scholarly journals Herba Epimedii extraction overcome Rosiglitazone induced bone loss in diabetic rats

2021 ◽  
Vol 8 (9) ◽  
pp. 7-14
Author(s):  
Khalid A Asseri ◽  
◽  
Yahya I Asiri ◽  
Ali Alqahtani ◽  
Krishnaraju Venkatesan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bone Loss ◽  
Bone Fractures ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Osteoporosis Therapy ◽  
Diabetes Group ◽  
Herba Epimedii ◽  
Turnover Markers ◽  
The Impact

Diabetes mellitus patients are more prone to suffer from bone fractures. Herba Epimedii has been demonstrated to be an osteoporosis reducer in the past. However, it is yet uncertain if Herba Epimedii may protect diabetic rats from bone loss when co-administered with rosiglitazone (RSG). This study examines the impact of Herba Epimedii on bone oxidative stress and turnover markers in diabetic rats co-treated with rosiglitazone (RSG). Streptozotocin (STZ) causes diabetics. Wistar albino rats were placed into five groups, each with six rats: control (vehicle therapy), Streptozotocin (diabetes) group, Herba Epimedii group, Rosiglitazone, and Rosiglitazone +Herba Epimedii. Each medication was given by gastric gavage once a day for 35 days. Insulin, oxidative stress, and bone turnover markers were measured in the blood using ELISA assays. Insulin and osteocalcin levels were significantly higher in diabetic rats administered Herba Epimedii than in diabetic control rats. Herba Epimedii may be able to prevent diabetic osteoporosis in RSG-treated diabetic rats by enhancing osteogenesis and lowering bone oxidative stress. The utility of Herba Epimedii as an osteoporosis therapy in diabetic individuals is supported by these findings. Keywords: Herba Epimedii, Diabetes, Osteoporosis, Rosiglitazone

Radix Dipsaci extract protects against Rosiglitazone induced bone loss

2021 ◽  
Vol 8 (9) ◽  
pp. 15-21
Author(s):  
Khalid A Asseri ◽  
◽  
Yahya I Asiri ◽  
Ali Alqahtani ◽  
Krishnaraju Venkatesan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bone Loss ◽  
Bone Fractures ◽  
Scientific Evidence ◽  
Streptozotocin Diabetes ◽  
Diabetic Control ◽  
Albino Rats ◽  
Protective Effects ◽  
Diabetes Group ◽  
Diabetic Osteoporosis

The dried root of Dipsacus asperoides is known as Radix Dipsaci extract(RDE). It's a kidney-toning herbal medication with a lengthy track record of safe usage in the treatment of bone fractures and joint disorders. The drug rosiglitazone (RSG) causes an imbalance in bone remodelling, which results in increased apoptotic death of osteogenic cells and decreased bone production. The goal of this study was to investigate the effects of RDE on RSGinduced bone loss in diabetic rats in a systematic way. Five groups of six Wistar albino rats were studied: control (vehicle therapy), Streptozotocin (diabetes) group, RDE group, Rosiglitazone, and Rosiglitazone +RDE group. Insulin, oxidative stress, and bone turnover markers in the blood were all detected using ELISA tests. When compared to diabetic control rats, RDE therapy significantly raised insulin and osteocalcin levels. RDE may be able to prevent diabetic osteoporosis by boosting osteogenesis and lowering oxidative stress in the bone.These findings support the use of RDE as a bone loss inhibiting in diabetics. Well-designed clinical trials are likely to yield further scientific evidence on its bone-protective effects and safety. Keywords: Radix Dipsaci, Diabetic osteoporosis, Rosiglitazone.

scholarly journals The Antioxidant Effect of Curcumin and Rutin on Oxidative Stress Biomarkers in Experimentally Induced Periodontitis in Hyperglycemic Wistar Rats

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1332
Author(s):  
Gilda M. Iova ◽  
Horia Calniceanu ◽  
Adelina Popa ◽  
Camelia A. Szuhanek ◽  
Olivia Marcu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Rats ◽  
Gingival Tissue ◽  
Control Group ◽  
Albino Rats ◽  
Oxidative Stress Biomarkers ◽  
Significant Difference ◽  
The Impact ◽  
Experimentally Induced

Background: There is a growing interest in the correlation between antioxidants and periodontal disease. In this study, we aimed to investigate the effect of oxidative stress and the impact of two antioxidants, curcumin and rutin, respectively, in the etiopathology of experimentally induced periodontitis in diabetic rats. Methods: Fifty Wistar albino rats were randomly divided into five groups and were induced with diabetes mellitus and periodontitis: (1) (CONTROL)—control group, (2) (DPP)—experimentally induced diabetes mellitus and periodontitis, (3) (DPC)—experimentally induced diabetes mellitus and periodontitis treated with curcumin (C), (4) (DPR)—experimentally induced diabetes mellitus and periodontitis treated with rutin (R) and (5) (DPCR)—experimentally induced diabetes mellitus and periodontitis treated with C and R. We evaluated malondialdehyde (MDA) as a biomarker of oxidative stress and reduced glutathione (GSH), oxidized glutathione (GSSG), GSH/GSSG and catalase (CAT) as biomarkers of the antioxidant capacity in blood harvested from the animals we tested. The MDA levels and CAT activities were also evaluated in the gingival tissue. Results: The control group effect was statistically significantly different from any other groups, regardless of whether or not the treatment was applied. There was also a significant difference between the untreated group and the three treatment groups for variables MDA, GSH, GSSG, GSH/GSSG and CAT. There was no significant difference in the mean effect for the MDA, GSH, GSSG, GSH/GSSG and CAT variables in the treated groups of rats with curcumin, rutin and the combination of curcumin and rutin. Conclusions: The oral administration of curcumin and rutin, single or combined, could reduce the oxidative stress and enhance the antioxidant status in hyperglycemic periodontitis rats.

The Impact of L-Carnitine and Coenzyme Q10 as Protection Against Busulfan-Oxidative Stress in the Liver of Adult Rats

2020 ◽  
Vol 10 (5) ◽  
pp. 578-586
Author(s):  
Areeg M. Abdelrazek ◽  
Shimaa A. Haredy
Keyword(s):  
Oxidative Stress ◽  
Coenzyme Q10 ◽  
Protective Role ◽  
Albino Rats ◽  
Distilled Water ◽  
Negative Effects ◽  
Adult Rats ◽  
Stress Damage ◽  
The Impact ◽  
Liver Tissues

Background: Busulfan (Bu) is an anticancer drug with a variety of adverse effects for cancer patients. Oxidative stress has been considered as a common pathological mechanism and it has a key role in the initiation and progression of liver injury by Bu. Aim: The study aimed to evaluate the antioxidant impact of L-Carnitine and Coenzyme Q10 and their protective role against oxidative stress damage in liver tissues. Methods and Material: Thirty-six albino rats were divided equally into six groups. G1 (con), received I.P. injection of DMSO plus 1 ml of distilled water daily by oral gavages; G2 (Bu), received I.P. injection of Bu plus 1 ml of the distilled water daily; G3 (L-Car), received 1 ml of L-Car orally; G4 (Bu + L-Car) received I.P. injection of Bu plus 1 ml of L-Car, G5 (CoQ10) 1 ml of CoQ10 daily; and G6 (Bu + CoQ10) received I.P. injection of Bu plus 1 ml of CoQ10 daily. Results: The recent data showed that Bu induced significant (P<0.05) elevation in serum ALT, AST, liver GSSG, NO, MDA and 8-OHDG, while showing significant (P<0.05) decrease in liver GSH and ATP. On the other hand, L-Carnitine and Coenzyme Q10 ameliorated the negative effects prompted by Bu. Immunohistochemical expression of caspase-3 in liver tissues reported pathological alterations in Bu group while also showed significant recovery in L-Car more than CoQ10. Conclusion: L-Car, as well as CoQ10, can enhance the hepatotoxic effects of Bu by promoting energy production in oxidative phosphorylation process and by scavenging the free radicals.

Attenuation of erythrocyte membrane oxidative stress bySesbania grandiflorain streptozotocin-induced diabetic rats

2015 ◽  
Vol 93 (4) ◽  
pp. 385-395 ◽  
Author(s):  
Chandrabose Sureka ◽  
Thiyagarajan Ramesh ◽  
Vavamohaideen Hazeena Begum
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Blood Glucose ◽  
Erythrocyte Membrane ◽  
Osmotic Fragility ◽  
Diabetic Rats ◽  
Glycosylated Haemoglobin ◽  
Albino Rats ◽  
Enzymatic Antioxidants ◽  
Protective Effects

The aim of the present study was to investigate the protective effects of Sesbania grandiflora flower (SGF) extract on erythrocyte membrane in Streptozotocin (STZ)-induced diabetic rats. Adult male albino rats of Wistar strain, weighing 190–220 g, were made diabetic by an intraperitonial administration of STZ (45 mg/kg). Normal and diabetic rats were treated with SGF, and diabetic rats were also treated with glibenclamide as drug control, for 45 days. In this study plasma insulin and haemoglobin levels were decreased and blood glucose, glycosylated haemoglobin, protein oxidation, lipid peroxidation markers, and osmotic fragility levels were increased in diabetic rats. Moreover, erythrocytes antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxide, glutathione reductase, glutathione-S-transferase, and glucose-6-phosphate dehydrogenase activities and non-enzymatic antioxidants such as vitamin C, vitamin E, reduced glutathione (GSH), and oxidized glutathione (GSSG) levels were altered. Similarly, the activities of total ATPases, Na+/K+-ATPase, Ca2+-ATPase, and Mg2+-ATPase were also decreased in the erythrocytes of diabetic rats. Administration of SGF to STZ-induced diabetic rats reduced blood glucose and glycosylated haemoglobin levels with increased levels of insulin and haemoglobin. Moreover, SGF reversed the protein and lipid peroxidation markers, osmotic fragility, membrane-bound ATPases activities, and antioxidant status in STZ-induced diabetic rats. These results suggest that SGF could provide a protective effect on diabetes by decreasing oxidative stress-associated diabetic complications.

HIGH GLUCOSE INDUCED BONE LOSS VIA ATTENUATING THE PROLIFERATION AND OSTEOBLASTOGENESIS AND ENHANCING ADIPOGENESIS OF BONE MARROW MESENCHYMAL STEM CELLS

2013 ◽  
Vol 25 (05) ◽  
pp. 1340010 ◽  
Author(s):  
Wen-Tyng Li ◽  
Wen-Kai Hu ◽  
Feng-Ming Ho
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bone Loss ◽  
High Glucose ◽  
Chromatin Condensation ◽  
Diabetic Rats ◽  
Peroxisome Proliferator ◽  
Bone Marrow Mscs

Diabetes mellitus (DM) is associated with bone loss and leads to osteopenia and osteoporosis. This study was undertaken to investigate whether the impaired functions of mesenchymal stem cells (MSCs) derived from bone marrow play a role in pathogenesis of DM-associated bone loss. Bone marrow MSCs were taken from the alloxan-induced diabetic rats and normal rats. Bone mineral densities of tibias and femurs in diabetic rats decreased compared to those of normal rats as shown by dual energy X-ray absorptiometry. MSCs from diabetic rats exhibited reduced colony formation activity. The in vitro effects of high glucose (HG) (20 or 33 mM) on the growth, oxidative stress, apoptosis, and differentiation MSCs were next assessed. The viability and proliferation of MSCs derived from diabetic rats decreased significantly compared with that from normal rats. HG further suppressed the proliferation and viability of MSCs from both diabetic and normal rats. HG was associated with 38–40% increase in reactive oxygen species level and had significantly downregulated the activities of superoxide dismutase (SOD) and catalase (CAT) which could be recovered by the addition of L-ascorbic acid. The phenomena of apoptosis such as chromatin condensation and DNA fragmentation were found in cells cultured under HG conditions. As compared with 5.5 mM glucose, exposure of MSCs to HG enhanced adipogenic induction of triacylglycerol accumulation and inhibited osteogenic induction of alkaline phosphatase activity. HG increased peroxisome proliferator-activated receptor gamma expression during adipogenesis and reduced RUNX2 expression during osteoblastogenesis. These results indicate that MSCs derived from diabetic rats exhibited the inhibitory effects on cell growth and osteogenic ability. The oxidative stress, apoptosis, and adipogenic capability of MSCs were increased by HG. Furthermore, it is suggested that HG induces bone loss via attenuating the proliferation and osteoblastogenesis and enhancing adipogenesis mediated by the oxidative stress in rat bone marrow MSCs.

scholarly journals Oat Attenuation of Hyperglycemia-Induced Retinal Oxidative Stress and NF-B Activation in Streptozotocin-Induced Diabetic Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Abdulrahman L. Al-Malki
Keyword(s):  
Oxidative Stress ◽  
Diabetic Retinopathy ◽  
Protective Role ◽  
Endothelial Damage ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Mobility Shift ◽  
Microvascular Damage ◽  
Necrosis Factor Alpha ◽  
Diabetic Retina

The overproduction of reactive oxygen species (ROS) plays a central role in the pathogenesis of endothelial damage in diabetes. To assess the effect of oat on experimental diabetic retinopathy, five groups of Albino rats were studied: nondiabetic control, untreated diabetic, and diabetic rats treated with 5%, 10%, and 20% (W/W) oat of the diet for 12 weeks. Novel data were obtained in this study indicating a protective role of oat against oxidative stress and diabetic retinopathy. The effects of oat on parameters of oxidative stress, AGE, and nuclear factor kappa B (NF-B) were assessed by ELISA and NF-B activation by electrophoretic mobility shift assay. Tumor necrosis factor alpha (TNF) and vascular endothelial growth factor (VEGF) were also determined. After 12 weeks of diabetes, oat treatment reduced blood glucose levels, HbA1c, all oxidative stress markers, CML, normalized NF-B activation and TNF expression. Furthermore it reduced VEGF in the diabetic retina by 43% (). In conclusion, oat modulates microvascular damage through normalized pathways downstream of ROS overproduction and reduction of NF-B and its controlled genes activation, which may provide additional endothelial protection.

scholarly journals Protective effect of crocin and voluntary exercise against oxidative stress in the heart of high-fat diet-induced type 2 diabetic rats

2016 ◽  
Vol 103 (4) ◽  
pp. 459-468 ◽  
Author(s):  
V Ghorbanzadeh ◽  
M Mohammadi ◽  
G Mohaddes ◽  
H Dariushnejad ◽  
L Chodari ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
High Fat Diet ◽  
Critical Role ◽  
Diabetic Rats ◽  
Voluntary Exercise ◽  
High Fat ◽  
The Impact ◽  
Type 2 Diabetic

Background Oxidative stress plays a critical role in the pathogenesis and progression of type 2 diabetes and diabetic-associated cardiovascular complications. This study investigated the impact of crocin combined with voluntary exercise on heart oxidative stress indicator in high-fat diet-induced type 2 diabetic rats. Materials and methods Rats were divided into four groups: diabetes, diabetic-crocin, diabetic-voluntary exercise, diabetic-crocin-voluntary exercise. Type 2 diabetes was induced by high-fat diet (4 weeks) and injection of streptozotocin (intraperitoneally, 35 mg/kg). Animals received crocin orally (50 mg/kg); voluntary exercise was performed alone or combined with crocin treatment for 8 weeks. Finally, malondialdehyde (MDA), activity of antioxidant enzymes, superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) were measured spectrophotometrically. Results Treatment of diabetic rats with crocin and exercise significantly decreased the levels of MDA (p < 0.001) and increased the activity of SOD, GPx, and CAT compared with the untreated diabetic group. In addition, combination of exercise and crocin amplified their effect on antioxidant levels in the heart tissue of type 2 diabetic rats. Conclusion We suggest that a combination of crocin with voluntary exercise treatment may cause more beneficial effects in antioxidant defense system of heart tissues than the use of crocin or voluntary exercise alone.

scholarly journals Antioxidant Potentials of Aqueous and Ethanolic Extracts of Morus mesozygia Linn Stapf Twigs in Streptozotocin-Induced Diabetic Rats

2021 ◽  
pp. 36-46
Author(s):  
Marcella Tari Joshua ◽  
Edna O. Wachuku ◽  
N. Boisa ◽  
Nsirim Nduka
Keyword(s):  
Oxidative Stress ◽  
Diabetic Rats ◽  
Stress Index ◽  
Albino Rats ◽  
Total Oxidant Status ◽  
Oxidative Stress Index ◽  
Methanolic Extracts ◽  
Total Antioxidant ◽  
Oxidant Status ◽  
Antioxidant Effects

Aim: The aim of this study was to assess the antioxidant effects of aqueous, ethanolic and methanolic extracts of Morus mesozygia Linn. Stapf. Twigs in Streptozotocin-Induced Diabetic Rats. Study Design: The study is an experimental case-controlled study. Place and Duration of Study: This study was carried out at the Biochemistry Research Laboratory, University of Port Harcourt, Rivers State, Nigeria, between June, 2018 and April, 2019. Methodology: A total of 65 male albino rats that weighed between 150g to 200g were used for this research study. Three different extracted solvents; aqueous, ethanolic and methanolic twig extracts were administered to different groups of the rats. The male albino rats for this study were induced with a single dose of 40mg/kg b.wt, intraperitoneally of streptozotocin in 0.1M of citrate buffer, pH 4.5. The diabetic male rats were those whose fasting blood glucose (FBG) were from 250mg/dl or 13mmol/L and above. They were then divided into different groups and treated with different concentrations of aqueous, ethanolic and methanolic extracts of the plant material. At the end of treatment period, the rats were kept on fasting for 6 hours prior to the process of euthanasia, they were sacrificed and blood samples were collected through cardiac puncture for analysis into lithium heparin bottle for the estimation of oxidative stress markers, malondaldehyde (MDA), total oxidant status (TOS), oxidative stress index (OSI), and antioxidant enzymes, superoxide dismutase (SOD), total antioxidant capacity (TAS). Statistical analysis was done using GraphPad prism (version 6.1) software. Data generated were represented as mean and standard deviations (Mean ±S. D). Level of significant at Tukey’s Multiple Comparative Test was tested at p<0.0001. Charts were made possible with the application of Minitab version 2019. Results: The results showed that there were significant increases in the levels of superoxide dismutase (SOD, 414.2±1.30) ng/ml, total antioxidant status (TAS, 82.97±7.71) mU/ml, total oxidant status (TOS, 355.02± 14.02) mU/ml activity, a reduced oxidative stress index of 4.29±0.26 and concentration of malondialdehyde (MDA of 18.67± 0.26mmol/L) when rats were treated with 400mg/kg of aqueous leaves of Morus mesozygia Linn. S. When compared with those of rats treated with 200mg/kg of aqueous leaf extracts of MMLS. there was a significant increases and decreases respectively. Other methods of extractions (methanolic and ethanolic), also improved the antioxidant statuses of the diabetes induced and treated rats after treatment of the extracts. Conclusion: The three extracts of Morus mesozygia Linn. S showed tremendous antioxidant effects against Streptozotocin-induced diabetic rats, with the methanolic extract showing the most potent effect.

scholarly journals The Effects of Grape Seed Extract on Insulin, Adiponectin and Resistin Levels in Diabetic Rats

2021 ◽  
Vol 9 (4) ◽  
pp. 709-713
Author(s):  
İnci Söğütlü ◽  
Nihat Mert ◽  
Handan Mert ◽  
Leyla Mis ◽  
Halil Cumhur Yılmaz ◽  
...  
Keyword(s):  
Insulin Level ◽  
Grape Seed Extract ◽  
Grape Seed ◽  
Diabetic Rats ◽  
Seed Extract ◽  
Control Group ◽  
Albino Rats ◽  
Diabetes Group ◽  
Positive Effects ◽  
Wistar Albino Rats

In this study, the effects of grape seed extract on insulin, adiponectin and resistin levels in diabetic rats were aimed to investigate. Weight of about 300-350g 7-8 weeks 32 female Wistar Albino rats were used. Weight of about 300-350g 7-8 weeks 32 female Wistar Albino rats randomly divided into four groups of eight each: control group (C), group with diabetes (DM), grape seed extract group (GSE), diabetes mellitus and grape seed extract group (DM+GSE). The diabetic group 45 mg/kg single dose of streptozotocin was administered intraperitoneally, and the grape seed extract groups (DM+GSE and GSE) grape seed extract was given orally every day with an intragastric tube for 20 days (0.6 ml/rat). Blood samples were taken from the hearts of rat’s end of the experiment. The sera obtained were used for insulin, adiponectin and resistin analysis measured via ELISA with commercial kits. The insulin and adiponectin levels of the rats in the diabetes + grape seed extract group were found to be higher than those in the diabetes group. There was no significant change in the resistin level differences between the groups. As a result; It is possible to say that grape seed extract has an increasing effect on insulin level and adiponectin levels of diabetic rats are decreased due to diabetes and it has no significant effect on resistin levels. Detected by the positive effects of grape seed extracts, it may be useful to use for the regulating of the diabetes in human.

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