scholarly journals La resistencia antimicrobiana en Escherichia coli aislada de canales y heces bovinas de rastros en el centro de México

2020 ◽  
Vol 11 (4) ◽  
pp. 991-1003
Author(s):  
Vicente Vega Sánchez ◽  
Martín Talavera Rojas ◽  
Jeannette Barba León ◽  
Andrea Paloma Zepeda Velázquez ◽  
Nydia Edith Reyes Rodríguez
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Risk Factor ◽  
Antimicrobial Resistance ◽  
Intestinal Microbiota ◽  
Genetic Relationship ◽  
Public Health Problem ◽  
Central Mexico ◽  
E Coli ◽  
Genetic Character

Escherichia coli is an important microorganism as an intestinal microbiota of animals and humans, so its presence in food serves as an indicator of possible fecal contamination; some strains can cause disease, mainly due to the consumption of water and animal food contaminated. The objective of this study was to detemine the resistance to antimicrobials and the genetic character of E. coli present in the carcasses and feces of bovines killed in slaugtherhouses, and to know the epidemiological panorama in Mexico. The study was carried out in 32 strains in three municipal slaugtherhouses (A, B and C) obtained from bovines in Central Mexico; their resistance profile and their genetic relationship between the different isolates were analyzed by genotyping with the enzyme XbaI-PFGE; The dendrogram was constructed using the coefficient of similarity of Dice with a tolerance of 1.5 %. It is observed that 75 % (24/32) of the isolates show resistance to some antibiotic, 84.3 % (27/32) have an intermediate profile and 12.5 % (4/32) are sensitive to all antibiotics, the 28.1 % (9/32), were MDR; 27 PFGE and pulsetypes will be identified; 7 clusters were formed with 2 or more isolates (A-F and I) and two integrated with a strain (G and H). This study shows a diversity antimicrobial resistance present in cattle carcasses and feces in Mexico, which is a risk factor and a public health problem.

Detection of Antibiotic Resistance in Escherichia coli Strains: Can Fish Commonly Used in Raw Preparations such as Sushi and Sashimi Constitute a Public Health Problem?

2019 ◽  
Vol 82 (7) ◽  
pp. 1130-1134 ◽  
Author(s):  
VANESSA SILVA ◽  
JOÃO NUNES ◽  
ANÍCIA GOMES ◽  
ROSA CAPITA ◽  
CARLOS ALONSO-CALLEJA ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Health Problem ◽  
Antibiotic Resistance Genes ◽  
Public Health Problem ◽  
Commercial Fish ◽  
E Coli ◽  
Raw Fish

ABSTRACT We studied 50 antimicrobial resistance mechanisms associated with Escherichia coli strains isolated from five species of commercial fish to evaluate the carriage and type of extended-spectrum β-lactamases (ESBLs) in these isolates. One hundred fifty samples of fresh raw fish were collected from large supermarkets and fish markets in northern Portugal during 2017. Thirty samples from each species were screened for the presence of E. coli and ESBL-producing E. coli. The samples were spread onto Levine plates with or without cefotaxime (2 μg/mL). The sensitivity of the isolates was determined for 16 antibiotics. The resistance and virulence genes were investigated by PCR. From the 150 samples, 45 E. coli isolates were recovered, and 3 of these isolates produced ESBL. All of these ESBL-producing isolates were resistant to β-lactams and harbored the blaCTX-M-1 and blaTEM genes, and all belonged to phylogenetic group A. One ESBL-producing E. coli isolate also was resistant to tetracycline and harbored the tet(A) gene, and another isolate was resistant to streptomycin and harbored the aadA gene. The remaining E. coli isolates were resistant to individual or combinations of the following antibiotics: tetracycline (16 isolates), sulfamethoxazole-trimethoprim (10 isolates), streptomycin (9 isolates), ampicillin (8 isolates), and chloramphenicol (2 isolates). The blaTEM, tet(A), aadA, and cmlA genes and combinations of sul genes were detected among most isolates that were resistant to ampicillin, tetracycline, streptomycin, chloramphenicol, and sulfamethoxazole-trimethoprim, respectively. The unmonitored and excessive use of antibiotics in aquatic environments promotes the development and propagation of antimicrobial resistance, and fish can be reservoirs of antibiotic resistance genes, which can easily be transmitted to humans through the consumption of raw fish, creating a public health problem. HIGHLIGHTS

scholarly journals Molecular characterization and antimicrobial resistance of potentially human‐pathogenic Escherichia coli strains isolated from riding horses

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Pouya Reshadi ◽  
Fatemeh Heydari ◽  
Reza Ghanbarpour ◽  
Mahboube Bagheri ◽  
Maziar Jajarmi ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Health Concern ◽  
Zoonotic Potential ◽  
Drug Resistant ◽  
Public Health Concern ◽  
E Coli ◽  
Pathogenic Escherichia Coli ◽  
Amoxicillin Clavulanic Acid

Abstract Background Transmission of antimicrobial resistant and virulent Escherichia coli (E. coli) from animal to human has been considered as a public health concern. This study aimed to determine the phylogenetic background and prevalence of diarrheagenic E. coli and antimicrobial resistance in healthy riding-horses in Iran. In this research, the genes related to six main pathotypes of E. coli were screened. Also, genotypic and phenotypic antimicrobial resistance against commonly used antibiotics were studied, then phylo-grouping was performed on all the isolates. Results Out of 65 analyzed isolates, 29.23 % (n = 19) were determined as STEC and 6.15 % (n = 4) as potential EPEC. The most prevalent antimicrobial resistance phenotypes were against amoxicillin/clavulanic acid (46.2 %) and ceftriaxone (38.5 %). blaTEM was the most detected resistance gene (98.4 %) among the isolates and 26.15 % of the E. coli isolates were determined as multi-drug resistant (MDR). Three phylo-types including B1 (76.92 %), A (13.85 %) and D (3.08 %) were detected among the isolates. Conclusions Due to the close interaction of horses and humans, these findings would place emphasis on the pathogenic and zoonotic potential of the equine strains and may help to design antimicrobial resistance stewardship programs to control the dissemination of virulent and multi-drug resistant E. coli strains in the community.

scholarly journals Antimicrobial Resistance of Escherichia coli and Salmonella isolated from Raw Retail Broiler Chickens in Zambia

2019 ◽  
Author(s):  
Elizabeth Muligisa Muonga ◽  
Geoffrey Mainda ◽  
Mercy Mukuma ◽  
Geoffrey Kwenda ◽  
Bernard Hang'ombe ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Broiler Chickens ◽  
Target Genes ◽  
Health Concern ◽  
Poultry Production ◽  
Public Health Concern ◽  
E Coli ◽  
Open Markets

Abstract Background Antimicrobial resistance (AMR) of foodborne pathogens is of public health concern, especially in developing countries like Zambia. This study was undertaken to determine the resistance profiles of Escherichia coli ( E. coli ) and Salmonella isolated from dressed broiler chickens purchased from open markets and supermarkets in Zambia.Results A total of 189 E. coli and five Salmonella isolates were isolated. Identification and confirmation of the isolates was done using Analytical Profile Index (API 20E) (Biomerieux ® ) and 16S rRNA sequencing. Antimicrobial susceptibility tests (AST) were performed using the Kirby Bauer disk diffusion technique using a panel of 10 different antibiotics and multiplex PCR was used to determine the presence of three target genes encoding for resistance: tetA, Sul1 and CTXM. AST results were entered and analyzed in WHONET 2018 software. A total of 189 E. coli and five Salmonella isolates were identified. Among the E. coli isolates, Tetracycline recorded the highest resistance of 79.4%, followed by Ampicillin 51.9%, Trimethoprim/Sulfamethoxazole 49.7%, Nalidixic Acid 24.3%, Chloramphenicol 16.4%, Cefotaxime 16.4%, Ciprofloxacin 10.1%, Colistin 7.4%, Amoxicillin/Clavulanic acid 6.9%, and Imipenem 1.1%. Two of the five Salmonella isolates were resistant to at least one antibiotic. Forty- seven (45.2%) of the isolates possessed at least one of the targeted resistance genes.Conclusion This study has demonstrated the presence of AMR E. coli and Salmonella on raw broiler chickens from both open markets and supermarkets. Such resistance is of public health concern and measures need to be put in place to regulate the use of these antimicrobials in poultry production.

Antimicrobial Resistance in Campylobacter, Salmonella, and Escherichia coli Isolated from Retail Grain-Fed Veal Meat from Southern Ontario, Canada

2011 ◽  
Vol 74 (8) ◽  
pp. 1245-1251 ◽  
Author(s):  
ANGELA COOK ◽  
RICHARD J. REID-SMITH ◽  
REBECCA J. IRWIN ◽  
SCOTT A. McEWEN ◽  
VIRGINIA YOUNG ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Antimicrobial Susceptibility ◽  
Susceptibility Testing ◽  
Tetracycline Resistance ◽  
Antimicrobial Susceptibility Testing ◽  
E Coli ◽  
Testing Resistance ◽  
Resistance Patterns

This study estimated the prevalence of Salmonella, Campylobacter, and Escherichia coli isolates in fresh retail grain-fed veal obtained in Ontario, Canada. The prevalence and antimicrobial resistance patterns were examined for points of public health significance. Veal samples (n = 528) were collected from February 2003 through May 2004. Twenty-one Salmonella isolates were recovered from 18 (4%) of 438 samples and underwent antimicrobial susceptibility testing. Resistance to one or more antimicrobials was found in 6 (29%) of 21 Salmonella isolates; 5 (24%) of 21 isolates were resistant to five or more antimicrobials. No resistance to antimicrobials of very high human health importance was observed. Ampicillin-chloramphenicol-streptomycin-sulfamethoxazole-tetracycline resistance was found in 5 (3%) of 21 Salmonella isolates. Campylobacter isolates were recovered from 5 (1%) of 438 samples; 6 isolates underwent antimicrobial susceptibility testing. Resistance to one or more antimicrobials was documented in 3 (50%) of 6 Campylobacter isolates. No Campylobacter isolates were resistant to five or more antimicrobials or category I antimicrobials. E. coli isolates were recovered from 387 (88%) of 438 samples; 1,258 isolates underwent antimicrobial susceptibility testing. Resistance to one or more antimicrobials was found in 678 (54%) of 1,258 E. coli isolates; 128 (10%) of 1,258 were resistant to five or more antimicrobials. Five (0.4%) and 7 (0.6%) of 1,258 E. coli isolates were resistant to ceftiofur and ceftriaxone, respectively, while 34 (3%) of 1,258 were resistant to nalidixic acid. Ciprofloxacin resistance was not detected. There were 101 different resistance patterns observed among E. coli isolates; resistance to tetracycline alone (12.7%, 161 of 1,258) was most frequently observed. This study provides baseline prevalence and antimicrobial resistance data and highlights potential public health concerns.

scholarly journals Antibiotic-resistant Salmonella species and Escherichia coli in broiler chickens from farms, abattoirs, and open markets in selected districts of Zambia

2020 ◽  
Vol 6 (1) ◽  
pp. 13
Author(s):  
Nelson Phiri ◽  
Geoffrey Mainda ◽  
Mercy Mukuma ◽  
Ntazana N. Sinyangwe ◽  
Luke J. Banda ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Broiler Chickens ◽  
Health Concern ◽  
Diffusion Method ◽  
Salmonella Spp ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Open Markets

Objective: Salmonella species and Escherichia coli are major bacterial enteropathogens of worldwide public health importance that cause devastating foodborne diseases, thereby contributing to increased human morbidity and mortality. Both pathogens have also been found to contribute towards the spread of antimicrobial resistance through the food chain, especially in poultry. This study aimed to determine the occurrence of antibiotic-resistant Salmonella spp. and E. coli in broiler chickens at farm level, abattoirs, and open markets in selected districts of Zambia.Methods: A cross-sectional study was undertaken in seven districts of Zambia to determine the resistance profiles of Salmonella spp. and E. coli obtained from broiler chickens at farms, abattoirs, and open markets. A total of 470 samples were collected which include; litter, cloacal swabs, and carcass swabs. Samples were inoculated into buffered peptone water and incubated for 24 hours then sub-cultured onto MacConkey and Xylose Lysine Deoxycholate agar plates. Identification of Salmonella spp. and E. coli was done using the API-20E kit and confirmation by 16S rDNA sequencing. Confirmed isolates were tested against a panel of 09 antibiotics using the Kirby-Bauer disc diffusion method and interpreted according to the Clinical Laboratory Standards Institute guidelines. Data analysis of the antibiotic sensitivity test results was done using WHONET 2018 software.Results: Overall, 4 Salmonella spp. and 280 E. coli were isolated. One of the Salmonella spp. was resistant to ampicillin (25%), amoxicillin/clavulanic acid (25%), and cefotaxime (25%). E. coli antibiotic resistance was highest to tetracycline (81.4%) and 100% susceptibility to imipenem. The antibiotic susceptibility profile revealed 75.7% (237/280) multidrug-resistant (MDR). The highest MDR profile was observed in 8.2% (23/280) isolates in which 6 out of the 9 classes of antibiotics tested were resistant. Out of the 280 isolates, 11.4% (32/280) exhibited Extensive Drug resistance (XDR).Conclusion: The study found antimicrobial resistance to E. coli and Salmonella spp. in market-ready broiler chickens which were resistant to important antibiotics and is of public health concern.

scholarly journals Antimicrobial Resistance of Escherichia coli and Salmonella isolated from Raw Retail Broiler Chicken Carcasses in Zambia

2020 ◽  
Author(s):  
Elizabeth Muligisa Muonga ◽  
Geoffrey Mainda ◽  
Mercy Mukuma ◽  
Geoffrey Kwenda ◽  
Bernard Hang'ombe ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Foodborne Pathogens ◽  
Broiler Chicken ◽  
Target Genes ◽  
Health Concern ◽  
Public Health Concern ◽  
E Coli ◽  
Genes Encoding

Abstract BackgroundAntimicrobial resistance (AMR) of foodborne pathogens is of public health concern, especially in developing countries such as Zambia. This study was undertaken to determine the antimicrobial resistance profiles of Escherichia coli ( E. coli ) and Salmonella isolated from raw retail broiler chicken carcasses purchased from open and supermarkets in Zambia.ResultsA total of 189 E. coli and five Salmonella isolates were isolated. Identification and confirmation of the isolates were done using Analytical Profile Index (API 20E) (Biomerieux ® ) and 16S rRNA sequencing. Antimicrobial susceptibility tests (AST) were performed using the Kirby Bauer disk diffusion technique using a panel of 10 antibiotics. Multiplex PCR was used to determine the presence of three target genes encoding for resistance: tet A, Sul 1 and bla CTX-M . WHONET 2018 software was used to analyse AST results. The E. coli isolates were mostly resistant to tetracycline (79.4%), ampicillin (51.9%), and trimethoprim/sulfamethoxazole (49.7%). Two of the five Salmonella isolates were resistant to at least one antibiotic. Forty- seven (45.2%) of the 104 isolates that were screened for the presence of the resistant genes possessed at least one of the targeted resistance genes.ConclusionThis study has demonstrated the presence of AMR E. coli and Salmonella on raw retail broiler chicken carcasses from open and supermarkets, which is of public health concern.

scholarly journals A Virulence and Antimicrobial Resistance DNA Microarray Detects a High Frequency of Virulence Genes in Escherichia coli Isolates from Great Lakes Recreational Waters

2006 ◽  
Vol 72 (6) ◽  
pp. 4200-4206 ◽  
Author(s):  
Katia Hamelin ◽  
Guillaume Bruant ◽  
Abdel El-Shaarawi ◽  
Stephen Hill ◽  
Thomas A. Edge ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Great Lakes ◽  
Dna Microarray ◽  
Resistance Genes ◽  
Virulence Genes ◽  
Recreational Waters ◽  
E Coli ◽  
Antimicrobial Resistance Genes

ABSTRACT Escherichia coli is generally described as a commensal species with occasional pathogenic strains. Due to technological limitations, there is currently little information concerning the prevalence of pathogenic E. coli strains in the environment. For the first time, using a DNA microarray capable of detecting all currently described virulence genes and commonly found antimicrobial resistance genes, a survey of environmental E. coli isolates from recreational waters was carried out. A high proportion (29%) of 308 isolates from a beach site in the Great Lakes carried a pathotype set of virulence-related genes, and 14% carried antimicrobial resistance genes, findings consistent with a potential risk for public health. The results also showed that another 8% of the isolates had unusual virulence gene combinations that would be missed by conventional screening. This new application of a DNA microarray to environmental waters will likely have an important impact on public health, epidemiology, and microbial ecology in the future.

Antimicrobial Resistance in Escherichia coli Isolated from Retail Milk-Fed Veal Meat from Southern Ontario, Canada

2011 ◽  
Vol 74 (8) ◽  
pp. 1328-1333 ◽  
Author(s):  
ANGELA COOK ◽  
RICHARD J. REID-SMITH ◽  
REBECCA J. IRWIN ◽  
SCOTT A. McEWEN ◽  
VIRGINIA YOUNG ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Susceptibility Testing ◽  
Grocery Stores ◽  
Southern Ontario ◽  
E Coli ◽  
Public Health Significance ◽  
Resistance Patterns ◽  
Health Significance

This study estimated the prevalence of Escherichia coli isolates in fresh retail milk-fed veal scallopini pieces obtained from grocery stores in Ontario, Canada. In addition, the prevalence and antimicrobial resistance patterns were examined for points of public health significance. One hundred fifty-three milk-fed veal samples were collected over the course of two sampling phases, January to May 2004 and November 2004 to January 2005. E. coli isolates were recovered from 87% (95% confidence interval, 80.54 to 91.83%) of samples, and antimicrobial susceptibility testing was conducted on 392 isolates. The prevalence of resistance to one or more antimicrobials was 70% (274 of 392), while the resistance to five or more antimicrobials was 33% (128 of 392). Resistance to ceftiofur (2.8%), ceftriaxone (3.6%), nalidixic acid (12%), and ciprofloxacin (3.8%) alone or in combination was observed. Eighty-five resistance patterns were observed; resistance to tetracycline only (7.4%) was observed most frequently. Individual antimicrobial resistance prevalence levels were compared with grain-fed veal and retail beef data from samples collected in Ontario. In general, resistance to individual antimicrobials was observed more frequently in E. coli isolates from milk-fed veal than in isolates from grain-fed veal and beef. Resistance to one or more antimicrobials and to five or more antimicrobials in E. coli isolates was more frequent in isolates from milk-fed veal than in isolates from grain-fed veal and beef. This study provides baseline data on the occurrence of resistance in E. coli isolates from milk-fed veal that can be compared with data for other commodities. Additionally, E. coli resistance patterns may serve as an indicator of antimicrobial exposure.

scholarly journals Prevalence of diarrhoeagenic Escherichia coli in children from León, Nicaragua

2009 ◽  
Vol 58 (5) ◽  
pp. 630-637 ◽  
Author(s):  
Samuel Vilchez ◽  
Daniel Reyes ◽  
Margarita Paniagua ◽  
Filemon Bucardo ◽  
Roland Möllby ◽  
...  
Keyword(s):  
Public Health ◽  
Escherichia Coli ◽  
Developing Countries ◽  
Infant Mortality ◽  
Health Problem ◽  
Public Health Problem ◽  
Diarrhoeal Disease ◽  
Severe Diarrhoea ◽  
E Coli ◽  
Faecal Samples

Diarrhoeal disease is a public health problem worldwide, mostly affecting children in developing countries. In Nicaragua, diarrhoea is the second greatest cause of infant mortality. During the period March 2005 to September 2006, a total of 526 faecal samples from children aged 0–60 months (381 with and 145 without diarrhoea) from León, Nicaragua, were studied. In order to detect five different diarrhoeagenic Escherichia coli pathotypes simultaneously [enterotoxigenic E. coli (ETEC), enteroaggregative E. coli (EAEC), enteropathogenic E. coli (EPEC), enterohaemorrhagic E. coli (EHEC) and enteroinvasive E. coli (EIEC)], a mixture of eight primer pairs was used in a single PCR. At least one diarrhoeagenic E. coli pathotype was detected in 205 samples (53.8 %) of the diarrhoea group and in 77 samples (53.1 %) in the non-diarrhoea group. ETEC was detected significantly more often in children with diarrhoea (20.5 %) than in children without diarrhoea (8.3 %) (P=0.001). Atypical EPEC, EIEC and EAEC were detected with slightly lower frequencies in children with (16.0, 0.8 and 27.8 %, respectively) than in children without (20.7, 1.4 and 33.1 %, respectively) diarrhoea. EHEC was only detected in children with diarrhoea (2.1 %). In conclusion, ETEC continues to be an important agent associated with diarrhoea in children from León, Nicaragua. Although not very frequent, the only findings that were 100 % associated with diarrhoea were ETEC estA (4.7 %) and EHEC (2.1 %). Nevertheless, EAEC and EPEC were also frequent pathotypes in the population under study. In children with severe diarrhoea, more than half had EAEC, ETEC or EPEC, and EAEC was the most prevalent pathotype.

Antimicrobial resistance patterns and risk factors for cloacal Escherichia coli in chickens in central Tanzania

2021 ◽  
Vol 36 (1) ◽  
pp. 15-22
Author(s):  
C.A. Newberry ◽  
J.K. Lane ◽  
B.A. Byrne ◽  
M. Mwanzalila ◽  
T. Hamza ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Risk Factor ◽  
Antimicrobial Resistance ◽  
Significant Risk ◽  
Antimicrobial Drug ◽  
Educational Outreach ◽  
Diffusion Method ◽  
Flock Size ◽  
Antimicrobial Drugs ◽  
E Coli

Antimicrobial resistance is a serious and escalating threat to global health. This study established a baseline antimicrobial resistance profile and prevalence for cloacal Escherichia coli in chickens in central Tanzania. Animal husbandry practices, flock size, and chicken breed were recorded for risk factor evaluation to elucidate potential drivers of resistance across populations. Cloacal samples were collected from poultry in Iringa, Tanzania, and E. coli isolates cultured were then tested for susceptibility to seven medically important antimicrobial drugs: ampicillin, amoxicillin-clavulanate, cefoxitin, enrofloxacin, gentamicin, sulfamethoxazole-trimethoprim, and tetracycline using the Kirby-Bauer disk diffusion method. Over 94% of the 59 E. coli isolates tested were resistant to at least one antimicrobial drug, and over 61% of isolates were resistant to three or more classes of antimicrobial drugs. The highest prevalence of resistance found was to tetracycline and sulfamethoxazole-trimethoprim (88% and 86%, respectively), which were used regularly on many sampled chickens. Previous antimicrobial usage emerged as the only significant risk factor associated with increased detection of multi-drug resistant E. coli. Further surveillance and educational outreach about antimicrobial resistance and stewardship is recommended to reduce antimicrobial drug use and to limit the potential spread of resistance to antimicrobial drugs in Tanzania.

Sign in / Sign up

Export Citation Format

Share Document