scholarly journals Expression of estrogen receptors alpha (ERs α) and folliculogenesis profile in ovary of the rats ovarian hypofunction model

2021 ◽  
Vol 31 (1) ◽  
pp. 18-26
Author(s):  
Aulia Firmawati ◽  
Mitra Artha Kurnia Hutabarat ◽  
Herlina Pratiwi ◽  
Alibiruni Haryo
Keyword(s):  
Treatment Group ◽  
Ovarian Function ◽  
Follicular Development ◽  
Control Group ◽  
Reproductive Disorders ◽  
Test Analysis ◽  
Antral Follicles ◽  
Wistar Strain ◽  
Estrogen Binding ◽  
Hematoxylin Eosin

Ovarian hypofunction is one of the reproductive disorders that occur due to a decrease in ovarian function that causes animals to not experience heat. The purpose of this study was to determine the effect of GnRH antagonist hormone interventions on ovarian hypofunction model rats by looking at the expression of alpha ERs (ERs α) and the profile of folliculogenesis in the ovaries. This study used two groups of female Wistar strain rats, aged 8-10 weeks, bodyweight 150-180 grams and each group contained 10 animals. Intervention gave control group, without cetrorelix acetate intervention (placebo aqua dest sterile), treatment group with the induction of cetrorelix acetate 0.0135 mg/kg BW for 17 days. Observation of α ERs expression in the ovary was analyzed by immunohistochemical methods, and data were analyzed using T-Test analysis (α <0.05). Meanwhile, the folliculogenesis profile was analyzed by hematoxylin-eosin (HE) staining, then analyzed descriptively. The results of this study indicate that the treatment group given the intervention cetrorelix acetate dose 0.0135 mg/kg BW showed significantly different results compared to the control group. In the treatment group, the ERs α expression decreased by 82.7% compared to the control group and in the folliculogenesis profile, there was a decrease in follicular development from pre-antral follicles to antral follicles. Cetrorelix acetate interventions can have an effect on the inhibition of folliculogenesis and estrogen binding with ERs that cause anoestrous.

scholarly journals Ekstrak Etanol Daun Jati Belanda (Guazuma ulmifolia Lamk) Memperbaiki Profil Lipid Tikus (Rattus norvegicus) Wistar Jantan Dislipidemia

2020 ◽  
Vol 1 (1) ◽  
pp. 25-30
Author(s):  
Melita Hidajat ◽  
I Gusti Made Aman ◽  
Hendro Sukoco ◽  
Ferbian Milas Siswanto
Keyword(s):  
Treatment Group ◽  
Lipid Profile ◽  
Total Cholesterol ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Control Group ◽  
Wistar Strain ◽  
Before And After ◽  
Male Wistar Rats ◽  
Guazuma Ulmifolia

The purpose of this study was to prove that the administration of Jati (Guazuma ulmifolia Lamk) leaves extract improves the lipid profile of dyslipidemic male Wistar rats. Subjects were 20 rats (Rattus norvegicus), male, Wistar strain, dyslipidemia (total cholesterol ≥ 200 mg dl-1), aged 2 months old, weighing 180-200 grams. The control group (10 rats) were given a placebo of 3 ml aquadest (P0) and the treatment group was given extracts of the Jati (Guazuma ulmifolia Lamk) leaves extract of 25 mg kg-1 BW (P1). Before and after treatment for 14 days, total cholesterol, triglyceride, LDL, and HDL levels were examined. The results showed that in the P0 group there were no changes in total cholesterol, triglyceride, LDL, and HDL levels (p>0.05), whereas the P1 group experienced a decrease in total cholesterol, triglyceride and LDL levels (p<0.05) and an increase in HDL levels (p<0.05). The results of this study indicated that the Jati leaves extract was effective to improve the lipid profile of dyslipidemic rats. It was necessary to compare the effectiveness of Jati leaves extract with synthetic dyslipidemia drugs used in the community such as statin.

scholarly journals Corticosterone Injection Impairs Follicular Development, Ovulation and Steroidogenesis Capacity in Mice Ovary

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1047 ◽  
Author(s):  
Wei ◽  
Li ◽  
Meng ◽  
Zhang ◽  
Shen ◽  
...  
Keyword(s):  
Treatment Group ◽  
Horse Serum ◽  
Follicular Development ◽  
Control Group ◽  
Ovarian Weight ◽  
Stressful Environment ◽  
Methyl Sulfoxide ◽  
Ovarian Granulosa Cell ◽  
Serum Gonadotropin ◽  
Ovarian Index

The aim of this study is to establish an ovarian stress model, and to investigate the effects of stress on follicular development. Our data showed that continuous intraperitoneal injection of CORT successfully created a stressful environment in the ovary. To assess the effects of CORT on ovarian functions, 80 three-week-old ICR (Institute of Cancer Research) female mice were randomly divided into control group and treatment group. All mice were injected intraperitoneally with pregnant horse serum gonadotropin (PMSG). At the same time, the treatment group were injected with CORT (1 mg/mouse) at intervals of 8 h; while the control group was injected with same volume of methyl sulfoxide (DMSO). Blood, ovaries, or ovarian granulosa cell samples were collected at 24 h, 48 h, and 55 h after PMSG injection. The results showed that, compared with the control group, CORT-injected mice revealed a significant decrease in ovulation rates, ovarian weight, ovarian index, the number of secondary follicles and mature follicles, levels of estrogen and progesterone, and mRNA expression of steroid synthase-related genes. Collectively, our findings clearly demonstrated that CORT injection could represent an effective practice to simulate stresses that inhibit ovarian functions by reducing follicular development and ovulation.

Effect of exogenous gonadotrophins on ovarian morphology and oocyte maturation in the southern hairy nosed wombat Lasiohinus latifrons during the breeding season

2006 ◽  
Vol 18 (4) ◽  
pp. 477 ◽  
Author(s):  
C. H. McDonald ◽  
D. A. Taggart ◽  
W. G. Breed ◽  
G. V. Druery ◽  
G. A. Shimmin ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Follicular Development ◽  
Germinal Vesicle ◽  
Control Group ◽  
Antral Follicles ◽  
Nuclear Maturation ◽  
Significant Difference ◽  
Ovarian Morphology ◽  
The Mean ◽  
The Right

The effect of the exogenous administration of porcine follicle-stimulating hormone (pFSH) and pregnant mare serum gonadotrophin (PMSG) on ovarian follicular development and oocyte maturation in the southern hairy nosed wombat Lasiorhinus latifrons was investigated. Three experimental groups were administered pFSH at various doses and for different treatment lengths, followed by 25 mg porcine luteinising hormone (pLH) 12 h after the last dose of pFSH. Another group was given PMSG followed 72 h later by 25 mg pLH. Animals were killed 24 h after pLH. The left ovary was fixed for histology and the morphology of the antral follicles was determined, whereas follicular oocytes in the right ovary were aspirated, fixed, stained with 4′,6′-diamidino-2-phenylindole, and viewed for nuclear maturation. There was no significant difference in the mean number of ovarian follicles >1 mm, or in the size class of follicles assessed between control and experimental groups. However, a trend was observed suggesting a possible increase in follicles >3.0 mm in experimental groups compared with control animals. In all females administered exogenous porcine gonadotrophins, but not controls, some of the mural granulosa cells of large tertiary antral follicles had markedly enlarged nuclei (approximately 14 µm in diameter). All oocytes from the control group remained at the germinal vesicle stage, whereas approximately 40% of oocytes retrieved from the pFSH groups and 82.4% retrieved from the PMSG-primed animals had undergone germinal vesicle break down, with a small number reaching meiosis II. The present study shows that exogenous administration of either pFSH or PMSG to hairy nosed wombats can induce follicular growth and oocyte maturation. Such findings could be useful in the development of reproductive technology in this species.

scholarly journals Effectiveness of Boiled Figs (Ficus carica L), Rosy Periwinkle (Catharanthus roseus) and Cherry (Muntingia Calabura L.) on Decreasing SGOT and SGPT Serum in Male Wistar Strain Rats with Acute Hepatitis Model

2019 ◽  
Vol 7 (1) ◽  
pp. 744-755
Author(s):  
Grace Arni Yunita Hutajulu ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Acute Hepatitis ◽  
Catharanthus Roseus ◽  
Positive Control ◽  
Negative Control ◽  
Ficus Carica ◽  
Positive Control Group ◽  
Control Group ◽  
Wistar Strain ◽  
Muntingia Calabura

Acute hepatitis is inflammation of the liver. This disorder is usually caused by a virus or exposure to alcohol, drugs, toxic, or other pathogens. The purpose of this study was to determine the effectiveness of boiled figs leaves (Ficus carica L.), Rosy Periwinkle (Catharanthus roseus), and cherry (Muntingia Calabura L.) on decreasing serum levels of SGOT and SGPT. The objects in this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams. Rats were randomly divided into 3 groups: the treatment group, positive control and negative control. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. Boiled water of 0.3 grams Figs (Ficus carica L.), 3.5 grams of rosy Periwinkle (Catharanthus roseus), and 3.5 grams of cherry (Muntingia Calabura L.) was given as much as 3.6 cc orally for 7 days to treatment group. Data were analyzed with SPSS version 24, One Way ANOVA test was performed to compare SGOT and SGPT levels. The results showed a significant difference in SGOT levels between the treatment group, positive control group, and negative control group (p <0.05) and there were significant differences in SGPT levels between the treatment group and positive control group (p <0.05). The conclusions of this study were mixed  boiled of Figs (Ficus carica L.), rosy Periwinkle (Catharanthus roseus), and and Cherry (Muntingia Calabura L.) had an effect in decreasing serum SGOT and SGPT in male rats with acute hepatitis model.

scholarly journals Exploring the Effects of Automated Pronunciation Evaluation on L2 Students in Thailand

2021 ◽  
Vol 9 (3) ◽  
pp. 41-57
Author(s):  
Simon Moxon
Keyword(s):  
Speech Recognition ◽  
Treatment Group ◽  
Undergraduate Students ◽  
Mother Tongue ◽  
Control Sample ◽  
Control Group ◽  
Test Analysis ◽  
Automated Evaluation ◽  
Post Test ◽  
Predominantly Female

A significant barrier to effective communication in a second language is the awareness and accurate reproduction of phonetic sounds absent in the mother tongue. This study investigated whether the automated evaluation of phonetic accuracy using speech recognition technology could improve the pronunciation skills of 105 (88 female, 17 male) Thai undergraduate students studying English in Thailand. A pre-test, post-test design was employed using treatment and control sample groups, reversed over two six-week periods. Treatment group students were given access to an online platform on which they could record and submit their speech for automated evaluation and feedback via SpeechAce, a speech recognition interface designed to evaluate pronunciation and fluency. Independent samples t-test analysis of the results showed statistically significant improvement in pronunciation accuracy of students in the treatment group when compared to those in the control group (t (89) = 2.086, p = .040, 95% CI [.083, 3.423]), (t (89) = -4.692, p < .001, 95% CI [-5.157, -2.089]). Pearson’s correlation analysis indicated a weak to moderate, but statistically significant correlation between frequency of practise and pronunciation test score (r =.508, p < .001), (r = .384, p = .021). The study has limitations as the sample group was predominantly female, and time constraints limited students’ use of the software. Future studies should investigate possible gender differences and experiment with different forms of visual feedback.

scholarly journals Effectiveness of Boiled Comfrey Leaves (Symphytum Officinale L) on SGOT SGPT Serum in Male Wistar Strain Rats With Acute Hepatitis Models

2019 ◽  
Vol 7 (1) ◽  
pp. 756-767
Author(s):  
Rois Sahidin ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Acute Hepatitis ◽  
Serum Levels ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Symphytum Officinale ◽  
Wistar Strain

Acute hepatitis is an acute disease caused by viruses, alcohol and drugs, which is characterized by an increase in SGOT SGPT serum. The purpose of this study was to determine the effectiveness of boiled comfrey leaves (Symphytum Officinale L) levels of SGOT and SGPT serum in male wistar strain rats with acute hepatitis models. The objects in this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams. The objects were randomly divided into 3 groups: the treatment group, positive control and negative control. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. Boiled water of 2.8 grams of comfrey (symphytum officinale L) leaves was given as much as 2.7 cc orally for 7 days to the treatment group. Data were analyzed with SPSS version 24, one way ANOVA test was performed to compare serum levels of SGOT & SGPT. The results showed there were significant differences in serum levels of SGOT between the treatment group, positive control and negative control (p <0.05) and there were significant differences in SGPT results between the treatment group and positive control group (p <0.05) but there were no significant differences between the SGPT levels of the treatment group and the negative control group (p = 0.173). As Conclusion, boiled Comfrey leaf has an effect in decreasing serum SGOT & SGPT in male wistar strain rats with acute hepatitis model.

scholarly journals The Influence of Giving Tempe Flour Toward the Amount and Morphology of White Male Rat (Rattus norvegicus) Erythrocytes Wistar Strain was Affected by Cigarette Smoke

2019 ◽  
Vol 15 (2) ◽  
pp. 71
Author(s):  
I Made Subhawa Harsa
Keyword(s):  
Free Radicals ◽  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
White Male ◽  
Male Rat ◽  
Special Treatment ◽  
Control Group ◽  
Wistar Strain ◽  
Group 2

Abstract: Cigarette smoke was one of free radicals source for the body. Free radical contain in cigarette smoke were hydroxide radicals (•OH). Hydroxide radicals would cause erythrocytes damage. Antioxidants were substances that can prevent free radicals. Now, natural antioxidants source has developed, one of that is tempe, because of it contained antioxidants substances such as carotene, Vitamin E, isoflavon, and factor II antioxidants. This resource was for knowing the influence of giving tempe flour toward the amount and morphology of white male rat (Rattus norvegicus) erythrocytes wistar strain was affected by cigarette smoke. A pure experiment which was used the post test only-control group design method. Sample of the resource was white male rat wistar strain aged of 3-4 month as much 30 rats by the weight around 180-220 gram. The samples divided into 3 groups that were control group (K1), treatment group 1 (K2), and treatment group 2 (K3), each group consists of 10 rats. Control group was not given a special treatment, it only got a standard feed, treatment group 1 got special treatment and it was affected by cigarette smoke, furthermore, treatment group 2 got standard feed, it was affected by cigarette smoke and tempe. Analysis of data used one way anova experiment. The test showed the significance P-value = 0,036 that was <α (0,05). The result showed that there was the influence of giving tempe flour toward the amount and morphology of white male rat (Rattus norvegicus) erythrocytes wistar strain was affected by cigarette smoke. Keywords: cigarette smoke, free radicals, erythrocytes, antioxidants, tempe

198 EXOGENOUS CONTROL OF FOLLICULAR WAVE EMERGENCE IN WOOD BISON (BISON BISON ATHABASCAE)

2010 ◽  
Vol 22 (1) ◽  
pp. 257 ◽  
Author(s):  
R. B. McCorkell ◽  
W. Paziuk ◽  
L. Smart ◽  
M. R. Woodbury ◽  
G. P. Adams
Keyword(s):  
Ovarian Function ◽  
Follicular Development ◽  
Reproductive Technologies ◽  
Bison Bison ◽  
Control Group ◽  
New Wave ◽  
Handling Stress ◽  
Follicular Wave ◽  
End Point ◽  
Wood Bison

Two methods for synchronizing ovarian follicular development in both wood bison (Bison bison athabascae) and plains bison (Bison bison bison) were tested as part of a project to conserve wood bison through the application of advanced reproductive technologies. A secondary objective was to test the effect of a long-acting neurolept tranquilizer, pipothiazine palmitate, on ovarian function in bison. Female wood bison (4 years old; n = 14) and plains bison (2-8 years old, n = 10), previously conditioned to daily examination in a chute, were divided randomly into 3 groups in which 1) ovarian follicles ≥5 mm were ablated by ultrasound-guided transvaginal follicle aspiration, 2) 5 mg of estradiol-17β in canola oil was given i.m. or 3) no treatment was given (control). The experiment was conducted in 3 replicates so that each animal rotated through each of the groups. Half of the bison, blocked by subspecies and treatment group, were given a single dose (150 mg) of pipothiazine palmitate i.m. The ovaries were examined daily by transrectal ultrasonography beginning 4 days before treatment and continuing until the dominant follicle of a new wave reached a diameter of 10 mm. No effect of treatment with pipothiazine palmitate was detected for any end point. In addition, no differences were detected between wood and plains bison for any end point. Consequently the pipothiazine palmitate and subspecies treatment groups were collapsed for further analyses. The interval and variation in the interval to new follicular wave emergence were compared by ANOVA. Wave emergence was detected on Day 1.0 ± 0.2 (mean ± SEM; Day 0 = day of treatment) in the follicle ablation group, and was earlier (P < 0.05) than in both the estradiol (Day 3.3 ± 0.3) and control (Day 4.0 ± 0.4) groups. The interval to follicle wave emergence was least variable in the follicle ablation group (P < 0.05) and tended to be less variable in the estradiol group (P = 0.09) than in the control group (residuals, 0.1 ± 0.04, 1.0 ± 0.2, and 1.6 ± 0.3 days, respectively). Ovulations occurred subsequent to estradiol administration in 10 of 23 (43%) bison. In conclusion, ovarian follicular wave emergence can be synchronized in bison during the anovulatory season. Follicular ablation consistently shortened and decreased the variability in the interval to new wave emergence. The synchronizing effect of estradiol was confounded by the induction of ovulation. Progesterone will be added in future studies to control the ovulatory effect of estradiol. Pipothiazine palmitate had no discernable effect on ovarian function and maybe useful in reducing the effects of handling stress on untrained animals. Supported by a grants from the Advancing Canadian Agriculture and Agri-Food Fund, the Agri-Food Innovation Fund, Parks Canada, the World Wildlife Fund, and the Northwest Territories.

scholarly journals UJI EFEK ANALGETIK EKSTRAK ETANOL RIMPANG JAHE PUTIH (Zingiber officinale Rosc.var. Amarum) PADA TIKUS PUTIH JANTAN GALUR WISTAR (Rattus norvegicus)

PHARMACON ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 927
Author(s):  
Beatriks Lahamendu ◽  
Widdhi Bodhi ◽  
Jainer P. Siampa
Keyword(s):  
Treatment Group ◽  
White Male ◽  
Zingiber Officinale ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Analgesic Effects ◽  
Wistar Strain ◽  
Ginger Rhizome ◽  
Ethanol Extracts

ABSTRACTWhite Ginger Rhizome (Zingiber officinale Rosc.var. Amarum) contains secondary metabolites such as flavonoids, there are also several compounds such as gingerol, shogaol, and zhigeron which provide anti-inflamatory, antioxidant, analgesic, anticarsinogenic and cardiotonic effects. This study aims to determine the analgesic effect of ethanol extracts of white ginger rhizome with different doses, namely the treatment group 1, dose 0.006 g, the Treatment  Group 2, dose 0.012 g and the Treatment Group 3, dose 0.024 g tested on white male wistar strain rats. This study uses a laboratory experimental research design using male white rats as experimental animals. The result of the study using LSD showed a difference between the group of negative control and the group of positive control, and also the treatment group with extracts with significance value (0.014, 0.008, 0.012 and 0.005 ) the value <0.05, which indicates that according to the hypothesis namely rejecting H0 and accepting H1, which means significantly different between the negative control group with  positive control group and the treatment group with extracts, but there is no difference between the positive control group and the treatment group with extract which means there is no difference because they both have analgesic effects. From the results of the study it can be concluded that the ethanol extracts of white ginger rhizome at a dose 0.006 g, 0.012 g, and 0.024 g has analgesic effects in male white wistar strain rats can be seen from the decreasing number of rat responses (licks and jumps).Keywords: Analgesic, anova, White Ginger Rhizome, White male wistar strain ABSTRAKRimpang Jahe putih (Zingiber officinale Rosc.var amarum ) mengandung metabolit sekunder seperti flavonoid juga terdapat beberapa senyawa seperti gingerol, shogaol, dan zingeron yang memberikan efek antiinflamasi, antioksidan, analgetik, antikarsinogenik dan kardiotonik. Penelitian ini bertujuan untuk mengetahui adanya efek analgetik dari ekstrak etanol rimpang jahe putih dengan dosis yang berbeda-beda yaitu Kelompok Perlakuan 1 dosis 0,006 g, kelompok Perlakuan 2 dosis 0,012 g dan Kelompok Perlakuan 3 dosis 0,024 g yang diuji pada tikus putih jantan galur wistar. Penelitian ini menggunakan rancangan penelitian eksperimental laboratorium dengan menggunakan tikus putih jantan sebagai hewan percobaan. Hasil penelitian menggunakan LSD menunjukkan adanya perbedaan antara kelompok kontrol negatif dengan kelompok kontrol positif, dan juga kelompok perlakuan dengan ekstrak dengan nilai signifikansi (0,014, 0,008, 0,012 dan 0,005) nilai tersebut <0,05 yang menandakan bahwa sesuai hipotesis yaitu menolak H0 dan menerima H1 yang berarti berbeda secara signifikan antara kelompok kontrol negatif dengan kontrol positif dan kelompok perlakuan dengan ekstrak,tapi tidak terdapat perbedaan antara kelompok kontrol positif dengan kelompok perlakuan dengan ekstrak yang berarti tidak memiliki perbedaan karena sama-sama memiliki efek analgetik. Dari hasil penelitian dapat disimpulkan bahwa ekstrak etanol rimpang jahe putih dengan dosis 0,006 g, 0,012 g dan 0,024 g memiliki efek analgetik pada tikus putih jantan galur wistar dapat dilihat dari menurunnya jumlah respon tikus (jilatan dan lompatan).Kata kunci : Analgetik, anova, rimpang Jahe putih, tikus Putih jantan galur wistar

scholarly journals EFFECT OF STRENUOUS PHYSICAL ACTIVITY ON THE SUM OF LEUKOCYTES IN WISTAR STRAIN MALE MICE

2020 ◽  
Vol 1 (1) ◽  
pp. 1-4
Author(s):  
Annisa Nurul Hikmah ◽  
Chodidjah Chodidjah ◽  
Nur Anna Chalimah Sadyah
Keyword(s):  
Physical Activity ◽  
Treatment Group ◽  
The Body ◽  
Control Group ◽  
Male Mice ◽  
Control Group Design ◽  
A Value ◽  
Wistar Strain ◽  
Strenuous Physical Activity ◽  
Biomedical Laboratory

This research aims to determine the effect of strenuous physical activity on the sum of leukocytes. The study used experimentally with the posttest only control group design approach. The study subjects of 10 male mice were randomly divided into two groups. The control group was not given heavy physical activity and the treatment group was given heavy physical activity. On the 27th day of blood retrieval for an examination of the sum of leukocytes in the Integrated Biomedical Laboratory, in July-August 2020. The mean on the sum of leukocytes increased in the treatment group compared to the control group. The Mann Whitney test showed that the sum of leukocytes in the control group was significantly different from the treatment group with a value of p = 0.009 (p <0.05). More research is needed on strenuous physical activity in IL-6 levels and calculates leukocytes as one of the study boosters. This research can be used as a reference in the control of the immune system of the body.

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