UJI AKTIVITAS ANTIBAKTERI KRIM ASAM LAURAT TERHADAP Staphylococcus aureus ATCC 25923 DAN Pseudomonas aeruginosa ATCC 27853

Lauric acid is a saturated fatty acid found in fatty/vegetable oils, especially in palm oil. Lauric acid was reported to posses antiviral and antibacterial property. This study aims to determine the effect of the concentration variance of lauric acid in cream on the stability of the cream physical quality and antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. In this research, lauric acid concentration in cream was varied in 10%, 20%, and 30% and then tested for antibacterial activity using hole plate technique. The results of the evaluation of lauric acid cream preparation of Formula 1, Formula 2 and Formula 3 showed that the organoleptic and homogeneity tests of all formula was no difference during storage time, but the result of pH, viscosity, and dispersive power of all formula has difference during storage time (28 days). The antibacterial test results showed that the highest inhibition diameter was found in the use of lauric acid concentration of 30% for S.aureus (21.589 mm ± 0.18) and P.aeruginosa (14.25 ± 0.37 mm). Based on statistical analysis of the cream produced from the three concentrations of lauric acid, they were not stable because there are significant differences in pH, viscosity, and dispersive power during storage time (28 days). Keywords: lauric acid, antibacterial cream, Staphylococcus aureus, Pseudomonas aeruginosa.

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Formulasi Sediaan Krim Anti Jerawat Ekstrak Etanol Biji Pepaya Muda (Carica papaya L.) Serta Uji Aktivitasnya Terhadap Bakteri Pseudomonas aeruginosaATCC 27825 dan Staphylococcus aureusATCC 25923 (Anti Acne Cream Formulation for Young Papaya Seed (Carica papaya L.) Ethanol Extract and Activity Test Against Pseudomonas aeruginosa ATCC 27825 and Staphylococcus aureus ATCC 25923)

MEDULA ◽

10.46496/medula.v7i1.11493 ◽

2020 ◽

Vol 7 (1) ◽

Author(s):

Nuralifah Nuralifah ◽

Feri Indradewi ◽

Parawansah Parawansah ◽

Satriana Nasrun

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Carica Papaya ◽

Ethanol Extract ◽

Inhibition Zone ◽

Diffusion Method ◽

Homogeneity Test ◽

Papaya Seeds ◽

Dispersive Power

Background: Young papaya seeds (Carica papaya L.) contained terpenoids, alkaloids karpain and flavonoids that have been examined has antibacterial activity. Purpose: This study aims to obtain anti-acne cream from extract of young papaya seeds (Carica papaya L.) that possess antibacterial activity against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27825. Methods: Extract of young papaya seeds (Carica papaya L.) was obtained by maceration process using ethanol 96% and the antibacterial activity of extract conducted by well diffusion method. then the extract formulated into anti acne cream dosage and then tested to characteristic properties including pH test, dispersive power test, organoleptic test, homogeneity test, viscosity test and irritation test. Result: The results showed anti acne creams of extract young papaya seeds (Carica papaya L.) produced qualified standard and did not cause irritation. Conclusion: Anti acne cream containing extract concentration of 10% can inhibited bacteria of S. aureus ATCC 25923 with inhibition zone of 17,5 mm and P. aeruginosa ATCC 27825 with inhibition zone of 19,3 mm.Keyword: seeds of papaya (Carica papaya L.), anti acne creamLatar Belakang: Biji pepaya muda (Carica papaya L) mengandung terpenoid, alkaloid karpain dan flavonoid, yang telah diteliti memiliki aktivitas antibakteri. Tujuan: Penelitian ini bertujuan untuk memperoleh sediaan krim anti jerawat dari ekstrak biji pepaya muda (Carica papaya L) yang memiliki aktivitas anti bakteri terhadap bakteri Staphylococcus aureus ATCC 25923 dan Pseudomonas aureginosa ATCC 27825. Metode: Ekstrak biji pepaya muda (Carica papaya L) diperoleh melalui proses maserasi menggunakan pelarut etanol 96%, serta uji aktivitas antibakteri ekstrak dilakukan dengan metode sumuran. Ekstrak selanjutnya diformulasikan menjadi sediaan krim anti jerawat dan dilakukan uji karakterisasi meliputi uji pH, uji daya sebar, uji organoleptik, uji homogenitas, uji viskositas dan uji iritasi. Hasil: Hasil pengamatan menunjukkan sediaan krim anti jerawat ekstrak biji pepaya muda (Carica papaya L) yang dihasilkan memenuhi syarat standar nilai pH, daya sebar dan viskositas. Hasil uji iritasi memperlihatkan formula krim tidak menimbulkan iritasi. Simpulan: Sediaan krim anti jerawat dengan konsentrasi ekstrak 10% dapat menghambat bakteri S.aureus ATCC 25923 dengan hambatan sebesar 17,5 mm dan P. aeruginosa ATCC 27825 dengan hambatan sebesar 19,3 mm.Kata Kunci: biji pepaya muda (Carica papaya L), krim anti jerawat

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Antibacterial Activity of Desert Truffles from Saudi Arabia Against Staphylococcus aureus and Pseudomonas aeruginosa

International Journal of Medicinal Mushrooms ◽

10.1615/intjmedmushrooms.v19.i2.30 ◽

2017 ◽

Vol 19 (2) ◽

pp. 121-125 ◽

Cited By ~ 6

Author(s):

Domenico Schillaci ◽

Maria Grazia Cusimano ◽

Stella Maria Cascioferro ◽

Vita Di Stefano ◽

Vincenzo Arizza ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Saudi Arabia ◽

Desert Truffles

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Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

Phytothérapie ◽

10.3166/phyto-2019-0150 ◽

2018 ◽

Vol 16 (S1) ◽

pp. S155-S163 ◽

Cited By ~ 2

Author(s):

S. Mehalaine ◽

O. Belfadel ◽

T. Menasria ◽

A. Messaili

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Rosmarinus Officinalis ◽

Salvia Officinalis ◽

Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

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Complexes of Cu (II) with a-Ketoglutaric Acid and 1- (o-tolyl) Biguanide Synthesis, characterization and biological Activity

Revista de Chimie ◽

10.37358/rc.19.10.7605 ◽

2019 ◽

Vol 70 (10) ◽

pp. 3603-3610

Author(s):

Madalina Mihalache ◽

Cornelia Guran ◽

Aurelia Meghea ◽

Vasile Bercu ◽

Ludmila Motelica ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Antibacterial Activity ◽

Biological Activity ◽

Candida Albicans ◽

Antifungal Activity ◽

Copper Complexes ◽

Substrate Adhesion ◽

Inert Substrate

The three copper complexes having a-ketoglutaric acid (H2A) and 1- (o-tolyl) biguanide (TB) ligands have been synthesized and characterized. The proposed formulas for these complexes are: [Cu(TB)(HA)]Cl (C1), [Cu(TB)(HA)CH3COO]�H2O (C2) and [Cu(TB)(HA)](NO3) (C3) where HA represents deprotonated H2A. The complexes obtained were tested for antibacterial activity against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853, antifungal activity on Candida albicans ATCC 10231 and antitumor activity on HeLa tumor cells. Due to the antitumor, antifungal, antimicrobial activity and inhibition of inert substrate adhesion, complexes synthesized could be used for potential therapeutic applications.

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Jabuticaba skin extracts: phenolic compounds and antibacterial activity

BRAZILIAN JOURNAL OF FOOD TECHNOLOGY ◽

10.1590/1981-6723.10817 ◽

2018 ◽

Vol 21 (0) ◽

Cited By ~ 2

Author(s):

Flávia Cíntia de Oliveira ◽

Tamara Rezende Marques ◽

Gustavo Henrique Andrade Machado ◽

Thaís Cristina Lima de Carvalho ◽

Aline Aparecida Caetano ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Listeria Monocytogenes ◽

Phenolic Compounds ◽

Methanolic Extract ◽

Ethanolic Extract ◽

Salmonella Choleraesuis ◽

Pharmaceutical Industries

Abstract The phenolic compounds from various extracts of jabuticaba skin powder (JSP) were characterized in this study, and the antibacterial activity assessed. The phenolic compounds were extracted from the JSP using four methods: a) acetone extraction - 1 g JSP: 10 mL 70% acetone, resting for 2 hours; b) aqueous extract - 1 g JSP: 15 mL water, under agitation; c) ethanolic extract - 1 g JSP: 15 mL acidified ethanol, under agitation; and d) methanolic extract - 1 g JSP: 50 mL 50% methanol, under reflux. The antibacterial activity was evaluated by the agar diffusion assay, using Escherichia coli ATCC 11229, Salmonella choleraesuis ATCC 6539, Pseudomonas aeruginosa ATCC 15442, Staphylococcus aureus ATCC 6538 and Listeria monocytogenes ATCC 19117. The ethanolic and methanolic extracts showed the highest levels of phenolic compounds, especially of cyanidin chloride, catechin and epicatechin. The extracts did not inhibit the growth of Escherichia coli and Salmonella choleraesuis, but inhibited 30% of the growth of Pseudomonas aeruginosa with an extract concentration of 250 µg mL-1. Against Staphylococcus aureus and Listeria monocytogenes the highest inhibitory effect observed was 41.8% for the ethanolic extract, followed by 36% inhibition by the methanolic extract, thus revealing the potential of these extracts as possible alternatives for use in the food and/or pharmaceutical industries.

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FLORAL EXTRACT OF Tibouchina granulosa (DESR.) COGN. PHYTOCHEMISTRY PROSPECTION, AND ANTIBACTERIAL ACTIVITY

Ipê Agronomic Journal ◽

10.37951/2595-6906.2021v5i1.6880 ◽

2021 ◽

Vol 5 (1) ◽

pp. 1-9

Author(s):

Antonio Carlos Pereira de Menezes Filho ◽

Matheus Vinícius Abadia Ventura ◽

Carlos Frederico de Souza Castro

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Enterococcus Faecalis ◽

Floral Organ ◽

Salt Formation ◽

Bacterial Strains ◽

Positive Results

Tibouchina granulosa is a species that blooms annually in several regions of Brazil. This species is still little explored in terms of phytocompounds in all organs of this plant, especially the floral organ. Flowers of T. granulosa were collected in the municipality of Rio Verde, Goiás, Brazil, in 2021. The hydroethanolic floral extract was prepared by maceration and qualitative phytochemical (colorimetric reactions and salt formation) and antibacterial analyzes performed and the results expressed in millimeters of inhibition at different concentrations in mg mL-1. Several phytochemical classes were observed with positive results, especially for alkaloids, phenolics, oxylates, saponins, carbohydrates and tannins. As for the bacterial assay, potential antibacterial activity was observed for all bacterial strains tested, except for Salmonella serovar Thyphymurium and serovar Enteritidis. Expressive inhibitions were observed for Enterococcus faecalis > Pseudomonas aeruginosa > Staphylococcus aureus and Escherichia coli at the highest concentrations between 100-50 mg mL-1. The floral extract of Tibouchina granulosa showed phytotherapeutic potential with the presence of several phytochemical groups and expressive antibacterial activity.

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Synthesis of 14-Substituted-14H-Dibenzo[a,j]Xanthene Derivatives in Presence of Effective Synergetic Catalytic System Bleaching Earth Clay and PEG-600

Catalysts ◽

10.3390/catal11111294 ◽

2021 ◽

Vol 11 (11) ◽

pp. 1294

Author(s):

Sandeep T. Atkore ◽

Giribala M. Bondle ◽

Pranita V. Raithak ◽

Vinod T. Kamble ◽

Ravi Varala ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Catalytic System ◽

Present Method ◽

Condensation Reaction ◽

Bleaching Earth ◽

Product Yield ◽

Xanthene Derivatives ◽

Peg 600

The synthesis of 14-aryl 14H-dibenzo[a,j]xanthenes is achieved by a simple condensation reaction between β-naphthol with aryl or alkyl aldehydes in an effective synergetic catalytic system created by combining basic bleaching earth clay and PEG-600. The advantages of the present method include catalyst recyclability, superior product yield, a shorter reaction time and the avoidance of hazardous reagents. Synthesized xanthene derivatives were also screened for their antibacterial activity against Staphylococcus aureus (MTCC 96) and Pseudomonas aeruginosa (Wild).

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Antibacterial Activity of Raw and Branded Honey Infused with Moringa, Ginger and Garlic on Staphylococcus aureus and Pseudomonas aeruginosa

Asian Journal of Biological Sciences ◽

10.3923/ajbs.2021.1.8 ◽

2020 ◽

Vol 14 (1) ◽

pp. 1-8

Author(s):

Agbagwa O.E. ◽

P.N. Onyishi ◽

R.A. Ikegah

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity

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Antimicrobial activity of Terminalia catappa brown leaf extracts against Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853

F1000Research ◽

10.12688/f1000research.15998.1 ◽

2018 ◽

Vol 7 ◽

pp. 1406 ◽

Cited By ~ 5

Author(s):

Ovin Qonita Allyn ◽

Eko Kusumawati ◽

Rudy Agung Nugroho

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antibacterial Activity ◽

Inhibition Zone ◽

Total Phenolic ◽

Inhibition Zone Diameter ◽

Terminalia Catappa ◽

Ethanolic Extracts ◽

High Concentrations ◽

Dried Extract

The aim of this study was to determine the effects of various concentration ofTerminalia catappabrown leaves extract which can inhibit the growth ofStaphylococcus aureusATCC 25923 andPseudomonas aeruginosaATCC 27853. The crushed-brown leaves ofTerminalia catappawas extracted using 95% ethanol, filtered, and evaporated. The driedT. catappaextract was used to identify phytochemical content qualitatively. Total phenolic and flavonoid contents were also measured quantitatively from dried extract. The dried extracts were also dissolved in sterile aquadest and serial dilutions were prepared to final concentration of 30, 60 and 90%. A disc diffusion method was used to evaluate the antibacterial activity of various concentrations of ethanol extract of brown leaves ofT. catappa. Inhibition zone diameter was measured to determine antibacterial activity. Gentamycin sulfate and distilled water were used as positive and negative controls, respectively. Dried ethanolic extract of brownT. catappaleaves contained flavonoid, quinon, phenolic, triterpenoid, and tannin. A total of 208.722 mg gallic acid equivalent/g extract of total phenolic and 35.7671 mg quercetin equivalent/g extract of total flavonoid were also found in the dried extract. The inhibition zone diameters of ethanolic extracts ranged from 1.73 to 9.06 mm (S. aureus) and from 1.83 to 6.5 mm (P. aeruginosa). The higher concentration of extract, the wider the inhibition zone diameters for both bacteria.P. aeruginosawas more resistant to high concentrations of extract (90%) thanS. aureus. Ethanolic extracts of the brown leaves ofT. catappahad different antibacterial effects againstS. aureusandP. aeruginosa. The higher the concentration of extract, the wider the inhibition zone diameter for both bacteria.P. aeruginosawas more resistant to high concentrations of ethanolic extracts of the brown leaves ofT. catappa.

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