scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING, TOTAL PHENOL AND FLAVONOID CONTENT OF MIMOSA RUBICAULIS AND REINWARDITA INDICA

2019 ◽  
pp. 54-58
Author(s):  
ROSHANI GURUNG
Keyword(s):  
Plant Species ◽  
Biological Effects ◽  
Total Phenol ◽  
Aluminium Chloride ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Specific Chemical ◽  
Dry Extract

Objective: Phytochemicals as phenol and flavonoid have a powerful biological activity. So, this study aimed to carry out phytochemical screening, total phenol and flavonoid content in two plant species i.e. M. rubicaulis and R. indica. Methods: The extraction of different parts of two plant species was done by maceration using ethanol. Phytochemical screening was done to confirm the presence of phytochemicals. Total phenol content was done by Folin ciocalteu method and total flavonoid content was done by Aluminium chloride colorimetric method. Results: Phytochemical analysis revealed the presence of flavonoid, phenol, terpenoids in both plant species. The highest concentration of phenol content was observed in the root and stem of an extract of M. rubicaulis i.e. 281.83±1.98 mg GAE/g dry extract weight and 225.37±0.60 mg GAE/g dry extract weight. The highest concentration of flavonoid contents was observed in the leaves of R. indica i.e. 462.21±4.67 mg QE/g dry extract weight followed by stem and root of M. rubicaulis i.e. 381.06±5.23 mg QE/g dry extract weight and 337.43±1.39 mg QE/g dry extract weight. Conclusion: Phytochemical analysis concluded the presence of biologically important phytoconstituents like flavonoid and phenol in both plant species. Further studies, should be carried out to isolate specific chemical constituents and should be used in different studies to explore their biological effects.

scholarly journals Antioxidant properties of Rubus discolor leaf extracts

2016 ◽  
pp. 189-196 ◽  
Author(s):  
Ivona Velickovic ◽  
Slavica Grujic ◽  
Petar Marin
Keyword(s):  
Antioxidant Properties ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Aqueous Extracts ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
Dry Extract

In this work were examined aqueous, methanol, ethanol and acetone leaf extracts of Rubus discolor, wild growing blackberry, for their antioxidant properties and total phenol and flavonoid content. The total phenol content (TPC) varried from 250.05 to 446.61 mg GAE/g of dry extract, while total flavonoid content (TFC) was in range between 22.44 and 61.15 mg QE/g of dry extract. Aqueous extracts were the richest in phenols, as well as in flavonoids. In vitro antioxidant capacity of leaf extracts was evaluated by 2,2-diphenyl-1-picrylhydrazil (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) free radical scavenging procedures and ferric reducing ability of plasma (FRAP) assay. Aqueous extracts were the most effective through all antioxidant tests. The total phenol content highly correlated with antioxidant activity of extracts. Moreover, weak correlation was established between total phenol and total flavonoid content. The results presented in this work indicate that phenol compounds contribute to antioxidant ability of extracts.

scholarly journals Study of total phenol, flavonoid contents and phytochemical screening of methanolic crude extracts of two weed plants

2017 ◽  
Vol 6 (06) ◽  
pp. 1645 ◽  
Author(s):  
Shah Shreshtha ◽  
Jain Anushi ◽  
Ambika Nitesh Joshi* ◽  
Nitesh Joshi ◽  
Harshal Anupma
Keyword(s):  
Large Scale ◽  
Total Phenol ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Antimicrobial Drugs ◽  
Alternanthera Sessilis ◽  
Ipomoea Carnea ◽  
Continuous Use

Weeds have been neglected and their use for medicinal purpose has not been considered on a large scale. Our current research was based on the phytochemical Screening of Ipomoea carnea and Alternanthera sessilis and to quantitatively evaluate their total phenolic and flavonoid content. Quantitative analysis of Phenols and Flavonoids was done by the Folin Ciocalteau and Aluminium chloride method respectively. Both weeds showed positive response for presence of secondary metabolites like flavonoids, phenols, tannins, terpenoids, proteins, carbohydrates, etc. which indicates that they can be used as herbal components. The total Phenol value of Ipomoea carnea and Alternanthera sessilis was 0.0526 and 0.0657 mg Gallic acid equivalent/g respectively. The flavonoid content value of Ipomoea carnea and Alternanthera sessilis was 0.09 and 0.2 mg quercetin equivalent (QE) /g respectively. Both the weeds showed presence of several phytochemicals and high Phenol and Flavonoid values. However, Alternanthera showed a higher concentration of Phenols and Flavonoids as compared to Ipomoea. Since both the selected weeds grow in many areas throughout the world, their medicinal properties can be exploited and used for several applications. With the continuous use of antibiotics, microorganisms have become resistant. There is a need to develop alternative antimicrobial drugs using weeds, which otherwise have not been exploited till now by humans.

scholarly journals ASSESSMENT OF TOTAL PHENOL AND FLAVONOID CONTENT OF COSTUS SPECIOSUS AND CATHARANTHUS ROSEA IN DIFFERENT GEOGRAPHICAL REGIONS OF BALAGHAT DISTRICT, MADHYA PRADESH

2020 ◽  
Vol 8 (11) ◽  
pp. 1024-1029
Author(s):  
Rajeshwari Prabha Lahare ◽  
◽  
Harishankar Yadav ◽  
Keyword(s):  
Madhya Pradesh ◽  
Total Phenol ◽  
Aluminium Chloride ◽  
Total Phenol Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Pharmacological Activities ◽  
Costus Speciosus ◽  
Geographical Regions ◽  
Chloride Method

Phenols and flavonoids are secondary metabolites of the plant and play a key role in scavenging free radicals and act as antioxidant. Costus speciosus (Costaceae family) and Catharanthus rosea (Apocynaceae family) are important ornamental and ethno medicinal plant rich in bioactive compounds used for treatment of several types of diseases. The plants are well known for their various pharmacological activities such as anti-diabetic, anticancer, antibacterial, antifungal, antioxidant, anti-hyperglycemic, anti-inflammatory, analgesic, antipyretic, anti-diuretic, antiulcer and anti-diarrheal activities. In the present study the total phenol and flavonoid content of root methanol extract of both plants were determined from five blocks of Balaghat district Madhya Pradesh. Folin-Ciocalteau assay was used to determine total phenol content and aluminium chloride method for flavonoid content using gallic acid and quercetin as standard respectively. The result showed highest concentration of total phenol content in Birsa block for both C. speciosus (95.85±1.56 mg GAE/g) and C. rosea (82.32±0.81 mg GAE/g). The total flavonoid content was found highest in Paraswada block for C. speciosus (97.67±0.53mg/QE) and Baihar block for C. rosea (93.20±0.54mg/QE). The ratio of flavonoid to the phenolic in each extract was also determined to as certain extracts that are rich in flavonoids.

scholarly journals Antioxidant activity and total phenolic content in Caulerpa filiformis (Chlorophyta) from Sechura Bay and Paracas Bay, Peru

2020 ◽  
Vol 27 (1) ◽  
pp. 061-066 ◽  
Author(s):  
Joyce Mamani ◽  
Jorge Chávez ◽  
Eder Apumayta ◽  
Patricia Gil-Kodaka
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Methanolic Extract ◽  
Total Phenol ◽  
North Coast ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phytochemical Screening ◽  
Phenol Content ◽  
The North

In Peru, Caulerpa filiformis is a marine algae listed as an invasive species. For years, its distribution has been considered to be in the north coast (Isla Lobos de Afuera and Piura) until a recent report of its distribution in the central coast (Ancash, Lima, and Ica). The present investigation aims to determine the main groups of secondary metabolites, total phenol content, and antioxidant activity of the methanolic extract of C. filiformis from Sechura Bay (Piura) and Paracas Bay (Ica). The main chemical groups were determined through phytochemical screening, the content of phenols by the Folin–Ciocalteu method, and antioxidant activity by the ABTS method (2,2-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid]) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical screening of the methanolic extract of C. filiformis from Sechura Bay and Paracas Bay revealed the presence of carbohydrates, polyphenols, tannins, flavonoids, lipids, alkaloids, steroids, and triterpenes for both extracts. The total phenol content of the extract of C. filiformis from Sechura Bay (39.31 ± 0.39 mg of AGE/g extract) was significantly higher (p < 0.05) than that from Paracas Bay (18.78 ± 0.31 mg of AGE/g extract). In the ABTS and DPPH assays, the antioxidant capacity of the Sechura C. filiformis extract (IC50 = 3.49 ± 0.01 and 2.18 ± 0.02 mg/mL) was significantly higher (p < 0.05) than that of the Paracas C. filiformis extract (IC50 = 6.41 ± 0.02 and 2.42 ± 0.04 mg /mL). These findings suggest that the methanolic extract of C. filiformis is a source of secondary metabolites with an antioxidant potential.

scholarly journals Phytochemical Analysis and Biological Activities ofCola nitidaBark

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Durand Dah-Nouvlessounon ◽  
Hubert Adoukonou-Sagbadja ◽  
Nafan Diarrassouba ◽  
Haziz Sina ◽  
Adolphe Adjanohoun ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Biological Activities ◽  
Biological Effects ◽  
Meat Products ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Potato Dextrose Agar Medium ◽  
Free Radical Activity ◽  
Cola Nitida

Kola nut is chewed in many West African cultures and is used ceremonially. The aim of this study is to investigate some biological effects ofCola nitida’s bark after phytochemical screening. The bark was collected, dried, and then powdered for the phytochemical screening and extractions. Ethanol and ethyl acetate extracts ofC. nitidawere used in this study. The antibacterial activity was tested on ten reference strains and 28 meat isolatedStaphylococcusstrains by disc diffusion method. The antifungal activity of three fungal strains was determined on the Potato-Dextrose Agar medium mixed with the appropriate extract. The antioxidant activity was determined by DPPH and ABTS methods. Our data revealed the presence of various potent phytochemicals. For the reference and meat isolated strains, the inhibitory diameter zone was from17.5±0.7 mm (C. albicans) to9.5±0.7 mm (P. vulgaris). The MIC ranged from 0.312 mg/mL to 5.000 mg/mL and the MBC from 0.625 mg/mL to >20 mg/mL. The highest antifungal activity was observed withF. verticillioidesand the lowest one withP. citrinum. The two extracts have an excellent reducing free radical activity. The killing effect ofA. salinalarvae was perceptible at 1.04 mg/mL. The purified extracts ofCola nitida’s bark can be used to hold meat products and also like phytomedicine.

scholarly journals Aktivitas Antioksidan dan Evaluasi Sensoris Teh Herbal Bunga Gumitir (Tagetes erecta L.)

2020 ◽  
Vol 5 (2) ◽  
pp. 39
Author(s):  
I Gusti Ngurah Bagus Pranantha Bistara Kusuma ◽  
Ni Komang Ayu Nila Ratna ◽  
Auriel Gabriella Kalalinggi ◽  
I Wayan Rai Widarta
Keyword(s):  
Water Content ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Bioactive Components ◽  
Flavonoid Content ◽  
Herbal Tea ◽  
Tagetes Erecta L

Bunga gumitir memiliki manfaat yang baik bagi kesehatan karena mengandung komponen bioaktif berupa flavonoid, fenol, dan karotenoid berupa lutein sehingga sangat berpotensi digunakan sebagai teh herbal. Penelitian ini bertujuan untuk mendapatkan teknik pengeringan teh herbal bunga gumitir yang tepat sehingga menghasilkan karakteristik teh herbal bunga gumitir dengan komponen bioaktif dan antioksidan yang tinggi serta sensoris yang baik. Pengeringan dilakukan dengan 5 teknik yang berbeda yaitu pengeringan matahari, pengeringan oven, pengeringan dingin, pengeringan udara, dan pengeringan sangrai. Parameter yang diamati meliputi kadar air, total fenol, total flavonoid, aktivitas antioksidan (IC50) serta  pengujian  sensoris air seduhan teh herbal bunga gumitir (warna, aroma, rasa, dan penerimaan keseluruhan). Hasil penelitian menunjukkan bahwa pengeringan dingin mampu menghasilkan teh herbal dengan karakteristik terbaik yaitu kadar air sebesar 6,86%, total fenol sebesar 83,88 mg GAE/g ekstrak, total flavonoid sebesar 373,06 mg QE/g ekstrak, nilai IC50 sebesar 257,65 mg/L, karakteristik sensoris paling disukai dengan rasa agak khas bunga gumitir, rasa tidak asam, dan berwarna merah kekuningan.   Gumitir flower had good benefits for health because their bioactive components contain flavonoids, phenols, and carotenoids like lutein so that gumitir flower were potentially used as herbal tea. The research was conducted to obtain the appropriate gumitir flower tea drying technique to produced the characteristics of gumitir herbal tea with high bioactive and antioxidant components and good sensory. Drying were carried out with 5 different techniques, namely sun drying, oven drying, cold drying, air drying, and roasting drying. Parameters observed included water content, total phenol content, total flavonoid content, antioxidant activity (IC50), and sensory testing of gumitir flower herbal tea (color, aroma, taste, and overall reception). The results showed that cold drying was able to produced herbal tea with the best characteristics with water content was 6.86%, total phenol content was 83.88 mg GAE/g extract, total flavonoid content was 373.06 mg QE/g extract, IC50 was 257.65 mg/L, sensory characteristics were most preferred with a rather typical taste of gumitir flower, not sour and yellowish red.

scholarly journals Antioxidant Activities and Heavy Metal Contents of Castanea Sativa Honey

2018 ◽  
Vol 20 (3) ◽  
pp. 541-550 ◽  
Keyword(s):  
Heavy Metal ◽  
Castanea Sativa ◽  
Total Phenol ◽  
Total Flavonoid ◽  
Total Phenol Content ◽  
Scavenging Activity ◽  
Total Flavonoid Content ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Antioxidant Power

<p>Honey is one of the most valuable foods in terms of its antioxidant nature and antioxidant activity. In this study, their botanic origins, total flavonoid content, total phenol content, the hydrogen peroxide scavenging activity (HPSA) (in terms of SC50 (µg mL-1)), ferric reducing antioxidant power capacity (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (in terms of SC50), metal-chelating activity (MCA) (%) and heavy metal amounts were examined to determine the qualities of honey samples that gathered from Giresun city of Black Sea region in Turkey. According to the melissopalynological analysis, the botanic origins of the samples are Castanea sativa (Chestnut) Miller and uni-floral. The HPSA, FRAP, DPPH, MCA (%), total phenol content (TPC), and total flavonoid content (TFC) were found between 251.99±0.48-258.64±1.22 µg mL-1, 71.34±0.09-73.71±0.20 %, 584.86±0.06-595.04±0.29 µg mL-1, 36.73±0.00-36.86±0.09 %, 93.82±1.05-173.15±2.46 mg GAE 100 g-1 and 5.51±0.19-8.29±0.05 mg CAE/100 g, respectively. For comparison of these results, Butylated Hydroxy Anisole (BHA), Butylated Hydroxy Toluene (BHT) and α-Tocopherol (TOC) were used as standard antioxidant compounds. In addition, it was observed that honey samples were been contaminated with most of the heavy metals (Al, Ca, Cr, Cu, Fe, Li, Mg, Mn, Ni, Rb, and Zn) to a lesser or greater extent, whereas others (Te, Tl, and U) were never detected in these samples. However, Cd and Cs were detected only in one sample and Pb in another sample Finally, the results indicate that although honey is an important source of nutrients, nevertheless it could be affected by environmental pollution.</p>

scholarly journals SKRINING FITOKIMIA DAN PENETAPAN KADAR FENOL TOTAL PADA EKSTRAK DAUN NANGKA (Artocarpus heterophyllus), CEMPEDAK (Artocarpus integer), dan TARAP (Artocarpus odoratissimus) ASAL DESA PENGARON KABUPATEN BANJAR

2021 ◽  
Vol 4 (1) ◽  
pp. 95-102
Author(s):  
Muhammad Ikhwan Rizki ◽  
◽  
Nurlely Nurlely ◽  
Fadlilaturrahmah Fadlilaturrahmah ◽  
Ma’shumah Ma’shumah
Keyword(s):  
Abdominal Pain ◽  
Secondary Metabolites ◽  
Total Phenol ◽  
Total Phenol Content ◽  
Phytochemical Screening ◽  
Phenol Content ◽  
Artocarpus Heterophyllus ◽  
Phenol Group ◽  
The People ◽  
Leaf Powder

The leaves of jackfruit (Artocarpus heterophyllus), cempedak (Artocarpus integer), and tarap (Artocarpus odoratissimus) are used empirically by the people of Desa Pengaron, Banjar Regency for treat antimalarials, antidiabetic, abdominal pain, and cancer. The secondary metabolites of the phenol group are responsible for various plant activities. This study aims to determine of compounds and total phenol content in leaves of jackfruit (A. heterophyllus), cempedak (A. integer), and tarap (A. odoratissimus). The leaf samples were oven-dried, then the leaf powder was extracted using ethanol. Phytochemical screening was carried out on the extract using reagents, and phenol content was determined using a spectrophotometer UV-Vis. The results showed that the extracts of leaves jackfruit (A. heterophyllus), cempedak (A. integer), and tarap (A. odoratissimus) contained phenol, flavonoids, and tannins. Total phenol content of leaves A. heterophyllus, A. integer and A. odoratissimus were 13,174 ± 1,378 mgGAE / gram extract, 37,204 ± 2,202 mgGAE / gram extract, and 35,886 ± 0.890 mgGAE / gram extract.

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