest.

scholarly journals Metabolomic analysis reveals the interaction of primary and secondary metabolism in white, pale green, and green pak choi (Brassica rapa subsp. chinensis)

2021 ◽  
Vol 64 (1) ◽  
Author(s):  
Hyeon Ji Yeo ◽  
Seung-A Baek ◽  
Ramaraj Sathasivam ◽  
Jae Kwang Kim ◽  
Sang Un Park
Keyword(s):  
Secondary Metabolites ◽  
Brassica Rapa ◽  
High Performance ◽  
Reducing Power ◽  
Total Phenolic ◽  
Pak Choi ◽  
Primary And Secondary Metabolites ◽  
Chlorophyll Contents ◽  
Significant Difference ◽  
Pale Green

AbstractThis study aimed to comprehensively analyze primary and secondary metabolites of three different-colored (white, pale green, and green) pak choi cultivars (Brassica rapa subsp. chinensis) using gas chromatography attached with time-of-flight mass spectrometry (GC-TOFMS) and high-performance liquid chromatography (HPLC). In total, 53 primary metabolites were identified and subjected to partial least-squares discriminant analysis. The result revealed a significant difference in the primary and secondary metabolites between the three pak choi cultivars. In addition, 49 hydrophilic metabolites were detected in different cultivars. Total phenolic and glucosinolate contents were highest in the pale green and green cultivars, respectively, whereas total carotenoid and chlorophyll contents were highest in the white cultivar. Superoxide dismutase activity, 2,2-diphenyl-1-picrylhydraz scavenging, and reducing power were slightly increased in the white, pale green, and green cultivars, respectively. In addition, a negative correlation between pigments and phenylpropanoids was discovered by metabolite correlation analysis. This approach will provide useful information for the development of strategies to enhance the biosynthesis of phenolics, glucosinolates, carotenoids, and chlorophyll, and to improve antioxidant activity in pak choi cultivars. In addition, this study supports the use of HPLC and GC-TOFMS-based metabolite profiling to explore differences in pak choi cultivars.

DFR and PAL gene transcription and their correlation with anthocyanin accumulation in Rhodomyrtus tomentosa (Aiton.) Hassk.

2018 ◽  
Vol 44 (3) ◽  
pp. 289-298
Author(s):  
Bao-Jun Zhu ◽  
Qian Wang ◽  
Jing-Hui Wang ◽  
Lin-Lin Gao ◽  
Jing-Wen Zhang ◽  
...  
Keyword(s):  
Expression Patterns ◽  
Pcr Analysis ◽  
Anthocyanin Synthesis ◽  
Anthocyanin Content ◽  
Transcript Levels ◽  
Color Development ◽  
Phenylalanine Ammonialyase ◽  
Maturity Period ◽  
Late Maturity ◽  
Rhodomyrtus Tomentosa

Abstract Objectives Rhodomyrtus tomentosa (Aiton.) Hassk. (R. tomentosa) is rich in nutrients and has multiple pharmacological applications. Anthocyanins confer color to the flowers and berries of R. tomentosa and provide protection against photodamage. The dihydroflavonol 4-reductase gene (DFR) and phenylalanine ammonialyase gene (PAL) are crucial for anthocyanin synthesis. Methods DFR and PAL transcript levels and anthocyanin content in the pigmented organs of R. tomentosa were investigated through qRT-PCR analysis and spectrophotometry, respectively. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was selected as the reference gene for the normalization of DFR and PAL transcript levels. Results Transcript levels of DFR and PAL were higher in organs with vigorous metabolism than those in senescent organs. DFR and PAL transcript levels were up-regulated during the initial and middle-maturity periods of fruit. These expression patterns are consistent with fruit color development. The highest transcript levels of PAL and DFR were observed during the middle-maturity period or the red-fruit period. Conclusion During the late maturity period of R. tomentosa fruit, the transcript levels of the two genes were down-regulated even though anthocyanins were continuously accumulated, which was different from the accumulation of anthocyanins in some late mature fruits.

scholarly journals Characterization of Anthocyanins in Sweet Potato Leaves Grown in Various Stages and Conditions

2019 ◽  
pp. 253-262
Author(s):  
Xiaoyu Su ◽  
Zhenbao Jia ◽  
Fei Tao ◽  
Jiamin Shen ◽  
Jingwen Xu ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Anthocyanin Biosynthesis ◽  
Total Phenolic ◽  
Growth Stages ◽  
Anthocyanin Content ◽  
Potential Health ◽  
Significant Difference ◽  
Young Leaves ◽  
Sweet Potato Leaves

Phytochemical-enriched edible greens, sweet potato leaves (Ipomoea batatas L.), have become popular due to potential health benefits. However, the phytochemical contents in sweet potato leaves and their subsequent change over harvest stages and growth condition are mostly unknown. In this study, the anthocyanin profile and content in leaves of four sweet potato cultivars, i.e., white-skinned and white-fleshed Bonita, red-skinned and orange-fleshed Beauregard, red-skinned and white-fleshed Murasaki and purple-skinned and purple-fleshed P40, were evaluated. Fourteen anthocyanins were isolated and identified by HPLC-MSI/MS. The most abundant was cyanidin 3-caffeoyl-p-hydroxybenzoyl sophoroside-5-glucoside, which comprised up to 20% of the total anthocyanins. Of the young leaves (1st and 2nd slip cuttings), Bonita contained the highest anthocyanin content followed by P40. Of the mature leaves (vine stage), Beauregard had the greatest anthocyanin (592.5 ± 86.4 mg/kg DW) and total phenolic (52.2 ± 3 mg GAE/g DW). It should be noted that the lowest anthocyanin and total phenolic content of shoots were found in P40, while tubers of P40 contain the highest content of each. Furthermore, the increase in leaf anthocyanin content over the growth stages that was observed in three of the cultivars but not in P40. No significant difference of anthocyanin content was found in Beauregard leaves grown in the high tunnels when compared with that in the open field. This study demonstrated for the first time that anthocyanin levels were significantly changed in response to various growth stages but not high tunnel condition, indicating that the effect of anthocyanin biosynthesis in sweet potato leaves is highly variable and genotype specific.

scholarly journals The Content of Selected Minerals, Bioactive Compounds, and the Antioxidant Properties of the Flowers and Fruit of Selected Cultivars and Wildly Growing Plants of Sambucus nigra L.

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 876 ◽  
Author(s):  
Karolina Młynarczyk ◽  
Dorota Walkowiak-Tomczak ◽  
Halina Staniek ◽  
Marcin Kidoń ◽  
Grzegorz P. Łysiak
Keyword(s):  
Antioxidant Activity ◽  
Mineral Content ◽  
Antioxidant Properties ◽  
Absorption Spectrometry ◽  
Wild Plants ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Spectrophotometric Methods ◽  
Bioactive Properties ◽  
High Concentrations

This study compared the mineral content and bioactive properties of flowers and fruit coming from wild elderberry plants with those of flowers and fruit harvested from elderberry cultivars grown in an orchard. Elderberry fruit and flowers were analyzed for the content of selected minerals, phenolic compounds, and anthocyanins, as well as for antioxidant activity. Mineral content was determined by the atomic absorption spectrometry method, while antioxidant activity and the content of polyphenols and anthocyanins were determined by spectrophotometric methods. Flowers were found to contain more total ash and much higher content of most of minerals, except magnesium which was present in high concentrations in fruit. Fruit showed significantly higher antioxidant activity than flowers, whereas the total phenolic content varied depending on the growing location or cultivar. The material obtained from selected cultivars growing in an orchard had higher antioxidant activity and polyphenol and anthocyanin content than the material obtained from wild plants. Fruit of the ”Haschberg” cultivar and flowers of the ”Sampo” cultivar had the best bioactive properties of the studied samples.

Utilization of small broken riceberry flour in gluten-free bread

2019 ◽  
Vol 25 (6) ◽  
pp. 515-522
Author(s):  
Numfon Rakkhumkaew ◽  
Yuparat Boonsri ◽  
Arunwadee Sukchum
Keyword(s):  
Xanthan Gum ◽  
Sensory Quality ◽  
Amylose Content ◽  
Rice Flour ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Gluten Free ◽  
Total Anthocyanin ◽  
Total Anthocyanin Content ◽  
Significant Difference

The aim of this study was to develop gluten-free bread formulations based on small broken riceberry flour, by using different ratios of rice flour and xanthan gum. Small broken riceberry and rice flour could be classified as low in amylose content (15.70 g and 20.50 g/100 g dry matter for small broken riceberry and rice flour, respectively). Additionally, small broken riceberry flour contained a total phenolic and total anthocyanin content approximately 500 times higher than that of rice flour. The addition of increased amounts of small broken riceberry flour and xanthan gum resulted in darker coloured gluten-free bread. However, there was no significant difference regarding moisture and specific volume. The increase of small broken riceberry flour and xanthan gum also led to a significant increase in the firmness of bread crumbs. The sensory evaluation showed differences in flavour, texture and overall liking, since adding small broken riceberry flour tended to make gluten-free bread more favourable. Bread containing rice flour and small broken riceberry flour in the ratio of 30:70 and 1.0% xanthan gum was selected on the basis of the sensory quality. Moreover, such bread also contained high levels of total phenolic and anthocyanin content.

scholarly journals Comparative Transcriptome Analysis Reveals Effects of Exogenous Hematin on Anthocyanin Biosynthesis during Strawberry Fruit Ripening

2016 ◽  
Vol 2016 ◽  
pp. 1-14 ◽  
Author(s):  
Yi Li ◽  
Huayin Li ◽  
Fengde Wang ◽  
Jingjuan Li ◽  
Yihui Zhang ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Regulatory Networks ◽  
Anthocyanin Biosynthesis ◽  
Anthocyanin Synthesis ◽  
Control Group ◽  
Anthocyanin Content ◽  
Color Development ◽  
Strawberry Fruit Ripening ◽  
Positive Effect

Anthocyanin in strawberries has a positive effect on fruit coloration. In this study, the role of exogenous hematin on anthocyanin biosynthesis was investigated. Our result showed that the white stage of strawberries treated with exogenous hematin had higher anthocyanin content, compared to the control group. Among all treatments, 5 μM of hematin was the optimal condition to promote color development. In order to explore the molecular mechanism of fruit coloring regulated by hematin, transcriptomes in the hematin- and non-hematin-treated fruit were analyzed. A large number of differentially expressed genes (DEGs) were identified in regulating anthocyanin synthesis, including the DEGs involved in anthocyanin biosynthesis, hormone signaling transduction, phytochrome signaling, starch and sucrose degradation, and transcriptional pathways. These regulatory networks may play an important role in regulating the color process of strawberries treated with hematin. In summary, exogenous hematin could promote fruit coloring by increasing anthocyanin content in the white stage of strawberries. Furthermore, transcriptome analysis suggests that hematin-promoted fruit coloring occurs through multiple related metabolic pathways, which provides valuable information for regulating fruit color via anthocyanin biosynthesis in strawberries.

Effects of Vacuum Ohmic Evaporation on Some Quality Properties of Sour Cherry Juice Concentrates

2019 ◽  
Vol 15 (9) ◽  
Author(s):  
Serdal Sabancı ◽  
Filiz Icier
Keyword(s):  
Antioxidant Activities ◽  
Sour Cherry ◽  
Absolute Pressure ◽  
Total Phenolic ◽  
Voltage Gradient ◽  
Anthocyanin Content ◽  
Quality Properties ◽  
Significant Difference ◽  
Cherry Juice ◽  
Total Antioxidant

AbstractSour cherry juice (SJ) having total soluble solid (TSS) content of 19.2 % was concentrated to 65 % TSS by applying vacuum ohmic evaporation (VOE) and vacuum evaporation (VE) methods under constant absolute pressure (25 kPa). Total monomeric anthocyanin content (TMAC) of sour cherry concentrate (65 % TSS) was found in the range of 1561.67–1777.38 mg/L whereas total phenolic content (TPC) varied in the range of 9,071.22–78,347.53 mg/L concentrate. TPC and TMAC values of juice concentrates were affected less from VOE process compared to VE (p < 0.05) while there was no significant difference between the total antioxidant activities of SJ concentrates obtained by both methods (p > 0.05). TMAC and TPC values increased as the voltage gradient applied increased (p < 0.05). VOE process preserved the color properties of juice concentrates better compared to the VE process. It was concluded that VOE method could be an alternative fast evaporation method to obtain high-quality fruit juice concentrates.

scholarly journals Effect of Storage on the Nutritional Quality of Queen Garnet Plum

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 352
Author(s):  
Gethmini Kodagoda ◽  
Hung T. Hong ◽  
Tim J. O’Hare ◽  
Yasmina Sultanbawa ◽  
Bruce Topp ◽  
...  
Keyword(s):  
Nutritional Quality ◽  
Fold Increase ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Total Sugars ◽  
Storage Temperatures ◽  
Different Tissues

Due to high perishability, plums are harvested at an early stage of maturity to extend postharvest storage life. Storage time and temperature can significantly affect the phytochemical and sugar composition of plums, altering their palatability and nutritional quality. In this study, variations in physiochemical properties (total soluble solids (TSS), titratable acidity (TA), color (chroma and hue angle)), phytochemical composition (total phenolic content (TPC), total anthocyanin content (TAC), and carotenoids), and sugars in three different tissues of the Queen Garnet plum (QGP) during storage at two common domestic storage temperatures (4 and 23 °C) were evaluated. There was an increase (p > 0.05) in TSS and a reduction (p < 0.05) in TA of the outer flesh at 23 °C. Chroma values of all the tissues reduced (p < 0.05) at 23 °C. At 4 °C, chroma values fluctuated between storage days. The TAC of the peel was the highest (p < 0.05) among the different tissues and continued to increase up to 10 days of storage at 23 °C (3-fold increase). At 4 °C, the highest (p < 0.05) TAC (peel) was observed after 14 days of storage (1.2-fold increase). TPC showed similar results. The highest (p < 0.05) TPC was recorded in the peel after 10 days of storage at 23 °C (2.3-fold increase) and after 14 days of storage at 4 °C (1.3-fold increase), respectively. Total carotenoids in the flesh samples at both storage temperatures were reduced (p < 0.05) after 14 days. Total sugars also decreased during storage. The results of the present study clearly showed that common domestic storage conditions can improve the nutritional quality of plums by increasing the content of bioactive anthocyanins and other phenolic compounds. However, the increase in phytochemicals needs to be counterbalanced with the decrease in total sugars and TA potentially affecting the sensory attributes of the plums.

scholarly journals 197 Effect of STC-4771, An Intermediate of ABA Synthesis, on the Anthocyanin Accumulation in Grapes

HortScience ◽  
2000 ◽  
Vol 35 (3) ◽  
pp. 425A-425
Author(s):  
W.S. Lee ◽  
J.C. Lee ◽  
Y.S. Hwang
Keyword(s):  
Anthocyanin Synthesis ◽  
Anthocyanin Content ◽  
Internal Factors ◽  
External Application ◽  
Grape Berries ◽  
Color Development ◽  
Agricultural Use ◽  
Favorable Conditions

The coloration of grape berries depends on the anthocyanin synthesis during maturation. The quality of berries is often decreased due to the poor color development when berries are grown under unfavorable environments and/or inadequate internal factors are involved. It has been well-known that the level of ABA at ripening is closely associated with anthocyanin synthesis; thus, the external application of ABA results in the increase of anthocyanin content even in berries grown under favorable conditions. However, the agricultural use of natural ABA is not possible because of high prices. This experiment was conducted to study the potential of STC-4771 as a substitute for ABA. The effect of STC-4771 was studied in `Kyoho', `Pione', and `Delaware' grapes. Chemicals were applied when ≈10% of berries in a cluster were colored. In `Kyoho', anthocyanin synthesis was enhanced at a concentration of 100 mg/L and there was a trend in color enhancement in `Pione', regardless of treatment concentration, between 10 to 40 mg/L. However, no clear effect was found in `Delaware' at 50 to 100 mg/L. In an in vitro experiment, anthocyanin was only increased when an adequate amount of sucrose (0.6 m) was added in the incubation medium under light. Natural ABA effectively increased the anthocyanin content of berry segments even under shading condition through four bagging materials, but no effect was confirmed in STC treatment.

scholarly journals Differential Effects of Ethephon and Seniphos on Color Development of `Starking Delicious' Apple

1996 ◽  
Vol 121 (4) ◽  
pp. 746-750 ◽  
Author(s):  
C. Larrigaudiere ◽  
E. Pinto ◽  
M. Vendrell
Keyword(s):  
Phenylalanine Ammonia Lyase ◽  
Anthocyanin Biosynthesis ◽  
Soluble Solids ◽  
Anthocyanin Content ◽  
Fruit Firmness ◽  
Differential Effects ◽  
Color Development ◽  
Commercial Harvest ◽  
Peel Color ◽  
Determining Factor

The differential effects of two color improving products, ethephon an ethylene-releasing compound, and seniphos, a nonethylene-releasing product, were studied on `Starking Delicious' apples (Malus domestica Borkh L.). Ethephon and seniphos were applied 2 or 3 weeks before commercial harvest. Ethephon- and seniphos-treated fruit showed a significant improvement of peel color associated with a sharp increase in anthocyanin content and chromaticity values. Color improvement in ethephon-treated apples occurred during the preharvest period and cold storage. The seniphos-treated fruit stopped color development in cold conditions. In comparison to the ethephon-treated fruit, the seniphos-treated apples showed lower internal ethylene concentrations and a ripening delay. Both treatments sharply increased the activity of phenylalanine-ammonia-lyase enzyme, which seemed to be the determining factor of color enhancement. The seniphos-treated apples compared to ethephon had higher fruit firmness and lower soluble solids concentrations. Anthocyanin biosynthesis may be enhanced by seniphos treatment without inducing ethylene production or other ripening associated changes. As a consequence, fruit treated with seniphos can be held longer in storage.

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