scholarly journals ISOLATION AND PURIFICATION OF APIGENIN FROM ALLIUM FISTULOSUM

2020 ◽  
pp. 67-71
Author(s):  
TEENA MAGLINE IMMACULATE V. ◽  
SHLINI P. ◽  
MARY CLARE H.
Keyword(s):  
Amino Acids ◽  
Plant Extract ◽  
Cardiac Glycosides ◽  
Reducing Sugars ◽  
Allium Fistulosum ◽  
Phytochemical Screening ◽  
Isolation And Purification ◽  
Minor Peak ◽  
Phytochemical Constituents ◽  
First Time

Objective: The objective of this study is to optimize the extraction of apigenin (4′, 5, 7-trihydroxy-flavone) from Allium fistulosum. Methods: The present study aims at extracting flavonoids from the plant using methanol as a solvent. Phytochemical screening was performed to analyse the phytochemical constituents present in the extract. Apigenin was isolated from the leaves of A. fistulosum and further purified and identified by TLC and HPLC. Results: Phytochemical screening showed the presence of various constituents like terpenoids, tannins, flavonoids, reducing sugars, phenolics, cardiac glycosides and amino acids. TLC results indicated the presence of apigenin in the plant extract with the Rf value of 0.88, which coincided with the standard apigenin of Rf value 0.83. HPLC results showed a similarity in the peak of 17.1 min of the standard with the minor peak of 17.324 min of the sample. This proved the presence of apigenin in low quantities. Conclusion: This present study suggests that Allium fistulosum can be a moderate source of apigenin. This compound was isolated for the first time from this plant.

scholarly journals PHYTOCHEMICAL CONSTITUENTS OF THE ROOT OF BERBERIS MICROPHYLLA

2017 ◽  
Vol 10 (6) ◽  
pp. 225 ◽  
Author(s):  
María Cristina Furrianca ◽  
Marysol Alvear ◽  
Tomás Zambrano ◽  
Víctor Fajardo ◽  
Luis Salazar
Keyword(s):  
Secondary Metabolites ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
Medicinal Properties ◽  
Phytochemical Constituents ◽  
First Time

Objective: To objective of this work was to perform phytochemical qualitative and quantitative analyzes of the main secondary metabolites in the root of Berberis microphylla.Methods: The extracts of B. microphylla root were tested through phytochemical screening and the quantification of the most important constituents was carried out using spectrophotometric and gravimetric techniques.Results: Phytochemical screening of both extracts showed the presence of alkaloids, flavonoids, glycosides, cardiac glycosides, saponins, terpenes,and tannins, which are pharmacologically important. Quantification of the major phytochemicals groups showed that the ethanolic extract contains 3.9% alkaloids, 0.46% flavonoids, 9.53% tannins, and 3.60% saponins. Similarly, the methanolic extract contains 6.61% alkaloids, 0.41% flavonoids, 7.40% tannins, and 1.43% saponins.Conclusion: This is the first time that the presence of tannins, flavonoids, and saponins in this plant has been reported. The medicinal properties of the root of B. microphylla may exist due to the presence secondary metabolites.

Preliminary phytochemical screening, isolation and structural elucidation of chloroform leaf extracts of Maesa lanceolata

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Cardiac Glycosides ◽  
Leaf Extract ◽  
Chloroform Extract ◽  
Phytochemical Analysis ◽  
Spectroscopic Methods ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Standard Procedures ◽  
Phytochemical Constituents ◽  
First Time

The aim of this study was to screen the phytochemical constituents, isolate and elucidate the structure of chloroform extracts from the leaves of Maesa lanceolata. The qualitative phytochemical analysis of the leaf extract of Maesa lanceolata was done following standard procedures and the tests revealed the presence of cardiac glycosides and saponins in the crude extracts. The chloroform extract of the leaves of M. lanceolata led to the isolation of one compound, C1. Structure determination was accomplished by means of spectroscopic methods (IR, 13C and 1H NMR). According to the spectral data (IR, 1HNMR, 13CNMR and DEPT), probably Myrsenene was identified which is reported for the first time from M. lanceolata.

scholarly journals Phytochemical Screening and Biological Studies of Shilajit (Asphaltum)

2017 ◽  
Vol 9 (1) ◽  
pp. 15
Author(s):  
Shahid Aziz ◽  
Sidra Khaliq ◽  
Habib Ur-Rehman ◽  
Kh. Shakeel Ghani ◽  
Muhammad Irshad ◽  
...  
Keyword(s):  
Microbial Activity ◽  
Cardiac Glycosides ◽  
Reducing Sugars ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Biological Studies ◽  
Dead Plant ◽  
Micro Organisms

<p>Shilajit (asphaltum)  is produced  by the long term humification  of dead plant material  and organic vegetable matter  by different micro-organisms and has great potential for the treatment of a variety of human conditions.  This treatise reviews its origin, sources, chemical composition, biological  and  commercial importance. Phytochemical analysis was done by standard methods to evaluate  different Shilajit (asphaltum) classes of compounds in different samples of shilajit  which are responsible for their  biological activity.  Shilajit`s anti-microbial activity has been evaluated  against four different bacterial strains viz., <em>Escherichia coli, Psuedomonas aeuroginosa</em>, <em>Klebisella pneumonia</em> and <em>Staphylococcus aureus. </em>Phytochemical analysis illustrated  that shilajit contains  terpenoids, cardiac glycosides, saponins and reducing sugars. Surprisingly,  some classes of compounds are absent in shilajit  viz., alkaloids, flavonoids, tannins and anthraquinones. . Shilajit showed no response towards halophytic bacteria and  negligible activity was shown towards other strains of bacteria. Since   anti-microbial activity is based on environmental factors  its activity varied  between locations.</p>

scholarly journals Quantitative and Qualitative Identification of Phytochemical Constituents of Sida Rhombifolia Leaves Extract

2019 ◽  
Vol 8 (2S4) ◽  
pp. 403-408
Keyword(s):  
Quantitative Analysis ◽  
Cardiac Glycosides ◽  
Total Content ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Biological Compounds ◽  
Potential Sources ◽  
Pharmaceutical Industries ◽  
Phytochemical Constituents ◽  
Sida Rhombifolia

Anti-inflammatory, analgesic, antimicrobial, anti-arthritic, antibacterial, antispasmodic, hypoglycaemic and hepatoprotective characteristics of Sida rhombifolia are included in the Malvaceae family. Most of the plant belongs to the Malvaceae family, as they comprise a number of phytochemicals and biological compounds, are potential sources of different medications. This is study on Sida rhombifolia leaf extract's tophytochemical constituen ts. Phytochemical screening results in herbal stan-dardizat ion and preparation and may relate the components to thei r medicinal / pharmacological uses. The qualitative phytochemical analysis has shown that the extract is positive for saponins, flavonoids, alkaloids, phenols and same extract is negative for carbohydrate, tannins, glycosides, cardiac glycosides, terpenoids, coumarins, steroid& phytosteroids, phlobutanins, anthraquinones. Quantitative analysis of phytochemicals includes the estimation of flavonoid, tannin and total content of phenol. The result suggest that the Sida rhombifolia leaves extract consist plenty of phytochemicals beneficial in alternative medical and pharmaceutical industries.

scholarly journals Acute Toxicity and Anti-diarrhoeal Activity of Aqueous Extract of Aerial ‎Parts of Hygrophila Auriculata in Albino Rats

2020 ◽  
Vol 17 (2) ◽  
pp. 38-44
Author(s):  
S. I. Salihu ◽  
A. Telta ◽  
M. Chiroma ◽  
N. Daniel ◽  
C. Yakubu ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Plant Extract ◽  
Cardiac Glycosides ◽  
Standard Procedure ◽  
Scientific Basis ◽  
Albino Rats ◽  
Traditional Use ◽  
Aerial Parts ◽  
Intestinal Contents ◽  
Phytochemical Constituents

Hygrophila auriculata (H. auricalata) plant extract was studied for it phytochemical constituents, acute toxicity and its anti-diarrhoea ‎l activity in albino rats using standard procedure. The phytochemical screening revealed the presence of cardiac glycosides, terpenoids and saponins. The acute toxicity of the extract was above 2000 mg/kg b. wt which is slightly toxic. The result of castor oil induced diarrhoea ‎ model indicates that the extract at all test doses was significant (p<0.05). Similarly, the extract produced a significant (p<0.05) decline in the weight and volume of intestinal contents at all tested doses. In addition, a significant (p<0.05) reduction in the gastrointestinal motility in charcoal meal test was also observed in all doses of the extract administered. This activity may be attributed to the presence of the identified phytchemicals in the plant extract. The results in this study confirmed the ‎anti-diarrhoea. ‎ ‎l activity of the aerial part of H. auriculata and hence support the folkloric believe and provide the scientific basis for the traditional use of this plant in the treatment of diarrhoea.

scholarly journals Phytochemical Screening for Antioxidant Properties of Germinated Foxtail Millet

2019 ◽  
pp. 1-6 ◽  
Author(s):  
Jelang Jelku D. Sangma ◽  
W. Jessie Suneetha ◽  
B. Anila Kumari ◽  
K. B. Suneetha Devi
Keyword(s):  
Amino Acids ◽  
Cardiac Glycosides ◽  
Foxtail Millet ◽  
Antioxidant Properties ◽  
Phytochemical Screening ◽  
Human Diet ◽  
Plant Kingdom ◽  
Complex Group

Phytochemicals are a complex group of bioactive non-nutrient compounds of the plant kingdom and an integral part of the human diet. The present research was carried out to screen the phytochemicals in raw and germinated foxtail millets. The screening showed the presence of carbohydrates, alkaloids, fixed oils and fats, terpenoids and cardiac glycosides in both raw and germinated foxtail millets. Amino acids were not detected in raw but present in germinated, indicating in prominent in the bio-availability of amino-acids due to germination. The absence of quinones indicated that extracts have not undergone any oxidation during storage.

scholarly journals Phytochemical Screening and Mineral Composition of the Leaves of Ocimum gratissimum (Scent Leaf)

2016 ◽  
Vol 4 (2) ◽  
pp. 161-165 ◽  
Author(s):  
Priscilla Alexander
Keyword(s):  
Atomic Absorption ◽  
Elemental Analysis ◽  
Cardiac Glycosides ◽  
Chemical Components ◽  
Phytochemical Screening ◽  
Medicinal Uses ◽  
Standard Methods ◽  
Ocimum Gratissimum ◽  
Nutritive Values ◽  
Phytochemical Constituents

The leaves of Ocimum gratissimum having some ethno-medicinal applications were investigated. The phytochemicals screening of the leaves using Standard Methods and further analysis with HPLC revealed the presence of tannins, saponins, flavonoids, alkaloids, phlobatanins, terpenoids, steroids and cardiac glycosides. The elemental analysis was carried out using Atomic Absorption Spectrophotometer (AAS). The result revealed the presence of Na (0.311±0.049), Ca (0.138 ±0.111), Mg (1.712 ± 0.537), K (0.261± 0.077), Mn (0.457±0.107, Pb (0.005 ± 0.002), Zn (0.200 ±0.06), Cu (0.803±0.818), N (0.286± 0.052), and Fe (0.312 ± 0.067).  Cd and Cr were not detected in the samples. This investigation suggested that Ocimum gratissimum leaves are rich in phytochemical constituents which contributed to its medicinal uses. The leaves of Ocimum gratissimum can be said to contain some components of medical value since the chemical components elaborated by it are active principles (alkaloid, steroids and glycosides). Ocimum gratissimum also contain most of the essentials elements indicating its nutritive values and less toxic as Cd and Cr were not detected in the leaf.Int J Appl Sci Biotechnol, Vol 4(2): 161-165

scholarly journals Preliminary Study and Phytochemical Screening of Arum dioscorides Sibth. in Syria

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Ayoubi Ahmad Mohieddin ◽  
Al-Kurdi Khalil ◽  
Kattah Abdullah ◽  
Trefi Saleh
Keyword(s):  
Amino Acids ◽  
Extraction Method ◽  
Cardiac Glycosides ◽  
Room Temperature ◽  
Aqueous Ethanol ◽  
Cyanogenic Glycosides ◽  
Phytochemical Screening ◽  
Yield Increase ◽  
Significant Yield ◽  
Preliminary Study

Raphides (calcium oxalate needles) were detected in both of Arum dioscorides Sibth. et Sm. leaves and rhizomes. The extraction method of the leaves was optimized, in order to choose the most suitable solvent and method. The best solvent was the following mixture: ethanol, water, hexane, chloroform, acetone 38:16:25:9:12 v/v respectively. In addition, the second best solvent was aqueous ethanol 65%. While for the extraction method itself, sonication aided extraction at room temperature gave significantly better yields than maceration for 72 hours. Moreover, and the suitable time for sonicationaided extraction is 20 minutes. Because there was no significant yield increase through time prolongation after 20 min. Qualitative phytochemical screening of both the leaves and rhizomes was carried out indicating the presence of these phytochemicals in both of leaves and rhizomes: alkaloids, amino acids, phenolic acids, carbohydrates, coumarines, flavonoids, lignans, saponins, sterols, tannins, triterpinoids, pro anthocyanidin, catchins and traces of cyanogenic glycosides. While it has shown the absence of anthranoids and cardiac glycosides.

scholarly journals PHARMACOGNOSTIC AND PHYTOCHEMICAL SCREENING OF HELICTERES ISORA ROOTS

2016 ◽  
pp. 96 ◽  
Author(s):  
Veena Sharma ◽  
Urmila Chaudhary
Keyword(s):  
Amino Acids ◽  
Wound Healing ◽  
Moisture Content ◽  
Cardiac Glycosides ◽  
Water Soluble ◽  
Phytochemical Screening ◽  
Microbial Infection ◽  
Active Constituents ◽  
Standard Procedures ◽  
Helicteres Isora

<p>ABSTRACT<br />Objective: Helicteres isora Linn. roots have been reported to use in microbial infection, diabetes, dysentery colic, scabies, wound healing, and<br />gastropathy. The main objective of this study was preliminary phytochemical screening of various fractions of H. isora roots.<br />Methods: In this study, pharmacognostic and phytochemical investigations on the various fractions of H. isora roots were carried out with the<br />standard procedures.<br />Results: The pharmacognostic analysis revealed moisture content (0.18%), total ash (4%), acid insoluble ash (1%), and water soluble ash (1.5%). The<br />qualitative phytochemical screening revealed the presence of alkaloids, amino acids, proteins, carbohydrates, flavonoids, cardiac glycosides, saponin,<br />steroids, terpenoids, and tannins.<br />Conclusion: H. isora roots are a rich source of various biological active constituents. H. isora may be further investigated for the isolation of bioactive<br />compound that could be developed into drug for the treatment of various human diseases.<br />Keywords: Helicteres isora, Pharmacognostic, Phytochemical, Roots.</p><p> </p>

Phytochemical Isolation and Biological Activities of Corydalis adiantifolia from Baltistan

2019 ◽  
Vol 41 (3) ◽  
pp. 535-535
Author(s):  
Iftikhar Ali Iftikhar Ali ◽  
Zulfiqar Ali Zulfiqar Ali ◽  
Alishba Alishba ◽  
Zamarrud Zamarrud ◽  
Hidayat Hussain and Viqar Uddin Ahmad Hidayat Hussain and Viqar Uddin Ahmad
Keyword(s):  
Plant Material ◽  
Reducing Sugars ◽  
Methanol Extract ◽  
Biological Activities ◽  
Spectroscopic Methods ◽  
Phytochemical Screening ◽  
Whole Plant ◽  
Hela Cell Lines ◽  
Water Fractions ◽  
First Time

The whole plant material of Corydalis adiantifolia Hook.f. andamp; Thomson was investigated for biological activities e.g. antifungal, antibacterial, insecticidal, cytotoxic, anticancer and phytotoxic activities and preliminary phytochemical screening. The methanol extract and dichloromethane fraction of the plant species exhibited 5% inhibition each against the fungus Aspergillus flavus. The methanol extract and water and dichloromethane fractions exhibited non-significant antibacterial activity and they showed inhibition against Hela cell lines and insignificant insecticidal activity. To understand the bioactive profile of C. adiantifolia, phytochemical screening approach is considered effective. The samples including methanol extract and n-hexane, dichloromethane, ethyl acetate and water fractions were subjected to qualitative phytochemical screening for the presence of various phytochemicals i.e. alkaloids, flavonoids, saponins, diterprenes, triterpenoids, anthraquinones, anthranol glycosides, reducing sugars and phenols. The results exhibited the efficacy of methanol extract showing the presence of more phytochemicals in comparison to the fractions of C. adiantifolia. Moreover, as a result of phytochemical isolation, 8-acetonyldihydrosanguinarine (1), β-sitosterol (2) and β-sitosterol-β-D-glucoside (3) were isolated, purified, and characterized by spectroscopic methods. To the best of our knowledge, all this study was carried out for the first time on C. adiantifolia.

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