scholarly journals Evaluation of Antidiabetic and Antihyperlipidemic Effect of Ethanolic Leaves Extract of Centratherum anthelminticum (L) Kuntze against STZ Induced Diabetic Rats

2020 ◽  
Vol 10 (2-s) ◽  
pp. 140-148
Author(s):  
Nidhi Goutam ◽  
Reni Kapoor
Keyword(s):  
Glycogen Phosphorylase ◽  
Chronic Administration ◽  
Inhibitory Effect ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Keywords Diabetes ◽  
Antihyperlipidemic Activity ◽  
Key Enzyme ◽  
Centratherum Anthelminticum ◽  
Altered Lipid

Objective: To evaluate the Antidiabetic & Antihyperlipidemic effect of Centratherum Anthelminticum (L) Kuntze extract against STZ induced diabetic rats. Methods: The present study examined the influence of Centratherum Anthelminticum extract (CASE), on blood glucose level and antioxidant enzymes level in STZ induced diabetic rats. The possible effects of chronic administration of the extract on the fasting serum glucose, insulin, and total cholesterol and triglycerides levels in STZ-induced diabetic rats were also evaluated. Results: The extracts showed a marked Antidiabetic & Antihyperlipidemic effect. Centratherum anthelminticum ethanolic seeds extract is capable of exhibiting glycemic control through its inhibitory effect on glycogenolytic enzyme glycogen phosphorylase, gluconeogenic enzymes such as glucose-6-phosphatase and fructose-1, 6-bis phosphatase and stimulatory effect on hexokinase, which is the key enzyme in the glycolytic pathway. Antihyperlipidemic activity of Centratherum anthelminticum ethanolic seeds extract proved by restoration of altered lipid profile Conclusions: The results of the study indicate that the extract of Centratherum anthelminticum possesses strong antidiabetic & antihyperlipidemic activity. This study also describes therapeutic effect of Centratherum anthelminticum in diabetes which will give a new direction for the future scientific research. Keywords: Diabetes, Centratherum anthelminticum, Pharmacological, Antidiabetic, Antihyperlipidemic

scholarly journals An In Silico and an In Vitro Inhibition Analysis of Glycogen Phosphorylase by Flavonoids, Styrylchromones, and Pyrazoles

Nutrients ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 306
Author(s):  
Sónia Rocha ◽  
Natália Aniceto ◽  
Rita C. Guedes ◽  
Hélio M. T. Albuquerque ◽  
Vera L. M. Silva ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
In Silico ◽  
Glycogen Phosphorylase ◽  
Inhibitory Effect ◽  
Docking Studies ◽  
Potent Inhibitory Effect ◽  
Key Enzyme ◽  
Experimental Findings

Glycogen phosphorylase (GP) is a key enzyme in the glycogenolysis pathway. GP inhibitors are currently under investigation as a new liver-targeted approach to managing type 2 diabetes mellitus (DM). The aim of the present study was to evaluate the inhibitory activity of a panel of 52 structurally related chromone derivatives; namely, flavonoids, 2-styrylchromones, 2-styrylchromone-related derivatives [2-(4-arylbuta-1,3-dien-1-yl)chromones], and 4- and 5-styrylpyrazoles against GP, using in silico and in vitro microanalysis screening systems. Several of the tested compounds showed a potent inhibitory effect. The structure–activity relationship study indicated that for 2-styrylchromones and 2-styrylchromone-related derivatives, the hydroxylations at the A and B rings, and in the flavonoid family, as well as the hydroxylation of the A ring, were determinants for the inhibitory activity. To support the in vitro experimental findings, molecular docking studies were performed, revealing clear hydrogen bonding patterns that favored the inhibitory effects of flavonoids, 2-styrylchromones, and 2-styrylchromone-related derivatives. Interestingly, the potency of the most active compounds increased almost four-fold when the concentration of glucose increased, presenting an IC50 < 10 µM. This effect may reduce the risk of hypoglycemia, a commonly reported side effect of antidiabetic agents. This work contributes with important considerations and provides a better understanding of potential scaffolds for the study of novel GP inhibitors.

scholarly journals Pre treatment by the crude and the n-hexane extract of Nigella sativa Linn. (Kalajira) alleviates diabetes mellitus

1970 ◽  
pp. 5-9
Author(s):  
K Matira ◽  
ZF Dewan ◽  
TC Rehnuma ◽  
A Farhana ◽  
UN Zeba ◽  
...  
Keyword(s):  
Nigella Sativa ◽  
Medical Science ◽  
Elevated Serum ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Hexane Extract ◽  
Keywords Diabetes ◽  
Pretreated Group ◽  
Pre Treatment ◽  
Higher Doses

Methodology: Effect of pretreatment by crude and n-Hexane extract of Nigella (10mg/kg body wt./day for 21 days) on streptozotocin (STZ, 50 mg/kg body wt. single dose i.p.) administered rats were observed in the present study. Biochemical parameters (Serum glucose, serum TG, serum cholesterol, pancreatic GSH) were compiled together with pancreatic histology. Results: Both the crude and the n-Hexane extract of Nigella pre-treated diabetic rats had demonstrated significant (P<0.001) alleviation from the elevated serum glucose, lowered pancreatic reduced glutathione (P<0.01) and elevated serum TG concentrations (P<0.01). 72% β cells appeared to be damaged by STZ administration, while in the crude and the n-Hexane extract of Nigella pretreated diabetic groups this damage was 31% and 46% respectively, the crude Nigella pretreated group thus appeared to have better amelioration. Conclusions: Further studies are suggested to obtain the protective ingredient from the crude Nigella and to observe its effect upon the above mentioned parameters of diabetic rats in higher doses for prolonged periods. Keywords: Diabetes, Nigella sativa Linn., Streptozotocin. doi: 10.3329/bjms.v8i1.3182 Bangladesh Journal of Medical Science Vol.8 No. 1-2; 2009 5-9

scholarly journals Antidiabetic Potential of Stem Bark Extract of Enantia chlorantha and Lack of Modulation of Its Therapeutic Efficacy in Diabetic Rats Co-Administered with Lisinopril

2021 ◽  
Vol 68 (1) ◽  
pp. 127-127
Author(s):  
Latifat Bolanle Ibrahim ◽  
Patience Funmilayo Idowu ◽  
Opemipo Adekanye Moses ◽  
Mutiu Adewunmi Alabi ◽  
Emmanuel Oladipo Ajani
Keyword(s):  
Lipid Profile ◽  
Stem Bark ◽  
Inhibitory Effect ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Bark Extract ◽  
Therapeutic Implications ◽  
Glucose Levels

This study validates the antidiabetic efficacy of Enantia chlorantha stem bark and the possible therapeutic implications of the co-administration of lisinopril and E. chlorantha in type 2 diabetic rats. E. chlorantha stem bark was extracted by cold maceration. The inhibitory effect of the plant on carbohydrate metabolizing enzymes and its antioxidative potentials were assessed in vitro. The extract exhibited α-amylase and α-glucosidase inhibitory activities and also showed antioxidative properties in vitro. Administration of the extract normalized fasting hyperglycemia in vivo by showing 47.24% reduction in blood glucose levels relative to untreated diabetic rats. Co-administration of E. chlorantha and lisinopril restored serum glucose and serum lipid profile levels. E. chlorantha stem bark displayed antidiabetic potentials as compared with a standard antidiabetic drug (metformin). The study also showed that the plant contained some bioactive compounds which we hypothesize might be responsible for the observed activities. Co-administration of the plant with lisinopril conferred no significant therapeutic advantage on the serum glucose level and lipid profile.

Evaluation of Anti-diabetic and Anti-hyperlipidemic Activity of Isolated Bioactive Compounds of Leaves of Annona reticulata Linn.

2020 ◽  
Vol 10 ◽  
Author(s):  
Kalyani Pathak ◽  
Aparoop Das ◽  
Anshul Shakya ◽  
Riya Saikia ◽  
Himangshu Sarma
Keyword(s):  
Spectral Analysis ◽  
Gallic Acid ◽  
Liver Enzymes ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Traditional Use ◽  
Per Oral ◽  
Diabetes And Obesity ◽  
Annona Reticulata

Background: The leaves of Annona reticulata Linn. have been traditionally used by the tribes of Assam as a source of medicine to mitigate a range of health ailments including diabetes and obesity. Objectives: The current study aimed to evaluate the anti-diabetic and anti-hyperlipidemic potential of bioactive fractions isolated from the methanolic extract of Annona reticulata Linn. leaves using Nicotinamide + Streptozotocin (60 mg/kg, i.p.) induced diabetic rats. Methods: The partially purified bioactive fractions, namely F1, F2, F3 and F4 were administered to diabetic rats with the dose of 200 mg/kg, per oral (p.o.) and the effect of the fractions on serum glucose were studied up to 21 days. The potent fractions were further subjected for spectral analysis for identification of the isolated active compounds. Results: The in-vivo anti-diabetic activity of the isolated fractions F2 and F3 were found significant controlling blood glucose level, alike glibenclamide. Interestingly, F2 and F3 treated animals were found significant in restoring the lipid and liver enzymes profile in streptozotocin challenge rats. Further, spectral analysis revealed that F2 and F3 were comprises Quercetin and Gallic acid, respectively. Conclusion: Outcome of finding demonstrate the anti-diabetic and anti-hyperlipidemic potential of the isolates/fractions of A. reticulata, which were found enriched in polyphenolics including Quercetin and Gallic acid; and provides logistic behind the traditional use of the A. reticulata against Diabetes and obesity.

Hepato-renal protective effects of gallic acid and p-coumaric acid in nicotinamide/streptozotocin-induced diabetic rats

2016 ◽  
Vol 5 (06) ◽  
pp. 4641 ◽  
Author(s):  
Adel Abdel Moneim* ◽  
Sanaa M. Abd El-Twab ◽  
Mohamed B. Ashour ◽  
Ahmed I. Yousef
Keyword(s):  
Body Weight ◽  
Gallic Acid ◽  
Protein Profile ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Hypoglycemic Agents ◽  
Protective Effects ◽  
Coumaric Acid ◽  
Experimental Type

The goal of diabetes treatment is primarily to save life and alleviate symptoms and secondary to prevent long-term diabetic complications resulting from hyperglycemia. Thus, our present investigation was designed to evaluate the hepato-renal protective effects of gallic acid and p-coumaric acid in nicotinamide/streptozotocin (NA/STZ)-induced diabetic rats. Experimental type 2 diabetes was induced by a single intraperitoneal (i.p.) injection of STZ (65 mg/kg b.wt.), after 15 min of i.p. injection of NA (120 mg/kg b.wt.). Gallic acid and p-coumaric acid were orally administered to diabetic rats at a dose of 20, 40 mg/kg b.wt./day, respectively, for 6 weeks. Body weight, serum glucose, protein profile, liver function enzymes and kidney function indicators was assayed. Treatment with either gallic acid or p-coumaric acid significantly ameliorated the elevated levels of glucose, alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea and uric acid. Both compounds were also found to restore total protein, albumin, and globulin as well as body weight of diabetic rats to near normal values. It can conclude that both gallic acid and p-coumaric acid have potent hypoglycemic and hepato-renal protective effects in diabetic rats. Therefore, our results suggest promising hypoglycemic agents that can attenuate the progression of diabetic hepatopathy and nephropathy.

Effects of cortisol and progesterone on insulin binding and lipogenesis in adipocytes from normal and diabetic rats

1985 ◽  
Vol 106 (2) ◽  
pp. 225-231 ◽  
Author(s):  
A.-M. Mendes ◽  
R. J. Madon ◽  
D. J. Flint
Keyword(s):  
Body Weight ◽  
Adipose Tissue ◽  
Insulin Sensitivity ◽  
Insulin Receptor ◽  
Body Weight Gain ◽  
Serum Insulin ◽  
Insulin Binding ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Receptor Concentration

ABSTRACT Cortisol implants in normal and diabetic rats reduced body weight, adiposity, insulin receptor concentration and both basal and insulin-stimulated rates of lipogenesis in isolated adipocytes, whilst insulin sensitivity was unchanged. In normal but not diabetic rats these changes were accompanied by increased serum glucose and insulin concentrations. In contrast, progesterone implants in normal and diabetic rats increased body weight gain, adiposity, insulin receptor concentration and both basal and insulin-stimulated rates of lipogenesis in adipose tissue, again without affecting insulin sensitivity. Progesterone did not affect serum insulin concentrations in normal or diabetic rats but accelerated the decline in serum glucose concentrations which occurred during an overnight fast in diabetic rats. The results suggest that (1) cortisol inhibits lipogenesis in adipose tissue without affecting insulin sensitivity, (2) cortisol reduces insulin binding in adipose tissue without a requirement for hyperinsulinaemia, which might itself indirectly lead to down-regulation of the insulin receptor, and (3) in diabetic rats progesterone stimulates lipogenesis in adipose tissue without any increase in food intake or serum insulin concentrations suggesting that progesterone may have a direct anabolic role in adipose tissue. J. Endocr. (1985) 106, 225–231

scholarly journals Characterization of hyperglycemia due to sub-chronic administration of red ginseng extract via comparative global proteomic analysis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ann-Yae Na ◽  
Jung Jae Jo ◽  
Oh Kwang Kwon ◽  
Piljoung Cho ◽  
Yan Gao ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Herbal Remedy ◽  
Chronic Administration ◽  
Serum Glucose ◽  
Control Group ◽  
Red Ginseng ◽  
Korean Red Ginseng ◽  
Ginseng Extract ◽  
Glucose Levels ◽  
Term Administration

AbstractGinseng (Panax ginseng Meyer) is commonly used as an herbal remedy worldwide. Few studies have explored the possible physiological changes in the liver although patients often self-medicate with ginseng preparations, which may lead to exceeding the recommended dose for long-term administration. Here, we analyzed changes in the hepatic proteins of mouse livers using quantitative proteomics after sub-chronic administration of Korean red ginseng (KRG) extract (control group and 0.5, 1.0, and 2.0 g/kg KRG) using tandem mass tag (TMT) 6‐plex technology. The 1.0 and 2.0 g/kg KRG groups exhibited signs of liver injury, including increased levels of aspartate transaminase (AST) and alanine aminotransferase (ALT) in the serum. Furthermore, serum glucose levels were significantly higher following KRG administration compared with the control group. Based on the upregulated proteins found in the proteomic analysis, we found that increased cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CSE) levels promoted greater hydrogen sulfide (H2S) synthesis in the liver. This investigation provides novel evidence that sub-chronic administration of KRG can elevate H2S production by increasing protein expression of CBS and CSE in the liver.

scholarly journals ARISTOLOCHIA BRACTEOLATA – ANTIHYPERGLYCEMIC AND ANTIHYPERLIPIDEMIC ACTIVITY IN DEXAMETHASONE-INDUCED DIABETIC RAT MODEL

2017 ◽  
Vol 10 (8) ◽  
pp. 75
Author(s):  
Ganga Rajum ◽  
Hema Sundar Reddy T ◽  
Hema Sundar Reddy T
Keyword(s):  
Blood Glucose ◽  
Methanolic Extract ◽  
Density Lipoprotein ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Standard Drug ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Antihyperlipidemic Activity ◽  
Aristolochia Bracteolata

  Objective: The present study was aimed to evaluate antihyperglycemic and antihyperlipidemic activities of methanolic extract of Aristolochia bracteolata (MEAB) against dexamethasone-induced diabetic rat model.Methods: Methanolic extract was prepared by soxhlet extraction and was evaluated for antihyperglycemic and antihyperlipidemic activity using dexamethasone-induced model. The MEAB was administered orally at a dose of 200 and 400 mg/kg body weight glibenclamide was used as standard drug. On 0th and 11th day, blood was collected by retro-orbit plexus.Results: In this model blood glucose levels were determined on 0th and 11th days and MEAB significantly reduced the blood glucose levels in diabetic rats. The effect of MEAB on serum lipid profile such as total cholesterol (TC), triglycerides (TGs), low-density lipoprotein (LDL), very LDL (VLDL), and high-density lipoprotein (HDL) was also measured on the 11th day in the diabetic rats. Significant reduction in TC, TGs, LDL, and VLDL levels and improvement in HDL level were observed in diabetic rats.Conclusion: From the results, it was found that the MEAB possess antihyperglycemic and antihyperlipidemic activities.

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