scholarly journals Efek Antimikroba Pasta Gigi Kandungan Ekstrak Daun Teh 2% (Camellia sinensis) terhadap A. Actinomycetemcomitans

2016 ◽  
Vol 19 (1) ◽  
pp. 9 ◽  
Author(s):  
Juni Handajani
Keyword(s):  
Camellia Sinensis ◽  
Antimicrobial Effect ◽  
Positive Control ◽  
Diffusion Method ◽  
Tea Leaves ◽  
Zone Of Inhibition ◽  
Antimicrobial Effects ◽  
Effect Concentration ◽  
Tea Extract ◽  
Control Diffusion

Latar Belakang: Kandungan polifenol (catechin) dalam daun teh diketahui memiliki efek antimikroba terhadap beberapa bakteri. Derajat fermentasi terhadap daun teh akan mempengaruhi daya antimikrobanya. Pasta gigi dengan kandungan ekstrak daun teh segar 2% (Camellia sinensis) diharapkan memiliki efek antimikroba. Tujuan: pengetahuan ini bertujuan untuk mengetahui daya antimikroba pasta gigi kandungan ekstrak teh segar 2% terhadap bekteri A. actinomycetemcomitans. Metode Penelitian: bakteri A. actinomycetemcomitans diperololeh dari laboratorium MikrobiologiFakultas Kedokteran Hewan Universitas Gadjah Mada Yogyakarta. Pengenceran pasta gigi dengan kandungan teh 2% diencerkan menjadi 0,875%; 1,75%; 2,5%; 5%; 10%; dan 100% (kontrol positif). Metode difusi dilakukan pada 15 petri dengan cara masing-masing konsentrasi diambil 100 ul lalu diteteskan dalam setiap sumuran pada media BHI agar yang telah mengandung A. Actinomycetemcomitans kemudian petri dieramkan dalam inkubator selama 24 jam pada suhu 370 C. Pengukuran diameter zone hambatan disekitar sumuran menggunakan jangka sorong, dibantu dngan mikroskop, selanjutnya data dianalisis menggunakan AVANA dan LSD. Hasil menunjukkan daya anti bakteri pasta gigi kandungan ekstrak teh 2% terhadap bakteri A. Actinomycetemcomitans dimulai pada konsentrasi 5% dan terdapat perbedaan yang bermakna (p<0,01) pengaruh konsentrasi terhadap zone hambatan yang dihasilkan. Disimpulkan peningkatan konsentrasi pasta gigikandungan ekstrak teh akan meningkatkan daya antimikroba terhadap bakteri A. Actinomycetemcomitans.  Background: Polyphenols (catechins) in tea leaves are known to have antimicrobial effects against some bacteria. Degree of fermentation tea leaves will affect the antimicrobial. Toothpaste containing 2% tea leaves ectract (Camellia sinensis) is expected to have antimicrobial effects. Aim: this study was aimed to determine the antimicrobial effect of tootthpastes contain 2% tea extract on the bacteria A. Actinomycetemcomitans. Method: Aggregatibacter actinomycetemcomitans bacteria from Microbiology Laboratory, Faculty of Veterinary Medicine, Gadjah Mada University Yogyakarta. Tea extract-containing dentrifice was dilluted into 0,875%; 1,75%; 2,5%; 5%; 10%; dan 100% (as positive control). Diffusion method was used on 15 disks. Every concentration of tea extract-containing dentrifice was placed in bottomless cylinder in BHI agar disks that have been heavily seeded with A. Actinomycetemcomitans , then incubated for 24 hours at 370. The diameter of the clear zone of inhibition was measured using sliding caliper and microscope then data were analyzed using AVANA and LSD. The result showed that the antimicrobial effect of  tea extract-containing dentrifice was started at 5% and there were significantly difference (p<0,01) effect concentration of  tea extract-containing dentrifice on zone of inhibition. In conclusion, increasing concentration of  tea extract-containing dentrifice could increase the antimicrobial effect against A. Actinomycetemcomitans. 

scholarly journals Antimicrobial Effects of Three Different Treatment Modalities on Dental Implant Surfaces

2017 ◽  
Vol 43 (6) ◽  
pp. 429-436 ◽  
Author(s):  
Olav I. Larsen ◽  
Morten Enersen ◽  
Anne Karin Kristoffersen ◽  
Ann Wennerberg ◽  
Dagmar F. Bunæs ◽  
...  
Keyword(s):  
Dental Implants ◽  
Dental Implant ◽  
Er:Yag Laser ◽  
Antimicrobial Effect ◽  
Positive Control ◽  
Treatment Modalities ◽  
Bacterial Strains ◽  
Implant Surface ◽  
Antimicrobial Effects ◽  
Dental Implant Surface

Resolution of peri-implant inflammation and re-osseointegration of peri-implantitis affected dental implants seem to be dependent on bacterial decontamination. The aims of the study were to evaluate the antimicrobial effects of 3 different instrumentations on a micro-textured dental implant surface contaminated with an avirulent or a virulent Porphyromonas gingivalis strain and to determine alterations to the implant surface following instrumentation. Forty-five dental implants (Straumann SLA) were allocated to 3 treatment groups: Er:YAG laser, chitosan brush, and titanium curette (10 implants each) and a positive (10 implants) and a negative (5 implants) control. Each treatment group and the positive control were split into subgroups of 5 implants subsequently contaminated with either the avirulent or virulent P. gingivalis strain. The antimicrobial effect of instrumentation was evaluated using checkerboard DNA–DNA hybridization. Implant surface alterations were determined using a light interferometer. Instrumentation significantly reduced the number of attached P. gingivalis (P &lt; .001) with no significant differences among groups (P = .310). A significant overall higher median score was found for virulent compared with avirulent P. gingivalis strains (P = .007); the Er:YAG laser uniquely effective removing both bacterial strains. The titanium curette significantly altered the implant surface micro-texture. Neither the Er:YAG laser nor the chitosan brush significantly altered the implant surface. The 3 instrumentations appear to have a similar potential to remove P. gingivalis. The titanium curette significantly altered the microstructure of the implant surface.

scholarly journals AN INVITRO COMPARATIVE EVALUATION OF ANTICANDIDAL HERBS (GINGER & TURMERIC) ON STREPTOCOCCUS MUTANS

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Bafna Harshal P ◽  
Lunawat Sadhana R ◽  
Vathar Jagdishchandra ◽  
Kalantharakath Thanveer ◽  
Ricky Pal Singh ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Ethanolic Extract ◽  
Antimicrobial Effect ◽  
Positive Control ◽  
Negative Control ◽  
Dimethyl Formamide ◽  
Zone Of Inhibition ◽  
Ginger Extract ◽  
Inert Solvent

Aim: To comparatively evaluate the antimicrobial effect of turmeric and ginger extracts on Streptococcus mutans in in-vitro conditions. Material & Method: An in-vitro experimental study was conducted in a laboratory setting. Ethanolic extract of Ginger and Turmeric was prepared separately by cold masseration technique. The extract of each was then diluted with an inert solvent, Dimethyl Formamide, to obtain 5 different concentrations (2%, 4%, 6%, 8%, and 10%) of each. 0.2% chlorhexidine was used as a positive control and dimethyl formamide was used as negative control. The different extracts, along with controls, were then subjected to microbiological investigation to determine which gave a wider zone of inhibition against streptococcus mutans. The zone of inhibition was measured in millimeters. Results: Turmeric extracts presented the largest zone of inhibition 33mm at the concentration of 8%, while Ginger extract showed a zone of inhibition of 34mm at the concentration of 10%. Conclusion: Ethanolic extract of Turmeric demonstrated antimicrobial activity against Strptococcus Mutans at a lower concentration than that of Ginger. Keywords: Ginger, Turmeric, Streptococcus Mutans.

scholarly journals Possible Tracheal Relaxant and Antimicrobial Effects of the Essential Oil of Ethiopian Thyme Species (Thymus serrulatus Hochst. ex Benth.): A Multiple Mechanistic Approach

2021 ◽  
Vol 12 ◽  
Author(s):  
Najeeb Ur Rehman ◽  
Mohd Nazam Ansari ◽  
Tesfay Haile ◽  
Aman Karim ◽  
Khalil Y Abujheisha ◽  
...  
Keyword(s):  
Essential Oil ◽  
Guinea Pig ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Response Curves ◽  
Relaxant Effect ◽  
Antimicrobial Effects ◽  
Low Concentrations ◽  
Pde Inhibition ◽  
The Right

The genus Thymus is traditionally used for the treatment of hyperactive airways complaints. The purpose of the current study is to investigate the potential tracheal relaxant effect and possible mechanism(s) of the essential oil of Thymus serrulatus (TS Oil) in isolated guinea pig tracheal tissues. The essential oil was obtained from the fresh erial parts of Thymus serrulatus, and its phyto-components were identified by GC-MS analysis. Guinea pig tracheal preparations were used for testing the tracheal relaxant effect of TS Oil with the determination of the mechanism(s) involved in this relaxation. GC-MS findings reveal that terpenes, fragrance constituents, saponins, and higher fatty acids are present in TS Oil. In isolated guinea pig trachea, TS Oil inhibited carbachol (CCh, 1 µM) and K+ (80 mM)-induced contractions in a pattern similar to that of dicyclomine. TS Oil, at 0.3 mg/ml, shifted parallel CCh-curves towards the right, followed by a non-parallel shift at higher concentration (1 mg/ml), thus suppressing maximum response in the same manner as produced by dicyclomine. Pretreatment of tissues with TS Oil (1 and 3 mg/ml) also produced a rightward shift of Ca++ concentration-response curves (CRCs) in the same manner as caused by verapamil. Further, TS Oil at low concentrations (0.3 and 1 mg/ml) shifted isoprenaline-induced inhibitory CRCs towards the left and increased cAMP levels in isolated tracheal homogenates similar to papaverine, a phosphodiesterase (PDE) inhibitor. In the antimicrobial assay performed by the agar well diffusion method, TS Oil was found most active against Candida albicans and Staphylococcus aureus where the zone of inhibition measured was 28 mm. Additionally, there was little difference between standard strains of gram-positive and gram-negative bacteria. However, methicillin-resistant S. aureus (MRSA) showed a small zone of inhibition as compared to standard strains (22 mm). From these results, it can be concluded that the essential oil of T. serrulatus has the potential to produce antimicrobial effects while causing tracheal relaxation mediated possibly by anticholinergic effects, Ca++ channel blockade, and PDE inhibition whereas additional mechanism(s) cannot be ruled out.

scholarly journals Antibacterial activity of the dregs of green tea leaves (Camellia sinensis L.) on Staphylococcus epidermidis as causes of acne

2017 ◽  
Vol 1 (2) ◽  
pp. 1-5
Author(s):  
Naniek Widyaningrum ◽  
Badie'ah Badie'ah ◽  
Sri Lestari
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Staphylococcus Epidermidis ◽  
Economic Value ◽  
Diffusion Method ◽  
Tea Leaves ◽  
Green Tea Leaves

The dregs of green tea leaves have not been used until today, it is still considered as a waste that is not useful. It is known that the dregs of the green tea leaves contain Epigallocatechin-3-gallate (EGCG), which is quite high. EGCG has benefits as an antibacterial. The long-term goal of this research is the application of cosmetic preparation products from the dregs of green tea leaves that have effectiveness, stability and high economic value. The specific target of this research is to get the dose and concentration of the dregs of green tea leaves in a stable and effective way as anti-acne against Staphylococcus epidermidis (S. epidermidis). The search of the dregs of green tea leaves is by techniques of infundation of liquid fractions with ethyl acetate, identification of EGCG compound by using HPLC, antibiotics test against S. epidermidis was conducted by diffusion method. The results showed that the content of EGCG of the dregs of green tea leaves was 21.325% w/w, and the concentration of 1% of the dregs of green tea leaves can inhibit the growth of S. epidermidis well.

scholarly journals ANTIBACTERIAL AND ANTIFUNGAL ACTIVITY OF HEDYOTIS CORYMBOSA, INULA RACEMOSE, HOLOSTEMMA ADAKODIEN MEDICINAL PLANTS

2019 ◽  
pp. 25-29
Author(s):  
AJIT KUMAR MARISETTI ◽  
VENKATARATHANAMMA V.
Keyword(s):  
Antifungal Activity ◽  
Medicinal Plants ◽  
Microbial Activity ◽  
Positive Control ◽  
Diffusion Method ◽  
Antibacterial And Antifungal Activity ◽  
Zone Of Inhibition ◽  
Activity Screening ◽  
Antibacterial And Antifungal ◽  
Anti Fungal

Objective: To evaluate the antibacterial and antifungal activity of three different medicinal plants invitro. Oil extracts of the plants from three different families (Hedyotis corymbosa, Inula racemose, Holostemma adakodien) are used to find the antibacterial and antifungal activity. Methods: Study of anti-microbial activity using agar cup diffusion method, inoculums preparation, Anti-bacterial activity screening (well diffusion method), Anti-fungal activity screening (well diffusion method) are the different methods which are used to find the anti-bacterial and anti-fungal activities of the oil extracts of the above medicinal plants. The activity of these oil extracts and the selected antibiotics are evaluated against four bacterial strains (E. Coli, S. aureus and P. aeruginosa) and three Candida fungal strains (albicans, parapsilosis and tropicalis.). The anti microbial activity of Hedyotis corymbosa, Inula racemose, Holostemma adakodien oil extracts zone of inhibition evaluation was compared with positive control of fungal strain Fluconozole and bacterial strain Norfloxacin, Cefepime and Gatifloxacin. Results: The anti microbial activity of Hedyotis corymbosa, Inula racemose, Holostemma adakodien oil extracts zone of inhibition evaluation was compared with positive control of fungal strain Fluconozole and bacterial strain Norfloxacin, Cefepime and Gatifloxacin. Further studies are needed to explore the novel antibacterial bioactive molecules. Conclusion: The plants Hedyotis corymbosa, Inula racemose and Holostemma adakodien have shown significant anti-bacterial and anti-fungal activity.

scholarly journals FORMULATION OF LOTION FROM BLACK TEA EXTRACT (CAMELLIA SINENSIS LINNAEUS) AS SUNSCREEN

2019 ◽  
Vol 11 (1) ◽  
pp. 205 ◽  
Author(s):  
Iyan Sopyan ◽  
Riestya Dwi Permata ◽  
Dolih Gozali ◽  
Insan Sunan Kurniawan Syah
Keyword(s):  
Camellia Sinensis ◽  
Black Tea ◽  
Ultraviolet Rays ◽  
Tea Leaves ◽  
Security Testing ◽  
Black Tea Extract ◽  
Emulsifying Agent ◽  
Irritation Test ◽  
Tea Extract ◽  
Tea Leaf

Objective: The goal of the project was to explore extracts from black tea leaves (Camellia sinensis Linnaeus) to prove it can give protection against ultraviolet rays (UV) in lotion preparation.Methods: Black tea extract was made in sunscreen lotion in oil in water (O/W) type emulsion using a combination of PEG-8 and beeswax as emulsifying agent 1, and combination of cetyl alcohol, ceteth-20, and steareth-20 as emulsifying agent 2. Evaluation of lotion including phytochemical screening of black tea leaf extract, measurement of sun protecting factor (SPF) value of extract black tea leaves, measurement of SPF preparation lotion sunscreen extract black tea leaves, physical observation of preparation, qualitative preparation evaluation using thin layer chromatography (TLC) and lotion security testing have been done to ensure the quality of lotions.Results: The result exhibited the effective SPF that was different to the sunscreen lotion F1, which contained 0.03% w/v and F2 which contain of 0.04% w/v black tea leaves extract with the point of SPF is 20.31 and 24.71 respectively. Both formulas fulfilled the requirements as lotion preparations and did not irritate the skin based on an irritation test on 20 volunteers.Conclusion: Formulas F1 and F2 can be applied as a sunscreen with good physical quality and is safe for topical use in lotion preparation.

scholarly journals Antimicrobial Effects of Several Essential Oil from Aromatic Plants

2016 ◽  
Vol 8 (4) ◽  
pp. 477-481 ◽  
Author(s):  
Felicia TUŢULESCU ◽  
Maria DINU ◽  
Alexandru Radu CORBU ◽  
Elena Iuliana IONIȚĂ
Keyword(s):  
Bacillus Subtilis ◽  
Essential Oil ◽  
Alternaria Alternata ◽  
Antimicrobial Effect ◽  
Penicillium Expansum ◽  
Diffusion Method ◽  
Aromatic Plants ◽  
Antimicrobial Effects ◽  
Sweet Basil ◽  
Basil Oil

Essential oils (EOs) have been long recognized for their antibacterial, antifungal, antiviral, insecticidal and antioxidant properties. The present research aimed to study the antimicrobial effects of some volatile oils from aromatic plants (sweet basil and dill) against several microorganisms, namely Bacillus subtilis, Alternaria alternata and Penicillium expansum. The oils have been extracted through distillation procedures and the antimicrobial action of the oils was assessed through the disc diffusion method. The best effect against the Bacillus subtilis strain has occurred when the essential oil of dill was undiluted. Regarding the the Alternaria species, it was noted that dill volatile oil has acted in an efficient way only undiluted. As the oil's concentration decreased, the strain becomed resistant. The sweet basil oil has proven to be highly effective when acting against the Bacillus strain. By volatilization, the sweet basil oil produced a strong antimicrobial effect, even in control disc, in which it was noticed a small development of colonies comparing with the dill oil. The results indicated that the sweet basil essential oil exerted an antimicrobial effect both against the tested bacteria and moulds, while the dill oil had a great inhibitory action on Bacillus subtilis and Alternaria alternata, but was less efficient against Penicillium expansum. 

Antimicrobial Effect of Squid Ink on Common Microbial Causing Biofilm Attaching to Silicone

2015 ◽  
Vol 804 ◽  
pp. 191-194
Author(s):  
Duongruitai Nicomrat ◽  
Jirasak Tharajak
Keyword(s):  
Heat Treatment ◽  
Room Temperature ◽  
Microbial Growth ◽  
Antimicrobial Effect ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
Plastic Substrates ◽  
Food Applications

Present study consisted a possible novel natural antimicrobial agent replacing chemicals for preventing the microbial growth and microbial biofilm accumulated on silicone. Since squid ink has long proved to play various primary roles in wide ranges of alternative therapeutic medicine and food applications. This research was designed to report the potential antimicrobial effects of squid ink especially after heat treatment against the microorganisms causing biofilms. Various temperature conditions comprising room temperature (RT), 40, 60, 80, and 100°C were applied to the crude squid ink. Two fresh ink samples isolated from splendid squid (Loligo duvauceli) and soft cuttlefish (Sepioteuthis lessoniana) were determined for their antimicrobial activities after heat treatment on most reported microbes causing biofilms on these plastic substrates. The results showed that after pretreatment at RT-50°C for 15 min, fresh crude ink ofL.duvauceliconsistently presented higher bioactive activity (> 15 mm diameter) thanS.lessoniana(5-10 mm diameter), based on a zone of inhibition of disc diffusion method. From this study, both squid inks could definitely be future applied as active antimicrobial ingredients into silicone.

scholarly journals EVALUATION OF BIOLOGICAL ANTIMICROBIAL EFFECT OF ALOE VERA ON COLIFORM ISOLATES IN LEACHATE FROM A DUMPSITE

2021 ◽  
pp. e275
Author(s):  
Joshua Olu ◽  
Yusuf Hajara Oyiza ◽  
Charles Evbomai Ikhide ◽  
Henry Okokoro
Keyword(s):  
Aloe Vera ◽  
Antimicrobial Effect ◽  
Confirmatory Test ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Disc Diffusion Method ◽  
E Coli ◽  
High Level ◽  
And Storage ◽  
Storage Temperatures

This study is to evaluate the biological disinfectant ability of Aloe vera (under different conditions and parameters) on coliform isolates from leachate that emanate from a dumpsite.Leachate samplewas subjected to a presumptive test to evaluate the presence of coliforms in them; the positive samples were further subjected to a confirmatory test to identify the thermo-tolerant nature of the coliform. The coliforms were thermo-tolerant Escherichia coli (FTE) and Thermo-tolerant coliform (non E. coli) (FTC) Disinfectant efficacies of Aloe vera (with various condition and parameters) were evaluated on the isolated microorganisms from the leachate samples, using the disc diffusion method. The Aloe vera showed that concentration of the disinfectant matters in its efficacy with 5% storage concentration showing high level of zone of inhibition with the isolated microorganisms. Also the Aloe vera was more effective at a lower pH and storage temperatures of 0, 25 and 35oC.

scholarly journals Antimicrobial Effect and Transdentinal Diffusion of New Intracanal Formulations Containing Nitrofurantoin or Doxycycline

2014 ◽  
Vol 25 (5) ◽  
pp. 425-429 ◽  
Author(s):  
Ana Rita Silva ◽  
Elizabete Brasil Santos ◽  
Shelon Cristina Souza Pinto ◽  
João Carlos Gomes ◽  
Irene Pina Vaz ◽  
...  
Keyword(s):  
Antimicrobial Effect ◽  
Root Surface ◽  
Positive Control ◽  
Diffusion Method ◽  
Dentinal Tubules ◽  
Agar Diffusion ◽  
Agar Diffusion Test ◽  
Agar Surface ◽  
Microbial Growth Inhibition

The aim of this study was to investigate in vitro the antimicrobial effect and diffusion against E. faecalis of new intracanal medications on the external root surface. The medications tested were a placebo gel (PC); the new formulations with either 3% nitrofurantoin (NIT) or 3% doxycycline hydrochloride (DX) and 2% chlorhexidine (CHX) gel as positive control. The new formulations were tested using the traditional agar diffusion test (ADT) and an adapted agar diffusion method (AADM), where the teeth were filled with the medications and left to diffuse on agar surface seeded with E. faecalis. In the ADT, the larger zones of microbial growth inhibition were seen in DX, followed by CHX and NIT. In the AADM test only DX and CHX showed antimicrobial effect. Statistically significant differences between groups were observed by the Kruskal-Wallis test (2=47.126; p<0.001). The new intracanal formulations with DX and NIT have demonstrated antimicrobial effect against E. faecalis, but only DX was able to diffuse through the dentinal tubules and exert antimicrobial effect outside the roots.

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