scholarly journals Effect of tear/crack on soybean (Glycine max) seed coat, physiological quality and pathology of the seed

2019 ◽  
pp. 988-994 ◽  
Author(s):  
Bruna Ribeiro Machado ◽  
Caio Cesar de Oliveira Pereira ◽  
Élida Priscila Bogéa Carvalho ◽  
Juliana Oliveira da Silva ◽  
Letícia Maria de Oliveira Mendes ◽  
...  
Keyword(s):  
Glycine Max ◽  
Seed Coat ◽  
Seed Quality ◽  
Soybean Seed ◽  
Storage Period ◽  
Moisture Damage ◽  
Pathological Effect ◽  
Randomized Design ◽  
Physiological Quality ◽  
The Impact

Seed coat tear is frequently occurs in some soybean cultivars. The impact of seed coat tear on seed physiology and sanitation is uncertain. Our objective was to analyze the physiological and pathological effect of tear on soybean (Glycine max) seed coat. The cultivars NS-8338-IPRO (with high incidence of tear) and NS-6906-IPRO (with low incidence of tear) were used. A tetrazolium test was used to assess the physiological quality of the seed coat before and after storage. The sanitary quality was assessed through the "Blotter Test". For each storage period, we evaluated seeds with 0% coat tear, up to 10%, and above 10%. The experiment was performed in a completely randomized design using the methodologies proposed by RAS. The coat tear on soybean seed positively contributed to the moisture damage affecting the inner part of the seed. Incidences of Fusarium sp. and Aspergillus sp. were frequently observed in torn seeds and in seeds without tear (around 9-10%) but did not interfere with seed quality. The appearance of coat tear on soybean seed is increased by moisture damage and do not serve as a gateway for the fungi to cause damage during seed emergence.

scholarly journals Influence of lignin content in soybean seed coat on the incidence of the storage fungus Aspergillus flavus

2012 ◽  
Vol 34 (4) ◽  
pp. 541-548 ◽  
Author(s):  
Ísis Barreto Dantas ◽  
João Almir de Oliveira ◽  
Heloisa Oliveira dos Santos ◽  
Édila Vilela Resende Von Pinho ◽  
Sttela Dellyzete Veiga Franco da Rosa
Keyword(s):  
Electrical Conductivity ◽  
Aspergillus Flavus ◽  
Seed Coat ◽  
Seed Quality ◽  
Lignin Content ◽  
Soybean Seed ◽  
Storage Period ◽  
Seed Storage ◽  
Seed Health ◽  
Physiological Quality

Seed quality may be affected by several factors, including permeability, color, and lignin content in the seed coat. This study aimed at evaluating influence of lignin content in the tegument of seed samples of six different soybean cultivars, in which half of each sample was inoculated with the fungus Aspergillus flavus, on the physical and physiological quality, and on the seed health, during 180 days storage period, under cold chamber with controlled conditions of temperature and RH. For that, at each interval of 60 days, samples were removed, and the physiological quality of these seeds was assessed by means of moisture and lignin contents; and by tests of seed health, germination, and electrical conductivity. The moisture content of seeds remained constant during all storage period. In the seed health test, it was found that inoculation was efficient, once the minimum incidence of the fungus in the inoculated seeds was 85%. In the germination test, there was a trend of reduction on percentage germination with the increase in storage period. However, there was an increase on electrical conductivity of seeds assessed. It was concluded that there is no interference of the lignin content in the seed coat on the resistance to infection by the fungus Aspergillus flavus, even after seed storage for a period of 180 days.

scholarly journals Seed quality of rice cultivars stored in different environments

2014 ◽  
Vol 36 (1) ◽  
pp. 32-39 ◽  
Author(s):  
Elizabeth Rosemeire Marques ◽  
Eduardo Fontes Araújo ◽  
Roberto Fontes Araújo ◽  
Sebastião Martins Filho ◽  
Plínio César Soares
Keyword(s):  
Seed Quality ◽  
Accelerated Aging ◽  
Storage Period ◽  
Rice Cultivars ◽  
Germination Test ◽  
Randomized Design ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
And Storage

The objective of this study was to evaluate dormancy and physiological quality of seeds of rice cultivars during storage in different environments. After harvesting, the seeds of three rice cultivars (Seleta, Curinga and Relâmpago) were dried in the sun, to reach moisture content at around 13%. Then, they were packed in paper and stored in four environments: 5 ± 2 ºC / 70 ± 5% RH, 12 ± 2 ºC / 70 ± 5% RH, 18 ± 2 ºC / 65 ± 5% RH and in uncontrolled condition of temperature and relative humidity (natural). Physiological quality was evaluated at the beginning and at 3, 6, 9 and 12 months of storage by germination test, electrical conductivity, accelerated aging and sand emergence. The experiment was conducted in split plots in a completely randomized design with three replications. Environmental factor was applied in the plots, cultivars in the subplots and storage period in subplots. Cultivar Seleta showed higher dormancy, which was surpassed during storage regardless of environment conservation. In general, seeds stored in natural environment showed lower physiological quality. Only the seeds of cultivar Seleta, regardless of the environment, maintained germination above the minimum required for commercialization until six months of storage.

scholarly journals Ragam Karakter Morfologi Kulit Biji Beberapa Genotipe Plasma Nutfah Kedelai

2016 ◽  
Vol 14 (1) ◽  
pp. 14
Author(s):  
Ayda Krisnawati ◽  
M. M. Adie
Keyword(s):  
Cluster Analysis ◽  
Seed Coat ◽  
Seed Quality ◽  
Soybean Seed ◽  
Morphological Characters ◽  
Soybean Germplasm ◽  
Randomized Design ◽  
Soybean Genotypes ◽  
Soybean Seed Coat ◽  
Coat Layer

<p>Identification of morphological characters of soybean seed coat can be beneficial for industrial purposes and also important to enhance the seed quality genetically. The aims of the experiment were to identify the seed coat morphology of several soybean germplasm and to group them based on their seed coat morphological character. A total of 25 Balitkabi’s soybean germplasm were identified in Laboratory of Plant Breeding, Balitkabi and Laboratory of Biology, Malang University, from June until August 2005. The completely randomized design was used with three replications. Parameter measured include the thickness of seed coat layer and rate of imbibition. Cluster analysis was used to determine the index similarity of 25 soybean germplasms based on seed coat characters included epidermis, hypodermis, and parenchyma. The results shows that epidermis, hypodermis and parenchyma layer have mean of 0.051 mm, 0.160 mm, and 0.152 m, respectively. Imbibition’s rate ranged from 0.29-0.54% per hour. Cluster analysis was successfully clustering soybean genotypes into five groups, i.e. group with thin hypodermis layer, thin parenchyma, thick parenchyma, thick epidermis and hypodermis, and thinner parenchyma.</p><p> </p><p><strong>Abstrak</strong></p><p>Identifikasi karakter morfologi kulit biji kedelai tidak hanya bermanfaat untuk industri, namun juga penting untuk peningkatan mutu benih secara genetik. Tujuan penelitian adalah mengidentifikasi morfologi kulit biji beberapa genotipe plasma nutfah kedelai dan mengelompokkan berdasarkan karakter morfologi kulit biji. Bahan penelitian adalah 25 genotipe plasma nutfah kedelai yang dikoleksi oleh Balitkabi. Penelitian dilaksanakan di Laboratorium Pemuliaan Balitkabi dan Laboratorium Biologi FMIPA Universitas Negeri Malang, pada bulan Juni-Agustus 2005. Penelitian menggunakan rancangan acak lengkap, dengan tiga ulangan. Pengamatan dilakukan terhadap ketebalan lapisan epidermis, hipodermis, parenkim, dan laju imbibisi. Sidik gerombol digunakan untuk menilai derajat kemiripan 25 genotipe kedelai berdasarkan karakter kulit biji (epidermis, hipodermis, dan parenkim). Hasil penelitian menunjukkan bahwa lapisan epidermis, hipodermis, dan parenkim 25 genotipe kedelai masing-masing mempunyai ketebalan ratarata 0,051 mm, 0,160 mm, dan 0,152 mm. Kecepatan imbibisi berkisar antara 0,29-0,54%/jam. Sidik gerombol berhasil mengelompokkan 25 genotipe kedelai menjadi lima kelompok, yaitu kelompok genotipe yang memiliki lapisan hipodermis tipis, lapisan parenkim tipis, lapisan parenkim tebal, lapisan epidermis, hipodermis tebal, dan genotipe dengan lapisan parenkim yang lebih tipis.</p>

scholarly journals Effect of polyethylene glycol 6000 and storage period on seed quality of cocoa (Theobroma cacao L.)

2014 ◽  
Vol 30 (1) ◽  
Author(s):  
Astiti Rahayu ◽  
Triani Hardiyati ◽  
Ponendi Hidayat
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Storage Period ◽  
Seed Storage ◽  
Peg 6000 ◽  
Randomized Design ◽  
Long Time ◽  
Physiological Quality ◽  
And Storage

Increased productivity of cocoa needs high quality of cocoa seeds which are generally provided by certified seed gardens located far from smallholders farm, where seed delivery takes long time and may reduce physiological quality of seeds. One effort to maintain the seed quality is by treatment the seeds with polyethylene glycol6000 (PEG 6000). This study was aimed to study the interaction of PEG 6000 concentration and storage period on cocoa seed quality, and to determine the best concentration of PEG 6000 and storage period to maintain cocoa seed quality. The research was conducted in seed storage room, seed laboratory and green houseof PPPPTK, Cianjur. The experimental design used was a factorial completely randomized design and each combination treatment repeated three times. The first factor was concentration of PEG 6000 of 0%, 20%, 40%, and 60%, and the second factor was the storage period of 3 weeks, 6 weeks, 9 weeks, and 12 weeks. The results showed that concentration of PEG 60% with three weeks storage period was able to minimize the number of moldy seeds. The concentration of PEG 20%, 40%, and 60% were able to prevent seed germination in storage until six weeks. Concentration of PEG 6000 20% was able to maintain moisture content, seed germination, and germination rate.Key words:cocoa seed, storage, recalcitrant, PEG

Mineral concentrations in soybean seed produced under high day and night temperature

2001 ◽  
Vol 81 (4) ◽  
pp. 595-600 ◽  
Author(s):  
L. R. Gibson ◽  
R. E. Mullen
Keyword(s):  
Glycine Max ◽  
High Temperature ◽  
Mineral Composition ◽  
Seed Size ◽  
Seed Quality ◽  
Soybean Seed ◽  
Growth Period ◽  
Reproductive Growth ◽  
Mineral Concentrations ◽  
The Impact

Environmental stress during reproductive growth can alter the mineral composition of soybean [Glycine max (L.) Merr.] seed, which can affect nutritional value, functional quality, and seed quality. This study was conducted to determine the impact of different combinations of day and night temperatures during reproductive growth on the final mineral concentrations in soybean seed. Day/night temperatures of 30/20, 30/30, 35/20, and 35/30°C were imposed during flowering and pod set (R1 to R5), seed fill and maturation (R5 to R8), and during the entire reproductive growth period (R1 to R8). The soybean seed was harvested at maturity and analyzed for elemental concentration of P, K, Ca, Mg, Mn, Fe, B, Cu, Zn, Al, and Na. Calcium and Mn declined and Na increased as mean day temperature during R1-R5 increased from 30 to 35°C. Phosphorus, K, Ca, and Mg became more concentrated in the seed with increased temperature during R5 to R8. The concentration of several minerals was increased by temperature treatments during R1 to R8. The exceptions were Mg and Fe, which declined, and Mn and Al, which were unchanged. Phosphorus, K, Fe, Cu, and Zn concentrations were negatively correlated with seed size. Concentrations of these minerals in seed may be greater in soybean grown in high temperature environments that limit seed size. The changes in mineral concentrations resulting from high temperature may alter soybean nutritional quality and modify its end-use properties for oil processing and tofu production. Key words: Soybean, Glycine max, mineral composition, temperature

scholarly journals Physiological and sanitary quality of soybean seeds under different chemical treatments during storage

2016 ◽  
Vol 20 (11) ◽  
pp. 1020-1024 ◽  
Author(s):  
Gerusa M. Conceição ◽  
Alessandro D. Lúcio ◽  
Liliane M. Mertz-Henning ◽  
Fernando A. Henning ◽  
Manoela Beche ◽  
...  
Keyword(s):  
Chemical Treatment ◽  
Seed Quality ◽  
Accelerated Aging ◽  
Storage Period ◽  
Dry Weight ◽  
Soybean Seeds ◽  
Randomized Design ◽  
Seedling Length ◽  
Physiological Quality

ABSTRACT The purpose of this study was to evaluate the effect of the chemical treatment with fungicide, insecticide, micronutrient and polymer on physiological and sanitary quality of soybean seeds during storage. The treatments were arranged in a 3 x 5 x 5 factorial scheme (cultivars x seeds treatment x storage period), in completely randomized design with four replicates. Three cultivars were used: NA 4823RG, BMX TurboRR and Fundacep 62RR. The treatments were: T1: no chemical treatment, T2: fungicide, insecticide and micronutrient; T3: fungicide, insecticide, micronutrient and polymer, T4: fungicide; T5: insecticide. After the chemical treatment, the seeds were stored under environmental conditions from May to December 2012, and seed quality was evaluated at 0, 2, 4, 6 and 8 months of storage. Seeds water content and physiological quality were determined through tests of germination, accelerated aging, seedling length, seedling dry weight and sanity. The treatment with fungicides, insecticides, micronutrients and polymer did not affect seed quality over eight months of storage and promoted the control fungi associated with the seeds.

scholarly journals Genetic diversity in soybean seed quality under different storage conditions

2017 ◽  
Vol 38 (1) ◽  
pp. 57
Author(s):  
Daniele Piano Rosa ◽  
Danúbia Aparecida Costa Nobre ◽  
Diego Santos Oliveira ◽  
Francisco Charles dos Santos Silva ◽  
André Ricardo Gomes Bezerra ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Seed Quality ◽  
Soybean Seed ◽  
High Standard ◽  
Storage Conditions ◽  
Seed Vigor ◽  
Soybean Cultivars ◽  
High Germination ◽  
Physiological Quality ◽  
Almost All

This study aimed to assess the effect of genetic diversity on physiological quality of soybean seeds stored in cold chamber and under environmental conditions. Ten cultivars were assessed in a randomized factorial design (2x10). Factor 1 corresponded to two storage conditions and factor 2 to ten soybean cultivars, with four replications. The evaluated variables were total germination (G%), first count of germination (F%), percentage of abnormal seedlings (AS%), germination speed index (GSI), water content (WC), electrical conductivity (EC), dry matter of seedlings (DMS) and length of seedlings (LS). Data underwent ANOVA, followed by Scott Knott test, as well as multivariate analysis of genetic diversity. The results showed a higher physiological quality for seeds under cold storage. Half of the cultivars (FPS Júpiter, FPS Urano, FPS Antares, FPS Netuno and CD 250) presented high germination rates and seed vigor, being thus indicated as high-standard materials for further breeding programs. Besides that, storage environment had influence on the clustering of soybean cultivars. Moreover, cultivars had genetic dissimilarity for almost all assessed traits as G%, GSI, F%, AS%, EC, DMS and LS.

scholarly journals Alternative procedure for the cold test for soybean seeds

2010 ◽  
Vol 67 (5) ◽  
pp. 540-545 ◽  
Author(s):  
Bruno Guilherme Torres Licursi Vieira ◽  
Roberval Daiton Vieira ◽  
Francisco Carlos Krzyzanowski ◽  
José de Barros França Neto
Keyword(s):  
Glycine Max ◽  
Seed Quality ◽  
Soybean Seed ◽  
Cold Test ◽  
Seed Vigor ◽  
Alternative Procedure ◽  
Test Procedures ◽  
Paper Towel ◽  
Traditional Procedure ◽  
Seed Crops

The growing demand for high quality soybean [Glycine max (L.) Merrill] seeds requires a precise seed quality control system from the seed industry. One way to accomplish this is by improving vigor testing. Cold test has been traditionally employed for corn seeds. However, it has also been used for other seed crops such as cotton (Gossypium spp.), soybean (Glycine Max), dry bean (Phaseolus vulgaris) and pea (Pisum sativum). This study was carried out with the objective of adjusting an alternative procedure for the cold test to determine soybean seed vigor. Six commercial soybean seed lots of the cultivar BRS 133 were used. The physiological potential of the seed lots was evaluated by germination on paper towel and sand box, seedling field emergence, tetrazolium, accelerated aging and electrical conductivity tests. Seed moisture content was also determined. The temperature used for the cold test procedures was 10ºC during five days. Four cold test procedures were evaluated: i) plastic boxes with soil; ii) rolled paper towel with soil; iii) rolled paper towel without soil, and iv) an alternative procedure, using rolled paper towel without soil under cold water. A completely randomized experimental design with eight replications was used and the means were compared by the Tukey test (p = 0.05). To verify the dependence between the alternative test and others single linear correlation was used. All cold test procedures had similar coefficients of variation (CV), highlighting that rolled paper towel with soil and the alternative procedure had the best performance, with an average of 94% and 93% normal seedlings and CV of 3.2% and 3.6%, respectively. The alternative procedure has satisfactory results for estimating soybean seed vigor, yielding consistent results compared to the traditional procedure.

scholarly journals Selection of GmSWEET39 for oil and protein improvement in soybean

PLoS Genetics ◽  
2020 ◽  
Vol 16 (11) ◽  
pp. e1009114
Author(s):  
Hengyou Zhang ◽  
Wolfgang Goettel ◽  
Qijian Song ◽  
He Jiang ◽  
Zhenbin Hu ◽  
...  
Keyword(s):  
Seed Coat ◽  
Molecular Basis ◽  
Seed Quality ◽  
Soybean Protein ◽  
Wild Soybean ◽  
Soybean Seed ◽  
Pleiotropic Effect ◽  
Industrial Applications ◽  
Dual Function ◽  
Protein Accumulation

Soybean [Glycine max (L.) Merr.] was domesticated from wild soybean (G. soja Sieb. and Zucc.) and has been further improved as a dual-use seed crop to provide highly valuable oil and protein for food, feed, and industrial applications. However, the underlying genetic and molecular basis remains less understood. Having combined high-confidence bi-parental linkage mapping with high-resolution association analysis based on 631 whole sequenced genomes, we mapped major soybean protein and oil QTLs on chromosome15 to a sugar transporter gene (GmSWEET39). A two-nucleotide CC deletion truncating C-terminus of GmSWEET39 was strongly associated with high seed oil and low seed protein, suggesting its pleiotropic effect on protein and oil content. GmSWEET39 was predominantly expressed in parenchyma and integument of the seed coat, and likely regulates oil and protein accumulation by affecting sugar delivery from maternal seed coat to the filial embryo. We demonstrated that GmSWEET39 has a dual function for both oil and protein improvement and undergoes two different paths of artificial selection. A CC deletion (CC-) haplotype H1 has been intensively selected during domestication and extensively used in soybean improvement worldwide. H1 is fixed in North American soybean cultivars. The protein-favored (CC+) haplotype H3 still undergoes ongoing selection, reflecting its sustainable role for soybean protein improvement. The comprehensive knowledge on the molecular basis underlying the major QTL and GmSWEET39 haplotypes associated with soybean improvement would be valuable to design new strategies for soybean seed quality improvement using molecular breeding and biotechnological approaches.

scholarly journals Soybean seed origin effects on physiological and sanitary quality and crop yield

2019 ◽  
Vol 40 (5) ◽  
pp. 1789 ◽  
Author(s):  
Eduardo Silvestrini Tonello ◽  
Nataliê Luíse Fabbian ◽  
Deivid Sacon ◽  
Aline Netto ◽  
Vanessa Neumann Silva ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Seed Quality ◽  
Soybean Seed ◽  
Seed Vigor ◽  
Production Costs ◽  
Crop Season ◽  
Sowing Time ◽  
Randomized Design ◽  
Significant Difference ◽  
Seed Origin

The suitable establishment of a crop depends on seed quality, among another factors. However, with high production costs many producers use uncertified seeds, to reduce expenses with this input at sowing time. The objective was to evaluate germination, vigor and health of soybean seeds, diseases incidence in cvs. NS 5445 IPRO and BMX Ativa RR, whose seeds were of certified and uncertified origins, as well as yield components and grain yield of soybeans, with or without fungicides application. The experiments were conducted in the 2015/16 and 2016/17 crop season, in Erechim-RS. Two experiments were carried out in a completely randomized design (DIC): one in the laboratory, under a 2 x 2 factorial scheme (cultivar x origin); and another in the field in a homogeneous area, in a 2 x 2 factorial scheme (with/without fungicide application and certified/uncertified seed) for two cultivars, both with four replications. The variables evaluated were: germination and seed health, yield (kg ha-1), thousand grains weight (g), number of grains per plant, and incidence of foliar fungal diseases. The main fungi identified in seeds were Aspergillus sp., Penicillium sp. and Fusarium sp. The highest incidence percentage were obtained in uncertifed seeds, at two harvests. Both cultivars and origins presented the minimum germination required for commercialization, however, certified seeds had better performance for seed vigor. As for normal seedlings, there was a significant difference only for the 2016/17 crop season, with the highest percentage obtained in certified seeds (52.0% for NS 5445 and 73.5% for BMX Ativa). Best productivity was achieved with cultivation of certified seeds associated with fungicides in both crops and cultivars. For thousand grains weight (TGW) there was no difference in origin, but only for cultivar and fungicides apply. The number of grains per plant was higher in plants from certified seeds and that received fungicides, being cv. BMX Ativa the most responsive for the two harvests analyzed. The main diseases found in the two harvests were: Asian rust, powdery mildew, mildew, septoriosis and cercosporiosis. However, seed origin, in both crops, did not differ in the incidence of Asian rust, powdery mildew and mildew, but for septoriosis and cercosporiosis, considered soybean late season diseases, seed origin is a determining fator.

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