scholarly journals The Effect of Pegagan (Centella Asiatica (L.) Urban) Ethanol Extracts on Hippocampal PSD-95 Protein Expression in Male Wistar Rats

2018 ◽  
Vol 23 (3) ◽  
pp. 85
Author(s):  
Adibah Ferhad ◽  
Auliyani Andam Suri ◽  
Astri Handayani ◽  
Sri Redjeki ◽  
Ria Kodariah
Keyword(s):  
Protein Expression ◽  
Wistar Rats ◽  
Memory Function ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Wistar Rat ◽  
Immunohistochemical Method ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats

One effort to overcome the decline in memory function is through herbal medicine. Pegagan (Centella asiatica (L.) Urban) contain the active components of triterpenoid and flavonoids, has been known to be able to improve memory function. Synaptic plasticity is the basis of memory formation which is strongly influenced by synapse proteins such as PSD-95. Loss of PSD-95 protein can cause memory function decline. This study aims to determine the effect of 70% ethanol extract of pegagan toward PSD-95 protein expression on hippocampus of male Wistar rat. Eighteen male Wistar rats were randomly divided into 3 groups, 6 rats/group: group (1) given ethanol extract of pegagan with dose 300 mg / kgBW / day (CA300), (2) given ethanol extract of pegagan with dose 600 mg / kgBW / day (CA600), and (3) control group (K), given daily aquadest. All three groups were treated for 28 consecutive days. At the end of the treatment period, rats were decapitated and the hippocampus was isolated from the brain. Analysis of protein was done by immunohistochemical method. Statistical analysis was performed by One Way ANOVA parametric test followed by Post-Hoc Bonferroni. The results showed that there was no significant difference between group K and group CA300 (P = 0.123), whereas there were significant differences between CA600 group and K group and CA300 group (P = 0.000). From this research, it can be concluded that 70% ethanol extract of pegagan with dose 600mg / kgBW / day can increase expression of PSD-95 protein on hippocampus of male Wistar rat.

scholarly journals The effect of ethanol extract of pegagan (Centella asiatica) on the performance of Wistar rats after restraint stress

2010 ◽  
Vol 8 (1) ◽  
pp. 11-16
Author(s):  
BAARID LUQMAN HAMIDI ◽  
SAMIGUN SAMIGUN ◽  
ANIK LESTARI
Keyword(s):  
Restraint Stress ◽  
Wistar Rats ◽  
Radial Maze ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
The Body ◽  
Control Group ◽  
Error Type ◽  
Stress Group ◽  
Significant Difference

Hamidi BL, Samigun, Lestari A. 2010. The effect of extract ethanol of pegagan (Centella asiatica) on the performance of Wistar rats after restraint stress. Biofarmasi 8: 11-16. The aim of this research was to investigate the effects of extract ethanol of pegagan (Centella asiatica) after treated with restraint stress by measuring the eight arms radial maze performance of rats. Pre-test and post-test controlled groups design was applied in this research. Male Wistar rats (Rattus novergicus) with the mean age of 8 weeks and the body weight of 150-200 grams which used for sample were divided randomly into 4 groups, each group consisted of 6 rats, i.e. (i) control group (without any treatment), (ii) stress group (it was given by restraint stress for 2 hours/day for each rat), (iii) pegagan group (it was given by 0.3 mg/g BW/day/rat extract ethanol of pegagan), and (iv) pegagan and stress group (it was given by 0.3 mg/g BW/day/rat extract ethanol of pegagan and restraint stress for 2 hours/day for each rat). The treatments were given for 21 days. Within 12 days for each pre-treatment and post-treatment, a test on the eight arm radial maze was conducted on individual rat to observe its performance. The assessment of rat performance in the eight arms radial maze test was conducted based on error type B. Kruskal-Wallis and Mann-Whitney tests with SPSS for Windows 16 version were applied to analyze statistically the difference between four groups. Kruskal-Wallis test was used to show the significant performance level difference between four groups of rats with p=0.001, while Mann-Whitney test was used to determine the significant difference between stress group and pegagan group (p=0.001), also stress group and pegagan and stress group (p=0.001). The result of research showed that there was no significant difference between control group and stress group (p=0.051), control group and pegagan group (p=0.143), control group and pegagan and stress group (p=0.143), also pegagan group and pegagan and stress group (p=0.952). It was concluded that extract ethanol of pegagan improved the performances of rats on the eight arms radial maze after treated with restraint stress.

scholarly journals Centella asiatica ethanol extract increases hippocampal brain derived neurotrophic factor in male Wistar rats

2018 ◽  
Vol 37 (2) ◽  
pp. 143
Author(s):  
Astri Handayani ◽  
Sophie Yolanda ◽  
Ria Kodariah
Keyword(s):  
Synaptic Plasticity ◽  
Protein Expression ◽  
Adult Male ◽  
Wistar Rats ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Memory Formation ◽  
Male Rats ◽  
Hippocampal Region ◽  
Male Wistar Rats

Background<br />Synaptic plasticity, which primarily takes place in the hippocampus, is the molecular basis of long- term memory formation. Brain derived neurotrophic factor (BDNF), a member of the neurotrophin family, plays a significant role in synaptic plasticity and memory formation. When BDNF is released, it binds to its receptor and activates various intracellular signal transduction pathways leading to synaptic plasticity. Several methods to improve memory function in humans have been studied, one of which is the use of herbal compounds, such as Centella asiatica (CeA), an herbaceous plant that has been used for improving memory. This study aims to examine the effects of CeA ethanol extract on BDNF protein expression in the CA1 hippocampal region in adult male rats.<br /><br />Methods<br />A randomized experimental design was performed involving 18 adult male Wistar rats. The rats were randomized into three groups: one control/distilled water group and two groups treated with doses of CeA ethanol extract of 300 mg/kgBW (CeA300) and 600 mg/kgBW (CeA600), respectively. CeA ethanol extract was administered orally for 28 consecutive days with weekly weight-adjusted dose. After 28 days, the rats were decapitated, and the hippocampus was isolated from the brain. BDNF protein expression was assessed using immunohistochemistry. Data was analyzed using Kruskal-Wallis test and continued with post-hoc analysis. <br /><br />Results<br />There was a significant increase in BDNF protein expression in the CeA600 group compared to the control group (p&lt;0.001). <br /><br />Conclusion<br />Administration of CeA ethanol extract increased BDNF protein expression in the CA1 hippocampal region of adult male rats.

scholarly journals Cardioprotective effects of Citrullus lanatus rinds in male wistar rats

2020 ◽  
Vol 44 (1) ◽  
pp. 123-129
Author(s):  
O. G. Akintunde ◽  
E. S. Ajibola ◽  
S. A. V. Abakpa ◽  
B. O. Oluwo ◽  
J. O. Olukunle
Keyword(s):  
Risk Ratio ◽  
Wistar Rats ◽  
Citrullus Lanatus ◽  
Ethanol Extract ◽  
Cardiac Risk ◽  
Control Group ◽  
Dependent Manner ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Dose Dependent

Water melon, citrullus lanatus is a common edible fruit belongs to the family of cucumber (Cucurbitacea). The outer part of citrullus lanatus known as the rind is always discarded. This study investigated the effects of ethanol extract of citrullus lanatus rinds orally administered on some liver function enzymes, kidney function markers, cardiac risk ratio, and the atherogenic coefficient indices in male wistar rats. Twenty-five male Wistar albino rats were randomly distributed into five groups (I, II, III, IV and V) of five rats each received 0mg/kg, 100 mg/kg, 200mg/kg, 400mg/kg and 800mg/kg of ethanol extract of Citrullus lanatus rinds respectively for 35days. The result showed that the effects of ethanol extract of citrullus lanatus rinds was not significant difference (p > 0.05) in serum liver enzymes (Aspartate aminotransferase, Alanine aminotransferase, Alkaline phosphatase) in increasing dose dependent manner when compared with control group. There were no significant differences (p > 0.05) in serum levels of urea and creatinine with increasing dose of ethanol extract of Citrullus lanatus rinds in rats when compared with control group. The extract showed significantly higher (p< 0.05) values in the serum HDL, cholesterol but there were significant reduction (p< 0.05) in the serum total cholesterol, triglycerides, LDL, VLDL and non-HDL values in rats at higher doses of the extract when compared with control group. While it was observed that the cardiac risk ratio, the atherogenic coefficient and indices decreased significantly (p< 0.05) in rats at increasing doses of ethanol extract of Citrullus lanatus rinds when compared with control group. This may suggest a likely cardio-protective effects of ethanol extract of Citrullus lanatus rinds in increasing dose dependent manner in rats when compared with control group. In conclusion, it can be inferred that Citrullus lanatus rinds can be consumed by animals without detrimental effect.

scholarly journals The Analysis of Osteoblast Cell Number on Femur Fractures Provided Red Spinach Extract (Amaranthus tricolor L.)

2019 ◽  
Vol 5 (1) ◽  
pp. 45
Author(s):  
Ferdian Nugroho ◽  
Aris Prasetyo ◽  
Muhammad Hasan
Keyword(s):  
Oxidative Stress ◽  
Fracture Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Ethanol Extract ◽  
Cell Number ◽  
Control Group ◽  
Osteoblast Cell ◽  
Significant Difference ◽  
Male Wistar Rats

Bone fracture is a musculoskeletal injury with a high incidence rate. The healing process of the fracture can be inhibited by oxidative stress, which occurs due to Reactive Oxygen Species (ROS) that exceeds the antioxidant capacity in the body that neutralizes it. Antioxidants that have red spinach have the potential to suppress the level of oxidative stress. This study aims to determine the effect of red spinach ethanol extract on the healing process of fracture in male Wistar rats through osteoblast cell count. A sample of 30 male Wistar rats was divided into five groups; negative control group, positive control group, and three groups of red spinach ethanol treatment with doses of 35.4 mg / 150 g body weight (BW), 70.8 mg / 150 gBW, and 141.6 mg / 150gBW induced fracture, splinted, then treated for one week. One Way Anova test results showed a significance of 0.000 (p <0.05) in which there was a significant difference in the osteoblast level between the treatment group and the control group. The result of LSD test between P1 group and P3 group showed significantly different result where the increase of osteoblast cell number was in line with the increase of the dose of red spinach ethanol extract. The conclusion is that red spinach ethanol extract has a positive impact during the fracture healing process with osteoblast cell number parameters. Keywords: Red spinach ethanolic extract, fracture healing process, osteoblast, oxidative stress

scholarly journals Bitter Leaves Ethanol Extract (Vernonia amygdalina) Decreases Total Cholesterol Serum Level of Hypercholesterolemic Male Wistar Rats

2018 ◽  
Vol 2 (2) ◽  
Author(s):  
Ivana C Hasan ◽  
Hendra Subroto ◽  
Grace Puspasari
Keyword(s):  
Total Cholesterol ◽  
Wistar Rats ◽  
Plasma Lipid ◽  
Ethanol Extract ◽  
Serum Levels ◽  
Control Group ◽  
Standard Diet ◽  
Group Vi ◽  
Significant Difference ◽  
Male Wistar Rats

Hypercholesterolemia is an important risk factors of atherosclerosis. Bitter leaf is believed to have potency in decreasing plasma lipid. The aim of this study is to study the effect of bitter leaf ethanol extract in decreasing total cholesterol serum levels on hypercholesterolemic male Wistar rats. This is a true experimental laboratory study with complete randomized design. Antidyslipidemia effect was tested with HFF induction and Propiltiourasil 0,01% towards 30 rats which were divided into 6 groups: groups I as normal control (standard diet), groups II, III, and IV were given bitter leaf ethanol extract of 100 mg/kgBW, 200 mg/kgBW, and 400 mg/kgBW dose, group V as positive control group with Simvastatin, and group VI as negative with aquades. HFF  were given for 14 days then the study was continued for 14 days. The percentage data of the declining total cholesterol levels before and after treatments is analyzed with ANAVA, continued with LSD test. The result between group II, III, and IV with group VI shows a significant difference p<0,01. We concluded bitter leaf ethanol extract can decrease the level of total cholesterol serum of hypercholesterolmic Wistar rats.

Pengaruh Profilaksis Ekstrak Ubi Jalar Ungu (Ipomoea batatas L.) terhadap Kadar Malondialdehida Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Wistar yang Diinduksi Karagenan

2018 ◽  
Vol 15 (2) ◽  
pp. 146
Author(s):  
BRILIAN DINANTI ◽  
FITRI HANDAJANI
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Wistar Rats ◽  
Group Method ◽  
Control Group ◽  
Intraplantar Injection ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Potato Extract ◽  
Purple Sweet Potato

<p>Liver is an organ with complex metabolism. When the liver is inflamed, cellular immunity will defend against inflammatory agents by stimulating immune cells to produce reactive oxygen species (ROS). Excessive ROS accumulation cause oxydative stress with increased  liver malondialdehyde (MDA) level. Some researches showed that purple sweet potato contain flavonoids (anthocyanins) that functioned as antioxydants. This study aimed to show the prophylactic effect of purple sweet potato extract to the liver MDA level of male Wistar rats induced by carrageenan.</p><p>This study used post-only control group method using 18 male Wistar rats divided into 3 groups: group of rats without treatment, group of rats induced by 0,1 ml of 1% carrageenan by intraplantar injection on day-8, and group of rats given with 872 mg/kgBW of purple sweet potato extract for 7 days and induced by 0,1 ml of 1% carrageenan. In the end of the study, the liver MDA levels were measured by Thio-Barbituric Acid method on each groups.</p><p>The results of One-Way ANOVA test showed there was no significant difference (p = 0,290) between group of rats without treatment (<em>x̅</em>= 207,50) and group of rats induced by carrageenan (<em>x̅</em>=233,17). Then, there is no significant difference (p = 0.978) between group of rats induced by carrageenan and group of rats given with prophylactic purple sweet potato extract and induced by carrageenan (<em>x̅</em>= 232,50).</p><p>The conclusion of this study is giving intraplantar injection of carrageenan can increase liver MDA level insignificantly and giving prophylactic purple sweet potato extract has an effect to decrease the liver MDA level of rats induced by carragenan insignificantly because it contains anthocyanins as antioxidants.</p><p> </p><strong>Keywords: </strong>Liver, <em>Ipomoea batatas</em> L., Malondialdehyde, Anthocyanins

Potential Antiseptic of Rhodomyrtus tomentosa leaves extract on Healing wound in Male Wistar rats

2021 ◽  
pp. 3143-3149
Author(s):  
Endang Sri Purwanti Ningsih ◽  
Noorlaila Noorlaila ◽  
Ikhwan Rizki Muhammad ◽  
Windy Yuliana Budianto
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Wound Care ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Rhodomyrtus Tomentosa

Background: The process of wound healing is influenced by various factors such as age, hormones, and wound care. Wound care is done to accelerate wound healing which can be done by various methods, one of them is traditional care. Traditional wound care can use medicinal plants. Rhodomyrtus tomentosa is a medicinal plant that has an antioxidant, anti-inflammatory, antitumor and antibacterial content. Thus this study aims to evaluate the effectiveness of the antiseptic solution of the Rodhomyrtus tomentosa leaf extract on wound healing in male Wistar rats. Method: this research is pure experimental research with post test only control group design. Thirty male white rats were divided into five groups, namely negative control, positive control, Rhodomyrtus tomentosa leaf extract 15%, 30%, and 60%. Rhodomyrtus tomentosa leaf extraction was carried out by maceration method with 70% ethano solvent. The extraction results are divided into 3 concentrations (15%, 30% and 60%). The wound healing process was evaluated by measuring the length of the wound manually from 0 to 10 days in each group. Meanwhile, the number of fibroblast cells was calculated through hematoxylin eosin (HE) staining and observed using an Olympus CX41 microscope with a 10x magnification and objective lens magnification in 3 fields. Result: There was a significant difference in the reduction in wound length (p =< 0,000) between the five experimental groups (Rhodomyrtus tomentosa leaf extract solution 15%, 30% and 60%, negative control and positive control. Solution of rhodomyrtus tomentosa leaf extract accelerated the increase in the number of fibroblasts compared to the negative control group (p = 0.003), but did not make a difference (p = 0.403) with the positive control group. Rhodomyrtus tomentosa leaf extraction solution had the same microscopic effect on the number of fibroblasts with a positive control group given 0.9% NaCl solution. Conclusion: There was a significant difference in the number of fibroblasts between all groups, but no difference in wound healing length.

scholarly journals PENGARUH PEMBERIAN GEL KOMBINASI EKSTRAK GETAH PEPAYA (Carica papaya L) DAN EKSTRAK DAGING LIDAH BUAYA (Aloe vera) TERHADAP PROSES PENYEMBUHAN ULKUS TRAUMATIKUS PADA MALE WISTAR RATS YANG MENDERITA DIABETES MELITUS

2015 ◽  
Vol 2 (1) ◽  
pp. 41
Author(s):  
Umi Kulsum ◽  
Ratnawati Hendari ◽  
Siti Chumaeroh
Keyword(s):  
Wound Healing ◽  
Ulcer Healing ◽  
Wistar Rats ◽  
Povidone Iodine ◽  
Healing Process ◽  
Aloe Vera ◽  
Control Group ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Papaya Latex

Introduction: Research on the use of papaya and aloevera sap on wound healing each been done, but effects the combination of the two extracts on wound healing in diabetes mellitus condition has not been done.Purpose: This study aims to determine the effect of a gel combination of papaya latex and aloevera flesh extracts on the traumatics ulcer healing process in diabetic rats.Methods: True experimental laboratory research with the post-test-only control group design that performed on 28 male wistar rats which were divided intofour groups: DM and non-DM rat that treatment with povidone iodine and gel combination of papaya latex and aloevera flesh extract. The treatment was done for 7 days with smearing that was given 2x/day. Traumatics ulcer healing seen from the number of fibroblasts that then were analyzed by One WayAnova and post hoc LSD test.Results: The results showed the number of fibroblasts in DM and non-DM rat with povidone iodine were 44.6 and 42.73; in the DM and non-DM rat with a gel combination of papaya latex and aloe vera flesh extracts were 61.10 and 77.03. One Way Anova test resulted p-value = 0.000, it was mean that there were at least a significant difference on the number of fibroblasts in the two groups. Differences of the number fibroblasts was shown in all groups, except in the group DM and non-DM rats with povidone iodine (p = 0.764).Conclusion: Conclusion of the study was the provision of a gel combination of papaya latex and aloevera flesh extracts effect on the traumatics ulcer healing process in diabetic rats.

scholarly journals PENGARUH EKSTRAK DAGING LIDAH BUAYA (Aloe Vera) TERHADAP PENYEMBUHAN ULSERASI MUKOSA MULUT PADA MALE WISTAR RATS

2015 ◽  
Vol 1 (1) ◽  
pp. 25
Author(s):  
Laila Fitrotuz Zahroh ◽  
Rahmawati Sri Praptiningsih ◽  
Moh. Baehaqi
Keyword(s):  
Oral Mucosa ◽  
Wistar Rats ◽  
Healing Process ◽  
Research Result ◽  
Aloe Vera ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Male Wistar Rats

Background: Oral mucosa ulceration which often occurs usually in the form of white-yellowish spot with concave surface, reddish edge and pain. Based on previous research, Aloe vera process anti-inflammation substance that could help quickening ulceration healing process. This research aims to know the effect of Aloe vera flesh extract on Male wistar rats oral mucosa ulceration in-vivo. Method: this research was quasi experimental research with the post-test only control group design using Male wistar rats as the testing animal. In the research, there were three treatment groups: The first groups which was given aquadest treatment, second groups with Aloe vera flesh extract, and third groups which was given chlorhexidine gluconate 0,2% treatment. The data collecting was based on histopathology observation concerning the increase of fibroblast quantity. Result: The research result based on comparison test among the three groups with One Way Anova showed that on Day 3th, the average quantity of fibroblast didn't have significant difference between the treatment group and control group positive that was p>0,05, meanwhile on Day 7th every group showed significant difference p<0,05. Conclusion: It concluded that Aloe vera flesh extract has influence on the healing of Male wistar rats oral mucosa ulceration as shown by fibroblast increasing quantity.

scholarly journals The Hepatoprotective Effect of Ethanol Extract of Syzygium campanulatum (Korth) and Syzygium aromaticum (L) Leaf on Male Wistar Rat (Rattus Norvegicus) was Induced by Paracetamol

2017 ◽  
Vol 15 (2) ◽  
pp. 196
Author(s):  
Much Ilham Novalisa Aji Wibowo ◽  
Nur Aeni ◽  
Zidna Mazayatul Huda ◽  
Nunuk Aries Nurulita
Keyword(s):  
Wistar Rats ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hepatoprotective Activity ◽  
Group Iv ◽  
Control Group ◽  
Syzygium Aromaticum ◽  
Male Wistar Rats ◽  
Hepatoprotective Agent ◽  
Better Than

Syzygium campanulatum and Syzygium aromaticum contains antioxidant components suchas flavonoids, phenolic, and terpenoids. May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotec Syzygium campanulatum and Syzygium aromaticum contains antioxidant components such as flavonoids, phenolic, and terpenoids.May have hepatoprotective properties in reducing SGPT and SGOT activity. This research wants to determine the potency of hepatoprotective of ethanolic extract of Syzygium campanulatum (Korth) and Syzygium aromaticum leaf compared with curcuma tablets. This research uses 24 male Wistar rats divided into 6 groups: I, II, III (as a normal, induction, and compared control), group IV, V, VI were treated 105, 210, and 420 mg/kg BW respectively. The study was conducted for 9 days. After 7 days of treatment, treated groups were exposed by hepatotoxic dose of paracetamol (2000 mg/kg BW). The SGPT and SGOT activity of all groups was measured by enzimatic assay. The result can be concluded that Syzygium campanulatum extract was found to be active as hepatoprotective agent with 210 mg/kg BW dosage (SGPT 21.76 ± 3.98 U/L and SGOT 7.32±6.74U/L) as eff ective as with the curcuma tablets (SGPT 23.91 ± 4.41 U/L and SGOT 14.12±5.37 U/L) and the hepatoprotective activity of Syzygium campanulatum extract at a dosage 420 mg/kg BW better than curcuma tablets (SGPT 12.43 ± 6.51 U/L and SGOT 6.64 ± 5.88 U/L). While the hepatoprotective activity of Syzygium aromaticum extracts eff ective as with curcuma tablets at all dosage variation.

Sign in / Sign up

Export Citation Format

Share Document