scholarly journals Identification of Excretory Secretory (ES) Liquid Antigen Protein Fasciola gigantica with Polyethilen Glycol (PEG) Separation

2017 ◽  
Vol 21 (2) ◽  
pp. 125
Author(s):  
Yendri Junaidi ◽  
Ima Malawati ◽  
Made Sriasih
Keyword(s):  
Early Detection ◽  
Early Diagnosis ◽  
Western Blotting ◽  
Vaccine Candidate ◽  
Detection Method ◽  
Fasciola Gigantica ◽  
Molecular Weights ◽  
Antigen Protein ◽  
Secretory Products ◽  
Current Detection

Fasciola gigantica diagnosis usually performed by detection of worm eggs presence in the feces,but this conventional method has many disadvantages. Early diagnosis (early detection) cannot be performed in conventional methods because the worms in the host's body began to lay eggs at the age of 8–12 weeks of patency. The current detection method that is based on antibody­antigen reactions using excreted/secreted (ES) liquid by adult F. gigantica, is believed to be used for the early detection of fasciolosis. This study aimed to characterize the antigenic components of F. gigantica extretory/secretory products that could be used as a vaccine candidate development for early fasciolosis diagnostics. ES products were separated by PEG4000 at various concentrations (8%, 16%, 24%), then precipitates (pellets) obtained were dialyzed and characterized using SDS­PAGE and Western blotting. Results from SDSPAGE showed that there were 18 proteins bands with 7–70 kDa molecular weights. Western blotting on pellets derived from PEG separation at various concentrations affirmed that the proteins of 50, 25 and 20 kDa were antigenic at 8% PEG concentration, the 25 kDa and 50 kDa were antigenic at 16% PEG concentrations and the 25 kDa was antigenic at 25% PEG concentration

scholarly journals Comparison of the sensitivity of Western blotting between PVDF and NC membranes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yufang Xiang ◽  
Yuanyuan Zheng ◽  
Shaobo Liu ◽  
Gang Liu ◽  
Zhi Li ◽  
...  
Keyword(s):  
Western Blotting ◽  
Polyvinylidene Fluoride ◽  
Staining Intensity ◽  
Detection Sensitivity ◽  
Key Factors ◽  
Post Translational Modifications ◽  
Factors Affecting ◽  
Molecular Weights ◽  
Direct Blue ◽  
The Relationship

AbstractWestern blotting (WB) is one of the most widely used techniques to identify proteins as well as post translational modifications of proteins. The selection of electroblotted membrane is one of the key factors affecting the detection sensitivity of the protein which is transferred from gel to membrane in WB. The most common used membranes are polyvinylidene fluoride (PVDF) and nitrocellulose (NC) membranes. Which membrane of these two is more suitable for WB has not been reported so far. Here, by incubating proteins which were transferred to PVDF or NC membranes with a series of antibodies and different types of lectins, we investigated the relationship between the binding ability of these two membranes to proteins or glycoproteins and the molecular weight of the target protein. The antibody re-probed ability of the two membranes was also explored. Moreover, we verified the above results by directly incubating proteins having different molecular weights onto PVDF or NC membranes. Bound proteins were stained with direct blue-71, and the staining intensity was quantitated by scanning and densitometry.

scholarly journals Early Detection Methods for Silicosis in Australia and Internationally: A Review of the Literature

2021 ◽  
Vol 18 (15) ◽  
pp. 8123
Author(s):  
Emma K. Austin ◽  
Carole James ◽  
John Tessier
Keyword(s):  
Early Detection ◽  
Literature Review ◽  
Detection Method ◽  
Detection Methods ◽  
X Rays ◽  
Review Of The Literature ◽  
Occupational Lung Disease ◽  
Work Related ◽  
Growing Body ◽  
Future Work

Pneumoconiosis, or occupational lung disease, is one of the world’s most prevalent work-related diseases. Silicosis, a type of pneumoconiosis, is caused by inhaling respirable crystalline silica (RCS) dust. Although silicosis can be fatal, it is completely preventable. Hundreds of thousands of workers globally are at risk of being exposed to RCS at the workplace from various activities in many industries. Currently, in Australia and internationally, there are a range of methods used for the respiratory surveillance of workers exposed to RCS. These methods include health and exposure questionnaires, spirometry, chest X-rays, and HRCT. However, these methods predominantly do not detect the disease until it has significantly progressed. For this reason, there is a growing body of research investigating early detection methods for silicosis, particularly biomarkers. This literature review summarises the research to date on early detection methods for silicosis and makes recommendations for future work in this area. Findings from this review conclude that there is a critical need for an early detection method for silicosis, however, further laboratory- and field-based research is required.

scholarly journals Developing of a Simple Screening Tool for Opportunistic COPD Case Finding

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
Y M Mohamed ◽  
S H Sharkawy ◽  
D I Darwish
Keyword(s):  
High Risk ◽  
Early Detection ◽  
Early Diagnosis ◽  
Outpatient Clinic ◽  
World Wide ◽  
Screening Tool ◽  
Case Finding ◽  
Screening Questionnaire ◽  
Risk Patients ◽  
Simple Screening

Abstract Background Under diagnosis of COPD is serious problem in many countries world-wide because there are no generally detection tools available to detect high-risk patients for spirometry, and patients will not go for COPD check-up until a serious issue happens like exacerbation. Objective The aim of the work is trying to assess a new screening tool for early diagnosis of COPD. Patients and Methods The present study was conducted upon 500 subjects during the period from march 2018 to august 2018 who admitted to our chest department or visit our outpatient clinic, employees and visitors to Ain Sham hospitals.All subjects >40yrs who smoker or ex-smoker(≥10pack-years) applied a six variants(age,sex,packed years smoked during life ,dyspnea,chronic phlegmand chronic cohgh)questionnaire modified from PUMA questionnaire Subjects with score ≥5 did spirometry Results 500 subjects shared in the study 497 of them were males ( 99.4% )and 3 were females (.6%). 152of them(30.4%) had score <5 and 348 of them (69.6%)had score ≥5 who did spirometry.152 subjects did not perform spirometry. By spirometry we diagnosed 81(23.3) case COPD (fev1-fvc <.7) out of 348 subjects under gone spirometry and 16.2% of total subjects(500) . Conclusion Modified puma score is a simple and easy screening questionnaire for early detection of COPD cases and spirometry should be done to confirm the diagnosis or rule out.COPD is prevalent in many healthy apparent persons.

Immune responses of Texel sheep to excretory/secretory products of adult Haemonchus contortus

Parasitology ◽  
1994 ◽  
Vol 108 (3) ◽  
pp. 351-357 ◽  
Author(s):  
H. D. F. H. Schallig ◽  
M. A. W. van Leeuwen ◽  
W. M. L. Hendrikx
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Haemonchus Contortus ◽  
Lymphocyte Proliferation ◽  
The Other ◽  
Proliferation Index ◽  
Serum Samples ◽  
Immunoblotting Analysis ◽  
Molecular Weights ◽  
Secretory Products

SUMMARYThe excretory/secretory (E/S) products of adult Haemonchus contortus comprise of at least 15 polypeptides with molecular weights ranging from 10 to > 100 kDa. These E/S products induce an immune response in infected Texel sheep, as demonstrated by specific IgGI levels and a significant lymphocyte proliferation index. Moreover, immunoblotting analysis revealed that sera of primary H. contortus-infected sheep specifically recognize a 24 kDa E/S product. In addition, sera of challenged sheep react strongly with a 15 kDa E/S product. The other E/S products of H. contortus showed immunoreactivity with serum samples of Haemonchus-infected sheep as well as with samples of sheep harbouring other trichostrongylid infections. These cross-reacting epitopes are the main cause of the lack of specificity of an E/S material- based ELISA. This ELISA can differentiate Haemonchus infections from Nematodirus battus infections, but not from Ostertagia circumcincta or Trichostrongylus colubriformis infections.

scholarly journals Experimental Ehrlichia canis infection changes acute-phase proteins

2012 ◽  
Vol 21 (3) ◽  
pp. 206-212 ◽  
Author(s):  
Thiago Demarchi Munhoz ◽  
Joice Lara Maia Faria ◽  
Giovanni Vargas-Hérnandez ◽  
José Jurandir Fagliari ◽  
Áureo Evangelista Santana ◽  
...  
Keyword(s):  
Early Detection ◽  
Early Diagnosis ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
Ehrlichia Canis ◽  
Laboratory Findings ◽  
Early Detection Of Disease ◽  
Acid Glycoprotein ◽  
The Third ◽  
Canine Ehrlichiosis

Early diagnosis of canine ehrlichiosis favors prompt institution of treatment and improves the prognosis for the animal, since this disease causes mortality among dogs. Studies have shown that determining the concentration of acute-phase proteins (APPs) may contribute towards early detection of disease and aid in predicting the prognosis. This study aimed to evaluate the APP profile in dogs experimentally infected with Ehrlichia canis, at the start of the infection and after treatment. It also investigated whether any correlation between APP levels and the clinical and laboratory alterations over the course of the disease would be possible. The results obtained showed abnormal levels of all the APPs on the third day after infection (D3), with the highest levels being reached on D18, with the exception of ceruloplasmin and acid glycoprotein, which presented their peaks on D6 and D12 respectively. We concluded that assessment of APP levels could contribute towards establishing an early diagnosis of canine ehrlichiosis, particularly regarding acid glycoprotein and ceruloplasmin, since these proteins were detected at increased levels even before the onset of clinical and laboratory findings of the disease.

Optimized transitory ectopic expression of promastigote surface antigen protein in Nicotiana benthamiana, a potential anti-leishmaniasis vaccine candidate

2018 ◽  
Vol 125 (1) ◽  
pp. 116-123 ◽  
Author(s):  
Séverine Lacombe ◽  
Martine Bangratz ◽  
Jean-Paul Brizard ◽  
Elodie Petitdidier ◽  
Julie Pagniez ◽  
...  
Keyword(s):  
Surface Antigen ◽  
Nicotiana Benthamiana ◽  
Vaccine Candidate ◽  
Ectopic Expression ◽  
Antigen Protein

Localization and Recognition of Different Vertebra Tissues Based on Eddy Current Detection Method

2013 ◽  
Vol 740 ◽  
pp. 128-133 ◽  
Author(s):  
Wei Wen Liu ◽  
Kai Jiang ◽  
Hui Zhao ◽  
Cheng Long Wang
Keyword(s):  
Electrical Conductivity ◽  
Internal Fixation ◽  
Eddy Current ◽  
Biological Tissue ◽  
Detection Method ◽  
Pedicle Screws ◽  
Human Beings ◽  
High Penetration ◽  
Trauma Injury ◽  
Current Detection

The spine, which is throughout the trunk, is of great importance to the skeleton structure of human beings. For spine is vulnerable, the rate of spine incident trauma injury is relatively high. In the contemporary technology, the internal fixation prosthetics with implanting pedicle screws is the most efficient way in clinical treatment. However spine internal fixation surgery has many disadvantages like complex in the operation, the high penetration rate and inability to be used in the field battle. As a result, according to the magnetic property difference of different vertebra tissues, an eddy current detection method which is used in localization and recognition of different vertebra tissues to realize the navigation of spine internal fixation surgery is proposed in this paper. A set of improved series-reverse-three coils eddy current sensor is used to test the NaCl solution which electrical conductivity matches that of biological tissue. And the result of several experiments indicates this method can detect the biological tissue with the electrical conductivity no less than 0.5 S/m, which lays a solid foundation to further study of recognition different vertebra tissues.

scholarly journals Neonatal gastric perforation: is prematurity the only cause?

2020 ◽  
Vol 7 (9) ◽  
pp. 1318
Author(s):  
Manas R. Dash ◽  
S. K. Barma ◽  
Pranay Panigrahi ◽  
P. Parida ◽  
P. K. Mohanty ◽  
...  
Keyword(s):  
Early Detection ◽  
Early Diagnosis ◽  
Gastric Perforation ◽  
Primary Repair ◽  
Surgical Care ◽  
Abdominal Distension ◽  
Anterior Wall ◽  
Care Hospital ◽  
Neonatal Gastric Perforation ◽  
Gastric Ischemia

Background: Neonatal Gastric Perforation (NGP) is a serious and life-threatening emergency and challenging in terms of diagnosis and management.  The precise aetiology remains obscure in most cases. Published literatures provide theories of gastric ischemia, aerophagia & trauma with or without definite inciting pathology.  Owing to its high mortality rate, it requires early detection & urgent intervention to bring out the newborn from this catastrophe.Methods: We report five cases of neonatal gastric perforation over period of 2013-2019 admitted to a tertiary paediatric surgical care hospital managed by early detection, prompt resuscitation along with exploration and primary repair of the defect in gastric wall.Results: The common feature in these five cases were preterm age, low birth weight, hypoxic event   and   marked abdominal distension causing respiratory distress.  Features of imminent or complete septic shock were present in all cases. Perforation was found on anterior wall along greater curvature of stomach in all babies. Four babies out of five survived except in one case of jejunoileal Artesia that developed gastric perforation in post operative period.Conclusions: Neonatal gastric perforation should be suspected in cases of rapidly deteriorating premature newborn with gross abdominal distension and pneumoperitoneum. In our opinion, etiology of NGP is multifactorial. Prematurity, low APGAR score with vigorous resuscitation could be a clue for early diagnosis. Good outcome in our series was due to early diagnosis and prompt pre and postoperative measures. Additionally, sterile gastric content in peritoneum  might lead to survival from this catastrophic event.

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