Fasting and Refeeding Affect the Goose Liver Transcriptome Mainly Through the PPAR Signaling Pathway

AbstractDemethylase Tet2 plays a vital role in the immune response. Acute kidney injury (AKI) initiation and maintenance phases are marked by inflammatory responses and leukocyte recruitment in endothelial and tubular cell injury processes. However, the role of Tet2 in AKI is poorly defined. Our study determined the degree of renal tissue damage associated with Tet2 gene expression levels in a cisplatin-induced AKI mice model. Tet2-knockout (KO) mice with cisplatin treatment experienced severe tubular necrosis and dilatation, inflammation, and AKI markers’ expression levels than the wild-type mice. In addition, the administration of Tet2 plasmid protected Tet2-KO mice from cisplatin-induced nephrotoxicity, but not Tet2-catalytic-dead mutant. Tet2 KO was associated with a change in metabolic pathways like retinol, arachidonic acid, linolenic acid metabolism, and PPAR signaling pathway in the cisplatin-induced mice model. Tet2 expression is also downregulated in other AKI mice models and clinical samples. Thus, our results indicate that Tet2 has a renal protective effect during AKI by regulating metabolic and inflammatory responses through the PPAR signaling pathway.

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Ellagic Acid Activated PPAR Signaling Pathway to Protect Ileums Against Castor Oil-Induced Diarrhea in Mice: Application of Transcriptome Analysis in Drug Screening

Frontiers in Pharmacology ◽

10.3389/fphar.2019.01681 ◽

2020 ◽

Vol 10 ◽

Author(s):

Jianqing Chen ◽

Hongliang Yang ◽

Zunlai Sheng

Keyword(s):

Signaling Pathway ◽

Drug Screening ◽

Transcriptome Analysis ◽

Ellagic Acid ◽

Castor Oil ◽

Ppar Signaling

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Omics multi-layers networks and identification of genes involved in fat storage and metabolism in poultry using interactomics approach

10.21203/rs.3.rs-30697/v1 ◽

2020 ◽

Author(s):

abolfazl bahrami ◽

Farzad Ghafouri ◽

Mostafa Sadeghi ◽

Seyed Reza Miraei-Ashtiani

Keyword(s):

Adipose Tissue ◽

Signaling Pathway ◽

High Throughput Screening ◽

Broiler Chickens ◽

Unsaturated Fatty Acids ◽

Holistic Approach ◽

High Rate ◽

Literature Mining ◽

Ampk Signaling ◽

Ppar Signaling

Abstract Background Fatty acid metabolism in animals has a major impact on production and disease resistance traits. According to the high rate of interactions between lipid metabolism and its regulating properties, a holistic approach is necessary. Methods To study multi-omics layers of adipose tissue and identification of genes involved in fat metabolism, storage and endocrine signaling pathways in two groups of broiler chickens with high and low abdominal fat, high-throughput screening (HTS) techniques were used. The Gene-miRNA interacting bipartite and metabolic-signaling networks were reconstructed using their interactions. Results In the analysis of microarray and RNA-Seq data, 1835 genes were detected by comparing the identified genes with significant expression differences. Then, by comparing, 34 genes and 19 miRNAs were detected as common and main nodes. The literature mining approach was used and 7 genes were identified and added to the common gene set. Module finding revealed three important and functional modules. The detected modules 1, 2, and 3 were involved in the PPAR signaling pathway, biosynthesis of unsaturated fatty acids, and Alzheimer's disease metabolic pathway, adipocytokine, insulin, PI3K-Akt, mTOR and AMPK signaling pathway. Conclusions This approach revealed a new insight for a better understanding of the biological processes associated with adipose tissue.

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Identification of a Potential PPAR-Related Multigene Signature Predicting Prognosis of Patients with Hepatocellular Carcinoma

PPAR Research ◽

10.1155/2021/6642939 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Wenfang Xu ◽

Zhen Chen ◽

Gang Liu ◽

Yuping Dai ◽

Xuanfu Xu ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Signaling Pathway ◽

Target Genes ◽

Prognostic Markers ◽

Cox Regression ◽

Clinical Information ◽

Gene Signature ◽

Peroxisome Proliferator ◽

Clinical Indicators ◽

Ppar Signaling

Peroxisome proliferator-activated receptors (PPARs) and part of their target genes have been reported to be related to the progression of hepatocellular carcinoma (HCC). The prognosis of HCC is not optimistic, and more accurate prognostic markers are needed. This study focused on discovering potential prognostic markers from the PPAR-related gene set. The mRNA data and clinical information of HCC were collected from TCGA and GEO platforms. Univariate Cox and lasso Cox regression analyses were used to screen prognostic genes of HCC. Three genes (MMP1, HMGCS2, and SLC27A5) involved in the PPAR signaling pathway were selected as the prognostic signature of HCC. A formula was established based on the expression values and multivariate Cox regression coefficients of selected genes, that was, risk score = 0.1488 ∗ expression value of M M P 1 + − 0.0393 ∗ expression value of H M G C S 2 + − 0.0479 ∗ expression value of S L C 27 A 5 . The prognostic ability of the three-gene signature was assessed in the TCGA HCC dataset and verified in three GEO sets (GSE14520, GSE36376, and GSE76427). The results showed that the risk score based on our signature was a risk factor with a HR (hazard ratio) of 2.72 ( 95 % CI Confidence Interval = 1.87 ~ 3.95 , p < 0.001 ) for HCC survival. The signature could significantly ( p < 0.0001 ) distinguish high-risk and low-risk patients with poor prognosis for HCC. In addition, we further explored the independence and applicability of the signature with other clinical indicators through multivariate Cox analysis ( p < 0.001 ) and nomogram analysis ( C ‐ index = 0.709 ). The above results indicate that the combination of MMP1, HMGCS2, and SLC27A5 selected from the PPAR signaling pathway could effectively, independently, and applicatively predict the prognosis of HCC. Our research provided new insights to the prognosis of HCC.

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A Network Pharmacology Approach to Explore Mechanism of Action of Longzuan Tongbi Formula on Rheumatoid Arthritis

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2019/5191362 ◽

2019 ◽

Vol 2019 ◽

pp. 1-13 ◽

Cited By ~ 5

Author(s):

An Huang ◽

Gang Fang ◽

Yuzhou Pang ◽

Zongran Pang

Keyword(s):

Rheumatoid Arthritis ◽

Signaling Pathway ◽

Active Rheumatoid Arthritis ◽

Trp Channels ◽

Network Pharmacology ◽

Mtor Signaling Pathway ◽

Active Ingredients ◽

Major Pathway ◽

Pharmacological Mechanism ◽

Ppar Signaling

Longzuan Tongbi Formula (LZTB) is an effective proved prescription in Zhuang medicine for treating active rheumatoid arthritis (RA). However, its active ingredients, underlying targets, and pharmacological mechanism are still not clear in treating RA. We have applied network pharmacology to study LZTB and found that 8 herbs in LZTB and 67 compounds in the 8 herbs are involved in the regulation of RA-related genes; we have conducted pathway analysis of overlapping genes and found that 7 herbs participate in the regulations of 24 pathways associated with RA and that 5 herbs in the 7 herbs and 25 compounds in the 5 herbs participate in the regulation of hsa05323 (rheumatoid arthritis). The results indicated that all herbs in LZTB and some compounds in those herbs participate in the treatment of RA; 25 compounds are main active ingredients and hsa05323 (rheumatoid arthritis) is the major pathway in the treatment of RA. We have also found that three pathways (inflammatory mediator regulation of TRP channels, PPAR signaling pathway, and mTOR signaling pathway) might have some effect on the treatment of RA.

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Genetic variants of the peroxisome proliferator‐activated receptor (PPAR) signaling pathway genes and risk of pancreatic cancer

Molecular Carcinogenesis ◽

10.1002/mc.23208 ◽

2020 ◽

Vol 59 (8) ◽

pp. 930-939

Author(s):

Xiaowen Liu ◽

Danwen Qian ◽

Hongliang Liu ◽

James L. Abbruzzese ◽

Sheng Luo ◽

...

Keyword(s):

Pancreatic Cancer ◽

Signaling Pathway ◽

Genetic Variants ◽

Peroxisome Proliferator ◽

Peroxisome Proliferator Activated Receptor ◽

Ppar Signaling

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PPAR signaling pathway in the first trimester placenta from in vitro fertilization and embryo transfer

Biomedicine & Pharmacotherapy ◽

10.1016/j.biopha.2019.109251 ◽

2019 ◽

Vol 118 ◽

pp. 109251 ◽

Cited By ~ 2

Author(s):

Liang Zhao ◽

Xiuli Zheng ◽

Jingfang Liu ◽

Rong Zheng ◽

Rui Yang ◽

...

Keyword(s):

In Vitro Fertilization ◽

Signaling Pathway ◽

Embryo Transfer ◽

First Trimester ◽

Vitro Fertilization ◽

Ppar Signaling

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SP016DYSREGULATION OF PPAR SIGNALING PATHWAY, FOCAL ADHESION PATHWAY AND DECREASED INTEGRIN LINKED KINASE ARE EARLY EVENTS IN COL4A3 −/− MICE MANIFESTING ALPORT SYNDROME

Nephrology Dialysis Transplantation ◽

10.1093/ndt/gfx138.sp016 ◽

2017 ◽

Vol 32 (suppl_3) ◽

pp. iii107-iii107

Author(s):

Andreas Kousios ◽

Orthodoxia Nicolaou ◽

Paraskevi Chairta ◽

Andreas Hadjisavvas ◽

Kyriakos Ioannou ◽

...

Keyword(s):

Signaling Pathway ◽

Focal Adhesion ◽

Alport Syndrome ◽

Ppar Signaling ◽

Integrin Linked Kinase ◽

Focal Adhesion Pathway

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Downregulated developmental processes in the postnatal right ventricle under the influence of a volume overload

Cell Death Discovery ◽

10.1038/s41420-021-00593-y ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Chunxia Zhou ◽

Sijuan Sun ◽

Mengyu Hu ◽

Yingying Xiao ◽

Xiafeng Yu ◽

...

Keyword(s):

Cell Cycle ◽

Right Ventricle ◽

Signaling Pathway ◽

Sham Group ◽

Vascular Endothelial Cells ◽

Vena Cava ◽

Volume Overload ◽

Cardiac Regeneration ◽

Mitotic Cell ◽

Ppar Signaling

AbstractThe molecular atlas of postnatal mouse ventricular development has been made available and cardiac regeneration is documented to be a downregulated process. The right ventricle (RV) differs from the left ventricle. How volume overload (VO), a common pathologic state in children with congenital heart disease, affects the downregulated processes of the RV is currently unclear. We created a fistula between the abdominal aorta and inferior vena cava on postnatal day 7 (P7) using a mouse model to induce a prepubertal RV VO. RNAseq analysis of RV (from postnatal day 14 to 21) demonstrated that angiogenesis was the most enriched gene ontology (GO) term in both the sham and VO groups. Regulation of the mitotic cell cycle was the second-most enriched GO term in the VO group but it was not in the list of enriched GO terms in the sham group. In addition, the number of Ki67-positive cardiomyocytes increased approximately 20-fold in the VO group compared to the sham group. The intensity of the vascular endothelial cells also changed dramatically over time in both groups. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of the downregulated transcriptome revealed that the peroxisome proliferators-activated receptor (PPAR) signaling pathway was replaced by the cell cycle in the top-20 enriched KEGG terms because of the VO. Angiogenesis was one of the primary downregulated processes in postnatal RV development, and the cell cycle was reactivated under the influence of VO. The mechanism underlying the effects we observed may be associated with the replacement of the PPAR-signaling pathway with the cell-cycle pathway.

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