scholarly journals DEVELOPMENT AND CHARACTERIZATION OF TOPICAL NANOPARTICULATE ANTIPSORIATIC POLYHERBAL CREAM

2020 ◽  
pp. 67-73
Author(s):  
SHADAB KHAN ◽  
GHAZALA MULLA ◽  
KIRAN BHISE
Keyword(s):  
Drug Loading ◽  
Lawsonia Inermis ◽  
Drug Diffusion ◽  
Cream Formulation ◽  
Diffusion Studies ◽  
Preclinical Trials ◽  
Ultraviolet Ray ◽  
Hacat Cell Lines

Objective: The lack of possible cure and associated disadvantages of allopathic medicines in the topical treatment of psoriasis has led to extensive research for anti-psoriatic activities of plant-based drugs. The present study was aimed to develop topical cream formulation containing the Nanostructured Lipid Carriers of Azadirachta indica leaves extract (AE), Lawsonia inermis leaves extract (LE) and fruit extract of Mallotus philippensis (ME) and assessing the antipsoriatic activity of prepared cream formulation. Methods: The Drug loaded NLCs were prepared via hot homogenization technique and incorporated into the water in oil (w/o) cream base that was prepared by the emulsification method. Evaluation of cream formulation included advanced preclinical trials using Rat Ultraviolet Ray-B photodermatitis and Mousetail method, anti-lipid peroxidation assay, nitric oxide scavenging activity, cytotoxicity and DNA fragmentation on HaCat cell lines to ascertain antipsoriatic potential and in vitro drug diffusion. Results: In spite of the low amount of the drug loading in NLCs their potency was much higher as displayed in Advanced Preclinical studies and cell line studies for psoriasis indicating the suitability of NLCs loaded creams for skin applications due to their various desirable effects on the skin. Occlusive properties of the prepared NLC on the skin provide an increase in drug penetration particularly via skin moisturization. The in vitro drug diffusion studies suggest the prolonged and almost complete release of AE, LE and ME from NLC based cream up to 24 h that was found to be 81.24±0.51%, 74.31±0.25% and 70.43±0.78% respectively. The literature survey supports the novelty of such topical anti-psoriatic polyherbal nanoparticulate cream. Conclusion: It can be concluded that the AE, LE and ME loaded NLC based cream can be used for prolonged topical delivery of drugs for the management of Psoriasis.

Development and Characterization of Quercetin Loaded Nanoparticle for Skin Cancer

2019 ◽  
Vol 32 (1) ◽  
pp. 1-6
Author(s):  
Nikita Verma
Keyword(s):  
Skin Cancer ◽  
Drug Loading ◽  
Quadratic Model ◽  
Precipitation Method ◽  
Dietary Flavonoid ◽  
Ultraviolet Ray ◽  
Average Size ◽  
Different Characteristics ◽  
Polymer Ratio

As a disease skin cancer has obtained different characteristics over the decades. Solar radiation that contains ultraviolet ray is the prime cause of skin cancer. In this present research, the nano-precipitation method was applied for preparing Quercetin loaded Nanoparticle (Qu-Nps) with much enhanced loading properties and improves incorporation of corresponding drugs. At the same time, the Quadratic model that takes help of the Response Surface Method was applied to observe the effects of some specific parameters maintained in the development of nanoparticle. Here, the sonication time was 20 min and delivery system F6 (with Drug: Polymer ratio of 1:45) provided optimum drug entrapment ability which is 70%. The optimized formulation for average size was almost 102.39 ±7.64 nm with zeta potential diameter averaging -28.43mV. Quercetin is a dietary flavonoid possessing multidimensional properties that is used in various other diseases including viral infection, bacterial infection, diabetes mellitus, and cancer. All outcomes support the view that Quercetin loaded nanoparticles (Qu-Nps) has high entrapment and drug loading abilities.

PREPARATION AND CHARACTERIZATION OF GLYCYRRHETINIC ACID-MODIFIED POLOXAMER 188/CHITOSAN NANOPARTICLES

NANO ◽  
2013 ◽  
Vol 08 (04) ◽  
pp. 1350042 ◽  
Author(s):  
JING WANG ◽  
LI GUO ◽  
LI FANG MA
Keyword(s):  
High Performance ◽  
Drug Loading ◽  
Chitosan Nanoparticles ◽  
Spherical Shape ◽  
Glycyrrhetinic Acid ◽  
Poloxamer 188 ◽  
H Nmr ◽  
Ft Ir

In this paper, we firstly synthesized glycyrrhetinic acid-modified double amino-terminated poloxamer 188 (GA–NH–POLO–NH–GA). The structure of the synthesized compound was confirmed by 1H-NMR and Fourier transform infrared (FT-IR) spectroscopy. Then the nanoparticles composed of GA–NH–POLO–NH–GA/chitosan (GA–NH–POLO–NH–GA/CTS) were prepared by an ionic gelation process. The characterization of the nanoparticles was measured by dynamic light scattering (DLS) and scanning electron microscope (SEM). The results showed that the nanoparticles were well dispersed with a spherical shape and the particle size was distributed between 100 nm and 300 nm. The cytotoxicity based on MTT assay against cells (QGY-7703 cells and L929 cells) showed that the nanoparticles had low toxicity and good biocompatibility. The encapsulation efficiency and drug loading of 5-fluorouracil-loaded nanoparticles (5-FU nanoparticles) were measured by high-performance liquid chromatography (HPLC) and fluorescence spectroscopy, ultraviolet-visible (UV-vis) absorbance. The encapsulation of 5-Fu-loaded CTS nanoparticles was 12.8% and the drug loading was 2.9%, while the encapsulation of 5-Fu-loaded GA–NH–POLO–NH–GA/CTS nanoparticles was 20.9% and the drug loading was 3.36%. The release profile showed that the GA–NH–POLO–NH–GA/CTS nanoparticles were available for sustained release of 5-Fu. The GA–NH–POLO–NH–GA/CTS nanoparticles have a higher affinity to the QGY-7703 cells, so indicated that the GA–NH–POLO–NH–GA/CTS nanoparticles have the capacity of liver-targeting in vitro.

scholarly journals Soybean lecithin stabilizes disulfiram nanosuspensions with a high drug-loading content: remarkably improved antitumor efficacy

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Haowen Li ◽  
Biao Liu ◽  
Hui Ao ◽  
Jingxin Fu ◽  
Yian Wang ◽  
...  
Keyword(s):  
Soybean Lecithin ◽  
Drug Loading ◽  
Antitumor Efficacy ◽  
Oral Drug ◽  
High Drug ◽  
Breast Cancer Chemotherapy ◽  
Intravenous Injections

AbstractDisulfiram (DSF) has been considered as “Repurposing drug” in cancer therapy in recent years based on its good antitumor efficacy. DSF is traditionally used as an oral drug in the treatment of alcoholism. To overcome its rapid degradation and instability, DSF nanosuspensions (DSF/SPC-NSps) were prepared using soybean lecithin (SPC) as a stabilizer of high drug-loaded content (44.36 ± 1.09%). Comprehensive characterization of the nanosuspensions was performed, and cell cytotoxicity, in vivo antitumor efficacy and biodistribution were studied. DSF/SPC-NSps, having a spherical appearance with particle size of 155 nm, could remain very stable in different physiological media, and sustained release. The in vitro MTT assay indicated that the cytotoxicity of DSF/SPC-NSps was enhanced remarkably compared to free DSF against the 4T1 cell line. The IC50 value decreased by 11-fold (1.23 vs. 13.93 μg/mL, p < 0.01). DSF/SPC-NSps groups administered via intravenous injections exhibited better antitumor efficacy compared to the commercial paclitaxel injection (PTX injection) and had a dose-dependent effect in vivo. Notably, DSF/SPC-NSps exhibited similar antitumor activity following oral administration as PTX administration via injection into a vein. These results suggest that the prepared nanosuspensions can be used as a stable delivery vehicle for disulfiram, which has potential application in breast cancer chemotherapy.

scholarly journals FORMULATION AND EVALUATION OF ANTIFUNGAL CREAM OF CHLORPHENESIN

2021 ◽  
pp. 76-81
Author(s):  
VEENA S. ◽  
SURINDER KAUR ◽  
GURURAJ KULKARNI
Keyword(s):  
Fungal Infections ◽  
Dosage Forms ◽  
Skin Infections ◽  
Topical Drug Delivery ◽  
Cream Formulation ◽  
Diffusion Studies ◽  
In Vitro Diffusion ◽  
Materials Used ◽  
Routes Of Drug Administration

Objective: The main aim of our research was to develop an Antifungal cream formulation consisting of Chlorphenesin for the treatment of Fungal infections. Topical route is the most suitable route for skin infections. Methods: The development of topical drug delivery systems designed to have systemic effects appears to be beneficial for a number of drugs on account of several advantages over conventional dosage forms(or) routes of drug administration. An Antifungal cream formulation consisting of Chlorphenesin was prepared. Results: The formulation was subjected to in vitro diffusion studies. Microbiological studies were performed to find out the safety of materials used in the formulation. Conclusion: The developed cream consisting of Cholrphnesin was found to be safe and effective for the treatment of fungal infection.

scholarly journals Formulation and Evaluation of Galantamine Hydrobromide Proniosome Gel for Alzheimer’s disease

2020 ◽  
Vol 10 (2-s) ◽  
pp. 68-74
Author(s):  
Mohammed Sarfaraz ◽  
Tanvi Goel ◽  
H. Doddayya
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Phase Separation ◽  
Oral Delivery ◽  
Tween 80 ◽  
Separation Method ◽  
Tween 20 ◽  
Drug Diffusion ◽  
Diffusion Studies

Galantamine hydrobromide is formulated in tablets and capsules prescribed through oral delivery for the treatment of Alzheimer’s disease. However, oral delivery of drugs can cause severe side effects such as nausea, vomiting, and gastrointestinal disturbance. In the present research work, Galantamine hydrobromide is formulated as proniosome gel by Coacervation phase separation method using different surfactants such as Tweens and Spans. Overall eight formulations were developed and evaluated for various parameters. The prepared gels were viewed by naked eye to observe the colour of gel. Microscopical observations of the gels showed vesicles of optimum size from 3.030 mm (P2) - 3.735 mm (P5). The gel also showed optimum rate of spontaneity in the range 9.60 mm3x1000 (P7) to 11.80 mm3x1000 (P4) and entrapment efficiency of vesicles in the range 66.15% (P5) to 86.92% (P3). The gels had pH in suitable range of skin (5.92-6.9). The in vitro drug diffusion studies revealed that the drug diffusion was affected by the various surfactants used. The rank order of surfactant effect on in-vitro drug diffusion was Tween 80 > Tween 60 > Tween 40 >Tween 20 > Span 80 > Span 60 > Span 40 > Span 20. The proniosomal gel containing Tween 80 showed maximum drug diffusion (99.24%) and the gel containing Span 20 showed minimum drug diffusion (71.74%). FT-IR studies of optimized proniosome gel P8 revealed the absence of any chemical interactions between drug and carriers used. Keywords: Galantamine hydrobromide, Proniosome gel, Coacervation phase separation method,   Surfactants, in vitro drug diffusion studies.

scholarly journals PREPARATION AND CHARACTERIZATION OF THYMOQUINONE NANOPARTICLES PEGYLATED AS DRUG DELIVERY SYSTEM

2021 ◽  
Author(s):  
A. Hasrawati ◽  
Irsan Rizaldi ◽  
Deisy Febrianti ◽  
A Mumtihanah Mursyid ◽  
Neneng Amelia Bakri
Keyword(s):  
Peer Review ◽  
Nigella Sativa ◽  
Drug Loading ◽  
Physicochemical Characterization ◽  
Entrapment Efficiency ◽  
Peg 6000 ◽  
Black Cumin ◽  
Main Component

Objective: Thymoquinone is a main component of Black Cumin (Nigella  sativa Linn.) with various pharmacological activities, but has poor stability and bioavailability. The purpose of this study was to carry out the preparation and characterization of timoquinone nanoparticles PEGylation. Methods: The Thymoquinone nanoparticles  (TQ-NP) were made with PEGylation using PEG 6000 with the concentrations on each preparation of 3 mM (A), 4 mM (B), and 5 mM (C) then were evaluated by the parameter of yield percentage Entrapment Efficiency (EE) and Drug Loading (DL), drug release, size and distribution particle, morphological analysis and Fourier Transform-Infrared spectrophotometer (FTIR). Results: Thymoquinone nanoparticle was PEGylated with PEG 6000  has the highest efficiency entrapment of 99.9718±0.029% in formula A, with the capacity of drug loading 0,66%. Formulation A release 99.9718±0.029% of Thymoquinone at 50 minutes. The morphological observations with Scanning Electron Microscope (SEM) showed spherical nanoparticles morphology.                           Peer Review History: Received 11 September  2020; Revised 5 Decembe; Accepted 3 January, Available online 15 January 2021 UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file:                           Comments of reviewer(s):         Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 8.0/10 Reviewer(s) detail: Dr. Evren Alğin Yapar, Turkish Medicines and Medical Devices Agency, Turkiye, [email protected] Dr. Sally A. El-Zahaby, Pharos University in Alexandria, Egypt, [email protected] Similar Articles: ABACAVIR LOADED NANOPARTICLES: PREPARATION, PHYSICOCHEMICAL CHARACTERIZATION AND IN VITRO EVALUATION LONG CHAIN POLYMERIC CARBOHYDRATE DEPENDENT NANOCOMPOSITES IN TISSUE ENGINEERING EFFECT OF PEGYLATED EDGE ACTIVATOR ON SPAN 60 BASED- NANOVESICLES: COMPARISON BETWEEN MYRJ 52 AND MYRJ 59

BOX–BEHNKEN METHOD TO OPTIMIZE LORNOXICAM PRONIOSOMES: PREPARATION, IN VITRO CHARACTERIZATION AND ANALGESIC ACTIVITY

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (06) ◽  
pp. 21-33
Author(s):  
K. Vijaya Sri ◽  
◽  
D. Sandhya ◽  
M. Manchala ◽  
R. S Dashamukhi
Keyword(s):  
Analgesic Effect ◽  
Entrapment Efficiency ◽  
Box Behnken Design ◽  
Factors Affecting ◽  
Drug Diffusion ◽  
Percutaneous Permeation ◽  
In Vitro Characterization ◽  
Diffusion Studies ◽  
Drug Entrapment Efficiency

The objective of present investigation was to develop and evaluation of proniosomes as the carrier of lornoxicam for topical delivery. lornoxicam-loaded proniosomes were prepared by coacervation phase separation method. The Box–Behnken design used in this study helped in identifying the factors affecting drug entrapment efficiency and drug diffusion. Proniosomes were evaluated for appearance, pH, viscosity, entrapment efficiency and in vitro drug diffusion studies. The optimized formulations were further evaluated to vesicle size, shape, zeta potential, percutaneous permeation and analgesic effect. The vesicles were found to be unilamellar, spherical in shape. The analgesic effect of lornoxicam proniosomal gel showed better therapeutic activity.

Preparation and Characterization of Cyclophosphamide-Loaded Chitosan Microspheres

2012 ◽  
Vol 621 ◽  
pp. 130-133
Author(s):  
Yi Lin Ding ◽  
Su Su Ding ◽  
Guo Fang Ding
Keyword(s):  
Drug Release ◽  
Anticancer Agent ◽  
Loading Rate ◽  
Drug Loading ◽  
Average Diameter ◽  
Uv Spectrophotometry ◽  
Chitosan Microspheres ◽  
Dialysis Bag

Chitosan microspheres were prepared by using a cross linking agent combined with an emulsion technique. Cyclophosphamide was loaded as an anticancer agent. Obtained microspheres were spherical and regular, with a smooth surface morphology, having an average diameter of 15.7±9.0μm. After preparation, the drug-loading rate and entrapment rate of cyclophosphamide was investigated by UV spectrophotometry. Drug release was tested in vitro in a dynamic dialysis system with a dialysis bag. The chitosan microspheres prepared were proved to have good drug release profiles.

scholarly journals Rapamycin-Loaded Polymeric Nanoparticles as an Advanced Formulation for Macrophage Targeting in Atherosclerosis

Pharmaceutics ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 503
Author(s):  
Emanuela Fabiola Craparo ◽  
Marta Cabibbo ◽  
Alice Conigliaro ◽  
Maria Magdalena Barreca ◽  
Teresa Musumeci ◽  
...  
Keyword(s):  
Polymeric Nanoparticles ◽  
Drug Loading ◽  
Mammalian Target Of Rapamycin ◽  
Amorphous State ◽  
Entrapment Efficiency ◽  
Polymeric Nanoparticle ◽  
Specific Accumulation ◽  
Cd36 Receptor

Recently, rapamycin (Rapa) represents a potential drug treatment to induce regression of atherosclerotic plaques; however, its use requires site-specific accumulation in the vessels involved in the formation of the plaques to avoid the systemic effects resulting from its indiscriminate biodistribution. In this work, a stable pharmaceutical formulation for Rapa was realized as a dried powder to be dispersed extemporaneously before administration. The latter was constituted by mannitol (Man) as an excipient and a Rapa-loaded polymeric nanoparticle carrier. These nanoparticles were obtained by nanoprecipitation and using as a starting polymeric material a polycaprolactone (PCL)/α,β-poly(N-2-hydroxyethyl)-dl-aspartamide (PHEA) graft copolymer. To obtain nanoparticles targeted to macrophages, an oxidized phospholipid with a high affinity for the CD36 receptor of macrophages, the 1-(palmitoyl)-2-(5-keto-6-octene-dioyl) phosphatidylcholine (KOdia-PC), was added to the starting organic phase. The chemical–physical and technological characterization of the obtained nanoparticles demonstrated that: both the drug loading (DL%) and the entrapment efficiency (EE%) entrapped drug are high; the entrapped drug is in the amorphous state, protected from degradation and slowly released from the polymeric matrix; and the KOdia-PC is on the nanoparticle surface (KP-Nano). The biological characterization demonstrated that both systems are quickly internalized by macrophages while maintaining the activity of the drug. In vitro studies demonstrated that the effect of KP-Nano Rapa-loaded, in reducing the amount of the Phospo-Ser757-ULK1 protein through the inhibition of the mammalian target of rapamycin (mTOR), is comparable to that of the free drug.

Preparation and characterization of pH-responsive sodium alginate/humic acid/konjac hydrogel for L-ascorbic acid controlled release

2019 ◽  
Vol 9 (6) ◽  
pp. 563-569 ◽  
Author(s):  
Yuhua Niu ◽  
Tong Yang ◽  
Ruyuan Ke ◽  
Chen Wang
Keyword(s):  
Humic Acid ◽  
Sodium Alginate ◽  
Drug Loading ◽  
Ph Responsive ◽  
Swelling Properties ◽  
Ph Conditions ◽  
Konjac Gum ◽  
Scanning Electron

In the current work, a mixed hydrogel of sodium alginate (SA), humic acid (HA) and konjac gum (KGM) was studied by ionic coordination of Ca2+. The SA/HA/KGM hydrogel was characterized by scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The ratio of SA/HA/KGM to the amount of Ca2+ was investigated by the swelling property of the gel. The pH sensitivity of the SA/HA/KGM gel was investigated using the swelling properties of the gel under different pH conditions. In vitro drug release indicated that the formed SA/HA/KGM hydrogel had a good release effect on L-AA in different pH environments, and the encapsulation and drug loading of the drug were greatly improved.

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