scholarly journals PROSES PENGELOLAHAN MENINGKATKAN AKTIVITAS HIPOLIPIDEMIK JAMUR TIRAM (PLEUROTUS OSTREATUS) PADA TIKUS (RATTUS NORVEGICUS) SPRGUE-DAWLEY HIPERKOLESTEROL

2012 ◽  
Vol 2 (1) ◽  
Author(s):  
Eni Harmayani, Deera Army Pramana, Sri Anggrahini dan Sutikarini
Keyword(s):  
Rattus Norvegicus ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Male Rats ◽  
Oyster Mushroom ◽  
Sprague Dawley ◽  
Low Density Lipoprotein Cholesterol ◽  
High Lipid ◽  
High Lipid Diet ◽  
Lipid Diet

Oyster mushroom is considered to have hypocholesterolemic and hypolipidemic activities. Therefore, it is classifiedas functional food. Prior to serving and consumption, oyster mushroom can be processed in various ways. Thisresearch studied the effect of three kinds of processing commonly used in cooking oyster mushroom; boiling,frying, and roasting. Thirty Sprague-Dawley male rats, 8 weeks old, were acclimated to laboratory condition,and then induced with high lipid diet. The rats were divided into five experiment groups; high-lipid diet (K),high-lipid diet + raw oyster mushroom (M), high-lipid diet + fried oyster mushroom (G), high-lipid diet + roastedoyster mushroom (P), and high-lipid diet + boiled oyster mushroom (R). Blood samples were obtained from orbitalplexus after acclimation, hypercholesterolemic induction, and 21 days of feeding. The blood serum was examinedfor total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c), dan high-densitylipoprotein cholesterol (HDL-c). The result showed that cooked oyster mushroom had better hypocholesterolemicand hypolipidemic activity than raw oyster mushroom. Among the three, the roasted oyster mushroom reduced thetotal cholesterol the most, while boiled oyster mushroom reduced triglyceride the most.

scholarly journals Modulatory Effects of Some Fruit Juices on Lipid Profile in Rats Fed with High Lipid Diet

2020 ◽  
pp. 1-8
Author(s):  
Habibu Tijjani ◽  
Enoch Banbilbwa Joel ◽  
Carrol Domkat Luka
Keyword(s):  
Lipid Profile ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Fruit Juices ◽  
Atherogenic Index ◽  
Ananas Comosus ◽  
Low Density ◽  
High Lipid ◽  
High Lipid Diet ◽  
Lipid Diet

Background: Hyperlipidemia is a predisposing factor to several cardiovascular diseases, including atherosclerosis, myocardial infarction, heart attacks and others. Aim: This study investigate the hypolipidemic properties of five selected fruits using rats pre-fed with High Lipid Diet (HLD) under experimental conditions. Methods: Raw fruit juices from Guava (Psidium guajava), Pawpaw (Carica papaya), Banana (Musa acuminata), Apple (Pyrus malus) and Pineapple (Ananas comosus) respectively, were administered orally at a dose of 7 ml/kg body weight for 14 days. Sera from the experimental rats were prepared for total cholesterol (TC), triglyceride (TG), High Density Lipoprotein (HDL) and Low Density Lipoprotein (LDL) assay, while Very Low Density Lipoprotein (VLDL) and Atherogenic Index (AI) were computed. Results: Feeding the experimental rats with High Lipid Diet increased their levels of TC, TG, LDL, VLDL and AI by 68.73%, 72.94%, 20.23%, 71.79% and 208.72% respectively with a corresponding decrease in HDL by 29.47%. Pineapple juice significantly reduced the levels of TC, TG, LDL, VLDL and AI by 84.44%, 69.39%, 95.19%, 70.15% and 96.99% respectively. Guava, banana and apple treated groups produced 56.72%, 23.88% and 07.46% increase in HDL respectively, with a non-significant (p<0.05) value in pawpaw treated group, when compared to the HLD untreated control. Phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, cardiac glycosides, terpenes and steroids in all fruit juices and the absence of balsams and phenols. Conclusion: Consumption of the selected fruit juices improved lipid profile and reduced the risk associated with hyperlipidemia in experimental rats.

scholarly journals Uricase-deficient rat is generated with CRISPR/Cas9 technique

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8971 ◽  
Author(s):  
Yun Yu ◽  
Nan Zhang ◽  
Xianxiang Dong ◽  
Nan Fan ◽  
Lei Wang ◽  
...  
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Urate Oxidase ◽  
Male Rats ◽  
Wild Type ◽  
Sprague Dawley ◽  
Normal Ranges ◽  
Model Animal ◽  
Renal Changes ◽  
One Year

Urate oxidase (uricase, Uox) is a big obstacle for scientists to establish stable animal models for studying hyperuricemia and associated disorders. Due to the low survival rate of uricase-deficient mice, we generated a Uox-knockout model animal from Sprague Dawley (SD) rats using the CRISPR/Cas9 technique by deleting exons 2 to 4 of the Uox gene. The uricase-deficient rats were named “Kunming-DY rats”, and were apparently healthy with more than a 95% survival up to one year. The male rats’ serum uric acid (SUA) increased to 48.3  ± 19.1 µg/ml, significantly higher than those of wild-type rats. Some indexes of the blood fat like total triglyceride, low density lipoprotein, and renal function indexes including blood urea nitrogen and serum creatinine were significantly different from those of wild-type rats, however, all the indexes were close to or in normal ranges. Histological renal changes including mild glomerular/tubular lesions were observed in these uricase-deficient rats. Thus, “Kunming-DY rats” with stable uricase-deficiency were successfully established and are an alternative model animal to study hyperuricemia and associated diseases mimicking human conditions.

scholarly journals Effect of Hyper and Hypothyroidism on Lipid Profile and Liver Function of Male Rats

2011 ◽  
Vol 8 (4) ◽  
pp. 926-933
Author(s):  
Baghdad Science Journal
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Physiological Saline ◽  
Serum Levels ◽  
Lipoprotein Cholesterol ◽  
Male Rats ◽  
Control Group ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Significant Difference

This study was designed to investigate the effect of thyroid hormone disturbance on lipids profiles and liver functions. Eighteen mature male rats Rattus norvegicus were divided into three groups. The first and the second groups were injected subcutaneously with thyroxine (T4) and carbimazol (both at 600 µg/kg BW) respectively on alternate days, to produce recurrent periods of hyper and hypothyroidism .The control group which is the third group was injected with physiological saline. The process continued 4 weeks, after that, injection, blood specimens were collected to estimate serum levels of T3 and T4, Total cholesterol (TC), Triglycerides (TG), High density lipoprotein cholesterol (HDL-C), Low density lipoprotein cholesterol (LDL-C) and Very low density lipoprotein cholesterol (VLDL-C) were determined in the serum. In addition to that, we measure GOT, GPT and CPK enzymes activity. The results showed no significant difference in body weight and a significant increase (P

scholarly journals Blood Lipids and Its Atherogenic Indices in Alloxan Induced Diabetic Male Rats

2020 ◽  
pp. 25-33
Author(s):  
O. D. Chikezie ◽  
S. C. Meludu ◽  
I. S. I. Ogbu ◽  
B. N. Egejuru ◽  
T. Ude ◽  
...  
Keyword(s):  
Blood Lipids ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Lipoprotein Cholesterol ◽  
Male Rats ◽  
Low Density Lipoprotein Cholesterol ◽  
Atherogenic Indices ◽  
Diabetes Induction

Background: Diabetes mellitus is a group of metabolic disorders which result to excessive accumulation of blood sugar over a prolonged period. Due to higher risk of diabetes mellitus to cardiovascular disease, it is crucial to identify and address these cardiovascular risks. This study assessed the effects of diabetes on levels of some blood lipids and its atherogenic indices in diabetic male rats. Methods: This is an experimental study that involved 40 apparently healthy adult male albino rats (wistar strain) which were randomly assigned to five groups (A, B, C, D and E) of eight (8) animals each. Group A (Normal Control of No intervention for 72 hours), Group B (Diabetic rats of 72 hours post diabetes induction), Group C (metformin treated diabetic rats), Group D (Diabetic Control untreated) and Group E (Normal Control of 3 weeks post diabetes induction). Seven milliliters of fasting blood sample were collected from all the subjects. Serum levels of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-c), low density lipoprotein cholesterol (LDL-c) and very low density lipoprotein cholesterol (VLDL-c) were determined using standard methods. Atherogenic indices, non HDL cholesterol (Non HDL-c), cardio risk ratio (CRR), atherogenic index of plasma (AIP), atherogenic coefficient (AC) and atherosclerosis index (AI) were calculated. It was analyzed statistically using SPSS version 23.0. Results: The mean values of HDL-c was significantly higher in the treated diabetic group when compared with untreated diabetic control (P<0.05) while TC, TG, LDL-c, VLDL-c, Non HDL-c, CRR, AIP, AC and AI were significantly lower in treated diabetics when compared to the untreated diabetic control (P<0.05). Also, blood mean levels of HDL-c were significantly lower in the diabetic groups (treated and untreated) when compared with non diabetic control (P<0.05) while TC, TG, LDL-c, VLDL-c, Non HDL-c, CRR, AIP, AC and AI were significantly higher in the diabetic groups (treated and untreated) when compared with non diabetic control (P<0.05). Conclusion: The study suggests that atherogenic indices can serve as predictive pointer for future cardiovascular event especially, when LDLc value is normal. Also hyperglycemia could cause significant alterations of lipids, but metformin treatment has showed not only hypoglycemic effect, but also anti-hyperlipidemic properties.

scholarly journals Rutin ameliorates carbon tetrachloride (CCl4)-induced hepatorenal toxicity and hypogonadism in male rats

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e7011 ◽  
Author(s):  
Hany Elsawy ◽  
Gehan M. Badr ◽  
Azza Sedky ◽  
Basem M. Abdallah ◽  
Abdullah M. Alzahrani ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Sperm Quality ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Serum Testosterone ◽  
Lipoprotein Cholesterol ◽  
Male Rats ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Serum Triglycerides

Rutin, a food derived-polyphenolic bioflavonoid, has been acknowledged for several health benefits. This study aims to explore the ameliorative effects of rutin against carbon tetrachloride (CCl4) toxicity in male rats. Adult male rats were given either CCl4 (30% in olive oil, 3 ml/kg b.w. intraperitoneally) alone or in combination with rutin (70 mg/kg intragastrically) twice a week for 4 weeks. Our data showed that rutin mitigated CCl4 hepatorenal damage, as indicated by diagnostic markers (i.e., transaminases, alkaline phosphatase, total bilirubin, total protein, albumin, urea, uric acid and creatinine), and histopathological findings. In addition, CCl4 induced profound elevation of free radical generation and oxidative stress, as evidenced by increasing lipid peroxidation and reducing catalase, superoxide dismutase and glutathione peroxidase activities in liver, kidney and testicular tissues; these effects were suppressed by coexposure with rutin. Moreover, the increase in the levels of serum triglycerides, cholesterol, low-density lipoprotein cholesterol, and very-low-density lipoprotein cholesterol induced by CCl4 was effectively counteracted by rutin. The decrease in the level of high-density lipoprotein cholesterol in the CCl4 group was also counteracted by rutin treatment. Interestingly, the decreased levels of hormonal mediators associated with sperm production, including serum testosterone, luteinizing hormone and follicle-stimulating hormone, and the impaired sperm quality induced by CCl4 were reversed by rutin. Data from the current study clearly demonstrated that rutin supplementation could at least partly overcome CCl4-induced hepatotoxicity, nephrotoxicity and reproductive toxicity by antioxidant and antidyslipidemic effects.

scholarly journals PENGARUH FRAKSINASI BUNCIS (PHASEOLUS VULGARIS L.) TERHADAP PENURUNAN KADAR KOLESTEROL TIKUS PUTIH (RATTUS NORVEGICUS L.) YANG HIPERKOLESTEROLEMIA

2020 ◽  
Vol 5 (1) ◽  
pp. 14-20
Author(s):  
Lilik Sulastri ◽  
◽  
Putri Syafalia ◽  
Achmad Fauzi Isa
Keyword(s):  
Phaseolus Vulgaris ◽  
Rattus Norvegicus ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density ◽  
Sprague Dawley ◽  
Phaseolus Vulgaris L

Hiperlipidemia merupakan suatu keadaan meningkatnya kadar lipid darah yang ditandai dengan meningkatnya kadar kolesterol total, Low Density Lipoprotein (LDL), dan trigliserida dalam darah yang melebihi batas normal. Sampai saat ini telah banyak obat yang digunakan untuk penanganan hiperlipidemia baik obat sintetik dan obat herbal. Salah satu obat herbal yang telah digunakan oleh masyarakat Indonesia adalah buncis (Phaseolus vulgaris L.). Tujuan penelitian ini adalah untuk mengetahui pengaruh beberapa ekstrak (n-heksan, etilasetat dan air) buah buncis terhadap penurunan kadar kolesterol tikus jantan galur Sprague Dawley yang telah diinduksi kuning telur puyuh, PTU 0,02% dan asupan pakan aterogenik selama 14 hari. Pengukuran kadar kolesterol dilakukan secara enzimatis dengan alat kolesterolmeter Easy Touch®. Hasil fraksinasi ekstrak etanol buncis dengan pelarut n-heksan, etil asetat, dan air diujikan selama 7 hari dengan dosis 50 mg/kg BB diperoleh fraksi air sebagai fraksi yang paling aktif menurunkan kadar kolesterol darah. Pada uji lanjutan fraksi air dilakukan terhadap 5 kelompok perlakuan (5x5 ekor), yaitu kelompok 1 (Na CMC 0,5%) sebagai kontrol negatif, kelompok 2 (simvastatin 0,18 mg/200 g BB) sebagai kontrol positif dan variasi dosis fraksi air 50 mg/kg BB, 100 mg/kg BB serta 150 mg/kg BB (kelompok 3, 4, dan 5). Selama 21 hari perlakuan tikus tetap diberikan pakan aterogenik dan pengukuran kadar kolesterol dilakukan pada hari ke 0, 14, 21, 28, dan 35. Hasil uji lanjutan menunjukkan bahwa fraksi air ekstrak buncis pada dosis 50 dan 100 mg/kg BB tidak memiliki perbedaan yang nyata dengan simvastatin, sedangkan pada dosis 150 mg/kg BB menunjukkan perbedaan yang nyata dangan simvastatin dalam menurunkan kadar kolesterol.

scholarly journals Can Millet Consumption Help Manage Hyperlipidemia and Obesity?: A Systematic Review and Meta-Analysis

2021 ◽  
Vol 8 ◽  
Author(s):  
Seetha Anitha ◽  
Rosemary Botha ◽  
Joanna Kane-Potaka ◽  
D. Ian Givens ◽  
Ananthan Rajendran ◽  
...  
Keyword(s):  
Systematic Review ◽  
Meta Analysis ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Lipid Levels ◽  
Low Density Lipoprotein Cholesterol ◽  
High Lipid ◽  
Atherosclerotic Cardiovascular Diseases

Many health benefits of millets (defined broadly to also include sorghum) have been advocated, including their roles in managing and preventing diabetes; however, the effects of millets on hyperlipidemia (high lipid levels) have been underrecognized. A systematic review and meta-analysis were conducted to collate available evidence of the impacts of millets consumption on lipid profile, namely total cholesterol (TC), triacylglycerol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), and very-low–density lipoprotein cholesterol (VLDL-C). The results from 19 studies showed that the consumption of millets for periods as short as 21 days to 4 months reduced levels of TC, triacylglycerol, LDL-C, and VLDL-C (p&lt;0.01) by 8.0, 9.5, 10 and 9.0%, respectively. Four studies demonstrated that millets consumption brought TC and triacylglycerol levels to the normal levels (&lt;200 and &lt;150 mg/dl, respectively). Furthermore, upon consumption of millet-based meals, there was a 6.0% increase in the HDL-C 4.0 and 5.0% reduction in systolic and diastolic blood pressure, and 7.0% reduction in body mass index (BMI). This evidence, leads us to conclude that consumption of millets reduces hyperlipidemia and hence hypertension, and raises the levels of HDL-C (good cholesterol), which can be beneficial for managing the associated risk of developing hypertension and atherosclerotic cardiovascular diseases in future.Systematic Review Registration: The protocol of this systematic review has been registered in the online registration platform called “research registry” with the unique identification number “reviewregistry1123.”

scholarly journals PENGARUH PEMBERIAN EKSTRAK BUNGA PEPAYA (Carica papaya L) TERHADAP KADAR HIGH DENSITY LIPOPROTEIN (HDL) DAN LOW DENSITY LIPOPROTEIN (LDL) DARAH TIKUS (Rattus norvegicus) GALUR Sprague dawley JANTAN YANG DIBERI DIET TINGGI LEMAK

2019 ◽  
Vol 6 (4) ◽  
pp. 247-256
Author(s):  
Dita Fitriani ◽  
Hetti Rusmini ◽  
Yuliana Wildani Marek
Keyword(s):  
Rattus Norvegicus ◽  
Carica Papaya ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Control Group ◽  
Low Density ◽  
Sprague Dawley ◽  
Control Group Design ◽  
Group Design ◽  
Post Test

Hiperlipidemia merupakan suatu keadaan patologis akibat kelainan metabolisme lipid yang ditandai dengan peningkatan fraksi lipid di dalam darah. Bunga pepaya mengandung senyawa yang mampu mempertahankan kadar kolesterol dalam batas normal karena mengandung senyawa flavanoid dan tanin yang berperan sebagai antioksidan. Pada penelitian ini digunakan tikus sebagai hewan coba untuk mengetahui pengaruh pemberian ekstrak bunga pepaya (Carica papaya L) terhadap kadar HDL dan LDL darah tikus yang diberi diet tinggi lemak. Penelitian ini menggunakan 25 ekor tikus Sprague dawley jantan berusia 12-16 minggu dengan berat 200-250 g, dengan pre and post test with control group design. Tikus dibagi menjadi 5 kelompok yaitu; kelompok tikus yang hanya diberi pakan standar (K), kelompok tikus yang diberi diet tinggi lemak (KN), kelompok tikus yang diberi diet tinggi lemak dan ekstrak bunga pepaya 31 mg/kgBB (P1), kelompok tikus yang diberi diet tinggi lemak dan ekstrak bunga pepaya 62 mg/kgBB (P2), dan kelompok tikus yang diberi diet tinggi lemak dan ekstrak bunga pepaya 125 mg/kgBB (P3). Hasil penelitian menunjukkan bahwa pemberian ekstrak bunga pepaya dengan dosis 31 mg/kgBB, 62 mg/kgBB dan 125 mg/kgBB mampu meningkatkan rerata kadar HDL dan menurunkan rerata kadar LDL darah tikus secara signifikan (p <0,05). Namun semakin besar dosis tidak berpengaruh terhadap kadar HDL dan LDL.

scholarly journals Silymarin Ameliorates Acrylamide-Induced Hyperlipidemic Cardiomyopathy in Male Rats

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
Ashraf M. Abdel-Moneim ◽  
Hany Elsawy ◽  
Abdullah M. Alzahrani ◽  
Awatef Ali ◽  
Omar Mahmoud
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Male Rats ◽  
Antioxidant Enzyme Activities ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol

Acrylamide (AA) is a well-known potent carcinogen and neurotoxin that has been recently linked to atherosclerotic pathogenesis. The present study is aimed at investigating the protective effect of silymarin (SIL) as an antioxidant against AA-induced hyperlipidemic cardiomyopathy in male rats. The obtained results showed that animals exposed to AA exhibited a significant increase in the levels of cardiac serum markers, serum total cholesterol, triglycerides, low-density lipoprotein cholesterol, and very-low-density lipoprotein cholesterol with a significant decrease in high-density lipoprotein cholesterol. Furthermore, AA intoxication significantly increased the malondialdehyde level (a hallmark of lipid peroxidation) and reduced antioxidant enzyme activities (i.e., superoxide dismutase, catalase, and glutathione peroxidase). SIL administration significantly attenuated all these biochemical perturbations in AA-treated rats, except for the decreased high-density lipoprotein cholesterol. Our results were confirmed by histopathological assessment of the myocardium. In conclusion, this study demonstrated a beneficial effect of SIL therapy in the prevention of AA-induced cardiotoxicity by reversing the redox stress and dyslipidemia in experimental animals.

Dynamic changes of the renin-angiotensin and associated systems in the rat after pharmacological and dietary interventions in vivo

2008 ◽  
Vol 35 (3) ◽  
pp. 330-340 ◽  
Author(s):  
Yoshinori Ochiai ◽  
Yi-Qiang Liang ◽  
Masakuni Serizawa ◽  
Norihiro Kato
Keyword(s):  
Mrna Level ◽  
Male Rats ◽  
Mrna Levels ◽  
Dynamic Changes ◽  
Salt Loading ◽  
Renin Angiotensin ◽  
High Lipid ◽  
High Lipid Diet ◽  
Lipid Diet

To address the multiplicity of the renin-angiotensin system (RAS) with particular interest in its local, synergistic regulation, we investigate dynamic changes of the RAS and associated systems in response to external stimuli in the rat. We tested influences of the RAS blockade (candesartan and enalapril), diuretics (hydrochlorothiazide), high lipid diet, and salt loading on tissue mRNA level of 12 principal genes. Under the hemodynamic conditions appropriately predetermined, we quantitatively evaluated mRNA level changes with and without each intervention in five organs–the brain, heart, kidney, liver, and adipose tissues–of male rats ( n = 5 each). A total of 250 tissues were examined by real-time PCR. Significant changes in mRNA level ( P < 0.05) were found in a drug-, diet- and tissue-specific manner. For instance, 29% of genes (14 out of 48 tissues showing detectable mRNA levels) were differentially regulated by candesartan and enalapril, although both drugs reduced blood pressure to similar extents. When the overall interactions among 12 genes were compared between interventions, the RAS and associated systems appeared to change in the opposite direction between candesartan and high lipid diet in the adipose tissue and between candesartan and salt loading in the heart. Enalapril, however, induced unique patterns of perturbation in the local RAS under the corresponding conditions. Thus, this study provides a fundamental picture of gene expression profile in vivo in the RAS and associated systems. In particular, our data highlight differential regulation between candesartan and enalapril, which may reflect the individual pharmacological properties regarding clinical implications.

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