scholarly journals BIODEGRADABLE PLASTIC (PHB) FROM BACTERIA COLLECTED FROM GOPALPUR BEACH, BHUBANESWAR

2020 ◽  
pp. 41-44
Author(s):  
DIPALI KUMARI ◽  
DIPTI SONI JAIPURIAR ◽  
PARIJATHAM KANCHANA ◽  
SIBYALA MALATHI VARMA ◽  
SONY GURJA
Keyword(s):  
Positive Result ◽  
Water Sample ◽  
Biodegradable Polymer ◽  
Biodegradable Plastics ◽  
Environmental Concerns ◽  
Contaminated Water ◽  
Biological Applications ◽  
Bacterial Isolates ◽  
Reserve Material ◽  
Polyhydroxy Butyrate

Objective: Environmental concerns have prompted research into the development of biodegradable polymer as an alternative to petroleum-based plastics. Polyhydroxybutyrates (PHBs) are good alternatives to produce biodegradable plastics. Some bacteria are found to be producing PHB. The aim of this work was to isolate potential PHB producing bacteria and screen them for their ability to produce PHB. Methods: Contaminated water sample from Gopalpur beach, Bhubaneswar was the source of bacteria. Three colonies were isolated from the water sample. The samples were named as C1, C2 and C3. The colonies were first identified by colony morphology. The sudan black screening test was done to screen for the production of (PHB) polyhydroxy butyrate by bacterial isolates. Results: The bacterial isolates C1 and C2 showed a positive result for the production of polyhydroxy butyrate (PHB). Presence of PHB granules in Cocobacillus and Rod shaped bacillus was confirmed. Conclusion: Polyhydroxybutyrate (PHB), a kind of microbial polyester that accumulates as a carbon/energy reserve material in various microorganisms, was thus concluded to be a decent alternative for plastics. Because of their special characteristics and broad biological applications, biodegradable plastics are compounds with a promising future.

Organophosphorus compounds biodegradation by novel bacterial isolates and their potential application in bioremediation of contaminated water

2020 ◽  
Vol 317 ◽  
pp. 124003 ◽  
Author(s):  
Julia Yamila Santillan ◽  
Natalia Lorena Rojas ◽  
Pablo Daniel Ghiringhelli ◽  
Matías Leonardo Nóbile ◽  
Elizabeth Sandra Lewkowicz ◽  
...  
Keyword(s):  
Potential Application ◽  
Organophosphorus Compounds ◽  
Contaminated Water ◽  
Bacterial Isolates

scholarly journals Study On The Performance Of Carbonate-Mineralized Bacteria Combined With Eggshell For Immobilizing Pb and Cd in Water And Soil

2021 ◽  
Author(s):  
Ting Wei ◽  
Noman Yashir ◽  
Fengqiu An ◽  
Syed Asad Imtiaz ◽  
Xian Li ◽  
...  
Keyword(s):  
Cation Exchange Capacity ◽  
Exchange Capacity ◽  
Contaminated Water ◽  
Carbonate Precipitation ◽  
Bacterial Isolates ◽  
X Ray Diffraction ◽  
X Ray ◽  
Soil Enzymatic Activity ◽  
Microbially Induced Carbonate Precipitation ◽  
Eggshell Waste

Abstract Microbially induced carbonate precipitation (MICP) is an advanced bioremediation approach to remediate heavy metals (HMs) contaminated water and soil. In this study, metal tolerant urease-producing bacterial isolates, namely UR1, UR16, UR20 and UR21, were selected based on their urease activity. The efficiency of these isolates in water for Pb and Cd immobilization was explored. Our results revealed that UR21 had the highest removal rates of Pb (81.9%) and Cd (65.0%) in solution within 72 h through MICP. The scanning electron microscopy-energy dispersive x-ray and x-ray diffraction analysis confirmed the structure and the existence of PbCO3 and CdCO3 crystals in the precipitates. In addition, the strain UR21, in combination with urea/eggshell waste (EGS) or both, was further employed to investigate the effect of MICP on soil enzymatic activity, chemical fractions and bioavailability of Pb and Cd. The outcomes indicated that the applied treatments reduced the proportion of soluble-exchangeable Pb and Cd, resulted an increment in carbonated bound Pb and Cd in the soil. The DTPA extractable Pb and Cd was reduced by 29.2% and 25.2% with the treatment of UR21 + urea + EGS as compared to the control. Besides, the application of UR21 and EGS significantly increased the soil pH, cation exchange capacity, and enzyme activities. Our findings may provide a novel perceptive for an eco-friendly and sustainable approach to remediate heavy metal contaminated environment through a combination of metal-resistant ureolytic bacterial strain and EGS.

scholarly journals The Effects of Enrichment Nutrients on the Growth of Indigenous Bacteria Species in Spent Lubricating Oil Contaminated Water

2020 ◽  
pp. 39-48
Author(s):  
Tudararo-Aherobo Laurelta
Keyword(s):  
Bacterial Species ◽  
Organic Wastes ◽  
Lubricating Oil ◽  
Contaminated Water ◽  
Cow Dung ◽  
Pseudomonas Sp ◽  
Bacterial Isolates ◽  
Mineral Salts ◽  
Bacterial Counts ◽  
Significant Difference

Aim: This study was conducted to compare the effects of enrichment nutrients, NPK (Nitrogen, Posphorus, Potassium) and organic wastes on the growth of indigenous bacterial species in spent lubricating oil contaminated water. Six bacterial species which were isolated from spent lubricating oil impacted soils (Pseudomonas sp., Bacillus sp., Actinomyces sp., Acinetobacter sp., Enterobacter sp., and Micrococcus sp.,) and showed profuse utilization of spent lubricating oil on screening, were used for this study. Place and Duration of Study: The study was conducted in the laboratory of the Department of Environmental Management and Toxicology, Federal University of Petroleum Resources, Effurun, between 2018 and 2019. Methodology: The study and was conducted using Mineral Salts Medium broth, spent lubricating oil substrate and NPK (20:10:10), Chicken droppings and Cow dung as nutrient sources (biostimulants). The effect of the biostimulants on the growth of the bacterial isolates was assessed weekly for 14 days by measuring the turbidity, bacterial counts and pH. Results: Pseudomonas sp. recorded the highest count of 1.16E+19 CFU/ml, 2.53E+17 CFU/ml and 1.74E+14 CFU/ml for biostimulation with NPK, Chicken droppings and Cow dungs respectively. The treatment with NPK enhanced the bacterial isolates most, of the three treatments used at the end of the test period. The pH values obtained for the test cultures at the end of the study, ranged from 6.52±0.02 for Enterobacter sp. in Cow dung treated cultures to7.85±0.03 for Pseudomonas sp.in NPK treated cultures. The values were within the optimum biodegradation range of 6.50 -8.50. There was significant difference between the bacterial counts obtained with the cultures treated with NPK and Chicken droppings (P=0.006), between NPK and cow dungs (P = 0.031) and between NPK and the control (P = 0.033). The study affirms the benefits of using organic wastes in the bioremediation process of hydrocarbon contaminated sites; it enhances the nutrients required by the bacteria for the remediation process and it’s a waste management strategy for disposing these organic wastes at very minimal costs and in an ecofriendly manner.

Current Research in Biodegradable Plastics

2014 ◽  
Vol 679 ◽  
pp. 273-280 ◽  
Author(s):  
N.A. Faris ◽  
N.Z. Noriman ◽  
S.T. Sam ◽  
C.M. Ruzaidi ◽  
M.F. Omar ◽  
...  
Keyword(s):  
Biodegradable Polymer ◽  
Natural Fiber ◽  
Synthetic Polymers ◽  
Biodegradable Plastics ◽  
Fiber Reinforced ◽  
Renewable Sources ◽  
Reinforced Plastics ◽  
Fiber Reinforced Plastics ◽  
Packaging Industry

Synthetic polymers are important in many branches of industry, particularly in the packaging industry. However, it has an undesirable influence on the environment and causes problems with deposition of waste and consumption. Therefore, there is a tendency to replace the polymer with biodegradable polymer that undergoes a process. This review summarizes the data on consumption, the level of biodegradation, the reliability of commercialization and production from renewable sources. Some biodegradable plastics that have been commercialized are starch based plastics, bacteria based plastics, soy based plastics, cellulose based plastics, lignin based plastics and natural fiber reinforced plastics. Production of this kind of material and its introduction to the market is important for the natural environmental.

scholarly journals Microbacterium petrolearium sp. nov., isolated from an oil-contaminated water sample

2014 ◽  
Vol 64 (Pt_12) ◽  
pp. 4168-4172 ◽  
Author(s):  
Huimin Wang ◽  
Tingsheng Xiang ◽  
Yanwei Wang ◽  
Jinlong Song ◽  
Yi Zhai ◽  
...  
Keyword(s):  
Cell Wall ◽  
Water Sample ◽  
Type Species ◽  
Contaminated Water ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Pr China

A Gram-staining-positive, rod-shaped, aerobic bacterium, designated strain LAM0410T, was isolated from an oil-contaminated water sample from the Dagang Oilfield, PR China. The temperature and pH ranges for growth were 4 °C to 50 °C and pH 4.4 to pH 12.0, respectively. The strain did not need NaCl for growth, but could tolerate a concentration of up to 12 % (w/v). Cell wall hydrolysates from the isolate showed that the diamino acid was ornithine. The cell wall sugars contained ribose and galactose. The glycan moiety of the cell wall contained N-glycolyl residues. The major respiratory quinones were MK-10, MK-11 and MK-12. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and nine unknown glycolipids. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. 16S rRNA gene sequence analysis indicated that the isolate belonged to the genus Microbacterium and was closely related to Microbacterium sediminis MCCC 1A06153T and Microbacterium murale DSM 22178T with 97.5 % and 97.4 % sequence similarities, respectively; while the DNA–DNA hybridization values were 33.1±3.4 % and 21.8±1.6 %, respectively. The DNA G+C content was 67.4 mol%, as determined by the T m method. Based on its phenotypic and genotypic properties, strain LAM0410T represents a novel species of the genus Microbacterium , for which the name Microbacterium petrolearium sp. nov. is proposed; the type strain is LAM0410T ( = ACCC 00719T = JCM 19612T).

scholarly journals Copper Contamination of Different Prawn Farms at Shatkhira District

2012 ◽  
Vol 4 (2) ◽  
pp. 105-109
Author(s):  
ML Ali ◽  
MA Sattar ◽  
MA Baten
Keyword(s):  
Natural Resources ◽  
Water Sample ◽  
Copper Concentration ◽  
Contaminated Water ◽  
Central Laboratory ◽  
Copper Contamination ◽  
Allowable Limit ◽  
Sediment Samples ◽  
Cu Contamination ◽  
Mean Concentration

Copper (Cu) contamination of six prawn farms under three upazilas of Satkhira district were evaluated. Total 54 water, sediment and prawn samples were collected from six farms, 18 samples from each category were examined to observe the Cu contamination of water, sediment and prawn. Cu was determined by Atomic Absorption Spectrophotometer at the central laboratory of Bangladesh Agricultural University and toxicity laboratory of Bangladesh Fisheries Research Institute, Mymensingh. The levels of Cu in water sample, sediment sample and samples of prawn species were collected from different surface and ground sources. The concentration of Cu in sediment samples were ranged from 45.3895 to 127.8771 ?g-1 and the average mean concentration was 76.92101 ?g-1. The concentration of Cu in prawn was ranged from 16.1069 to 97.3841 ?g-1 and the average mean concentration was 51.2342 ?g-1. The copper concentration in water ranged from 0.0309 to 0.0702 ppm. Cu concentrations in those samples were higher in sediment than prawn, but very lower concentration was found in water sample. The Cu in sediment samples were present higher amount than allowable limit. So prawn was mostly affected by contaminated water and sediment.DOI: http://dx.doi.org/10.3329/jesnr.v4i2.10157  J. Environ. Sci. & Natural Resources, 4(2): 105-109, 2011

scholarly journals Immobilization of cesium-resistant bacterial cells by radiation polymerization and their bioremoval efficiency

2019 ◽  
Vol 79 (8) ◽  
pp. 1587-1596 ◽  
Author(s):  
Rita Singh ◽  
Kirti Shitiz ◽  
Antaryami Singh
Keyword(s):  
Immobilized Cells ◽  
Absorption Capacity ◽  
Contaminated Water ◽  
Bacterial Cells ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Radiation Polymerization ◽  
Polymer Carriers ◽  
Removal Of Heavy Metals ◽  
Different Doses

Abstract Biological approaches for the removal of heavy metals and radionuclides from contaminated water are reported. The present study was carried out with the objective of identifying bacterial strains for the uptake of cesium that could be used for bioremediation. Polymer carriers prepared by radiation polymerization were used for the immobilization of bacteria and the efficiency of free cells and immobilized cells for the removal of cesium was evaluated. Thirty-five bacterial isolates were screened for resistance to cesium and five bacterial isolates based on resistance to cesium (BR-3, BR-6, BR-21, BR-39, BR-40) were selected for immobilization. Polymer carriers were prepared using 10, 20, 30, 40 and 50% acrylamide at different doses of 1 to 5 kGy gamma radiation. The polymer carriers prepared using 30% and 40% acrylamide at 5 kGy were found to be suitable based on gel fraction and absorption capacity for the immobilization of bacterial cells. Bioremoval of cesium by free and immobilized bacterial cells was evaluated. Significant reductions of 76–81% cesium were observed with bacterial cells immobilized by radiation polymerization.

Arsenic Removal by Precipitation with Calcium Phosphate Hydroxyapatite

2012 ◽  
Vol 506 ◽  
pp. 413-416 ◽  
Author(s):  
W. Dungkaew ◽  
K.J. Haller ◽  
A.E. Flood ◽  
J.F. Scamehorn
Keyword(s):  
Calcium Phosphate ◽  
Water Sample ◽  
Removal Efficiency ◽  
Arsenic Removal ◽  
Arsenic Speciation ◽  
Arsenic Concentration ◽  
Contaminated Water ◽  
Low Levels ◽  
Removal Of Arsenic ◽  
Key Parameter

The removal of arsenic from synthetic arsenic contaminated water sample by precipitating arsenic (in the form of arsenate oxyanion) with calcium phosphate hydroxyapatite, HAp, was studied under conditions that induce arsenate incorporated calcium phosphate hydroxyapatite, Ca (P/As)HAp, to form. Arsenate is able to substitute for a fraction of the phosphate in HAp host material as it forms. Consequently, arsenic is successfully removed from the contaminated water achieving up to 99% arsenic removal from 25 ppm initial arsenic concentration. The Ca:(P+As) and P:As mole ratios were found to play an important role in arsenic removal efficiency. Higher Ca:(P+As) and P:As mole ratios give higher arsenic removal efficiency. Surprisingly, the pH of the initial anion solution, a key parameter in arsenic speciation, was found to not have a significant effect on arsenic removal by this process. The advantage of this process is that the precipitation can occur rapidly at relatively low levels of arsenic contamination, implying an easy and inexpensive process for arsenic removal can be developed based on this approach.

scholarly journals Zeaxanthin Biosynthesis by Members of the Genus Muricauda

2014 ◽  
Vol 63 (1) ◽  
pp. 115-119 ◽  
Author(s):  
SUDHARSHAN PRABHU ◽  
P.D. REKHA ◽  
A.B. ARUN
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Health ◽  
High Performance ◽  
Biological Applications ◽  
Bacterial Isolates ◽  
Marine Broth ◽  
First Report ◽  
Carotenoid Composition

Zeaxanthin, a C40xanthophyll carotenoid, has potential biological applications in nutrition and human health. In this study we characterized carotenoid composition in 5 taxonomically related marine bacterial isolates from the genus Muricauda. The pigment was characterized using high performance liquid chromatography (HPLC) and mass spectrometry, which confirmed the presence of all-trans-zeaxanthin. Muricauda strains produced zeaxanthin as a predominant carotenoid. M. flavescens JCM 11812(T) produced highest yield (4.4 +/- 0.2 mg L(-1)) when cultured on marine broth at 32 degrees C for 72 h. This is the first report on the presence of zeaxanthin among the majority of species from the genus Muricauda.

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