scholarly journals Cold stress in soybean (Glycine max L.) roots: Exogenous gallic acid promotes water status and increases antioxidant activities

2019 ◽  
Vol 43 (1) ◽  
pp. 59-71 ◽  
Author(s):  
Ceyda Ozfidan-Konakci ◽  
Evren Yildiztugay ◽  
Aysegul Yildiztugay ◽  
Mustafa Kucukoduk
Keyword(s):  
Lipid Peroxidation ◽  
Glycine Max ◽  
Cold Stress ◽  
Gallic Acid ◽  
Antioxidant Activities ◽  
Water Status ◽  
Monodehydroascorbate Reductase ◽  
Antioxidant Systems ◽  
Sod Activity ◽  
Soybean Roots

Gallic acid (GLA; 3,4,5- trihydroxybenzoic acid) is a strong antioxidant in plants. In order to clarify the effects of GLA as a pro-oxidant or an antioxidant on cells under stress conditions, soybean (Glycine max) was grown under normal conditions or in the presence of cold stress (5 and 10?C) in the absence or presence of gallic acid (GLA; 1 and 2 mM) for 72 h. The soybean roots exposed to stress exhibited a significant decline in growth (RGR), water content (RWC), osmotic potential (??) and proline content (Pro). However, GLA treatment under stress significantly improved these parameters and alleviated the stress-generated damage. Stress decreased superoxide dismutase (SOD) activity, but GLA effectively mitigated the adverse effects on enzyme activity. After stress treatment, only catalase (CAT) was induced in soybean roots, although it was not sufficient to prevent toxic hydrogen peroxide (H2O2) accumulation. Thus, the levels of lipid peroxidation (TBARS content) markedly increased. However, GLA contributed to detoxification of H2O2 and lipid peroxidation by enhancing activities of CAT and peroxidase (POX). In addition to these enzymes, SOD activity was able to scavenge superoxide anion radicals, as evidenced by decline in TBARS content. However, monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), total ascorbate (tAsA) and glutathione (GSH) showed a decline of content in roots treated with GLA (both concentrations) plus stress. Our results suggest a protective role of GLA, which may strengthen plant tolerance by ensuring efficient water use and enhancing antioxidant systems. In soybean roots, GLA successfully alleviated the toxicity of cold stress by modulating the activities of SOD, CAT and POX rather than enzymes of the ascorbate-glutathione cycle.

Antiulcer and Antioxidant Activity of a Lectin from Mucuna pruriens Seeds on Ethanol- induced Gastropathy: Involvement of Alpha-2 Adrenoceptors and Prostaglandins

2019 ◽  
Vol 25 (12) ◽  
pp. 1430-1439 ◽  
Author(s):  
Isabela Ribeiro Pinto ◽  
Hellíada V. Chaves ◽  
Auriana S. Vasconcelos ◽  
Francisca Clea F de Sousa ◽  
Tatiane Santi-Gadelha ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Acute Toxicity ◽  
Antioxidant Activities ◽  
Mucuna Pruriens ◽  
Toxicity Tests ◽  
Sod Activity ◽  
Tropical Regions ◽  
Mediated Effects ◽  
Menstruation Disorders ◽  
Therapeutic Tools

Ethnopharmacological Relevance:Mucuna pruriens (Mp) belongs to Leguminosae family, it is native of tropical regions and used to treat several maladies such as urinary, neurological, and menstruation disorders, constipation, edema, fever, tuberculosis, ulcers, diabetes, arthritis, dysentery, and cardiovascular diseases. Mp seeds are rich in bioactive compounds, for instance, lectins, a heterogeneous group of proteins and glycoproteins with a potential role as therapeutic tools for several conditions, including gastric disorders. This study investigated the acute toxicity, gastroprotective, and antioxidant activities of a lectin from Mucuna pruriens seeds (MpLec) on ethanol-induced gastropathy model in mice.Materials & Methods:Mice received MpLec (5 or 10 mg/kg; i.v.) and were observed for acute toxicity signs; in another experimental series, mice were pre-treated with MpLec (0.001; 0.01 or 0.1 mg/kg, i.v.), ranitidine (80 mg/kg, p.o.), or saline (0.3 mL/30g, i.v.) before ethanol 99.9% (0.2 mL/animal, p.o.), and euthanized 30 min after ethanol challenge. Macroscopic and microscopic gastric aspects, biochemical parameters (tissue hemoglobin levels, iron-induced lipid peroxidation, GSH content, SOD activity, and gastric mucosal PGE2) were measured. Additionally, pharmacological tools (yohimbine, indomethacin, naloxone, L-NAME) were opportunely used to clarify MpLec gastroprotective mechanisms of action.Results:No toxicity signs nor death were observed at acute toxicity tests. MpLec reduced ethanol-induced gastric damage, edema, and hemorrhagic patches formation, as well as decreased lipid peroxidation, SOD activity, and increased GSH content. Yohimbine and indomethacin prevented MpLec effects, suggesting the involvement of alpha-2 adrenoceptors and prostaglandins in the MpLec-mediated effects.Conclusion:MpLec does not present toxicity signs and shows gastroprotective and antioxidant activities via alpha-2 adrenoceptors and prostaglandins in the ethanol-induced gastropathy model.

Influence of commonly used clinical antidotes on antioxidant systems in human hepatocyte culture intoxicated with α-amanitin

2010 ◽  
Vol 30 (1) ◽  
pp. 38-43 ◽  
Author(s):  
Jan Magdalan ◽  
Aleksandra Piotrowska ◽  
Agnieszka Gomułkiewicz ◽  
Tomasz Sozański ◽  
Adam Szeląg ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Culture Medium ◽  
Antioxidant Properties ◽  
Human Hepatocyte ◽  
Hepatocyte Culture ◽  
Antioxidant Systems ◽  
Effective Protection ◽  
Sod Activity ◽  
Severe Hepatotoxicity ◽  
Primary Mechanism

α-Amanitin (α-AMA) is the main toxin of Amanita phalloides and its subspecies (A. virosa and A. verna). The primary mechanism of α-AMA toxicity is associated with protein synthesis blocking in hepatocytes. Additionally, α-AMA exhibits prooxidant properties that may contribute to its severe hepatotoxicity. The aim of the present study was to assess the effect of α-AMA on lipid peroxidation and the activities of superoxide dismutase (SOD) and catalase (CAT) in human hepatocyte culture. The effects of benzylpenicillin (BPCN), N-acetyl-L-cysteine (ACC), and silibinin (SIL) on SOD and CAT activities and on lipid peroxidation in human hepatocyte culture intoxicated with α-AMA were also examined. In human hepatocyte culture, 48-hour exposure to α-AMA at a 2-μM concentration caused an increase in SOD activity, a reduction of CAT activity, and a significant increase in lipid peroxidation. Changes in SOD and CAT activity caused by α-AMA could probably enhance lipid peroxidation by increased generation of hydrogen peroxide combined with reduced detoxification of that oxygen radical. The addition of antidotes (ACC or SIL) to the culture medium provided more effective protection against lipid peroxidation in human hepatocytes intoxicated with α-AMA than the addition of BPCN, possessing no antioxidant properties.

A Study on the Antioxidant Activity and Tissues Selective Inhibition of Lipid Peroxidation by Saponins from the Roots of Platycodon grandiflorum

2009 ◽  
Vol 37 (05) ◽  
pp. 967-975 ◽  
Author(s):  
Xian-Jun Fu ◽  
Hong-Bing Liu ◽  
Peng Wang ◽  
Hua-Shi Guan
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Selective Inhibition ◽  
Antioxidant Systems ◽  
Platycodon Grandiflorum ◽  
Platycodi Radix ◽  
Antioxidant Effects ◽  
Different Tissues ◽  
Selective Effects

Platycodi Radix is the root of Platycodon grandiflorum (Jacq.) A. DC and has been used as a traditional medicine in China. According to the theory of traditional Chinese medicine, Platycodi Radix (PR) possesses the character of lung meridian tropism (Guijing) and has selective effects on the lung and respiratory system. The aim of this study was to confirm the antioxidant effects of saponins from Platycodi Radix (PRS), with emphasis on its selective inhibition of lipid peroxidation in different tissues. The hydroxyl radical scavenging activity was determined by a H2O2/Fe2+ system; a modified thiobarbituric acid reactive species assay was used to measure the lipid peroxide in rats' tissues. These antioxidant activities were compared to ascorbic acid (Vc). The results showed that PRS had antioxidant activities in various antioxidant systems. And the inhibition capability of lipid peroxidation of PRS and Vc were excellent, but differed greatly in different tissues. These results suggested that PRS had antioxidant effects and selective inhibition of lipid peroxidation. It indicated that the mechanism of Platycodi Radix to treat some diseases might be related to its antioxidant activity, especially its tissue selective effects. However, further study is needed.

scholarly journals Antioxidant activity of ethanolic extract of three Selaginella species from Java Island, Indonesia

2019 ◽  
Vol 20 (12) ◽  
Author(s):  
M Miftahudin ◽  
Rini Hasibuan ◽  
Tatik Chikmawati
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Sod Activity ◽  
Different Doses

Abstract. Miftahudin, Hasibuan RS, Chikmawati T. 2019. Antioxidant activity of ethanolic extract of three Selaginella species from Java Island, Indonesia. Biodiversitas 20: 3715-3722. Three Selaginella species, S. ornata, S. plana, and S. willdenowii, from Java Island, Indonesia, have been known to have antioxidant properties; however, in vivo antioxidant activities of these species have not been reported. This research aimed to evaluate the in vivo antioxidant activity of ethanolic extract of three Selaginella species. The 70% ethanol extract of three Selaginella species at four different doses was administered to mice one day before being treated with oxidative stress. The liver tissue of mice treated with or without oxidative stress was analyzed their lipid peroxidation by measuring MDA concentration and Superoxide Dismutase (SOD) activities. The results showed that there were variations in antioxidant activity among the three Selaginella species. In general, the dose of 0.3 g extract kg-1 BW has been able to reduce lipid peroxidation and increase SOD activity. The administration of S. ornata extract to the mice at 1.2 g extract kg-1 BW reduced the MDA concentration to the lowest level, but the same dose of two other Selaginella extracts caused toxic effects in mice. The antioxidant activities of S. ornata and S. plana were better than that of S. willdenowii extract, and among those species, S. ornata has the best antioxidant activity.

Modulations by dietary restriction on antioxidant enzymes and lipid peroxidation in developing mice

2003 ◽  
Vol 94 (3) ◽  
pp. 947-952 ◽  
Author(s):  
Aiguo Wu ◽  
Xiufa Sun ◽  
Fada Wan ◽  
Yugu Liu
Keyword(s):  
Lipid Peroxidation ◽  
Food Intake ◽  
Dietary Restriction ◽  
Body Weight Gain ◽  
Antioxidant Systems ◽  
Sod Activity ◽  
Male And Female ◽  
Female Mice ◽  
Gpx Activity ◽  
Developing Mice

The effects of dietary restriction (DR) on the activities of liver superoxide dismutase (SOD), catalase (Cat), and glutathione peroxidase (GPX) and the level of lipid peroxidation (LP) in developing mice were investigated in this study. Male and female Kunmin mice were fed a standard rodent diet ad libitum (AL), 80% of AL food intake (20% DR), or 65% of AL food intake (35% DR) for 12 or 24 wk. Both 12 and 24 wk of DR resulted in retarded body weight gain in male and female mice. The activities of SOD, Cat, and GPX and the content of LP in DR male and female mice were not different ( P > 0.05) from those in controls after 12 wk of DR. However, the SOD activity was increased at 24 wk in 20% DR ( P < 0.05) and 35% DR ( P < 0.01) male, but not in DR female, mice. The Cat activity was elevated at 24 wk in both DR male ( P < 0.05 for 20% DR, P < 0.01 for 35% DR) and female ( P < 0.01) mice with a greater increase in DR female ( P < 0.05) than in DR male animals. GPX activity was also increased at 24 wk in DR male ( P < 0.01) and female ( P < 0.01) mice with a greater elevation in DR females ( P < 0.05) than in DR males. Furthermore, LP was decreased at 24 wk in both DR male ( P < 0.01) and female ( P < 0.01) animals with a greater reduction in DR females ( P< 0.01) compared with DR males. These findings indicated that 24 wk, but not 12 wk, of DR led to differential effects on liver SOD, Cat, and GPX activities and LP content in male and female mice during development, suggesting sex-associated modulations of DR on antioxidant systems in developing animals.

scholarly journals Peroxidase and lipid peroxidation of soybean roots in response to p-coumaric and p-hydroxybenzoic acids

2003 ◽  
Vol 46 (2) ◽  
pp. 193-198 ◽  
Author(s):  
Patrícia Minatovicz F. Doblinski ◽  
Maria de Lourdes L. Ferrarese ◽  
Domitila A. Huber ◽  
Carlos Alberto Scapim ◽  
Alessandro de Lucca e Braccini ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Cell Wall ◽  
Glycine Max ◽  
Root Growth ◽  
Root Length ◽  
Dry Weight ◽  
Fresh Weight ◽  
Glycine Max L ◽  
Hydroxybenzoic Acids ◽  
Soybean Roots

The scope of the present study was to investigate how the p-coumaric (p-CA) and p-hydroxybenzoic (p-HD) acids affect the peroxidase (POD, EC 1.11.1.7) activity, the lipid peroxidation (LP) and the root growth of soybean (Glycine max (L.) Merr.). Three-day-old seedlings were cultivated in nutrient solution containing p-CA or p-HD (0.1 to 1 mM) for 48 h. After uptake, both compounds (at 0.5 and 1 mM) decreased root length (RL), fresh weight (FW) and dry weight (DW) while increased soluble POD activity, cell wall (CW)-bound POD activity (with 1 mM p-CA and 0.5 mM p-HD) and LP.

scholarly journals Antioxidant Activities in Sweet Potatoes Leaves Steamed with Spices

2020 ◽  
Vol 9 (4) ◽  
pp. 41
Author(s):  
Edith A. Agbo ◽  
Konan Kouassi ◽  
Désirée A. Gouekou ◽  
Souleymane Méité ◽  
Albarin G. Gbogouri ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Sweet Potato ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Total Phenolic Compounds ◽  
Sweet Potato Leaves

Sweet potato leaves contain phenolic and flavonoids compounds which give them a potential antioxidant capacity. However, these antioxidant activities can be lost during cooking due to high temperature. In the aim to enhance their antioxidant activities, sweet potato leaves were steamed with antioxidant spices. The spices were used alone or mixed: nutmeg, Guinea pepper, cloves, nutmeg-Guinea pepper, nutmeg-cloves, Guinea pepper-cloves and nutmeg-Guinea pepper-cloves. The phytochemical analysis was carried-out on total phenolic compounds and flavonoids, while the antioxidant activities were determined via free radical-scavenging and inhibition power of lipid peroxidation. The results showed that cloves total phenolic content (513.33 mg Gallic Acid Equivalent (GAE)/g dry matter (DM)) increased to 1786.67 mg GAE/g DM when mixed with Guinea pepper. Flavonoids content was high in Guinea pepper-cloves (57.17 mg Quercetin Equivalent (QE)/g DM). In sweet potato steamed leaves, total phenolic compounds content is improved by incorporating cloves (625.83 mg GAE/g DM), while flavonoids content is slightly improved by Guinea pepper (125.00 mg QE/g DM). Among spices, cloves, Guinea pepper-cloves and nutmeg IC50 values (0.45, 0.83 and 1.50 &mu;g/ml respectively) did not differ significantly to that of vitamin C (1.67 &mu;g/ml), indicating that they had a good antiradical activity. The capacity of spices and steamed sweet potato leaves with and without spices to inhibit lipid peroxidation was higher than that of Gallic acid which is the standard reference. Spices, particularly cloves and Guinea pepper can improve antioxidant activities during sweet potato leaves steaming.

Antioxidant Activities of Three Dihydrochalcone Glucosides from Leaves of Lithocarpus pachyphyllus

2004 ◽  
Vol 59 (7-8) ◽  
pp. 481-484 ◽  
Author(s):  
Wei-Min Yang ◽  
Ji-Kai Liu ◽  
Xiang-Dong Qin ◽  
Wan-Lin Wu ◽  
Zhi-He Chen
Keyword(s):  
Lipid Peroxidation ◽  
Inhibitory Concentration ◽  
Antioxidant Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Liver Homogenate ◽  
Effective Concentration ◽  
Scavenging Activity ◽  
Sod Activity

AbstractIn vitro antioxidant activities of three sweet dihydrochalcone glucosides from the leaves of Lithocarpus pachyphyllus (Kurz) Rehd. (Fagaceae), trilobatin 2″-acetate (1), phloridzin (2) and trilobatin (3), were investigated. The IC50 (50% inhibitory concentration) values for compounds 1-3 of lipid peroxidation in rat liver homogenate were 261, 28, 88 μm, respectively. Compounds 1-3 increased superoxide dismutase (SOD) activity with EC50 (50% effective concentration) values of 575, 167, 128 μm, and glutathione peroxidase (GSH-Px) activity with EC50 values of 717, 347, 129 μm, respectively, and showed only weak DPPH (1,1- diphenyl-2-picrylhydrazyl) radical scavenging activity

scholarly journals Prophylactic Antioxidant Potential of Gallic Acid in Murine Model of Sepsis

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Harikesh Maurya ◽  
Vaishali Mangal ◽  
Sanjay Gandhi ◽  
Kathiresan Prabhu ◽  
Kathiresan Ponnudurai
Keyword(s):  
Lipid Peroxidation ◽  
Gallic Acid ◽  
Large Population ◽  
Cecal Ligation ◽  
Sepsis Group ◽  
Acid Pretreatment ◽  
Sod Activity ◽  
Group Iii ◽  
Beneficial Effects ◽  
Group I

Present study is to investigate the effect of Gallic acid pretreatment on survival of septic animals and oxidative stress in different organs like lungs, liver, kidney, spleen, and vascular dysfunction of mice. Sepsis was induced by cecal ligation and puncture (CLP) in healthy adult male albino mice (25–30 g) and was divided into 3 groups each consisting of 6 animals, that is, sham-operated (SO group (Group I), SO + sepsis (Group II), and Gallic acid + sepsis (Group III)). Group III animals were pretreated with Gallic acid at the dose rate of 20 mg/kg body weight for 2 days before induction of sepsis. Animals were sacrificed on 8th day and the tissue samples were obtained for further investigation on lipid peroxidation (LPO), malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GSH). Gallic acid pretreatment significant (P<0.05) reduces kidney, spleen, liver, and lungs’ malondialdehyde level in septic mice. However, it fails to improve reduced glutathione level in all given organs, while, Gallic acid pretreated mice showed significant improvement in SOD activity of kidney and spleen when compared to septic mice. Finally, the beneficial effects of Gallic acid pretreatment in sepsis are evident from the observations that Gallic acid partially restored SOD and catalase activity and completely reversed lipid peroxidation. Further studies are required to find out the possible mechanisms underlying the beneficial effects of Gallic acid on large population.

scholarly journals The Apoplastic and Symplastic Antioxidant System in Onion: Response to Long-Term Salt Stress

Antioxidants ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 67 ◽  
Author(s):  
Grisaly García ◽  
María José Clemente-Moreno ◽  
Pedro Díaz-Vivancos ◽  
Marina García ◽  
José Antonio Hernández
Keyword(s):  
Salt Stress ◽  
Redox State ◽  
Reduced Glutathione ◽  
Membrane Integrity ◽  
Monodehydroascorbate Reductase ◽  
Antioxidant Systems ◽  
Lower Sensitivity ◽  
Sod Activity ◽  
Leaf Tissues

The response of apoplastic antioxidant systems in root and leaf tissues from two onion genotypes (‘Texas 502’, salt-sensitive and ‘Granex 429’, salt-resistant) in response to salinity was studied. Electrolyte leakage data indicated the membrane integrity impairing by the effect of salts, especially in ‘Texas 502’. We detected superoxide dismutase (SOD) and peroxidase (POX) activity in the root and leaf apoplastic fractions from onion plants. Salinity increased SOD activity in the root symplast of ‘Texas 502’ and in ‘Granex 429’ leaves. In contrast, salinity reduced SOD activity in the leaf and root apoplastic fractions from ‘Texas 502’. In ‘Granex 429’, salt-stress increased leaf apoplastic POX activity and symplastic catalase (CAT) activity of both organs, but a decline in root apoplastic POX from ‘Texas 502’ took place. Salt-stress increased monodehydroascorbate reductase (MDHAR) in root and leaf symplast and in root glutathione reductase GR, mainly in ‘Granex 429’, but only in this genotype, leaf dehydroascorbate reductase (DHAR) activity increased. In contrast, a decline in leaf GR was produced only in ‘Texas 502’. Salinity increased leaf ASC levels, and no accumulation of dehydroascorbate (DHA) was observed in roots in both cases. These responses increased the redox state of ascorbate, especially in roots. In contrast, salinity declined reduced glutathione (GSH), but oxidised glutathione (GSSG) was accumulated in leaves, decreasing the redox state of glutathione. Salinity slightly increased root GSH concentration in the salt-tolerant genotype and was unchanged in the salt-sensitive genotype, but no accumulation of GSSG was produced, favoring the rise and/or maintenance of the redox state of the glutathione. These results suggest that the lower sensitivity to salt in ‘Granex 429’ could be related to a better performance of the antioxidant machinery under salinity conditions.

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