scholarly journals Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Etanol Buah Okra (Abelmoschus esculentus L. Moench) dengan Metode Spektrofotometri UV-Vis

KOVALEN ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 74-80
Author(s):  
Joni Tandi ◽  
Bella Melinda ◽  
Anita Purwantari ◽  
Agustinus Widodo
Keyword(s):  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Abelmoschus Esculentus ◽  
Test Results ◽  
Fruit Extract ◽  
Total Alkaloids ◽  
Yellow Color ◽  
Test Parameters ◽  
Qualitative Test ◽  
Qualitative Assay

This study aims to determine the content of secondary metabolites and the levels of total secondary metabolites in ethanol extract of Okra (Abelmoschus esculentus L. Moench) fruit with UV-Vis Spectrophotometry method. Okra (Abelmoschus esculentus L. Moench) fruit extract was prepared by maceration method with 96% ethanol. The extract was concentrated using a rotary evaporator that followed by a qualitative test for alkaloids, flavonoids, saponins, and tannins using suitable reagents with the test parameters, while for the qualitative assay using a UV-Vis Spectrophotometry. Qualitative analysis of alkaloids using test parameters of total alkaloids equivalent to quinine, flavonoid using parameter of total flavonoids equivalent to quercetin, saponin using parameters of standard saponins total from the Quillaja Bark, and tannin using test parameters of total tannins equivalent to tannic acid. Qualitative test results indicated that the ethanol extract of Okra fruit tested positive for alkaloids content wich characterized by the orange deposition, flavonoids characterized by the formation of an orange-yellow color, a stable foam for saponins and tannins marked in black. The quantitative analysis resulted alkaloids of 2228.06 mg/gram, flavonoids of 2.79 mg/gram, saponins of 10.03 mg/gram, and tannins of 1973.27 mg/gram. Keywords: Okra fruit, secondary metabolites, UV- Vis spectrophotometry, qualitative, quantitative.

scholarly journals ANALISIS KADAR TOTAL METABOLIT SEKUNDER EKSTRAK ETANOL DAUN TAMOENJU (Hibiscus surattensis L.)

KOVALEN ◽  
2017 ◽  
Vol 3 (3) ◽  
pp. 258
Author(s):  
Astrid Natalia Alasa ◽  
Syariful Anam ◽  
Jamaluddin Jamaluddin
Keyword(s):  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Gravimetric Analysis ◽  
Test Results ◽  
Quantitative Test ◽  
Test Parameters ◽  
Qualitative Test ◽  
Uv Visible ◽  
Dark Blue

Tamoenju (Hibiscus surattensis L.) leaves are included in the malvaceae family which grows in the plateau and are known to have the antidiabetic activity that the standardization of the extract is needed to be conducted. The research aimed to discover the total level of secondary metabolites from ethanol extract. The extraction of Tamoenju leaves through maceration method with 96% ethanol was done to obtain the filtrate. The filtrate obtained was concentrated by using rotary evaporator at 40°C until the concentrated extract was obtained. The extract gained was then tested in a qualitative way with Alkaloid, Flavonoid, Saponin, and Tannin tests by using the suitable reagent for the test parameters. While in the quantitative test the method used was Gravimetric analysis on alkaloid and Saponin compounds, Permanganometry on Tannin compound, and UV-Visible Spectrophotometry on Flavonoid. The qualitative test results reveal that Tamoenju is positive for containing Alkaloid which was characterized by the presence of orange sediment, Flavonoid was characterized by the orange formation, Tannin was characterized by the dark blue and Saponin was characterized by the stable foam. The quantitative test results are Alkaloid by 305.181 g/g, Saponin by 371.112 g/g, Tannin by 55.417 g/g and Flavonoid by 14.999 mg/100 g.Keywords: Hibiscus surattensis L. , secondary metabolites, standardization

scholarly journals Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Biji Kelor (Moringa oleifera Lam.) dengan Metode Spektrofotometri UV-Vis

KOVALEN ◽  
2020 ◽  
Vol 6 (3) ◽  
pp. 230-238
Author(s):  
Tien Wahyu Handayani ◽  
Yulistien Yusuf ◽  
Joni Tandi
Keyword(s):  
Secondary Metabolites ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Test Results ◽  
Quantitative Test ◽  
Yellow Color ◽  
Test Parameters ◽  
Parameter Test ◽  
Uv Visible ◽  
Moringa Oleifera Lam

This study aims to determine the content and total levels of secondary metabolites of ethanol extract of moringa seed (Moringa oleifera Lam.). Extraction of moringa seed using the maceration method to obtain a filtrate. The filtrate obtained was concentrated using a rotary evaporator to obtain a concentrated extract. The extract was tested qualitatively for alkaloids, flavonoids, saponins, and tannins using a suitable reagent with the test parameters. The quantitative test was using UV-Visible spectrophotometry. Alkaloids using test parameters equivalent total alkaloid quinine, flavonoids use parameter test equivalent of the total flavonoids quercetin, saponin using test parameters from Quillaja total saponins and tannins quantitative bark using test parameters total tannin tannic acid equivalent. The result showed that moringa seed positive for alkaloids characterized by orange deposition, flavonoids are characterized by the formation of the yellow color orange, saponin their stable foam, and tannins are marked in black. Quantitative test results alkaloids of 916,87 µg/g, flavonoids of 0.255%, saponin of 6.367%, and tannin of 3,724.5 µg/g. Keywords: Moringa oleifera Lam., secondary metabolites, spectrophotometry

scholarly journals FORMULASI SEDIAAN KRIM A/M DENGAN VARIASI KONSENTRASI EKSTRAK ETANOL DAGING BUAH PEPAYA (Carica Papaya) MENGGUNAKAN EMULGATOR TWEEN 80 DAN SPAN 80

Media Farmasi ◽  
2020 ◽  
Vol 16 (1) ◽  
pp. 33
Author(s):  
Gita Puspita ◽  
Nining Sugihartini ◽  
Iis Wahyuningsih
Keyword(s):  
Phase Separation ◽  
Physical Properties ◽  
Carica Papaya ◽  
Physical Stability ◽  
Ethanol Extract ◽  
Tween 80 ◽  
Test Results ◽  
Fruit Extract ◽  
Extract Concentration ◽  
Span 80

Papaya (Carica papaya) contains flavonoid and phenolic compounds proven to have sunscreen and antithyrosinase activity. For this reason, there is a need to formulate them in the form of cream preparations. This study analyzes the effect of the ethanol extract concentration of papaya flesh in the form of A / M cream preparations on their physical properties. The extracts were obtained using the maceration method with 70% ethanol solvent in A / M cream preparations and concentrations of 1%, 3%, and 5% through Tween 80 and Span 80 emulgators. Creams were evaluated for physical properties, including pH, adhesion, dispersion, viscosity, and physical stability. The test results showed that an increase in the concentration of ethanol extract led to a decrease in pH (p <0.05), viscosity (p <0.05), adhesion (p <0.05), and an increase in dispersion (p <0.05). Additionally, phase separation occurs at a concentration of 5% papaya fruit extract cream. These results show that variations in the ethanol extract concentration of papaya fruit influence the physical properties of A / M cream.Keywords: Carica papaya; A / M cream; Physical Properties.Pepaya (Carica papaya) mengandung senyawa flavonoid dan fenolik  yang terbukti memiliki aktivitas sebagai tabir surya dan antitirosinase sehingga perlu diformulasikan dalam bentuk sediaan krim. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh konsentrasi ekstrak etanol daging buah papaya dalam bentuk sediaan krim A/M terhadap sifat fisik sediaan. Ekstraksi buah papaya diperoleh dengan metode maserasi dengan pelarut etanol 70% dalam sediaan krim A/M dengan konsentrasi 1% (FI), 3% (F2) Dan 5% (F3). Krim dievaluasi sifat fisik meliputi pH, daya lekat, dan daya sebar, viskositas dan stabilitas fisik. Hasil uji menunjukkan bahwa peningkatan konsentrasi ekstrak etanol buah papaya menyebabkan penurunan pH (p<0.05), viskositas (p<0.05), daya lekat (p<0.05), peningkatan daya sebar (p<0.05). Terjadi pemisahan fase pada konsentrasi 5% krim ekstrak buah papaya. Berdasarkan hasil dapat disimpulkan bahwa variasi konsentrasi ekstrak etanol daging buah papaya memiliki pengaruh terhadap sifat fisik krim A/MKata kunci : Carica papaya; krim A/M; Sifat Fisik

scholarly journals Identification of Secondary Metabolites from Ethanol Extract of Ciplukan (Physalis angulate) Leaves and Toxicity Test on Post-Larvae of Tiger Shrimp (Penaeus monodon) [Identifikasi Senyawa Metabolit Sekunder Ektrak Etanol Daun Ciplukan (Physalis angulata) dan Uji Toksisitas terhadap Sintasan Post Larva Udang Windu (Penaeus monodon)]

2019 ◽  
Vol 11 (2) ◽  
pp. 92
Author(s):  
Awaludin Awaludin ◽  
Yulma Yulma
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Toxicity Test ◽  
Egg Quality ◽  
Penaeus Monodon ◽  
Ethanol Extract ◽  
Lethal Concentration ◽  
Test Results ◽  
Tiger Prawn ◽  
Garden Plant

AbstractPhysalis angulata is a garden plant that has been widely used as a medicinal plant by the community. This plant contains secondary metabolites that can be used as medicine and nutrition enhancement. This study aims to identify the secondary metabolite compounds and their toxicity to the post-larva (PL) survival rate in tiger prawns. This study was conducted in several stages (1). P. angulata extraction using ethanol, (2) P. angulata GCMS test, (3) The identification of the secondary metabolites from P. angulata (4) LC50 test (Lethal Concentration 50) and (5) Data analysis. Based on the results of GCMS analysis, it was indicated that P. angulata contained the secondary metabolite compounds, including phenols, steroids, fatty acids, terpenoids, benzenes, and alkaloids. It is concluded that the compounds contained are presumed to be beneficial for prawn farming development in terms of disease prevention, growth acceleration, and egg quality improvement. Meanwhile, the toxicity test results by using tiger prawn larvae for 48 hours revealed that the safe concentration level used for prawn development by utilizing the cutleaf groundcherry extract was at 13.1 mg/l.AbstrakPhysalis angulata merupakan tumbuhan pekarangan yang telah banyak dimanfaatkan sebagai tumbuhan obat oleh masyarakat. Tumbuhan ini diduga memiliki metabolit sekunder yang dapat dimanfaatkan sebagai obat dan peningkatan nutrisi. Penelitian ini bertujuan untuk mengindentifikasi senyawa metabolik sekunder dan toksisitasnya terhadap sintasan post larva (PL) udang windu. Penelitian ini dilakukan dengan beberapa tahapan (1). Ekstraksi P. angulata dengan menggunakan etanol, (2) Uji GCMS P. angulata, (3) Identifikasi metabolit sekunder dari P. angulata (4) Uji LC50 (Lethal Concentration 50) dan (5) Analisis data. Berdasarkan hasil analisis GCMS menunjukkan bahwa P. angulata mengandung metabolit sekunder golongan senyawa antara lain fenol, steroid, asam lemak, terpenoid, benzene dan alkaloid. Senyawa- senyawa yang terkandung tersebut diduga dapat dijadikan sebagai pengembangan budidaya udang pada penanggulangan penyakit, percepatan pertumbuhan dan peningkatan kualitas telur. Sedangkan hasil uji toksisitas dengan menggunakan larva udang windu selama 48 jam menunjukkan bahwa konsentrasi aman yang dapat digunakan untuk pengembangan udang dengan memanfaatkan ekstrak ciplukan dengan konsentrasi 13,1 mg/l.

DAYA TAHAN ROTAN YANG DIAWETKAN DENGAN CUKA KAYU GALAM TERHADAP SERANGAN BUBUK Dinoderus minutus Farb.

2010 ◽  
Vol 2 (2) ◽  
pp. 8
Author(s):  
Evy Setiawati
Keyword(s):  
Force Feedback ◽  
Test Results ◽  
Dry Powder ◽  
Degree Of Protection ◽  
Wood Vinegar ◽  
Dinoderus Minutus ◽  
Test Parameters ◽  
Feedback Method ◽  
Reduction Percentage ◽  
Insect Attack

Rattan on frequently attacked by the powder post beetle (Tellu, 2001). The prevention of dry powder attacks is done by preservation. The increasing resistant of rattan from insect attack can be done by an environmentally friendly preservative, the Galam wood vinegar. This research  aims to determine the most effective concentration of preservative that shows the lowest attacks level of D. Farb minutus powder. The rattan used is green rattan (Calamus sp.) The concentration of preservative that are used:10%, 40%, 70% and 100%. The testing of dry powder attack  used force feedback method. The effectiveness test parameters of wood vinegar to dry powder attacks  included degree of protection Dinoderus minutus Farb. powder,  reduction percentage of rattan weight and the mortality of dry powder Dinoderus sp for toxicological testing of wood vinegar. The test results showed that the degree of protection powder in rattan growing along with the increased concentration of preservatives. The higher the concentration of  wood vinegar, the smaller the reduction of rattan weight and the higher the mortality rate of dry powder. Keywords: resistant of rattan, wood vinegar, Dinoderus minutus.

PENGARUH LAMA FERMENTASI TERHADAP KADAR FLAVONOID TOTAL PADA EKSTRAK TAPE BIJI NANGKA (Artocarpus heterophylla Lamk.)

2020 ◽  
pp. 53-61
Author(s):  
Nada Nabila Sari ◽  
S Suharyanto
Keyword(s):  
Extraction Method ◽  
Positive Sample ◽  
Total Flavonoid ◽  
Test Results ◽  
Fermentation Time ◽  
Flavonoid Compounds ◽  
Community People ◽  
Qualitative Test ◽  
Seed Extracts

Biji nangka kurang diminati oleh masyarakat sebagai bahan pangan. Biji nangka memiliki kandungan senyawa flavonoid yang dapat digunakan sebagai pengobatan. Tujuan peneliti adalah untuk mengetahui pengaruh lama fermentasi terhadap kadar flavonoid total pada ekstrak tape biji nangka (Artocarpus heterophylla Lamk.) yang dilakukan dengan alat spektrofotometer UV-Vis. Manfaat dari penelitian untuk menambah wawasan di bidang farmasi khususnya tentang ilmu kimia amami mengenai pengaruh lama fermentasi terhadap kadar flavonoid total pada ekstrak tape biji nangka (Artocarpus heterophylla Lamk.). Metode ekstraksi yang digunakan adalah metode maserasi dengan mengunakan pelarut etanol 96%. Identifikasi kualitatif mengunakan uji Shinoda, uji NaOH 10%, uji H2SO4 (pekat). Hasil uji kualitatif menunjukan sampel positif mengandung flavonoid. Kesimpulan pada hasil penelitian penetapan kadar flavonoid total yang terkandung dalam ekstrak tape biji nangka pada hari ke-0 sebesar 2,9146±0,0245 mgQE/gram ekstrak, pada hari ke-1 sebesar 3,3050±0,0240 mgQE/gram ekstrak, pada hari ke-2 sebesar 3,6829±0,0244 mgQE/gram ekstrak, dan pada hari ke-3 sebesar 4,0488±0,0244 mgQE/gram ekstrak.  Jackfruit seeds are less interest by the community (people) as food.  Jackfruit seeds containing flavonoid compounds where it can be used as a treatment.  The aim of the researchers was to determine the effect of fermentation time on total flavonoid concentration  in jackfruit fermented seed extracts with a UV-Vis spectrophotometer. The benefits of research to add insight in the field of pharmacy especially about chemistry of amami regarding effect of fermentation time on total flavonoid levels in jackfruit seed “tape” extract (Artocarpus heterophylla Lamk.). Extraction method used is the maceration method using 96% ethanol solvent. Qualitative identification using the Shinoda test, 10% NaOH test, and H2SO4 (concentrated) test. Qualitative test results showed a positive sample containing flavonoids. Conclusions on the results of the experiment  are jackfruit seed "tape" extract on day-0 was 2.9146±0.0245  mgQE/gram extract, on day-1 was 3.3050±0.0240 mgQE/gram extract, on day-2 was 3.68293±0.0244 mgQE/gram and on day-3 was 4.0488±0.024 mgQE /gram extract. The longer fermentation, total flavonoid concentration interest.  

UJI IMUNOSTIMULATOR DARI KOMBINASI EKSTRAK ETANOL HERBA BINARA (ARTEMISIA VULGARIS LINN) DAN EKSTRAK ETANOL DAUN PUCUK MERAH (SYZYGIUM OLEANA) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT PADA TIKUS JANTAN

2019 ◽  
Vol 1 (2) ◽  
pp. 22-26
Author(s):  
Romauli Anna Teresia Marbun ◽  
Aminah Syarifuddin ◽  
Montysory Silalahi ◽  
Radika Bella Fista Ginting
Keyword(s):  
Antioxidant Activity ◽  
Immune System ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Positive Control ◽  
Artemisia Vulgaris ◽  
Chemical Screening ◽  
Chemical Content ◽  
High Antioxidant Activity ◽  
New Treatments

Diseases mediated by the immune system are difficult problems to treat such as human immunodeficiency virus (HIV) and other lethal viruses. Infections that occur in normal people are generally brief and rarely leave permanent damage. Treatment of this disease requires an aggressive and innovative approach to the development of new treatments so that it requires the role of immunomodulators to improve the immune system. A substance that acts as an enhancer or immune enhancer can be obtained by using herbs that are efficacious as immunostimulants. One of the herbs used is herbal binara (Artemisia vulgaris L) which has been studied as a potential immunomodulator with high antioxidant activity. Previous research also stated that red shoots (Syzygium oleana) were studied as potential immunomodulators with high antioxidant activity. Several other species such as Syzygium samarangense have 16 flavonoida compounds which show pharmacological immunological activity. The purpose of this study was to determine the content of secondary metabolites of ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) and to determine the best dose of extract from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) can reduce the volume of swelling of mouse feet. Examination of the chemical content of secondary metabolites from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) is carried out by chemical screening and characterization of simplicia and extract. The method used is the slow type hypersensitivity method. In this test the independent variable is the secondary metabolite of ethanol extract of herb binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) with four concentrations (50, 100, 200 and 400 mg / kgBB). The positive control used by Stimuno dose is 32.5 mg / kgBB

Modeling the Tensile Behaviour of a SiC/SiC Composite by Master Curves

2000 ◽  
Author(s):  
J. Shi
Keyword(s):  
Master Curve ◽  
Ceramic Matrix Composites ◽  
Test Material ◽  
Tensile Behaviour ◽  
Matrix Composites ◽  
Test Results ◽  
Master Curves ◽  
The Past ◽  
Test Parameters ◽  
Tensile Data

Scatter in test results is common for relatively brittle materials such as ceramic matrix composites. The scatter may come from differences in material processing conditions, specimen machining/handling and from variations in test parameters for nominally the same test material. Large scatter in test results makes material modeling difficult. In the past, master curve concepts have been proposed to reduce scatter in tensile data and to interpret fatigue/creep results. In this paper, one such concept is examined in detail by applying it to the recent tensile test results of a SiC/SiC composite. It was found that the way to construct master curves did not apply to the CMC studied and thus a new master curve was developed to better represent the tensile data. In addition, the test data were analysed statistically based on the new master curve.

analysis of mechanical properties of ceiling boards based on corn cobs and coconut coir waste with latex adhesive

2021 ◽  
Vol 5 (2) ◽  
pp. 1
Author(s):  
Ety Jumiati ◽  
Ufik Eliati Tumanggor ◽  
Abdul Halim Daulay
Keyword(s):  
Mechanical Properties ◽  
Flexural Strength ◽  
Fracture Strength ◽  
Test Results ◽  
Hot Press ◽  
Optimal Result ◽  
Corn Cob ◽  
Corn Cobs ◽  
Test Parameters ◽  
Coconut Coir

<p align="center"><strong><em>Abstract</em></strong></p><p>Ceiling evelopment by utilizing waste corn cobs, coconut coir with gypsum flour an the adition of latex adhesive. Variations in the composition of the mixture of corn cobs, coconut coir, gypsum flour with latex adhesive include sample A (0:0:100:15), sample B (3:3:94:15), sample C (6:6:88:15), sample D (9:9:82:15), sample E (12:12:76:15), and sample F (15:15:70:15) with emphasis using a <em>hot press </em>and drying for 28 days. The test parameters include flexural strength and fracture strength. The test results show that sample B in the composition (3:3:94:15) is the optimal result. In this composition, the resulting ceiling board has the characteristics of a flexural strength of 3966,39 kgf/cm<sup>2</sup> and a fracture strength of 1088,6 kgf/cm<sup>2</sup>.</p><p><strong><em>Keyword :</em></strong><em> Lateks , Ceiling Board, Coconut Coir, Corn Cob</em></p><p><em> </em></p>

Durability of BFRP and Hybrid FRP Sheets under Freeze-Thaw Cycling

2010 ◽  
Vol 163-167 ◽  
pp. 3297-3300 ◽  
Author(s):  
Jia Wei Shi ◽  
Hong Zhu ◽  
Zhi Shen Wu ◽  
Gang Wu
Keyword(s):  
Mechanical Properties ◽  
Test Data ◽  
Mechanical Characteristics ◽  
Test Results ◽  
Freeze Thaw ◽  
Frp Composites ◽  
Test Parameters ◽  
The Stability ◽  
Better Than

Coupon tests were conducted to investigate the mechanical characteristics of basalt FRP (BFRP) sheet, basalt-carbon hybrid FRP sheets and the corresponding epoxy rein under the effect of freeze-thaw cycling. FRP sheets and epoxy rein coupons were subjected to up to 200 and 250 freeze-thaw cycles respectively. Test parameters included the number of freeze-thaw cycles and the types of FRP composites. Test results show that (1) BFRP sheet perform better than CFRP or GFRP sheets under high freeze-thaw cycles; (2) exposed hybrid FRP sheets not only show very little loss in mechanical properties, but also contribute to the stability of test data; (3) mechanical properties of rein epoxy decrease significantly with increasing freeze-thaw cycles.

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