scholarly journals Ameliorative effect of Ipomoea pes-caprae ethanolic leaf extract on carbohydrate metabolizing enzymes and oxidative status in Streptozotocin-induced diabetic Wistar rats

2019 ◽  
Vol 9 (4-A) ◽  
pp. 167-175
Author(s):  
S. Suhasini ◽  
C. Elanchezhiyan ◽  
G. Chandirasegaran
Keyword(s):  
Lipid Peroxidation ◽  
Leaf Extract ◽  
Diabetic Rats ◽  
Oxidative Status ◽  
Diabetic Patients ◽  
Enzymatic Antioxidants ◽  
Metabolizing Enzymes ◽  
Liver And Kidney ◽  
Ethanolic Leaf Extract ◽  
In Cells

In diabetic patients, hyperglycemia is developed due to increased hepatic glucose production and impaired utilization of glucose in cells, which leads to oxidative stress in cells. Ipomoea pes-caprae has been widely used as an oral treatment for many diseases. The aim of the present study is to assess the effect of Ipomoea pes-caprae ethanolic leaf extract on carbohydrate metabolizing enzymes and oxidative status in Streptozotocin (STZ) induced experimental diabetic rats. Experimental diabetic rats were induced by intraperitoneal administration of 55 mg/kg b.w of STZ. Diabetic rats were treated with I. pes-caprae ethanolic leaf extract at a concentration of 300 mg/kg b.w and glibenclamide (6 mg/kg b.w) for 45 days. Diabetic rats exhibited significant (P < 0.05) decline in the activity of glucokinase and glucose-6-phosphate dehydrogenase, enzymatic antioxidants (SOD, CAT and GPx) and non-enzymatic antioxidants (GSH, vitamin E and vitamin C), while lipid peroxidation markers (LOOH and TBARS) and glucose-6-phosphatase and fructose-1, 6-bisphosphatase were found to be significantly increased. Further in diabetic rats, the histopathology of pancreas, liver and kidney showed abnormal histo-architecture. The treatment with Ipomoea pes-caprae ethanolic leaf extract notably reversed the abnormal levels in carbohydrate metabolizing enzymes, restored the oxidative status and abnormal structures in pancreas, liver and kidney to near normal levels. Keywords: Diabetes, antioxidant, lipid peroxidation, Ipomoea pes-caprae and carbohydrate metabolizing enzymes.

scholarly journals Costus afer modulates the activities of glycolytic and gluconeogenic enzymes in streptozotocin induced diabetic rats

2020 ◽  
Vol 10 (4-s) ◽  
pp. 63-70
Author(s):  
Armelle D Tchamgoue ◽  
Paul DD Dzeufiet ◽  
Jules-Roger Kuiata ◽  
Gabriel A Agbor
Keyword(s):  
Leaf Extract ◽  
Islet Cells ◽  
Blood Glucose Concentration ◽  
Ethanolic Extract ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Gluconeogenic Enzymes ◽  
Liver And Kidney ◽  
Ethanolic Leaf Extract ◽  
Costus Afer

Background: Glycolytic enzymes (Hexokinase and α-glucosidase) and gluconeogenic enzymes (Glucose-6-phosphatase and fructose-1,6-disphosphatase) control carbohydrate metabolism and hence glucose hemostasis, an important approach for the control of diabetes. The present study was carried out to evaluate the effect of hydro-ethanolic leaf extract of Costus afer on these enzymes. Materials and methods: Streptozotocin-induced diabetic rats were administered hydro-ethanolic leaf extract of Costus afer (250, 500 and 1000 mg/kg body weight) orally for 8 weeks. Effect of the extract was monitored on the decrease in glycemia and the modulatory activities on glycolytic and gluconeogenic enzymes activities. The pancreas was also immunohistochemically examined for regeneration of the Islet cells. Results: Hydro-ethanolic extract significantly decreased the blood glucose concentration, serum glucose-6-phosphatase and fructose-1,6-disphosphatase activities which were associated with an increase in the activity of hexokinase in the liver and kidney. immunohistochemical examination of the pancreas, revealed the preservation and regeneration of the islet of the Langerhans cells.  Conclusion: Thus, C. afer acts against hyperglycemia modulating the activities of glucose-6-phosphatase, fructose-1,6-bisphosphatase and hexokinase, and regeneration of β-cells.

Attenuation of erythrocyte membrane oxidative stress bySesbania grandiflorain streptozotocin-induced diabetic rats

2015 ◽  
Vol 93 (4) ◽  
pp. 385-395 ◽  
Author(s):  
Chandrabose Sureka ◽  
Thiyagarajan Ramesh ◽  
Vavamohaideen Hazeena Begum
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Blood Glucose ◽  
Erythrocyte Membrane ◽  
Osmotic Fragility ◽  
Diabetic Rats ◽  
Glycosylated Haemoglobin ◽  
Albino Rats ◽  
Enzymatic Antioxidants ◽  
Protective Effects

The aim of the present study was to investigate the protective effects of Sesbania grandiflora flower (SGF) extract on erythrocyte membrane in Streptozotocin (STZ)-induced diabetic rats. Adult male albino rats of Wistar strain, weighing 190–220 g, were made diabetic by an intraperitonial administration of STZ (45 mg/kg). Normal and diabetic rats were treated with SGF, and diabetic rats were also treated with glibenclamide as drug control, for 45 days. In this study plasma insulin and haemoglobin levels were decreased and blood glucose, glycosylated haemoglobin, protein oxidation, lipid peroxidation markers, and osmotic fragility levels were increased in diabetic rats. Moreover, erythrocytes antioxidant enzymes such as superoxide dismutase, catalase, glutathione peroxide, glutathione reductase, glutathione-S-transferase, and glucose-6-phosphate dehydrogenase activities and non-enzymatic antioxidants such as vitamin C, vitamin E, reduced glutathione (GSH), and oxidized glutathione (GSSG) levels were altered. Similarly, the activities of total ATPases, Na+/K+-ATPase, Ca2+-ATPase, and Mg2+-ATPase were also decreased in the erythrocytes of diabetic rats. Administration of SGF to STZ-induced diabetic rats reduced blood glucose and glycosylated haemoglobin levels with increased levels of insulin and haemoglobin. Moreover, SGF reversed the protein and lipid peroxidation markers, osmotic fragility, membrane-bound ATPases activities, and antioxidant status in STZ-induced diabetic rats. These results suggest that SGF could provide a protective effect on diabetes by decreasing oxidative stress-associated diabetic complications.

scholarly journals EVALUATION OF ANTIDIABETIC AND ANTIOXIDANT EFFECTS OF ETHANOLIC LEAF EXTRACT OF ERYTHRINA ABBYSINICA LAM, EX DC

2018 ◽  
Vol 11 (8) ◽  
pp. 300
Author(s):  
Kamadyaapa Davie Rexon ◽  
Gondwe Mavuto Masopera ◽  
Shauli Mathulo ◽  
Sewani Rusike Constance ◽  
Nkeh Chungag Benedicta
Keyword(s):  
Blood Glucose ◽  
Leaf Extract ◽  
Antioxidant Properties ◽  
Diabetic Rats ◽  
Control Group ◽  
Oral Glucose ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Ethanolic Leaf Extract ◽  
Antioxidant Effects

  Objective: This study was conducted to scientifically evaluate the antidiabetic and antioxidant effects of ethanolic leaf extract of Erythrina abbysinica (EEA).Methods: Acute and sub-chronic effects of EEA at 100, 200, and 400 mg/kg/bwt and glibenclamide (GL) at 5 mg/kg/bwt. were evaluated in both normal and streptozotocin (STZ)-induced diabetic male Wistar rats (250–300 g). The acute studies were performed using oral glucose tolerance test (OGTT). In sub-chronic studies, animals were orally administered with EEA and GL daily for 6 w. Brine shrimp assay was used to determine the toxicity of EEA. 1, 1-diphenyl-2-picrylhydrazyl, ferric reducing capacity of plasma, and thiobarbituric acid reactive substances assays were used to determine antioxidant properties of EEA.Results: Following OGTT, EEA significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in both normal and STZ-induced diabetic rats when compared with positive and negative control counterparts at all-time points, whereas GL significantly (p<0.05) decreased blood glucose only in normal rats but not in diabetic rats. Daily, oral administration of EEA for 6 w significantly (p<0.05) and dose-dependently (100, 200, and 400 mg/kg/bwt) decreased blood glucose levels in STZ-induced diabetic rats when compared with the diabetic control group. EEA revealed weak toxicity with a lethal concentration50 value of 997 μg/ml). Furthermore, EEA showed significant free radical scavenging, total antioxidant, and anti-lipid peroxidative capacities.Conclusion: The study has shed more light on the scientific basis for the use of E. abbysinica in management of diabetes in some communities of Eastern Cape of South Africa.

scholarly journals Extraction of Phenolic Compounds from Tabernaemontana catharinensis Leaves and Their Effect on Oxidative Stress Markers in Diabetic Rats

Molecules ◽  
2020 ◽  
Vol 25 (10) ◽  
pp. 2391 ◽  
Author(s):  
Rafael Sari ◽  
Paula Conterno ◽  
Leticia Dangui da Silva ◽  
Vanderlei Aparecido de Lima ◽  
Tatiane Luiza Cadorin Oldoni ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Phenolic Compounds ◽  
High Performance ◽  
Principal Component ◽  
Antioxidant Defense System ◽  
Diabetic Rats ◽  
Total Phenolic ◽  
Antioxidant Enzyme Activities ◽  
Protein Thiols ◽  
Liver And Kidney

The aim of this study was to evaluate the most effective extraction condition (temperature, solvent type and time) for recovery of high-value phytochemicals present in the Tabernaemontana catharinensis leaves (TC) and to assess their effect on biochemical parameters in streptozotocin-induced diabetic rats. The extraction of phenolic compounds from TC using a factorial design (FD) 2³, high performance liquid chromatography (HPLC), response surface methodology (RSM) and principal component analysis (PCA) were studied. It was found that the optimal conditions for extraction of phenolics were higher temperature (65 °C) and time (60 min) using ethanol as extractor solvent. In this condition of extraction (A8), total phenolic compounds (TPC) and antioxidant activity (AA) were determined. Additionally, this extract was used to evaluate their effect on antioxidant enzyme activities (superoxide dismutase (SOD) and catalase (CAT)) as well as lipid peroxidation (LP) and protein thiols level (PSH) in the liver and kidneys of normal and diabetic rats. As result, T. catharinensis extract presented TPC content of 23.34 mg EAG/g (equivalent gallic acid) and AA of 34.26 μmol Trolox/g. Phenolic acids (ferulic acid and coumaric acid) and flavonoids (quercetin, rutin and pinocembrin) could be recovered and identified by HPLC. This study indicated an important role of the T. catharinensis extract on free radical inactivation and on the antioxidant defense system in diabetic rats. In fact, the use of T. catharinensis extract restored the normal activity of SOD (p < 0.05) and suppressed malondialdehyde levels in liver and kidney tissues. Thus, the T. catharinensis extract, rich in phenolic compounds, can be responsible for the recover the enzymatic changes in the liver and kidney tissues provoked by diabetes in rats. In addition, the lipid peroxidation rate decreased in the diabetic rats treated with T. catharinensis.

scholarly journals Antioxidant and Antidiabetic Effect of Hibiscus rosasinensis Flower Extract on Streptozotocin Induced Experimental Rats-a Dose Response Study

2011 ◽  
Vol 3 (4) ◽  
pp. 13-21 ◽  
Author(s):  
Mirunalini SANKARAN ◽  
Arulmozhi VADIVEL
Keyword(s):  
Diabetic Rats ◽  
Antioxidant Effect ◽  
Dependent Manner ◽  
Enzymatic Antioxidants ◽  
Control Groups ◽  
Metabolizing Enzymes ◽  
Detailed Mechanism ◽  
Flower Extract ◽  
Dose Response Study ◽  
Dose Dependent

The present study was designed to evaluate the antidiabetic and antioxidant effect of Hibiscus rosasinensis against streptozotocin induced diabetic rats. Streptozotocin (STZ) was administered as a single dose (40 mg/kg) to induce diabetes. The hypoglycemic activity of Hibiscus rosasinensis extract (HRSEt) was investigated in a dose dependent manner such as (125, 250 and 500 mg/kg bwt) by evaluating various biochemical parameters. The levels of blood glucose, carbohydrate metabolizing enzymes, TBARS, enzymatic and non-enzymatic antioxidants and lipid profiles were found to be significantly increased in diabetic rats when compared to control groups. Administration of extract in the treated groups showed altered changes in the above mentioned parameters and found that among the three doseses, 250 mg/kg showed best result when compared to other two doses. HRSEt possess antioxidant, hypoglycemic and hypolipidemic activity against streptozotocin induced diabetic rats. However the detailed mechanism(s) of action will require elucidating in further studies.

Influence of Loranthus micranthus on hepatic and renal antioxidant status and impaired glycolytic flux in streptozotocin-induced diabetic rats

2018 ◽  
Vol 29 (5) ◽  
pp. 447-461 ◽  
Author(s):  
Azubuike P. Ebokaiwe ◽  
Omamuyovwi M. Ijomone ◽  
Oscar Edeh ◽  
Ifebunachi Oteh ◽  
David E. Ebuka
Keyword(s):  
Glucose Metabolism ◽  
Antioxidant Status ◽  
Leaf Extract ◽  
Diabetic Rats ◽  
Glycolytic Flux ◽  
Traditional Use ◽  
Metabolism Enzymes ◽  
Liver And Kidney ◽  
Treatment Of Diabetes

Abstract Background The use of Loranthus micranthus in folklore medicine for treatment of diabetes and its associated complications is a common practice around the world. The present study investigated this traditional affirmation by in vivo investigation into the effect of L. micranthus leaf extract on hepatic and renal, oxidative status and glucose metabolism in streptozotocin (STZ)-induced diabetic rats. Methods Diabetes mellitus was induced in adult male Wistar rats by intraperitoneal injection of STZ (60 mg/kg). The diabetic rats were thereafter treated orally once per day with 5 mg/kg gilbenclamide or L. micranthus leaf extract (100 or 200 mg/kg) and monitored for 14 days. Clinical observations, plasma biochemistry, hormonal profile, oxidative stress parameters, glucose metabolism enzymes and histopathologic examination of the liver and kidney were evaluated to monitor treatment-related effects of L. micranthus leaf extract in STZ-induced diabetic rats. Results Loranthus micranthus leaf extract administration significantly ameliorated hyperglycemia-mediated damage by decreasing the blood glucose level (45.9% and 84.7% on days 7 and 14 posttreatment, respectively), enhancing the antioxidant status, inhibiting lipid peroxidation and improving the architecture of the liver and kidney in STZ-induced diabetic rats. Furthermore, intervention of L. micranthus leaf extract restored the liver and kidney function biomarkers and increased the plasma levels of triiodothyronine and thyroxine to normal control in STZ-induced diabetic rats. Conclusions The findings from this investigation provide credible scientific support for the traditional use of L. micranthus leaf extract in the treatment of diabetes and its associated complications.

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