scholarly journals Study of total phenol, flavonoid contents and phytochemical screening of methanolic crude extracts of two weed plants

2017 ◽  
Vol 6 (06) ◽  
pp. 1645 ◽  
Author(s):  
Shah Shreshtha ◽  
Jain Anushi ◽  
Ambika Nitesh Joshi* ◽  
Nitesh Joshi ◽  
Harshal Anupma
Keyword(s):  
Large Scale ◽  
Total Phenol ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Antimicrobial Drugs ◽  
Alternanthera Sessilis ◽  
Ipomoea Carnea ◽  
Continuous Use

Weeds have been neglected and their use for medicinal purpose has not been considered on a large scale. Our current research was based on the phytochemical Screening of Ipomoea carnea and Alternanthera sessilis and to quantitatively evaluate their total phenolic and flavonoid content. Quantitative analysis of Phenols and Flavonoids was done by the Folin Ciocalteau and Aluminium chloride method respectively. Both weeds showed positive response for presence of secondary metabolites like flavonoids, phenols, tannins, terpenoids, proteins, carbohydrates, etc. which indicates that they can be used as herbal components. The total Phenol value of Ipomoea carnea and Alternanthera sessilis was 0.0526 and 0.0657 mg Gallic acid equivalent/g respectively. The flavonoid content value of Ipomoea carnea and Alternanthera sessilis was 0.09 and 0.2 mg quercetin equivalent (QE) /g respectively. Both the weeds showed presence of several phytochemicals and high Phenol and Flavonoid values. However, Alternanthera showed a higher concentration of Phenols and Flavonoids as compared to Ipomoea. Since both the selected weeds grow in many areas throughout the world, their medicinal properties can be exploited and used for several applications. With the continuous use of antibiotics, microorganisms have become resistant. There is a need to develop alternative antimicrobial drugs using weeds, which otherwise have not been exploited till now by humans.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING, TOTAL PHENOL AND FLAVONOID CONTENT OF MIMOSA RUBICAULIS AND REINWARDITA INDICA

2019 ◽  
pp. 54-58
Author(s):  
ROSHANI GURUNG
Keyword(s):  
Plant Species ◽  
Biological Effects ◽  
Total Phenol ◽  
Aluminium Chloride ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Phenol Content ◽  
Flavonoid Content ◽  
Specific Chemical ◽  
Dry Extract

Objective: Phytochemicals as phenol and flavonoid have a powerful biological activity. So, this study aimed to carry out phytochemical screening, total phenol and flavonoid content in two plant species i.e. M. rubicaulis and R. indica. Methods: The extraction of different parts of two plant species was done by maceration using ethanol. Phytochemical screening was done to confirm the presence of phytochemicals. Total phenol content was done by Folin ciocalteu method and total flavonoid content was done by Aluminium chloride colorimetric method. Results: Phytochemical analysis revealed the presence of flavonoid, phenol, terpenoids in both plant species. The highest concentration of phenol content was observed in the root and stem of an extract of M. rubicaulis i.e. 281.83±1.98 mg GAE/g dry extract weight and 225.37±0.60 mg GAE/g dry extract weight. The highest concentration of flavonoid contents was observed in the leaves of R. indica i.e. 462.21±4.67 mg QE/g dry extract weight followed by stem and root of M. rubicaulis i.e. 381.06±5.23 mg QE/g dry extract weight and 337.43±1.39 mg QE/g dry extract weight. Conclusion: Phytochemical analysis concluded the presence of biologically important phytoconstituents like flavonoid and phenol in both plant species. Further studies, should be carried out to isolate specific chemical constituents and should be used in different studies to explore their biological effects.

scholarly journals Spectrophotometric determination of sun protective factor (SPF) and antioxidant potential of different solvent extracts of graptophyllum pictum

2019 ◽  
Vol 10 (SPL1) ◽  
Author(s):  
Noor Diyana Ibrahim ◽  
Wan Nur Farihah Che Mustafa ◽  
Mahendran Sekar ◽  
Khor Poh Yen ◽  
Seow Lay Jing
Keyword(s):  
Total Phenolic Content ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Total Flavonoid ◽  
Antioxidant Potential ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Uv Spectrophotometry ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Natural substances extracted from plants have been gaining attraction as protective agents due to their safety and responsible for multiple biological effects on skins. The present study was to determine the total phenolic and flavonoid contents, Sun Protection Factor (SPF), and antioxidant potential of different solvent extracts of Graptophyllum pictum leaves. The dried powdered leaves were extracted by the cold maceration method, using three different solvents, i.e., methanol, ethanol, and water. The extracted leaves was tested for antioxidant activity using of DPPH (1,1-diphenyl-2-picryl-hydrazyl). Total phenolic content was determined using the Follin-Ciocalteu and for total flavonoid content with used the aluminium chloride. The SPF of all extracts were analyzed by ultraviolet (UV) spectrophotometry. The total phenolic content is the highest in methanol extract (377.61±0.31 µg/ml), and total flavonoid content is the highest in ethanol extract (158.06 ± 0.18 µg/ml). For DPPH free radical scavenging activity, ethanolic extract showed the maximum percentage of inhibition, and for SPF, methanol extract had displayed the highest. The study recommends that methanol is a good solvent for the extraction of sun-protective constituents from G. pictum.

scholarly journals Evaluation of Antioxidant Potential of Stem and Leaf Extracts of Himalayan Tinospora cordifolia Hook.f. & Thomson

2021 ◽  
Vol 9 (1) ◽  
pp. 2-8
Author(s):  
Geetanjali Upadhyay ◽  
Lalit M. Tewari ◽  
Geeta Tewari ◽  
Neha Chopra ◽  
Naveen C. Pandey ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Leaf Extract ◽  
Radical Scavenging ◽  
Aluminium Chloride ◽  
Tinospora Cordifolia ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Leaf Extracts ◽  
The Family ◽  
Stem Extract

Background: Medicinal plants are considered a rich source of ingredients, which can be used in drug development and synthesis. Tinospora cordifolia (Wild.) Hook.f. & Thomson, commonly known as guduchi, heart-leaved moonseed and giloya is a herbaceous vine of the family Menispermaceae, has several beneficial properties including antioxidant activity. Aim: The present study was carried out to analyze the antioxidant activity of leaf and stem extracts of Tinospora cordifolia by using DPPH (1,1-Diphenyl-2-picrylhydrazyl) and ABTS (2,2´-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) free radical scavenging assays. Materials and Methods: Dried and powdered leaves and stem of T. cordifolia were extracted with methanol. Ascorbic acid was taken as standard. Total phenolic content was estimated by using Folin-ciocalteu's reagent while total flavonoid content by aluminium chloride reagent to find the correlation of polyphenols with antioxidant activity. ABTS assay of methanolic leaf and stem extracts showed the highest scavenging activity as compared to the DPPH assay. Results: Methanolic stem extract showed higher phenolic and flavonoid content along with antioxidant activity as compared to the methanolic leaf extract. Conclusion: The stem extract exhibited more antioxidant activity than the leaf extract with regards to the all parameters analyzed.

scholarly journals Antioxidant activity and total phenolic content in Caulerpa filiformis (Chlorophyta) from Sechura Bay and Paracas Bay, Peru

2020 ◽  
Vol 27 (1) ◽  
pp. 061-066 ◽  
Author(s):  
Joyce Mamani ◽  
Jorge Chávez ◽  
Eder Apumayta ◽  
Patricia Gil-Kodaka
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Methanolic Extract ◽  
Total Phenol ◽  
North Coast ◽  
Total Phenolic ◽  
Total Phenol Content ◽  
Phytochemical Screening ◽  
Phenol Content ◽  
The North

In Peru, Caulerpa filiformis is a marine algae listed as an invasive species. For years, its distribution has been considered to be in the north coast (Isla Lobos de Afuera and Piura) until a recent report of its distribution in the central coast (Ancash, Lima, and Ica). The present investigation aims to determine the main groups of secondary metabolites, total phenol content, and antioxidant activity of the methanolic extract of C. filiformis from Sechura Bay (Piura) and Paracas Bay (Ica). The main chemical groups were determined through phytochemical screening, the content of phenols by the Folin–Ciocalteu method, and antioxidant activity by the ABTS method (2,2-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid]) and 2,2′-diphenyl-1-picrylhydrazyl (DPPH). The phytochemical screening of the methanolic extract of C. filiformis from Sechura Bay and Paracas Bay revealed the presence of carbohydrates, polyphenols, tannins, flavonoids, lipids, alkaloids, steroids, and triterpenes for both extracts. The total phenol content of the extract of C. filiformis from Sechura Bay (39.31 ± 0.39 mg of AGE/g extract) was significantly higher (p < 0.05) than that from Paracas Bay (18.78 ± 0.31 mg of AGE/g extract). In the ABTS and DPPH assays, the antioxidant capacity of the Sechura C. filiformis extract (IC50 = 3.49 ± 0.01 and 2.18 ± 0.02 mg/mL) was significantly higher (p < 0.05) than that of the Paracas C. filiformis extract (IC50 = 6.41 ± 0.02 and 2.42 ± 0.04 mg /mL). These findings suggest that the methanolic extract of C. filiformis is a source of secondary metabolites with an antioxidant potential.

scholarly journals Antioxidant, Anti-Diabetic and Phytochemical Screening of Dried Ficus carica Linn Fruit Powder

2020 ◽  
Vol 2 (1) ◽  
pp. 50
Keyword(s):  
Ethyl Acetate ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Dpph Assay ◽  
Ic50 Value ◽  
Different Types

Ficus carica L. or the common name is red figs that belong to the Moraceae family or the Mulberry family. Every part of the fruit or the tree has been able to treat different types of common diseases, for example, it is used as a natural laxative or a supplementary food for diabetes. Thus, this research uses four different solvents, ethyl acetate, ethanol, hexane, and water, to obtain the respective crude extracts in order to investigate the anti-diabetic properties by determining the inhibition of the activity of the diabetic enzymes, α-amylase, and α-glucosidase; and the DPPH assay determines the antioxidant activity while qualitative phytochemical screening was for phenols, alkaloids, tannins, saponins, and flavonoids; total quantitative phenolic and total flavonoid content was done. The phytochemical screening showed the presence of the compounds tested in different types of crude extracts. For the total phenolic content, ethyl acetate extract exhibits the highest content. In contrast, hexane extract shows the highest total flavonoid content. For the DPPH assay, ethyl acetate extract has the highest scavenging activity at 13.351 µg/mL with corresponding with the data of total phenolic content. For the α-glucosidase inhibitory activity, water extract has the lowest IC50 value among the four extracts but higher value than the standard. For α-amylase inhibitory activity, only ethanol extract showed the IC50 value, but it was a high value. In conclusion, there is potential for figs to be a natural source of medicine, and the extracts tested can be used for future studies.

scholarly journals Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal

Plants ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 96 ◽  
Author(s):  
Sushant Aryal ◽  
Manoj Kumar Baniya ◽  
Krisha Danekhu ◽  
Puspa Kunwar ◽  
Roshani Gurung ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Wild Vegetables ◽  
Ic50 Value

Eight selected wild vegetables from Nepal (Alternanthera sessilis, Basella alba, Cassia tora, Digera muricata, Ipomoea aquatica, Leucas cephalotes, Portulaca oleracea and Solanum nigrum) were investigated for their antioxidative potential using 2,2-dyphenyl-1-picrylhydrazyl (DPPH) scavenging, hydrogen peroxide (H2O2), ferric reducing antioxidant power (FRAP), and ferric thiocyanate (FTC) methods. Among the selected plant extracts C. tora displayed the highest DPPH radical scavenging activity with an IC50 value 9.898 μg/mL, whereas A. sessilis had the maximum H2O2 scavenging activity with an IC50 value 16.25 μg/mL—very close to that of ascorbic acid (16.26 μg/mL). C. tora showed the highest absorbance in the FRAP assay and the lowest lipid peroxidation in the FTC assay. A methanol extract of A. sessilis resulted in the greatest phenolic content (292.65 ± 0.42 mg gallic acid equivalent (GAE)/g) measured by the Folin–Ciocalteu reagent method, while the smallest content was recorded for B. alba (72.66 ± 0.46 GAE/g). The greatest flavonoid content was observed with extracts of P. oleracea (39.38 ± 0.57 mg quercetin equivalents (QE)/g) as measured by an aluminium chloride colorimetric method, while the least was recorded for I. aquatica (6.61 ± 0.42 QE/g). There was a strong correlation between antioxidant activity with total phenolic (DPPH, R2 = 0.75; H2O2, R2 = 0.71) and total flavonoid content (DPPH, R2 = 0.84; H2O2, R2 = 0.66). This study demonstrates that these wild edible leafy plants could be a potential source of natural antioxidants.

scholarly journals EVALUATION OF IN VITRO ANTIOXIDANT AND α-AMYLASE INHIBITORY ACTIVITY OF PHYLLANTHUS INDOFISCHERI BENNET

2016 ◽  
Vol 8 (11) ◽  
pp. 131 ◽  
Author(s):  
Kalpana S ◽  
Ramakrushna B. ◽  
Anitha S.
Keyword(s):  
Inhibitory Activity ◽  
Colorimetric Method ◽  
Radical Scavenging ◽  
Total Flavonoid ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Ic50 Values

Objective: The present study evaluates the antioxidant and α-amylase inhibitory activity of leaf and bark extracts of Phyllanthus indofischeri with methanol and water as solvents. In addition to this, the total phenolic content and total flavonoid content was determined.Methods: The total phenolic and total flavonoid content of the extracts was determined by folin ciocaletus reagent method and aluminium chloride colorimetric method respectively. The antioxidant and α-amylase inhibitory activity were measured by various assays, including α, α-diphenyl-ẞ-dipicryl-hydrazyl (DPPH) free radical scavenging, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulphonicacid) (ABTS) radical scavenging, superoxide radical scavenging, total antioxidant capacity by phosphomolybdate method and porcine pancreatic α-amylase inhibitory assay. The IC50 values were calculated and compared with standards such as gallic acid, ascorbic acid and α-acarbose.Results: The results illustrated that all the extracts of Phyllanthus indofischeri exhibit significant antioxidant and α-amylase inhibitory activity. Among the extracts, methanolic leaf extract showed high levels of activity followed by bark water extract.Conclusion: Phyllanthus indofischeri extracts had shown antioxidant and α-amylase inhibitory activity. On the basis of these results, Phyllanthus indofischeri can be used as a natural antioxidant and hypoglycemic agent against various disorders related to oxidative stress; and the isolation of bioactive compounds was warranted. 

scholarly journals TOTAL PHENOLIC AND FLAVONOID CONTENT, ANTIOXIDANT ACTIVITY AND ANTIMICROBIAL POTENTIAL OF PHLOMIS PUNGENS WILLD

10.23856/3713 ◽  
2020 ◽  
Vol 37 (6) ◽  
pp. 133-139
Author(s):  
Yehor Bazavluk ◽  
Vira Hamada ◽  
Nataliya Polish ◽  
Roksolana Konechna ◽  
Solomiia Mykytiuk ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Colorimetric Assay ◽  
Total Content ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Natural Plant ◽  
Ethanol Extracts ◽  
Antioxidant Effects ◽  
Phenolic And Flavonoid

The objective of the present study was to determine the total content of phenolics and flavonoids in ethanol extracts of Phlomis рungens Willd. The total phenolic content was estimated spectrophotometrically using Folin Ciocalteu method. The total flavonoid content was measured by aluminium chloride colorimetric assay. The antioxidant and antimicrobial effects of the extracts were investigated. The Phlomis рungens Willd can be regarded as a promising  natural plant source of antimicrobial and antioxidant effects with high potential for phytopreparations.

scholarly journals Total Phenolic and Flavonoid Content, Antioxidant, and Toxicity Test with BSLT of Meistera chinensis Fruit Fraction from Southeast Sulawesi

2021 ◽  
Vol 4 (1) ◽  
pp. 6-15
Author(s):  
Musdalipah Musdalipah ◽  
Selfyana Austin Tee ◽  
Karmilah Karmilah ◽  
Sahidin Sahidin ◽  
Adryan Fristiohady ◽  
...  
Keyword(s):  
Toxicity Test ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Strong Activity ◽  
Local Plant ◽  
Chemical Content ◽  
Vacuum Liquid Chromatography ◽  
Phenolic And Flavonoid

Meistera chinensis is one of the new generations of the Zingiberaceae family. Zingiberaceae have a large number of species and still need research to reveal their chemical content. Meistera chinensis, a local plant that is widespread in Konawe Regency, Southeast Sulawesi. Empirically, M. chinensis is used as a flavor enhancer in food, aches, and increases body immunity. There are no reports of chemical content or its biological activity. In this study, the fruit was tested for phytochemicals, antioxidants using the ABTS test, total phenolic, total flavonoid content, and toxicity test with BSLT. Meistera chinensis was extracted with ethanol and fractionated by ethyl acetate solvent use vacuum liquid chromatography. Phytochemical screening was carried out qualitatively by using the calorimetric method. The fraction toxicity was monitored by a lethal test for brine shrimp (BSLT). These fractions for the ABTS method obtained fractions 1-8 (F1-F8) and ascorbic acid were used as controls. The results showed radical scavenging activities fraction of M. chinensis fruit was a very strong activity with IC50 of 42.7±3.53 mg/L (F8). The total phenolic and flavonoid contents were 30.72±1.07 mgGAE/g and 8.02±0.48 mgQE/g, respectively. The phytochemical evaluation contains terpenoids, saponins, phenolics, steroids, alkaloids, and flavonoids. The BSLT toxicity test was found to be very toxic with IC50 of 5.20±0.72 mg/L. These findings indicate that the fruit of M. chinensis acts as an antioxidant and toxicity agent.

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