scholarly journals Ekstraksi Flavour dari Tepung Ikan Layang (Decapterus sp.) Menggunakan Enzim Protease Biduri (Calotropis gigantea)

2020 ◽  
Vol 9 (1) ◽  
pp. 6-12
Author(s):  
Jumardi Tondais ◽  
David Engelbert Sombo ◽  
Bella Anjelika Lalenoh ◽  
Mappiratu Mappiratu ◽  
Adrian Adrian ◽  
...  
Keyword(s):  
Amino Acids ◽  
Side Effects ◽  
Human Health ◽  
Hydrolysis Time ◽  
Randomized Design ◽  
Flying Fish ◽  
Completely Randomized Design ◽  
Natural Flavors ◽  
Plant Sap ◽  
Total Amino Acids

The development of science and technology has resulted in many mononatrium glutamate (MSG) flavorings which can have side effects for human health. The development of natural flavors needs to be done to reduce side effects for human health. Betell plant sap can be used as a source of protease enzymes that have the potential as active agents in flavor production. Flavors can be obtained from animal protein such as flying fish. The purpose of this study was to determine the best treatment of the enzyme biduri in producing flavored flying fish. This study uses a factorial completely randomized design (RALF) method with concentrations of 1.5%, 2.0%, and 2.5% slurry proteases with a hydrolysis duration of 30, 60, and 90 minutes. The results obtained indicate that the best water content at a concentration of 1.5% hydrolysis time is 90 minutes (47,84%), dissolved protein in concentration 2.5% hydrolysis time 60 minutes (86,83%), total amino acids in concentration 1.5% hydrolysis time 90 minutes (3,11%), and organoleptic value in concentration 1.5%.

scholarly journals Performance, gut morphometry and enzymatic activity of broilers fed neonatal supplementation in housing

2018 ◽  
Vol 40 (1) ◽  
pp. 38423
Author(s):  
Tatiane Souza dos Santos ◽  
Adriano Barbieri ◽  
Robert Guaracy Aparecido Cardoso Araujo ◽  
Gustavo Do Valle Polycarpo ◽  
Daniela Felipe Pinheiro ◽  
...  
Keyword(s):  
Amino Acids ◽  
Polynomial Regression ◽  
Pancreatic Enzyme ◽  
Broiler Chicks ◽  
Quadratic Effect ◽  
Linear Effect ◽  
Intestinal Length ◽  
Randomized Design ◽  
Relative Organ Weight ◽  
Completely Randomized Design

The objective was to evaluate the performance, relative organ weight, morphometry, intestinal length and pancreatic enzyme activity of broilers receiving neonatal supplementation. A total of 900 Cobb 500 broiler chicks was housed in 30 boxes of 2.5 m². The treatments were divided in a completely randomized design with six replications, and consisted of five inclusion levels (0.0, 2.5, 5.0, 7.5 and 10 grams per bird). The supplement used was based of amino acids, vitamins and minerals, which was provided on the ration on the first day in the housing. Statistical analysis was performed using SAS (2008) and when significant broken down using a polynomial regression. Due to the inclusion of the neonatal supplement in the period from one to seven and one to 21 days of age, the feed intake presented a linear effect and the weight gain, a quadratic effect. The relative weights of the gizzard and liver showed a quadratic effect, and the inclusions of 4.18 and 3.41 grams/bird provided the lowest weights, respectively. The jejunum villi height decreased with supplementation as well as the activity of pancreatic lipase. It can be concluded that neonatal supplementation benefited the performance of birds up to 21 days of age.

scholarly journals Characteristic of Protein Hydrolyzate of Snakehead Fish (Channa striata) Head by Using the Treatment of Different Bromelin Enzyme Concentration and Hydrolysis Time

2019 ◽  
pp. 188-196
Author(s):  
Sri Udayana Tartar ◽  
M Mahendradatta ◽  
Prof. Mursalim ◽  
Prof. Adiansyah
Keyword(s):  
Enzyme Concentration ◽  
Fish Protein ◽  
Food Sector ◽  
Hydrolysis Time ◽  
Protein Hydrolyzate ◽  
Randomized Design ◽  
Channa Striata ◽  
Completely Randomized Design ◽  
The Difference ◽  
Hydrolysis Of

Snakehead fish (Channa striata) has fairly high protein content; hence it has the potential to be utilized as a fish protein hydrolyzate. Fish protein hydrolyzate has great benefits in a food sector; therefore in order to obtain functional food products from fish that meet the standards and to utilize snakehead fish head, then enzymatic hydrolyzate products are made using bromelin enzymes. The research was aimed to determine the physicochemical properties of protein hydrolysates of snakehead fish head. It uses a laboratory experimental using a Completely Randomized Design (CRD). The data obtained were analyzed using ANOVA with further testing to determine whether or not the value was influential with Tukey test. The treatment applied was the difference in protein hydrolyzate of snakehead fish head, i.e the weight of head meat of snakehead fish given the treatment of bromelin enzymes with different percentages (6, 8 and 10%) and with different hydrolysis times (8, 10 and 12 hours). The results of research showed that the best result of treatment were obtained on 10% bromelin enzyme by temperature hydrolysis of 600C for 8 hours with the criteria of a brownish-yellow protein hydrolyzate, and having a water content of 80.40 - 77.79 %, ash 0.65 - 0.12%, protein 21.11 - 18.49%, fat 0.84 - 0.65%, yield 52.22 - 43.69%, pH 7.0 - 6.7, brightness 29.93 -23.13.

scholarly journals The Effect of Amino Acids Lysine and Methionine on the Carcass of Native Chickens

2020 ◽  
Vol 4 (2) ◽  
pp. 157-161
Author(s):  
I Nengah Andri Bayu Dita Dita ◽  
Ni Ketut Sri Rukmini ◽  
Ni Made Yudiastari
Keyword(s):  
Amino Acids ◽  
Weight Gain ◽  
Analysis Of Variance ◽  
Carcass Weight ◽  
Not Given ◽  
Free Range ◽  
Randomized Design ◽  
Native Chicken ◽  
Completely Randomized Design ◽  
Native Chickens

This study aims to determine the effect of 1% lysine, 0.5% methionine and the combination of lysine and methionine (1% + 0.5%) in the ration on the weight gain of carcass parts of native chickens. The design used in this study was a completely randomized design (CRD) consisting of 4 treatments and 3 replications, while the treatments were R0 (control) native chickens that were not given additional lysine and methionine, R1 native chickens were given feed containing lysine 1 %, R2 of free-range chickens given feed containing 0.5% methionine, R3 of native chickens given feed containing lysine and methionine (1% + 0.5%). The parameters observed were the weight gain in the chest, thighs, wings, and back. The data obtained were analyzed by analysis of variance. The results showed that the provision of rations which were added with the combination of amino acids lysine and methionine had a very significant effect (P> 0.01) on the weight gain of the carcass, thighs and backs of native chickens aged 10 weeks. Based on the results of this study it can be concluded that the combination of the amino acids lysine and methionine in the ration gave the best effect on the weight gain of carcass parts of native chicken aged 10 weeks. Based on the results of the study, it is suggested to add the combination of lysine (1%) and methionine (0.5%) in the native chicken ration.   Keywords: native chickens, carcass weight, lysine, methionine

scholarly journals Profiling of Collagens from Swim Bladder of Catfish (Pangasius sp.) by Acid Extraction

2019 ◽  
Vol 22 (2) ◽  
pp. 327-339
Author(s):  
Gressty Sari Br Sitepu ◽  
Joko Santoso ◽  
Wini Trilaksani
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Triple Helix ◽  
Extraction Process ◽  
Raw Material ◽  
Acid Extraction ◽  
Swim Bladder ◽  
Acid Pretreatment ◽  
Randomized Design ◽  
Completely Randomized Design

Swim bladders of catfish contain high protein, therefore it is can be used as a raw material for collagen. The study aims to determine the chemical characteristics of swim bladders, the pretreatment of non-collagen, extraction of collagen dissolves acid and to evaluate the characteristics of collagen. The method of this study is KOH pretreatment with a concentration of 0,05; 0,1; and 0,15 for 12 hours. The extraction process is done by soaking the sample in a solution of acetic acid with a concentration of 0,25; 0,5 and 0,75 M (ratio 1:10; b/v) and extraction time for 24; 48; and 72 hours at 4oC. The experimental design used for alkaline and acetic acid pretreatment were factorial completely randomized design. The result showed that the protein content of swim bladder was 85,26% (db), the profile of amino acids were dominated by three amino acids namely glycine (56,85 mg/g), prolyne (31,03 mg/g), and alanyne (23,85 mg/g). Using 0,05 M KOH for 8 h was selected as the best pretreatment method for collagen extraction. Extraction method using 0,50 M acetic for 48 h resulted he best collagen which revealed the existence of a triple helix structure and had Tg 84oC.

scholarly journals Amino acids digestibility of pelleted microparticle protein of fish meal and soybean meal in broiler chickens

2018 ◽  
Vol 43 (2) ◽  
pp. 169 ◽  
Author(s):  
N. Suthama ◽  
P. J. Wibawa
Keyword(s):  
Amino Acids ◽  
Particle Size ◽  
Soybean Meal ◽  
Broiler Chickens ◽  
Fish Meal ◽  
Coconut Oil ◽  
Intact Protein ◽  
Calcium Retention ◽  
Randomized Design ◽  
Completely Randomized Design

Commom protein sources for poultry, fish meal and soybean meal, were ground to obtain reduced particle size. The particle was then dissolved in distilled water (1 : 4 w/v), and added with 2 mL virgin coconut oil for every 500 mL solution prior to ultrasound transducer (ultrasonic bath) treatment to obtain protein microparticle. Reducing particle size is one possible way to increase protein utilization.180 birds were used for forced feeding and 10 other birds were plotted for endogenous correction, when they were one month and a half old. Microparticle protein of both ingredients were tested separately in either mash or pelleted forms and compared to intact protein. Completely randomized design with 3 treatments (intact, mash, and pellet) and 6 replications (10 bidrs each) was arranged for the respective ingredient. Protein and essential amino acid digestibilities, and calcium retention were the parameters measured. Analysis of variance continued to Duncan test were applied to statistically evaluate the data. Pelleted microparticle protein of fish meal and soybean meal, respectively, resulted in significantly (P<0.05) highest protein and amino acids digestibilities, and Ca retention although lower disgestibility of fewer amino acids was found in mash form. In conclusion, pelleted form of microparticle protein of either fish meal or soybean meal improve protein and mostly amino acids digestibilities, and calcium retention in broiler. 

scholarly journals PENGGUNAAN BERBAGAI TEKANAN DAN WAKTU HIDROLISIS PADA PRODUKSI GLUKOSAMIN HIDROKLORIDA DARI KITOSAN CANGKANG BEKICOT (Achatina fulica)

KOVALEN ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Noviana Linawati Dewi ◽  
Syaiful Bahri ◽  
Jaya Hardi
Keyword(s):  
Factorial Design ◽  
Time Pressure ◽  
Distilled Water ◽  
Achatina Fulica ◽  
Hydrolysis Time ◽  
Glucosamine Hydrochloride ◽  
Snail Shell ◽  
Randomized Design ◽  
Completely Randomized Design

ABSTRACT This study aims to determine the effect of pressure and time hydrolysis to produce glucosamine hydrochloride with the highest yield and the best quality of the chitosan of a snail shell. The study used a completely randomized design (CRD) factorial design consisting of 2 factors with 5 variations of hydrolysis time (40; 50; 60; 70 and 80) minutes and 2 variations of pressure (1 atm and 2 atm). Each treatment is done twice so obtained 20 experimental units. The highest glucosamine hydrochloride was obtained in hydrolysis time of 80 minutes and a pressure of 1 atm. Yield of 7,54%. Test of solubility in distilled water at 20°C was obtained of 3,79. Mol/L, while the maximum absorption of the UV-Vis with the addition of reagent Schales is at a wavelength of 420 nm. Keywords: Snails, Chitosan, Glucosamine, Hydrolysis Time, Pressure

scholarly journals The Extract Welch Starfruit Leaves Of Water Levels, Proteins And Fats Skipjack (Katsuwonus Pelamis ) Smoke

2019 ◽  
Vol 2 (1) ◽  
pp. 1-7
Author(s):  
Ida Astuti ◽  
Asniati Ningsih
Keyword(s):  
Energy Source ◽  
Amino Acids ◽  
Water Content ◽  
Nutrient Content ◽  
Water Levels ◽  
Katsuwonus Pelamis ◽  
Food Ingredients ◽  
Living Things ◽  
Randomized Design ◽  
Completely Randomized Design

Some food ingredients are needed by living things because they contain food substances needed in growth. This substance is commonly called the nutrient content of the food. This substance is very important, especially in metabolic processes such as breaking protein into amino acids needed for the growth of babies, children, and also helps maintain the function of carbohydrates as an energy source. The purpose of this study was to determine the effect of extracts on nutrient levels of skipjack (Katsu wonu spelamis) fufu (smoke). This study used a completely randomized design, with two treatments (the use of preservatives and without preservatives) with 3 replications. Based on the results of the study it was found that the extract of the welch starfruit leaves did not significantly affect the protein and fat content but significantly affected the water content.

scholarly journals Biostimulants Based on Humic Acids, Amino Acids and Vitamins Increase Growth and Quality of Lettuce Seedlings

2019 ◽  
Vol 11 (6) ◽  
pp. 235
Author(s):  
Rubens Ribeiro da Silva ◽  
Antonio Carlos Martins dos Santos ◽  
Jefferson Santana da Silva Carneiro ◽  
Lara Couto Marques ◽  
Larissa Urzêdo Rodrigues ◽  
...  
Keyword(s):  
Amino Acids ◽  
Plant Growth ◽  
Factorial Design ◽  
Humic Acids ◽  
Lactuca Sativa L ◽  
Randomized Design ◽  
Production Research ◽  
Completely Randomized Design ◽  
The Ideal

Humic acids (HA), amino acids (AA) and vitamins can increase plant growth; however, there are still few studies that determine the ideal concentration for use in lettuce (Lactuca sativa L.) production. Research was conducted to evaluate the effects of HA enriched with sources and levels of AA and vitamins on lettuce seedlings morphology and quality. Two experiments were conducted in a completely randomized design with four replicates. The first experiment had 16 treatments, 3 &times; 5 + 1 factorial design, the first factor was betaine, glycine and proline used in the HA enrichment, and second factor five concentrations of AA (0, 0.5, 1.0, 1.5, and 2.0 g L-1), plus the control, without HA and AA. The second experiment had 21 treatments, 4 &times; 5 + 1 factorial design, the first factor was the use of four vitamins (A, E, B1 and B2) in the enrichment of HA, while the second factor was composed of five concentrations of each vitamin (0, 0.5; 1.0, 1.5, and 2.0 g L-1) plus the control, without HA and vitamins. The application of HA and HA+AA promote the improvement in the seedling&rsquo;s morphology and quality, while the application of the HA+vitamins negatively affected the morphology, reducing seedling quality.

Apparent and standardised digestibility in broilers fed flaxseed meal with multicarbohydrase and phytase at 35 days of age

2021 ◽  
Author(s):  
Robert Guaracy Aparecido Cardoso Araujo ◽  
Connie Gallardo Vela ◽  
José Roberto Sartori ◽  
Messias Alves Trindade Neto
Keyword(s):  
Amino Acids ◽  
Factorial Design ◽  
Dry Matter ◽  
Energy Use ◽  
Ileal Digestibility ◽  
Flaxseed Meal ◽  
Randomized Design ◽  
Calcium Phosphorus ◽  
Completely Randomized Design ◽  
To Receive

The apparent total tract digestibility (ATTD; trial 1) and the apparent (AID) and standardized (SID) ileal digestibility of the amino acids (AA) (trial 2) in flaxseed meal (FM) were evaluated with the addition of exogenous multi-carbohydrase (MC) and phytase (Phy). A total of 80 28-day-old broilers were allotted in a completely randomized design to receive treatments up to 35 days of age. A 2 × 2 factorial design as used to determine the enzyme effects, on the ATTD of dry matter, nitrogen, calcium, phosphorus and fibre; energy use and the AID and SID of AA, in five replicate cages. There was synergism between MC and Phy on the ATTD of nitrogen and energy. The isolated inclusion of MC and Phy on the ATTD of dry matter, calcium, phosphorus and fibre of FM. The enzyme inclusion on the AID and SID of AA in FM, established by comparing the means, suggested a better response to the addition of MC + Phy. Supplementation with Phy or combination of MC and Phy was a viable alternative to increase the ATTD of nutrients and energy. The Combination of MC + Phy resulted in higher apparent and standardised digestibility of amino acidsAA from FM.

scholarly journals Efficiency of inert mineral dusts in the control of corn weevil

2016 ◽  
Vol 20 (2) ◽  
pp. 158-162
Author(s):  
Carlos F. Jairoce ◽  
Cristiano M. Teixeira ◽  
Adrise M. Nunes ◽  
Daniela R. Holdefer ◽  
Alexandra P. Krüger ◽  
...  
Keyword(s):  
Mortality Rate ◽  
Human Health ◽  
Diatomaceous Earth ◽  
Sitophilus Zeamais ◽  
Randomized Design ◽  
Inert Dusts ◽  
Mineral Dusts ◽  
Temperature Controlled ◽  
Completely Randomized Design ◽  
Corn Grains

ABSTRACT Corn weevil (Sitophilus zeamais) may cause great losses in the crop and in stored corn grains. This insect is controlled with the use of chemical insecticides, which may cause serious damage to human health. One alternative of control is the use of inert dusts. The objective of this study was to evaluate the efficiency of inert dusts in the control of S. zeamais under laboratory conditions. The experiment was conducted in 2014, in a completely randomized design, and the treatments consisted of basalt dust with three different granulometries (A, B and C) and diatomaceous earth, each of which at the doses of 2 and 4 kg t-1 and a control (no application). Each treatment had four replicates, and the sample unit consisted of 20 g of corn grains infected with 10 adults of S. zeamais kept in temperature-controlled chamber at 25 °C, 70% RH and photophase of 12 h. The dust efficiency was calculated using the equation of Abbott. The mortality rate was higher with the use of diatomaceous earth, reaching 100% after 5 days of exposure and the percentage of control for basalt dusts, 29 days after treatment, was above 80%.

Sign in / Sign up

Export Citation Format

Share Document