scholarly journals Formulasi Dan Uji Aktivitas Emulgel Minyak Ikan Gabus (Channa striata) Sebagai Penyembuh Luka Bakar

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Nofriyanti ◽  
Novia Sinata ◽  
Aida Mistawati
Keyword(s):  
Wound Healing ◽  
Statistical Tests ◽  
Healing Process ◽  
Physical Stability ◽  
Wound Healing Process ◽  
Omega 3 ◽  
Globule Size ◽  
Omega 6 ◽  
Channa Striata ◽  
Ph Range

Burns often occur in various daily activities, second-degree burns are the most common. Snakehead oil (Channa striata) contains fatty acids such as omega-3 and omega-6 which can accelerate the wound healing process. This study aims to determine the physical stability and effectiveness of emulgel preparations containing snakehead oil for 8 weeks of storage of the wound healing process, which was given topically to 5 groups of treated mice.Second degree burns were made on the skin of mice, then the wound was smeared with emulgel preparations and the healing process was observed for 21 days. Cork fish oil was formulated in several concentrations; F1 (5%), F2 (10%) and F3 (15%). Organoleptic datas showed that all formulas formed as semisolid dosage form with a pale yellow color and distinctive smell of snakehead oil. The emulgel has homogenous state, not irritating effect and pH range of 5,7 to 5,3. The dosage which has a concentration of 15% gives the smallest spread but has the longest adhesion. In the Freeze and Thaw stability test conducted at 40C and 400C the dosage remains stable with an increase in globule size. Data analysis of the effectiveness of burn healing was done by two-way ANOVA statistical tests and continued with the Tukey test. The independent variable is the treatment group and the recovery time of mice while the dependent variable is the diameter of the burn. Statistical results showed that all formulas differed significantly (p <0.05) against negative controls indicating that they had effectiveness in wound healing. F3 has a better effectiveness of healing burns on the 15th day with 100% healing percent compared with F1 and F2 on the 21th day.

scholarly journals The Effectiveness Test of Oil Phase Ointment Containing Snakehead Fish (Channa striata) Extract on Open Stage II Acute Wounded Wistar Strain Male Rats

2017 ◽  
Vol 22 (2) ◽  
pp. 97 ◽  
Author(s):  
Fransisca Daisa ◽  
Mohamad Andrie ◽  
Wintari Taurina
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Stage Ii ◽  
Male Rats ◽  
Wound Healing Process ◽  
Omega 3 ◽  
Significant Difference ◽  
Healing Power ◽  
Channa Striata

The snakehead (Channa striata) contained fatty acids omega-3 and omega-6 are useful as nutrients in accelerating the wound healing process. This study aims to determine the effectiveness of the oil phase extract ointment snakehead in the healing of acute wounds open stage II. Testing the effectiveness of wound healing using 6 groups: normal, gel bioplacenton (positive control), ointment base (negative control), ointments snakehead extract oil phase concentration of 10; 20 and 40% of the 4 rats with acute wounds open stage II and observation until day 16 injury. The area of the wound was measured with the image J program Macbiophotonic, calculated% healing power, and AUC values. A statistical test to the total AUC values per rat with SPSS for Windows 22.0 program using One Way ANOVA and Post-hoc LSD test. The results showed a significant difference in the negative group with ointment concentration of 20% (p <0.05). The treatment group phase ointment fish oil extract is effective for wound healing is best to have a concentration of 20% for percentage of wound healing power of 97.157% and the average AUC value of 865.683% of the day.

scholarly journals Esktrak ikan haruan (Channa striata) menurunkan jumlah makrofag pada fase inflamasi proses penyembuhan luka (Extract of haruan (Channa striata) decreases macrophages count in inflammation phase of wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 6
Author(s):  
Putri Sri Hartini ◽  
Nurdiana Dewi ◽  
Lisda Hayatie
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Wistar Rats ◽  
Healing Process ◽  
Positive Control ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Post Test ◽  
Channa Striata

Haruan is a kind of freshwater fishes commonly found in South Kalimantan. Haruan extract contains nutritional substancessuch as albumin, Zn, Fe, Cu, and unsaturated fatty acid which can help accelerate wound healing. The aim of this studywas to histopathologically assess the effect of 100%, 50%, and 25%haruan extract compared to aquadest and ibuprofenon macrophages count in inflammation phase of wistar rats’ buccal mucosa wound healing. This research was a trueexperimental with post test-only with control design. Samples used were 30 wistar rats divided into 5 groups, 100%, 50%,25% haruan extract treatment groups, ibuprofen treatment group as positive control, and aquadest treatment group asnegative control. Mean macrophages counted on day 3 of 100%, 50%, 25% haruan extract treatment groups aquadest,and ibuprofenl were 2.05, 4.4, 3.9, 3.3, 2.4 respectively. In conclusion, haruan extract had a significant effect in decreasingmacrophages count in inflammation phase of wound healing.

scholarly journals Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Control Group Design ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Channa Striata ◽  
Post Hoc

Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract hassubstantial properties such as albumin, Zn,Cu, and Feto accelerate wound healing. The purpose of this study is to assessthe effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. Thisstudy was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatmentgroups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, eachtreatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of eachgroup was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant differencebetween negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increasedneocapillaries count in wound healing process.

scholarly journals Bioactive Proteins in Channa striata Promote Wound Healing through Angiogenesis and Cell Proliferation

2019 ◽  
Vol 27 (1) ◽  
pp. 48-59
Author(s):  
Soon Hong Kwan ◽  
Nur Hidayah Kaz Abdul Aziz ◽  
Mohd Nazri Ismail
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Ex Vivo ◽  
Healing Process ◽  
Signalling Pathway ◽  
Wound Healing Process ◽  
Water Extracts ◽  
Bioactive Proteins ◽  
Freeze Dried ◽  
Channa Striata

Background: Channa striata are speculated to contain bioactive proteins with the ability to enhancing wound healing. It is commonly consumed after surgery for a faster recovery of the wound. Objective: To identify the bioactive proteins and evaluate their ability in cell proliferation and angiogenesis promotion. Material and Methods: Freeze-Dried Water Extracts (FDWE) and Spray-Dried Water Extracts (SDWE) of C. striata were tested with MTT assay using EA.hy926 endothelial cell line and ex-vivo aortic ring assay. Later the proteins were fractionated and analysed using an LC-QTOF mass spectrometer. The data generated were matched with human gene database for protein similarity and pathway identification. Results: Both samples have shown positive cell proliferation and pro-angiogenic activity. Four essential proteins/genes were identified, which are collagen type XI, actin 1, myosin light chain and myosin heavy chain. The pathways discovered that related to these proteins are integrin pathway, Slit-Robo signalling pathway and immune response C-C Chemokine Receptor-3 signalling pathway in eosinophils, which contribute towards wound healing mechanism. Conclusions: The results presented have demonstrated that C. striata FDWE and SDWE protein fractions contain bioactive proteins that are highly similar to human proteins and thus could be involved in the wound healing process via specific biological pathways.

scholarly journals HUBUNGAN ANTARA TINGKAT KEPATUHAN PERAWAT TERHADAP SOP (STANDAR OPERASIONAL PROSEDUR) PERAWATAN LUKA DENGAN PROSES PENYEMBUHAN LUKA PASIEN PASCABEDAH DI RSUD dr. H. ANDI ABDURRAHMAN NOOR

2021 ◽  
Vol 2 (2) ◽  
pp. 55-64
Author(s):  
Farhandika Putra, Muhammad Suhada
Keyword(s):  
Wound Healing ◽  
Wound Care ◽  
Statistical Tests ◽  
Healing Process ◽  
Wound Management ◽  
Rank Test ◽  
Wound Healing Process ◽  
P Value ◽  
Cross Sectional ◽  
Operational Procedure

SOP is a procedure or stage that is standardized and must be passed to complete a particular work process and is part of the nurses' skills in applying their knowledge. Injuries are a break in the continuity of a tissue caused by an injury or surgical process. The wound healing process is influenced by several factors, one of which is wound management such as wound care. The aim of this study was to determine the correlation between the level of nurse obedience to SOP (Standard Operational Procedure) wound care with the wound healing process after surgery. This type of study was a correlation with using cross sectional design. The samples in this study were 20 nurses and 20 patients by using purposive sampling technique. This study was conducted in the RSUD dr. H. Andi Abdurrahman Noor Kabupaten Tanah Bumbu. The results of study indicated that the level of nurse obedience in the very obedient category was (40%) and the results of observations in the wound healing process were (55%) at the level of the category that was not good. After statistical tests by using the Spearman Rank test obtained P value of 0.032 <(0.05). The conclusion of this study, that there was correlation between the level of nurse obedience to SOP (Standard Operational Procedure) wound care with wound healing process after surgery.

scholarly journals Clinical Analysis of Channa micropeltes for Treating Wound of Diabetes Mellitus

2020 ◽  
Vol 16 (1) ◽  
pp. 1
Author(s):  
Maharani Laillyza Apriasari ◽  
Marina Arum Syahadati ◽  
Amy Nindia Carabelly
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Healing Process ◽  
Wistar Rat ◽  
Clinical Analysis ◽  
Control Group ◽  
Wound Healing Process ◽  
Significant Difference ◽  
Omega 6 ◽  
Wound Length

Abstract: Toman fish contains albumin and omega-6 fatty acid that are instrumental in the healing process of diabetes mellitus (DM) wound. People with DM usually have a prolonged wound healing process. Albumin controls the osmotic pressure, the development of granulation tissue, gives the energy to re-epithelialisation process and collagen base material. Omega-6 fatty acid interrupts phagocytosis induced by neutrophil cells. It increases the action of machropage cell, so that Toman fish can be used as an alternative in accelerating the DM wound healing process. The purpose of this research is to prove the effect of Toman fish extract at 16 mL/ KgBW rat dosage per oral on wound length and contraction on the back of wistar rat (Rattus novergiucs) with DM for 14 days. It was the experimental with a posttest only with control group design  used 12 wistar rat that were divided into 3 groups; the group of Toman fish extract with dose 16 mL/ Kg BW rat, positive control group using Haruan extract with dose 13,54 mL/ Kg BW rat and negative control group using Comfeed BR2 feed. One-way ANOVA test result for wound length and contraction (p= 0,000). The result Post-hoc Least Significant Difference test for wound length and contraction shows that there are significant differences between the group of Toman fish extract and Haruan fish extract (p= 0,000). There is also significant difference between the group of Toman fish extract and Comfeed BR2 feed (p= 0,000). There is no significant difference between the group of Haruan fish extract and Comfeed BR2 feed (p= 0,930). The research concluded that Toman fish extract at 16 mL/ Kg BW rat dosage affects the length and contraction of diabetic mellitus wound. Keywords: Channa micropeltes, clinical analysis, diabetes mellitus, wound

scholarly journals Ekstrakharuan(Channa striata)secara efektif menurunkan jumlah limfosit fase inflamasi dalam penyembuhan luka (Extract of haruan (Channa striata) decreases lymphocyte count in inflammatory phase of wound healing process effectively)

2014 ◽  
Vol 13 (3) ◽  
pp. 176
Author(s):  
Arisa Izzaty ◽  
Nurdiana Dewi ◽  
Dewi Indah Noviana Pratiwi
Keyword(s):  
Wound Healing ◽  
Lymphocyte Count ◽  
Healing Process ◽  
Simple Random Sampling ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Inflammatory Phase ◽  
Channa Striata

Haruan is river-water fish usually consumed by South Kalimantan people. Haruan extract contains albumin, Zn, Fe,Cu and unsaturated fatty acid which can act as antiinflammatory substances and accelarate wound healing. The aim ofthis study is to examine effectiveness of haruan extract on lymphocyte count in infammatory phase of wound healingprocess and compare it to ibuprofen. This study used true experimental method with post-test only control group designand simple random sampling. Samples were divided into 5 treatment groups:which were given the extract for 7 sevendays: 25% haruan extract group, 50% haruan extract group, 100% haruan extract group, negative control K(-) usingaquadest, positive control K(+) given ibuprofen suspension with dosage of 20mg/kg weight/day. Lymphocyte cells wereobserved using microscope and counted per field of view. Lymphocyte count reached its peak on day 5. Lymphocytecount means in each group were 4,2 ± 0,566; 1± 0,000; 3,8 ± 2,546; 7,1 ± 0,141; dan 7,5 ± 3,818 respectively. Two way ANOVA and post hoc LSD tests showed that lymphocyte count significantly decreased in 50% and 100% treatmentgroups (p<0,05) and has equal effect with ibuprofen in decreasing lymphocyte count. This study concluded that haruanextract significantly decreased lymphocyte count in wound healing process.

Biogenic silver nanoparticles as a more efficient contrivance for wound healing acceleration than common antiseptic medicine

2019 ◽  
Vol 366 (16) ◽  
Author(s):  
Ved Prakash Giri ◽  
Shipra Pandey ◽  
Madhuree Kumari ◽  
Shravan Kumar Paswan ◽  
Ashutosh Tripathi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Wound Closure ◽  
Healing Process ◽  
Diffusion Method ◽  
Wound Healing Process ◽  
Closure Rate ◽  
Bacterial Counts ◽  
Biogenic Silver Nanoparticles ◽  
Ph Range

ABSTRACT A simple and facile way of using biogenic silver nanoparticles (BSNP) (10–20 nm) was developed for wound healing acceleration and suppression of wound infections. The BSNP were formulated in an ointment base, and the study to accelerate the wound healing process was conducted in a rat. The pH of the BSNP ointment, pH 6.8 ± 0.5, lies in normal pH range of the human skin, with good spreadability and diffusibility. The wound closure rate, as a percentage, was highest at day 3 for a BSNP ointment-treated wound at 22.77 ± 1.60%, while in an untreated control the rate was 10.99 ± 1.74%, for Betadine 14.73 ± 2.36% and for Soframycin 18.55 ± 1.37%, compared with day 0. A similar pattern of wound closure rate was found at days 7 and 11. The antibacterial activity of BSNP was evaluated against wound-infection-causing bacteria Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli by the agar diffusion method. The total bacterial counts in the wound area were enumerated by the colony forming unit method. The lowest number of bacterial counts was found in the BSNP-treated wound compared with the other groups. BSNP treatment at 7.5% concentration enhanced migration of fibroblasts in a scratch assay. These findings reveal BSNP as an efficient contrivance for wound healing acceleration and as an eco-friendly alternative therapeutic antimicrobial agent.

scholarly journals Ekstrak ikan haruan (Channa striata) meningkatkan jumlah neokapiler pada penyembuhan luka (Extract of haruan (Channa striata) increases neocapillaries count in wound healing process )

2015 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Muhammad Reza Setiawan ◽  
Nurdiana Dewi ◽  
Ika Kustiyah Oktaviyanti
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Negative Control ◽  
Control Group ◽  
Wound Healing Process ◽  
Treatment Groups ◽  
Accelerate Wound Healing ◽  
Significant Difference ◽  
Channa Striata ◽  
Post Hoc

<p>Haruan is Kalimantan’s indigenous fish which has the potency to accelerate wound healing. Haruan extract has substantial properties suchas albumin, Zn,Cu, andFeto accelerate wound healing. The purposeof this study is to assess the effect of haruan extract on wistar rats buccal mucosa wound healing on day 1, 3, 5, and 7 histopathologically. This study was purely experimental with pretest-posttest with control group design. Samples were divided into 4 treatment groups, haruan extract 25%, 50%, 100% treatment groups and aquadest treatment group as negative control, each treatment wasperformed for 7 days. Neocapillariescount reached its peak on day 5. Mean neocapillaries scoring of each group was 12, 16.5, 18.5, 6 respectively. Two way Anova and post hoc LSD tests presented a significant difference between negative control and haruan extract groups. In conclusion, haruan extract treatment significantly increased neocapillaries count in wound healing process.</p><p>Haruan adalah ikankhas Kalimantan yangberpotensi mempercepat penyembuhanluka. Ekstrak ikan haruan mengandung zat penting seperti albumin, Zn , Cu, dan Fe untuk mempercepat penyembuhan luka. Penelitian ini dimaksudkan untuk mengetahui pengaruh ekstrak ikan haruan pada proses penyembuhan luka pada mukosa bukal tikus wistar hari ke-1, 3, 5, dan 7 secara histopatologi. Penelitian eksperimental murni dengan pretest-posttest with control group ini, meliputi 4 kelompok,yaitu kelompok hewan yang diberi ekstrak haruan selama7 hari dengan konsentrasi berturut-turut 25%, 50%, 100%, dan kelompok kontrol negatif diberi akuades. Hasilnya menunjukkan bahwa jumlah neokapiler mencapai puncak pada hari ke-5. Rerata jumlah neokapiler pada tiap kelompok berturut adalah 12; 16,5; 18,5; dan 6. Uji Anova 2-jalur dan uji post hoc LSDmenunjukkan adanya perbedaan bermakna antara kontrol negatif dan ekstrak ikan haruan. Berdasarkan hasil penelitian, disimpulkan bahwa pemberian ekstrak ikan haruan secara bermakna meningkatkan jumlah neokapiler pada proses penyembuhan luka.</p>

scholarly journals Acceleration of fibroblast number and FGF-2 expression using Channa striata extract induction during wound healing process: in vivo studies in wistar rats

2016 ◽  
Vol 49 (3) ◽  
pp. 125
Author(s):  
Gunawan Oentaryo ◽  
Istiati Istiati ◽  
Pratiwi Soesilawati
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Healing Process ◽  
Tissue Growth ◽  
Control Group ◽  
Wound Healing Process ◽  
Lower Lip ◽  
Treatment Groups ◽  
Collagen Secretion ◽  
Channa Striata

Background: Wound healing is a biological process associated with tissue growth and regeneration. Wound healing process, is important to repair damaged tissue. Wound healing process consists of coagulation and hemostasis, inflammation, proliferation, as well as remodeling phases. The process can be accelerated by taking synthetic or non synthetic drugs. One of them is Channa striata extract. The extract contains albumin, copper, and zinc, which can be assumed to increase inflammatory cell infiltration, fibroblast proliferation, and collagen secretion. Purpose: This study aimed to reveal the effects of Channa striata extracts on fibroblast number and FGF-2 expression in mucosal wound healing process of the Wistar rats’ lower lip. Method: This research was a true laboratory experimental research with randomized post test only control group design. Samples of experiment were devided to experiment and control group that consist five samples each. Experimental group was treted with Channa striata extract and ethanol at concentration of 25%, 50%, and 100%. The fibroblast number and FGF-2 expresion were examined. Result: The number of fibroblasts in the treatment groups receiving Channa striata extract at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest number of fibroblasts was found on day 3 at the concentration of 100% (p<0.05). Similarly, FGF-2 expression in the treatment groups receiving Channa striata at concentrations of 25%, 50%, and 100% was higher than in the control group. The highest expression of FGF-2 was found on day 3 at the concentration of 50% (p<0.05). Conclusion: Channa striata extract increased fibroblast number and FGF-2 expression in mucosa wound healing process.

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