scholarly journals EVALUATION OF ANTIDIABETIC ACTIVITY OF ZANTHOXYLUM OVALIFOLIUM LEAF EXTRACTS

2021 ◽  
pp. 56-60
Author(s):  
ARUN K. ◽  
VIRUPAKSHA J. H.
Keyword(s):  
Lipid Profile ◽  
Aqueous Extract ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Diabetic Rat ◽  
High Dose ◽  
Leaf Extracts ◽  
Standard Drug ◽  
Histopathological Studies

Objective: The present study was aimed to investigate the antidiabetic activity of ethanolic and aqueous extract of Zanthoxylum ovalifolium on alloxan induced diabetic rat model in rats. Methods: The leaves of Zanthoxylum ovalifolium were evaluated for antidiabetic activity by using alloxan induced diabetic model in diabetic rats. Diabetes was induced by single intraperitoneal injection of alloxan (100 mg/kg) and rats were treated orally with test extracts, standard drug (glibenclamide 5 mg/kg) and vehicle for 21 d. The hypoglycemic effects and lipid profile of diabetic rats were assessed using diagnostic kits. Finally, histopathological studies were carried out for pancreas. Results: The acute toxicity studies revealed at the dose of 2000 mg/kg (b. w) of Zanthoxylum ovalifolium for ethanol and aqueous extract were found to be safe. A significant reduction (p<0.001) in blood glucose was observed in diabetic rats treated with different doses of extracts compared to untreated diabetic rats. The drug possesses a good hyperlipidemic effect by normalizing the lipid parameters. This was evidenced by histopathological studies; both glibenclamide and 400 mg/kg of Ethanolic extract does appear to be regulated diabetes at the cellular level, resulting in the restoration of near normal architecture pancreatic islet of langerhans. Conclusion: It can be concluded from our research findings that ethanolic and aqueous extract of Zanthoxylum ovalifoliumat high dose (400 mg/kg) exhibited significant antihyperglycemic activity than extract at low dose (200 mg/kg) in alloxan induced diabetic rats. These extracts also showed improvement in parameters like lipid profile as well as regeneration β-cells in the pancreas and so might be of value in diabetes treatment.

scholarly journals Hypoglicemic and Antioxidant Activity of Petiveria alliacea in Diabetic Rat Models

2020 ◽  
Vol 3 (1) ◽  
pp. 19
Author(s):  
Vania Azalia Gunawan ◽  
Harlina Soetjipto ◽  
Arifa Mustika
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
The Body ◽  
Antidiabetic Activity ◽  
Diabetic Rat ◽  
High Dose ◽  
Leaf Extracts ◽  
Chronic Hyperglycemia ◽  
Petiveria Alliacea

Introduction: Diabetes mellitus is a degenerative disease characterized by chronic hyperglycemia conditions in the body. Various complications of diabetes mellitus are caused by oxidative stress condition. Petiveria alliacea (P. alliacea) is a potential plant and easy to grow in hot regions. Leaf extracts of P. alliacea contain flavonoids and tannins which work as antidiabetic and antioxidant. In addition, other compounds found in P. alliacea leaf extracts like linoleic acid and allantoin show an increase in insulin secretion. Therefore, this study aimed to determine the antidiabetic activity of ethanolic extract of P. alliacea. Methods: We investigated the hypoglycemic and antioxidant effect of P. alliacea on STZ-induced diabetic rats. Rats were randomly divided into six groups named normal control, diabetes control, metformin (150 mg/kg/d), low dose of P. alliacea (90 mg/kg/d), intermediate dose (180 mg/kg/d), and high dose (360 mg/kg/d). Rats were orally given the treatment daily in the morning for fourteen days. At the end of the study, blood glucose level was measured and rats were sacrificed to measure blood malondialdehyde level.malondialdehydeResults: P. alliacea extract dose of 90 mg/kg and 360 mg/kg, and also metformin significantly decrease blood glucose levels. P. alliacea extract dose of 360 mg/kg was able to lower blood malondialdehyde level significantly which were not obtained on metformin. Conclusion: This finding suggests that ethanolic extract of P. alliacea possess antidiabetic effect at least on rats.

scholarly journals ANTIDIABETIC EFFECT OF MIKANIA SCANDENS ON ALLOXAN-INDUCED RAT MODEL

2018 ◽  
Vol 11 (3) ◽  
pp. 109
Author(s):  
Rimashree Baishya ◽  
Anjan Adhikari ◽  
Sharmistha Biswas ◽  
Soma Banerjee
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Standard Drug ◽  
Glucose Levels ◽  
Male Wistar Rats ◽  
Histopathological Studies ◽  
Alloxan Monohydrate

 Objective: The objective was to study the evaluation of antidiabetic and antioxidant activity of different doses ethanolic extract of Mikania scandens leaves.Methods: The rats were divided into five groups of six animals each (non-diabetic control, diabetic control, standard drug-treated, and plant treated groups (250 and 500 mg/kg body weight)). Diabetes was induced in the healthy male Wistar rats (150–200 g body weight, 4–6 weeks old) by the administration of alloxan monohydrate (150 mg/kg, i.p.). On the 0th, 3th, 7th, 11th, and 15th days, the blood samples were analyzed for blood glucose. The antioxidant and antidiabetic parameters were evaluated by standard protocol. The liver tissue was used for histopathological assessment of liver damage.Results: The ethanolic extract of leaves of M. scandens showed a significant reduction (p<0.001) in the blood glucose levels and the antioxidant levels, with 500 mg/kg of body weight in the alloxan-induced diabetic rats as compared to the controls. Histopathological studies provided evidence that the leaf extract possesses antidiabetic activity.Conclusions: The ethanolic extract of leaves of M. scandens showed potent antidiabetic and antioxidant abilities against alloxan-induced rats, which indicate the presence of hypoglycemic constituents in the plant.

scholarly journals Phytochemicals of Periploca aphylla Dcne. ameliorated streptozotocin-induced diabetes in rat

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Umbreen Rashid ◽  
Muhammad Rashid Khan
Keyword(s):  
Hematological Parameters ◽  
Local Population ◽  
Diabetic Rats ◽  
Total Soluble Protein ◽  
Antidiabetic Activity ◽  
Diabetic Rat ◽  
Diabetic Patients ◽  
Sprague Dawley ◽  
Dna Damages ◽  
Standard Drug

Abstract Background Periploca aphylla is used by local population and indigenous medicine practitioners as stomachic, tonic, antitumor, antiulcer, and for treatment of inflammatory disorders. The aim of this study was to evaluate antidiabetic effect of the extract of P. aphylla and to investigate antioxidant and hypolipidemic activity in streptozotocin (STZ)-induced diabetic rats. Methods The present research was conducted to evaluate the antihyperglycemic potential of methanol extract of P. aphylla (PAM) and subfractions n-hexane (PAH), chloroform (PAC), ethyl acetate (PAE), n-butanol (PAB), and aqueous (PAA) in glucose-overloaded hyperglycemic Sprague-Dawley rats. Based on the efficacy, PAB (200 mg/kg and 400 mg/kg) was tested for its antidiabetic activity in STZ-induced diabetic rats. Diabetes was induced via intraperitoneal injection of STZ (55 mg/kg) in rat. Blood glucose values were taken weekly. HPLC-DAD analysis of PAB was carried out for the presence of various polyphenols. Results HPLC-DAD analysis of PAB recorded the presence of rutin, catechin, caffeic acid, and myricetin. Oral administration of PAB at doses of 200 and 400 mg/kg for 21 days significantly restored (P < 0.01) body weight (%) and relative liver and relative kidney weight of diabetic rats. Diabetic control rats showed significant elevation (P < 0.01) of AST, ALT, ALP, LDH, total cholesterol, triglycerides, LDL, creatinine, total bilirubin, and BUN while reduced (P < 0.01) level of glucose, total protein, albumin, insulin, and HDL in serum. Count of blood cells and hematological parameters were altered in diabetic rats. Further, glutathione peroxidase, catalase, superoxide dismutase, glutathione reductase, and total soluble protein concentration decreased while concentration of thiobarbituric acid reactive substances and percent DNA damages increased (P < 0.01) in liver and renal tissues of diabetic rats. Histopathological damage scores increased in liver and kidney tissues of diabetic rats. Intake of PAB (400 mg/kg) resulted in significant improvement (P < 0.01) of above parameters, and results were comparable to that of standard drug glibenclamide. Conclusion The result suggests the antihyperglycemic, antioxidant, and anti-inflammatory activities of PAB treatment in STZ-compelled diabetic rat. PAB might be used as new therapeutic agent in diabetic patients to manage diabetes and decrease the complications.

scholarly journals Antidiabetic Activity ofVinca roseaExtracts in Alloxan-Induced Diabetic Rats

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Mohammed Fazil Ahmed ◽  
Syed Mohammed Kazim ◽  
Syed Safiullah Ghori ◽  
Syeda Sughra Mehjabeen ◽  
Shaik Rasheed Ahmed ◽  
...  
Keyword(s):  
Body Weight ◽  
Plant Extract ◽  
Low Dose ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
High Dose ◽  
Vinca Rosea ◽  
Methanolic Extracts ◽  
Whole Plant ◽  
Histopathological Studies

The present study was carried out to evaluate the antidiabetic activity ofVinca roseamethanolic whole plant extracts in alloxan induced diabetic rats for 14 days. The methanolic whole plant extract at high dose (500 mg/kg) exhibited significant anti-hyperglycemic activity than whole plant extract at low dose (300 mg/kg) in diabetic rats. The methanolic extracts also showed improvement in parameters like body weight and lipid profile as well as regeneration ofβ-cells of pancreas in diabetic rats. Histopathological studies reinforce the healing of pancreas, by methanolicVinca roseaextracts, as a possible mechanism of their antidiabetic activity.

Antidiabetic activity of Tephrosia tinctoria in Alloxan induced Diabetic Rats: A Preliminary Study

2021 ◽  
pp. 3727-3732
Author(s):  
Vimal John Samuel ◽  
Rashmi DV ◽  
Agasa Ramu Mahesh
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Vital Role ◽  
Antidiabetic Activity ◽  
Protective Effects ◽  
Standard Drug ◽  
Anti Oxidant ◽  
Histopathological Studies

Plant derived products play a vital role in preventing and treating various disease in humans. Tephrosia tinctoria is a plant belonging to the family Leguminosae, found to have antidiabetic and antioxidant activities. The study was aimed to investigate the anti-diabetic and anti-oxidant activity of whole plant of Tephrosia tinctoria in diabetic rats. Alloxan induced model was used to induce Diabetes. The chloroform and ethanolic extracts of Tephrosia tinctoria (CETT and EETT) at the dose of 250 and 500mg/kg b.w were administered orally at single dose per day to diabetic rats. Glipizide 5mg/kg b.w was used as standard drug. The general body weight, insulin level, blood glucose, serum lipid profile, superoxide dismutase, and lipid peroxidation assays were the parameters evaluated in diabetic rats. EETT have better anti-diabetic and anti-oxidant activity than CETT. The protective effects were even confirmed by histopathological studies. These observations show that both the extracts were effective in possessing the significant antidiabetic and antioxidant properties in alloxan induced diabetes.

scholarly journals Evaluation of antidiabetic activity of Tinospora cardifolia in alloxan induced diabetes in albino wistar rats

2018 ◽  
Vol 7 (7) ◽  
pp. 1382 ◽  
Author(s):  
Abhijith L. M. ◽  
Ravi K. Sori
Keyword(s):  
Therapeutic Potential ◽  
Test Group ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
High Dose ◽  
Standard Drug ◽  
Group I

Background: The objective was to evaluate the antidiabetic activity of Tinospora cardifolia in alloxan induced diabetes in albino rats in comparison with a currently used oral hypoglycaemic glibenclamide.Methods: there were 24 rats with FBS in the range 80-115 mg/dl were selected for the study. Four groups each containing six rats, were induced diabetes with alloxan (150mg/kg). The diabetic control group (0.5ml normal saline), Standard control group (5mg/kg glibenclimide), Test group I (200mg/kg T. cardifolia) and test II group 400mg/kg T. cardifolia). FBS was recorded on 1, 3, 7, 14, 21 and 28th day using glucometer. Data was analysed by using one way ANOVA and posthoc Tukey’s test SPSS 21Version.Results: Extract of Tinospora cardifolia showed dependent hypoglycaemic action in both low dose (200mg/kg) and high dose group (400mg/kg). Hypoglycaemic action with high dose of Tinospora cardifolia is comparable to that of standard drug glibenclamide.Conclusions: This study demonstrates the hypoglycaemic action of T. cardifolia in diabetic rats. T. cardifolia can be a therapeutic potential to treat type 2 diabetes mellitus.

scholarly journals Effect of administration of Ehretia anacua aqueous extract on blood glucose level in alloxan - induced diabetic rat

2021 ◽  
Vol 11 (2) ◽  
pp. 001-009
Author(s):  
Oyelade Waheed Abimbola ◽  
Oyebode Joseph Ademola ◽  
Fajilade Temilade Olawande
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Aqueous Extract ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Control Group ◽  
Albino Rats ◽  
Group I ◽  
Histopathological Studies

The effects of crude aqueous extract of Ehretia anacua on alloxan induced diabetic rats was investigated. Male albino rats of weighing between 120 to 150 were used, divided into 6 groups of five animals per group. Group I received distilled water throughout of the experiment and served as the control. Group II received 110 mg/kg of alloxan interperitoneally. Groups III, IV, V and VI received 110 mg/kg of alloxan and in addition administered with aqueous Ehretia anacua extract daily for 14 days. Blood glucose level was monitored at five days interval for fourteen days. Target organs (pancrease) was taken from each rat. The histopathological studies of the pancrease were examined. In alloxan - induced diabetic rats, blood glucose level was significantly increased compared with the control rats. Treating diabetic rats with 50, 100 and 200 mg/kg bw Ehretia anacua caused a significant decrease in the blood glucose level. The Photomicrograph of the histopathology examination of the pancrease (× 100) of the groups treated with alloxan showed poor architecture was destroyed whereas those treated with Ehretia nancua showed normal architecture. This illustrates the amelliorative effects of the extract on the alloxan-induced tocicity. It could be concluded from these results that, Ehretia nancua extract should be used in manufacture processes of the natural products as functional foods or as a dietary supplement with anti-diabecretic activity as hypoglycemic effect.

scholarly journals Anti-diabetic and nephrotoxicity effect of Aegle marmelos leaf on alloxan-induced diabetic rat

2020 ◽  
Vol 11 (3) ◽  
pp. 3966-3971
Author(s):  
Mohd. Sufiyan Siddiqui ◽  
Gaurav Sharma ◽  
Asha Sharma
Keyword(s):  
Uric Acid ◽  
Kidney Function ◽  
Leaf Extract ◽  
Ethanolic Extract ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Aegle Marmelos ◽  
Histopathological Studies ◽  
Ethanolic Leaf Extract

Aegle marmelos is widely found in india. The leaves, roots, fruits, bark, and seeds are extensively used in Ayurveda. . A. marmelos is mainly used for the treatment of diabetes mellitus. Alloxan (150 mg/kg b.w) was used in the Wistar rats for making the diabetic model. The oral administration of leaf extract of Aegle marmelos ( 200 and 400 mg/ kg b.w) was given for four weeks. The effect of ethanolic leaf extract of Aegle marmelos leaf extract on serum blood glucose as well as kidney function test [urea, uric acid, albumin, protein, and creatinine] were measured in the alloxan-induced diabetic rats. In the acute toxicity study, the ethanolic leaf extract of Aegle marmelos leaf was non-toxic at 2000 mg/kg in rats. The level of albumin and protein had significantly increased along with the serum glucose and urea, uric acid levels when they were observed and reduced in diabetic rats treated with both doses of ethanol leaf extract of Aegle marmelos as compared to diabetic group. Histopathological studies were revealed toward normal. Ethanolic extract of Aegle marmelos leaf possesses the significant anti-diabetic and rejuvenating capability of kidney function tests.

scholarly journals Histopathological Profiles of Rats (Rattus norvegicus) Induced with Streptozotocin and Treated with Aqueous Root Extracts of Ruellia tuberosa L.

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Anna Safitri ◽  
Dewi Ratih Tirto Sari ◽  
Bigram Refsilangi ◽  
Anna Roosdiana ◽  
Fatchiyah Fatchiyah
Keyword(s):  
Aqueous Extract ◽  
Rattus Norvegicus ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Standard Drug ◽  
Root Extracts ◽  
Male Wistar Rats ◽  
Diabetic Rat Model ◽  
Antidiabetic Effects

Diabetes mellitus (DM) is a serious worldwide health threat since the number of people with DM is forecasted to grow annually. Thus, effective and affordable treatment is urgently needed. Our previous studies used n-hexane and hydroethanolic root extracts of Ruellia tuberosa L. which significantly affected diabetic rats. In this study, aqueous R. tuberosa L. root extracts were used as treatments for the diabetic rat model and their effects were evaluated. Diabetes was generated by the administration of streptozotocin (STZ) at 20 mg/kg within 5 sequential days. Male Wistar rats were orally treated with the extracts and standard drug (metformin 200 mg/kg) and vehicle every day for 4 weeks. Hypoglycemic effects were assessed for normal, diabetic control, standard, and extract-treated groups. Histopathology was also carried out for the pancreatic, hepatic, and kidney tissues. The progression of diabetes was considerably diminished after extract treatment. In treated rats, the highest dose of extract induced a decline in blood glucose and serum malondialdehyde (MDA) levels at 25% and 35%, respectively. Furthermore, aqueous extract of R. tuberosa L. treatment decreased MDA levels in the pancreas by 12%. Histologic examination of the organ tissues of diabetic rats showed deteriorating alterations. After treatment, histopathological damages to the tissues and cells were reversed. The results of the experiments recommend that aqueous extract of R. tuberosa L. has antidiabetic effects on STZ-induced diabetic rats; nevertheless, a higher dose of the aqueous extracts is needed to achieve more significant results.

scholarly journals AMELIORATIVE EFFECT OF CYPERUS SQUARROSUS (CYPERACEAE) PLANT EXTRACTS ON DIABETIC CARDIOMYOPATHY AGAINST STREPTOZOTOCININDUCED DIABETES IN WISTAR RATS

2020 ◽  
pp. 160-165
Author(s):  
RAGHAVENDRA HG ◽  
RAVI PRAKASH P ◽  
DEVANNA N
Keyword(s):  
Aqueous Extract ◽  
Density Lipoprotein ◽  
Ethanolic Extract ◽  
Treated Group ◽  
Cardiovascular Complications ◽  
Serum Glucose ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Standard Drug ◽  
Ameliorative Effect

Objective: Cyperus squarrosus, belongs to the family Cyperaceae, has been claimed to possess antidiabetic activity in the ethnomedicinal literature in India. Therefore, the present study was to investigate the effects of aqueous and ethanolic extracts of C. squarrosus (EECS) on diabetes and its cardiovascular complications with streptozotocin-induced diabetes in rats. Methods: Wister albino rats of either sex were made diabetic with streptozotocin (65 mg/kg, i.v.). Glibenclamide (5 mg/kg) was taken as standard drug. Treatment of aqueous and EECS (aqueous extract of C. squarrosus and EECS) was given in the dose of 200 and 400 mg/kg/day, p.o for 8 weeks and biochemical (serum glucose, cholesterol, triglycerides, and high-density lipoprotein [HDL]) parameters were recorded. Results: Streptozotocin-treated group produced significant increased levels of serum glucose, cholesterol, triglycerides, HDL, creatine kinase-myocardial band (CK-MB), and lactate dehydrogenase (LDH) levels and all these changes were prevented by the treatment with aqueous and EECS in both doses. Conclusion: As compare with ethanolic extract treated group, aqueous extract treated group exhibits significant (p<0.001) effect on CK-MB and LDH levels. Our result suggests that aqueous and EECS prevents the streptozotocin-induced metabolic abnormalities as well as cardiovascular complications.

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