scholarly journals Recovery of microorganisms on fabric materials after low water temperature washing with non-oxidizing acidic bleaching formulation by culture method

2014 ◽  
Vol 12 (3) ◽  
pp. 418-425
Author(s):  
Jaesung Lee ◽  
John A. Lopes ◽  
Melvin A. Pascall
Keyword(s):  
Escherichia Coli ◽  
Water Temperature ◽  
Culture Method ◽  
Listeria Innocua ◽  
Cross Contamination ◽  
Food Grade ◽  
Washing Process ◽  
Fabric Materials ◽  
Washing Water ◽  
Blend Fabric

The recovery of microorganisms to different fabrics was evaluated after a washing process combined with a food-grade non-oxidizing acidic formulation and low washing water temperature. Cotton, polyester and a polyester/cotton blend fabric samples were inoculated with Escherichia coli, Listeria innocua and Saccharomyces cerevisiae, then dried for 1 day. They were separately placed in a simulated fabric washer and decontaminated for 1 and 10 min with the acidic formulation at 23 °C water washing temperature. The combination of direct detecting and dilution methods was used to detect survivors on fabrics. The use of ≥0.1% acidic formulation in the washing process significantly increased the efficacy of the washing for all fabric samples. Microorganisms on the cotton and mixed fabric appeared to bind more strongly and were more resistant to the washing process. No viability was observed on the fabric swatches at 1 cfu/sample detection limit when the washing process was combined with 0.5% acidic formulation in the 10 min washing cycle. These findings can be used to increase the efficiency of sanitizing fabrics in an environmentally friendly way, for remove harmful microorganisms from them and reduce cross-contamination.

Behavior of Shiga Toxigenic Escherichia coli Relevant to Lettuce Washing Processes and Consideration of Factors for Evaluating Washing Process Surrogates

2014 ◽  
Vol 77 (11) ◽  
pp. 1860-1867 ◽  
Author(s):  
KAIPING DENG ◽  
XUE WANG ◽  
LI-HAN YEN ◽  
HONGLIU DING ◽  
MARY LOU TORTORELLO
Keyword(s):  
Escherichia Coli ◽  
Bacterial Species ◽  
Wash Water ◽  
Lag Phase ◽  
Phenotypic Traits ◽  
Cross Contamination ◽  
Process Validation ◽  
E Coli ◽  
Hazard Characterization ◽  
Washing Process

Postharvest processes for fresh produce commonly include washing in water containing antimicrobial chemicals, such as chlorine; however, if the antimicrobials are not present in sufficient levels, washing can promote the spread of contamination that might be present. To understand cross-contamination risk during washing, we tested a collection of Shiga toxigenic Escherichia coli (STEC), including O157:H7 and other non-O157 strains, for certain traits during washing of fresh-cut lettuce, i.e., sensitivity to sublethal chlorine levels and ability to cross-contaminate (detach from and attach to) lettuce in the presence of sublethal chlorine levels. Nonpathogenic E. coli Nissle 1917 (EcN) and Pediococcus pentosaceus lactic acid bacterial species (LAB) were included as potential washing process validation surrogates. As measured by extension of the lag phase of growth in media containing 0.15 ppm of chlorine, chlorine sensitivity varied among the STECs. Cross-contamination was assessed by evaluating transfer of bacteria from inoculated to uninoculated leaves during washing. Without chlorine, similar transfer to wash water and uninoculated leaves was shown. In 1 ppm of chlorine, cross-contamination was not detected with most strains, except for the substantial transfer by a STEC O111 strain and EcN in some replicates. Strain O111 and EcN showed less inactivation in 0.25 ppm of chlorine water compared with O157 (P < 0.05). LAB showed similar transfer and similar chlorine inactivation to O157. Considering together the sublethal chlorine sensitivity and detachment/attachment traits, neither EcN nor LAB displayed optimal characteristics as washing process surrogates for the STEC strains, although further evaluation is needed. This work demonstrated a range of behaviors of STEC strains during lettuce washing and may be helpful in hazard characterization, identifying factors to consider for evaluating washing process efficacy, and identifying phenotypic traits to select surrogates to validate washing processes.

scholarly journals Impact of On-Farm Interventions against CTX-Resistant Escherichia coli on the Contamination of Carcasses before and during an Experimental Slaughter

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 228
Author(s):  
Michaela Projahn ◽  
Jana Sachsenroeder ◽  
Guido Correia-Carreira ◽  
Evelyne Becker ◽  
Annett Martin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Broiler Chickens ◽  
Stocking Density ◽  
Resistant Bacteria ◽  
Control Group ◽  
Cross Contamination ◽  
High Prevalence ◽  
On Farm ◽  
The Impact ◽  
Slow Growing

Cefotaxime (CTX)-resistant Enterobacteriaceae are still an ongoing challenge in human and veterinary health. High prevalence of these resistant bacteria is detected in broiler chickens and the prevention of their dissemination along the production pyramid is of major concern. The impact of certain on-farm interventions on the external bacterial contamination of broiler chickens, as well as their influence on single processing steps and (cross-) contamination, have not yet been evaluated. Therefore, we investigated breast skin swab samples of broiler chickens before and during slaughter at an experimental slaughter facility. Broiler chickens were previously challenged with CTX-resistant Escherichia coli strains in a seeder-bird model and subjected to none (control group (CG)) or four different on-farm interventions: drinking water supplementation based on organic acids (DW), slow growing breed Rowan × Ranger (RR), reduced stocking density (25 kg/sqm) and competitive exclusion with Enterobacteriales strain IHIT36098(CE). Chickens of RR, 25 kg/sqm, and CE showed significant reductions of the external contamination compared to CG. The evaluation of a visual scoring system indicated that wet and dirty broiler chickens are more likely a vehicle for the dissemination of CTX-resistant and total Enterobacteriaceae into the slaughterhouses and contribute to higher rates of (cross-) contamination during processing.

Growth of Selected Cross-Contaminating Bacterial Pathogens on Beef and Fish at 15 and 35°C+

1994 ◽  
Vol 57 (4) ◽  
pp. 337-340 ◽  
Author(s):  
AJIBOLA O. FAPOHUNDA ◽  
KENNETH W. MCMILLIN ◽  
DOUGLAS L. MARSHALL ◽  
W. M. WAITES
Keyword(s):  
Escherichia Coli ◽  
Clostridium Perfringens ◽  
Aeromonas Hydrophila ◽  
Growth Response ◽  
Cross Contamination ◽  
Limited Growth ◽  
E Coli ◽  
Fish Samples ◽  
Conditioning Temperature ◽  
The Tropics

Isolates of Escherichia coli and Clostridium perfringens from beef and Aeromonas hydrophila from fish were examined for their ability to survive and grow as cross-contaminates on nonnative tissues at simulated ambient (35°C) and aging/conditioning (15°C) temperatures of handling and retailing found in the tropics. Growth of all isolates over a 10-h period was greater (P < 0.05) on their native tissues at both temperatures. The aging/conditioning temperature effectively limited growth of E. coli and A. hydrophila to less than l-logl0 CFU/g and prevented growth of C. perfringens on beef and fish samples. All three isolates demonstrated characteristic mesophilic growth response on both tissues at 35°C during the 10-h retail period. The study suggests that two muscle food products could be jointly handled to efficiently use available storage/haulage capacity in tropical countries. Potential savings in space, labor and energy would be made if cross-contamination between the two products is minimized by available packaging and sanitizing technologies.

scholarly journals Nanopartículas de prata: método alternativo de sanitização para couve minimamente processada1

2015 ◽  
Vol 45 (2) ◽  
pp. 138-145 ◽  
Author(s):  
Emiliane Andrade Araújo ◽  
Flávia Regina Passos ◽  
Lorena Ribeiro ◽  
Adayane Aparecida Pereira ◽  
José Felício Queiroz Fialho Júnior
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Listeria Innocua

RESUMO O processamento mínimo de hortaliças visa, basicamente, a estender a vida útil dos alimentos, o que depende de uma série de fatores, como, por exemplo, a sanitização, a qual é considerada etapa fundamental para a qualidade microbiológica do alimento. No entanto, os agentes antimicrobianos usuais reduzem a população microbiana em, no máximo, dois ciclos logarítmicos. Dessa forma, existe a necessidade de desenvolvimento de sanitizantes alternativos. O presente trabalho objetivou aumentar a inocuidade de couve minimamente processada, por meio de sanitização com nanopartículas de prata. Observou-se que as nanopartículas promoveram três reduções logarítmicas, ou seja, 99,9 % de redução, na população de Escherichia coli inoculada na superfície da couve. Quando comparadas com outros agentes antimicrobianos (dicloroisocianurato de sódio e hipoclorito de sódio), as nanopartículas mostraram-se mais eficientes na sanitização da couve minimamente processada, apresentando menor contagem de mesófilos aeróbios. Observou-se, também, que a superfície da couve apresenta características hidrofóbicas, resultando em maior propensão para adesão bacteriana, o que foi confirmado na avaliação da termodinâmica de adesão favorável para Staphylococcus aureus, Escherichia coli e Listeria innocua.

scholarly journals The Utilization of Rice Wastewater Combined With Red Dragon Fruit (Hylocereus polyrhizus) as Basic Ingredients of Nata De Leri

2019 ◽  
Vol 6 (1) ◽  
pp. 62-69
Author(s):  
Meri Septiana ◽  
Suci Wulan Pawhestri ◽  
Nurhaida Widiani ◽  
Rina Budi
Keyword(s):  
Water Content ◽  
Water Concentration ◽  
Sweet Taste ◽  
Skin Extract ◽  
Black Rice ◽  
Crude Fiber Content ◽  
Washing Process ◽  
Fruit Skin ◽  
Dragon Fruit ◽  
Washing Water

Nata de leri is one of the products produced from fermented organic white, red, black rice washing water with the addition of red dragon fruit skin extract. In javanese the rice washing water is called Leri so that the resulting nata is called “Nata De Leri”. (carbohydrates, proteins, minerals) that are carried away from the rice membrane during the washing process. So that it can be utilized by microorganisms as a medium in making nata. The addition of red dragon fruit skin extract gives color because it contains a lot of anthocyanin and also the fiber and water content is very high. The purpose of this research was to determine the organoleptic and chemical characteristics of nata. This research used a qualitative descriptive method using a 2 factor complete randomized design and the treatment given was 75% of white, red, black rice washing water concentration, The concentration of red dragon fruit skin extract 75%, 50%, and 25%. The results showed that the most preferred texture on the addition of 75% red dragon fruit skin extract was an average of 4,65, the most preferred color in the addition of 75% and 25% red dragon fruit skin extract, the aroma of the average panelist doesn’t like all the aroma of nata de leri, for the taste all the samples were liked by the panelists because of their sweet taste. While the water content and crude fiber content produced are quite high on average but do not exceed SNI.

scholarly journals The Effect Ultrasound and Surfactants on Nanobubbles Efficacy against Listeria innocua and Escherichia coli O157:H7, in Cell Suspension and on Fresh Produce Surfaces

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2154
Author(s):  
Shamil Rafeeq ◽  
Reza Ovissipour
Keyword(s):  
Escherichia Coli ◽  
Cell Suspension ◽  
Sodium Dodecyl ◽  
Listeria Innocua ◽  
Wash Water ◽  
Tween 20 ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Bacterial Removal ◽  
The Impact

Removing foodborne pathogens from food surfaces and inactivating them in wash water are critical steps for reducing the number of foodborne illnesses. In this study we evaluated the impact of surfactants on enhancing nanobubbles’ efficacy on Escherichia coli O157:H7, and Listeria innocua removal from spinach leaves. We evaluated the synergistic impact of nanobubbles and ultrasound on these two pathogens inactivation in the cell suspension. The results indicated that nanobubbles or ultrasound alone could not significantly reduce bacteria in cell suspension after 15 min. However, a combination of nanobubbles and ultrasonication caused more than 6 log cfu/mL reduction after 15 min, and 7 log cfu/mL reduction after 10 min of L. innocua and E. coli, respectively. Nanobubbles also enhanced bacterial removal from spinach surface in combination with ultrasonication. Nanobubbles with ultrasound removed more than 2 and 4 log cfu/cm2 of L. innocua and E. coli, respectively, while ultrasound alone caused 0.5 and 1 log cfu/cm2 of L. innocua and E. coli reduction, respectively. No reduction was observed in the solutions with PBS and nanobubbles. Adding food-grade surfactants (0.1% Sodium dodecyl sulfate-SDS, and 0.1% Tween 20), did not significantly enhance nanobubbles efficacy on bacterial removal from spinach surface.

scholarly journals The Chlorine Reduction in Edamame by Water-Ozonated Minimally Process

2021 ◽  
Vol 1 (3) ◽  
Author(s):  
Silvia Oktavia Nur Yudiastuti ◽  
Rizza Wijaya
Keyword(s):  
Contact Time ◽  
Research Method ◽  
Residual Chlorine ◽  
Titration Method ◽  
Research Results ◽  
Washing Process ◽  
Ozonation Time ◽  
Water Based ◽  
Washing Water ◽  
Ozonated Water

The objective of this article was to study the effect of washing edamame using ozonated water to reduce residual chlorine contained in edamame through a continuous washing process. Chlorine residue in edamame was caused by the use of fertilizers and pesticides that contain chlorine compounds. The research method used was descriptive explanatory research consist of two treatment levels of ozonation time (0, 10, and 15 minutes) and ozone contact time (0, 12, 18, and 24 minutes) which were repeated twice. The residual chlorine and pesticide concentrations were calculated using the titration method. Based on research results, ozone can reduce chlorine in edamame. The lowest detected chlorine was in edamame which had been washed using ozone water with an ozonation time of 15 minutes with the amount of chlorine reduced by 56%. The longer the contact time, the greater the amount of reduced chlorine and the lower the concentration of ozone remaining in the washing water. Based on the research results, a brief conclusion that can be explained was ozone could reduce chlorine in edamame caused by fertilizer and disinfectant overuse until 50%.

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