Analisis Kadar Glukomanan Dari Biji Durian (Durio zeibethinus Murr) dengan Metode Spektrofotometri pada Berbagai Waktu dan Suhu Hidrolisis

Durian seeds are one type of seed that contains glucomannan. The aim is to determine glucomannan levels in the durian seed. The study was designed using a completely randomized design (CRD). The extracted glucomannan was determined by the spectrophotometry method at various temperature and hydrolysis time. The crude extract of glucomannan was hydrolyzed using 3 M HCl for 70, 80, 90, 100, and 110 minutes at 70, 80, 90, 100, and 110°C. The highest glucomannan concentration of 39.60% was obtained at a hydrolysis temperature of 100°C for 100 minutes. The analysis of the FTIR spectrophotometer shown that the vibration of the C-O-C functional group (glycoside bond) was obtained at the wavelength number of 1020.34 cm-1 which is a characteristic of glucomannan. Keywords: glucomannan, durian seed, hydrolysis, spectrophotometry

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Sensitivity Testing of Ciplukan (Physalis angulata) Rod Crude Extract on The Bacterium Pseudomonas fluorescens In Vitro

Samakia : Jurnal Ilmu Perikanan ◽

10.35316/jsapi.v10i1.233 ◽

2019 ◽

Vol 10 (1) ◽

pp. 24-30

Author(s):

Ion Tarsardo Sianturi ◽

Arief Prajitno ◽

Ellana Sanoesi

Keyword(s):

Experimental Method ◽

Crude Extract ◽

Inhibition Zone ◽

Natural Material ◽

Sensitivity Testing ◽

Randomized Design ◽

Physalis Angulata ◽

Completely Randomized Design

Diseases is a problem in aquaculture, one of which is the diseases caused by the bacteriumP.fluorescens. Antibiotics and chemicals materials themselves can give the bacterium resistance and cause harm for the environment. One alternative that can be done is using a natural material, namely the rod of ciplukan (p. angulata). The purpose of this research was to explain the effect of P. angulata on the growth of p. fluorescens. The method which can be use is experimental method by using the research design of completely randomized design (ral) with 5 treatment and 2 control with 3 repetitions. The results showed that extract of P. angulata at various dose (6,67 ppt, 13,33 ppt, 19,99 ppt, 26,66 ppt and 33,33 ppt) exhibited anti-P. fluorescens activity with inhibition zone diameters in the range of (4,06±0,07 mm - 9,63±1,61 mm). Increashing extract dose lead to increased the inhibition zone. The extract dose of 33,33 ppt exhibited best anti-P. fluorescens activity in this research. The research results show that P. angulata is proven to be able to inhibit the P. fluorescens, but to prove the effectiveness of this material, an in vivo is required.

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PENGARUH EKSTRAK KASAR BUAH PALA MYRISTICA ARGENTEA TERHADAP PERTUMBUHAN IKAN KOI CYPRINUS CAPRIO

Journal of Aquaculture and Environment ◽

10.35965/jae.v4i1.1314 ◽

2021 ◽

Vol 4 (1) ◽

pp. 7-10

Author(s):

Nuryanti Leko ◽

Sutia Budi ◽

Mardiana Mardiana ◽

Dahlifa Dahlifa

Keyword(s):

Growth Rate ◽

Crude Extract ◽

Research Method ◽

Ornamental Fish ◽

Good Effect ◽

Length Growth ◽

Weight Growth ◽

Randomized Design ◽

Test Parameters ◽

Completely Randomized Design

Ikan koi di Indonesia merupakan ikan hias favorit dan banyak digemari masyarakat luas karena tubuhnya yang mempesona dan harganya relatif tidak terlalu mahal. Ikan koi sekarang ini masih menjadi salah satu komoditas perdagangan yang cukup baik dalam bidang perikanan. Tujuan dari penelitian ini adalah untuk mengetahui Pengaruh Ekstrak Kasar Buah Pala Myristica Argentea Terhadap Pertumbuhan Ikan Koi Cyprinus Caprio. Metode penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) ,dengan empat perlakuan dan tiga ulangan yaitu perlakuan A (Dosis 5% ekstrak kasar buah pala), perlakuan B (Dosis 10% ekstrak kasar buah pala), perlakuan C (Dosis 15% ekstrak kasar buah pala) dan perlakuan D ( Dosis 0 % ekstrak kasar buah pala). Parameter uji berupa lajui pertumhuhan bobot dan pertumbuhan panjang. Hasil penelitian menunjukkan bahwa ekstrak Kasar Daging Buah Pala myristica argentea memberikan pengaruh yang baik terhadap pertumbuhan ikan ko setelah diberikan perlakuan.. Dosis yang baik untuk pertumbuhan berat dan panjang ikan koi ialah dosis 10% - 5%. Koi fish in Indonesia is a favorite ornamental fish and much-loved by the wider community because of its charming body and relatively inexpensive price. Koi fish is still one of the trade commodities that is quite good in the field of fisheries. The purpose of this study was to determine the Effect of Crude Extract of Nutmeg Myristica Argentea on the Growth of Cyprinus Caprio Koi Fish. The research method used was a completely randomized design (CRD), with four treatments and three replications, namely treatment A (5% dose of nutmeg crude extract), treatment B (10% dose of nutmeg crude extract), treatment C (15% dose of nutmeg extract). crude nutmeg) and treatment D (Dose of 0% crude extract of nutmeg). Test parameters in the form of weight growth rate and length growth. The results showed that the crude extract of the Nutmeg myristica argentea had a good effect on the growth of koi fish after being given treatment. A good dose for the growth of weight and length of koi fish is a dose of 10% - 5%

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Characteristic of Protein Hydrolyzate of Snakehead Fish (Channa striata) Head by Using the Treatment of Different Bromelin Enzyme Concentration and Hydrolysis Time

International Journal of Scientific Research in Science and Technology ◽

10.32628/ijsrst196439 ◽

2019 ◽

pp. 188-196

Author(s):

Sri Udayana Tartar ◽

M Mahendradatta ◽

Prof. Mursalim ◽

Prof. Adiansyah

Keyword(s):

Enzyme Concentration ◽

Fish Protein ◽

Food Sector ◽

Hydrolysis Time ◽

Protein Hydrolyzate ◽

Randomized Design ◽

Channa Striata ◽

Completely Randomized Design ◽

The Difference ◽

Hydrolysis Of

Snakehead fish (Channa striata) has fairly high protein content; hence it has the potential to be utilized as a fish protein hydrolyzate. Fish protein hydrolyzate has great benefits in a food sector; therefore in order to obtain functional food products from fish that meet the standards and to utilize snakehead fish head, then enzymatic hydrolyzate products are made using bromelin enzymes. The research was aimed to determine the physicochemical properties of protein hydrolysates of snakehead fish head. It uses a laboratory experimental using a Completely Randomized Design (CRD). The data obtained were analyzed using ANOVA with further testing to determine whether or not the value was influential with Tukey test. The treatment applied was the difference in protein hydrolyzate of snakehead fish head, i.e the weight of head meat of snakehead fish given the treatment of bromelin enzymes with different percentages (6, 8 and 10%) and with different hydrolysis times (8, 10 and 12 hours). The results of research showed that the best result of treatment were obtained on 10% bromelin enzyme by temperature hydrolysis of 600C for 8 hours with the criteria of a brownish-yellow protein hydrolyzate, and having a water content of 80.40 - 77.79 %, ash 0.65 - 0.12%, protein 21.11 - 18.49%, fat 0.84 - 0.65%, yield 52.22 - 43.69%, pH 7.0 - 6.7, brightness 29.93 -23.13.

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PENGGUNAAN BERBAGAI TEKANAN DAN WAKTU HIDROLISIS PADA PRODUKSI GLUKOSAMIN HIDROKLORIDA DARI KITOSAN CANGKANG BEKICOT (Achatina fulica)

KOVALEN ◽

10.22487/j24775398.2016.v2.i1.6042 ◽

2016 ◽

Vol 2 (1) ◽

Author(s):

Noviana Linawati Dewi ◽

Syaiful Bahri ◽

Jaya Hardi

Keyword(s):

Factorial Design ◽

Time Pressure ◽

Distilled Water ◽

Achatina Fulica ◽

Hydrolysis Time ◽

Glucosamine Hydrochloride ◽

Snail Shell ◽

Randomized Design ◽

Completely Randomized Design

ABSTRACT This study aims to determine the effect of pressure and time hydrolysis to produce glucosamine hydrochloride with the highest yield and the best quality of the chitosan of a snail shell. The study used a completely randomized design (CRD) factorial design consisting of 2 factors with 5 variations of hydrolysis time (40; 50; 60; 70 and 80) minutes and 2 variations of pressure (1 atm and 2 atm). Each treatment is done twice so obtained 20 experimental units. The highest glucosamine hydrochloride was obtained in hydrolysis time of 80 minutes and a pressure of 1 atm. Yield of 7,54%. Test of solubility in distilled water at 20°C was obtained of 3,79. Mol/L, while the maximum absorption of the UV-Vis with the addition of reagent Schales is at a wavelength of 420 nm. Keywords: Snails, Chitosan, Glucosamine, Hydrolysis Time, Pressure

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Respon Allelopati Gulma Ageratum conyzoides dan Borreria alata terhadap Pertumbuhan dan Hasil Tiga Varietas Kedelai (Glycine max)

Jurnal Agro ◽

10.15575/162 ◽

2015 ◽

Vol 2 (1) ◽

pp. 39-49 ◽

Cited By ~ 1

Author(s):

Abdul Karim Kilkoda

Keyword(s):

Glycine Max ◽

Root Exudates ◽

Crude Extract ◽

Growth And Yield ◽

Independent Effect ◽

Ageratum Conyzoides ◽

Yield Parameters ◽

Randomized Design ◽

Completely Randomized Design ◽

Almost All

Penelitian ini bertujuan untuk menguji pengaruh eksudat akar gulma Ageratum conyzoides dan Borerriaalatayang didalamnya terkandung alelopati tanin dan fenol terhadap pertumbuhan dan hasil tiga varietas kedelai berdasarkan ukuran benih kedelai. Percobaan dilaksanakan di Rumah kaca Fakultas Pertanian Universitas Padjadjaran, JatinangorKabupaten Sumedang, Jawa Baratpada bulan September 2014. Penelitian disusun dalam Rancangan Acak Lengkap factorial. Faktor pertama adalah ekstrak kasar gulma, yaitu; EG0 = ekstrak gulma 0 g/100 ml(kontrol), EG1 = ekstrak gulma (Ageratum + Borreria)100 (g/v), EG2 = ekstrakgulma (Ageratum + Borreria)200 (g/v), EG3 = ekstrak gulma (Ageratum + Borreria) 300 (g/v), faktor kedua yaitu ukuran bobot dan jenis kedelai; K1 = varietas Gepak Kuning (bobot 100 biji : 6,82 gram) ukuran bobot kecil, K2 = varietas Gema(bobot 100 biji : 12 gram) ukuran bobot sedang, K3 = varietas Grobogan (bobot 100 biji : 17,8 gram) ukuran bobot besar. Tidak terdapat interaksi antara ekstrak kasar gulma dengan ukuran bobot dan jenis kedelai terhadap seluruh parameter pertumbuhan dan hasil yang diamati. Terdapat pengaruh mandiri antar sesama varietas kedelai hampir pada semua parameter pertumbuhan dan hasil tanaman kedelai, kecuali parameter luas daun, dengan nilai tertinggi dihasilkan oleh varietas Groboganyang memiliki ukuran biji besar. Terdapat pengaruh mandiri dari ekstrak kasar gulma Ageratum conyzoides dan Borerriaalata pada semua parameter pertumbuhan dan hasil tanaman kedelai.This study aimed to examine the effect of root exudates of weeds Ageratum conyzoides and Borerria alata therein containing tannins and phenols as allelopathy on growth and yield of three soybean varieties with concern to size of the seed. The experiment was conducted in greenhouse of Faculty of Agriculture, Universitas Padjadjaran, Jatinangor Sumedang, West Java in September 2014. The study was set in completely randomized design factorial. First factor was the weed crude extract, namely; EG0 = weed extract 0 g / 100 ml (control), EG1 = weed extract (A. conyzoides + B. alata) 100 (g / v), EG2 = weed extract (A. conyzoides + B. alata) 200 (g / v), EG3 = weed extract (A. conyzoides + B. alata) 300 (g / v). Second factor was and type of soybean; K1 = variety Gepak Kuning (weight of 100 seeds: 6.82 gram) weight size small, K2 = variety Gema (weight of 100 seeds: 12 gram) weight size medium, K3 = variety Grobogan (weight of 100 seeds: 17.8 gram) weight size big. There was no interaction between weed crude extract and soybean type. Meanwhile, there was independent effect among varieties tested for almost all parameters observed, except for leaf width. There was also independent effect from crude extract of Ageratum conyzoides and Borerria alata on all growth and yield parameters of soybean.RESPON ALLELOPATI GULMA Ageratum conyzoides DAN Borreria alata TERHADAPPERTUMBUHAN DAN HASIL TIGA VARIETAS KEDELAI (Glycine max)<w:LsdException Locked="false" Priority="70" SemiHidden="false" UnhideWhenUsed="false" Name="Dark List

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Aktivitas Ekstrak Kasar Enzim Kolagenase dari Organ Dalam Ikan Malong (Congresox talabon) pada pH Berbeda

Jurnal Teknologi dan Industri Pertanian Indonesia ◽

10.17969/jtipi.v13i1.18587 ◽

2021 ◽

Vol 13 (1) ◽

pp. 27-30

Author(s):

Wikky Aditiya Putra ◽

Anda Rini Diharmi ◽

Rahman Karnila

Keyword(s):

Crude Extract ◽

Protein Concentration ◽

Protein Hydrolysis ◽

Biological Functions ◽

Internal Organs ◽

Fish Yield ◽

Activity Test ◽

Randomized Design ◽

Completely Randomized Design ◽

Substrate Inhibitor

Enzymes are biocatalysts that function as catalysts in biological functions. Collagenase enzymes have activities that can support product production. Enzyme activity for protein hydrolysis is influenced by several factors such as protein concentration, pH, temperature, substrate, inhibitor and activator. This study was aimed to determine the effect of differences in pH on the crude extract activity of the collagenase enzyme from the internal organs of malong fish. The study used a non-factorial completely randomized design, the treatments used were different pH (6.5, 7.5, and 8.5). This research consisted of several stages, namely sample preparation, extraction of internal organs to produce crude extract of the collagenase enzyme and test the activity of crude extract of the collagenase enzyme based on different pH. The analysis parameters was consisted of the proportion of fish yield, and the enzyme crude extract activity test at different pH. The results showed that malong fish has a proportion of meat 7,2 parts, bone 3,8, internal organs 1, skin 2,1. The yields of crude extract of collagenase enzyme pH 6.5, 7.5, and 8.5 were 60.83%, 64.09%, and 83.15%, respectively. The crude extract activity of collagenase enzyme from the internal organs of malong fish at pH 6.5, 7.5 and 8.5 was resulted in 0.293 U/ml, 0.5877 U/ml, and 0.7767 U/mL. The highest collagenase enzyme crude extract activity was produced at pH 8.5.

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Penggunaan tepung biji durian dan nangka pada berbagai level terhadap kualitas bakso daging itik petelur afkir

Jurnal Sains Peternakan ◽

10.21067/jsp.v7i1.3616 ◽

2019 ◽

Vol 7 (1) ◽

pp. 70-74

Author(s):

Fernia Rismawati ◽

Permata Ika Hidayati ◽

Dyah Lestari Yulianti

Keyword(s):

Field Experiment ◽

Low Level ◽

Organoleptic Test ◽

Seed Flour ◽

Randomized Design ◽

Completely Randomized Design ◽

Durian Seed

ABSTRAK Penelitian ini bertujuan untuk mengetahui penggunaan tepung biji durian dan nangka pada berbagi level terhadap kualitas bakso daging itik petelur afkir. Materi yang digunakan dalam penelitian ini daging itik petelur afkir sebagai bahan dasar pembuatan bakso, tepung biji buah durian, dan tepung biji buah nangka. Metode yang digunakan adalah percobaan lapangan dengan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 4 ulangan. Perlakuan yang digunakan adalah kontrol P0 (tanpa penambahan tepung biji durian atau tepung biji nangka), dan penambahan tepung P1 (5%), P2 (10%), P3 (15%), P4 (20%). Variabel yang diamati meliputi uji organoleptik dan daya susut masak. Hasil penelitian menunjukkan bahwa perlakuan penambahan kadar pati biji durian dan nangka pada tingkat signifikan 1% diperoleh hasil yang sangat signifikan (P<0,01) sehingga hipotesis diterima, yang berarti ada pengaruh penambahan tingkat pati. Penelitian ini menyimpulkan bahwa penggunaan tepung biji durian dan nangka 5% memiliki tingkat kesukaan tertinggi dan 20% memiliki tingkat kesukaan terendah. Abstract The research was aimed to test the use of durian and jackfruit seeds at various levels on the quality of duck laying duck meatballs.This research was conducted on 20 November 2016 until 20 December 2016. The method used was field experiment in a Completely Randomized Design (CRD) with 5 treatments and 4 replications. The treatment used is P0 Control (without the addition of durian seed flour or jackfruit seed flour), and addition of flour P1 (5%), P2 (10%), P3 (15%), and P4 (20%).The material used is durian seed flour, jackfruits seed flour, and laying duck laying. The observed variables include the organoleptic test and shrinking power.The results showed that treatment of the addition of seed starch level of durian and jackfruit at significant level of 1% obtained very significant results (P<0,01) so that the hypothesis is accepted, which means there is an influence on the addition of starch level. This research concluded that the use of durian and jackfruit flour 5% level has the highest level of favorite and the level of 20% has a low level of favorite.

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PENGARUH EKSTRA BUAH PALA MYRISTICA ARGENTHA TERHADAP PERTUMBUHAN DAN SINTASAN IKAN MAS KOI CYPRINUS CARPIO PADA DOSIS BERBEDA

Journal of Aquaculture and Environment ◽

10.35965/jae.v3i1.1064 ◽

2020 ◽

Vol 3 (1) ◽

pp. 19-22

Author(s):

Nur Yanti ◽

Sutia Budi ◽

Mardiana Mardiana

Keyword(s):

Growth Rate ◽

Cyprinus Carpio ◽

Crude Extract ◽

Fish Feed ◽

Fruit Extract ◽

Growth And Survival ◽

Randomized Design ◽

Test Parameters ◽

Completely Randomized Design ◽

Different Doses

Ikan koi di Indonesia merupakan ikan hias favorit dan banyak digemari masyarakat luas karena tubuhnya yang mempesona dan harganya relatif tidak terlalu mahal. Tujuan penelitian ini adalah untuk mengetahui pengaruh ekstrak kasar Buah Pala Myristica argentea terhadap pertumbuhan dan sintasan ikan Koi Cyprinus caprio. Hewan uji digunakan jenis ikan Mas Koi Cyprinus carpio berumur 2 bulan dengan ukuran 10 ± 3,6 cm. Kepadatan ikan Mas yang digunakan pada penelitian ini adalah 1 ekor./L. Wadah penelitian berupa toples transparan berukuran 5 L. Penelitian didesain dengan rancangan acak lengkap dengan 4 perlakuan dan masing – masing tiga ulangan. Perlakuan yang diujikan adalah perbedaan dosis ekstrak Pala dalam pakan yang diberikan, yaitu perlakuan A (0 %), perlakuan B (5 %), perlakuan C (10 %) dan perlakuan D (15 %). Parameter uji berupa laju pertumbuhan dan sintasan ikan Mas Koi. Hasil penelitian menunjukkan bahwa pemberian ekstrak buah Pala pada pakan ikan Mas Koi memberikan nilai yang baik dimana pada tingkat pertumbuhan dan sintasan menunjukkan nilai yang baik. Nilai ini menunjukkan respon ikan Mas Koi pada ekstrak kasar Pala dapat diterima. Koi fish in Indonesia is a favorite ornamental fish and much favored by the wider community because of its charming body and the price is relatively not too expensive. The purpose of this study was to determine the effect of the crude extract of Myristica argentea Nutmeg on the growth and survival of the Koi fish Cyprinus caprio. The test animals used the type of Mas Koi Cyprinus carpio aged 2 months with a size of 10 ± 3.6 cm. The density of goldfish used in this study was 1 fish./L. The research container was a transparent jar measuring 5 L. The study was designed in a completely randomized design with 4 treatments and three replications each. The treatments tested were the different doses of Nutmeg extract in the feed given, namely treatment A (0 %), treatment B (5 %), treatment C (10%) and treatment D (15 %). The test parameters were the growth rate and survival rate of Mas Koi fish. The results showed that the administration of Nutmeg fruit extract on Mas Koi fish feed gave a good value where the growth rate and survival showed good values. This value indicates that the response of the Koi carp to the crude extract of nutmeg is acceptable.

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The Quality and Antioxidant Content of Salted Eggs Made By Addition of Bay Leave Crude Extract on the Salting Media

Advances in Tropical Biodiversity and Environmental Sciences ◽

10.24843/atbes.2019.v03.i02.p05 ◽

2019 ◽

Vol 3 (2) ◽

pp. 37

Author(s):

Kadek Karang Agustina ◽

Ida Ayu Putu Aselya Mardyawati ◽

I Ketut Suada

Keyword(s):

Antioxidant Capacity ◽

Crude Extract ◽

Leaf Extract ◽

Antioxidant Content ◽

Randomized Design ◽

Completely Randomized Design ◽

Haugh Unit

This research aims to produce salted eggs with antioxidant content from bay leave extract. The study used a completely randomized design (CRD) in which there were 3 treatments, namely, control with 0% bay leaf extract, treatment 1 with adding 25% crude extract of bay leaves, and treatment 2 with a 50% addition of crude bay leaf extract. All treatments were pressed for 14 days before testing. The parameters observed in the study were the antioxidant capacity, albumin index, yolk index, and Haugh unit. The results show that the sated eggs with the different concentration of bay leave extract contain antioxidant 4.445%, 30.853%, and 44.32% respectively, albumin index of treated eggs was lower than control, while Haugh unit of treated eggs was higher than control, and no differentiation of yolk index between the treatments. To conclude, the addition of bay leave crude extract can increase the value of salted eggs without disturbing the quality.

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Ekstraksi Flavour dari Tepung Ikan Layang (Decapterus sp.) Menggunakan Enzim Protease Biduri (Calotropis gigantea)

Jurnal FishtecH ◽

10.36706/fishtech.v9i1.11481 ◽

2020 ◽

Vol 9 (1) ◽

pp. 6-12

Author(s):

Jumardi Tondais ◽

David Engelbert Sombo ◽

Bella Anjelika Lalenoh ◽

Mappiratu Mappiratu ◽

Adrian Adrian ◽

...

Keyword(s):

Amino Acids ◽

Side Effects ◽

Human Health ◽

Hydrolysis Time ◽

Randomized Design ◽

Flying Fish ◽

Completely Randomized Design ◽

Natural Flavors ◽

Plant Sap ◽

Total Amino Acids

The development of science and technology has resulted in many mononatrium glutamate (MSG) flavorings which can have side effects for human health. The development of natural flavors needs to be done to reduce side effects for human health. Betell plant sap can be used as a source of protease enzymes that have the potential as active agents in flavor production. Flavors can be obtained from animal protein such as flying fish. The purpose of this study was to determine the best treatment of the enzyme biduri in producing flavored flying fish. This study uses a factorial completely randomized design (RALF) method with concentrations of 1.5%, 2.0%, and 2.5% slurry proteases with a hydrolysis duration of 30, 60, and 90 minutes. The results obtained indicate that the best water content at a concentration of 1.5% hydrolysis time is 90 minutes (47,84%), dissolved protein in concentration 2.5% hydrolysis time 60 minutes (86,83%), total amino acids in concentration 1.5% hydrolysis time 90 minutes (3,11%), and organoleptic value in concentration 1.5%.

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