Efficacy of An Aqueous Morinda citrifolia Fruit Extract-Phytosome Gel in Treating Oral Inflammatory Ulcer in Rabbit Model

Background: Oral inflammatory ulcers are one of the common complaints of patients attending out-patient clinics. Previous in vivo studies had shown that an Aqueous M. citrifolia Fruit Extract (AMFE) possessed anti-inflammatory and ulcer healing activities. Therefore, a standardized topical bioadhesive gel containing AMFE-phytosome was developed and determined for its oral ulcer healing efficacy in a rabbit model. Methods: The AMFE phytosome (AMFE-P) was prepared by a complexation method with the required amount of AMFE: Phosphatidylcholine: Tween 80 to weigh ratio of 2:1:0.2. Poloxamer 407 was used as a gelling agent. The oral ulcer was induced in male New Zealand white rabbits by topical application of acetic acid. Each test compound was applied to the ulcer for 10 days beginning on the second day after the ulcer induction. Complete ulcer healing on the specimen obtained on day 12 was observed histologically using the histological scoring protocol. Results: The optimized gel containing AMFE-P equivalent to AMFE 10%w/w (10%AMFE-P gel) showed the best bioadhesive gel quality, a smooth and homogeneous texture with an optimum viscosity and pH range used in human oral cavity, a good physical and chemical stability and the highest percentage cumulative release of total phenolic and scopoletin content. It was found that a daily application of 10% AMFE-P gel exerted a superior ulcer healing efficacy and a significantly rapid ulcer healing process than a twice daily application of topical gel containing AMFE 10%w/w or chlorhexidine 0.2%. Conclusion: These findings demonstrated that 10% AMFE-P gel has potential as a safe and effective alternative therapeutic agent for oral ulcers.

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Treatment of Oral Ulcers in Dogs Using Adipose Tissue-Derived Mesenchymal Stem Cells

Journal of Clinical Pediatric Dentistry ◽

10.17796/1053-4628-38.3.231 ◽

2014 ◽

Vol 38 (3) ◽

pp. 231-240

Author(s):

N M Alamoudi ◽

E A ElAshiry ◽

N M Farsi ◽

D A El Derwi ◽

H M Atta

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Growth Factor ◽

Ulcer Healing ◽

Cellular Therapy ◽

Healing Process ◽

Oral Ulcer ◽

Oral Ulcers ◽

Promising Tool

Aim:Adipose tissue Derived Mesenchymal Stem cells (ADMSCs) represent a promising tool for new clinical concepts in supporting cellular therapy. The goal of this study was to investigate the effects of ADMSCs transplantation on oral ulcer healing in dogs.Study design:Mesenchymal stem cells were isolated from adipose tissues of dogs obtained by suction-assisted lipectomy (liposuction), by dish adherence and were expanded in culture. Oral ulcers were induced by topical application of formocresol in the oral cavity of 18 dogs. The dogs were classified into 3 groups. Either autologousADMSCs, Corticosteriod (Dexamethasone) or vehicle (saline) was injected. The healing process of the ulcer was monitored histopathologically. Gene expression of vascular endothelial growth factor (VEGF),platelets derived growth factor (PDGF),epidermal growth factor (EGF) and collagen was assessed in biopsies obtained from all ulcers “as healing markers”, by real time polymerase chain reaction (PCR). Results:ADMSCs group showed significantly accelerated oral ulcer healing compared with the Dexamethasone and control groups. There was increased expression of VEGF, PDGF,EGF and collagen genes in ADMSCs-treated ulcers compared with Dexamethasone and controls. Conclusion:ADMSCs transplantation may help accelerate oral ulcer healing, possibly through the induction of angiogenesis by VEGF and PDGF, as well as epithelial and connective tissue proliferation as evidenced by increased EGF and collagen gene expression.

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Treatment of Oral Ulcers in Dogs Using Adipose Tissue-Derived Mesenchymal Stem Cells

Journal of Clinical Pediatric Dentistry ◽

10.17796/jcpd.38.3.193115427jg6vl60 ◽

2014 ◽

Vol 38 (3) ◽

pp. 215-222 ◽

Cited By ~ 4

Author(s):

N M Alamoudi ◽

E A El Ashiry ◽

N M Farsi ◽

D A El Derwi ◽

H M Atta

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Adipose Tissue ◽

Growth Factor ◽

Ulcer Healing ◽

Cellular Therapy ◽

Healing Process ◽

Oral Ulcer ◽

Oral Ulcers

Aim: Adipose tissue Derived Mesenchymal Stem cells (ADMSCs) represent a promising tool for new clinical concepts in supporting cellular therapy. The goal of this study was to investigate the effects of ADMSCs transplantation on oral ulcer healing in dogs. Study design: Mesenchymal stem cells were isolated from adipose tissues of dogs obtained by suction-assisted lipectomy (liposuction), by dish adherence and were expanded in culture. Oral ulcers were induced by topical application of formocresol in the oral cavity of 18 dogs. The dogs were classified into 3 groups. Either autologous ADMSCs, Corticosteriod (Dexamethasone) or vehicle (saline) was injected. The healing process of the ulcer was monitored histopathologically. Gene expression of vascular endothelial growth factor (VEGF), platelets derived growth factor (PDGF), epidermal growth factor (EGF) and collagen was assessed in biopsies obtained from all ulcers ‘’as healing markers’’, by real time polymerase chain reaction (PCR). Results: ADMSCs group showed significantly accelerated oral ulcer healing compared with the Dexamethasone and control groups. There was increased expression of VEGF, PDGF, EGF and collagen genes in ADMSCs-treated ulcers compared with Dexamethasone and controls. Conclusion: ADMSCs transplantation may help accelerate oral ulcer healing, possibly through the induction of angiogenesis by VEGF and PDGF, as well as epithelial and connective tissue proliferation as evidenced by increased EGF and collagen gene expression.

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Antibacterial and Therapeutic Potentials of the Capsicum annuum Extract against Infected Wound in a Rat Model with Its Mechanisms of Antibacterial Action

BioMed Research International ◽

10.1155/2021/4303902 ◽

2021 ◽

Vol 2021 ◽

pp. 1-17

Author(s):

Steve Endeguele Ekom ◽

Jean-De-Dieu Tamokou ◽

Victor Kuete

Keyword(s):

Antibacterial Activity ◽

Capsicum Annuum ◽

Rat Model ◽

Healing Process ◽

Antibacterial Properties ◽

Antibacterial Action ◽

Total Phenolic ◽

Infected Wound ◽

Fruit Extract ◽

Broth Microdilution Method

The wound healing process is essential to reform the damaged tissue and prevent its invasion by pathogens. The present study aims at evaluating the antibacterial and therapeutic properties of the Capsicum annuum L. (Solanaceae) extract against infected wound in a rat model with its mechanisms of antibacterial action. The fruit extract was prepared by maceration in methanol. The broth microdilution method was used to investigate the antibacterial activity of the methanol extract of C. annuum fruits. The therapeutic effect of the extract gel was performed on an excision wound infected with Staphylococcus aureus using a rat model. The total phenol, flavonoid, and tannin contents as well as the antibacterial mechanisms of action of the extract were determined using spectrophotometric methods. The C. annuum fruit extract showed antibacterial properties which can be linked to its total phenolic, flavonoid, and tannin contents. The antibacterial activity is due to the inhibition of the biofilm formation, ATPases/H+ proton pump, and dehydrogenase activity as well as the alteration of the bacterial cell membrane through the leakage of nucleic acids, reducing sugars and proteins. The extract gel showed a significant ( p < 0.05 ) increase in the percentage of wound closure and eradicated S. aureus at the infection site. The extract gel was nonirritating to the skin and slightly irritating to the eyes and should be used with caution. Overall, the findings of the present study support the traditional use of the studied plant in the treatment of wounds and infectious diseases associated with the tested bacteria.

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New insights of phenolic compounds from optimized fruit extract of Ficus auriculata

Scientific Reports ◽

10.1038/s41598-021-91913-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

M. Shahinuzzaman ◽

Parul Akhtar ◽

N. Amin ◽

Yunus Ahmed ◽

Farah Hannan Anuar ◽

...

Keyword(s):

Antioxidant Activity ◽

Bioactive Compounds ◽

Total Phenolic Content ◽

Phenolic Content ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Fruit Extract ◽

Optimum Extraction

AbstractIn this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH and ABTS as well as reducing power assays on total phenolic content (TPC). For the extraction purpose, the ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P < 0.0001) and total phenolic content (R2 = 0.961, P < 0.0001), indicating a significant correlation between the experimental and expected value. The highest DPPH radical scavenging activity was achieved 85.20 ± 0.96% at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC–ESI–MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

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Antioxidant Activities of the Aqueous Extracts of Pedalium murex D. Royen EX L. Fruit and Leafy Stem

European Journal of Medicinal Plants ◽

10.9734/ejmp/2021/v32i830406 ◽

2021 ◽

pp. 1-9

Author(s):

Gerard Bessan Dossou-Agoin ◽

Habib Ganfon ◽

Fidèle Assogba ◽

Adam Gbankoto ◽

Joachim Gbenou ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Lipid Peroxidation ◽

Antioxidant Activity ◽

Reducing Power ◽

Total Phenolic ◽

Scavenging Activity ◽

Aqueous Extracts ◽

Fruit Extract ◽

Stem Extract ◽

Dpph Scavenging

Aims: The leafy stem and fruit of P.murex have been reported to be used in folk medicine to treat male reproductive system ailments. This study was undertaken to assess the antioxidant potential of the aqueous extracts of P.murex leafy stem and fruit. Methodology: Extracts were prepared by macerating the powder in water. Total phenolics amount were determined by Folin-Ciocalteu reagent, flavonoids were quantified by aluminum chloride method and total tannin content was estimated by hexacyanoferric method. The in vitro antioxidant activity of the extracts were assessed through 2,2´-diphenyl-1-picrylhydrazyl (DPPH) scavenging assay, reducing power assay, hydrogen peroxide (H2O2) inhibition assay and lipid peroxidation assay. Results: Total phenolic compounds, flavonoids and tannins content were respectively equal to 48.91± 1.67 µg/mg Gallic Acid Equivalents (GAE); 56.01± 0.90 µg/mg Rutin Equivalents (RUE); 16.92± 1.22 µg/mg Tannic Acid Equivalents (TAE) for the leafy stem extract while they were equal to 26.26± 0.54 µg/mg GAE; 47.88± 2.39 µg/mg RUE; 7.94± 0.31 µg/mg TAE for the fruit. The leafy stem extract exhibited a more pronounced DPPH scavenging activity, reducing power, hydrogen peroxide scavenging activity and lipid peroxidation activity than the fruit extract. Conclusion: The antioxidant activity of the leafy stem aqueous extract was significantly more potent than that of the fruit extract. Further studies will find out the extracts pharmacological efficacy and innocuity.

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PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

ODONTO Dental Journal ◽

10.30659/odj.2.2.41-46 ◽

2015 ◽

Vol 2 (1) ◽

pp. 41

Author(s):

Umi Kulsum ◽

Ratnawati Hendari ◽

Siti Chumaeroh

Keyword(s):

Wound Healing ◽

Ulcer Healing ◽

Wistar Rats ◽

Povidone Iodine ◽

Healing Process ◽

Aloe Vera ◽

Control Group ◽

Significant Difference ◽

Male Wistar Rats ◽

Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

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Maclura pomifera (Osage Orange) Meyve Özütünden Elektroeğirme Yöntemiyle Üretilen Membranların Karakterizasyonu

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v6i4.447-451.1741 ◽

2018 ◽

Vol 6 (4) ◽

pp. 447

Author(s):

Rifat Battaloğlu ◽

Emine Müge Pekacar

Keyword(s):

Total Phenolic Content ◽

Phenolic Content ◽

Total Phenolic ◽

Fruit Extract ◽

Binding Properties ◽

Maclura Pomifera ◽

Electrospinning Method ◽

Phenolic Substances ◽

Ft Ir ◽

Resistant Structure

In this study, electrospinning method which is a common method of obtaining nanofibers is used. Nanolif was synthesized with chitosan-ethylenediamine tetraacetic acid / polyvinylalcohol (CS-EDTA / PVA) polymers using macula pomifera fruit extract. To obtain the nanofibers, 3% macula pomifera extract was added to the CS-EDTA / PVA solution. The structure and diameters of the resulting nanofibers were visualized using a scanning electron microscope (SEM). Chemical binding properties were determined by Fourier Transform Infrared Spectroscopy (FT-IR). Mass change due to nanofiber temperature was performed by Thermogravimetric analysis (TGA). At the end of the study, the total phenolic content of the extract and nanofiber was determined. The time dependent changes in the total phenolic content of nanolife have been investigated. The results obtained show that the nanofiber is loaded with the extract. It turns out that nanolipine is a temperature resistant structure. In addition, the phenolic compounds transfer to the nanofiber structure in a small amount of the extract. However, it has been determined that there is no significant change in the total amount of phenolic substances after nanofiber formation.

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EFEKTIVITAS ELEVASI EKSTREMITAS BAWAH TERHADAP PROSES PENYEMBUHAN ULKUS DIABETIK DI RUANG MELATI I RSUD DR. MOEWARDI TAHUN 2014

KOSALA : Jurnal Ilmu Kesehatan ◽

10.37831/jik.v3i1.47 ◽

2015 ◽

Vol 3 (1) ◽

Author(s):

Dwi Ariani Sulistyowati

Keyword(s):

Lower Extremity ◽

Ulcer Healing ◽

Healing Process ◽

Lower Extremities ◽

Tissue Perfusion ◽

Analysis Data ◽

Control Group ◽

Control Group Design ◽

Diabetic Ulcer ◽

Equivalent Control

AbstractIntroduction: People with Diabetes Mellitus have poor circulation, especially in areas far from heart, these causing the length of time of wounds healing. One of the interventions to improve the peripheral tissue perfusion of patients with Diabetic Ulcer is lower extremity elevation.Purpose: The purpose of this research is describe the characteristics of respondents, knowing Diabetic Ulcer healing process without lower extremity elevation, knowing Diabetic Ulcer healing process with lower extremity elevation and knowing the effectiveness of Diabetic Ulcer healing without lower extremity elevation and with lower extremity elevation.Research Methods: This research is to design an quasy experiment non equivalent control group design and analysis data used Independent T TestResearch Results: Results of this research is the elevation of lower extremities more effectively to increased Ulcers Diabetic healing process. It’s evidenced by Independent T Test obtained p = 0,000Conclusion: Elevation of lower extremities more effectively to increased Diabetic Ulcer healing in patients with Diabetic Ulcer in Melati I RSUD Dr. Moewardi.Advice: Lower extremity elevation expect can be applied in patients with Diabetic Ulcer.Key Words: Lower Extremity Elevation, Diabetic Ulcer Healing Process

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